{"id":65854,"date":"2026-10-10T14:00:37","date_gmt":"2026-10-10T06:00:37","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?post_type=ftc_question&p=65854"},"modified":"2026-10-10T14:00:37","modified_gmt":"2026-10-10T06:00:37","slug":"ferroptosis-analysis-reproducibility-record","status":"publish","type":"ftc_question","link":"https:\/\/www.biocloudservice.com\/wordpress\/ferroptosis\/questions\/ferroptosis-analysis-reproducibility-record\/","title":{"rendered":"\u600e\u6837\u8bb0\u5f55\u8f6f\u4ef6\u3001\u53c2\u6570\u548c\u73af\u5883\uff0c\u4fdd\u8bc1\u94c1\u6b7b\u4ea1\u5206\u6790\u53ef\u4ee5\u590d\u73b0\uff1f"},"content":{"rendered":"

\u5148\u770b\u7ed3\u8bba\uff1a<\/strong>\u53ef\u590d\u73b0\u9700\u8981\u540c\u65f6\u4fdd\u5b58\u8f93\u5165\u3001\u6837\u672c\u4e0e\u57fa\u56e0\u96c6\u53e3\u5f84\u3001\u4ee3\u7801\u3001\u53c2\u6570\u3001\u73af\u5883\u548c\u7ed3\u679c\u9a8c\u6536\u6807\u51c6\u3002\u53ea\u6709\u811a\u672c\u6216 sessionInfo \u90fd\u4e0d\u591f\u3002\u9501\u5b9a\u6587\u4ef6\u4e0e\u73af\u5883\u540e\uff0c\u8fd8\u5e94\u4ece\u5e72\u51c0\u4f1a\u8bdd\u6309\u987a\u5e8f\u8fd0\u884c\u4e00\u4e2a\u6700\u5c0f\u6848\u4f8b\uff0c\u5e76\u8bb0\u5f55\u54ea\u4e9b\u8f93\u51fa\u5141\u8bb8\u6570\u503c\u8bef\u5dee\u3001\u54ea\u4e9b\u5fc5\u987b\u5b8c\u5168\u4e00\u81f4\u3002<\/p>\n

\u9002\u7528\u60c5\u51b5<\/h2>\n

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\u5efa\u8bae\u6309\u8fd9\u4e2a\u987a\u5e8f\u68c0\u67e5<\/h2>\n
    \n
  1. \u4e3a\u539f\u59cb\u6570\u636e\u3001\u5206\u7ec4\u8868\u3001\u57fa\u56e0\u96c6\u548c\u5916\u90e8\u6570\u636e\u5e93\u8bb0\u5f55\u6765\u6e90\u3001\u7248\u672c\u3001\u65e5\u671f\u53ca\u6587\u4ef6\u6821\u9a8c\u503c\uff0c\u4fdd\u5b58\u7eb3\u5165\u6392\u9664\u6e05\u5355\u3002<\/li>\n
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  3. \u4fdd\u5b58 R\/Python \u4f9d\u8d56\u3001\u64cd\u4f5c\u7cfb\u7edf\u3001\u7cfb\u7edf\u5e93\u3001\u5bb9\u5668\u6807\u8bc6\u3001CPU\/\u5185\u5b58\u548c\u5e76\u884c\u8bbe\u7f6e\uff0c\u968f\u673a\u8fc7\u7a0b\u8bb0\u5f55\u79cd\u5b50\u3002<\/li>\n
  4. \u4fdd\u7559\u4e2d\u95f4\u5173\u952e\u8f93\u51fa\u3001\u65e5\u5fd7\u548c\u5931\u8d25\u4fe1\u606f\uff0c\u5199\u660e\u6bcf\u4e2a\u6700\u7ec8\u56fe\u8868\u7531\u54ea\u4e00\u6b65\u751f\u6210\u3002<\/li>\n
  5. \u5728\u65b0\u4f1a\u8bdd\u6216\u72ec\u7acb\u73af\u5883\u4e2d\u6309\u8bf4\u660e\u91cd\u8dd1\u5c0f\u6848\u4f8b\uff0c\u6838\u5bf9\u8f93\u5165\u54c8\u5e0c\u3001\u6837\u672c\u987a\u5e8f\u3001\u5173\u952e\u6570\u503c\u548c\u8f93\u51fa\u6587\u4ef6\uff0c\u518d\u8bb0\u5f55\u5b9e\u9645\u9a8c\u8bc1\u8303\u56f4\u3002<\/li>\n<\/ol>\n

