{"id":66218,"date":"2026-10-17T09:07:59","date_gmt":"2026-10-17T01:07:59","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66218"},"modified":"2026-10-17T09:07:59","modified_gmt":"2026-10-17T01:07:59","slug":"cell-ranger-arc-2-2-multiome-resource-planning","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/cell-ranger-arc-2-2-multiome-resource-planning\/","title":{"rendered":"\u5355\u7ec6\u80de Multiome \u4e3a\u4ec0\u4e48 64 GB \u80fd\u542f\u52a8\u3001\u5374\u8dd1\u4e0d\u7a33\uff1fCell Ranger ARC 2.2 \u8d44\u6e90\u89c4\u5212"},"content":{"rendered":"
\n

\u505a 10x Multiome ATAC + Gene Expression \u65f6\uff0c\u6700\u5bb9\u6613\u8e29\u7684\u5751\u4e0d\u662f\u547d\u4ee4\u4e0d\u4f1a\u5199\uff0c\u800c\u662f\u628a\u201c\u6700\u4f4e\u914d\u7f6e\u201d\u5f53\u6210\u201c\u7a33\u5b9a\u914d\u7f6e\u201d\uff1a\u4efb\u52a1\u80fd\u542f\u52a8\uff0c\u8dd1\u5230\u4e2d\u6bb5\u5374\u56e0\u5185\u5b58\u3001\u78c1\u76d8\u6216\u5e76\u53d1\u88ab\u6740\uff1b\u6362\u66f4\u5927\u673a\u5668\u540e\uff0c\u53c8\u53d1\u73b0\u6838\u6570\u7ffb\u500d\u5e76\u6ca1\u6709\u7b49\u6bd4\u4f8b\u63d0\u901f\u3002<\/p>\n

2026 \u5e74 4 \u6708\u53d1\u5e03\u7684 Cell Ranger ARC 2.2 \u5df2\u652f\u6301 GEM-X Epi Multiome\uff0c\u5e76\u80fd\u5728\u672c\u5730\u8fd0\u884c Pan-Human Azimuth \u7ec6\u80de\u6ce8\u91ca\u3002\u529f\u80fd\u66f4\u5b8c\u6574\uff0c\u4e5f\u610f\u5473\u7740\u5206\u6790\u524d\u66f4\u5e94\u628a\u7248\u672c\u3001\u53c2\u8003\u6570\u636e\u3001\u8d44\u6e90\u4e0a\u9650\u548c\u4ea4\u4ed8\u8303\u56f4\u4e00\u8d77\u89c4\u5212\uff0c\u800c\u4e0d\u662f\u7b49\u62a5\u9519\u540e\u518d\u4e34\u65f6\u52a0\u673a\u5668\u3002<\/p>\n

\"Cell
Cell Ranger ARC 2.2 \u8d44\u6e90\u89c4\u5212\uff1a\u6700\u4f4e\u914d\u7f6e\u53ea\u662f\u8d77\u70b9\uff0c\u5e76\u53d1\u4e0e\u78c1\u76d8\u540c\u6837\u8981\u63d0\u524d\u8ba1\u7b97\u3002<\/figcaption><\/figure>\n

\u5148\u770b\u7ed3\u8bba\uff1a64 GB \u662f\u95e8\u69db\uff0c\u4e0d\u662f\u6240\u6709\u9879\u76ee\u7684\u8212\u9002\u533a<\/h2>\n

