{"id":65851,"date":"2026-10-10T14:00:37","date_gmt":"2026-10-10T06:00:37","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?post_type=ftc_question&p=65851"},"modified":"2026-10-10T14:00:37","modified_gmt":"2026-10-10T06:00:37","slug":"cellchat-scenic-trajectory-resource-planning","status":"publish","type":"ftc_question","link":"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/cellchat-scenic-trajectory-resource-planning\/","title":{"rendered":"CellChat\u3001SCENIC\u3001\u62df\u65f6\u5e8f\u4e0e\u94c1\u6b7b\u4ea1\u8054\u5408\u5206\u6790\u9700\u8981\u4ec0\u4e48\u8d44\u6e90\uff1f"},"content":{"rendered":"

\u5148\u770b\u7ed3\u8bba\uff1a<\/strong>\u628a\u6d41\u7a0b\u62c6\u5f00\u6d4b\u91cf\uff0c\u624d\u80fd\u4f30\u7b97\u8d44\u6e90\u3002CellChat \u7684\u901a\u4fe1\u63a8\u65ad\u3001SCENIC \u7684\u7f51\u7edc\u4e0e motif \u6b65\u9aa4\u3001\u62df\u65f6\u5e8f\u7684\u56fe\u5b66\u4e60\u5404\u6709\u4e0d\u540c\u74f6\u9888\uff1b\u8bc4\u5206\u6b65\u9aa4\u672c\u8eab\u5f80\u5f80\u4e0d\u662f\u5168\u90e8\u6210\u672c\u3002\u7ec6\u80de\u6570\u3001\u57fa\u56e0\u6570\u3001\u6570\u636e\u5e93\u3001\u5e76\u884c\u6570\u548c\u5bf9\u8c61\u590d\u5236\u5171\u540c\u51b3\u5b9a\u5cf0\u503c\uff0c\u4e0d\u5b9c\u76f4\u63a5\u5957\u7528\u4e00\u4e2a\u5185\u5b58\u6570\u5b57\u3002<\/p>\n

\u9002\u7528\u60c5\u51b5<\/h2>\n

\u5df2\u7ecf\u6709\u5355\u7ec6\u80de\u5bf9\u8c61\uff0c\u51c6\u5907\u628a\u94c1\u6b7b\u4ea1\u7b7e\u540d\u4e0e\u901a\u4fe1\u7f51\u7edc\u3001\u8f6c\u5f55\u8c03\u63a7\u6216\u8f68\u8ff9\u5206\u6790\u7ed3\u5408\uff0c\u5e0c\u671b\u9009\u62e9\u8fd0\u884c\u73af\u5883\u3002<\/p>\n

\u5efa\u8bae\u6309\u8fd9\u4e2a\u987a\u5e8f\u68c0\u67e5<\/h2>\n
    \n
  1. \u5148\u786e\u8ba4\u6bcf\u4e2a\u5de5\u5177\u56de\u7b54\u7684\u79d1\u5b66\u95ee\u9898\uff0c\u53ea\u4fdd\u7559\u4e0e\u7814\u7a76\u5047\u8bbe\u76f8\u5173\u7684\u6b65\u9aa4\u3001\u7ec6\u80de\u7fa4\u548c\u6bd4\u8f83\u3002<\/li>\n
  2. \u8bb0\u5f55\u7ec6\u80de\u4e0e\u57fa\u56e0\u6570\u91cf\u3001\u7a00\u758f\u6027\u3001\u8f93\u5165\u5bf9\u8c61\u4f53\u79ef\u3001\u7269\u79cd\u3001\u6570\u636e\u5e93\u548c\u8f6f\u4ef6\u7248\u672c\u3002<\/li>\n
  3. \u5bf9\u6bcf\u4e00\u6b65\u5355\u72ec\u8bd5\u8dd1\u4ee3\u8868\u6027\u5b50\u96c6\uff0c\u4fdd\u5b58\u8017\u65f6\u3001\u5cf0\u503c\u5185\u5b58\u3001\u8fdb\u7a0b\u6570\u3001\u78c1\u76d8\u4e0e\u65e5\u5fd7\u3002<\/li>\n
  4. \u4fdd\u6301\u5b50\u96c6\u7684\u4f9b\u4f53\u548c\u7ec6\u80de\u7c7b\u578b\u7ed3\u6784\uff0c\u907f\u514d\u968f\u673a\u62bd\u6837\u6f0f\u6389\u7a00\u6709\u7fa4\uff1b\u8bd5\u8dd1\u7ed3\u679c\u4e0d\u8981\u7b80\u5355\u6309\u7ec6\u80de\u6570\u7ebf\u6027\u653e\u5927\u3002<\/li>\n
  5. \u6839\u636e\u4e3b\u74f6\u9888\u5206\u914d CPU\u3001\u5185\u5b58\u4e0e\u5b58\u50a8\uff0c\u5e76\u5728\u6269\u5927\u5e76\u884c\u6216\u5168\u91cf\u8fd0\u884c\u524d\u68c0\u67e5\u8d44\u6e90\u4f59\u91cf\u4e0e\u53ef\u6062\u590d\u70b9\u3002<\/li>\n<\/ol>\n