    \u4e00\u4e2a\u53ef\u590d\u7528\u7684\u9879\u76ee\u7ed3\u6784<\/h2>\n

    \u7ed3\u6784\u53ef\u4ee5\u8c03\u6574\uff0c\u5173\u952e\u662f\u80fd\u4ece\u6700\u7ec8\u56fe\u8868\u8ffd\u6eaf\u5230\u8f93\u5165\u548c\u4ee3\u7801\u3002\u53d7\u9650\u5236\u6570\u636e\u5e94\u4fdd\u7559\u5408\u89c4\u83b7\u53d6\u8bf4\u660e\u4e0e\u8bbf\u95ee\u63a7\u5236\uff0c\u4e0d\u56e0\u4e3a\u8ffd\u6c42\u590d\u73b0\u800c\u653e\u5230\u516c\u5f00\u4e0b\u8f7d\u76ee\u5f55\u3002<\/p>\n

    project\/\n  README.txt            # \u8fd0\u884c\u987a\u5e8f\u3001\u8f93\u5165\u83b7\u53d6\u65b9\u6cd5\u3001\u9a8c\u8bc1\u8303\u56f4\n  config\/               # \u53c2\u6570\u4e0e\u57fa\u56e0\u96c6\u7248\u672c\u5361\n  metadata\/             # \u5206\u7ec4\u3001\u6837\u672c\u5173\u7cfb\u3001\u7eb3\u5165\u6392\u9664\u8bb0\u5f55\n  scripts\/              # \u6709\u987a\u5e8f\u7684\u5206\u6790\u4ee3\u7801\n  environment\/          # sessionInfo\u3001\u9501\u6587\u4ef6\u3001\u7cfb\u7edf\u4f9d\u8d56\n  logs\/                 # \u6bcf\u6b65\u8fd0\u884c\u4e0e\u9519\u8bef\u65e5\u5fd7\n  results\/              # \u56fe\u8868\u3001\u5173\u952e\u6570\u503c\u548c\u9a8c\u6536\u7ed3\u679c\n  checksums.txt         # \u8f93\u5165\u6587\u4ef6\u6821\u9a8c\u503c<\/code><\/pre>\n
    \u6700\u5c11\u8981\u8bb0\u5f55\u7684\u53c2\u6570<\/h2>\n
    \n\n\n\n\n\n\n\n\n\n
    \u9636\u6bb5<\/th>\n\u5173\u952e\u5b57\u6bb5<\/th>\n\u5e38\u6f0f\u9879<\/th>\n<\/tr>\n<\/thead>\n
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    \u8bc4\u5206<\/td>\n\u65b9\u6cd5\u3001\u8f93\u5165\u5c42\u3001\u547d\u4e2d\u7387\u3001\u968f\u673a\u53c2\u6570<\/td>\n\u53ea\u4fdd\u7559\u6700\u540e\u7684\u5206\u6570<\/td>\n<\/tr>\n
    \u5efa\u6a21<\/td>\n\u7ed3\u5c40\u3001\u62c6\u5206\u3001\u7b5b\u9009\u3001\u8c03\u53c2\u3001\u9884\u5904\u7406<\/td>\n\u5916\u90e8\u9a8c\u8bc1\u88ab\u91cd\u65b0\u8c03\u53c2<\/td>\n<\/tr>\n
    \u8fd0\u884c\u73af\u5883<\/td>\n\u5305\u7248\u672c\u3001\u7cfb\u7edf\u5e93\u3001\u5e76\u884c\u4e0e\u6570\u636e\u5e93<\/td>\n\u53ea\u7559 R \u7248\u672c\u6ca1\u6709\u5916\u90e8\u8d44\u6e90<\/td>\n<\/tr>\n
    \u590d\u73b0\u9a8c\u6536<\/td>\n\u8fd0\u884c\u5165\u53e3\u3001\u6570\u503c\u5bb9\u5dee\u3001\u4ea7\u7269\u6e05\u5355<\/td>\n\u53ea\u8bf4\u201c\u80fd\u8fd0\u884c\u201d\u800c\u4e0d\u6838\u5bf9\u7ed3\u679c<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
    \u4f9d\u636e\uff1arenv\uff1a\u9879\u76ee\u73af\u5883\u3001\u9501\u6587\u4ef6\u548c\u6062\u590d<\/a> \u00b7 Seurat v5\uff1aassay\u3001layer \u4e0e\u6570\u636e\u8bbf\u95ee<\/a> \u00b7 TRIPOD \u5b98\u65b9\u8d44\u6e90\uff1a\u9884\u6d4b\u6a21\u578b\u5f00\u53d1\u4e0e\u9a8c\u8bc1\u6e05\u5355<\/a><\/p>\n
