{"id":57741,"date":"2024-06-16T20:30:21","date_gmt":"2024-06-16T12:30:21","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=57741"},"modified":"2026-09-11T23:07:49","modified_gmt":"2026-09-11T15:07:49","slug":"%e3%80%90%e4%b8%80%e6%96%87%e4%b8%80%e5%88%86%e9%92%9f%e3%80%91%e5%b8%a6%e4%bd%a0%e8%af%bb%e6%87%82wgcna%e7%bb%93%e6%9e%9c%e5%9b%be","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/%e3%80%90%e4%b8%80%e6%96%87%e4%b8%80%e5%88%86%e9%92%9f%e3%80%91%e5%b8%a6%e4%bd%a0%e8%af%bb%e6%87%82wgcna%e7%bb%93%e6%9e%9c%e5%9b%be\/","title":{"rendered":"\u3010\u4e00\u6587\u4e00\u5206\u949f\u3011\u5e26\u4f60\u8bfb\u61c2WGCNA\u7ed3\u679c\u56fe"},"content":{"rendered":"<aside class=\"ysx-source-note\"><span>\u6765\u6e90<\/span><\/p>\n<p>\u516c\u4f17\u53f7\u300c\u751f\u4fe1\u56fe\u300d\u5386\u53f2\u5185\u5bb9\uff1b\u672c\u6587\u4fdd\u7559\u539f\u6709\u6280\u672f\u4fe1\u606f\u5e76\u4f18\u5316\u7f51\u9875\u6392\u7248\u3002 <a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzkxODUxNDgzNA==&amp;mid=2247484147&amp;idx=1&amp;sn=2da79fb1b229161ed80fedc93ba94537\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">\u67e5\u770b\u539f\u6587<\/a><\/p>\n<\/aside>\n<p>Hello~\u53c8\u5230\u5c0f\u56fe\u3010\u4e00\u6587\u4e00\u5206\u949f\u3011\u8bfe\u5802\u65f6\u95f4<\/p>\n<p>\u524d\u9762\u7684\u6587\u7ae0\u5c0f\u56fe\u4ecb\u7ecd\u4e86WGCNA\uff0c\u5927\u5bb6\u53bb\u641c\u7d22WGCNA\u80af\u5b9a\u4e5f\u4f1a\u627e\u5230\u5f88\u591a\u6559\u7a0b\u3002\u5176\u5b9e\u6211\u4eec\u6700\u91cd\u8981\u7684\u4e00\u6b65\u662f\u7406\u89e3\u7b97\u6cd5\u7684\u539f\u7406\uff0c\u8dd1\u5b8c\u8fd9\u4e2a\u4ee3\u7801\uff0c\u80fd\u591f\u5bf9\u7ed3\u679c\u8fdb\u884c\u4e00\u4e2a\u6b63\u786e\u7684\u89e3\u8bfb\u90a3\u4e48\u5982\u4f55\u89e3\u8bfbWGCNA\u7684\u7ed3\u679c\uff1f\u8fd9\u4e00\u6587\u5c0f\u56fe\u4f1a\u8fdb\u884c\u8be6\u7ec6\u4ecb\u7ecd\u3002<\/p>\n<p>\u4eca\u5929\u4e0d\u8bb2\u4ee3\u7801\uff0c\u53ea\u8bb2\u8bb2\u8fd9\u4e9b\u56fe\u600e\u4e48\u770b\uff0c\u80fd\u8868\u8fbe\u4ec0\u4e48\u5185\u5bb9\u3002\u60f3\u770b\u4ee3\u7801\u53ef\u4ee5\u79fb\u6b65 \u4f60\u7684\u9ad8\u5206\u6587\u7ae0\u8fd8\u5dee\u4e00\u5957WGCNA\u5206 \u540e\u518d\u8fd4\u56de\u770b\u6b64\u6587<\/p>\n<p>WGCNA\u5206\u6790\u6d41\u7a0b\u56fe\u4e3b\u8981\u5305\u62ec\uff1a\u6784\u5efa\u5171\u8868\u8fbe\u7f51\u7edc\u2192\u6a21\u5757\u8bc6\u522b\u2192\u6a21\u5757\u4e0e\u6027\u72b6\u5173\u8054\u2192\u6a21\u5757\u76f8\u5173\u6027\u2192\u5173\u952e\u6a21\u5757\u4e2d\u8bc6\u522b\u6838\u5fc3\u57fa\u56e0<\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/1_Yr614dUYUx9A6xhXP9hOqYLn1icWpg.