{"id":65841,"date":"2026-10-10T14:00:37","date_gmt":"2026-10-10T06:00:37","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?post_type=ftc_question&#038;p=65841"},"modified":"2026-10-10T14:00:37","modified_gmt":"2026-10-10T06:00:37","slug":"cross-cohort-platform-species-ferroptosis-comparison","status":"publish","type":"ftc_question","link":"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/cross-cohort-platform-species-ferroptosis-comparison\/","title":{"rendered":"\u4e0d\u540c\u961f\u5217\u3001\u5e73\u53f0\u6216\u7269\u79cd\u7684\u94c1\u6b7b\u4ea1\u7ed3\u679c\u600e\u6837\u6bd4\u8f83\uff1f"},"content":{"rendered":"<p class=\"ftc-answer-first\"><strong>\u5148\u770b\u7ed3\u8bba\uff1a<\/strong>\u8de8\u961f\u5217\u6bd4\u8f83\u5e94\u5148\u5bf9\u9f50\u7814\u7a76\u95ee\u9898\u3001\u65b9\u5411\u548c\u7279\u5f81\u5b9a\u4e49\uff0c\u518d\u6bd4\u8f83\u5404\u81ea\u5206\u6790\u7684\u6548\u5e94\u4e0e\u4e0d\u786e\u5b9a\u6027\u3002\u4e0d\u540c\u5e73\u53f0\u7684\u539f\u59cb\u8868\u8fbe\u548c\u7b7e\u540d\u5206\u6570\u901a\u5e38\u4e0d\u80fd\u76f4\u63a5\u62fc\u5728\u540c\u4e00\u5c3a\u5ea6\u4e0a\u3002\u8de8\u7269\u79cd\u8fd8\u9700\u663e\u5f0f\u5904\u7406\u540c\u6e90\u6620\u5c04\uff1b\u6279\u6b21\u4e0e\u7ec4\u522b\u5b8c\u5168\u91cd\u5408\u65f6\uff0c\u6821\u6b63\u8f6f\u4ef6\u65e0\u6cd5\u4ece\u6570\u636e\u4e2d\u8bc6\u522b\u4e24\u8005\u5404\u81ea\u7684\u8d21\u732e\u3002<\/p>\n<h2>\u9002\u7528\u60c5\u51b5<\/h2>\n<p>\u5e0c\u671b\u628a GEO\u3001TCGA\u3001\u4e0d\u540c\u5b9e\u9a8c\u5e73\u53f0\u6216\u4eba\u9f20\u6570\u636e\u8054\u5408\u8d77\u6765\uff0c\u9a8c\u8bc1\u4e00\u4e2a\u94c1\u6b7b\u4ea1\u76f8\u5173\u7ed3\u8bba\u3002<\/p>\n<h2>\u5efa\u8bae\u6309\u8fd9\u4e2a\u987a\u5e8f\u68c0\u67e5<\/h2>\n<ol>\n<li>\u9010\u961f\u5217\u786e\u8ba4\u7ec4\u7ec7\u3001\u91c7\u6837\u65f6\u70b9\u3001\u6cbb\u7597\u80cc\u666f\u3001\u5bf9\u7167\u5b9a\u4e49\u548c\u7ed3\u5c40\uff0c\u5224\u65ad\u662f\u4e0d\u662f\u5728\u56de\u7b54\u540c\u4e00\u95ee\u9898\u3002<\/li>\n<li>\u7edf\u4e00 ID \u4e0e\u5019\u9009\u5b9a\u4e49\uff0c\u5206\u522b\u5b8c\u6210\u9002\u5408\u5e73\u53f0\u7684\u9884\u5904\u7406\u548c\u8d28\u63a7\uff0c\u8bb0\u5f55\u5404\u961f\u5217\u7b7e\u540d\u547d\u4e2d\u7387\u3002<\/li>\n<li>\u5148\u5728\u5404\u961f\u5217\u5185\u90e8\u4f30\u8ba1\u540c\u65b9\u5411\u6bd4\u8f83\u7684\u6548\u5e94\u53ca\u4e0d\u786e\u5b9a\u6027\uff0c\u518d\u51b3\u5b9a\u662f\u5426\u5177\u5907\u6c47\u603b\u6761\u4ef6\u3002<\/li>\n<li>\u8de8\u7269\u79cd\u4f7f\u7528\u6ce8\u660e\u7248\u672c\u7684\u540c\u6e90\u6620\u5c04\uff0c\u5355\u5217\u4e00\u5bf9\u591a\u3001\u672a\u6620\u5c04\u548c\u7269\u79cd\u7279\u5f02\u57fa\u56e0\uff0c\u5e76\u505a\u6620\u5c04\u9009\u62e9\u7684\u654f\u611f\u6027\u5206\u6790\u3002<\/li>\n<li>\u5916\u90e8\u9884\u6d4b\u9a8c\u8bc1\u8981\u51bb\u7ed3\u8bad\u7ec3\u6d41\u7a0b\uff1b\u4e0d\u80fd\u5728\u770b\u5230\u9a8c\u8bc1\u6807\u7b7e\u540e\u91cd\u65b0\u7b5b\u57fa\u56e0\u6216\u6311\u9009\u6700\u6709\u5229\u9608\u503c\u3002<\/li>\n<\/ol>\n<h2>\u5148\u5224\u65ad\u80fd\u6bd4\u8f83\u5230\u54ea\u4e00\u5c42<\/h2>\n<div class=\"ftc-table-wrap\" role=\"region\" tabindex=\"0\" aria-label=\"\u5148\u5224\u65ad\u80fd\u6bd4\u8f83\u5230\u54ea\u4e00\u5c42\">\n<table>\n<thead>\n<tr>\n<th scope=\"col\">\u7ec4\u5408<\/th>\n<th scope=\"col\">\u63a8\u8350\u6bd4\u8f83\u5bf9\u8c61<\/th>\n<th scope=\"col\">\u4e3b\u8981\u98ce\u9669<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\u540c\u75be\u75c5\u7684\u4e24\u4e2a\u4eba\u7fa4\u961f\u5217<\/td>\n<td>\u5404\u81ea\u6548\u5e94\u65b9\u5411\u3001\u6548\u5e94\u91cf\u53ca\u533a\u95f4<\/td>\n<td>\u5165\u7ec4\u6807\u51c6\u3001\u6cbb\u7597\u3001\u7ec8\u70b9\u4e0d\u540c<\/td>\n<\/tr>\n<tr>\n<td>\u82af\u7247\u4e0e RNA-seq<\/td>\n<td>\u5171\u540c\u53ef\u6d4b\u7279\u5f81\u4e0a\u7684\u4e00\u81f4\u6027<\/td>\n<td>\u63a2\u9488\u6620\u5c04\u548c\u5c3a\u5ea6\u4e0d\u540c<\/td>\n<\/tr>\n<tr>\n<td>\u4eba\u7c7b\u7ec4\u7ec7\u4e0e\u5c0f\u9f20\u5b9e\u9a8c<\/td>\n<td>\u4fdd\u5b88\u7a0b\u5e8f\u53ca\u5177\u4f53\u673a\u5236\u7684\u652f\u6301<\/td>\n<td>\u540c\u6e90\u5173\u7cfb\u4e0d\u7b49\u4e8e\u529f\u80fd\u76f8\u540c<\/td>\n<\/tr>\n<tr>\n<td>\u4e0d\u540c\u5355\u7ec6\u80de\u961f\u5217<\/td>\n<td>\u5339\u914d\u7ec6\u80de\u7c7b\u578b\u4e2d\u7684\u4f9b\u4f53\u5c42\u7ed3\u679c<\/td>\n<td>\u7ec6\u80de\u7ec4\u6210\u3001\u6ce8\u91ca\u548c\u6df1\u5ea6\u5dee\u5f02<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p class=\"ftc-inline-sources\">\u4f9d\u636e\uff1a<a href=\"https:\/\/static.ensembl.org\/info\/genome\/compara\/homology_types.html\" target=\"_blank\" rel=\"noopener noreferrer\">Ensembl\uff1a\u540c\u6e90\u57fa\u56e0\u5173\u7cfb\u7c7b\u578b<\/a> \u00b7 <a