{"id":65809,"date":"2026-10-12T09:00:00","date_gmt":"2026-10-12T01:00:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=65809"},"modified":"2026-10-10T12:13:36","modified_gmt":"2026-10-10T04:13:36","slug":"star-alignment-server-rental-guide","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/star-alignment-server-rental-guide\/","title":{"rendered":"STAR \u6bd4\u5bf9\u4e3a\u4ec0\u4e48\u603b\u5361\u4f4f\uff1f\u771f\u6b63\u7684\u74f6\u9888\uff0c\u5f80\u5f80\u4e0d\u5728\u547d\u4ee4"},"content":{"rendered":"<p>FASTQ \u5df2\u7ecf\u4e0b\u8f7d\u597d\u4e86\uff0cSTAR \u547d\u4ee4\u4e5f\u7167\u7740\u6559\u7a0b\u5199\u5b8c\u4e86\uff0c\u4efb\u52a1\u5374\u8fdf\u8fdf\u8dd1\u4e0d\u8d77\u6765\uff1a\u5efa\u7d22\u5f15\u65f6\u8fdb\u7a0b\u88ab\u6740\u6389\uff0c\u591a\u5f00\u7ebf\u7a0b\u540e\u7535\u8111\u8d8a\u6765\u8d8a\u5361\uff0c\u8f93\u51fa\u6392\u5e8f BAM \u65f6\u78c1\u76d8\u53c8\u7a81\u7136\u544a\u6025\u3002<\/p>\n<p>\u9047\u5230\u8fd9\u4e9b\u95ee\u9898\uff0c\u5f88\u591a\u4eba\u7684\u7b2c\u4e00\u53cd\u5e94\u662f\u68c0\u67e5\u53c2\u6570\u3002\u53ef\u5bf9 STAR \u6765\u8bf4\uff0c<strong>\u547d\u4ee4\u53ea\u662f\u5165\u53e3\uff0c\u771f\u6b63\u51b3\u5b9a\u4efb\u52a1\u80fd\u5426\u7a33\u5b9a\u5b8c\u6210\u7684\uff0c\u662f CPU\u3001\u5185\u5b58\u3001\u5b58\u50a8\u3001I\/O \u548c\u8fd0\u884c\u73af\u5883\u80fd\u4e0d\u80fd\u5f7c\u6b64\u5339\u914d\u3002<\/strong><\/p>\n<p>\u8fd9\u4e5f\u662f\u4e3a\u4ec0\u4e48\uff0c\u6b63\u5f0f\u5904\u7406\u4eba\u6216\u5c0f\u9f20 RNA-seq \u6570\u636e\u3001\u6279\u91cf\u6837\u672c\u6216\u8005\u6709\u660e\u786e\u4ea4\u4ed8\u65f6\u95f4\u7684\u4efb\u52a1\u65f6\uff0c\u4e00\u53f0\u6309\u4efb\u52a1\u914d\u7f6e\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668\uff0c\u5f80\u5f80\u6bd4\u53cd\u590d\u6298\u817e\u672c\u5730\u7535\u8111\u66f4\u5408\u9002\u3002<\/p>\n<h2>STAR \u5f88\u5feb\uff0c\u4e3a\u4ec0\u4e48\u4ecd\u7136\u9700\u8981\u8f83\u591a\u8d44\u6e90\uff1f<\/h2>\n<p>STAR \u5168\u79f0\u662f <strong>Spliced Transcripts Alignment to a Reference<\/strong>\uff0c\u4e3b\u8981\u7528\u4e8e\u628a RNA-seq reads \u6bd4\u5bf9\u5230\u53c2\u8003\u57fa\u56e0\u7ec4\uff0c\u5e76\u8bc6\u522b\u8de8\u5916\u663e\u5b50\u8fde\u63a5\u7684\u526a\u63a5\u6bd4\u5bf9\u3002<\/p>\n<p>\u5178\u578b\u6d41\u7a0b\u53ef\u4ee5\u6982\u62ec\u6210\u4e24\u6b65\uff1a\u5148\u6839\u636e\u53c2\u8003\u57fa\u56e0\u7ec4\u548c\u6ce8\u91ca\u6587\u4ef6\u751f\u6210 