    \u4e0d\u540c\u5de5\u5177\u5206\u522b\u6d4b\u4ec0\u4e48<\/h2>\n
    \n\n\n\n\n\n\n\n\n
    \u6b65\u9aa4<\/th>\n\u4f18\u5148\u8bb0\u5f55<\/th>\n\u8d44\u6e90\u98ce\u9669<\/th>\n<\/tr>\n<\/thead>\n
    CellChat<\/td>\n\u7ec6\u80de\u7fa4\u89c4\u6a21\u3001\u6570\u636e\u5e93\u3001\u7f6e\u6362\u8bbe\u7f6e\u548c\u5bf9\u8c61\u7248\u672c<\/td>\n\u7fa4\u4f53\u6570\u91cf\u3001\u8868\u8fbe\u77e9\u9635\u548c\u4e2d\u95f4\u5bf9\u8c61<\/td>\n<\/tr>\n
    pySCENIC<\/td>\nGRN \u8f93\u5165\u3001worker \u6570\u3001motif \u6570\u636e\u5e93\u4e0e\u7269\u79cd<\/td>\n\u5e76\u884c\u590d\u5236\u3001\u6570\u636e\u5e93\u8bfb\u53d6\u548c\u4e34\u65f6\u7ed3\u679c<\/td>\n<\/tr>\n
    Monocle 3 \u7b49\u8f68\u8ff9<\/td>\n\u6240\u7528\u7ec6\u80de\u7fa4\u3001\u964d\u7ef4\u3001\u56fe\u5b66\u4e60\u4e0e\u6839\u8282\u70b9<\/td>\n\u5168\u91cf\u56fe\u5b66\u4e60\u3001\u91cd\u590d\u8fd0\u884c\u53ca\u5bf9\u8c61\u4fdd\u5b58<\/td>\n<\/tr>\n
    \u94c1\u6b7b\u4ea1\u8bc4\u5206<\/td>\n\u65b9\u6cd5\u3001\u57fa\u56e0\u96c6\u547d\u4e2d\u7387\u3001assay\/layer<\/td>\n\u610f\u5916\u7a20\u5bc6\u5316\u6216\u590d\u5236\u6574\u4e2a\u5bf9\u8c61<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

    \u4f9d\u636e\uff1aCellChat \u5b98\u65b9\u4ee3\u7801\u3001\u7248\u672c\u8bf4\u660e\u4e0e\u6559\u7a0b<\/a> \u00b7 pySCENIC \u5b98\u65b9\u6587\u6863<\/a> \u00b7 Monocle 3\uff1a\u8f68\u8ff9\u4e0e\u6839\u8282\u70b9\u9009\u62e9<\/a> \u00b7 Seurat v5\uff1aassay\u3001layer \u4e0e\u6570\u636e\u8bbf\u95ee<\/a><\/p>\n

    \u7a20\u5bc6\u77e9\u9635\u7684\u4e0b\u9650\u4f30\u7b97\u793a\u4f8b<\/h2>\n

    \u8fd9\u4e2a\u4eba\u5de5\u89c4\u6a21\u793a\u4f8b\u53ea\u8ba1\u7b97\u4e00\u4e2a 20,000 \u00d7 100,000 \u7684\u53cc\u7cbe\u5ea6\u7a20\u5bc6\u77e9\u9635\uff0c\u7ea6 14.9 GiB\uff1b\u5b83\u4e0d\u662f\u670d\u52a1\u5668\u914d\u7f6e\u5efa\u8bae\u3002\u591a\u4e2a\u5c42\u3001\u590d\u5236\u3001\u5de5\u4f5c\u8fdb\u7a0b\u548c\u7b97\u6cd5\u4e2d\u95f4\u91cf\u4f1a\u589e\u52a0\u5360\u7528\uff0c\u7a00\u758f\u6216\u78c1\u76d8\u5b58\u50a8\u5219\u53ef\u80fd\u964d\u4f4e\u5e38\u9a7b\u5185\u5b58\u3002<\/p>\n

