{"id":65505,"date":"2026-09-22T15:00:00","date_gmt":"2026-09-22T07:00:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=65505"},"modified":"2026-09-20T16:43:30","modified_gmt":"2026-09-20T08:43:30","slug":"spatial-transcriptomics-sample-analysis-project-checklist","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/spatial-transcriptomics-sample-analysis-project-checklist\/","title":{"rendered":"\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u9001\u6837\u524d\uff0c\u628a\u6837\u672c\u3001\u5206\u6790\u548c\u4ea4\u4ed8\u95ee\u6e05\u695a"},"content":{"rendered":"<p>\u51b3\u5b9a\u505a\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u540e\uff0c\u9879\u76ee\u5f80\u5f80\u4f1a\u8fdb\u5165\u201c\u9009\u5e73\u53f0\u3001\u95ee\u62a5\u4ef7\u3001\u51c6\u5907\u9001\u6837\u201d\u7684\u8282\u594f\u3002\u4f46\u9001\u6837\u524d\u8fd8\u6709\u4e00\u4ef6\u4e8b\u503c\u5f97\u5148\u505a\uff1a\u628a<strong>\u6837\u672c\u80fd\u6d4b\u4ec0\u4e48\u3001\u5206\u6790\u8981\u56de\u7b54\u4ec0\u4e48\u3001\u6700\u540e\u4ea4\u4ed8\u4ec0\u4e48<\/strong>\u653e\u5728\u4e00\u8d77\u6838\u5bf9\u3002<\/p>\n<p>\u4f8b\u5982\uff0c\u82e5\u60f3\u6bd4\u8f83\u80bf\u7624\u4e2d\u5fc3\u4e0e\u4fb5\u88ad\u8fb9\u7f18\uff0c\u5207\u7247\u662f\u5426\u540c\u65f6\u8986\u76d6\u8fd9\u4e24\u4e2a\u533a\u57df\uff0c\u5c31\u76f4\u63a5\u5f71\u54cd\u540e\u7eed\u5206\u6790\u80fd\u5426\u56de\u7b54\u95ee\u9898\u3002\u4e0b\u9762\u8fd9\u5f20\u6e05\u5355\uff0c\u53ef\u7528\u6765\u4e0e\u5b9e\u9a8c\u548c\u5206\u6790\u56e2\u961f\u9010\u9879\u786e\u8ba4\u3002<\/p>\n<h2>\u9001\u6837\u524d\uff0c\u5148\u6838\u5bf9\u8fd9\u4e94\u9879<\/h2>\n<figure class=\"wp-block-table\">\n<table style=\"display:table;width:100%;min-width:0;table-layout:fixed\">\n<thead>\n<tr>\n<th style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u68c0\u67e5\u9879<\/th>\n<th style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u9700\u8981\u51c6\u5907\u7684\u4fe1\u606f<\/th>\n<th style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u4e3a\u4ec0\u4e48\u8981\u5148\u786e\u8ba4<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u7ec4\u7ec7\u4e0e\u4fdd\u5b58<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u7269\u79cd\u3001\u7ec4\u7ec7\u7c7b\u578b\u3001\u51b7\u51bb\u6216 FFPE\u3001\u4fdd\u5b58\u53ca\u5904\u7406\u8bb0\u5f55<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u51b3\u5b9a\u4e0e\u5177\u4f53\u5e73\u53f0\u3001\u8bd5\u5242\u6d41\u7a0b\u662f\u5426\u517c\u5bb9<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5207\u7247\u4e0e\u533a\u57df<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u4ee3\u8868\u6027\u75c5\u7406\u56fe\u3001\u76ee\u6807\u533a\u57df\u3001\u9762\u79ef\u53ca\u574f\u6b7b\u60c5\u51b5<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u786e\u8ba4\u5207\u7247\u662f\u5426\u8986\u76d6\u8981\u7814\u7a76\u7684\u4f4d\u7f6e<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">RNA \u4e0e\u5f62\u6001\u8d28\u91cf<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5e73\u53f0\u8981\u6c42\u7684\u8d28\u91cf\u62a5\u544a\u3001\u5207\u7247\u5b8c\u6574\u6027\u4e0e\u6210\u50cf\u60c5\u51b5<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u907f\u514d\u53ea\u770b\u4e00\u4e2a RNA \u6570\u503c\u5224\u65ad\u53ef\u7528\u6027<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u6837\u672c\u4e0e\u5206\u7ec4<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u72ec\u7acb\u4e2a\u4f53\u6570\u3001\u5bf9\u7167\u3001\u6279\u6b21\u3001\u76f8\u90bb\u5207\u7247\u5173\u7cfb<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u786e\u5b9a\u54ea\u4e9b\u6bd4\u8f83\u6709\u91cd\u590d\u652f\u6301<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5df2\u6709\u8d44\u6599<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5355\u7ec6\u80de\u53c2\u8003\u3001\u8868\u8fbe\u77e9\u9635\u3001\u75c5\u7406\u7ed3\u679c\u53ca\u5019\u9009\u5047\u8bbe<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5224\u65ad\u54ea\u4e9b\u8bc1\u636e\u53ef\u590d\u7528\u3001\u54ea\u4e9b\u4ecd\u9700\u8865\u5145<\/td>\n<\/tr>\n<\/tbody>\n<\/table><figcaption style=\"margin-top:12px\">\u8868 1\uff5c\u9879\u76ee\u6c9f\u901a\u6e05\u5355\uff0c\u4e0d\u66ff\u4ee3\u5177\u4f53\u5e73\u53f0\u7684\u9001\u6837\u6807\u51c6\u3002<\/figcaption><\/figure>\n<p>RIN\u3001DV200\u3001\u7ec4\u7ec7\u539a\u5ea6\u548c\u8fd0\u8f93\u6761\u4ef6\uff0c\u90fd\u5e94\u6309<strong>\u5b9e\u9645\u5e73\u53f0\u3001\u6837\u672c\u7c7b\u578b\u548c\u8bd5\u5242\u7248\u672c<\/strong>\u786e\u8ba4\uff0c\u4e0d\u80fd\u5957\u7528\u540c\u4e00\u6761\u5408\u683c\u7ebf\u3002\u4ee5 <a href=\"https:\/\/www.10xgenomics.com\/support\/spatial-gene-expression-ffpe\/documentation\/steps\/tissue-prep\/visium-spatial-gene-expression-for-ffpe-tissue-preparation-guide\" target=\"_blank\" rel=\"noopener noreferrer\">Visium FFPE \u5b98\u65b9\u7ec4\u7ec7\u51c6\u5907\u6307\u5357<\/a>\u4e3a\u4f8b\uff0c\u5b83\u660e\u786e\u9650\u5b9a\u9002\u7528\u8bd5\u5242\uff0c\u5e76\u5f3a\u8c03\u7ec4\u7ec7\u5f62\u6001\u3001\u8d34\u9644\u548c RNA \u8d28\u91cf\u3002<\/p>\n<p><strong>\u4e00\u4e2a\u6837\u672c\u91cc\u7684\u5927\u91cf Spot\uff0c\u6216\u540c\u4e00\u4e2a\u4f53\u7684\u591a\u5f20\u76f8\u90bb\u5207\u7247\uff0c\u90fd\u4e0d\u80fd\u66ff\u4ee3\u72ec\u7acb\u751f\u7269\u5b66\u91cd\u590d\u3002<\/strong>\u53ea\u6709\u4e00\u4e2a\u6837\u672c\u65f6\uff0c\u53ef\u4ee5\u63a2\u7d22\u5c40\u90e8\u7ed3\u6784\u3001\u5f62\u6210\u5047\u8bbe\uff1b\u8981\u63a8\u5e7f\u4e3a\u7ec4\u95f4\u89c4\u5f8b\uff0c\u5219\u9700\u8981\u76f8\u5e94\u7684\u7814\u7a76\u8bbe\u8ba1\u652f\u6301\u3002<a