{"id":65816,"date":"2026-10-31T09:00:00","date_gmt":"2026-10-31T01:00:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=65816"},"modified":"2026-10-10T12:33:56","modified_gmt":"2026-10-10T04:33:56","slug":"cell-ranger-batch-samples-concurrency-guide","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/cell-ranger-batch-samples-concurrency-guide\/","title":{"rendered":"\u591a\u4e2a 10x \u6837\u672c\u600e\u4e48\u6279\u91cf\u8dd1 Cell Ranger\uff1f\u5e76\u53d1\u5f00\u592a\u591a\uff0c\u53cd\u800c\u66f4\u6162"},"content":{"rendered":"<p>\u4e00\u4e2a Cell Ranger \u4efb\u52a1\u5df2\u7ecf\u8dd1\u901a\uff0c\u63a5\u4e0b\u6765\u8fd8\u6709\u5341\u51e0\u4e2a\u6837\u672c\u3002\u6700\u76f4\u89c9\u7684\u505a\u6cd5\uff0c\u662f\u628a\u6240\u6709\u4efb\u52a1\u4e00\u8d77\u542f\u52a8\u3002<\/p>\n<p>\u7ed3\u679c\u53ef\u80fd\u6070\u597d\u76f8\u53cd\uff1a\u5185\u5b58\u88ab\u540c\u65f6\u5360\u6ee1\uff0cFASTQ \u548c BAM \u62a2\u540c\u4e00\u5757\u78c1\u76d8\uff0c\u4e34\u65f6\u76ee\u5f55\u8d8a\u6765\u8d8a\u5927\uff0c\u5355\u4e2a\u4efb\u52a1\u53cd\u800c\u6bd4\u539f\u6765\u66f4\u6162\uff0c\u751a\u81f3\u6574\u6279\u4e00\u8d77\u5931\u8d25\u3002<\/p>\n<p><strong>\u591a\u6837\u672c\u6279\u91cf\u8fd0\u884c\u7684\u5173\u952e\uff0c\u4e0d\u662f\u201c\u80fd\u5f00\u51e0\u4e2a\u4efb\u52a1\u201d\uff0c\u800c\u662f\u5148\u7b97\u6e05\u4e00\u4e2a\u4efb\u52a1\u7684\u771f\u5b9e\u8d44\u6e90\uff0c\u518d\u51b3\u5b9a\u5e76\u53d1\u6570\u3002<\/strong><\/p>\n<h2>Cell Ranger \u7684\u5e76\u53d1\uff0c\u4e0d\u80fd\u53ea\u7528 CPU \u6838\u6570\u76f8\u9664<\/h2>\n<p>Cell Ranger \u5728\u5355\u673a\u6a21\u5f0f\u4e0b\u9ed8\u8ba4\u4f7f\u7528\u68c0\u6d4b\u5230\u7684\u5168\u90e8\u6838\u5fc3\u548c\u7ea6 90% \u53ef\u7528\u5185\u5b58\u3002\u5b98\u65b9\u5efa\u8bae\u5728\u5171\u4eab\u73af\u5883\u4e2d\u4f7f\u7528 <code>--localcores<\/code> \u548c <code>--localmem<\/code> \u8bbe\u7f6e\u4e0a\u9650\uff0c\u907f\u514d\u4efb\u52a1\u4e4b\u95f4\u4e92\u76f8\u6324\u5360\u3002<\/p>\n<p>\u5982\u679c\u4e00\u53f0 64 \u6838\u670d\u52a1\u5668\u540c\u65f6\u542f\u52a8\u56db\u4e2a\u4e0d\u8bbe\u4e0a\u9650\u7684\u4efb\u52a1\uff0c\u5b83\u4eec\u4e0d\u4f1a\u81ea\u52a8\u516c\u5e73\u5206\u914d\u8d44\u6e90\u3002\u771f\u6b63\u8981\u540c\u65f6\u8003\u8651\u7684\u662f\uff1a<\/p>\n<ul>\n<li>\u5355\u4efb\u52a1\u5cf0\u503c\u5185\u5b58\uff1b<\/li>\n<li>\u6bcf\u4e2a\u9636\u6bb5\u5b9e\u9645\u4f7f\u7528\u7684\u7ebf\u7a0b\u6570\uff1b<\/li>\n<li>\u591a\u4e2a\u4efb\u52a1\u540c\u65f6\u8bfb