genome index\uff0c\u518d\u8bfb\u53d6 FASTQ \u5b8c\u6210\u6bd4\u5bf9\uff0c\u8f93\u51fa BAM\u3001\u526a\u63a5\u4f4d\u70b9\u548c\u8fd0\u884c\u65e5\u5fd7\u7b49\u7ed3\u679c\u3002<\/p>\n<p>STAR \u7684\u6838\u5fc3\u8bbe\u8ba1\u8ffd\u6c42\u9ad8\u901f\u6bd4\u5bf9\u3002<a href=\"https:\/\/academic.oup.com\/bioinformatics\/article\/29\/1\/15\/272537\" target=\"_blank\" rel=\"noopener noreferrer\">STAR \u539f\u59cb\u8bba\u6587<\/a>\u4ecb\u7ecd\u4e86\u57fa\u4e8e\u672a\u538b\u7f29 suffix array \u7684\u68c0\u7d22\u548c\u62fc\u63a5\u65b9\u5f0f\u3002\u901f\u5ea6\u4f18\u52bf\u80cc\u540e\uff0c\u4e5f\u610f\u5473\u7740\u53c2\u8003\u7d22\u5f15\u9700\u8981\u5360\u7528\u8f83\u5927\u7684\u5185\u5b58\uff1b\u6bd4\u5bf9\u8fc7\u7a0b\u4e2d\u8fd8\u8981\u6301\u7eed\u8bfb\u53d6 FASTQ\u3001\u5199\u5165\u7ed3\u679c\uff0c\u5e76\u53ef\u80fd\u8fdb\u884c BAM \u6392\u5e8f\u3002<\/p>\n<p><strong>STAR \u53ef\u4ee5\u5f88\u5feb\uff0c\u4f46\u524d\u63d0\u662f\u5148\u7ed9\u5b83\u8db3\u591f\u4e14\u76f8\u4e92\u5339\u914d\u7684\u8d44\u6e90\u3002<\/strong><\/p>\n<figure class=\"wp-block-image size-full\"><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/star-alignment-server-rental-20261010-star-poster.png?ssl=1\" alt=\"STAR \u6bd4\u5bf9\u4e3a\u4ec0\u4e48\u9700\u8981\u79df\u8d41\u751f\u4fe1\u670d\u52a1\u5668\uff1a\u5185\u5b58\u3001\u78c1\u76d8\u3001\u73af\u5883\u74f6\u9888\u4e0e\u914d\u7f6e\u5efa\u8bae\" loading=\"lazy\" decoding=\"async\"><figcaption>STAR \u4e0d\u662f\u5355\u7eaf\u589e\u52a0\u7ebf\u7a0b\u5c31\u80fd\u89e3\u51b3\u7684\u4efb\u52a1\u3002\u5185\u5b58\u3001\u78c1\u76d8\u3001I\/O \u548c\u73af\u5883\u4e2d\u53ea\u8981\u6709\u4e00\u4e2a\u73af\u8282\u8ddf\u4e0d\u4e0a\uff0c\u6574\u6761\u6d41\u7a0b\u90fd\u53ef\u80fd\u88ab\u62d6\u6162\u6216\u4e2d\u65ad\u3002<\/figcaption><\/figure>\n<h2>\u7b2c\u4e00\u4e2a\u74f6\u9888\uff1a\u7d22\u5f15\u80fd\u52a0\u8f7d\uff0c\u4e0d\u7b49\u4e8e\u6d41\u7a0b\u80fd\u8dd1\u5b8c<\/h2>\n<p>STAR \u8fd0\u884c\u524d\uff0c\u9700\u8981\u628a\u53c2\u8003\u57fa\u56e0\u7ec4\u7d22\u5f15\u52a0\u8f7d\u5230\u5185\u5b58\u3002\u5bf9\u4e8e\u4eba\u3001\u5c0f\u9f20\u7b49\u5178\u578b\u54fa\u4e73\u52a8\u7269\u57fa\u56e0\u7ec4\uff0c\u8fd9\u4e00\u6b65\u672c\u8eab\u5c31\u53ef\u80fd\u5360\u7528\u51e0\u5341 GB \u5185\u5b58\u3002<\/p>\n<p><a href=\"https:\/\/github.com\/alexdobin\/STAR\" target=\"_blank\" rel=\"noopener noreferrer\">STAR \u5b98\u65b9\u624b\u518c<\/a>\u5728\u4ecb\u7ecd\u5171\u4eab\u5185\u5b58\u65f6\uff0c\u4ee5\u4eba\u7c7b\u57fa\u56e0\u7ec4\u7d22\u5f15\u7ea6 31 GB \u4e3a\u793a\u4f8b\u3002\u5b9e\u9645\u4efb\u52a1\u8fd8\u8981\u7ed9\u64cd\u4f5c\u7cfb\u7edf\u3001\u8f93\u5165\u89e3\u538b\u3001BAM \u6392\u5e8f\u548c\u5176\u4ed6\u8fdb\u7a0b\u7559\u51fa\u4f59\u91cf\u3002\u56e0\u6b64\uff0c<strong>\u201c\u7d22\u5f15\u52c9\u5f3a\u88c5\u5f97\u4e0b\u201d\u5e76\u4e0d\u7b49\u4e8e\u201c\u6574\u5957\u6d41\u7a0b\u80fd\u7a33\u5b9a\u8dd1\u5b8c\u201d\u3002<\/strong><\/p>\n<p>\u672c\u5730\u7535\u8111\u5e38\u89c1\u7684\u95ee\u9898\u662f\uff1a\u6807\u79f0\u5185\u5b58\u770b\u8d77\u6765\u591f\uff0c\u4f46\u6d4f\u89c8\u5668\u3001\u529e\u516c\u8f6f\u4ef6\u548c\u5176\u4ed6\u8fdb\u7a0b\u5df2\u7ecf\u5360\u6389\u4e00\u90e8\u5206\uff1b\u4efb\u52a1\u8fdb\u5165\u6392\u5e8f\u9636\u6bb5\u540e\u5185\u5b58\u518d\u6b21\u51b2\u9ad8\uff0c\u6700\u7ec8\u51fa\u73b0 <code>Killed<\/code>\u3001out of memory \u6216\u957f\u65f6\u95f4\u65e0\u54cd\u5e94\u3002<\/p>\n<h2>\u7b2c\u4e8c\u4e2a\u74f6\u9888\uff1a\u7ebf\u7a0b\u6570\u4e0d\u80fd\u8131\u79bb\u5185\u5b58\u548c\u78c1\u76d8\u5355\u72ec\u770b<\/h2>\n<p>STAR \u652f\u6301\u901a\u8fc7 <code>--runThreadN<\/code> \u4f7f\u7528\u591a\u4e2a\u7ebf\u7a0b\u3002\u589e\u52a0\u7ebf\u7a0b\u540e\uff0c\u5f88\u591a\u573a\u666f\u4e0b\u6bd4\u5bf9\u4f1a\u66f4\u5feb\uff0c\u4f46\u5b83\u5e76\u4e0d\u662f\u8d8a\u5927\u8d8a\u597d\u3002<\/p>\n<p>\u5982\u679c\u5185\u5b58\u4e0d\u8db3\uff0c\u7ebf\u7a0b\u518d\u591a\u4e5f\u4f1a\u5931\u8d25\uff1b\u5982\u679c\u78c1\u76d8\u8bfb\u5199\u8ddf\u4e0d\u4e0a\uff0cCPU \u4f1a\u7b49\u5f85 I\/O\uff1b\u5982\u679c\u540c\u4e00\u53f0\u673a\u5668\u540c\u65f6\u8dd1\u591a\u4e2a\u6837\u672c\uff0c\u5185\u5b58\u3001CPU \u548c\u4e34\u65f6\u7a7a\u95f4\u8fd8\u4f1a\u4e92\u76f8\u4e89\u62a2\u3002<\/p>\n<p>\u5408\u7406\u7684\u987a\u5e8f\u5e94\u8be5\u662f\uff1a<\/p>\n<ol>\n<li>\u5148\u4fdd\u8bc1\u53c2\u8003\u7d22\u5f15\u548c\u8fd0\u884c\u8fc7\u7a0b\u6709\u8db3\u591f\u5185\u5b58\uff1b<\/li>\n<li>\u518d\u6839\u636e\u6837\u672c\u6570\u3001\u5e76\u53d1\u6570\u548c\u671f\u671b\u5b8c\u6210\u65f6\u95f4\u914d\u7f6e