{"id":55112,"date":"2024-06-16T16:14:23","date_gmt":"2024-06-16T08:14:23","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=55112"},"modified":"2026-09-11T23:07:49","modified_gmt":"2026-09-11T15:07:49","slug":"%e3%80%90%e5%b0%8f%e4%ba%91%e8%a7%a3%e8%af%bb%e7%94%9f%e4%bf%a1%e5%9b%be%e3%80%91%e8%bf%99%e6%ac%a1%e8%ae%b2%e8%ae%b2%ef%bc%8c%e5%9f%ba%e5%9b%a0%e9%9b%86%e5%af%8c%e9%9b%86%e5%88%86%e6%9e%90gsea","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/%e3%80%90%e5%b0%8f%e4%ba%91%e8%a7%a3%e8%af%bb%e7%94%9f%e4%bf%a1%e5%9b%be%e3%80%91%e8%bf%99%e6%ac%a1%e8%ae%b2%e8%ae%b2%ef%bc%8c%e5%9f%ba%e5%9b%a0%e9%9b%86%e5%af%8c%e9%9b%86%e5%88%86%e6%9e%90gsea\/","title":{"rendered":"\u3010\u5c0f\u4e91\u89e3\u8bfb\u751f\u4fe1\u56fe\u3011\u8fd9\u6b21\u8bb2\u8bb2\uff0c\u57fa\u56e0\u96c6\u5bcc\u96c6\u5206\u6790GSEA\u56fe\u600e\u4e48\u770b?"},"content":{"rendered":"<aside class=\"ysx-source-note\"><span>\u6765\u6e90<\/span><\/p>\n<p>\u516c\u4f17\u53f7\u300c\u4e91\u751f\u4fe1\u5b66\u751f\u7269\u4fe1\u606f\u5b66\u300d\u5386\u53f2\u5185\u5bb9\uff1b\u672c\u6587\u4fdd\u7559\u539f\u6709\u6280\u672f\u4fe1\u606f\u5e76\u4f18\u5316\u7f51\u9875\u6392\u7248\u3002 <a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&amp;mid=2650748689&amp;idx=2&amp;sn=42b24ca11b3b2df47310d7e1910bd8d1\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">\u67e5\u770b\u539f\u6587<\/a><\/p>\n<\/aside>\n<p>\u5e08\u5144\uff0c\u5c31 \u4e0a\u6b21\u90a3\u7bc7\u7eaf\u751f\u4fe1\u5206\u6790\u7684\u6587\u7ae0\uff0c\u8fd9\u90fd\u6295\u51fa\u53bb\u4e00\u4e2a\u6708\u4e86\uff0c\u6211\u90fd\u6709\u4e9b\u4e0d\u6562\u767b\u90ae<\/p>\n<p>\u505a\u4e86\u751f\u4fe1\u5206\u6790\uff0c\u62ff\u5230\u4e00\u5806\u6570\u636e\uff0c\u770b\u4e0d\u61c2\u56fe\u600e\u4e48\u529e\uff1f<\/p>\n<p>\u706b\u5c71\u56fe\u3001\u70ed\u56fe\u3001\u6563\u70b9\u56fe\u3001\u7bb1\u5f0f\u56fe\u3001\u7011\u5e03\u56fe\u00b7\u00b7\u00b7\u8fd9\u4e48\u591a\u7c7b\u578b\u7684\u56fe\u90fd\u548b\u770b\uff1f<\/p>\n<p>\u98ce\u9669\u6a21\u578b\u9884\u540e\u8bc4\u4f30\u56fe\u3001GO-KEGG\u5bcc\u96c6\u5206\u6790\u56fe\u3001GSEA\u5bcc\u96c6\u5206\u6790\u56fe\u00b7\u00b7\u00b7\u8fd9\u4e9b\u56fe\u53c8\u4ee3\u8868\u4e86\u4ec0\u4e48\u7ed3\u679c\uff1f<\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_qgN9BLUIkC3TX7kWNKKwzVEwvvEyZA.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p>\u5c0f\u4e91\u6700\u65b0\u680f\u76ee\u3010\u5c0f\u4e91\u89e3\u8bfb\u751f\u4fe1\u56fe\u3011\u4e0a\u7ebf\u4ee5\u540e\uff0c\u8bb2\u4e86\u706b\u5c71\u56fe\u3001\u70ed\u56fe\u3001\u76f8\u5173\u6027\u6563\u70b9\u56fe\uff0c\u9605\u8bfb\u53cd\u9988\u90fd\u8fd8\u4e0d\u9519<\/p>\n<p>\u611f\u8c22\u7c89\u4e1d\u670b\u53cb\u4eec\u7684\u5173\u6ce8\uff0c\u5c0f\u4e91\u7684\u65b0\u680f\u76ee\u53ef\u4ee5\u7ee7\u7eed\u66f4\u65b0<\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_afVtXrYicEtEcW1jJd4xVIoQGtJB0Q.