{"id":66072,"date":"2026-10-13T15:41:42","date_gmt":"2026-10-13T07:41:42","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/%e5%9f%ba%e5%9b%a0%e5%8d%87%e9%ab%98%e4%ba%86%ef%bc%8c%e5%88%b0%e5%ba%95%e6%98%af%e8%b0%81%e5%9c%a8%e4%bd%bf%e5%8a%b2%ef%bc%9f%e4%b8%80%e5%bc%a0%e5%9b%be%e7%9c%8b%e6%87%82%e5%b8%b8%e8%a7%84%e8%bd%ac\/"},"modified":"2026-10-13T15:42:32","modified_gmt":"2026-10-13T07:42:32","slug":"bulk-vs-scrna","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/bulk-vs-scrna\/","title":{"rendered":"\u57fa\u56e0\u5347\u9ad8\u4e86\uff0c\u5230\u5e95\u662f\u8c01\u5728\u4f7f\u52b2\uff1f\u4e00\u5f20\u56fe\u770b\u61c2\u5e38\u89c4\u8f6c\u5f55\u7ec4\u548c\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u7684\u533a\u522b"},"content":{"rendered":"
\"\u4e00\u5f20\u56fe\u770b\u61c2\u5e38\u89c4\u8f6c\u5f55\u7ec4\u548c\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u7684\u533a\u522b\uff1a\u5e38\u89c4\u8f6c\u5f55\u7ec4\u770b\u4e00\u7fa4\u7ec6\u80de\u7684\u6574\u4f53\u5e73\u5747\u8868\u8fbe\uff0c\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u5206\u7fa4\u770b\u6bcf\u7c7b\u7ec6\u80de\u5206\u522b\u8868\u8fbe\u4ec0\u4e48\"
\u4e00\u5f20\u56fe\u770b\u61c2\uff1a\u5e38\u89c4\u8f6c\u5f55\u7ec4 vs \u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4<\/figcaption><\/figure>\n

\u4f60\u505a\u4e86\u4e00\u6279\u80bf\u7624\u7ec4\u7ec7\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\uff0c\u53d1\u73b0\u67d0\u4e2a\u57fa\u56e0\u663e\u8457\u5347\u9ad8\uff0c\u706b\u5c71\u56fe\u5f88\u6f02\u4eae\uff0c\u901a\u8def\u5bcc\u96c6\u4e5f\u5f88\u6f02\u4eae\u3002\u5199\u5230\u8ba8\u8bba\u90e8\u5206\u65f6\uff0c\u95ee\u9898\u6765\u4e86\uff1a\u8fd9\u4e2a\u57fa\u56e0\u5347\u9ad8\uff0c\u662f\u56e0\u4e3a\u8868\u8fbe\u5b83\u7684\u7ec6\u80de\u53d8\u591a\u4e86\uff0c\u8fd8\u662f\u540c\u6837\u7684\u7ec6\u80de\u6bcf\u4e2a\u90fd\u8868\u8fbe\u5f97\u66f4\u591a\u4e86\uff1f<\/strong><\/p>\n

\u5e38\u89c4\u8f6c\u5f55\u7ec4\u7b54\u4e0d\u4e86\u8fd9\u4e2a\u95ee\u9898\u3002\u8fd9\u4e0d\u662f\u5206\u6790\u6c34\u5e73\u7684\u95ee\u9898\uff0c\u662f\u6280\u672f\u539f\u7406\u51b3\u5b9a\u7684\u3002\u5206\u8fa8\u8fd9\u4e24\u4ef6\u4e8b\uff0c\u9700\u8981\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u3002<\/p>\n

\u5e38\u89c4\u8f6c\u5f55\u7ec4\uff1a\u4e00\u676f”\u6df7\u5408\u679c\u6c41”<\/h2>\n