CPU\uff1b<\/li>\n<li>\u6700\u540e\u786e\u8ba4\u5b58\u50a8\u4ecb\u8d28\u3001\u53ef\u7528\u7a7a\u95f4\u548c\u4e34\u65f6\u76ee\u5f55\u80fd\u5426\u8ddf\u4e0a\u8bfb\u5199\u538b\u529b\u3002<\/li>\n<\/ol>\n<p>\u6240\u4ee5\uff0c\u79df\u670d\u52a1\u5668\u65f6\u4e0d\u8981\u53ea\u95ee\u201c\u6709\u591a\u5c11\u6838\u201d\uff0c\u8fd8\u8981\u540c\u65f6\u95ee\uff1a<strong>\u8fd9\u4e9b\u6838\u914d\u4e86\u591a\u5c11\u5185\u5b58\uff1f\u5e95\u5c42\u662f\u4ec0\u4e48\u5b58\u50a8\uff1f\u8ba1\u5212\u540c\u65f6\u8dd1\u51e0\u4e2a\u6837\u672c\uff1f<\/strong><\/p>\n<h2>\u7b2c\u4e09\u4e2a\u74f6\u9888\uff1aFASTQ \u4e0d\u662f\u552f\u4e00\u9700\u8981\u5360\u7a7a\u95f4\u7684\u6587\u4ef6<\/h2>\n<p>\u5f88\u591a\u4eba\u53ea\u6309\u539f\u59cb FASTQ \u5927\u5c0f\u4f30\u7b97\u78c1\u76d8\uff0c\u4efb\u52a1\u8dd1\u5230\u4e00\u534a\u624d\u53d1\u73b0\u7a7a\u95f4\u4e0d\u591f\u3002\u5b8c\u6574\u5de5\u4f5c\u76ee\u5f55\u901a\u5e38\u8fd8\u8981\u5bb9\u7eb3\u53c2\u8003\u57fa\u56e0\u7ec4\u4e0e\u6ce8\u91ca\u3001STAR genome index\u3001\u4e34\u65f6\u6587\u4ef6\u3001\u6392\u5e8f\u524d\u540e\u7684 BAM\u3001\u526a\u63a5\u4f4d\u70b9\u8868\u3001\u65e5\u5fd7\uff0c\u4ee5\u53ca\u4e3a\u91cd\u8dd1\u6216\u590d\u6838\u4fdd\u7559\u7684\u526f\u672c\u3002<\/p>\n<p>STAR \u5b98\u65b9\u624b\u518c\u8fd8\u63d0\u9192\uff1a\u4e3a\u5178\u578b\u54fa\u4e73\u52a8\u7269\u57fa\u56e0\u7ec4\u751f\u6210\u7d22\u5f15\u65f6\uff0c\u6587\u4ef6\u7cfb\u7edf\u5e94\u81f3\u5c11\u6709 100 GB \u53ef\u7528\u7a7a\u95f4\u3002\u8fd9\u4e2a\u6570\u5b57\u4e0d\u662f\u5b8c\u6574\u9879\u76ee\u7684\u901a\u7528\u914d\u7f6e\uff0c\u800c\u662f\u5728\u63d0\u9192\u6211\u4eec\uff1a<strong>\u53ea\u7ed9\u539f\u59cb\u6570\u636e\u7559\u7a7a\u95f4\uff0c\u8fdc\u8fdc\u4e0d\u591f\u3002<\/strong><\/p>\n<p>\u5bb9\u91cf\u4e4b\u5916\u8fd8\u8981\u770b\u901f\u5ea6\u3002\u8bfb\u53d6\u538b\u7f29 FASTQ\u3001\u6301\u7eed\u5199\u5165 BAM\u3001\u8fdb\u884c\u5750\u6807\u6392\u5e8f\uff0c\u90fd\u4f1a\u4ea7\u751f\u8f83\u91cd\u7684 I\/O\u3002\u78c1\u76d8\u5bb9\u91cf\u591f\u4f46\u901f\u5ea6\u6162\uff0c\u540c\u6837\u4f1a\u8ba9\u591a\u7ebf\u7a0b CPU \u957f\u65f6\u95f4\u7b49\u5f85\u3002<\/p>\n<h2>\u7b2c\u56db\u4e2a\u74f6\u9888\uff1a\u80fd\u542f\u52a8\uff0c\u4e0d\u4ee3\u8868\u80fd\u7a33\u5b9a\u590d\u73b0<\/h2>\n<p>STAR \u5b98\u65b9\u9879\u76ee\u7ed9\u51fa\u7684\u57fa\u7840\u5e73\u53f0\u8981\u6c42\u662f x86-64 \u5904\u7406\u5668\u548c 64 \u4f4d Linux \u6216 macOS\u3002\u5b9e\u9645\u5206\u6790\u8fd8\u5e38\u5e38\u63a5\u7740\u4f7f\u7528 samtools\u3001subread\u3001R\u3001Python \u6216\u4e0b\u6e38\u5b9a\u91cf\u4e0e\u8d28\u63a7\u5de5\u5177\u3002<\/p>\n<p>\u5728\u672c\u5730\u7535\u8111\u4e0a\uff0c\u5e38\u89c1\u963b\u529b\u5305\u62ec\u4f9d\u8d56\u7248\u672c\u4e0d\u4e00\u81f4\u3001PATH \u6216\u6743\u9650\u914d\u7f6e\u9519\u8bef\u3001WSL \u4e0e\u5bbf\u4e3b\u7cfb\u7edf\u4e4b\u95f4\u7684\u8def\u5f84\u548c\u78c1\u76d8\u6027\u80fd\u95ee\u9898\uff0c\u4ee5\u53ca\u4f11\u7720\u3001\u91cd\u542f\u6216\u7ec8\u7aef\u65ad\u5f00\u5bfc\u81f4\u7684\u4efb\u52a1\u4e2d\u65ad\u3002<\/p>\n<p><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/services\/\">\u4e91\u7aef\u8ba1\u7b97\u73af\u5883<\/a>\u7684\u4ef7\u503c\u4e0d\u53ea\u662f\u63d0\u4f9b\u4e00\u7ec4\u786c\u4ef6\u6570\u5b57\u3002\u628a\u8f6f\u4ef6\u7248\u672c\u3001\u53c2\u8003\u7d22\u5f15\u3001\u8f93\u5165\u3001\u547d\u4ee4\u548c\u65e5\u5fd7\u96c6\u4e2d\u5728\u6301\u7eed\u8fd0\u884c\u7684 Linux \u73af\u5883\u4e2d\uff0c\u5bf9\u4e8e\u540e\u7eed\u8865\u6837\u672c\u3001\u91cd\u8dd1\u548c\u56e2\u961f\u534f\u4f5c\u540c\u6837\u91cd\u8981\u3002<\/p>\n<h2>\u5148\u770b\u75c7\u72b6\uff0c\u518d\u5224\u65ad\u7f3a\u7684\u662f\u4ec0\u4e48<\/h2>\n<p>\u770b\u5230\u540c\u4e00\u4e2a\u62a5\u9519\u6216\u53d8\u6162\u73b0\u8c61\u65f6\uff0c\u5148\u786e\u8ba4\u6839\u56e0\uff0c\u518d\u51b3\u5b9a\u662f\u5426\u589e\u52a0\u914d\u7f6e\u3002<\/p>\n<figure class=\"wp-block-table\">\n<table style=\"display:table;width:100%;min-width:0;table-layout:fixed\">\n<thead>\n<tr>\n<th style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5e38\u89c1\u75c7\u72b6<\/th>\n<th style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u66f4\u53ef\u80fd\u7684\u74f6\u9888<\/th>\n<th style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5148\u505a\u4ec0\u4e48<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5efa\u7d22\u5f15\u6216\u52a0\u8f7d\u7d22\u5f15\u65f6\u88ab\u7cfb\u7edf\u6740\u6389<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5185\u5b58\u4e0d\u8db3<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u6838\u5bf9\u5cf0\u503c\u5185\u5b58\u3001\u7cfb\u7edf\u4f59\u91cf\u548c\u7d22\u5f15\u5927\u5c0f<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u7ebf\u7a0b\u5f88\u591a\uff0c\u4f46 CPU \u5229\u7528\u7387\u5ffd\u9ad8\u5ffd\u4f4e<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u78c1\u76d8 I\/O \u6216\u540c\u673a\u4e89\u62a2<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u68c0\u67e5\u78c1\u76d8\u8bfb\u5199\u3001\u5e76\u53d1\u4efb\u52a1\u548c FASTQ \u89e3\u538b<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u751f\u6210\u6392\u5e8f