{"id":66264,"date":"2026-10-20T09:03:37","date_gmt":"2026-10-20T01:03:37","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66264"},"modified":"2026-10-20T09:03:37","modified_gmt":"2026-10-20T01:03:37","slug":"starsolo-single-cell-rnaseq-resource-planning","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/starsolo-single-cell-rnaseq-resource-planning\/","title":{"rendered":"STARsolo \u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u4e3a\u4ec0\u4e48 16 GB \u80fd\u5efa\u7d22\u5f15\uff0c\u6620\u5c04\u65f6\u5374\u4e0d\u7a33\uff1f\u670d\u52a1\u5668\u8d44\u6e90\u89c4\u5212"},"content":{"rendered":"<div id=\"articleContent\" style=\"max-width:860px;margin:0 auto;color:#1f2937;font-size:17px;line-height:1.85;\">\n<p>\u5f88\u591a\u5355\u7ec6\u80de RNA-seq \u9879\u76ee\u4f1a\u9047\u5230\u4e00\u4e2a\u53cd\u76f4\u89c9\u95ee\u9898\uff1aSTARsolo \u7684\u7a00\u758f\u7d22\u5f15\u53ef\u4ee5\u5728 16 GB \u5185\u5b58\u4e0b\u751f\u6210\uff0c\u771f\u6b63\u628a FASTQ \u9001\u8fdb\u53bb\u540e\u5374\u51fa\u73b0 OOM\u3001\u5171\u4eab\u5185\u5b58\u62a5\u9519\u6216 BAM \u6392\u5e8f\u53d8\u6162\u3002\u539f\u56e0\u901a\u5e38\u4e0d\u662f\u201cSTAR \u4e0d\u80fd\u8dd1\u201d\uff0c\u800c\u662f\u628a\u7d22\u5f15\u6784\u5efa\u3001\u57fa\u56e0\u7ec4\u88c5\u8f7d\u3001\u6bd4\u5bf9\u3001\u7ec6\u80de\u6761\u5f62\u7801\u7ea0\u9519\u548c\u8f93\u51fa\u6392\u5e8f\u5f53\u6210\u4e86\u540c\u4e00\u4e2a\u8d44\u6e90\u9636\u6bb5\u3002<\/p>\n<p>STAR \u5b98\u65b9\u4ed3\u5e93\u5f53\u524d\u516c\u5f00\u7684\u6700\u65b0\u547d\u540d\u7248\u672c\u662f <strong>2.7.11b\uff082024-01-24\uff09<\/strong>\u3002STARsolo \u6587\u6863\u8bf4\u660e\uff0c\u5b83\u76f4\u63a5\u5b8c\u6210\u6761\u5f62\u7801\u7ea0\u9519\u3001\u526a\u63a5\u6bd4\u5bf9\u3001UMI \u53bb\u91cd\u548c\u6bcf\u7ec6\u80de\u5b9a\u91cf\uff0c\u8f93\u51fa\u683c\u5f0f\u53ef\u5bf9\u63a5 10x \u7ed3\u679c\uff1b\u6587\u6863\u8fd8\u7ed9\u51fa\u201c\u7ea6\u4e3a CellRanger 10 \u500d\u901f\u5ea6\u201d\u7684\u603b\u4f53\u63cf\u8ff0\uff0c\u4f46\u8fd9\u4e0d\u662f\u5bf9\u6bcf\u4e2a\u6837\u672c\u3001\u53c2\u6570\u548c\u786c\u4ef6\u7684\u627f\u8bfa\u3002\u771f\u6b63\u8981\u505a\u7a33\u5b9a\u4ea4\u4ed8\uff0c\u5148\u628a\u7d22\u5f15\u5927\u5c0f\u3001\u5171\u4eab\u5185\u5b58\u548c\u8f93\u51fa\u7b56\u7565\u62c6\u5f00\u8ba1\u7b97\u3002<\/p>\n<figure style=\"margin:28px 0;\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/daily-bio-blog-20261020-starsolo-resource-starsolo-resource-poster.png?ssl=1\" alt=\"STARsolo \u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u670d\u52a1\u5668\u8d44\u6e90\u89c4\u5212\" style=\"display:block;width:100%;height:auto;border-radius:14px;\"><figcaption