10x Genomics \u5f53\u524d\u7ed9\u51fa\u7684 ARC 2.2 \u5355\u673a\u6700\u4f4e\u8981\u6c42\u662f 8 \u6838\u300164 GB \u5185\u5b58\u30011 TB \u53ef\u7528\u78c1\u76d8<\/strong>\uff0c\u63a8\u8350\u503c\u4e3a 48 \u6838\u3001320 GB \u5185\u5b58<\/strong>\u3002\u5b98\u65b9\u8fd8\u7528 PBMC 10k \u6570\u636e\u505a\u8fc7\u6d4b\u8bd5\uff1a8 \u7ebf\u7a0b\u300164 GB \u7684\u5899\u949f\u65f6\u95f4\u7ea6 14 \u5c0f\u65f6 08 \u5206<\/strong>\uff1b32 \u7ebf\u7a0b\u3001256 GB \u7ea6 4 \u5c0f\u65f6 50 \u5206<\/strong>\uff1b48 \u7ebf\u7a0b\u3001384 GB \u7ea6 2 \u5c0f\u65f6 59 \u5206<\/strong>\u3002\u8fd9\u4e9b\u6570\u5b57\u80fd\u5e2e\u52a9\u4f30\u7b97\u91cf\u7ea7\uff0c\u4f46\u4e0d\u80fd\u76f4\u63a5\u5f53\u6210\u81ea\u5df1\u6837\u672c\u7684\u627f\u8bfa\u2014\u2014\u7ec6\u80de\u6570\u3001\u6bcf\u7ec6\u80de reads\u3001\u78c1\u76d8\u6027\u80fd\u548c\u5e76\u53d1\u90fd\u4f1a\u6539\u53d8\u7ed3\u679c\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n\n
\u8d44\u6e90\u6216\u8bbe\u7f6e<\/th>\n\u5b98\u65b9\u4fe1\u606f<\/th>\n\u9879\u76ee\u4e0a\u8be5\u600e\u4e48\u7406\u89e3<\/th>\n<\/tr>\n<\/thead>\n
\u6700\u4f4e\u786c\u4ef6<\/td>\n8 \u6838 \/ 64 GB \/ 1 TB<\/td>\n\u80fd\u8fdb\u5165\u5206\u6790\u6d41\u7a0b\uff0c\u4e0d\u4ee3\u8868\u9002\u5408\u591a\u4e2a\u6837\u672c\u5e76\u884c<\/td>\n<\/tr>\n
\u63a8\u8350\u786c\u4ef6<\/td>\n48 \u6838 \/ 320 GB<\/td>\n\u66f4\u63a5\u8fd1\u7a33\u5b9a\u4ea4\u4ed8\u914d\u7f6e\uff0c\u4ecd\u9700\u6309\u6570\u636e\u91cf\u5b9e\u6d4b<\/td>\n<\/tr>\n
PBMC 10k \u5b9e\u6d4b<\/td>\n64 GB\uff1a14h08m\uff1b256 GB\uff1a4h50m<\/td>\n\u52a0\u5185\u5b58\u548c\u7ebf\u7a0b\u53ef\u660e\u663e\u63d0\u901f\uff0c\u4f46\u4e0d\u662f\u7ebf\u6027\u6536\u76ca<\/td>\n<\/tr>\n
\u672c\u5730\u6a21\u5f0f\u9ed8\u8ba4\u503c<\/td>\n\u5168\u90e8\u53ef\u7528\u6838\u5fc3 + 90% \u5185\u5b58<\/td>\n\u5171\u4eab\u670d\u52a1\u5668\u4e0a\u82e5\u4e0d\u8bbe\u4e0a\u9650\uff0c\u5bb9\u6613\u6324\u538b\u5176\u4ed6\u4efb\u52a1<\/td>\n<\/tr>\n
64 GB \u8c03\u5ea6\u793a\u4f8b<\/td>\n--localmem=57<\/code><\/td>\n\u7ed9\u7cfb\u7edf\u548c\u8c03\u5ea6\u5668\u7559\u4f59\u91cf\uff0c\u4e0d\u8981\u628a\u7269\u7406\u5185\u5b58\u5168\u90e8\u62a5\u7ed9\u6d41\u7a0b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u4e3a\u4ec0\u4e48 Multiome \u6bd4\u666e\u901a\u8868\u8fbe\u77e9\u9635\u66f4\u5403\u8d44\u6e90\uff1f<\/h2>\n