BAM \u65f6\u5931\u8d25<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u6392\u5e8f\u5185\u5b58\u6216\u4e34\u65f6\u7a7a\u95f4\u4e0d\u8db3<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u68c0\u67e5\u6392\u5e8f\u53c2\u6570\u3001\u4e34\u65f6\u76ee\u5f55\u548c\u53ef\u7528\u7a7a\u95f4<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5355\u6837\u672c\u80fd\u8dd1\uff0c\u591a\u6837\u672c\u6392\u961f\u592a\u4e45<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">CPU\u3001\u5e76\u53d1\u6216\u8c03\u5ea6\u4e0d\u8db3<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u6309\u6837\u672c\u6279\u6b21\u8bbe\u8ba1\u5e76\u884c\u65b9\u5f0f<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u6362\u673a\u5668\u6216\u8865\u6837\u672c\u540e\u73af\u5883\u53c8\u62a5\u9519<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u8f6f\u4ef6\u4e0e\u53c2\u8003\u6587\u4ef6\u4e0d\u4e00\u81f4<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u56fa\u5b9a\u7248\u672c\u3001\u7d22\u5f15\u3001\u547d\u4ee4\u548c\u65e5\u5fd7<\/td>\n<\/tr>\n<\/tbody>\n<\/table><figcaption>\u8d44\u6e90\u6392\u67e5\u987a\u5e8f\u7528\u4e8e\u5b9a\u4f4d\u95ee\u9898\uff0c\u4e0d\u4ee3\u8868\u589e\u52a0\u67d0\u4e00\u9879\u914d\u7f6e\u5c31\u4e00\u5b9a\u80fd\u89e3\u51b3\u5168\u90e8\u62a5\u9519\u3002<\/figcaption><\/figure>\n<h2>\u54ea\u4e9b\u60c5\u51b5\u9002\u5408\u672c\u5730\u8dd1\uff0c\u54ea\u4e9b\u60c5\u51b5\u503c\u5f97\u79df\u670d\u52a1\u5668\uff1f<\/h2>\n<p>\u5982\u679c\u53ea\u662f\u5b66\u4e60\u547d\u4ee4\u3001\u5904\u7406\u5c0f\u53c2\u8003\u57fa\u56e0\u7ec4\u6216\u5c11\u91cf\u6d4b\u8bd5\u6570\u636e\uff0c\u800c\u4e14\u672c\u5730\u5df2\u7ecf\u6709\u8db3\u591f\u5185\u5b58\u548c\u9ad8\u901f SSD\uff0c\u672c\u5730\u7535\u8111\u5b8c\u5168\u53ef\u4ee5\u5b8c\u6210\u4efb\u52a1\u3002<\/p>\n<p>\u4f46\u51fa\u73b0\u4ee5\u4e0b\u60c5\u51b5\u65f6\uff0c\u66f4\u5efa\u8bae\u76f4\u63a5\u8bc4\u4f30\u670d\u52a1\u5668\uff1a<\/p>\n<ul>\n<li>\u4eba\u6216\u5c0f\u9f20\u7b49\u8f83\u5927\u53c2\u8003\u57fa\u56e0\u7ec4\uff0c\u9700\u8981\u65b0\u5efa\u6216\u52a0\u8f7d STAR \u7d22\u5f15\uff1b<\/li>\n<li>\u6837\u672c\u8f83\u591a\uff0c\u5e0c\u671b\u6279\u91cf\u8fd0\u884c\u6216\u51cf\u5c11\u6392\u961f\u65f6\u95f4\uff1b<\/li>\n<li>\u9700\u8981\u8f93\u51fa\u6392\u5e8f BAM\u3001\u878d\u5408\u76f8\u5173\u7ed3\u679c\u6216\u4fdd\u7559\u8f83\u591a\u4e2d\u95f4\u6587\u4ef6\uff1b<\/li>\n<li>\u672c\u5730\u53ea\u6709 