href=\"https:\/\/bioconductor.org\/packages\/release\/bioc\/vignettes\/DESeq2\/inst\/doc\/DESeq2.html\" target=\"_blank\" rel=\"noopener noreferrer\">DESeq2 \u5b98\u65b9\u6559\u7a0b\uff1a\u8f93\u5165\u3001\u8bbe\u8ba1\u77e9\u9635\u4e0e\u6280\u672f\u91cd\u590d<\/a><\/p>\n<h2>\u4e0d\u80fd\u9760\u6279\u6b21\u6821\u6b63\u6551\u56de\u7684\u8bbe\u8ba1<\/h2>\n<p>\u793a\u610f\u60c5\u5883\uff1a\u961f\u5217 A \u5168\u662f\u75be\u75c5\u6837\u672c\uff0c\u961f\u5217 B \u5168\u662f\u6b63\u5e38\u6837\u672c\u3002\u6b64\u65f6\u6765\u6e90\u4e0e\u7ec4\u522b\u5b8c\u5168\u91cd\u5408\u3002\u5373\u4f7f\u6821\u6b63\u540e PCA \u770b\u8d77\u6765\u6df7\u5408\uff0c\u4ecd\u4e0d\u80fd\u8bc1\u660e\u4f30\u8ba1\u51fa\u6765\u7684\u662f\u75be\u75c5\u6548\u5e94\uff1b\u7b97\u6cd5\u65e0\u6cd5\u51ed\u7a7a\u751f\u6210\u7f3a\u5931\u7684\u7ec4\u5185\u5bf9\u7167\u4fe1\u606f\u3002<\/p>\n<p>\u66f4\u53ef\u89e3\u91ca\u7684\u529e\u6cd5\u662f\u5bfb\u627e\u6bcf\u4e2a\u6765\u6e90\u5185\u90e8\u90fd\u6709\u76f8\u5e94\u6bd4\u8f83\u7684\u6570\u636e\uff0c\u5206\u522b\u5206\u6790\u540e\u8bc4\u4f30\u4e00\u81f4\u6027\uff0c\u6216\u628a\u73b0\u6709\u6570\u636e\u7528\u4e8e\u63cf\u8ff0\u800c\u964d\u4f4e\u63a8\u65ad\u8303\u56f4\u3002\u4e0d\u8981\u6309\u201c\u6d88\u6389\u6279\u6b21\u540e\u56fe\u597d\u770b\u201d\u51b3\u5b9a\u6821\u6b63\u662f\u5426\u6210\u529f\u3002<\/p>\n<h2>\u8de8\u7269\u79cd\u6620\u5c04\u7684\u6700\u5c0f\u8bb0\u5f55<\/h2>\n<p>\u8868\u5934\u5206\u6210\u591a\u884c\u4ec5\u4e3a\u9605\u8bfb\u793a\u610f\uff0c\u5b9e\u9645\u6587\u4ef6\u5e94\u4fdd\u6301\u4e00\u884c\u5b8c\u6574\u5b57\u6bb5\u3002\u82e5\u53ea\u4fdd\u7559\u4e00\u5bf9\u4e00\u540c\u6e90\u57fa\u56e0\uff0c\u5e94\u62a5\u544a\u56e0\u6b64\u4e22\u5931\u591a\u5c11\u5019\u9009\uff1b\u82e5\u7eb3\u5165\u4e00\u5bf9\u591a\u5173\u7cfb\uff0c\u5e94\u8bf4\u660e\u805a\u5408\u89c4\u5219\uff0c\u907f\u514d\u91cd\u590d\u7279\u5f81\u88ab\u65e0\u610f\u52a0\u6743\u3002\u4e0d\u540c\u961f\u5217\u5747\u503c\u4e2d\u5fc3\u5316\u540e\u7684\u5206\u6570\u4e5f\u53ea\u5177\u6709\u5bf9\u5e94\u8f6c\u6362\u540e\u7684\u542b\u4e49\u3002<\/p>\n<pre class=\"ftc-code\"><code>source_species\tsource_id\ttarget_species\ttarget_id\northology_type\tensembl_release\tkeep_rule\n\u7269\u79cdA\tDEMO_A\t\u7269\u79cdB\tDEMO_B\t\u5f85\u6838\u5bf9\t\u5f85\u586b\u5199\t\u9884\u5148\u6307\u5b9a<\/code><\/pre>\n<p class=\"ftc-inline-sources\">\u4f9d\u636e\uff1a<a