\u5e38\u89c4\u8f6c\u5f55\u7ec4\uff08bulk RNA-seq\uff09\u628a\u6837\u672c\u91cc\u7684\u5927\u91cf\u7ec6\u80de\u6df7\u5728\u4e00\u8d77\u63d0\u53d6 RNA\uff0c\u7edf\u4e00\u6d4b\u5e8f\u3002\u6253\u4e2a\u6bd4\u65b9\uff1a\u628a\u4e00\u6574\u4e2a\u6c34\u679c\u62fc\u76d8\u5012\u8fdb\u69a8\u6c41\u673a\uff0c\u6253\u51fa\u4e00\u676f\u6df7\u5408\u6c41\u3002\u4f60\u80fd\u5c1d\u51fa”\u8fd9\u676f\u6c41\u6574\u4f53\u6bd4\u53e6\u4e00\u676f\u751c”\uff0c\u4f46\u4f60\u6ca1\u6cd5\u77e5\u9053\u751c\u5473\u4e3b\u8981\u6765\u81ea\u897f\u74dc\u8fd8\u662f\u8292\u679c\uff0c\u66f4\u6ca1\u6cd5\u77e5\u9053\u6bcf\u4e2a\u6c34\u679c\u5404\u81ea\u6709\u591a\u751c\u3002<\/p>\n

\u5b83\u64c5\u957f\u56de\u7b54\uff1a\u4e24\u7ec4\u6837\u672c\u4e4b\u95f4\u54ea\u4e9b\u57fa\u56e0\u6574\u4f53\u8868\u8fbe\u4e0d\u540c\u3001\u5bcc\u96c6\u5728\u54ea\u4e9b\u901a\u8def\u3001\u80fd\u4e0d\u80fd\u627e\u5230\u6f5c\u5728\u6807\u5fd7\u7269\u3002\u5b83\u7684\u76f2\u533a\u662f\u7ec4\u7ec7\u662f\u6df7\u5408\u7269<\/strong>\u2014\u2014\u80bf\u7624\u7ec4\u7ec7\u91cc\u6709\u80bf\u7624\u7ec6\u80de\u3001\u514d\u75ab\u7ec6\u80de\u3001\u6210\u7ea4\u7ef4\u7ec6\u80de\uff0c\u6d4b\u51fa\u6765\u7684\u662f\u6240\u6709\u7ec6\u80de RNA \u7684\u5e73\u5747\u503c\u3002\u5982\u679c\u5904\u7406\u7ec4\u91cc\u514d\u75ab\u7ec6\u80de\u6d78\u6da6\u53d8\u591a\u4e86\uff0cbulk \u6570\u636e\u4e0a\u4f1a\u8868\u73b0\u4e3a\u4e00\u5806\u514d\u75ab\u76f8\u5173\u57fa\u56e0”\u5347\u9ad8”\uff0c\u4f46\u7ec6\u80de\u672c\u8eab\u53ef\u80fd\u4ec0\u4e48\u90fd\u6ca1\u53d8\u3002<\/p>\n

\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\uff1a\u628a\u6c34\u679c\u4e00\u4e2a\u4e2a\u5206\u5f00\u5c1d<\/h2>\n

\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\uff08scRNA-seq\uff09\u628a\u6837\u672c\u6253\u6563\u6210\u5355\u4e2a\u7ec6\u80de\uff0c\u7ed9\u6bcf\u4e2a\u7ec6\u80de\u5355\u72ec\u4e00\u4e2a\u6761\u5f62\u7801\u5206\u5f00\u68c0\u6d4b\u3002\u8fd8\u662f\u90a3\u4e2a\u6c34\u679c\u62fc\u76d8\uff1a\u8fd9\u6b21\u4e0d\u6253\u6c41\u4e86\uff0c\u628a\u6bcf\u4e2a\u6c34\u679c\u5207\u4e0b\u6765\u5355\u72ec\u5c1d\u4e00\u904d\uff0c\u8fd8\u80fd\u987a\u624b\u6309\u79cd\u7c7b\u5206\u5806\uff0c\u518d\u6bd4\u8f83\u8fd9\u76d8\u91cc\u897f\u74dc\u5360\u6bd4\u662f\u4e0d\u662f\u53d8\u591a\u4e86\u3002<\/p>\n

\u5b83\u80fd\u505a\u5230 bulk \u505a\u4e0d\u5230\u7684\u4e09\u4ef6\u4e8b\uff1a<\/p>\n