16 GB \u6216 32 GB \u5185\u5b58\uff0c\u4efb\u52a1\u7ecf\u5e38\u5904\u5728\u8d44\u6e90\u4e34\u754c\u72b6\u6001\uff1b<\/li>\n<li>\u9700\u8981\u8de8\u6210\u5458\u534f\u4f5c\u3001\u590d\u73b0\u540c\u4e00\u73af\u5883\uff0c\u6216\u540e\u7eed\u7ee7\u7eed\u5b9a\u91cf\u4e0e\u5dee\u5f02\u5206\u6790\uff1b<\/li>\n<li>\u9879\u76ee\u6709\u660e\u786e\u65f6\u95f4\u8981\u6c42\uff0c\u4e0d\u9002\u5408\u53cd\u590d\u8bd5\u9519\u548c\u91cd\u8dd1\u3002<\/li>\n<\/ul>\n<p>\u5173\u952e\u4e0d\u662f\u201c\u4e91\u7aef\u4e00\u5b9a\u6bd4\u672c\u5730\u9ad8\u7ea7\u201d\uff0c\u800c\u662f\u770b\uff1a<strong>\u4e3a\u4e00\u6b21\u6216\u4e00\u6279\u4efb\u52a1\u4e34\u65f6\u83b7\u5f97\u5408\u9002\u8d44\u6e90\uff0c\u662f\u5426\u6bd4\u8d2d\u4e70\u3001\u7ef4\u62a4\u4e00\u53f0\u957f\u671f\u95f2\u7f6e\u7684\u9ad8\u914d\u7535\u8111\u66f4\u5408\u7406\u3002<\/strong><\/p>\n<h2>\u79df\u670d\u52a1\u5668\u524d\uff0c\u5148\u628a\u8fd9 6 \u9879\u4fe1\u606f\u8bf4\u6e05\u695a<\/h2>\n<ol>\n<li><strong>\u7269\u79cd\u4e0e\u53c2\u8003\u7248\u672c\uff1a<\/strong>\u4f8b\u5982\u4eba\u3001\u5c0f\u9f20\uff0c\u4f7f\u7528\u54ea\u4e2a genome build \u548c GTF \u6ce8\u91ca\u3002<\/li>\n<li><strong>\u6837\u672c\u6570\u91cf\uff1a<\/strong>\u8fd9\u6b21\u8981\u8dd1\u51e0\u4e2a\u6837\u672c\uff0c\u540e\u7eed\u662f\u5426\u8fd8\u4f1a\u8ffd\u52a0\u3002<\/li>\n<li><strong>\u6570\u636e\u89c4\u6a21\uff1a<\/strong>FASTQ \u603b\u5927\u5c0f\u3001\u6bcf\u4e2a\u6837\u672c\u5927\u81f4 reads \u6570\uff0c\u5355\u7aef\u8fd8\u662f\u53cc\u7aef\u3002<\/li>\n<li><strong>\u8f93\u51fa\u8981\u6c42\uff1a<\/strong>\u662f\u5426\u9700\u8981\u6392\u5e8f BAM\u3001\u57fa\u56e0\u8ba1\u6570\u3001\u526a\u63a5\u4f4d\u70b9\u6216\u878d\u5408\u76f8\u5173\u8f93\u51fa\u3002<\/li>\n<li><strong>\u8fd0\u884c\u65b9\u5f0f\uff1a<\/strong>\u9010\u4e2a\u6837\u672c\u8dd1\uff0c\u8fd8\u662f\u5e0c\u671b\u591a\u4e2a\u6837\u672c\u5e76\u884c\u3002<\/li>\n<li><strong>\u65f6\u95f4\u8981\u6c42\uff1a<\/strong>\u53ea\u662f\u5b66\u4e60\u6d4b\u8bd5\uff0c\u8fd8\u662f\u6709\u660e\u786e\u5206\u6790\u4e0e\u4ea4\u4ed8\u8282\u70b9\u3002<\/li>\n<\/ol>\n<p>\u8fd9\u4e9b\u4fe1\u606f\u51b3\u5b9a\u7684\uff0c\u4e0d\u53ea\u662f CPU \u6838\u6570\uff0c\u8fd8\u5305\u62ec\u5185\u5b58\u3001\u7cfb\u7edf\u76d8\u3001\u6570\u636e\u76d8\u3001\u4e34\u65f6\u7a7a\u95f4\u3001\u8f6f\u4ef6\u73af\u5883\u548c\u5e76\u53d1\u7b56\u7565\u3002<\/p>\n<h2>\u628a\u6570\u636e\u89c4\u6a21\u53d1\u6765\uff0c\u5148\u83b7\u5f97 