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<h2>1. \u6837\u672c\u805a\u7c7b\u4e0e\u5f02\u5e38\u6837\u672c<\/h2>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/2_ee0iaJunMicmKNtxPiaGhbW9dXcw9w.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p><strong>\u8f93\u51fa\u6587\u4ef6\u89e3\u8bfb<\/strong><\/p>\n<p>\u7ed3\u679c\u663e\u793a\uff1a\u53ef\u4ee5\u770b\u5230\uff0c\u6700\u5de6\u8fb9\u660e\u663e\u6709\u51e0\u4e2a\u6837\u54c1\u548c\u522b\u4eba\u4e0d\u5728\u4e00\u6761\u679d\u53f6\u4e0a\uff0c\u56e0\u6b64\uff0c\u6211\u4eec\u9700\u8981\u5c06\u90a3\u51e0\u4e2a\u660e\u663e\u504f\u79bb\u7684\u5206\u652f\u7ed9\u526a\u53bb\u3002<\/p>\n<h2>2. \u9009\u62e9\u8f6f\u9608\u503c<\/h2>\n<p><strong>\u4e8c\u3001\u5728\u6784\u5efa\u5171\u8868\u8fbe\u7f51\u7edc\u4e4b\u524d\uff0c\u8fd8\u6709\u4e00\u6b65\u6bd4\u8f83\u5173\u952e\u7684\u6b65\u9aa4\u2014\u2014\u5bfb\u627e\u6700\u4f18\u8f6f\u9608\u503c\uff08soft thresholding\u6216power\uff09\uff0c\u4f7f\u6784\u5efa\u7684\u7f51\u7edc\u66f4\u7b26\u5408\u65e0\u6807\u5ea6\u62d3\u6251\u7ed3\u6784\u3002\u8fd9\u65f6\uff0c\u6211\u4eec\u4f1a\u770b\u5230\u5982\u4e0b\u56fe\uff1a<\/strong><\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/3_r8eb4zSBTleIU75vrNcD0GFLYEl1hQ.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p><strong>\u8f93\u51fa\u6587\u4ef6\u89e3\u8bfb<\/strong><\/p>\n<p>Scale independence<\/p>\n<p>Power\u4e00\u822c\u9009\u572816\u4ee5\u4e0b\u00b7R\u65b9\u8981\u9ad8\u4e8e0.8\u3002\u5982\u679c\u8c03\u8bd5\u540e\u8fd8\u4e0d\u80fd\u6ee1\u8db3\u53ef\u80fd\u662f\u8868\u8fbe\u77e9\u9635\u6ca1\u6709\u5904\u7406\u6070\u5f53\uff0c\u6216\u8005\u4e0d\u6ee1\u8db3\u5206\u6790\u6761\u4ef6\u6bd4\u5982\u6837\u672c\u91cc\u592a\u5c11\uff0c\u57fa\u56e0\u6570\u76ee\u8fc7\u591a\u7b49\u539f\u56e0(\u53d1\u8868\u6587\u7ae0\u4e00\u822c\u4e0d\u653e\u6b63\u6587)<\/p>\n<p>\u6839\u636e\u7ed3\u679c\uff0c\u7ed8\u5236power\u503c\u5bf9\u5e94\u7684\u6563\u70b9\u56fe\uff1b\u7ed3\u679c\u663e\u793a\uff0c1\u52302\uff0c3\u52304\uff0c\u6574\u4e2a\u53d8\u5316\u8d8b\u52bf\u90fd\u8fd8\u5f88\u660e\u663e\uff0c\u800c5\u5f80\u540e\uff0c\u968f\u7740power\u503c\u7684\u589e\u5927\uff0c\u5176\u53d8\u5316\u8d8b\u52bf\u5df2\u7ecf\u4e0d\u660e\u663e\u4e86\uff1b\u56e0\u6b64\uff0c\u9009\u53d67\u6216\u80058\u4e3a\u6700\u4f73\u7684power\u503c\u3002<\/p>\n<p>mean