{"id":65843,"date":"2026-10-10T14:00:37","date_gmt":"2026-10-10T06:00:37","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?post_type=ftc_question&p=65843"},"modified":"2026-10-10T14:00:37","modified_gmt":"2026-10-10T06:00:37","slug":"gse13195-microarray-not-rnaseq-counts","status":"publish","type":"ftc_question","link":"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/gse13195-microarray-not-rnaseq-counts\/","title":{"rendered":"GSE13195 \u4e3a\u4ec0\u4e48\u4e0d\u80fd\u6309 RNA-seq count \u6d41\u7a0b\u5904\u7406\uff1f"},"content":{"rendered":"

\u5148\u770b\u7ed3\u8bba\uff1a<\/strong>GSE13195 \u7684 GEO \u5e73\u53f0\u662f GPL5175\uff1aAffymetrix Human Exon 1.0 ST Array\uff0c\u5c5e\u4e8e\u8868\u8fbe\u82af\u7247\u3002\u82af\u7247\u4fe1\u53f7\u4e0d\u662f RNA-seq \u6d4b\u5e8f\u7247\u6bb5\u8ba1\u6570\uff0c\u4e0d\u80fd\u76f4\u63a5\u5957\u7528\u539f\u59cb count \u7684\u5dee\u5f02\u5206\u6790\u6d41\u7a0b\u3002\u590d\u73b0\u5e94\u5148\u8bc6\u522b\u4e0b\u8f7d\u6587\u4ef6\u662f\u539f\u59cb\u82af\u7247\u6570\u636e\u8fd8\u662f\u5df2\u5904\u7406\u77e9\u9635\uff0c\u518d\u6838\u5bf9\u6ce8\u91ca\u3001\u5206\u7ec4\u4e0e\u914d\u5bf9\u3002<\/p>\n

\u9002\u7528\u60c5\u51b5<\/h2>\n

\u6b63\u5728\u590d\u73b0\u5f15\u7528 GSE13195 \u7684\u6587\u7ae0\uff0c\u6216\u8005\u770b\u5230\u65e7\u6587\u5c06\u5b83\u63cf\u8ff0\u4e3a RNA-seq\uff0c\u51c6\u5907\u4e0b\u8f7d\u5e76\u5206\u6790\u8be5\u961f\u5217\u3002<\/p>\n

\u5efa\u8bae\u6309\u8fd9\u4e2a\u987a\u5e8f\u68c0\u67e5<\/h2>\n
    \n
  1. \u7528 GSE13195 \u539f\u59cb\u767b\u8bb0\u548c GPL5175 \u5e73\u53f0\u8bb0\u5f55\u786e\u8ba4\u5b9e\u9a8c\u7c7b\u578b\uff0c\u5728\u590d\u73b0\u8bf4\u660e\u4e2d\u8bb0\u5f55\u8bbf\u95ee\u65e5\u671f\u3002<\/li>\n
  2. \u9010\u9879\u5217\u51fa\u5b9e\u9645\u4e0b\u8f7d\u6587\u4ef6\u3001\u6821\u9a8c\u503c\u548c\u5904\u7406\u8bf4\u660e\uff0c\u4e0d\u51ed\u6269\u5c55\u540d\u6216\u6587\u4ef6\u4e2d\u7684\u6574\u6570\u8ba4\u5b9a\u6570\u636e\u7c7b\u578b\u3002<\/li>\n
  3. \u82e5\u83b7\u53d6\u539f\u59cb CEL \u7b49\u82af\u7247\u6587\u4ef6\uff0c\u9009\u62e9\u4e0e\u8be5\u82af\u7247\u5339\u914d\u7684\u8bfb\u53d6\u3001\u8d28\u63a7\u548c\u9884\u5904\u7406\u65b9\u6cd5\uff1b\u82e5\u83b7\u53d6\u5904\u7406\u540e\u77e9\u9635\uff0c\u5148\u6838\u5bf9\u5df2\u5b8c\u6210\u54ea\u4e9b\u6b65\u9aa4\u3002<\/li>\n
  4. \u4f7f\u7528\u5339\u914d\u7248\u672c\u7684\u63a2\u9488\u6216 transcript cluster \u6ce8\u91ca\u6620\u5c04\u5230\u57fa\u56e0\uff0c\u5e76\u4fdd\u5b58\u4e00\u5bf9\u591a\u548c\u91cd\u590d\u63a2\u9488\u7684\u5904\u7406\u89c4\u5219\u3002<\/li>\n
  5. \u6309\u6838\u5b9e\u540e\u7684\u5206\u7ec4\u3001\u914d\u5bf9\u548c\u534f\u53d8\u91cf\u5efa\u7acb\u9002\u5f53\u82af\u7247\u8868\u8fbe\u6a21\u578b\uff0c\u518d\u8003\u8651\u94c1\u6b7b\u4ea1\u76f8\u5173\u5bcc\u96c6\u6216\u8bc4\u5206\u3002<\/li>\n<\/ol>\n