STAR \u670d\u52a1\u5668\u914d\u7f6e\u5efa\u8bae<\/h2>\n<p>\u5982\u679c\u4f60\u7684 STAR \u4efb\u52a1\u5df2\u7ecf\u51fa\u73b0<strong>\u5185\u5b58\u4e0d\u8db3\u3001\u78c1\u76d8\u544a\u6025\u3001\u73af\u5883\u53cd\u590d\u62a5\u9519\u6216\u591a\u6837\u672c\u6392\u961f\u592a\u4e45<\/strong>\u7b49\u95ee\u9898\uff0c\u53ef\u4ee5\u8054\u7cfb\u4e91\u751f\u4fe1\u4f01\u4e1a\u5fae\u4fe1\u5ba2\u670d\uff0c\u53d1\u9001\u201cSTAR \u914d\u7f6e\u201d\u3002<\/p>\n<p>\u8bf7\u51c6\u5907\uff1a<strong>\u7269\u79cd\u4e0e\u53c2\u8003\u7248\u672c\u3001\u6837\u672c\u6570\u91cf\u3001FASTQ \u603b\u5927\u5c0f\u3001\u5355\u7aef\u6216\u53cc\u7aef\u3001\u9700\u8981\u7684\u8f93\u51fa\uff0c\u4ee5\u53ca\u5e0c\u671b\u5b8c\u6210\u7684\u65f6\u95f4\u3002<\/strong>\u5df2\u6709\u62a5\u9519\u6216\u8fd0\u884c\u65e5\u5fd7\uff0c\u4e5f\u53ef\u4ee5\u4e00\u8d77\u53d1\u6765\u3002<\/p>\n<p>\u6211\u4eec\u4f1a\u5148\u6839\u636e\u4efb\u52a1\u5224\u65ad 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I\/O\u3002\u672c\u6587\u8bf4\u660e\u5e38\u89c1\u75c7\u72b6\u5bf9\u5e94\u7684\u8d44\u6e90\u74f6\u9888\uff0c\u4ee5\u53ca\u79df\u8d41\u751f\u4fe1\u670d\u52a1\u5668\u524d\u5e94\u51c6\u5907\u7684\u4fe1\u606f\u3002<\/p>\n","protected":false},"author":1,"featured_media":65807,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[1,75,72],"tags":[294,293,80,292,291,156,119],"class_list":["post-65809","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-part","category-compute-environment","category-transcriptomics","tag-bam","tag-fastq","tag-rna-seq","tag-star","tag-156","tag-119"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/star-alignment-server-rental-20261010-star-cover.png?fit=1672%2C941&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/65809","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=65809"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/65809\/revisions"}],"predecessor-version":[{"id":65810,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/65809\/revisions\/65810"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/65807"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=65809"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=65809"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=65809"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}