style=\"margin-top:8px;color:#64748b;font-size:14px;text-align:center;\">STARsolo \u8d44\u6e90\u89c4\u5212\uff1a\u628a\u7d22\u5f15\u3001\u5171\u4eab\u5185\u5b58\u3001FASTQ \u987a\u5e8f\u548c\u8f93\u51fa\u7b56\u7565\u5206\u5f00\u8ba1\u7b97\u3002<\/figcaption><\/figure>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u5148\u770b\u5b98\u65b9\u6570\u5b57\uff1a16 GB \u662f\u4e00\u79cd\u53d6\u820d\uff0c\u4e0d\u662f\u5168\u6d41\u7a0b\u914d\u7f6e<\/h2>\n<p>STAR \u624b\u518c\u7ed9\u51fa\u7684\u5178\u578b\u54fa\u4e73\u52a8\u7269\u57fa\u56e0\u7ec4\u7d22\u5f15\u76ee\u5f55\u9700\u8981\u81f3\u5c11 <strong>100 GB \u78c1\u76d8\u7a7a\u95f4<\/strong>\uff1b\u4eba\u7c7b\u57fa\u56e0\u7ec4\u5728\u5171\u4eab\u5185\u5b58\u6a21\u5f0f\u4e0b\u7684 <code>GenomeIndexSize<\/code> \u7ea6\u4e3a <strong>31,000,000,000 bytes\uff08\u7ea6 31 GB\uff09<\/strong>\u3002STARsolo \u6587\u6863\u5efa\u8bae\u52a0\u5165 <code>--genomeSAsparseD 3<\/code> \u751f\u6210\u7a00\u758f\u540e\u7f00\u6570\u7ec4\uff0c\u5b83\u6709\u673a\u4f1a\u628a\u7d22\u5f15\u6784\u5efa\u5185\u5b58\u538b\u5230 <strong>16 GB<\/strong>\uff0c\u4ee3\u4ef7\u662f\u901f\u5ea6\u4e0b\u964d <strong>30%\u201450%<\/strong>\u3002\u56e0\u6b64\uff0c\u201c16 GB \u80fd\u5efa\u7d22\u5f15\u201d\u53ea\u8bf4\u660e\u91c7\u7528\u4e86\u66f4\u7701\u5185\u5b58\u7684\u5efa\u5e93\u7b56\u7565\uff0c\u5e76\u4e0d\u4ee3\u8868 16 GB \u8282\u70b9\u9002\u5408\u540e\u7eed\u591a\u6837\u672c\u6620\u5c04\u3002<\/p>\n<div style=\"overflow-x:auto;margin:18px 0 26px;\">\n<table style=\"width:100%;border-collapse:collapse;min-width:720px;font-size:15px;\">\n<thead style=\"background:#0e5b68;color:#fff;\">\n<tr>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u73af\u8282<\/th>\n<th style=\"text-align: right;\">\u5b98\u65b9\u6216\u6587\u6863\u6570\u5b57<\/th>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u670d\u52a1\u5668\u89c4\u5212\u542b\u4e49<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u6700\u65b0\u547d\u540d\u7248\u672c<\/td>\n<td style=\"text-align: right;\">STAR 2.7.11b<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u7248\u672c\u3001\u7d22\u5f15\u548c\u53c2\u6570\u8981\u6210\u5957\u8bb0\u5f55<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u4eba\u7c7b\u7d22\u5f15\u5171\u4eab\u5185\u5b58\u91cf\u7ea7<\/td>\n<td style=\"text-align: right;\">\u7ea6 31 GB<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u5171\u4eab\u5185\u5b58\u4e0a\u9650\u4e0d\u80fd\u53ea\u6309 16 GB \u914d<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u5178\u578b\u54fa\u4e73\u52a8\u7269\u7d22\u5f15\u78c1\u76d8<\/td>\n<td