href=\"https:\/\/static.ensembl.org\/info\/genome\/compara\/homology_types.html\" target=\"_blank\" rel=\"noopener noreferrer\">Ensembl\uff1a\u540c\u6e90\u57fa\u56e0\u5173\u7cfb\u7c7b\u578b<\/a><\/p>\n<h2>\u4e0d\u80fd\u4ece\u5f53\u524d\u7ed3\u679c\u76f4\u63a5\u5f97\u51fa\u4ec0\u4e48<\/h2>\n<p>\u6807\u51c6\u5316\u6216\u6279\u6b21\u6821\u6b63\u4e0d\u80fd\u4fee\u590d\u4e0d\u53ef\u8bc6\u522b\u7684\u7814\u7a76\u8bbe\u8ba1\u3002\u540c\u4e00\u7b7e\u540d\u8de8\u5e73\u53f0\u7edd\u5bf9\u5206\u6570\u76f8\u540c\u4e0d\u4ee3\u8868\u72b6\u6001\u76f8\u540c\uff1b\u89c2\u5bdf\u65b9\u5411\u4e00\u81f4\u4e5f\u4ecd\u4e0d\u662f\u673a\u5236\u56e0\u679c\u8bc1\u660e\u3002<\/p>\n<h2>\u4f9d\u636e\u4e0e\u7248\u672c<\/h2>\n<ul class=\"ftc-source-list\">\n<li><a href=\"https:\/\/static.ensembl.org\/info\/genome\/compara\/homology_types.html\" target=\"_blank\" rel=\"noopener noreferrer\">Ensembl\uff1a\u540c\u6e90\u57fa\u56e0\u5173\u7cfb\u7c7b\u578b<\/a><span>Compara \u5728\u7ebf\u6587\u6863\uff1b\u6620\u5c04\u9700\u53e6\u8bb0 release<\/span><\/li>\n<li><a href=\"https:\/\/bioconductor.org\/packages\/release\/bioc\/vignettes\/DESeq2\/inst\/doc\/DESeq2.html\" target=\"_blank\" rel=\"noopener noreferrer\">DESeq2 \u5b98\u65b9\u6559\u7a0b\uff1a\u8f93\u5165\u3001\u8bbe\u8ba1\u77e9\u9635\u4e0e\u6280\u672f\u91cd\u590d<\/a><span>release \u5728\u7ebf\u6559\u7a0b\uff1b\u793a\u4f8b\u5e94\u5339\u914d\u672c\u5730\u7248\u672c<\/span><\/li>\n<li><a href=\"https:\/\/www.tripod-statement.org\/resources\/\" target=\"_blank\" rel=\"noopener noreferrer\">TRIPOD \u5b98\u65b9\u8d44\u6e90\uff1a\u9884\u6d4b\u6a21\u578b\u5f00\u53d1\u4e0e\u9a8c\u8bc1\u6e05\u5355<\/a><span>2015 \u57fa\u7840\u8d44\u6599\u4e0e\u52d8\u8bef\uff1b\u73b0\u884c\u66f4\u65b0\u53e6\u89c1 TRIPOD+AI<\/span><\/li>\n<\/ul>\n<p class=\"ftc-verification\">\u6700\u540e\u6838\u9a8c\uff1a2026-10-10\uff5c\u8d44\u6599\u6838\u9a8c\uff1aAI \u7f16\u5199\u4e0e\u516c\u5f00\u6765\u6e90\u6838\u5bf9\uff1b\u672a\u7ecf\u4eba\u5de5\u4e13\u5bb6\u5ba1\u9605<\/p>\n<p class=\"ftc-verification\">\u4f9d\u636e\u516c\u5f00\u6765\u6e90\u6838\u5bf9\u6982\u5ff5\u3001\u8f93\u5165\u3001\u8bc1\u636e\u8fb9\u754c\u4e0e\u7248\u672c\u53e3\u5f84\uff1b\u793a\u610f\u6570\u636e\u4e0d\u4ee3\u8868\u771f\u5b9e\u7814\u7a76\u7ed3\u679c\u3002\u4ec5\u6807\u660e\u7684\u5c0f\u578b\u4ee3\u7801\u793a\u4f8b\u7ecf\u8fc7\u8fd0\u884c\u68c0\u67e5\uff0c\u4e0d\u4ee3\u8868\u5b8c\u6574\u751f\u7269\u4fe1\u606f\u6d41\u7a0b\u6216\u539f\u8bba\u6587\u5df2\u590d\u73b0\u3002<\/p>\n<h2>\u7ee7\u7eed\u89e3\u51b3\u76f8\u5173\u95ee\u9898<\/h2>\n<ul