    \n
  • \u533a\u5206\u7ec6\u80de\u7c7b\u578b\u548c\u4e9a\u7fa4<\/strong>\uff1a\u7ec4\u7ec7\u91cc\u5230\u5e95\u6709\u54ea\u4e9b\u7ec6\u80de\uff0c\u5404\u5360\u591a\u5c11\uff1b<\/li>\n
  • \u5b9a\u4f4d\u53d8\u5316\u6765\u6e90<\/strong>\uff1a\u67d0\u4e2a\u57fa\u56e0\u7684\u53d8\u5316\uff0c\u5177\u4f53\u53d1\u751f\u5728\u54ea\u7c7b\u7ec6\u80de\u8eab\u4e0a\uff1b<\/li>\n
  • \u53d1\u73b0\u7a00\u6709\u7fa4\u4f53<\/strong>\uff1a\u5360\u6bd4 0.5% \u7684\u5c0f\u7ec6\u80de\u7fa4\uff0c\u5728 bulk \u91cc\u4fe1\u53f7\u4f1a\u88ab\u5b8c\u5168\u7a00\u91ca\uff0c\u5355\u7ec6\u80de\u80fd\u76f4\u63a5\u6293\u51fa\u6765\u3002<\/li>\n<\/ul>\n

    \u4e00\u5f20\u8868\u8bf4\u6e05\u4e3b\u8981\u533a\u522b<\/h2>\n
    \n\n\n\n\n\n\n\n\n\n\n
    <\/th>\n\u5e38\u89c4\u8f6c\u5f55\u7ec4<\/th>\n\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4<\/th>\n<\/tr>\n<\/thead>\n
    \u68c0\u6d4b\u5355\u4f4d<\/td>\n\u4e00\u7fa4\u7ec6\u80de\u7684\u6df7\u5408<\/td>\n\u5355\u4e2a\u7ec6\u80de<\/td>\n<\/tr>\n
    \u6838\u5fc3\u8f93\u51fa<\/td>\n\u5dee\u5f02\u57fa\u56e0\u3001\u901a\u8def\u5bcc\u96c6<\/td>\n\u7ec6\u80de\u5206\u7fa4\u3001\u6807\u8bb0\u57fa\u56e0\u3001\u6bd4\u4f8b\u53d8\u5316<\/td>\n<\/tr>\n
    \u80fd\u5426\u5b9a\u4f4d\u7ec6\u80de\u7c7b\u578b<\/td>\n\u4e0d\u80fd\uff0c\u53ea\u6709\u5e73\u5747\u503c<\/td>\n\u80fd\uff0c\u7cbe\u786e\u5230\u4e9a\u7fa4<\/td>\n<\/tr>\n
    \u6bcf\u4e2a\u6837\u672c\u6210\u672c<\/td>\n\u4f4e<\/td>\n\u660e\u663e\u66f4\u9ad8<\/td>\n<\/tr>\n
    \u5206\u6790\u590d\u6742\u5ea6<\/td>\n\u6d41\u7a0b\u6210\u719f\uff0c\u95e8\u69db\u4f4e<\/td>\n\u6570\u636e\u91cf\u5927\uff0c\u9700\u8981\u4e13\u4e1a\u5206\u6790<\/td>\n<\/tr>\n
    \u9002\u5408\u9636\u6bb5<\/td>\n\u5927\u6837\u672c\u9a8c\u8bc1\u3001\u521d\u7b5b<\/td>\n\u673a\u5236\u63a2\u7d22\u3001\u56fe\u8c31\u7ed8\u5236<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

