{"id":65546,"date":"2026-10-08T17:00:00","date_gmt":"2026-10-08T09:00:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=65546"},"modified":"2026-10-06T13:35:25","modified_gmt":"2026-10-06T05:35:25","slug":"reviewer-cell-annotation","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/reviewer-cell-annotation\/","title":{"rendered":"\u5ba1\u7a3f\u4eba\u4e0d\u8ba4\u53ef\u5355\u7ec6\u80de\u6ce8\u91ca\uff1a\u53ea\u8865\u4e00\u5f20 marker \u56fe\u591f\u4e0d\u591f\uff1f"},"content":{"rendered":"<p>\u5ba1\u7a3f\u4eba\u95ee\u201cWhy was cluster 7 annotated as this cell type?\u201d\uff0c\u8865\u4e00\u4e2a FeaturePlot \u53ef\u80fd\u6709\u5e2e\u52a9\uff0c\u4f46\u4e0d\u4e00\u5b9a\u56de\u7b54\u4e86\u95ee\u9898\u3002\u5bf9\u65b9\u60f3\u77e5\u9053\u7684\u901a\u5e38\u4e0d\u662f\u201c\u8fd9\u4e2a\u57fa\u56e0\u6709\u6ca1\u6709\u8868\u8fbe\u201d\uff0c\u800c\u662f\u201c\u4e3a\u4ec0\u4e48\u8fd9\u4e2a\u89e3\u91ca\u6bd4\u5176\u4ed6\u7ec6\u80de\u8eab\u4efd\u66f4\u5408\u7406\u201d\u3002<\/p>\n<p>\u5148\u4e0d\u8981\u6025\u7740\u52a0\u66f4\u591a marker\u3002\u628a\u5b58\u5728\u4e89\u8bae\u7684 cluster \u5355\u72ec\u5217\u51fa\u6765\uff1a\u5f53\u524d\u6807\u7b7e\u662f\u4ec0\u4e48\uff0c\u4e3b\u8981\u5907\u9009\u89e3\u91ca\u662f\u4ec0\u4e48\uff0c\u54ea\u4e9b\u8bc1\u636e\u652f\u6301\uff0c\u54ea\u4e9b\u4fe1\u53f7\u4e0d\u7b26\u5408\u3002\u6807\u7b7e\u8d8a\u7ec6\uff0c\u6240\u9700\u8bc1\u636e\u5c31\u8d8a\u5177\u4f53\u3002\u514d\u75ab\u7ec6\u80de\u5927\u7c7b\u548c\u529f\u80fd\u4e9a\u578b\uff0c\u4e0d\u5e94\u9760\u540c\u6837\u7684\u4e00\u4e24\u4e2a\u57fa\u56e0\u5224\u65ad\u3002<\/p>\n<figure style=\"margin:28px 0\"><a href=\"https:\/\/www.biocloudservice.com\/revision-assistant\/assets\/diagrams\/cell-annotation.svg\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" src=\"https:\/\/www.biocloudservice.com\/revision-assistant\/assets\/diagrams\/cell-annotation.svg\" alt=\"\u7ed9\u4e00\u4e2a\u7ec6\u80de\u6807\u7b7e\uff0c\u7559\u4e00\u6761\u8bc1\u636e\u94fe\uff1a\u770b\u6210\u7ec4\u6807\u5fd7\uff0c\u652f\u6301\u4e0e\u6392\u9664\u8bc1\u636e\u4e00\u8d77\u770b\uff1b\u6392\u67e5\u6280\u672f\u5f02\u5e38\uff0c\u53cc\u7ec6\u80de\u3001\u6c61\u67d3\u4e0e\u4f4e\u8d28\u91cf\uff1b\u6838\u5bf9\u6837\u672c\u5206\u5e03\uff0c\u662f\u5426\u7531\u5355\u4e00\u6837\u672c\u4e3b\u5bfc\uff1b\u7ed9\u5408\u9002\u7684\u6807\u7b7e\uff0c\u8bc1\u636e\u4e0d\u8db3\uff0c\u4e0d\u786c\u5b9a\u4e9a\u578b\" width=\"960\" height=\"610\" loading=\"lazy\" style=\"width:100%;height:auto;border-radius:16px\"><\/a><figcaption