    \u4ece\u4e24\u6761\u8f93\u5165\u8def\u5f84\u5f00\u59cb\u590d\u73b0<\/h2>\n
    \n\n\n\n\n\n\n\n\n
    \u62ff\u5230\u7684\u6750\u6599<\/th>\n\u9996\u5148\u505a\u4ec0\u4e48<\/th>\n\u4e0d\u80fd\u76f4\u63a5\u505a\u4ec0\u4e48<\/th>\n<\/tr>\n<\/thead>\n
    \u539f\u59cb\u82af\u7247\u6587\u4ef6<\/td>\n\u6838\u5bf9\u5e73\u53f0\u652f\u6301\u3001\u8d28\u91cf\u3001\u80cc\u666f\u4e0e\u5f52\u4e00\u5316\u6d41\u7a0b<\/td>\n\u7528 RNA-seq \u6587\u5e93\u5927\u5c0f\u65b9\u6cd5\u5904\u7406\u4fe1\u53f7<\/td>\n<\/tr>\n
    \u5904\u7406\u540e\u8868\u8fbe\u77e9\u9635<\/td>\n\u9605\u8bfb\u6570\u636e\u5904\u7406\u8bf4\u660e\u3001\u68c0\u67e5\u5c3a\u5ea6\u548c\u6ce8\u91ca<\/td>\n\u672a\u7ecf\u786e\u8ba4\u91cd\u590d\u53d6 log \u6216\u91cd\u590d\u6807\u51c6\u5316<\/td>\n<\/tr>\n
    \u53ea\u6709\u4f5c\u8005\u7ed3\u679c\u8868<\/td>\n\u68c0\u67e5\u5b8c\u6574\u6027\u5e76\u5bfb\u627e\u4e0a\u6e38\u8f93\u5165<\/td>\n\u58f0\u79f0\u5df2\u7ecf\u4ece\u539f\u59cb\u6570\u636e\u590d\u73b0<\/td>\n<\/tr>\n
    \u65e7\u6587\u622a\u56fe\u6216\u70ed\u56fe<\/td>\n\u56de\u5230 GEO \u548c\u8bba\u6587\u6750\u6599<\/td>\n\u4ece\u56fe\u5f62\u53cd\u63a8\u6837\u672c\u8eab\u4efd\u548c\u771f\u5b9e\u8868\u8fbe\u91cf<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

    \u4f9d\u636e\uff1aNCBI GEO\uff1aGSE13195 \u539f\u59cb\u767b\u8bb0\u8bb0\u5f55<\/a> \u00b7 Bioconductor\uff1alimma \u8f6f\u4ef6\u4ecb\u7ecd\u4e0e\u7528\u6237\u6307\u5357\u5165\u53e3<\/a><\/p>\n

    \u4e3a\u4ec0\u4e48\u628a\u5c0f\u6570\u56db\u820d\u4e94\u5165\u4e5f\u4e0d\u6210\u7acb<\/h2>\n