FASTQ\u3001\u5199 BAM \u7684 I\/O \u538b\u529b\uff1b<\/li>\n<li>\u6bcf\u4e2a pipestance \u7684\u4e34\u65f6\u548c\u8f93\u51fa\u7a7a\u95f4\uff1b<\/li>\n<li>\u4efb\u52a1\u5931\u8d25\u540e\u662f\u5426\u80fd\u591f\u6e05\u695a\u5b9a\u4f4d\u548c\u91cd\u8dd1\u3002<\/li>\n<\/ul>\n<figure class=\"wp-block-image size-full\"><img data-recalc-dims=\"1\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/cellranger-gwas-series-20261010-cellranger-batch-poster.png?ssl=1\" alt=\"\u591a\u4e2a 10x \u6837\u672c\u6279\u91cf\u8fd0\u884c Cell Ranger\uff1a\u5185\u5b58\u3001I\/O\u3001\u4e34\u65f6\u76d8\u548c\u5e76\u53d1\u8c03\u5ea6\" loading=\"lazy\" decoding=\"async\"><figcaption>\u6279\u91cf\u8fd0\u884c\u4e0d\u662f\u628a\u4efb\u52a1\u5168\u90e8\u6253\u5f00\uff0c\u800c\u662f\u8ba9\u6bcf\u4e2a\u6837\u672c\u83b7\u5f97\u6e05\u695a\u7684\u8d44\u6e90\u4e0a\u9650\u3001\u5de5\u4f5c\u76ee\u5f55\u548c\u8fd0\u884c\u8bb0\u5f55\u3002<\/figcaption><\/figure>\n<h2>\u7b2c\u4e00\u6b65\uff1a\u5148\u7528\u4ee3\u8868\u6837\u672c\u6d4b\u51fa\u5355\u4efb\u52a1\u57fa\u7ebf<\/h2>\n<p>\u5728\u6574\u6279\u542f\u52a8\u524d\uff0c\u5148\u9009\u62e9\u4e00\u4e2a\u5177\u6709\u4ee3\u8868\u6027\u7684\u6837\u672c\u8dd1\u901a\u3002\u4e0d\u8981\u53ea\u8bb0\u5f55\u603b\u8017\u65f6\uff0c\u8fd8\u8981\u8bb0\u5f55\u5cf0\u503c\u5185\u5b58\u3001\u6838\u5fc3\u4f7f\u7528\u3001\u5de5\u4f5c\u76ee\u5f55\u6700\u5927\u5360\u7528\u3001\u6700\u7ec8\u8f93\u51fa\u5927\u5c0f\u548c\u5173\u952e\u65e5\u5fd7\u3002<\/p>\n<p>\u6837\u672c\u95f4\u6570\u636e\u91cf\u5dee\u5f02\u8f83\u5927\u65f6\uff0c\u53ef\u4ee5\u628a\u6837\u672c\u6309\u5c0f\u3001\u4e2d\u3001\u5927\u5206\u7ec4\u3002\u7528\u6700\u5c0f\u6837\u672c\u63a8\u65ad\u6240\u6709\u4efb\u52a1\uff0c\u5f80\u5f80\u4f1a\u4f4e\u4f30\u5927\u6837\u672c\u7684\u8d44\u6e90\uff1b\u76f4\u63a5\u7528\u6700\u5927\u6837\u672c\u4f30\u7b97\u5168\u90e8\u4efb\u52a1\uff0c\u53c8\u53ef\u80fd\u9020\u6210\u4e0d\u5fc5\u8981\u7684\u95f2\u7f6e\u3002<\/p>\n<p>\u8fd9\u4e2a\u57fa\u7ebf\u51b3\u5b9a\u4e86\u540e\u9762\u7684\u5e76\u53d1\u5b89\u6392\uff0c\u4e5f\u51b3\u5b9a\u670d\