{"id":65487,"date":"2026-09-17T10:23:15","date_gmt":"2026-09-17T02:23:15","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=65487"},"modified":"2026-09-17T10:23:15","modified_gmt":"2026-09-17T02:23:15","slug":"single-cell-qc-annotation-analysis-guide","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/single-cell-qc-annotation-analysis-guide\/","title":{"rendered":"\u5355\u7ec6\u80de\u5206\u6790\u600e\u4e48\u505a\uff1f\u5148\u7406\u6e05\u8d28\u63a7\u3001\u6ce8\u91ca\u4e0e\u7ec4\u95f4\u6bd4\u8f83"},"content":{"rendered":"<p>\u5355\u7ec6\u80de\u5206\u6790\u8dd1\u51fa\u4e86 UMAP\uff0c\u5374\u4e0d\u77e5\u9053\u7ec6\u80de\u5206\u7fa4\u662f\u5426\u53ef\u9760\u3001\u4e0b\u4e00\u6b65\u8be5\u505a\u4ec0\u4e48\uff1f\u5148\u56de\u5230\u4e09\u4e2a\u57fa\u7840\u95ee\u9898\uff1a<strong>\u7559\u4e0b\u6765\u7684\u7ec6\u80de\u8d28\u91cf\u5982\u4f55\uff0c\u7ec6\u80de\u8eab\u4efd\u662f\u5426\u53ef\u4fe1\uff0c\u7ec4\u95f4\u6bd4\u8f83\u80fd\u5426\u652f\u6301\u7814\u7a76\u7ed3\u8bba\u3002<\/strong><\/p>\n<h2>1. \u8d28\u63a7\u9608\u503c\u8981\u7ed3\u5408\u6837\u672c\uff0c\u4e0d\u80fd\u7167\u642c\u6559\u7a0b<\/h2>\n<p>\u5728 Seurat \u4e2d\uff0c\u5e38\u89c1\u6307\u6807\u5305\u62ec\u68c0\u6d4b\u5230\u7684\u57fa\u56e0\u6570\uff08<code>nFeature_RNA<\/code>\uff09\u3001UMI \u603b\u6570\uff08<code>nCount_RNA<\/code>\uff09\u548c\u7ebf\u7c92\u4f53\u8f6c\u5f55\u672c\u6bd4\u4f8b\uff08\u5e38\u8bb0\u4e3a <code>percent.mt<\/code>\uff09\u3002\u5efa\u8bae\u6309\u6837\u672c\u67e5\u770b\u6307\u6807\u5206\u5e03\uff0c\u518d\u51b3\u5b9a\u8fc7\u6ee4\u8303\u56f4\uff1b\u4e0d\u80fd\u53ea\u51ed\u4e00\u4e2a\u7ebf\u7c92\u4f53\u6bd4\u4f8b\u9608\u503c\u5224\u65ad\u6240\u6709\u7ec4\u7ec7\u7684\u7ec6\u80de\u8d28\u91cf\u3002\u57fa\u56e0\u6570\u5f02\u5e38\u504f\u9ad8\u8fd8\u5e94\u7ed3\u5408\u53cc\u7ec6\u80de\u68c0\u6d4b\u8fdb\u4e00\u6b65\u6392\u67e5\u3002\u5177\u4f53\u64cd\u4f5c\u53ef\u53c2\u8003 <a href=\"https:\/\/satijalab.org\/seurat\/articles\/pbmc3k_tutorial\" target=\"_blank\" rel=\"noopener noreferrer\">Seurat \u5b98\u65b9\u8d28\u63a7\u4e0e\u805a\u7c7b\u6559\u7a0b<\/a>\u3002<\/p>\n<h2>2. \u805a\u7c7b\u5b8c\u6210\u540e\uff0c\u8fd8\u9700\u8981\u6838\u5bf9\u7ec6\u80de\u8eab\u4efd<\/h2>\n<p>\u805a\u7c7b\u662f\u5728\u8868\u8fbe\u7279\u5f81\u4e0a\u5212\u5206\u7ec6\u80de\uff0c\u6ce8\u91ca\u5219\u9700\u8981\u751f\u7269\u5b66\u8bc1\u636e\u3002\u4e0d\u8981\u770b\u5230\u4e00\u4e2a\u6807\u5fd7\u57fa\u56e0\u8868\u8fbe\uff0c\u5c31\u76f4\u63a5\u786e\u5b9a\u7ec6\u80de\u7c7b\u578b\uff1b\u5e94\u7ed3\u5408\