style=\"font-size:13px;color:#637982\">\u7ed9\u4e00\u4e2a\u7ec6\u80de\u6807\u7b7e\uff0c\u7559\u4e00\u6761\u8bc1\u636e\u94fe\u3002\u65b9\u6cd5\u793a\u610f\uff0c\u4e0d\u662f\u5b9e\u9a8c\u7ed3\u679c\u3002\u70b9\u51fb\u53ef\u653e\u5927\u3002<\/figcaption><\/figure>\n<h2 id=\"section-2\">\u4e00\u5f20\u6ce8\u91ca\u8bc1\u636e\u8868\uff0c\u6bd4\u5806\u5341\u5f20\u56fe\u66f4\u5bb9\u6613\u8bfb<\/h2>\n<div class=\"revision-table\" style=\"margin:24px 0\" role=\"region\" aria-label=\"\u53ef\u76f4\u63a5\u7167\u7740\u6574\u7406\u7684\u6ce8\u91ca\u8bc1\u636e\u8868\uff08\u5b57\u6bb5\u793a\u610f\uff0c\u65e0\u865a\u6784\u5b9e\u9a8c\u6570\u503c\uff09\" tabindex=\"0\">\n<table style=\"width:100%;min-width:570px;border-collapse:collapse;font-size:14px;line-height:1.8\">\n<caption style=\"text-align:left;padding:0 0 12px;font-weight:600\">\u53ef\u76f4\u63a5\u7167\u7740\u6574\u7406\u7684\u6ce8\u91ca\u8bc1\u636e\u8868\uff08\u5b57\u6bb5\u793a\u610f\uff0c\u65e0\u865a\u6784\u5b9e\u9a8c\u6570\u503c\uff09<\/caption>\n<thead>\n<tr>\n<th scope=\"col\" style=\"padding:14px;text-align:left;background:#173d4a;color:white;border:1px solid #d6e3e2\">\u8bb0\u5f55\u9879<\/th>\n<th scope=\"col\" style=\"padding:14px;text-align:left;background:#173d4a;color:white;border:1px solid #d6e3e2\">\u5e94\u5199\u5165\u4ec0\u4e48<\/th>\n<th scope=\"col\" style=\"padding:14px;text-align:left;background:#173d4a;color:white;border:1px solid #d6e3e2\">\u770b\u5230\u4ec0\u4e48\u8981\u8c28\u614e<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u652f\u6301\u6807\u5fd7<\/td>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u591a\u4e2a\u76f8\u5173\u6807\u5fd7\u7684\u8868\u8fbe\u6bd4\u4f8b\u3001\u5f3a\u5ea6\u4e0e\u76f8\u5bf9\u7279\u5f02\u6027<\/td>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u53ea\u6709\u4e00\u4e2a\u5e7f\u6cdb\u8868\u8fbe\u7684\u57fa\u56e0\u652f\u6301<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u76f8\u53cd\u8bc1\u636e<\/td>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u5907\u9009\u8c31\u7cfb\u6807\u5fd7\u53ca\u5b83\u4eec\u7684\u5206\u5e03<\/td>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u4e24\u7c7b\u4e0d\u76f8\u5bb9\u8c31\u7cfb\u4fe1\u53f7\u540c\u65f6\u5f88\u5f3a<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u6837\u672c\u6765\u6e90<\/td>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u5404\u72ec\u7acb\u6837\u672c\u5728\u8be5\u7fa4\u4e2d\u7684\u7ec6\u80de\u6570\u4e0e\u6bd4\u4f8b<\/td>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u51e0