u52a1\u5668\u9700\u8981\u591a\u5c11\u53ef\u5206\u914d\u8d44\u6e90\u3002<\/p>\n<h2>\u7b2c\u4e8c\u6b65\uff1a\u628a\u5e76\u53d1\u62c6\u6210\u4e09\u9053\u4e0a\u9650<\/h2>\n<p>\u540c\u4e00\u6279\u4efb\u52a1\u80fd\u540c\u65f6\u8fd0\u884c\u591a\u5c11\u4e2a\uff0c\u901a\u5e38\u53d6\u51b3\u4e8e\u4e09\u4e2a\u4e0a\u9650\u4e2d\u6700\u5c0f\u7684\u90a3\u4e2a\uff1a<\/p>\n<ol>\n<li><strong>\u5185\u5b58\u4e0a\u9650\uff1a<\/strong>\u53ef\u7528\u4e8e\u4efb\u52a1\u7684\u603b\u5185\u5b58 \u00f7 \u5355\u4efb\u52a1\u5cf0\u503c\u5185\u5b58\uff1b<\/li>\n<li><strong>CPU \u4e0a\u9650\uff1a<\/strong>\u53ef\u7528\u6838\u5fc3\u6570 \u00f7 \u5355\u4efb\u52a1\u8bbe\u7f6e\u7684 localcores\uff1b<\/li>\n<li><strong>I\/O \u4e0a\u9650\uff1a<\/strong>\u5de5\u4f5c\u76d8\u662f\u5426\u80fd\u627f\u53d7\u591a\u4e2a\u4efb\u52a1\u540c\u65f6\u8bfb\u5199\u548c\u6392\u5e8f\u3002<\/li>\n<\/ol>\n<p>\u5f88\u591a\u6279\u91cf\u4efb\u52a1\u4e0d\u662f\u5361\u5728 CPU\uff0c\u800c\u662f\u5361\u5728\u7b2c\u4e09\u9879\u3002CPU \u770b\u8d77\u6765\u8fd8\u6709\u7a7a\u95f2\uff0c\u78c1\u76d8\u961f\u5217\u5374\u5df2\u7ecf\u62c9\u957f\uff0c\u6b64\u65f6\u7ee7\u7eed\u589e\u52a0\u5e76\u53d1\u53ea\u4f1a\u8ba9\u5168\u90e8\u4efb\u52a1\u4e00\u8d77\u7b49\u5f85\u3002<\/p>\n<h2>\u7b2c\u4e09\u6b65\uff1a\u4e00\u4e2a\u6837\u672c\uff0c\u4e00\u4e2a\u6e05\u695a\u7684\u8f93\u51fa\u8fb9\u754c<\/h2>\n<p>Cell Ranger \u4f1a\u628a\u6bcf\u4e2a\u4efb\u52a1\u7684\u8fd0\u884c\u72b6\u6001\u3001\u4e2d\u95f4\u6587\u4ef6\u548c\u6700\u7ec8\u7ed3\u679c\u653e\u5728\u5bf9\u5e94\u7684 pipestance \u76ee\u5f55\u4e2d\u3002\u6279\u91cf\u4efb\u52a1\u9700\u8981\u63d0\u524d\u7ea6\u5b9a\uff1a<\/p>\n<ul>\n<li>\u6837\u672c\u540d\u4e0e <code>--id<\/code> \u7684\u6620\u5c04\uff1b<\/li>\n<li>FASTQ \u8def\u5f84\u548c\u6587\u5e93\u7c7b\u578b\uff1b<\/li>\n<li>\u53c2\u8003\u5305\u7248\u672c\uff1b<\/li>\n<li>\u6bcf\u4e2a\u4efb\u52a1\u7684\u5de5\u4f5c\u76ee\u5f55\u548c\u5f52\u6863\u76ee\u5f55\uff1b<\/li>\n<li>\u5931\u8d25\u4efb\u52a1\u7684\u65e5\u5fd7\u3001\u91cd\u8dd1\u89c4\u5219\u548c\u4ea4\u4ed8\u6e05\u5355\u3002<\/li>\n<\/ul>\n<p>\u76ee\u5f55\u8fb9\u754c\u6e05\u695a\uff0c\u540e\u7eed\u624d\u80fd\u5224\u65ad\u54ea\u4e2a\u6837\u672c\u5b8c\u6210\u3001\u54ea\u4e2a\u6837\u672c\u9700\u8981\u91cd\u8dd1\uff0c\u4e5f\u80fd\u907f\u514d\u77e9\u9635\u3001BAM \u548c\u8d28\u63a7\u62a5\u544a\u5bf9\u5e94\u9519\u6837\u672c\u3002<\/p>\n<h2>\u54ea\u79cd\u6279\u91cf\u7b56\u7565\u66f4\u5408\u9002\uff1f<\/h2>\n<figure class=\"wp-block-table\">\n<table style=\"display:table;width:100%;min-width:0;table-layout:fixed\">\n<thead>\n<tr>\n<th style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u4efb\u52a1\u60c5\u51b5<\/th>\n<th style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u4e3b\u8981\u98ce\u9669<\/th>\n<th style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u66f4\u5408\u9002\u7684\u5b89\u6392<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">2\u20133 \u4e2a\u89c4\u6a21\u63a5\u8fd1\u7684\u6837\u672c<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u8d44\u6e90\u76f8\u4e92\u4e89\u62a2<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5148\u6d4b\u5355\u4efb\u52a1\uff0c\u518d\u505a\u53d7\u63a7\u5e76\u53d1<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u6837\u672c\u5927\u5c0f\u5dee\u5f02\u660e\u663e<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5927\u4efb\u52a1\u6324\u6389\u5c0f\u4efb\u52a1<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u6309\u6570\u636e\u91cf\u5206\u7ec4\uff0c\u9519\u5cf0\u8fd0\u884c<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u5341\u51e0\u4e2a\u4ee5\u4e0a\u6837\u672c<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u8c03\u5ea6\u3001\u65e5\u5fd7\u548c\u76ee\u5f55\u6df7\u4e71<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u4f7f\u7528\u4efb\u52a1\u961f\u5217\u6216\u96c6\u7fa4\u8c03\u5ea6<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u591a\u4eba\u5171\u4eab\u540c\u4e00\u670d\u52a1\u5668<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u4e92\u76f8\u5360\u6ee1\u5185\u5b58\u548c\u5de5\u4f5c\u76d8<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;padding:10px 8px;vertical-align:top;font-size:15px;line-height:1.65\">\u8bbe\u7f6e\u8d44\u6e90\u4e0a\u9650\u3001\u76ee\u5f55\u548c\u6743\u9650<\/td>\n<\/tr>\n<\/tbody>\n<\/table><figcaption>\u8868\u4e2d\u662f\u8c03\u5ea6\u5224\u65ad\u601d\u8def\u3002\u5b9e\u9645\u5e76\u53d1\u6570\u4ecd\u9700\u6839\u636e\u4ee3\u8868\u6837\u672c\u7684\u5cf0\u503c\u8d44\u6e90\u786e\u5b9a\u3002<\/figcaption><\/figure>\n<h2>\u5927\u6279\u91cf\u4efb\u52a1\uff0c\u4e3a\u4ec0\u4e48\u66f4\u9002\u5408\u670d\u52a1\u5668\u6216\u96c6\u7fa4\uff1f<\/h2>\n<p><a