    \u4e25\u683c\u8bf4\uff0c\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u4e5f\u662f\u8f6c\u5f55\u7ec4\u7684\u4e00\u79cd\uff0c\u5e76\u4e0d\u662f\u4e24\u5957\u5b8c\u5168\u4e0d\u540c\u7684\u6280\u672f\uff0c\u66f4\u50cf”\u653e\u5927\u955c\u7248\u672c”\u2014\u2014\u540c\u4e00\u7c7b\u95ee\u9898\uff0c\u4e00\u4e2a\u770b\u5e73\u5747\uff0c\u4e00\u4e2a\u770b\u7ec6\u8282\u3002\u5f88\u591a\u9ad8\u5206\u6587\u7ae0\u7684\u505a\u6cd5\u662f\uff1a\u5355\u7ec6\u80de\u8d1f\u8d23\u53d1\u73b0\uff08\u54ea\u4e9b\u7ec6\u80de\u3001\u54ea\u4e9b\u4e9a\u7fa4\uff09\uff0cbulk \u8d1f\u8d23\u9a8c\u8bc1\uff08\u5927\u6837\u672c\u786e\u8ba4\u7ed3\u8bba\u7ad9\u5f97\u4f4f\uff09<\/strong>\uff0c\u4e24\u8005\u642d\u914d\u6bd4\u5355\u6253\u72ec\u6597\u8bf4\u670d\u529b\u5f3a\u5f97\u591a\u3002<\/p>\n

    \u600e\u4e48\u9009\uff1a\u4e09\u4e2a\u95ee\u9898\u95ee\u81ea\u5df1<\/h2>\n

    1. \u4f60\u7684\u79d1\u5b66\u95ee\u9898\u9700\u8981\u5b9a\u4f4d\u5230\u7ec6\u80de\u5417\uff1f<\/strong>\u53ea\u95ee”\u4e24\u7ec4\u6574\u4f53\u6709\u4ec0\u4e48\u4e0d\u540c”\u2014\u2014bulk \u591f\u4e86\uff0c\u6027\u4ef7\u6bd4\u9ad8\u3002\u8981\u95ee”\u53d8\u5316\u53d1\u751f\u5728\u54ea\u7c7b\u7ec6\u80de\u3001\u7ec6\u80de\u7ec4\u6210\u6709\u6ca1\u6709\u53d8”\u2014\u2014\u5fc5\u987b\u5355\u7ec6\u80de\u3002<\/p>\n

    2. \u6837\u672c\u91cf\u548c\u9884\u7b97\u591a\u5c11\uff1f<\/strong>bulk \u4e00\u4e2a\u6837\u672c\u51e0\u767e\u5230\u4e00\u5343\u591a\uff0c\u5355\u7ec6\u80de\u4e00\u4e2a\u6837\u672c\u51e0\u5343\u8d77\u6b65\u3002\u5927\u6837\u672c\u961f\u5217\u5168\u505a\u5355\u7ec6\u80de\uff0c\u591a\u6570\u8bfe\u9898\u7ec4\u5403\u4e0d\u6d88\uff1b\u5148\u5c0f\u89c4\u6a21\u5355\u7ec6\u80de\u63a2\u8def\u3001\u518d\u5927\u6837\u672c bulk \u9a8c\u8bc1\uff0c\u662f\u66f4\u5e38\u89c1\u7684\u6253\u6cd5\u3002<\/p>\n

    3. \u8c01\u6765\u505a\u5206\u6790\uff1f<\/strong>\u5355\u7ec6\u80de\u7684\u6570\u636e\u91cf\u52a8\u8f84\u51e0\u4e2a GB \u5230\u51e0\u5341 GB\uff0c\u5206\u6790\u73af\u5883\u5403\u5185\u5b58\u5403\u7b97\u529b\uff0cSeurat\/Scanpy \u6d41\u7a0b\u5bf9\u65b0\u624b\u5e76\u4e0d\u53cb\u597d\u3002\u670d\u52a1\u5668\u914d\u7f6e\u3001\u5206\u6790\u4eba\u529b\uff0c\u8fd9\u4e24\u5757\u6210\u672c\u5728\u8bbe\u8ba1\u8bfe\u9898\u65f6\u5c31\u8981\u7b97\u8fdb\u53bb\uff0c\u522b\u7b49\u6570\u636e\u4e0b\u673a\u4e86\u624d\u53d1\u73b0\u8dd1\u4e0d\u52a8\u3002<\/p>\n

    \u548c\u4e91\u751f\u4fe1\u5408\u4f5c\uff0c\u9009\u9002\u5408\u4f60\u7684\u65b9\u5f0f<\/h2>\n

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