{"id":65850,"date":"2026-10-10T14:00:37","date_gmt":"2026-10-10T06:00:37","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?post_type=ftc_question&#038;p=65850"},"modified":"2026-10-10T14:00:37","modified_gmt":"2026-10-10T06:00:37","slug":"ferroptosis-external-single-cell-experimental-validation","status":"publish","type":"ftc_question","link":"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/ferroptosis-external-single-cell-experimental-validation\/","title":{"rendered":"\u94c1\u6b7b\u4ea1\u7ed3\u679c\u81f3\u5c11\u9700\u8981\u54ea\u4e9b\u5916\u90e8\u961f\u5217\u3001\u5355\u7ec6\u80de\u6216\u5b9e\u9a8c\u9a8c\u8bc1\uff1f"},"content":{"rendered":"<p class=\"ftc-answer-first\"><strong>\u5148\u770b\u7ed3\u8bba\uff1a<\/strong>\u6ca1\u6709\u9002\u7528\u4e8e\u6240\u6709\u8bfe\u9898\u7684\u56fa\u5b9a\u201c\u6700\u4f4e\u9a8c\u8bc1\u5957\u9910\u201d\u3002\u5148\u5217\u51fa\u6587\u7ae0\u7684\u4e3b\u8981\u4e3b\u5f20\uff0c\u518d\u7ed9\u6bcf\u6761\u4e3b\u5f20\u5339\u914d\u80fd\u68c0\u9a8c\u5b83\u7684\u72ec\u7acb\u8bc1\u636e\uff1a\u5916\u90e8\u961f\u5217\u9a8c\u8bc1\u53ef\u63a8\u5e7f\u6027\uff0c\u5355\u7ec6\u80de\u5e2e\u52a9\u5b9a\u4f4d\u6765\u6e90\uff0c\u529f\u80fd\u5b9e\u9a8c\u68c0\u9a8c\u673a\u5236\u3002\u4e09\u4e2a\u65b9\u5411\u5404\u6709\u7528\u9014\uff0c\u5f7c\u6b64\u4e0d\u80fd\u7b80\u5355\u66ff\u4ee3\u3002<\/p>\n<h2>\u9002\u7528\u60c5\u51b5<\/h2>\n<p>\u5df2\u7ecf\u5f97\u5230\u94c1\u6b7b\u4ea1\u76f8\u5173\u7ed3\u679c\uff0c\u5e0c\u671b\u51b3\u5b9a\u4e0b\u4e00\u7b14\u5206\u6790\u6216\u5b9e\u9a8c\u8d44\u6e90\u5e94\u8be5\u6295\u5411\u54ea\u91cc\uff0c\u6216\u5224\u65ad\u76ee\u524d\u8bc1\u636e\u662f\u5426\u5b8c\u6574\u3002<\/p>\n<h2>\u5efa\u8bae\u6309\u8fd9\u4e2a\u987a\u5e8f\u68c0\u67e5<\/h2>\n<ol>\n<li>\u9010\u6761\u5217\u51fa\u4e3b\u5f20\uff1a\u8868\u8fbe\u5173\u8054\u3001\u7ec6\u80de\u6765\u6e90\u3001\u9884\u6d4b\u6027\u80fd\u3001\u673a\u5236\u4f9d\u8d56\uff0c\u907f\u514d\u628a\u5b83\u4eec\u6df7\u5199\u4e3a\u4e00\u4e2a\u7ed3\u8bba\u3002<\/li>\n<li>\u4e3a\u6bcf\u6761\u4e3b\u5f20\u6307\u51fa\u6700\u5173\u952e\u7684\u66ff\u4ee3\u89e3\u91ca\uff0c\u4f8b\u5982\u6279\u6b21\u3001\u7ec6\u80de\u7ec4\u6210\u3001\u6570\u636e\u6cc4\u6f0f\u6216\u975e\u7279\u5f02\u635f\u4f24\u3002<\/li>\n<li>\u9009\u62e9\u53ef\u4ee5\u533a\u5206\u8be5\u89e3\u91ca\u7684\u9a8c\u8bc1\u6750\u6599\uff0c\u660e\u786e\u72ec\u7acb\u6027\u3001\u6d4b\u91cf\u5c42\u7ea7\u548c