\u4e4e\u5168\u90e8\u6765\u81ea\u540c\u4e00\u4e2a\u6837\u672c<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u53c2\u8003\u8bc1\u636e<\/td>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u53c2\u8003\u6570\u636e\u6765\u6e90\u3001\u7ec4\u7ec7\u80cc\u666f\u548c\u6620\u5c04\u7f6e\u4fe1\u60c5\u51b5<\/td>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u53c2\u8003\u5e93\u6ca1\u6709\u8986\u76d6\u76ee\u6807\u72b6\u6001\u5374\u5f3a\u884c\u8d34\u6807\u7b7e<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u6700\u7ec8\u547d\u540d<\/td>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u6807\u7b7e\u7c92\u5ea6\u4e0e\u4e0d\u786e\u5b9a\u6027\u8bf4\u660e<\/td>\n<td style=\"padding:14px;border:1px solid #d6e3e2;vertical-align:top\">\u8bc1\u636e\u4e00\u822c\uff0c\u540d\u5b57\u5374\u7cbe\u786e\u5230\u529f\u80fd\u72b6\u6001<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>\u8fd9\u91cc\u4e0d\u63d0\u4f9b\u4e00\u5957\u201c\u5168\u7ec4\u7ec7\u901a\u7528 marker \u8868\u201d\uff0c\u56e0\u4e3a\u90a3\u6837\u53cd\u800c\u5bb9\u6613\u8bef\u5bfc\u3002\u4e0d\u540c\u7ec4\u7ec7\u3001\u7269\u79cd\u3001\u72b6\u6001\u3001\u5e73\u53f0\u548c\u8d28\u63a7\u80cc\u666f\u4e0b\uff0c\u540c\u4e00\u4e2a\u6807\u5fd7\u7684\u89e3\u91ca\u53ef\u80fd\u4e0d\u540c\u3002\u6ce8\u91ca\u8868\u91cc\u5e94\u5199\u660e\u4f60\u7684\u5b9e\u9645\u80cc\u666f\u548c\u53c2\u8003\u51fa\u5904\u3002<\/p>\n<h2 id=\"section-5\">\u5148\u6392\u9664\u4e09\u4e2a\u5e38\u89c1\u8bef\u4f1a<\/h2>\n<p>\u4e00\u662f\u628a\u73af\u5883 RNA \u5f53\u6210\u8eab\u4efd\u4f9d\u636e\u3002\u67d0\u7c7b\u9ad8\u4e30\u5ea6\u8f6c\u5f55\u672c\u5728\u5176\u4ed6\u7ec6\u80de\u91cc\u51fa\u73b0\uff0c\u4e0d\u80fd\u76f4\u63a5\u8bc1\u660e\u5f62\u6210\u4e86\u65b0\u4e9a\u7fa4\u3002\u4e8c\u662f\u5ffd\u7565\u53cc\u7ec6\u80de\u53ef\u80fd\u6027\uff1b\u4e24\u6761\u8c31\u7cfb\u540c\u65f6\u51fa\u73b0\u65f6\uff0c\u8981\u628a\u53cc\u7ec6\u80de\u68c0\u6d4b\u548c\u539f\u59cb\u8d28\u63a7\u4fe1\u606f\u4e00\u8d77\u68c0\u67e5\u3002\u4e09\u662f\u628a\u805a\u7c7b\u5206\u5f97\u66f4\u7ec6\uff0c\u5f53\u6210\u53d1\u73b0\u4e86\u66f4\u591a\u771f\u5b9e\u7ec6\u80de\u7c7b\u578b\u3002\u5206\u8fa8\u7387\u53d8\u5316\u540e\u7684\u7ec6\u7fa4\uff0c\u4ecd\u9700\u8981\u751f\u7269\u5b66\u8bc1\u636e\u3002<\/p>\n<p>\u7528\u53c2\u8003\u6620\u5c04\u53ef\u4ee5\u8865\u5145\u4f9d\u636e\uff0c\u4f46\u4e0d\u8981\u628a\u81ea\u52a8\u6807\u7b7e\u5f53\u88c1\u5224\u3002\u4fdd\u7559\u6a21\u578b\u6216\u65b9\u6cd5\u540d\u79f0\u3001\u53c2\u8003\u96c6\u7248\u672c\u3001\u6620\u5c04\u7ed3\u679c\u548c\u4e0d\u786e\u5b9a\u7684\u7ec6\u80de\uff1b\u81ea\u52a8\u7ed3\u679c\u4e0e\u4eba\u5de5\u8bc1\u636e\u51b2\u7a81\u65f6\uff0c\u5148\u89e3\u91ca\u51b2\u7a81\uff0c\u4e0d\u662f\u6311\u4e00\u4e2a\u66f4\u7b26\u5408\u6545\u4e8b\u7684\u6807\u7b7e\u3002<\/p>\n<h2 