href=\"https:\/\/www.nature.com\/articles\/s41467-021-25960-2\" target=\"_blank\" rel=\"noopener noreferrer\">\u5173\u4e8e\u751f\u7269\u5b66\u91cd\u590d\u7684\u7814\u7a76<\/a>\u4e5f\u8bf4\u660e\u4e86\u5ffd\u7565\u4e2a\u4f53\u95f4\u5dee\u5f02\u7684\u7edf\u8ba1\u98ce\u9669\u3002<\/p>\n<h2>\u9001\u6837\u65f6\uff0c\u5c31\u628a\u5206\u6790\u8def\u7ebf\u8bf4\u6e05\u695a<\/h2>\n<figure class=\"wp-block-image size-full\"><a href=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/09\/project-checklist-spatial-20260922.png?ssl=1\" target=\"_blank\" rel=\"noopener noreferrer\" aria-label=\"\u67e5\u770b\u5b8c\u6574\u793a\u610f\u56fe\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/09\/project-checklist-spatial-20260922.png?resize=1200%2C1076&#038;ssl=1\" alt=\"\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u9879\u76ee\u51c6\u5907\u56db\u6b65\u793a\u610f\uff1a\u7814\u7a76\u95ee\u9898\u3001\u6837\u672c\u6761\u4ef6\u3001\u5206\u6790\u8def\u5f84\u3001\u9a8c\u8bc1\u4e0e\u4ea4\u4ed8\" width=\"1200\" height=\"1076\" style=\"max-width:100%;height:auto\" \/><\/a><figcaption style=\"margin-top:12px\">\u56fe 1\uff5c\u4ece\u7814\u7a76\u95ee\u9898\u5230\u4ea4\u4ed8\u7684\u51c6\u5907\u987a\u5e8f\u3002\u6b64\u56fe\u4e3a\u6d41\u7a0b\u793a\u610f\u3002<\/figcaption><\/figure>\n<p>\u201c\u505a\u4e00\u5957\u6807\u51c6\u5206\u6790\u201d\u8fd8\u4e0d\u591f\u5177\u4f53\u3002\u82e5\u76ee\u6807\u662f\u5bfb\u627e\u75c5\u7076\u8fb9\u7f18\u7684\u5019\u9009\u7ec6\u80de\uff0c\u53ef\u4ee5\u5148\u7ea6\u5b9a\uff1a\u6838\u5bf9\u7ec4\u7ec7\u56fe\u50cf\u4e0e\u5750\u6807\uff0c\u68c0\u67e5\u533a\u57df\u8d28\u91cf\uff0c\u5224\u65ad\u7ec6\u80de\u53c2\u8003\u662f\u5426\u9002\u5408\uff0c\u518d\u6bd4\u8f83\u76ee\u6807\u533a\u57df\u5e76\u6574\u7406\u5f85\u9a8c\u8bc1\u7684\u5019\u9009\u3002<\/p>\n<p>\u7ed3\u679c\u89e3\u8bfb\u4e5f\u8981\u56de\u5230\u539f\u95ee\u9898\uff1a\u805a\u7c7b\u662f\u5426\u5bf9\u5e94\u75c5\u7406\u7ed3\u6784\uff1f\u5019\u9009\u4fe1\u53f7\u662f\u5426\u7531\u5c40\u90e8\u8d28\u91cf\u6216\u7ec6\u80de\u7ec4\u6210\u9a71\u52a8\uff1f\u4e0d\u540c\u6837\u672c\u662f\u5426\u652f\u6301\u540c\u4e00\u8d8b\u52bf\uff1f\u7a7a\u95f4\u5171\u5b9a\u4f4d\u80fd\u63d0\u51fa\u7ebf\u7d22\uff0c\u673a\u5236\u7ed3\u8bba\u8fd8\u9700\u8981\u72ec\u7acb\u5b9a\u4f4d\u6216\u529f\u80fd\u5b9e\u9a8c\u3002<\/p>\n<h2>\u59d4\u6258\u5206\u6790\uff0c\u5148\u7ea6\u5b9a\u4ea4\u4ed8\u8fb9\u754c<\/h2>\n<p>\u6bd4\u8f83\u62a5\u4ef7\u65f6\uff0c\u5efa\u8bae\u540c\u65f6\u786e\u8ba4\u56db\u4ef6\u4e8b\uff1a<strong>\u8f93\u5165\u6587\u4ef6\u4e0e\u6837\u672c\u5206\u7ec4\u3001\u5206\u6790\u8303\u56f4\u3001\u53ef\u590d\u67e5\u7684\u7ed3\u679c\u3001\u4fee\u6539\u4e0e\u8865\u5206\u6790\u89c4\u5219\u3002<\/strong>\u4ea4\u4ed8\u53ef\u5305\u62ec QC 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