\u9884\u8bbe\u6210\u529f\u6807\u51c6\u3002<\/li>\n<li>\u5728\u9a8c\u8bc1\u5f00\u59cb\u524d\u56fa\u5b9a\u6a21\u578b\u3001\u57fa\u56e0\u96c6\u548c\u4e3b\u8981\u8bc4\u4ef7\u65b9\u5f0f\uff0c\u4fdd\u7559\u4e0d\u4e00\u81f4\u7ed3\u679c\u53ca\u5904\u7406\u8fc7\u7a0b\u3002<\/li>\n<li>\u68c0\u67e5\u8bc1\u636e\u662f\u5426\u6765\u81ea\u540c\u4e00\u5e95\u5c42\u6837\u672c\u6216\u540c\u4e00\u5206\u6790\u4fe1\u606f\uff1b\u9a8c\u8bc1\u5b8c\u6210\u540e\u91cd\u65b0\u754c\u5b9a\u7ed3\u8bba\u8303\u56f4\u3002<\/li>\n<\/ol>\n<h2>\u628a\u9a8c\u8bc1\u9700\u6c42\u5bf9\u5e94\u5230\u4e3b\u5f20<\/h2>\n<div class=\"ftc-table-wrap\" role=\"region\" tabindex=\"0\" aria-label=\"\u628a\u9a8c\u8bc1\u9700\u6c42\u5bf9\u5e94\u5230\u4e3b\u5f20\">\n<table>\n<thead>\n<tr>\n<th scope=\"col\">\u4e3b\u8981\u4e3b\u5f20<\/th>\n<th scope=\"col\">\u4f18\u5148\u9a8c\u8bc1\u5185\u5bb9<\/th>\n<th scope=\"col\">\u4e0d\u80fd\u66ff\u4ee3\u5b83\u7684\u6750\u6599<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\u8868\u8fbe\u5173\u8054\u53ef\u63a8\u5e7f<\/td>\n<td>\u72ec\u7acb\u961f\u5217\u4e2d\u7684\u65b9\u5411\u4e0e\u6548\u5e94<\/td>\n<td>\u540c\u4e00\u961f\u5217\u91cd\u590d\u6362\u7b97\u6cd5<\/td>\n<\/tr>\n<tr>\n<td>\u4fe1\u53f7\u6765\u81ea\u67d0\u7ec6\u80de\u7c7b\u578b<\/td>\n<td>\u5408\u9002\u5355\u7ec6\u80de\u3001\u7a7a\u95f4\u6216\u7ec6\u80de\u7279\u5f02\u68c0\u6d4b<\/td>\n<td>\u4ec5\u6709 bulk \u76f8\u5173\u6027<\/td>\n<\/tr>\n<tr>\n<td>\u6a21\u578b\u9884\u6d4b\u53ef\u7528<\/td>\n<td>\u51bb\u7ed3\u6a21\u578b\u7684\u5916\u90e8\u6027\u80fd\u3001\u6821\u51c6\u548c\u9002\u7528\u6027<\/td>\n<td>\u91cd\u65b0\u8bad\u7ec3\u540e\u4ecd\u53eb\u5916\u90e8\u9a8c\u8bc1<\/td>\n<\/tr>\n<tr>\n<td>\u94c1\u6b7b\u4ea1\u53c2\u4e0e\u673a\u5236<\/td>\n<td>\u6b7b\u4ea1\u3001\u8102\u8d28\u6c27\u5316\u4e0e\u673a\u5236\u76f8\u5173\u5e72\u9884\u8bc1\u636e<\/td>\n<td>\u8868\u8fbe\u7b7e\u540d\u6216\u5355\u4e2a marker<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p class=\"ftc-inline-sources\">\u4f9d\u636e\uff1a<a href=\"https:\/\/www.tripod-statement.org\/resources\/\" target=\"_blank\" rel=\"noopener noreferrer\">TRIPOD \u5b98\u65b9\u8d44\u6e90\uff1a\u9884\u6d4b\u6a21\u578b\u5f00\u53d1\u4e0e\u9a8c\u8bc1\u6e05\u5355<\/a> \u00b7 <a href=\"https:\/\/tisch.compbio.cn\/home\/\" target=\"_blank\" rel=\"noopener noreferrer\">TISCH2\uff1a\u80bf\u7624\u5fae\u73af\u5883\u5355\u7ec6\u80de\u6570\u636e\u5e93<\/a> \u00b7 <a href=\"https:\/\/www.nature.com\/articles\/s41418-017-0012-4\" target=\"_blank\" rel=\"noopener noreferrer\">NCCD\uff1a\u7ec6\u80de\u6b7b\u4ea1\u7684\u673a\u5236\u5b9a\u4e49\u4e0e\u8bc1\u636e\u89e3\u91ca<\/a><\/p>\n<h2>\u600e\u6837\u8bc6\u522b\u201c\u770b\u4f3c\u72ec\u7acb\u201d\u7684\u9a8c\u8bc1<\/h2>\n<p>\u540c\u4e00 GEO \u961f\u5217\u5728\u53e6\u4e00\u6570\u636e\u5e93\u5c55\u793a\uff0c\u4e0d\u662f\u65b0\u7684\u6837\u672c\uff1b\u628a\u540c\u4e00\u4f9b\u4f53\u7ec6\u80de\u968f\u673a\u5206\u6210\u8bad\u7ec3\u548c\u6d4b\u8bd5\uff0c\u4e5f\u4e0d\u80fd\u68c0\u9a8c\u5bf9\u65b0\u4f9b\u4f53\u7684\u6cdb\u5316\uff1b\u5728\u9a8c\u8bc1\u961f\u5217\u4e2d\u91cd\u65b0\u7b5b\u9009\u6700\u663e\u8457\u57fa\u56e0\uff0c\u518d\u62a5\u544a\u6027\u80fd\uff0c\u5df2\u7ecf\u6539\u53d8\u4e86\u88ab\u9a8c\u8bc1\u7684\u65b9\u6848\u3002<\/p>\n<p>\u793a\u610f\u60c5\u5883\uff1a\u8bad\u7ec3\u51fa\u4e00\u4e2a\u98ce\u9669\u8bc4\u5206\u540e\uff0c\u5728\u65b0\u961f\u5217\u6309\u539f\u516c\u5f0f\u7b97\u5206\uff0c\u5e76\u4f7f\u7528\u9884\u8bbe\u8bc4\u4f30\u65b9\u6cd5\uff0c\u624d\u662f\u5728\u8bc4\u4f30\u539f\u65b9\u6848\u7684\u5916\u90e8\u8868\u73b0\u3002\u82e5\u5e73\u53f0\u4e0d\u540c\u5bfc\u81f4\u67d0\u4e9b\u57fa\u56e0\u4e0d\u53ef\u6d4b\uff0c\u5e94\u62a5\u544a\u517c\u5bb9\u6027\u95ee\u9898\uff0c\u4e0d\u80fd\u9759\u9ed8\u5220\u6389\u540e\u4ecd\u79f0\u540c\u4e00\u4e2a\u6a21\u578b\u3002<\/p>\n<h2>\u9884\u7b97\u6709\u9650\u65f6\u6309\u7f3a\u53e3\u6392\u5e8f<\/h2>\n<p>\u5148\u627e\u6700\u53ef\u80fd\u63a8\u7ffb\u4e3b\u8981\u7ed3\u8bba\u7684\u7f3a\u53e3\u3002\u4f8b\u5982 bulk \u4fe1\u53f7\u53ef\u80fd\u5b8c\u5168\u6765\u81ea\u7ec6\u80de\u6bd4\u4f8b\u65f6\uff0c\u7ec6\u80de\u5b9a\u4f4d\u7684\u4ef7\u503c\u53ef\u80fd\u9ad8\u4e8e\u518d\u505a\u4e00\u5957\u540c\u7c7b\u76f8\u5173\u56fe\uff1b\u82e5\u6587\u7ae0\u6838\u5fc3\u662f\u673a\u5236\uff0c\u5219\u66f4\u591a\u89c2\u5bdf\u6027\u961f\u5217\u4ecd\u65e0\u6cd5\u66ff\u4ee3\u6070\u5f53\u5e72\u9884\u3002<\/p>\n<p>\u53ef\u5efa\u7acb\u56db\u5217\u6e05\u5355\uff1a\u4e3b\u5f20\u3001\u5f53\u524d\u8bc1\u636e\u3001\u6700\u5927\u7f3a\u53e3\u3001\u4e0b\u4e00\u9879\u9a8c\u8bc1\u3002\u6bcf\u9879\u660e\u786e\u8f93\u5165\u3001\u72ec\u7acb\u5355\u4f4d\u3001\u9884\u671f\u8f93\u51fa\u548c\u65e0\u6cd5\u5224\u5b9a\u7684\u60c5\u5f62\uff0c\u518d\u5b89\u6392\u8d44\u6e90\u3002\u6ca1\u6709\u8db3\u591f\u9a8c\u8bc1\u6761\u4ef6\u65f6\uff0c\u6536\u7d27\u4e3b\u5f20\u4e5f\u662f\u5408\u7406\u7ed3\u679c\u3002<\/p>\n<h2>\u53ef\u76f4\u63a5\u4f7f\u7528\u7684\u8bb0\u5f55\u6a21\u677f<\/h2>\n<ul>\n<li><a