{"id":66076,"date":"2026-10-14T20:00:00","date_gmt":"2026-10-14T12:00:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66076"},"modified":"2026-10-13T16:25:15","modified_gmt":"2026-10-13T08:25:15","slug":"dual-virtual-knockout-screening-guide","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/dual-virtual-knockout-screening-guide\/","title":{"rendered":"\u53cc\u865a\u62df\u600e\u4e48\u505a\u624d\u6709\u4ef7\u503c\uff1f\u5148\u628a\u201c\u5019\u9009\u57fa\u56e0\u201d\u63a5\u5230\u53ef\u9a8c\u8bc1\u7684\u95ee\u9898\u4e0a"},"content":{"rendered":"<p>\u5355\u7ec6\u80de\u5206\u7fa4\u505a\u5b8c\u4e86\uff0c\u5dee\u5f02\u57fa\u56e0\u5217\u51fa\u6765\u4e86\uff0c\u5bcc\u96c6\u56fe\u4e5f\u9f50\u4e86\u3002\u4e0b\u4e00\u6b65\u9009\u54ea\u4e2a\u57fa\u56e0\u505a\u5b9e\u9a8c\uff0c\u53cd\u800c\u66f4\u96be\u3002<\/p>\n<p>\u770b\u5230\u522b\u4eba\u628a\u201c\u865a\u62df\u6572\u9664\u201d\u201c\u865a\u62df\u7b5b\u9009\u201d\u63a5\u8fdb\u6587\u7ae0\uff0c\u4f60\u53ef\u80fd\u4e5f\u60f3\u52a0\u4e0a\u8fd9\u4e24\u6b65\u3002\u4f46\u5728\u771f\u6b63\u52a8\u624b\u4e4b\u524d\uff0c\u503c\u5f97\u5148\u95ee\uff1a<strong>\u5b83\u4eec\u80fd\u5e2e\u4f60\u6dd8\u6c70\u54ea\u4e9b\u5019\u9009\uff0c\u53c8\u80fd\u4e3a\u540e\u7eed\u5b9e\u9a8c\u589e\u52a0\u4ec0\u4e48\u4f9d\u636e\uff1f<\/strong><\/p>\n<h2>01\uff5c\u5148\u628a\u201c\u53cc\u865a\u62df\u201d\u62c6\u6210\u4e24\u4e2a\u95ee\u9898<\/h2>\n<p>\u672c\u6587\u8ba8\u8bba\u7684\u662f\u201c\u865a\u62df\u6572\u9664\uff0b\u865a\u62df\u7b5b\u9009\u201d\u8fd9\u7c7b\u7ec4\u5408\u3002\u201c\u53cc\u865a\u62df\u201d\u53ea\u662f\u6c9f\u901a\u7b80\u79f0\uff0c\u5177\u4f53\u4f7f\u7528\u54ea\u4e9b\u65b9\u6cd5\u3001\u9884\u6d4b\u4ec0\u4e48\u5bf9\u8c61\uff0c\u4ecd\u8981\u5199\u6e05\u695a\u3002<\/p>\n<p>\u865a\u62df\u6572\u9664\u95ee\u7684\u662f\uff1a\u5728\u4e00\u4e2a\u8ba1\u7b97\u6a21\u578b\u91cc\u524a\u5f31\u6216\u79fb\u9664\u76ee\u6807\u57fa\u56e0\u7684\u4f5c\u7528\uff0c\u54ea\u4e9b\u57fa\u56e0\u6216\u7ec6\u80de\u72b6\u6001\u53ef\u80fd\u968f\u4e4b\u53d8\u5316\uff1f\u4f8b\u5982 scTenifoldKnk \u4ece\u5355\u7ec6\u80de\u8868\u8fbe\u6570\u636e\u6784\u5efa\u8c03\u63a7\u7f51\u7edc\uff0c\u518d\u6bd4\u8f83\u8ba1\u7b97\u6270\u52a8\u524d\u540e\u7684\u7f51\u7edc\u53d8\u5316\uff0c\u8f93\u51fa\u53d7\u5f71\u54cd\u57fa\u56e0\u7684\u4f18\u5148\u7ea7\u3002\u5b83\u7ed9\u51fa\u7684\u662f\u6a21\u578b\u9884\u6d4b\uff0c\u4e0d\u662f\u5df2\u7ecf\u5728\u771f\u5b9e\u7ec6\u80de\u91cc\u5b8c\u6210\u6572\u9664\u7684\u8bc1\u636e\u3002<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35510185\/\">[1]<\/a><\/p>\n<p>\u865a\u62df\u7b5b\u9009\u95ee\u7684\u662f\uff1a\u54ea\u4e9b\u5019\u9009\u5206\u5b50\u503c\u5f97\u8fdb\u4e00\u6b65\u7814\u7a76\uff1f\u8fd9\u91cc\u8fd8\u8981\u533a\u5206\u4e24\u6761\u8def\u7ebf\uff1a\u7528\u8868\u8fbe\u7b7e\u540d\u5bfb\u627e\u53ef\u80fd\u9006\u8f6c\u67d0\u79cd\u72b6\u6001\u7684\u5019\u9009\uff0c\u6216\u8005\u6839\u636e\u9776\u70b9\u7ed3\u6784\u8fdb\u884c\u5206\u5b50\u5bf9\u63a5\u3002\u4e24\u8005\u8f93\u5165\u3001\u8bc4\u5206\u548c\u7ed3\u8bba\u4e0d\u540c\uff0c\u4e0d\u80fd\u53ea\u7528\u4e00\u5f20\u201c\u836f\u7269\u9884\u6d4b\u56fe\u201d\u6df7\u5728\u4e00\u8d77\u3002<\/p>\n<h2>02\uff5c\u4e24\u4e2a\u6a21\u5757\u4e4b\u95f4\uff0c\u5fc5\u987b\u6709\u4e00\u6761\u89e3\u91ca\u5f97\u901a\u7684\u8fde\u63a5<\/h2>\n<p>\u5047\u8bbe\u4f60\u7814\u7a76\u67d0\u79cd\u75be\u75c5\u4e2d\u7684\u9ad3\u7cfb\u7ec6\u80de\uff0c\u7b5b\u5230\u5019\u9009\u57fa\u56e0 A\u3002\u8fd9\u662f\u4e00\u4e2a<strong>\u6559\u5b66\u573a\u666f\uff0c\u4e0d\u662f\u771f\u5b9e\u5ba2\u6237\u6848\u4f8b<\/strong>\u3002<\/p>\n<p>\u5408\u7406\u7684\u4e0b\u4e00\u6b65\u662f\u5148\u786e\u8ba4 A \u5728\u76ee\u6807\u4e9a\u7fa4\u4e2d\u7684\u8868\u8fbe\u3001\u6837\u672c\u8986\u76d6\u548c\u7f51\u7edc\u4f4d\u7f6e\uff1b\u518d\u89c2\u5bdf\u6a21\u578b\u6270\u52a8\u540e\uff0c\u662f\u5426\u51fa\u73b0\u4e0e\u7814\u7a76\u95ee\u9898\u6709\u5173\u7684\u901a\u8def\u6216\u72b6\u6001\u53d8\u5316\u3002\u5982\u679c\u8981\u5bfb\u627e\u5e72\u9884\u5019\u9009\uff0c\u8fd8\u9700\u8bf4\u660e\u5e0c\u671b\u6291\u5236\u3001\u589e\u5f3a\u8fd8\u662f\u9006\u8f6c\u4ec0\u4e48\u72b6\u6001\u3002<\/p>\n<p>\u5982\u679c A \u7684\u865a\u62df\u6572\u9664\u7ed3\u679c\u63d0\u793a\u67d0\u6761\u901a\u8def\u6539\u53d8\uff0c\u968f\u540e\u5374\u5bf9\u4e00\u4e2a\u7f3a\u5c11\u5173\u8054\u89e3\u91ca\u7684\u86cb\u767d\u8fdb\u884c\u5bf9\u63a5\uff0c\u8fd9\u4e24\u90e8\u5206\u5373\u4f7f\u5206\u522b\u5f97\u5230\u6f02\u4eae\u56fe\u7247\uff0c\u4e5f\u6ca1\u6709\u56de\u7b54\u540c\u4e00\u4e2a\u95ee\u9898\u3002<\/p>\n<figure class=\"wp-block-table\">\n<table