Multiome \u4e0d\u662f\u628a\u4e24\u4e2a\u7ed3\u679c\u6587\u4ef6\u7b80\u5355\u62fc\u5728\u4e00\u8d77\u3002\u540c\u4e00\u6279\u7ec6\u80de\u9700\u8981\u540c\u65f6\u5904\u7406 ATAC \u4e0e GEX \u4e24\u7c7b FASTQ\uff0c\u5b8c\u6210\u6bd4\u5bf9\u3001\u6761\u5f62\u7801\u517c\u5bb9\u6027\u68c0\u67e5\u3001\u7247\u6bb5\u4e0e\u5cf0\u76f8\u5173\u8ba1\u7b97\u3001\u8868\u8fbe\u77e9\u9635\u751f\u6210\u4ee5\u53ca\u8054\u5408\u964d\u7ef4\u548c\u805a\u7c7b\u3002\u9ad8\u5cf0\u5185\u5b58\u53ef\u80fd\u96c6\u4e2d\u5728\u67d0\u4e2a\u9636\u6bb5\uff0c\u78c1\u76d8\u8fd8\u8981\u540c\u65f6\u5bb9\u7eb3\u8f93\u5165\u3001\u53c2\u8003\u5305\u3001\u4e34\u65f6\u6587\u4ef6\u548c\u6700\u7ec8\u8f93\u51fa\u3002\u53ea\u770b FASTQ \u603b\u5927\u5c0f\uff0c\u5f80\u5f80\u4f1a\u4f4e\u4f30\u5cf0\u503c\u8d44\u6e90\u3002<\/p>\n

\u6b64\u5916\uff0cARC \u672c\u5730\u6a21\u5f0f\u9ed8\u8ba4\u4f1a\u4f7f\u7528\u68c0\u6d4b\u5230\u7684\u5168\u90e8\u6838\u5fc3\u548c 90% \u5185\u5b58\u3002\u4e00\u53f0 128 GB \u5171\u4eab\u8282\u70b9\u4e0a\uff0c\u5982\u679c\u4e24\u4e2a\u7528\u6237\u5404\u542f\u52a8\u4e00\u4e2a\u4efb\u52a1\uff0c\u4e24\u4e2a\u6d41\u7a0b\u90fd\u8ba4\u4e3a\u81ea\u5df1\u80fd\u62ff\u5230\u7ea6 115 GB\uff0c\u5b9e\u9645\u53ef\u7528\u91cf\u5374\u4e0d\u4f1a\u51ed\u7a7a\u7ffb\u500d\u3002\u8fd9\u7c7b\u201c\u5076\u53d1 OOM\u201d\u5e38\u88ab\u8bef\u5224\u4e3a\u8f6f\u4ef6\u4e0d\u7a33\u5b9a\uff0c\u6839\u56e0\u5176\u5b9e\u662f\u8d44\u6e90\u8fb9\u754c\u6ca1\u6709\u5199\u8fdb\u547d\u4ee4\u548c\u8c03\u5ea6\u7b56\u7565\u3002<\/p>\n

\u4e00\u6761\u547d\u4ee4\uff0c\u81f3\u5c11\u628a\u56db\u4ef6\u4e8b\u8bf4\u6e05\u695a<\/h2>\n
cellranger-arc count \\\r\n  --id=sample01 \\\r\n  --reference=\/data\/refdata-cellranger-arc-GRCh38-2024-A \\\r\n  --libraries=libraries.csv \\\r\n  --localcores=32 \\\r\n  --localmem=240 \\\r\n  --create-bam=true\r\n<\/code><\/pre>\n