download href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/plugins\/biocloud-ferroptosis-center\/downloads\/validation-checklist.txt\">\u4e0b\u8f7d\u4e3b\u5f20\u4e0e\u9a8c\u8bc1\u7f3a\u53e3\u6e05\u5355<\/a>\uff08TXT\uff0c\u65e0\u9700\u7559\u8054\u7cfb\u65b9\u5f0f\uff09<\/li>\n<\/ul>\n<h2>\u4e0d\u80fd\u4ece\u5f53\u524d\u7ed3\u679c\u76f4\u63a5\u5f97\u51fa\u4ec0\u4e48<\/h2>\n<p>\u4e0d\u5b58\u5728\u201c\u52a0\u4e00\u4e2a\u5916\u90e8\u961f\u5217\u201d\u6216\u201c\u505a\u4e09\u9879\u5b9e\u9a8c\u201d\u5373\u53ef\u4fdd\u8bc1\u7ed3\u8bba\u6210\u7acb\u7684\u7edf\u4e00\u95e8\u69db\u3002\u5177\u4f53\u6837\u672c\u91cf\u3001\u5bf9\u7167\u4e0e\u7edf\u8ba1\u8bbe\u8ba1\u53d6\u51b3\u4e8e\u6548\u5e94\u3001\u53d8\u5f02\u548c\u76ee\u6807\uff0c\u4e0d\u80fd\u5728\u7f3a\u5c11\u8fd9\u4e9b\u4fe1\u606f\u65f6\u4f5c\u56fa\u5b9a\u627f\u8bfa\u3002<\/p>\n<h2>\u4f9d\u636e\u4e0e\u7248\u672c<\/h2>\n<ul class=\"ftc-source-list\">\n<li><a href=\"https:\/\/www.tripod-statement.org\/resources\/\" target=\"_blank\" rel=\"noopener noreferrer\">TRIPOD \u5b98\u65b9\u8d44\u6e90\uff1a\u9884\u6d4b\u6a21\u578b\u5f00\u53d1\u4e0e\u9a8c\u8bc1\u6e05\u5355<\/a><span>2015 \u57fa\u7840\u8d44\u6599\u4e0e\u52d8\u8bef\uff1b\u73b0\u884c\u66f4\u65b0\u53e6\u89c1 TRIPOD+AI<\/span><\/li>\n<li><a href=\"https:\/\/tisch.compbio.cn\/home\/\" target=\"_blank\" rel=\"noopener noreferrer\">TISCH2\uff1a\u80bf\u7624\u5fae\u73af\u5883\u5355\u7ec6\u80de\u6570\u636e\u5e93<\/a><span>TISCH2\uff1b2026-10-10 \u6838\u5bf9<\/span><\/li>\n<li><a href=\"https:\/\/www.nature.com\/articles\/s41418-017-0012-4\" target=\"_blank\" rel=\"noopener noreferrer\">NCCD\uff1a\u7ec6\u80de\u6b7b\u4ea1\u7684\u673a\u5236\u5b9a\u4e49\u4e0e\u8bc1\u636e\u89e3\u91ca<\/a><span>Cell Death &amp; Differentiation\uff0c2018\uff1b\u673a\u5236\u5b9a\u4e49\u53c2\u8003<\/span><\/li>\n<\/ul>\n<p class=\"ftc-verification\">\u6700\u540e\u6838\u9a8c\uff1a2026-10-10\uff5c\u8d44\u6599\u6838\u9a8c\uff1aAI \u7f16\u5199\u4e0e\u516c\u5f00\u6765\u6e90\u6838\u5bf9\uff1b\u672a\u7ecf\u4eba\u5de5\u4e13\u5bb6\u5ba1\u9605<\/p>\n<p