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p>\u5e94\u7c89\u4e1d\u670b\u53cb\u7684\u8981\u6c42\uff0c\u8fd9\u6b21\u8bb2\u8bb2 GSEA\u56fe<\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_NhcHMKcic8KApEexu8OBGdCxFOIGFw.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p>\u8bdd\u4e0d\u591a\u8bf4\uff0c\u5148\u770b\u56fe\uff0c\u8fd9\u5e94\u8be5\u662f\u5927\u5bb6\u6700\u5e38\u89c1\u5230\u7684GSEA\u56fe<\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_m4esnByHDSzyTEfo1yddrmJ608I11w.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p>\uff08 doi: 10.3389\/fimmu.2022.871564 \uff09<\/p>\n<h2>1. \u4ec0\u4e48\u662f GSEA<\/h2>\n<p>Gene Set Enrichment Analysis (GAES\uff0c\u57fa\u56e0\u96c6\u5bcc\u96c6\u5206\u6790)\u7528\u6765\u8bc4\u4f30\u4e00\u4e2a\u9884\u5148\u5b9a\u4e49\u7684\u57fa\u56e0\u96c6\u7684\u57fa\u56e0\u5728\u4e0e\u8868\u578b\u76f8\u5173\u5ea6\u6392\u5e8f\u7684\u57fa\u56e0\u8868\u4e2d\u7684\u5206\u5e03\u8d8b\u52bf\uff0c\u4ece\u800c\u5224\u65ad\u5176\u5bf9\u8868\u578b\u7684\u8d21\u732e\u3002<\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/9_LjuE7pKtPDfrk7UxY5BMMjicpIFUcg.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<h2>2. \u5df2\u6709 GO\/KEGG\uff0c\u4e3a\u4ec0\u4e48\u8fd8\u8981\u505a GSEA<\/h2>\n<p>GO\/KEGG\u5bcc\u96c6\u5206\u6790\u662f\u5148\u7b5b\u9009\u5dee\u5f02\u57fa\u56e0\uff0c\u518d\u5224\u65ad\u5dee\u5f02\u57fa\u56e0\u5728\u54ea\u4e9b\u6ce8\u91ca\u7684\u901a\u8def\u5b58\u5728\u5bcc\u96c6\uff1b\u8fd9\u6d89\u53ca\u5230\u9608\u503c\u7684\u8bbe\u5b9a\uff0c\u5b58\u5728\u4e00\u5b9a\u4e3b\u89c2\u6027\u5e76\u4e14\u53ea\u80fd\u7528\u4e8e\u8868\u8fbe\u53d8\u5316\u8f83\u5927\u7684\u57fa\u56e0\uff0c\u5373\u6211\u4eec\u5b9a\u4e49\u7684\u663e\u8457\u5dee\u5f02\u57fa\u56e0\u3002<\/p>\n<p>GSEA\u5219\u4e0d\u5c40\u9650\u4e8e\u5dee\u5f02\u57fa\u56e0\uff0c\u4e0d\u9700\u8981\u6307\u5b9a\u9608\u503c\uff08p\u503c\u6216FDR\uff09\u6765\u7b5b\u9009\u5dee\u5f02\u57fa\u56e0\uff0c\u6211\u4eec\u53ef\u4ee5\u5728\u6ca1\u6709\u9608\u503c\u9650\u5236\u7684\u60c5\u51b5\u4e0b\u5206\u6790\u6211\u4eec\u611f\u5174\u8da3\u7684\u57fa\u56e0\u96c6\uff0c\u800c\u8fd9\u4e2a\u57fa\u56e0\u96c6\u4e0d\u4e00\u5b9a\u662f\u663e\u8457\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u3002GSEA\u5206\u6790\u53ef\u4ee5\u5c06\u90a3\u4e9bGO\/KEGG\u5bcc\u96c6\u5206\u4fe1\u606f\u4e2d\u5bb9\u6613\u9057\u6f0f\u6389\u7684\u5dee\u5f02\u8868\u8fbe\u4e0d\u663e\u8457\u5374\u6709\u7740\u91cd\u8981\u751f\u7269\u5b66\u610f\u4e49\u7684\u57fa\u56e0\u5305\u542b\u5728\u5185\u3002<\/p>\n<h2>3. GSEA \u7684\u539f\u7406\u662f\u4ec0\u4e48<\/h2>\n<p>\u7ed9\u5b9a\u4e00\u4e2a\u6392\u5e8f\u7684\u57fa\u56e0\u8868L\uff08\u57fa\u56e0\u8868\u8fbe\u77e9\u9635\uff09\u548c\u4e00\u4e2a\u9884\u5148\u5b9a\u4e49\u7684\u57fa\u56e0\u96c6S (\u53ef\u4ee5\u662fGO\u6ce8\u91ca\u3001MsigDB\u7684\u6ce8\u91ca\u6216\u5176\u5b83\u7b26\u5408\u683c\u5f0f\u7684\u57fa\u56e0\u96c6\u5b9a\u4e49)\uff0c GSEA\u7684\u76ee\u7684\u662f\u5224\u65adS\u91cc\u9762\u7684\u6210\u5458s\u5728L\u91cc\u9762\u662f\u968f\u673a\u5206\u5e03\u8fd8\u662f\u4e3b\u8981\u805a\u96c6\u5728L\u7684\u9876\u90e8\u6216\u5e95\u90e8\u3002 \u8fd9\u4e9b\u57fa\u56e0\u6392\u5e8f\u7684\u4f9d\u636e\u662f\u5176\u5728\u4e0d\u540c\u8868\u578b\u72b6\u6001\u4e0b\u7684\u8868\u8fbe\u5dee\u5f02\uff0c\u82e5\u7814\u7a76\u7684\u57fa\u56e0\u96c6S\u7684\u6210\u5458\u663e\u8457\u805a\u96c6\u5728L\u7684\u9876\u90e8\u6216\u5e95\u90e8\uff0c\u5219\u8bf4\u660e\u6b64\u57fa\u56e0\u96c6\u6210\u5458\u5bf9\u8868\u578b\u7684\u5dee\u5f02\u6709\u8d21\u732e\uff0c\u4e5f\u662f\u6211\u4eec\u5173\u6ce8\u7684\u57fa\u56e0\u96c6\u3002\u4e0a\u4e2a\u56fe\u5c55\u793a\u4e00\u6ce2<\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/10_VFQTuAdCV3nicdDHRjYl3HX2McNeaA.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p>\uff08 doi: 10.1073\/pnas.0506580102 \uff09<\/p>\n<h2>4. GSEA \u56fe\u600e\u4e48\u770b<\/h2>\n<p>1)\u5bcc\u96c6\u5f97\u5206 (ES, enrichment score)\uff1a \u53cd\u5e94\u57fa\u56e0\u96c6\u6210\u5458s\u5728\u6392\u5e8f\u5217\u8868L\u7684\u4e24\u7aef\u5bcc\u96c6\u7684\u7a0b\u5ea6\u3002\u8ba1\u7b97\u65b9\u5f0f\u662f\uff0c\u4ece\u57fa\u56e0\u96c6L\u7684\u7b2c\u4e00\u4e2a\u57fa\u56e0\u5f00\u59cb\uff0c\u8ba1\u7b97\u4e00\u4e2a\u7d2f\u8ba1\u7edf\u8ba1\u503c\u3002\u5f53\u9047\u5230\u4e00\u4e2a\u843d\u5728s\u91cc\u9762\u7684\u57fa\u56e0\uff0c\u5219\u589e\u52a0\u7edf\u8ba1\u503c\u3002\u9047\u5230\u4e00\u4e2a\u4e0d\u5728s\u91cc\u9762\u7684\u57fa\u56e0\uff0c\u5219\u964d\u4f4e\u7edf\u8ba1\u503c \u3002 \u5bcc\u96c6\u5f97\u5206ES\u6700\u540e\u5b9a\u4e49\u4e3a\u6700\u5927\u7684\u5cf0\u503c\u3002\u6b63\u503cES\u8868\u793a\u57fa\u56e0\u96c6\u5728\u5217\u8868\u7684\u9876\u90e8\u5bcc\u96c6\uff0c\u8868\u793a\u5bcc\u96c6\u901a\u8def\u4e3a\u4e0a\u8c03\u901a\u8def\uff1b\u8d1f\u503cES\u8868\u793a\u57fa\u56e0\u96c6\u5728\u5217\u8868\u7684\u5e95\u90e8\u5bcc\u96c6\uff0c\u8868\u793a\u5bcc\u96c6\u901a\u8def\u4e3a\u4e0b\u8c03\u901a\u8def\u3002<\/p>\n<p>2)\u7ad6\u7ebf\uff1a \u56fe\u4e2d\u95f4\u90e8\u5206\u6bcf\u4e00\u6761\u7ebf\u4ee3\u8868\u57fa\u56e0\u96c6S\u4e2d\u7684\u4e00\u4e2a\u57fa\u56e0\uff0c\u53ca\u5176\u5728\u57fa\u56e0\u5217\u8868L\u4e2d\u7684\u6392\u5e8f\u4f4d\u7f6e\u3002<\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/11_PFHdLc1icibvETbbqvNcRdhg5omZkA.