\u8fd9\u91cc\u7684 32 \u6838\u548c 240 GB \u53ea\u662f\u793a\u4f8b\uff0c\u4e0d\u662f\u901a\u7528\u7b54\u6848\u3002\u771f\u6b63\u8981\u786e\u8ba4\u7684\u662f\uff1a\u8f6f\u4ef6\u7248\u672c\u4e0e\u53c2\u8003\u5305\u662f\u5426\u5339\u914d\u3001\u8282\u70b9\u662f\u5426\u652f\u6301 AVX\u3001ATAC\/GEX \u8def\u5f84\u548c library type \u662f\u5426\u6b63\u786e\u3001\u540e\u7eed\u662f\u5426\u9700\u8981 BAM\u3002ARC 2.2 \u7684 --create-bam<\/code> \u5fc5\u987b\u660e\u786e\u7ed9\u51fa true \u6216 false\uff1b\u82e5\u540e\u7eed\u4e0d\u505a\u6d4f\u89c8\u5668\u6838\u67e5\u6216\u91cd\u6bd4\u5bf9\uff0c\u5173\u95ed BAM \u80fd\u51cf\u5c11\u5b58\u50a8\u538b\u529b\uff0c\u4f46\u8fd9\u662f\u5206\u6790\u65b9\u6848\u9009\u62e9\uff0c\u4e0d\u80fd\u4e3a\u4e86\u7701\u76d8\u76f2\u76ee\u5173\u95ed\u3002<\/p>\n
\u5982\u679c\u53ea\u60f3\u5148\u9a8c\u8bc1\u4e3b\u6d41\u7a0b\uff0c--nosecondary<\/code> \u53ef\u8df3\u8fc7\u805a\u7c7b\u7b49\u4e8c\u7ea7\u5206\u6790\u5e76\u7f29\u77ed\u8fd0\u884c\u65f6\u95f4\uff0c\u4f46\u5b83\u4e0d\u7b49\u4e8e\u5b8c\u6574\u4ea4\u4ed8\u3002\u5b89\u88c5\u540e\u8fd8\u53ef\u4ee5\u5148\u8dd1 cellranger-arc testrun --id=tiny<\/code>\uff1a\u5b98\u65b9\u79f0\u901a\u5e38\u9700 10\u201420 \u5206\u949f\uff0c\u5728 16 \u6838\u5de5\u4f5c\u7ad9\u4e0a\u6700\u957f\u53ef\u5230\u7ea6 60 \u5206\u949f\u3002\u5b83\u9002\u5408\u68c0\u67e5\u5b89\u88c5\u4e0e\u57fa\u7840\u73af\u5883\uff0c\u4e0d\u80fd\u66ff\u4ee3\u771f\u5b9e\u6837\u672c\u538b\u6d4b\u3002<\/p>\n
\u4ece\u201c\u80fd\u8dd1\u201d\u5230\u201c\u6309\u65f6\u4ea4\u4ed8\u201d\uff0c\u5efa\u8bae\u505a\u8fd9 5 \u6b65<\/h2>\n
    \n
  1. \u5148\u9501\u7248\u672c\u3002<\/strong> \u8bb0\u5f55 ARC\u3001\u53c2\u8003\u5305\u3001\u64cd\u4f5c\u7cfb\u7edf\u548c CPU \u6307\u4ee4\u96c6\uff1bARC 2.2 \u8981\u6c42 64 \u4f4d CentOS\/RedHat 8.0+ \u6216 Ubuntu 18.04+\uff0c\u5e76\u8981\u6c42 AVX\u3002<\/li>\n
  2. \u9009\u4e00\u4e2a\u4ee3\u8868\u6837\u672c\u8bd5\u8dd1\u3002<\/strong> \u8bb0\u5f55\u5cf0\u503c\u5185\u5b58\u3001\u5899\u949f\u65f6\u95f4\u3001\u6700\u5927\u78c1\u76d8\u5360\u7528\u548c I\/O wait\uff0c\u4e0d\u53ea\u770b\u5e73\u5747 CPU\u3002<\/li>\n