u591a\u4e2a\u6807\u5fd7\u57fa\u56e0\u3001\u8868\u8fbe\u6bd4\u4f8b\u548c\u7ec4\u7ec7\u6765\u6e90\u7efc\u5408\u5224\u65ad\u3002<\/p>\n<p>\u540c\u65f6\u68c0\u67e5\u5404\u7c07\u7684\u6837\u672c\u6784\u6210\uff1a\u5982\u679c\u67d0\u4e2a\u7c07\u51e0\u4e4e\u53ea\u6765\u81ea\u4e00\u4e2a\u6837\u672c\uff0c\u8981\u8fdb\u4e00\u6b65\u6392\u67e5\u6280\u672f\u6279\u6b21\u3001\u8d28\u91cf\u5dee\u5f02\u6216\u4e2a\u4f53\u7279\u5f02\u6027\u3002UMAP \u5206\u5f97\u6f02\u4eae\uff0c\u53ea\u80fd\u4f5c\u4e3a\u89c2\u5bdf\u7ebf\u7d22\u3002<\/p>\n<h2>3. \u505a\u75be\u75c5\u6216\u5904\u7406\u7ec4\u6bd4\u8f83\uff0c\u8981\u4fdd\u7559\u6837\u672c\u5c42\u9762\u7684\u4fe1\u606f<\/h2>\n<p>\u4f8b\u5982\uff0c\u60f3\u7814\u7a76\u201c\u5904\u7406\u540e\u5de8\u566c\u7ec6\u80de\u7684\u708e\u75c7\u72b6\u6001\u662f\u5426\u6539\u53d8\u201d\uff0c\u5e94\u5148\u786e\u8ba4\u6ce8\u91ca\uff0c\u518d\u5728\u540c\u7c7b\u7ec6\u80de\u4e2d\u6bd4\u8f83\u3002\u6765\u81ea\u540c\u4e00\u6837\u672c\u7684\u6570\u5343\u4e2a\u7ec6\u80de\uff0c\u4e0d\u80fd\u7b80\u5355\u5f53\u4f5c\u6570\u5343\u4e2a\u72ec\u7acb\u751f\u7269\u5b66\u91cd\u590d\u3002\u5177\u5907\u5408\u9002\u7684\u91cd\u590d\u8bbe\u8ba1\u65f6\uff0c\u53ef\u6309\u201c\u6837\u672c \u00d7 \u7ec6\u80de\u7c7b\u578b\u201d\u6c47\u603b\u8ba1\u6570\uff0c\u5f00\u5c55 pseudobulk \u5dee\u5f02\u5206\u6790\uff0c\u518d\u89e3\u91ca\u76f8\u5173\u901a\u8def\uff1b\u53c2\u89c1 <a href=\"https:\/\/satijalab.org\/seurat\/articles\/de_vignette\" target=\"_blank\" rel=\"noopener noreferrer\">Seurat \u6837\u672c\u5c42\u9762\u5dee\u5f02\u5206\u6790\u793a\u4f8b<\/a>\u3002<\/p>\n<p>\u4fdd\u5b58\u6837\u672c\u5206\u7ec4\u3001\u8fc7\u6ee4\u53c2\u6570\u3001\u6ce8\u91ca\u4f9d\u636e\u3001\u4ee3\u7801\u548c\u8f6f\u4ef6\u7248\u672c\uff0c\u624d\u80fd\u8ba9\u7ed3\u679c\u65b9\u4fbf\u590d\u67e5\uff0c\u4e5f\u4fbf\u4e8e\u540e\u7eed\u6df1\u5165\u5206\u6790\u3002<\/p>\n<h2>\u6839\u636e\u5f53\u524d\u5361\u70b9\uff0c\u9009\u62e9\u5408\u9002\u7684\u652f\u6301<\/h2>\n<p>\u5e0c\u671b\u7cfb\u7edf\u638c\u63e1\u6d41\u7a0b\uff0c\u53ef\u4ee5\u4e86\u89e3\u4e91\u751f\u4fe1\u7684<a href=\"https:\/\/www.biocloudservice.com\/course-preview\/\">\u751f\u7269\u4fe1\u606f\u5b66\u8bfe\u7a0b<\/a>\uff1b\u672c\u5730\u5185\u5b58\u6216\u8ba1\u7b97\u8d44\u6e90\u4e0d\u8db3\uff0c\u53ef\u4ee5\u54a8\u8be2<a href=\"https:\/\/www.biocloudservice.com\/server-calculator\/\">\u751f\u7269\u4fe1\u606f\u6570\u636e\u670d\u52a1\u5668\u79df\u8d41<\/a>\uff1b\u9700\u8981\u68b3\u7406\u5206\u6790\u8def\u7ebf\u3001\u7ec6\u80de\u6ce8\u91ca\u6216\u7ed3\u679c\u89e3\u8bfb\uff0c\u53ef\u4ee5\u4e86\u89e3<a