connectivity\uff08\u4e3a\u4e0d\u540c\u8f6f\u9608\u503c\u60c5\u51b5\u4e0b\u7684\u7f51\u7edc\u8fde\u901a\u5ea6\uff09<\/p>\n<p>\u5e73\u5747\u8fde\u63a5\u5ea6\u548cpower\u53d6\u503c\u95f4\u5173\u7cfb\u9009\u53d6power\u822c\u5728\u5e73\u5747\u8fde\u63a5\u5ea6\u53d8\u5316\u5448\u5e73\u7a33\u5904(\u53d1\u8868\u6587\u7ae0\u4e00\u822c\u4e0d\u653e\u6b63\u6587)<\/p>\n<h2>3. \u6784\u5efa\u7f51\u7edc\u5e76\u8bc6\u522b\u6a21\u5757<\/h2>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/4_RXwTIIPELxY1U9B2pyob1M1nl8iceA.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p><strong>\u8f93\u51fa\u6587\u4ef6\u89e3\u8bfb<\/strong><\/p>\n<p>Cluster Dendrogram<\/p>\n<p>\u805a\u7c7b\u6811\u72b6\u56fe\uff0c\u8fd9\u4e2a\u56fe\u53ef\u4ee5\u5206\u4e3a\u4e24\u90e8\u5206\u770b\uff1a\u4e0a\u534a\u90e8\u5206\u662f\u57fa\u56e0\u7684\u5c42\u6b21\u805a\u7c7b\u6811\u72b6\u56fe\uff0c\u4e0b\u534a\u90e8\u5206\u662f\u57fa\u56e0\u6a21\u5757\uff0c\u4e5f\u5c31\u662f\u7f51\u7edc\u6a21\u5757\u3002\u4e0a\u4e0b\u5bf9\u5e94\uff0c\u53ef\u4ee5\u770b\u5230\u8ddd\u79bb\u8f83\u8fd1\u7684\u57fa\u56e0\uff08\u805a\u7c7b\u5230\u540c\u4e00\u6761\u5206\u652f\uff09\u88ab\u5212\u5206\u5230\u4e86\u540c\u4e00\u6a21\u5757\u3002Merged\u662f\u5408\u5e76\u76f8\u4f3c\u6a21\u5757\u4e4b\u540e\u7684\u989c\u8272\u3002\u6bcf\u4e2a\u989c\u8272\u4ee3\u8868\u4e00\u4e2a\u6a21\u5757\uff0c\u7070\u8272\u4ee3\u8868\u91cc\u9762\u57fa\u56e0\u4e0d\u5c5e\u4e8e\u4efb\u4f55\u6a21\u5757\u3002<\/p>\n<h2>4. \u89e3\u8bfb\u6a21\u5757\u2014\u6027\u72b6\u5173\u7cfb<\/h2>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/5_fwXZPJtsTfJ3y8fZP8EW516L0dIbpg.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p><strong>\u8f93\u51fa\u6587\u4ef6\u89e3\u8bfb<\/strong><\/p>\n<p>Module-trait relationships<\/p>\n<p>\u4e0a\u56fe\u4e2d\uff0c\u6700\u5de6\u4fa7\u7684\u989c\u8272\u5757\u4ee3\u8868\u6a21\u5757\uff0c\u6700\u53f3\u4fa7\u7684\u989c\u8272\u6761\u4ee3\u8868\u76f8\u5173\u6027\u8303\u56f4\u3002\u4e2d\u95f4\u90e8\u5206\u7684\u70ed\u56fe\u4e2d\uff0c\u989c\u8272\u8d8a\u6df1\u76f8\u5173\u6027\u8d8a\u9ad8\uff0c\u7ea2\u8272\u8868\u793a\u6b63\u76f8\u5173\uff0c\u84dd\u8272\u8868\u793a\u8d1f\u76f8\u5173\uff1b\u6bcf\u4e2a\u5355\u5143\u683c\u4e2d\u7684\u6570\u5b57\u8868\u793a\u76f8\u5173\u6027\u548c\u663e\u8457\u6027\u3002\u5982\u4e0a\u56fe\uff0cplink\u6a21\u5757\u4e0e\u6027\u72b6\u8868\u73b0\u4e3a\u6b63\u76f8\u5173\u4e14\u76f8\u5173\u6027\u6700\u9ad8\u3002\u6b64\u65f6\uff0c\u6211\u4eec\u53ef\u4ee5\u9009\u62e9\u76