class=\"virtual-comparison\">\n<tr>\n<th>\u5f53\u524d\u6761\u4ef6<\/th>\n<th>\u5148\u68c0\u67e5\u4ec0\u4e48<\/th>\n<th>\u4e0b\u4e00\u6b65<\/th>\n<\/tr>\n<tr>\n<td>\u53ea\u6709\u5019\u9009\u57fa\u56e0\u540d\u5355<\/td>\n<td>\u76ee\u6807\u7ec6\u80de\u4e0e\u6837\u672c\u8986\u76d6<\/td>\n<td>\u5148\u5b9a\u4f4d\u7ec6\u80de\u573a\u666f<\/td>\n<\/tr>\n<tr>\n<td>\u6709\u76ee\u6807\u4e9a\u7fa4\u8868\u8fbe\u6570\u636e<\/td>\n<td>\u65b9\u6cd5\u8f93\u5165\u4e0e\u7f51\u7edc\u7a33\u5b9a\u6027<\/td>\n<td>\u505a\u5c0f\u8303\u56f4\u6270\u52a8\u9884\u6d4b<\/td>\n<\/tr>\n<tr>\n<td>\u8981\u7b5b\u5019\u9009\u5206\u5b50<\/td>\n<td>\u8868\u8fbe\u7b7e\u540d\u6216\u7ed3\u6784\u4f9d\u636e<\/td>\n<td>\u9009\u5b9a\u7b5b\u9009\u8def\u7ebf<\/td>\n<\/tr>\n<tr>\n<td>\u9884\u6d4b\u7ed3\u679c\u65b9\u5411\u4e0d\u4e00\u81f4<\/td>\n<td>\u6570\u636e\u3001\u6a21\u578b\u548c\u53c2\u6570\u5dee\u5f02<\/td>\n<td>\u4fdd\u7559\u51b2\u7a81\uff0c\u8865\u9a8c\u8bc1<\/td>\n<\/tr>\n<\/table>\n<\/figure>\n<p>\u8fd9\u5f20\u8868\u662f\u4e00\u4efd\u884c\u52a8\u5224\u65ad\u8868\uff0c\u4e0d\u662f\u201c\u6ee1\u8db3\u6761\u4ef6\u5c31\u4e00\u5b9a\u6210\u529f\u201d\u7684\u8bc4\u5206\u8868\u3002\u6700\u503c\u5f97\u4f18\u5148\u89e3\u51b3\u7684\uff0c\u901a\u5e38\u662f\u4e24\u4e2a\u6a21\u5757\u4e4b\u95f4\u7f3a\u5931\u7684\u90a3\u4e00\u6b65\u3002<\/p>\n<figure class=\"wp-block-image size-full\" style=\"margin:28px 0\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/virtual-series-20261012-01-poster.png?resize=1080%2C1440&#038;ssl=1\" width=\"1080\" height=\"1440\" loading=\"lazy\" alt=\"\u5019\u9009\u57fa\u56e0\u627e\u5230\u4e86\uff0c\u4e0b\u4e00\u6b65\u600e\u4e48\u9a8c\u8bc1\uff1f\uff1b\u865a\u62df\u6572\u9664 \u00d7 \u865a\u62df\u7b5b\u9009\uff1b\u626b\u7801\u54a8\u8be2\u7814\u7a76\u8def\u7ebf\u8bc4\u4f30\"><\/figure>\n<p>\u6d77\u62a5\u91cc\u7684\u4e09\u6b65\u5bf9\u5e94\u540c\u4e00\u4e2a\u76ee\u6807\uff1a\u628a\u5019\u9009\u7f29\u5c0f\u5230\u53ef\u4ee5\u7ee7\u7eed\u9a8c\u8bc1\u7684\u8303\u56f4\uff0c\u5e76\u63d0\u524d\u770b\u6e05\u9700\u8981\u8865\u4ec0\u4e48\u8d44\u6599\u3002<\/p>\n<h2>03\uff5c\u4e0d\u8981\u628a\u8ba1\u7b97\u9884\u6d4b\u5199\u6210\u5b9e\u9a8c\u9a8c\u8bc1<\/h2>\n<p>\u865a\u62df\u6572\u9664\u7ed3\u679c\u53ef\u4ee5\u652f\u6301\u201c\u8be5\u57fa\u56e0\u503c\u5f97\u8fdb\u4e00\u6b65\u7814\u7a76\u201d\u7684\u5224\u65ad\uff1b\u5206\u5b50\u5bf9\u63a5\u53ef\u4ee5\u63d0\u4f9b\u7ed3\u5408\u6784\u8c61\u548c\u8bc4\u5206\u7ebf\u7d22\u3002\u5bf9\u63a5\u8bc4\u5206\u672c\u8eab\u4e0d\u80fd\u8bc1\u660e\u5316\u5408\u7269\u5177\u6709\u7ec6\u80de\u5185\u836f\u6548\uff0c\u4e5f\u4e0d\u80fd\u76f4\u63a5\u5224\u5b9a\u5b83\u662f\u6fc0\u52a8\u5242\u8fd8\u662f\u6291\u5236\u5242\u3002Vina \u5b98\u65b9\u8bf4\u660e\u540c\u6837\u5efa\u8bae\u9488\u5bf9\u5177\u4f53\u9776\u70b9\u7528\u5df2\u77e5\u6d3b\u6027\u5206\u5b50\u6216\u7ed3\u5408\u7ed3\u6784\u8bc4\u4f30\u65b9\u6cd5\u8868\u73b0\u3002<a href=\"https:\/\/vina.scripps.edu\/manual\/\">[2]<\/a><\/p>\n<p>\u53ef\u4ee5\u63d0\u524d\u5199\u51fa\u4e00\u5f20\u5b9e\u9a8c\u8854\u63a5\u6e05\u5355\uff1a\u9700\u8981\u89c2\u5bdf\u4ec0\u4e48\u8868\u578b\u3001\u7528\u54ea\u4e9b\u5bf9\u7167\u3001\u600e\u6837\u786e\u8ba4\u6270\u52a8\u662f\u5426\u6210\u529f\uff0c\u4ee5\u53ca\u4ec0\u4e48\u7ed3\u679c\u4f1a\u8ba9\u4f60\u653e\u5f03\u5f53\u524d\u5047\u8bbe\u3002\u8fd9\u6837\u5f97\u5230\u7684\u9884\u6d4b\u66f4\u5bb9\u6613\u8f6c\u5316\u4e3a\u884c\u52a8\u3002<\/p>\n<h2>04\uff5c\u5148\u8dd1\u5c0f\u8303\u56f4\uff0c\u518d\u51b3\u5b9a\u6295\u5165\u591a\u5c11\u7b97\u529b<\/h2>\n<p>\u53ea\u6709\u5c11\u91cf\u5019\u9009\u3001\u76ee\u6807\u4e9a\u7fa4\u4e5f\u8f83\u6e05\u695a\u65f6\uff0c\u53ef\u4ee5\u5148\u590d\u73b0\u5b98\u65b9\u793a\u4f8b\uff0c\u518d\u7528\u7ea6\u5b9a\u5b50\u96c6\u8bd5\u8dd1\u3002\u591a\u57fa\u56e0\u3001\u591a\u4e9a\u7fa4\u3001\u591a\u4e2a\u53c2\u6570\u91cd\u590d\u540e\uff0c\u624d\u9700\u8981\u7ed3\u5408\u5cf0\u503c\u5185\u5b58\u548c\u8fd0\u884c\u65f6\u95f4\u8003\u8651\u6269\u5bb9\u3002\u591a\u79df\u51e0\u53f0\u670d\u52a1\u5668\u4e0d\u80fd\u4fee\u590d\u4e0d\u5408\u9002\u7684\u7814\u7a76\u8bbe\u8ba1\u3002<\/p>\n<p>\u5982\u679c\u5361\u70b9\u662f\u201c\u8be5\u9009\u54ea\u6761\u8def\u7ebf\u201d\uff0c\u4e91\u751f\u4fe1\u63d0\u4f9b\u7814\u7a76\u8bfe\u9898\u4e0e\u5206\u6790\u8def\u7ebf\u8bc4\u4f30\uff1b\u5982\u679c\u8def\u7ebf\u5df2\u660e\u786e\uff0c\u5361\u5728\u73af\u5883\u6216\u8fd0\u884c\u89c4\u6a21\uff0c\u518d\u8ba8\u8bba\u670d\u52a1\u5668\u4e0e\u6267\u884c\u65b9\u5f0f\u3002\u8bc4\u4f30\u7684\u8f93\u5165\u3001\u4ea4\u4ed8\u3001\u5468\u671f\u548c\u8d39\u7528\u5728\u5f00\u59cb\u524d\u786e\u8ba4\u3002<\/p>\n<div