id=\"section-8\">\u8865\u56fe\u5e94\u8be5\u8ba9\u8bfb\u8005\u89e3\u51b3\u4ec0\u4e48\u7591\u95ee<\/h2>\n<p>\u53ef\u4ee5\u7528\u4e00\u5f20 DotPlot \u6c47\u603b\u6210\u7ec4\u6807\u5fd7\uff0c\u518d\u914d\u4e00\u5f20\u6309\u6837\u672c\u62c6\u5206\u7684\u5206\u5e03\u56fe\u3002\u5fc5\u8981\u65f6\u5c55\u793a\u5907\u9009\u6807\u7b7e\u7684\u652f\u6301\u4e0e\u6392\u9664\u8bc1\u636e\u3002\u56fe\u4f8b\u5199\u6e05\u989c\u8272\u3001\u70b9\u5927\u5c0f\u3001\u4f7f\u7528\u7684\u6570\u636e\u5c42\u4e0e\u5206\u7ec4\u5355\u4f4d\u3002\u82e5\u7ecf\u8fc7\u6574\u5408\uff0c\u68c0\u67e5\u539f\u59cb\u6216\u9002\u5f53\u5f52\u4e00\u5316\u8868\u8fbe\u4e2d\u7684\u5173\u952e\u4fe1\u53f7\uff0c\u4e0d\u8981\u4ec5\u51ed\u4e8c\u7ef4\u5d4c\u5165\u4f4d\u7f6e\u547d\u540d\u3002<\/p>\n<p>\u67d0\u4e2a\u4e9a\u7fa4\u4ecd\u8bf4\u4e0d\u6e05\uff0c\u53ef\u4ee5\u4fdd\u7559\u4e3a\u5927\u7c7b\u3001\u6807\u8bb0\u4e3a\u5f85\u5b9a\uff0c\u6216\u4e0d\u5bf9\u5b83\u4f5c\u8fc7\u7ec6\u7684\u529f\u80fd\u89e3\u91ca\u3002\u8fd9\u4e0d\u662f\u201c\u5206\u6790\u6ca1\u505a\u597d\u201d\u7684\u540c\u4e49\u8bcd\uff0c\u800c\u662f\u907f\u514d\u628a\u6709\u9650\u8bc1\u636e\u8bf4\u8fc7\u5934\u3002\u82e5\u91cd\u6ce8\u91ca\u6539\u53d8\u4e86\u540e\u7eed\u7ec6\u80de\u6bd4\u4f8b\u3001\u5dee\u5f02\u8868\u8fbe\u6216\u901a\u8baf\u5206\u6790\uff0c\u8981\u540c\u6b65\u66f4\u65b0\u8fd9\u4e9b\u7ed3\u679c\u3002<\/p>\n<blockquote style=\"border-left:3px solid #008078;background:#f0f7f6;padding:20px 24px\">\n<p lang=\"en\">We have re-examined the annotation of cluster [X] using [marker panels], [sample-level distribution] and [reference mapping, if performed]. The supporting and conflicting evidence is summarized in [table\/figure]. We have renamed this population as [supported label] and revised [affected downstream analyses]. The finer subtype remains uncertain and is not used to support [unsupported interpretation].<\/p>\n<\/blockquote>\n<p style=\"font-size:13px;color:#61747c\">\u8fd9\u662f\u53ef\u4fee\u6539\u7684\u5199\u4f5c\u6846\u67b6\uff0c\u4e0d\u662f\u5bf9\u4f60\u7684\u7814\u7a76\u7ed3\u679c\u7684\u9648\u8ff0\uff1b\u672a\u5b8c\u6210\u7684\u5de5\u4f5c\u4e0d\u5f97\u5199\u6210\u5df2\u5b8c\u6210\u3002<\/p>\n<p>\u63d0\u4ea4\u524d\u8bf7\u8ba9\u4e00\u4e2a\u6ca1\u53c2\u4e0e\u5206\u6790\u7684\u4eba\u53ea\u770b\u8fd9\u5f20\u8bc1\u636e\u8868\uff1a\u4ed6\u662f\u5426\u80fd\u77e5\u9053\u4f60\u4e3a\u4ec0\u4e48\u8fd9\u6837\u547d\u540d\u3001\u53c8\u4e3a\u4ec0\u4e48\u6ca1\u6709\u547d\u540d\u5f97\u66f4\u7ec6\uff1f\u5982\u679c\u4e0d\u80fd\uff0c\u7f3a\u7684\u5f80\u5f80\u662f\u89e3\u91ca\u94fe\u6761\uff0c\u4e0d\u662f\u4e00\u5f20\u66f4\u9c9c\u8273\u7684 