    DESeq2 \u5bf9\u8ba1\u6570\u8f93\u5165\u7684\u8981\u6c42\u6765\u81ea\u5176\u6d4b\u91cf\u548c\u7edf\u8ba1\u6a21\u578b\uff0c\u5e76\u4e0d\u662f\u7b80\u5355\u68c0\u67e5\u6587\u4ef6\u6709\u6ca1\u6709\u5c0f\u6570\u3002\u628a\u82af\u7247\u4fe1\u53f7 round() \u540e\u53d8\u6210\u6574\u6570\uff0c\u4ecd\u7136\u6ca1\u6709\u4ea7\u751f\u6d4b\u5e8f\u6587\u5e93\u548c\u8ba1\u6570\u91c7\u6837\u8fc7\u7a0b\uff0c\u4e0d\u80fd\u56e0\u6b64\u8ba9\u6d41\u7a0b\u5728\u79d1\u5b66\u4e0a\u6210\u7acb\u3002<\/p>\n

    \u82af\u7247\u6570\u636e\u53ef\u4ee5\u53c2\u4e0e\u94c1\u6b7b\u4ea1\u7814\u7a76\uff0c\u5173\u952e\u5728\u4e8e\u6309\u5176\u6d4b\u91cf\u7279\u70b9\u5904\u7406\uff0c\u518d\u89e3\u91ca\u57fa\u56e0\u96c6\u4fe1\u53f7\u3002\u9002\u5f53\u7684\u7ebf\u6027\u6a21\u578b\u8bbe\u8ba1\u662f\u540e\u7eed\u63a8\u65ad\u7684\u57fa\u7840\u3002<\/p>\n

    \u4f9d\u636e\uff1aBioconductor\uff1alimma \u8f6f\u4ef6\u4ecb\u7ecd\u4e0e\u7528\u6237\u6307\u5357\u5165\u53e3<\/a> \u00b7 DESeq2 \u5b98\u65b9\u6559\u7a0b\uff1a\u8f93\u5165\u3001\u8bbe\u8ba1\u77e9\u9635\u4e0e\u6280\u672f\u91cd\u590d<\/a><\/p>\n

    GSE13195 \u590d\u73b0\u8bb0\u5f55\u7684\u6700\u4f4e\u5b57\u6bb5<\/h2>\n

    \u672c\u9875\u786e\u8ba4\u4e86\u516c\u5f00\u767b\u8bb0\u7684\u6570\u636e\u7c7b\u578b\uff1b\u6ca1\u6709\u9010\u6837\u672c\u5b8c\u6210\u4e34\u5e8a\u5206\u7ec4\u590d\u6838\uff0c\u4e5f\u6ca1\u6709\u91cd\u65b0\u8fd0\u884c\u8be5\u961f\u5217\u3002\u4e0d\u8981\u628a\u8fd9\u4efd\u8f93\u5165\u8def\u5f84\u8bf4\u660e\u5f53\u4f5c\u5df2\u590d\u73b0\u7684\u7ed3\u679c\u62a5\u544a\u3002<\/p>\n

    accession: GSE13195\nplatform: GPL5175\nassay: Affymetrix Human Exon 1.0 ST Array\ninput_files_and_checksums: \u5f85\u586b\u5199\npreprocessing_already_done: \u5f85\u6838\u5bf9\nannotation_version: \u5f85\u586b\u5199\nsample_group_and_pairing: \u9010 GSM \u6838\u5bf9\nmodel_and_contrast: \u5f85\u586b\u5199\nreproduction_status: \u672a\u91cd\u8dd1<\/code><\/pre>\n
    \u4e0d\u80fd\u4ece\u5f53\u524d\u7ed3\u679c\u76f4\u63a5\u5f97\u51fa\u4ec0\u4e48<\/h2>\n
    \u5e73\u53f0\u786e\u8ba4\u4e0d\u7b49\u4e8e\u6837\u672c\u7b5b\u9009\u5b8c\u6210\u3002\u6837\u672c\u6570\u91cf\u3001\u53ef\u7528\u5bf9\u7167\u3001\u914d\u5bf9\u548c\u5904\u7406\u6b65\u9aa4\u5fc5\u987b\u4ee5\u5f53\u524d\u539f\u59cb\u8bb0\u5f55\u53ca\u6587\u4ef6\u4e3a\u51c6\uff0c\u4e0d\u80fd\u7167\u6284\u65e7\u6587\u4e2d\u672a\u7ecf\u6838\u5b9e\u7684\u5b57\u6bb5\u3002<\/p>\n
    \u4f9d\u636e\u4e0e\u7248\u672c<\/h2>\n