class=\"ftc-verification\">\u4f9d\u636e\u516c\u5f00\u6765\u6e90\u6838\u5bf9\u6982\u5ff5\u3001\u8f93\u5165\u3001\u8bc1\u636e\u8fb9\u754c\u4e0e\u7248\u672c\u53e3\u5f84\uff1b\u793a\u610f\u6570\u636e\u4e0d\u4ee3\u8868\u771f\u5b9e\u7814\u7a76\u7ed3\u679c\u3002\u4ec5\u6807\u660e\u7684\u5c0f\u578b\u4ee3\u7801\u793a\u4f8b\u7ecf\u8fc7\u8fd0\u884c\u68c0\u67e5\uff0c\u4e0d\u4ee3\u8868\u5b8c\u6574\u751f\u7269\u4fe1\u606f\u6d41\u7a0b\u6216\u539f\u8bba\u6587\u5df2\u590d\u73b0\u3002<\/p>\n<h2>\u7ee7\u7eed\u89e3\u51b3\u76f8\u5173\u95ee\u9898<\/h2>\n<ul class=\"ftc-related-reading\">\n<li><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/bulk-single-cell-ferroptosis-evidence-chain\/\">bulk RNA-seq \u4e0e\u5355\u7ec6\u80de\u5982\u4f55\u7ec4\u6210\u94c1\u6b7b\u4ea1\u8bc1\u636e\u94fe\uff1f<\/a><\/li>\n<li><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/ferroptosis-model-overfitting-validation\/\">\u7528\u94c1\u6b7b\u4ea1\u57fa\u56e0\u505a WGCNA\u3001\u673a\u5668\u5b66\u4e60\u6216\u9884\u540e\u6a21\u578b\uff0c\u600e\u6837\u907f\u514d\u8fc7\u62df\u5408\uff1f<\/a><\/li>\n<li><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/reviewer-asks-ferroptosis-validation\/\">\u5ba1\u7a3f\u4eba\u8981\u6c42\u8865\u94c1\u6b7b\u4ea1\u9a8c\u8bc1\uff0c\u6700\u4f4e\u5e94\u5148\u8865\u54ea\u4e9b\u8bc1\u636e\uff1f<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u6ca1\u6709\u9002\u7528\u4e8e\u6240\u6709\u8bfe\u9898\u7684\u56fa\u5b9a\u201c\u6700\u4f4e\u9a8c\u8bc1\u5957\u9910\u201d\u3002\u5148\u5217\u51fa\u6587\u7ae0\u7684\u4e3b\u8981\u4e3b\u5f20\uff0c\u518d\u7ed9\u6bcf\u6761\u4e3b\u5f20\u5339\u914d\u80fd\u68c0\u9a8c\u5b83\u7684\u72ec\u7acb\u8bc1\u636e\uff1a\u5916\u90e8\u961f\u5217\u9a8c\u8bc1\u53ef\u63a8\u5e7f\u6027\uff0c\u5355\u7ec6\u80de\u5e2e\u52a9\u5b9a\u4f4d\u6765\u6e90\uff0c\u529f\u80fd\u5b9e\u9a8c\u68c0\u9a8c\u673a\u5236\u3002\u4e09\u4e2a\u65b9\u5411\u5404\u6709\u7528\u9014\uff0c\u5f7c\u6b64\u4e0d\u80fd\u7b80\u5355\u66ff\u4ee3\u3002<\/p>\n","protected":false},"template":"","class_list":["post-65850","ftc_question","type-ftc_question","status-publish","hentry"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question\/65850","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/ftc_question"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question\/65850\/revisions"}],"predecessor-version":[{"id":65870,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/ftc_question\/65850\/revisions\/65870"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=65850"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}