href=\"https:\/\/www.10xgenomics.com\/support\/software\/cell-ranger\/downloads\/cr-system-requirements\" target=\"_blank\" rel=\"noopener noreferrer\">10x Genomics \u5b98\u65b9\u7cfb\u7edf\u8981\u6c42<\/a>\u660e\u786e\u8bf4\u660e\uff0c\u5927\u6570\u636e\u96c6\u9700\u8981\u66f4\u591a\u8d44\u6e90\uff0c\u5e76\u652f\u6301\u5728\u5177\u5907\u5171\u4eab\u6587\u4ef6\u7cfb\u7edf\u548c\u8c03\u5ea6\u7cfb\u7edf\u7684\u96c6\u7fa4\u73af\u5883\u4e2d\u8fd0\u884c\u3002<\/p>\n<p>\u8fd9\u4e0d\u610f\u5473\u7740\u6240\u6709\u6279\u91cf\u4efb\u52a1\u90fd\u5fc5\u987b\u4e0a\u96c6\u7fa4\u3002\u5c11\u91cf\u6837\u672c\u53ef\u4ee5\u5728\u5355\u53f0\u9ad8\u5185\u5b58\u670d\u52a1\u5668\u4e0a\u6309\u961f\u5217\u8fd0\u884c\uff1b\u4efb\u52a1\u6570\u91cf\u589e\u52a0\u540e\uff0c\u518d\u8bc4\u4f30 Slurm \u7b49\u8c03\u5ea6\u65b9\u5f0f\u3002\u5173\u952e\u662f\u8ba9\u8d44\u6e90\u3001\u76ee\u5f55\u3001\u65e5\u5fd7\u548c\u5931\u8d25\u91cd\u8dd1\u6709\u7edf\u4e00\u89c4\u5219\u3002<\/p>\n<h2>\u51c6\u5907\u6279\u91cf\u65b9\u6848\u524d\uff0c\u5148\u6574\u7406\u8fd9\u5f20\u6837\u672c\u6e05\u5355<\/h2>\n<ol>\n<li>\u6837\u672c\u540d\u4e0e\u6587\u5e93\u7c7b\u578b\uff1b<\/li>\n<li>\u6bcf\u4e2a\u6837\u672c FASTQ \u5927\u5c0f\u6216 reads \u6570\uff1b<\/li>\n<li>Cell Ranger \u4e0e\u53c2\u8003\u5305\u7248\u672c\uff1b<\/li>\n<li>\u6bcf\u4e2a\u6837\u672c\u9700\u8981\u4fdd\u7559\u7684\u8f93\u51fa\uff1b<\/li>\n<li>\u662f\u5426\u5141\u8bb8\u5206\u6279\u5b8c\u6210\uff1b<\/li>\n<li>\u5e0c\u671b\u5f00\u59cb\u548c\u5b8c\u6210\u7684\u65f6\u95f4\uff1b<\/li>\n<li>\u56e2\u961f\u4f7f\u7528\u4eba\u6570\u548c\u6570\u636e\u8bbf\u95ee\u8981\u6c42\u3002<\/li>\n<\/ol>\n<h2>\u628a\u6837\u672c\u6e05\u5355\u53d1\u6765\uff0c\u5148\u83b7\u5f97\u6279\u91cf\u8fd0\u884c\u65b9\u6848<\/h2>\n<p>\u5982\u679c\u4f60\u5df2\u7ecf\u6709\u591a\u4e2a 10x \u6837\u672c\uff0c\u4f46\u4e0d\u786e\u5b9a\u670d\u52a1\u5668\u8981\u914d\u591a\u5927\u3001\u4e00\u6b21\u5f00\u51e0\u4e2a\u4efb\u52a1\uff0c\u53ef\u4ee5\u8054\u7cfb\u4e91\u751f\u4fe1\u4f01\u4e1a\u5fae\u4fe1\u5ba2\u670d\uff0c\u53d1\u9001<strong>\u201cCell Ranger 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