href=\"https:\/\/www.biocloudservice.com\/wordpress\/services\/\">\u751f\u7269\u4fe1\u606f\u5b66\u5b9a\u5236\u5316\u5206\u6790<\/a>\u3002<\/p>\n<p><a href=\"https:\/\/work.weixin.qq.com\/kfid\/kfcb05492f29d4d6064\" target=\"_blank\" rel=\"noopener noreferrer\" data-ysx-event=\"contact_click\"><strong>\u8054\u7cfb\u4e91\u751f\u4fe1<\/strong><\/a>\uff0c\u8bf4\u660e\u7ec4\u7ec7\u7c7b\u578b\u3001\u6837\u672c\u5206\u7ec4\u3001\u7ec6\u80de\u89c4\u6a21\u548c\u5f53\u524d\u9047\u5230\u7684\u95ee\u9898\uff0c\u4e00\u8d77\u660e\u786e\u4e0b\u4e00\u6b65\u3002<\/p>\n<figure class=\"wp-block-image size-full\" style=\"margin:28px auto;max-width:520px\"><a href=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/09\/single-cell-analysis-poster-20260917.png?ssl=1\" target=\"_blank\" rel=\"noopener noreferrer\"><img data-recalc-dims=\"1\" class=\"no-photon\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/09\/single-cell-analysis-poster-20260917.png?resize=941%2C1672&#038;ssl=1\" alt=\"\u4e91\u751f\u4fe1\u5355\u7ec6\u80de\u5206\u6790\u652f\u6301\uff1a\u8d28\u63a7\u3001\u9884\u5904\u7406\u3001\u805a\u7c7b\u6ce8\u91ca\u4e0e\u7ed3\u679c\u89e3\u8bfb\uff0c\u626b\u7801\u54a8\u8be2\" width=\"941\" height=\"1672\" loading=\"lazy\" decoding=\"async\" style=\"display:block;width:100%;height:auto\"><\/a><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u4ece\u8d28\u63a7\u6307\u6807\u3001\u7ec6\u80de\u6ce8\u91ca\u5230\u6837\u672c\u5c42\u9762\u7684\u7ec4\u95f4\u6bd4\u8f83\uff0c\u7406\u6e05\u5355\u7ec6\u80de\u5206\u6790\u7684\u4e09\u4e2a\u5173\u952e\u73af\u8282\uff0c\u8ba9\u7ed3\u679c\u66f4\u4fbf\u4e8e\u89e3\u91ca\u548c\u590d\u67e5\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[1,71],"tags":[115,90,116,95],"class_list":["post-65487","post","type-post","status-publish","format-standard","hentry","category-part","category-single-cell","tag-seurat","tag-90","tag-116","tag-95"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/65487","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=65487"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/65487\/revisions"}],"predecessor-version":[{"id":65490,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/65487\/revisions\/65490"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=65487"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=65487"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=65487"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}