class=\"ftc-related-reading\">\n<li><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/counts-tpm-normalized-matrix-input\/\">count\u3001TPM\u3001\u6807\u51c6\u5316\u77e9\u9635\u5206\u522b\u80fd\u7528\u4e8e\u54ea\u4e9b\u94c1\u6b7b\u4ea1\u5206\u6790\uff1f<\/a><\/li>\n<li><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/public-data-missing-controls-conflicting-metadata\/\">\u516c\u5171\u6570\u636e\u7f3a\u5bf9\u7167\u3001\u5206\u7ec4\u51b2\u7a81\u6216\u5143\u6570\u636e\u4e0d\u5168\u600e\u4e48\u529e\uff1f<\/a><\/li>\n<li><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/ferroptosis-external-single-cell-experimental-validation\/\">\u94c1\u6b7b\u4ea1\u7ed3\u679c\u81f3\u5c11\u9700\u8981\u54ea\u4e9b\u5916\u90e8\u961f\u5217\u3001\u5355\u7ec6\u80de\u6216\u5b9e\u9a8c\u9a8c\u8bc1\uff1f<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u8de8\u961f\u5217\u6bd4\u8f83\u5e94\u5148\u5bf9\u9f50\u7814\u7a76\u95ee\u9898\u3001\u65b9\u5411\u548c\u7279\u5f81\u5b9a\u4e49\uff0c\u518d\u6bd4\u8f83\u5404\u81ea\u5206\u6790\u7684\u6548\u5e94\u4e0e\u4e0d\u786e\u5b9a\u6027\u3002\u4e0d\u540c\u5e73\u53f0\u7684\u539f\u59cb\u8868\u8fbe\u548c\u7b7e\u540d\u5206\u6570\u901a\u5e38\u4e0d\u80fd\u76f4\u63a5\u62fc\u5728\u540c\u4e00\u5c3a\u5ea6\u4e0a\u3002\u8de8\u7269\u79cd\u8fd8\u9700\u663e\u5f0f\u5904\u7406\u540c\u6e90\u6620\u5c04\uff1b\u6279\u6b21\u4e0e\u7ec4\u522b\u5b8c\u5168\u91cd\u5408\u65f6\uff0c\u6821\u6b63\u8f6f\u4ef6\u65e0\u6cd5\u4ece\u6570\u636e\u4e2d\u8bc6\u522b\u4e24\u8005\u5404\u81ea\u7684\u8d21\u732e\u3002<\/p>\n","protected":false},"template":"","class_list":["post-65841","ftc_question","type-ftc_question","status-publish","hentry"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question\/65841","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/ftc_question"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question\/65841\/revisions"}],"predecessor-version":[{"id":65861,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question\/65841\/revisions\/65861"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=65841"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}