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p>3)Leading-edge subset (\u9886\u5934\u4e9a\u96c6): \u5bf9\u5bcc\u96c6\u5f97\u5206\u8d21\u732e\u6700\u5927\u7684\u57fa\u56e0\u6210\u5458\uff0c\u4e5f\u662f\u540e\u7eed\u7814\u7a76\u4e2d\u91cd\u70b9\u5173\u6ce8\u7684\u901a\u8def\u5173\u952e\u57fa\u56e0\u3002\u82e5\u5bcc\u96c6\u5f97\u5206\u4e3a\u6b63\u503c\uff0c\u5219\u662f\u5cf0\u5de6\u4fa7\u7684\u57fa\u56e0\uff1b\u82e5\u5bcc\u96c6\u5f97\u5206\u4e3a\u8d1f\u503c\uff0c\u5219\u662f\u5cf0\u53f3\u4fa7\u7684\u57fa\u56e0\u3002<\/p>\n<p>4)\u663e\u8457\u6027\u5224\u65ad\uff08 p\u503c\uff09<\/p>\n<p>P\u503c\u662f\u7528\u6765\u8fdb\u884c\u663e\u8457\u6027\u68c0\u9a8c\u7684\uff0c\u7528\u6765\u8bc4\u4f30\u5bcc\u96c6\u5f97\u5206(ES)\u7684\u663e\u8457\u6027\uff0cP\u503c&lt;0.05\uff0c\u4ee3\u8868S\u57fa\u56e0\u96c6\u5728\u5217\u8868L\u4e2d\u663e\u8457\u5bcc\u96c6\uff0c\u7b80\u5355\u70b9\u6765\u8bf4\uff0c\u5c31\u662f\u5173\u6ce8\u67d0\u4e2a\u901a\u8def\u5728\u75be\u75c5\u4e2d\u663e\u8457\u5bcc\u96c6\u3002<\/p>\n<p>\u518d\u6765\u5f20\u4e0d\u4e00\u6837\u7684<\/p>\n<p>\uff08 ps \uff1a\u4e0d\u4e00\u6837\u7684\u53ea\u662f\u5f62\u5f0f\uff0c\u89e3\u56fe\u7684\u65b9\u6cd5\u8fd8\u662f\u4e0d\u53d8\u6ef4\uff0c\u4e07\u53d8\u4e0d\u79bb\u5176\u5b97 \uff09<\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/12_J5sVReTlfKZROH0NKh3CBTnxIuaeCw.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p>\uff08\u628a\u591a\u4e2a\u901a\u8def\u7684\u57fa\u56e0\u96c6\u653e\u5728\u4e00\u4e2a\u56fe\u4e2d\u5c55\u793a\uff0c doi: 10.1155\/2022\/4254195 \uff09<\/p>\n<p>G SEA \u56fe\u7684\u89e3\u8bfb\u6709\u6ca1\u6709\u8ba9\u751f\u4fe1\u5c0f\u767d\u4eec\u8c41\u7136\u5f00\u6717\u5440\uff1f\u8fd8\u6709\u95ee\u9898\u7684\u8bdd\u6b22\u8fce\u7ed9\u5c0f\u4e91\u7559\u8a00\u6216\u79c1\u4fe1\u54e6\uff0c\u4e3a\u4e86\u8ba9\u8fd9\u4e2a\u7cfb\u5217\u80fd\u4e00\u76f4\u66f4\u65b0\uff0c\u670b\u53cb\u4eec\u5feb\u6765\u591a\u591a\u7559\u8a00\u652f\u6301\u5427<\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/13_0UicKqQUrnEGEeIJUUXKODzMI5Mz4g.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u5c0f\u4e91\u89e3\u8bfb\u751f\u4fe1\u56fe\u3011\u8fd9\u6b21\u8bb2\u8bb2\uff0c\u57fa\u56e0\u96c6\u5bcc\u96c6\u5206\u6790GSEA\u56fe\u600e\u4e48\u770b? eryun \u751f\u4fe1\u679c 2023-12-27 19:00:31 \u8f6c\u81ea\u516c\u4f17\u53f7\uff1a\u4e91\u751f\u4fe1\u5b66\u751f\u7269\u4fe1\u606f\u5b66http:\/\/mp.weixin.qq.com\/s?__biz=MzAwNjE0MDY3MQ==&#038;mid=2650\u2026<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[1,73],"tags":[],"class_list":["post-55112","post","type-post","status-publish","format-standard","hentry","category-part","category-enrichment"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/55112","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=55112"}],"version-history":[{"count":2,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/55112\/revisions"}],"predecessor-version":[{"id":65430,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/55112\/revisions\/65430"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=55112"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=55112"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=55112"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}