    \u4fdd\u5b58\u8f6f\u4ef6\u4fe1\u606f\u7684\u6700\u5c0f\u793a\u4f8b<\/h2>\n
    \u8be5\u793a\u4f8b\u53ea\u4fdd\u5b58\u5f53\u524d R \u4f1a\u8bdd\u4fe1\u606f\uff1b\u4e0d\u81ea\u52a8\u5b89\u88c5\u5305\u3001\u4e0d\u521b\u5efa\u5b8c\u6574\u9501\u6587\u4ef6\uff0c\u4e5f\u4e0d\u5305\u542b\u4f60\u7684\u7814\u7a76\u6570\u636e\u3002renv \u7684\u6062\u590d\u4ecd\u53d7\u7cfb\u7edf\u4f9d\u8d56\u548c\u5305\u6e90\u53ef\u83b7\u5f97\u6027\u5f71\u54cd\uff0c\u5bb9\u5668\u6807\u7b7e\u4e5f\u53ef\u80fd\u53d8\u5316\uff0c\u5173\u952e\u4ea4\u4ed8\u5e94\u4fdd\u5b58\u4e0d\u53ef\u53d8\u6807\u8bc6\u6216\u6821\u9a8c\u8bb0\u5f55\u3002<\/p>\n
    # \u4fdd\u5b58\u524d\u5148\u786e\u8ba4\u5f53\u524d\u76ee\u5f55\u662f\u672c\u9879\u76ee\uff1b\u6587\u4ef6\u540d\u4f7f\u7528\u65b0\u7684\u65f6\u95f4\u6233\nstamp <- format(Sys.time(), '%Y%m%d-%H%M%S')\nout <- paste0('session-info-', stamp, '.txt')\nstopifnot(!file.exists(out))\nwriteLines(capture.output(sessionInfo()), out)\n# \u5df2\u914d\u7f6e renv \u7684\u9879\u76ee\u53ef\u5728\u786e\u8ba4\u4f9d\u8d56\u540e\u53e6\u505a renv::snapshot()\n# \u5728\u9694\u79bb\u7684\u65b0\u9879\u76ee\u73af\u5883\u4e2d\u68c0\u9a8c renv::restore()\uff0c\u5e76\u8bb0\u5f55\u6210\u529f\u8303\u56f4<\/code><\/pre>\n
    \u4f9d\u636e\uff1arenv\uff1a\u9879\u76ee\u73af\u5883\u3001\u9501\u6587\u4ef6\u548c\u6062\u590d<\/a><\/p>\n
    \u4ec0\u4e48\u624d\u7b97\u5b8c\u6210\u4e86\u590d\u73b0\u68c0\u67e5<\/h2>\n
    \u6587\u4ef6\u80fd\u6253\u5f00\u53ea\u662f\u7b2c\u4e00\u6b65\u3002\u5e94\u68c0\u67e5\u6837\u672c\u6570\u91cf\u4e0e\u987a\u5e8f\u3001\u57fa\u56e0\u547d\u4e2d\u6570\u3001\u6bd4\u8f83\u65b9\u5411\u3001\u4e3b\u8981\u7edf\u8ba1\u91cf\u548c\u56fe\u8868\u5bf9\u5e94\u5173\u7cfb\u3002\u968f\u673a\u6216\u5e76\u884c\u8ba1\u7b97\u53ef\u80fd\u51fa\u73b0\u5408\u7406\u6570\u503c\u5dee\u5f02\uff0c\u5e94\u9884\u5148\u8bf4\u660e\u6bd4\u8f83\u65b9\u5f0f\u4e0e\u5bb9\u5dee\uff0c\u800c\u4e0d\u662f\u4fdd\u8bc1\u6240\u6709\u73af\u5883\u9010\u5b57\u8282\u76f8\u540c\u3002<\/p>\n
    \u672c\u4e13\u9898\u7684\u5c0f\u578b\u793a\u4f8b\u6d4b\u8bd5\u4e0d\u7b49\u4e8e\u6574\u7bc7\u8bba\u6587\u590d\u73b0\u3002\u771f\u5b9e\u9879\u76ee\u7684\u9a8c\u6536\u8bb0\u5f55\u5e94\u6ce8\u660e\u6570\u636e\u8303\u56f4\u3001\u547d\u4ee4\u3001\u73af\u5883\u3001\u65f6\u95f4\u4e0e\u8f93\u51fa\uff0c\u5e76\u660e\u786e\u672a\u8986\u76d6\u7684\u6b65\u9aa4\u3002<\/p>\n
    \u53ef\u76f4\u63a5\u4f7f\u7528\u7684\u8bb0\u5f55\u6a21\u677f<\/h2>\n