  3. \u663e\u5f0f\u9650\u5236\u8d44\u6e90\u3002<\/strong> \u5728\u5171\u4eab\u673a\u4e0a\u59cb\u7ec8\u8bbe\u7f6e --localcores<\/code> \u4e0e --localmem<\/code>\uff1b64 GB \u4f5c\u4e1a\u53ef\u53c2\u8003\u5b98\u65b9\u793a\u4f8b\uff0c\u628a\u5185\u5b58\u4e0a\u9650\u8bbe\u4e3a 57 GB\uff0c\u4e3a\u7cfb\u7edf\u7559\u4f59\u91cf\u3002<\/li>\n
  4. \u6309\u5cf0\u503c\u6392\u5e76\u53d1\u3002<\/strong> \u5148\u6ee1\u8db3\u5355\u4efb\u52a1\u9ad8\u5cf0\uff0c\u518d\u51b3\u5b9a\u80fd\u5426\u5e76\u8dd1\u3002\u4e0d\u8981\u7528\u201c\u603b\u5185\u5b58 \u00f7 64\u201d\u673a\u68b0\u8ba1\u7b97\u5e76\u53d1\u6570\uff0c\u8fd8\u8981\u4e3a\u6587\u4ef6\u7f13\u5b58\u3001\u8c03\u5ea6\u5668\u548c\u5176\u4ed6\u8fdb\u7a0b\u7559\u7a7a\u95f4\u3002<\/li>\n
  5. \u63d0\u524d\u7b97\u78c1\u76d8\u56de\u6536\u3002<\/strong> \u5b98\u65b9\u6700\u4f4e\u53ef\u7528\u78c1\u76d8\u662f 1 TB\uff1bPBMC 10k \u6d4b\u8bd5\u7684\u5b58\u50a8\u9ad8\u6c34\u4f4d\u7ea6 348\u2014400 GB\u3002\u9879\u76ee\u5e94\u5206\u522b\u89c4\u5212\u8f93\u5165\u3001\u8fd0\u884c\u4e2d\u95f4\u6587\u4ef6\u3001BAM\u3001\u5f52\u6863\u548c\u5907\u4efd\uff0c\u5b8c\u6210\u540e\u518d\u6309\u7ea6\u5b9a\u6e05\u7406\u3002<\/li>\n<\/ol>\n
    \u8dd1\u6162\u3001\u88ab\u6740\u3001\u7ed3\u679c\u5f02\u5e38\uff0c\u6392\u67e5\u65b9\u5411\u5e76\u4e0d\u76f8\u540c<\/h2>\n
    \u5982\u679c\u4efb\u52a1\u542f\u52a8\u540e\u5f88\u5feb\u63d0\u793a\u9884\u68c0\u5931\u8d25\uff0c\u4f18\u5148\u67e5 FASTQ \u8def\u5f84\u3001libraries.csv\u3001\u53c2\u8003\u5305\u3001\u7cfb\u7edf\u7248\u672c\u4e0e AVX\uff1b\u53cd\u590d\u5728\u540c\u4e00 stage \u88ab\u7cfb\u7edf\u6740\u6389\uff0c\u5148\u5bf9\u7167\u8c03\u5ea6\u5668\u8bb0\u5f55\u548c dmesg<\/code>\uff0c\u786e\u8ba4\u662f\u5426\u89e6\u53d1 OOM\uff0c\u800c\u4e0d\u662f\u7acb\u523b\u91cd\u8dd1\u3002CPU \u5229\u7528\u7387\u4e0d\u9ad8\u4f46\u5899\u949f\u65f6\u95f4\u6301\u7eed\u589e\u957f\uff0c\u5219\u5e94\u68c0\u67e5\u78c1\u76d8\u541e\u5410\u3001\u5171\u4eab\u5b58\u50a8\u62e5\u5835\u4e0e\u5c0f\u6587\u4ef6\u8bfb\u5199\u3002\u82e5\u6d41\u7a0b\u5b8c\u6210\u4f46\u6709\u6548\u7ec6\u80de\u3001TSS enrichment\u3001FRIP \u6216\u57fa\u56e0\u8868\u8fbe\u8d28\u91cf\u5f02\u5e38\uff0c\u95ee\u9898\u66f4\u53ef\u80fd\u6765\u81ea\u6587\u5e93\u548c\u6570\u636e\u8d28\u91cf\uff0c\u7ee7\u7eed\u52a0\u5185\u5b58\u4e0d\u4f1a\u6539\u5584\u751f\u7269\u5b66\u7ed3\u679c\u3002<\/p>\n