    \u56e0\u6b64\u5bf9\u8c61\u6587\u4ef6\u5927\u5c0f\u3001\u4e00\u4e2a\u77e9\u9635\u7684\u7406\u8bba\u5927\u5c0f\u4e0e\u6574\u4e2a\u4efb\u52a1\u5cf0\u503c\u5e94\u5206\u522b\u8bb0\u5f55\u3002\u7ed9 4 \u4e2a worker \u4e0d\u80fd\u5047\u8bbe\u53ea\u589e\u52a0 CPU\uff1b\u5b83\u4eec\u53ef\u80fd\u5404\u81ea\u6301\u6709\u8f93\u5165\u6216\u4e2d\u95f4\u7ed3\u679c\u3002<\/p>\n

    # \u7406\u8bba\u5b57\u8282\u6570\uff0c\u53ea\u8ba1\u7b97\u4e00\u4e2a double \u77e9\u9635\uff0c\u4e0d\u662f\u4efb\u52a1\u5cf0\u503c\nn_genes <- 20000\nn_cells <- 100000\ndense_gib <- n_genes * n_cells * 8 \/ 1024^3\nstopifnot(abs(dense_gib - 14.9011611938) < 1e-6)\nprint(dense_gib)<\/code><\/pre>\n
    \u5148\u6838\u5bf9\u7248\u672c\uff0c\u518d\u6bd4\u8f83\u8bd5\u8dd1\u7ed3\u679c<\/h2>\n
    CellChat \u5b98\u65b9\u4ed3\u5e93\u533a\u5206 v1\/v2 \u4e0e Spatial CellChat v3 \u7684\u5165\u53e3\uff1bpySCENIC \u6587\u6863\u4e5f\u5217\u6709\u6570\u636e\u5e93\u683c\u5f0f\u4e0e\u4f9d\u8d56\u53d8\u5316\u3002\u590d\u73b0\u65e7\u7ed3\u679c\u5e94\u4fdd\u5b58\u51c6\u786e\u7248\u672c\uff0c\u4e0d\u80fd\u628a\u4e0d\u540c\u7248\u672c\u6216\u4e0d\u540c\u4efb\u52a1\u7684\u8d44\u6e90\u7ed3\u679c\u76f4\u63a5\u6bd4\u8f83\u3002<\/p>\n
    \u62df\u65f6\u5e8f\u7684\u6839\u8282\u70b9\u9700\u8981\u751f\u7269\u5b66\u4f9d\u636e\uff0c\u8f68\u8ff9\u65b9\u5411\u4e0d\u662f\u5b9e\u9645\u91c7\u6837\u65f6\u95f4\u7684\u81ea\u52a8\u8bc1\u660e\u3002\u7b97\u529b\u8db3\u591f\u53ea\u80fd\u4fdd\u8bc1\u67d0\u4e9b\u8ba1\u7b97\u6709\u673a\u4f1a\u5b8c\u6210\uff0c\u4e0d\u80fd\u4fee\u590d\u7814\u7a76\u95ee\u9898\u3001\u6ce8\u91ca\u6216\u65b9\u6cd5\u9009\u62e9\u7684\u9519\u8bef\u3002<\/p>\n
    \u4f9d\u636e\uff1aCellChat \u5b98\u65b9\u4ee3\u7801\u3001\u7248\u672c\u8bf4\u660e\u4e0e\u6559\u7a0b<\/a> \u00b7 pySCENIC \u5b98\u65b9\u6587\u6863<\/a> \u00b7 Monocle 3\uff1a\u8f68\u8ff9\u4e0e\u6839\u8282\u70b9\u9009\u62e9<\/a><\/p>\n
    \u53ef\u76f4\u63a5\u4f7f\u7528\u7684\u8bb0\u5f55\u6a21\u677f<\/h2>\n