f8\u5173\u6027\u6700\u9ad8\u7684plink\u6a21\u5757\u4f5c\u4e3a\u5173\u952e\u6a21\u5757\u3002\u4e00\u822c\uff0c\u6211\u4eec\u4f1a\u6309\u76f8\u5173\u6027\u7684\u7edd\u5bf9\u503c\u7b5b\u9009\u6700\u76f8\u5173\u6a21\u5757\uff0c\u5373\u8d1f\u76f8\u5173\u6a21\u5757\u4e5f\u5e94\u8be5\u8003\u8651\u5728\u5185\u3002\u9700\u8981\u6ce8\u610f\u7684\u662f\uff0cgrey\u6a21\u5757\u4e2d\u5305\u542b\u4e86\u6240\u6709\u672a\u53c2\u4e0e\u805a\u7c7b\u7684\u57fa\u56e0\uff0c\u56e0\u6b64\u662f\u65e0\u6548\u6a21\u5757\uff0c\u4e0d\u5e94\u7528\u4e8e\u540e\u7eed\u5206\u6790\u3002<\/p>\n<h2>5. \u67e5\u770b\u6a21\u5757\u95f4\u5173\u7cfb<\/h2>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/6_wmuRBPibIu8PIBiaCiaUPX4EeMgA7A.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p>\u4e4b\u540e\u9488\u5bf9\u5173\u952e\u6a21\u5757plink\u548c\u611f\u5174\u8da3\u7684\u6027\u72b6\u8fdb\u4e00\u6b65\u6316\u6398\uff0c\u770b\u770b\u57fa\u56e0\u4e0e\u6a21\u5757\u7684\u76f8\u5173\u6027\uff08Module Membership, MM\uff09\u548c\u57fa\u56e0\u4e0e\u6027\u72b6\u7684\u76f8\u5173\u6027\uff08Gene Significance, GS\uff09\u4e4b\u95f4\u662f\u5426\u6709\u67d0\u79cd\u5173\u8054\u3002<\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/7_D1riaLXwJeYe56RkpSFutBjS35icqA.png?ssl=1\" loading=\"lazy\" decoding=\"async\" alt=\"\"><\/figure>\n<p><strong>\u8f93\u51fa\u6587\u4ef6\u89e3\u8bfb<\/strong><\/p>\n<p>\u901a\u8fc7\u4ee5\u4e0a\u6563\u70b9\u56fe\uff0c\u53ef\u4ee5\u53d1\u73b0MM\u548cGS\u5448\u6b63\u76f8\u5173\uff0c\u8bf4\u660e\u8fd9\u4e9b\u4e0e\u6027\u72b6\u9ad8\u5ea6\u76f8\u5173\u7684\u57fa\u56e0\uff0c\u5728\u5173\u952e\u6a21\u5757\u4e2d\u4e5f\u626e\u6f14\u7740\u4e3e\u8db3\u8f7b\u91cd\u7684\u89d2\u8272\u3002<\/p>\n<h2>6. \u7b5b\u9009\u6838\u5fc3\u57fa\u56e0\u5e76\u7ed8\u5236\u7f51\u7edc<\/h2>\n<p><strong>\u516d\u3001\u6211\u4eec\u4e5f\u53ef\u4ee5\u57fa\u4e8e\u5173\u952e\u6a21\u5757\u4e2d\u57fa\u56e0\u4e4b\u95f4\u7684\u76f8\u4f3c\u6027\uff0c\u6784\u5efa\u5173\u952e\u6a21\u5757\u7f51\u7edc\uff0c\u4ee5Cytoscape\u8f6f\u4ef6\u53ef\u89c6\u5316\u5c55\u793a\u7f51\u7edc\u4e2d\u57fa\u56e0\u4e4b\u95f4\u7684\u4e92\u4f5c\u5173\u7cfb\uff1a<\/strong><\/p>\n<figure><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2024\/06\/8_EGeMINhtFgPIG13RV8AmSbP4tHUl3w.png?ssl=1\" loading=\"lazy\" decoding=\"async\" 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\u5c0f\u56fe \u751f\u4fe1\u679c 2023-08-10 19:02:16 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