    \u56e0\u6b64\uff0c\u4e00\u6b21\u53ef\u590d\u73b0\u7684\u4ea4\u4ed8\u81f3\u5c11\u8981\u4fdd\u5b58\u547d\u4ee4\u3001\u8f6f\u4ef6\u7248\u672c\u3001\u53c2\u8003\u5305\u540d\u79f0\u3001\u5173\u952e\u65e5\u5fd7\u3001web summary \u548c\u8d44\u6e90\u4f7f\u7528\u8bb0\u5f55\u3002\u4e0b\u6b21\u589e\u52a0\u6837\u672c\u65f6\uff0c\u624d\u80fd\u4f9d\u636e\u771f\u5b9e\u9ad8\u6c34\u4f4d\u6269\u5bb9\uff0c\u800c\u4e0d\u662f\u4ece\u5934\u731c\u914d\u7f6e\u3002\u5bf9\u591a\u4eba\u5171\u7528\u7684\u673a\u5668\uff0c\u8fd8\u5e94\u7ed9\u4efb\u52a1\u76ee\u5f55\u3001\u53c2\u8003\u6570\u636e\u548c\u7ed3\u679c\u5f52\u6863\u8bbe\u6e05\u6670\u6743\u9650\uff0c\u907f\u514d\u201c\u7b97\u5b8c\u4e86\u5374\u627e\u4e0d\u5230\u8f93\u5165\u7248\u672c\u201d\u6216\u8bef\u5220\u4e2d\u95f4\u7ed3\u679c\u3002<\/p>\n
    \u9700\u8981\u6ce8\u610f\u7684\u662f\uff0cCell Ranger ARC \u7684\u4e3b\u8981\u74f6\u9888\u901a\u5e38\u662f CPU\u3001\u5185\u5b58\u548c\u5b58\u50a8 I\/O\uff0c\u4e0d\u662f\u6709 GPU \u5c31\u4e00\u5b9a\u66f4\u5feb\u3002\u82e5\u540e\u7eed\u8fd8\u8981\u505a\u6df1\u5ea6\u5b66\u4e60\u7ec6\u80de\u6ce8\u91ca\u3001\u8de8\u6a21\u6001\u6a21\u578b\u6216\u5927\u89c4\u6a21\u4e8c\u6b21\u5206\u6790\uff0c\u518d\u5355\u72ec\u8bc4\u4f30 GPU \u578b\u53f7\u548c\u663e\u5b58\u66f4\u5408\u7406\u3002\u628a CPU \u6d41\u7a0b\u4e0e GPU \u5de5\u4f5c\u8d1f\u8f7d\u6df7\u5728\u540c\u4e00\u5f20\u914d\u7f6e\u5355\u91cc\uff0c\u5f80\u5f80\u65e2\u589e\u52a0\u6210\u672c\uff0c\u4e5f\u96be\u4ee5\u89e3\u91ca\u5b9e\u9645\u6536\u76ca\u3002<\/p>\n
    \u5355\u673a\u3001\u96c6\u7fa4\u8fd8\u662f\u5916\u5305\u5206\u6790\uff1f\u5148\u770b\u7ba1\u7406\u6210\u672c<\/h2>\n
    \u5355\u4e2a\u6216\u5c11\u91cf\u6837\u672c\uff0c\u7528\u72ec\u5360\u7684\u5927\u5185\u5b58 Linux \u670d\u52a1\u5668\u901a\u5e38\u6700\u76f4\u63a5\uff0c\u65e5\u5fd7\u548c\u76ee\u5f55\u4e5f\u5bb9\u6613\u6392\u67e5\u3002\u5df2\u6709 HPC \u65f6\uff0c\u53ef\u7528\u5355\u8282\u70b9 job submission mode\uff1b10x \u5bf9 ARC \u7684\u591a\u8282\u70b9 