{"id":65844,"date":"2026-10-10T14:00:37","date_gmt":"2026-10-10T06:00:37","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?post_type=ftc_question&#038;p=65844"},"modified":"2026-10-10T14:00:37","modified_gmt":"2026-10-10T06:00:37","slug":"sample-run-technical-biological-replicates","status":"publish","type":"ftc_question","link":"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/sample-run-technical-biological-replicates\/","title":{"rendered":"\u4e00\u4e2a\u6837\u672c\u5bf9\u5e94\u591a\u4e2a run\uff0c\u80fd\u4e0d\u80fd\u76f4\u63a5\u5f53\u6210\u591a\u4e2a\u6837\u672c\uff1f"},"content":{"rendered":"<p class=\"ftc-answer-first\"><strong>\u5148\u770b\u7ed3\u8bba\uff1a<\/strong>\u4e0d\u80fd\u4ec5\u51ed\u591a\u4e2a SRR \u7f16\u53f7\u5c31\u589e\u52a0\u751f\u7269\u5b66\u6837\u672c\u91cf\u3002Run \u8868\u793a\u6d4b\u5e8f\u6570\u636e\u8fd0\u884c\u5355\u4f4d\uff0c\u662f\u5426\u72ec\u7acb\u91cd\u590d\u8981\u56de\u5230\u4f9b\u4f53\u3001\u53d6\u6750\u3001\u5efa\u5e93\u548c\u5b9e\u9a8c\u8bbe\u8ba1\u3002\u540c\u4e00\u6587\u5e93\u7684\u5206 lane \u6216\u8865\u6d4b\u901a\u5e38\u5c5e\u4e8e\u6280\u672f\u5c42\u9762\u7684\u91cd\u590d\uff1b\u540c\u4e00\u4f9b\u4f53\u7684\u4e0d\u540c\u65f6\u70b9\u6216\u7ec4\u7ec7\u4e5f\u6709\u76f8\u5173\u6027\uff0c\u9700\u8981\u6309\u8bbe\u8ba1\u5904\u7406\u3002<\/p>\n<h2>\u9002\u7528\u60c5\u51b5<\/h2>\n<p>\u4e0b\u8f7d GEO\/SRA \u6570\u636e\u65f6\u53d1\u73b0\u4e00\u4e2a GSM \u6216\u6837\u672c\u5173\u8054\u591a\u4e2a SRR\uff0c\u6216\u8005\u51c6\u5907\u628a\u591a\u6b21\u6d4b\u5e8f\u7ed3\u679c\u5408\u5e76\u3002<\/p>\n<h2>\u5efa\u8bae\u6309\u8fd9\u4e2a\u987a\u5e8f\u68c0\u67e5<\/h2>\n<ol>\n<li>\u5efa\u7acb\u4f9b\u4f53\u2014\u751f\u7269\u6837\u672c\u2014GSM\/BioSample\u2014Experiment\u2014Run\u2014\u6587\u5e93\u7684\u5bf9\u5e94\u8868\uff0c\u4fdd\u7559\u539f\u59cb\u8bb0\u5f55\u6765\u6e90\u3002<\/li>\n<li>\u786e\u8ba4\u591a\u4e2a run \u662f\u540c\u6587\u5e93\u8865\u6d4b\u3001\u91cd\u65b0\u5efa\u5e93\u3001\u4e0d\u540c\u7ec4\u7ec7\u8fd8\u662f\u4e0d\u540c\u65f6\u70b9\uff1b\u7f16\u53f7\u4e0d\u540c\u672c\u8eab\u4e0d\u80fd\u56de\u7b54\u72ec\u7acb\u6027\u3002<\/li>\n<li>\u4ec5\u5728\u6587\u5e93\u3001\u6d4b\u5e8f\u7b56\u7565\u3001\u914d\u5bf9\u8bfb\u6bb5\u548c\u5904\u7406\u6761\u4ef6\u517c\u5bb9\u65f6\u8003\u8651\u5408\u5e76\u6280\u672f\u6d4b\u5e8f\u6570\u636e\uff0c\u4fdd\u5b58\u6bcf\u4e2a run \u7684\u8d28\u63a7\u7ed3\u679c\u3002<\/li>\n<li>\u5df2\u7ecf\u7edf\u4e00\u8ba1\u6570\u7684\u6280\u672f\u91cd\u590d\u53ef\u6309\u9002\u7528\u6d41\u7a0b\u805a\u5408\uff1b\u4e0d\u8981\u5bf9 TPM \u6216 log \u8868\u8fbe\u503c\u673a\u68b0\u6c42\u548c\u3002<\/li>\n<li>\u7edf\u8ba1\u6a21\u578b\u4ee5\u771f\u6b63\u72ec\u7acb\u7684\u751f\u7269\u5355\u4f4d\u4e3a\u4f9d\u636e\uff1b\u540c\u4f9b\u4f53\u91cd\u590d\u6d4b\u91cf\u5e94\u91c7\u7528\u5408\u9002\u914d\u5bf9\u3001\u963b\u65ad\u6216\u76f8\u5173\u7ed3\u6784\u3002<\/li>\n<\/ol>\n<h2>\u4e00\u4e2a\u6700\u5bb9\u6613\u9ad8\u4f30\u6837\u672c\u91cf\u7684\u793a\u610f\u4f8b\u5b50<\/h2>\n<p>\u4ee5\u4e0a\u5747\u4e3a\u865a\u6784\u7f16\u53f7\u3002\u8fd9\u4e2a\u4f8b\u5b50\u6709 4 \u4e2a run\u30013 \u4e2a\u7ec4\u7ec7\u6837\u672c\u548c 2 \u4e2a\u4f9b\u4f53\uff1b\u4e09\u79cd\u6570\u91cf\u5e94\u5206\u522b\u62a5\u544a\u3002\u662f\u5426\u80fd\u7528\u4e8e\u67d0\u4e2a\u7ec4\u95f4\u68c0\u9a8c\uff0c\u8fd8\u9700\u68c0\u67e5\u5206\u7ec4\u4e0e\u72ec\u7acb\u91cd\u590d\u662f\u5426\u8db3\u591f\u3002<\/p>\n<div class=\"ftc-table-wrap\" role=\"region\" tabindex=\"0\" aria-label=\"\u4e00\u4e2a\u6700\u5bb9\u6613\u9ad8\u4f30\u6837\u672c\u91cf\u7684\u793a\u610f\u4f8b\u5b50\">\n<table>\n<thead>\n<tr>\n<th scope=\"col\">\u4f9b\u4f53\/\u751f\u7269\u6837\u672c<\/th>\n<th scope=\"col\">\u6587\u5e93<\/th>\n<th scope=\"col\">\u6d4b\u5e8f\u8fd0\u884c<\/th>\n<th scope=\"col\">\u5bf9\u91cd\u590d\u6570\u7684\u542b\u4e49<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>D01 \/ \u80bf\u7624\u7ec4\u7ec7 T01<\/td>\n<td>L01<\/td>\n<td>RUN_DEMO_1 + RUN_DEMO_2<\/td>\n<td>\u4e24\u6b21\u6d4b\u5e8f\u4ecd\u662f\u540c\u4e00\u751f\u7269\u6837\u672c<\/td>\n<\/tr>\n<tr>\n<td>D02 \/ \u80bf\u7624\u7ec4\u7ec7 T02<\/td>\n<td>L02<\/td>\n<td>RUN_DEMO_3<\/td>\n<td>\u589e\u52a0\u4e00\u4e2a\u72ec\u7acb\u4f9b\u4f53<\/td>\n<\/tr>\n<tr>\n<td>D01 \/ \u90bb\u8fd1\u7ec4\u7ec7 N01<\/td>\n<td>L03<\/td>\n<td>RUN_DEMO_4<\/td>\n<td>\u65b0\u589e\u914d\u5bf9\u7ec4\u7ec7\uff0c\u4e0d\u662f\u7b2c\u4e09\u4e2a\u72ec\u7acb\u4f9b\u4f53<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p class=\"ftc-inline-sources\">\u4f9d\u636e\uff1a<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/sra\/docs\/submitmeta\/\" target=\"_blank\" rel=\"noopener noreferrer\">NCBI SRA\uff1aStudy\u3001Sample\u3001Experiment \u4e0e Run \u5143\u6570\u636e\u7ed3\u6784<\/a><\/p>\n<h2>\u4e0d\u540c\u9636\u6bb5\u7684\u5408\u5e76\u8fb9\u754c<\/h2>\n<p>\u539f\u59cb reads \u5408\u5e76\u524d\u5148\u6838\u5bf9\u5355\u53cc\u7aef\u3001read1\/read2 \u5bf9\u5e94\u3001\u6587\u5e93\u7b56\u7565\u548c\u8d28\u91cf\uff1b\u5e94\u4fdd\u8bc1\u6240\u6709\u8bfb\u6bb5\u7684\u6765\u6e90\u4ecd\u53ef\u8ffd\u6eaf\u3002\u4e0d\u8981\u628a\u4e0d\u540c\u7ec4\u7ec7\u3001\u65f6\u95f4\u70b9\u6216\u4e0d\u540c\u6d4b\u91cf\u76ee\u6807\u4ec5\u56e0\u6765\u81ea\u540c\u4e00\u4e2a\u4eba\u800c\u5408\u5e76\u3002<\/p>\n<p>\u8ba1\u6570\u5c42\u9762\u82e5\u5df2\u786e\u8ba4\u662f\u540c\u4e00\u751f\u7269\u6837\u672c\u7684\u517c\u5bb9\u6280\u672f\u91cd\u590d\uff0c\u53ef\u4f7f\u7528\u9002\u7528\u5de5\u5177\u7684\u805a\u5408\u65b9\u6cd5\u3002DESeq2 \u6587\u6863\u63d0\u4f9b\u6280\u672f\u91cd\u590d\u5408\u5e76\u8bf4\u660e\uff0c\u4f46\u8be5\u529f\u80fd\u4e0d\u4f1a\u66ff\u4f60\u5224\u65ad\u54ea\u4e9b\u5217\u5728\u751f\u7269\u5b66\u4e0a\u5e94\u8be5\u5408\u5e76\u3002<\/p>\n<p class=\"ftc-inline-sources\">\u4f9d\u636e\uff1a<a href=\"https:\/\/bioconductor.org\/packages\/release\/bioc\/vignettes\/DESeq2\/inst\/doc\/DESeq2.html\" target=\"_blank\" rel=\"noopener noreferrer\">DESeq2 \u5b98\u65b9\u6559\u7a0b\uff1a\u8f93\u5165\u3001\u8bbe\u8ba1\u77e9\u9635\u4e0e\u6280\u672f\u91cd\u590d<\/a><\/p>\n<h2>\u9047\u5230\u5173\u7cfb\u4e0d\u6e05\u65f6\u600e\u6837\u7ee7\u7eed<\/h2>\n<p>\u5148\u5bf9\u7167\u8bba\u6587\u8865\u5145\u8868\u3001GSM \u7279\u5f81\u548c SRA \u6587\u5e93\u4fe1\u606f\uff0c\u7ed9\u6bcf\u6761\u8bb0\u5f55\u6807\u6ce8\u201c\u5df2\u786e\u8ba4\u201d\u6216\u201c\u5f85\u786e\u8ba4\u201d\u3002\u82e5\u4ecd\u4e0d\u6e05\u695a\uff0c\u5c31\u6682\u505c\u6d89\u53ca\u91cd\u590d\u6570\u7684\u7edf\u8ba1\u63a8\u65ad\uff0c\u5bfb\u627e\u4f5c\u8005\u8bf4\u660e\u6216\u53ef\u66ff\u4ee3\u961f\u5217\u3002\u4e0d\u80fd\u8ba9 AI \u6309\u7f16\u53f7\u76f8\u4f3c\u7a0b\u5ea6\u81ea\u52a8\u63a8\u65ad\u4f9b\u4f53\u8eab\u4efd\u3002<\/p>\n<h2>\u4e0d\u80fd\u4ece\u5f53\u524d\u7ed3\u679c\u76f4\u63a5\u5f97\u51fa\u4ec0\u4e48<\/h2>\n<p>GSM\u3001BioSample \u6216 SRR \u7f16\u53f7\u90fd\u4e0d\u80fd\u81ea\u52a8\u7b49\u540c\u4e8e\u72ec\u7acb\u751f\u7269\u5b66\u91cd\u590d\u3002\u6280\u672f\u6d4b\u5e8f\u66f4\u6df1\u53ef\u4ee5\u63d0\u9ad8\u6d4b\u91cf\u4fe1\u606f\uff0c\u4f46\u4e0d\u80fd\u66ff\u4ee3\u66f4\u591a\u72ec\u7acb\u4f9b\u4f53\u3002<\/p>\n<h2>\u4f9d\u636e\u4e0e\u7248\u672c<\/h2>\n<ul class=\"ftc-source-list\">\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/sra\/docs\/submitmeta\/\" target=\"_blank\" rel=\"noopener noreferrer\">NCBI SRA\uff1aStudy\u3001Sample\u3001Experiment \u4e0e Run \u5143\u6570\u636e\u7ed3\u6784<\/a><span>SRA \u5b98\u65b9\u8bf4\u660e\uff1b2026-10-10 \u6838\u5bf9<\/span><\/li>\n<li><a href=\"https:\/\/bioconductor.org\/packages\/release\/bioc\/vignettes\/DESeq2\/inst\/doc\/DESeq2.html\" target=\"_blank\" rel=\"noopener noreferrer\">DESeq2 \u5b98\u65b9\u6559\u7a0b\uff1a\u8f93\u5165\u3001\u8bbe\u8ba1\u77e9\u9635\u4e0e\u6280\u672f\u91cd\u590d<\/a><span>release \u5728\u7ebf\u6559\u7a0b\uff1b\u793a\u4f8b\u5e94\u5339\u914d\u672c\u5730\u7248\u672c<\/span><\/li>\n<\/ul>\n<p class=\"ftc-verification\">\u6700\u540e\u6838\u9a8c\uff1a2026-10-10\uff5c\u8d44\u6599\u6838\u9a8c\uff1aAI \u7f16\u5199\u4e0e\u516c\u5f00\u6765\u6e90\u6838\u5bf9\uff1b\u672a\u7ecf\u4eba\u5de5\u4e13\u5bb6\u5ba1\u9605<\/p>\n<p class=\"ftc-verification\">\u4f9d\u636e\u516c\u5f00\u6765\u6e90\u6838\u5bf9\u6982\u5ff5\u3001\u8f93\u5165\u3001\u8bc1\u636e\u8fb9\u754c\u4e0e\u7248\u672c\u53e3\u5f84\uff1b\u793a\u610f\u6570\u636e\u4e0d\u4ee3\u8868\u771f\u5b9e\u7814\u7a76\u7ed3\u679c\u3002\u4ec5\u6807\u660e\u7684\u5c0f\u578b\u4ee3\u7801\u793a\u4f8b\u7ecf\u8fc7\u8fd0\u884c\u68c0\u67e5\uff0c\u4e0d\u4ee3\u8868\u5b8c\u6574\u751f\u7269\u4fe1\u606f\u6d41\u7a0b\u6216\u539f\u8bba\u6587\u5df2\u590d\u73b0\u3002<\/p>\n<h2>\u7ee7\u7eed\u89e3\u51b3\u76f8\u5173\u95ee\u9898<\/h2>\n<ul class=\"ftc-related-reading\">\n<li><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/find-geo-ferroptosis-disease-datasets\/\">\u600e\u6837\u67e5\u627e\u9002\u5408\u67d0\u75be\u75c5\u7684 GEO \u94c1\u6b7b\u4ea1\u6570\u636e\uff1f<\/a><\/li>\n<li><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/public-data-missing-controls-conflicting-metadata\/\">\u516c\u5171\u6570\u636e\u7f3a\u5bf9\u7167\u3001\u5206\u7ec4\u51b2\u7a81\u6216\u5143\u6570\u636e\u4e0d\u5168\u600e\u4e48\u529e\uff1f<\/a><\/li>\n<li><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/ferroptosis-analysis-reproducibility-record\/\">\u600e\u6837\u8bb0\u5f55\u8f6f\u4ef6\u3001\u53c2\u6570\u548c\u73af\u5883\uff0c\u4fdd\u8bc1\u94c1\u6b7b\u4ea1\u5206\u6790\u53ef\u4ee5\u590d\u73b0\uff1f<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u4e0d\u80fd\u4ec5\u51ed\u591a\u4e2a SRR \u7f16\u53f7\u5c31\u589e\u52a0\u751f\u7269\u5b66\u6837\u672c\u91cf\u3002Run \u8868\u793a\u6d4b\u5e8f\u6570\u636e\u8fd0\u884c\u5355\u4f4d\uff0c\u662f\u5426\u72ec\u7acb\u91cd\u590d\u8981\u56de\u5230\u4f9b\u4f53\u3001\u53d6\u6750\u3001\u5efa\u5e93\u548c\u5b9e\u9a8c\u8bbe\u8ba1\u3002\u540c\u4e00\u6587\u5e93\u7684\u5206 lane \u6216\u8865\u6d4b\u901a\u5e38\u5c5e\u4e8e\u6280\u672f\u5c42\u9762\u7684\u91cd\u590d\uff1b\u540c\u4e00\u4f9b\u4f53\u7684\u4e0d\u540c\u65f6\u70b9\u6216\u7ec4\u7ec7\u4e5f\u6709\u76f8\u5173\u6027\uff0c\u9700\u8981\u6309\u8bbe\u8ba1\u5904\u7406\u3002<\/p>\n","protected":false},"template":"","class_list":["post-65844","ftc_question","type-ftc_question","status-publish","hentry"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question\/65844","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/ftc_question"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question\/65844\/revisions"}],"predecessor-version":[{"id":65864,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question\/65844\/revisions\/65864"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=65844"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}