UMAP\u3002<\/p>\n<aside style=\"background:#edf7f4;border:1px solid #c7e0da;border-radius:14px;padding:24px;margin:34px 0\">\n<h2 style=\"margin-top:0\">\u628a\u4f60\u624b\u4e0a\u7684\u90a3\u6761\u610f\u89c1\uff0c\u6574\u7406\u6210\u5de5\u4f5c\u5355<\/h2>\n<p>\u5982\u679c\u8fd8\u62ff\u4e0d\u51c6\u4ece\u54ea\u91cc\u5f00\u59cb\uff0c\u53ef\u4ee5\u4f7f\u7528\u4e91\u751f\u4fe1\u7684<a href=\"https:\/\/www.biocloudservice.com\/revision-assistant\/?topic=annotation&amp;from=cell-annotation\">\u5ba1\u7a3f\u610f\u89c1\u8fd4\u4fee\u52a9\u624b<\/a>\u3002\u4e0d\u7528\u767b\u5f55\u6216\u7559\u7535\u8bdd\uff0c\u5148\u5f97\u5230\u5904\u7406\u8def\u5f84\u3001\u6750\u6599\u6e05\u5355\u548c\u53ef\u7f16\u8f91\u7684\u56de\u590d\u6846\u67b6\uff0c\u590d\u5236\u548c\u4e0b\u8f7d\u4e5f\u514d\u8d39\u3002<\/p>\n<p>\u9700\u8981\u4eba\u4e00\u8d77\u5224\u65ad\u65f6\uff0c\u518d\u81ea\u613f\u7533\u8bf7\u6280\u672f\u4eba\u5458\u590d\u6838\u3002\u6d89\u53ca\u5206\u6790\u670d\u52a1\u7684\u8303\u56f4\u4e0e\u8d39\u7528\uff0c\u53e6\u884c\u6c9f\u901a\u786e\u8ba4\uff0c\u4e0d\u627f\u8bfa\u5f55\u7528\u7ed3\u679c\u3002<\/p>\n<p><a href=\"https:\/\/www.biocloudservice.com\/revision-assistant\/?topic=annotation&amp;from=cell-annotation\" style=\"display:inline-block;background:#007d7b;color:white;padding:11px 20px;border-radius:8px;text-decoration:none\">\u5148\u68b3\u7406\u8fd9\u4e00\u6761\u5ba1\u7a3f\u610f\u89c1 \u2192<\/a><\/p>\n<\/aside>\n<h2>\u53c2\u8003\u4e0e\u66f4\u65b0<\/h2>\n<p>\u672c\u6587\u7684\u5904\u7406\u6b65\u9aa4\u4e3a\u7f16\u8f91\u6574\u7406\u7684\u5b9e\u8df5\u5efa\u8bae\uff0c\u9700\u7ed3\u5408\u7814\u7a76\u8bbe\u8ba1\u5224\u65ad\uff1b\u793a\u4f8b\u4e0d\u662f\u5ba2\u6237\u6848\u4f8b\uff0c\u8868\u683c\u4e0d\u5305\u542b\u865a\u6784\u5b9e\u6d4b\u6570\u636e\u3002<\/p>\n<ul>\n<li><a href=\"https:\/\/satijalab.org\/seurat\/articles\/integration_introduction\" target=\"_blank\" rel=\"noopener noreferrer\">Seurat\uff1a\u6574\u5408\u4e0e\u7ec6\u80de\u72b6\u6001\u89e3\u91ca\u7684\u5b9e\u73b0\u80cc\u666f<\/a><\/li>\n<\/ul>\n<p style=\"font-size:13px;color:#637982\">\u4e91\u751f\u4fe1 \u00b7 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