cluster mode\u8981\u6c42\u5171\u4eab\u6587\u4ef6\u7cfb\u7edf\u53ca SGE \u6216 LSF\u3002Slurm\/Torque-PBS \u867d\u6709\u7528\u6237\u7528\u4e8e\u5355\u8282\u70b9\u63d0\u4ea4\uff0c\u4f46\u5b98\u65b9\u660e\u786e\u8bf4\u660e\u5e76\u4e0d\u6b63\u5f0f\u652f\u6301\uff0c\u6a21\u677f\u3001\u8def\u5f84\u548c\u8d44\u6e90\u7533\u8bf7\u90fd\u8981\u7531\u672c\u5730\u7ba1\u7406\u5458\u9a8c\u8bc1\u3002<\/p>\n
    \u670d\u52a1\u5668\u79df\u7528\u89e3\u51b3\u7684\u662f\u786c\u4ef6\u4e0e\u4f7f\u7528\u5468\u671f\uff1b\u73af\u5883\u90e8\u7f72\u89e3\u51b3\u7248\u672c\u3001\u53c2\u8003\u5305\u3001\u4f9d\u8d56\u548c\u6d4b\u8bd5\uff1b\u59d4\u6258\u5206\u6790\u5219\u8fd8\u5e94\u5305\u542b\u8f93\u5165\u68c0\u67e5\u3001\u53c2\u6570\u8bb0\u5f55\u3001\u8d28\u63a7\u89e3\u91ca\u4e0e\u7ed3\u679c\u4ea4\u4ed8\u3002\u4e09\u8005\u4e0d\u662f\u540c\u4e00\u9879\u670d\u52a1\u3002\u4e91\u751f\u4fe1\u53ef\u4ee5\u5148\u6839\u636e\u6837\u672c\u6570\u3001FASTQ \u89c4\u6a21\u3001\u4ea4\u4ed8\u7269\u548c\u65f6\u95f4\u7a97\u53e3\u5224\u65ad\u74f6\u9888\uff0c\u518d\u51b3\u5b9a\u662f\u8865\u7b97\u529b\u3001\u642d\u73af\u5883\uff0c\u8fd8\u662f\u628a\u6d41\u7a0b\u6574\u4f53\u6258\u7ba1\uff0c\u907f\u514d\u201c\u673a\u5668\u4e70\u5927\u4e86\u4ecd\u4e0d\u4f1a\u8dd1\u201d\u6216\u201c\u8dd1\u5230\u4e00\u534a\u624d\u53d1\u73b0\u78c1\u76d8\u4e0d\u591f\u201d\u3002<\/p>\n
    \u5982\u679c\u4f60\u6b63\u51c6\u5907 Multiome \u9879\u76ee\uff0c\u53ef\u628a \u6837\u672c\u6570\u3001\u6bcf\u4e2a\u6837\u672c\u4e24\u7c7b FASTQ \u5927\u5c0f\u3001\u9884\u8ba1\u7ec6\u80de\u6570\u3001\u662f\u5426\u4fdd\u7559 BAM\u3001\u671f\u671b\u5b8c\u6210\u65f6\u95f4<\/strong> \u53d1\u6765\uff0c\u6211\u4eec\u53ef\u534f\u52a9\u505a\u8d44\u6e90\u4f30\u7b97\u548c\u6267\u884c\u65b9\u6848\u3002\u5177\u4f53\u8fd0\u884c\u65f6\u95f4\u4ee5\u6570\u636e\u8d28\u91cf\u4e0e\u5b9e\u6d4b\u4e3a\u51c6\uff0c\u4e0d\u627f\u8bfa\u6240\u6709\u6837\u672c\u90fd\u590d\u73b0\u5b98\u65b9\u57fa\u51c6\u3002<\/p>\n
    \u8054\u7cfb\u4e91\u751f\u4fe1\uff0c\u54a8\u8be2 Cell Ranger ARC \u8d44\u6e90\u4e0e\u73af\u5883<\/a><\/p>\n
    \u53c2\u8003\u8d44\u6599<\/h2>\n