class=\"cta\">\n<h2>\u5df2\u7ecf\u6709\u6570\u636e\uff0c\u5374\u4e0d\u77e5\u9053\u600e\u4e48\u628a\u53cc\u865a\u62df\u63a5\u8d77\u6765\uff1f<\/h2>\n<p>\u8054\u7cfb\u4e91\u751f\u4fe1\uff0c\u53d1\u9001\u201c<strong>\u53cc\u865a\u62df<\/strong>\uff0b\u7814\u7a76\u76ee\u6807\uff0b\u6570\u636e\u7c7b\u578b\u4e0e\u89c4\u6a21\uff0b\u5019\u9009\u57fa\u56e0\/\u5f53\u524d\u5361\u70b9\u201d\u3002\u5ba2\u670d\u5148\u6838\u5bf9\u8d44\u6599\u4e0e\u9002\u914d\u8303\u56f4\uff0c\u518d\u5bf9\u63a5\u8def\u7ebf\u8bc4\u4f30\uff0c\u660e\u786e\u53ef\u6267\u884c\u6b65\u9aa4\u548c\u9700\u8981\u8865\u9f50\u7684\u8bc1\u636e\u3002<\/p>\n<p><a class=\"button\" target=\"_blank\" rel=\"noopener noreferrer\" data-ysx-event=\"contact_click\" data-ysx-source=\"virtual-series-20261012-01\" href=\"https:\/\/work.weixin.qq.com\/kfid\/kfcb05492f29d4d6064\">\u54a8\u8be2\u7814\u7a76\u8def\u7ebf\u8bc4\u4f30 \u2192<\/a><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u865a\u62df\u6572\u9664\u548c\u865a\u62df\u7b5b\u9009\u5206\u522b\u56de\u7b54\u4ec0\u4e48\u95ee\u9898\uff1f\u4ece\u76ee\u6807\u7ec6\u80de\u3001\u5019\u9009\u57fa\u56e0\u5230\u5e72\u9884\u65b9\u5411\uff0c\u672c\u6587\u7528\u5224\u65ad\u8868\u8bf4\u660e\u4e24\u7c7b\u65b9\u6cd5\u5982\u4f55\u8854\u63a5\uff0c\u4ee5\u53ca\u8ba1\u7b97\u9884\u6d4b\u8fd8\u9700\u8981\u54ea\u4e9b\u9a8c\u8bc1\u3002\u5df2\u6709\u6570\u636e\u5374\u62ff\u4e0d\u51c6\u8def\u7ebf\u7684\u56e2\u961f\uff0c\u53ef\u54a8\u8be2\u7814\u7a76\u8bfe\u9898\u4e0e\u5206\u6790\u8def\u7ebf\u8bc4\u4f30\u3002<\/p>\n","protected":false},"author":1,"featured_media":66074,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[1,71],"tags":[90,335,336,333,334],"class_list":["post-66076","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-part","category-single-cell","tag-90","tag-335","tag-336","tag-333","tag-334"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/virtual-series-20261012-01-cover.png?fit=1200%2C510&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66076","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=66076"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66076\/revisions"}],"predecessor-version":[{"id":66086,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66076\/revisions\/66086"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/66074"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=66076"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=66076"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=66076"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}