style=\"text-align: right;\">\u81f3\u5c11 100 GB<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u8fd8\u8981\u7559 FASTA\u3001GTF\u3001\u4e34\u65f6\u76ee\u5f55\u548c\u7ed3\u679c\u7a7a\u95f4<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u7a00\u758f\u7d22\u5f15<\/td>\n<td style=\"text-align: right;\">\u53ef\u9002\u914d 16 GB\uff1b\u901f\u5ea6\u4e0b\u964d 30%\u201450%<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u7701\u5185\u5b58\u6362\u65f6\u95f4\uff0c\u9002\u5408\u5efa\u5e93\u8d44\u6e90\u7d27\u5f20\u573a\u666f<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">STARsolo \u8f93\u5165\u987a\u5e8f<\/td>\n<td style=\"text-align: right;\">cDNA Read2 \u5728\u524d\uff0cBarcode\/UMI Read1 \u5728\u540e<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u987a\u5e8f\u5199\u53cd\u4f1a\u5f97\u5230\u201c\u6d41\u7a0b\u8dd1\u5b8c\u4f46\u7ed3\u679c\u4e0d\u5bf9\u201d<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u6620\u5c04\u9636\u6bb5\uff0c\u5185\u5b58\u5230\u5e95\u82b1\u5728\u54ea\u91cc\uff1f<\/h2>\n<p>\u7b2c\u4e00\u90e8\u5206\u662f genome index\u3002\u9ed8\u8ba4 <code>--genomeLoad NoSharedMemory<\/code> \u4f1a\u8ba9\u6bcf\u4e2a STAR \u8fdb\u7a0b\u81ea\u884c\u8f7d\u5165\u7d22\u5f15\uff1b\u5982\u679c\u540c\u4e00\u53f0\u670d\u52a1\u5668\u8981\u8fde\u7eed\u8dd1\u591a\u4e2a\u6837\u672c\uff0c\u53ef\u4ee5\u7528 <code>LoadAndKeep<\/code> \u628a\u7d22\u5f15\u653e\u5165 Linux \u5171\u4eab\u5185\u5b58\uff0c\u8ba9\u540e\u7eed\u4efb\u52a1\u590d\u7528\u3002\u624b\u518c\u8981\u6c42\u5171\u4eab\u5185\u5b58\u53c2\u6570 <code>kernel.shmmax<\/code> \u548c <code>kernel.shmall<\/code> \u5927\u4e8e\u7d22\u5f15\u91cf\u7ea7\uff1b\u4eba\u7c7b\u53c2\u8003\u82e5\u53ea\u7ed9\u4e86\u51e0 GB <code>\/dev\/shm<\/code>\uff0c\u5c31\u7b97\u7269\u7406\u5185\u5b58\u5f88\u591a\uff0c\u4e5f\u53ef\u80fd\u5728\u542f\u52a8\u65f6\u5931\u8d25\u3002<\/p>\n<p>\u7b2c\u4e8c\u90e8\u5206\u662f\u6620\u5c04\u4e0e\u5355\u7ec6\u80de\u8ba1\u6570\u3002STARsolo \u9700\u8981\u540c\u65f6\u5904\u7406 cDNA\u3001\u7ec6\u80de\u6761\u5f62\u7801\u548c UMI\uff0c\u8fdb\u884c\u7ea0\u9519\u3001\u53bb\u91cd\u548c\u6309\u57fa\u56e0\u805a\u5408\uff1b\u7ec6\u80de\u6570\u3001reads\/cell\u3001\u662f\u5426\u542f\u7528 GeneFull \u6216 Velocyto \u7c7b\u7279\u5f81\uff0c\u90fd\u4f1a\u6539\u53d8\u8fd0\u884c\u4e2d\u95f4\u6570\u636e\u3002\u7b2c\u4e09\u90e8\u5206\u662f\u8f93\u51fa\uff1a\u5982\u679c\u8981\u6c42\u5750\u6807\u6392\u5e8f BAM\u3001\u57fa\u56e0\u7ec4\u6d4f\u89c8\u6216\u4e0b\u6e38\u91cd\u6bd4\u5bf9\uff0c\u4e34\u65f6\u76ee\u5f55\u548c\u6392\u5e8f\u9636\u6bb5\u4f1a\u7ee7\u7eed\u5403\u78c1\u76d8\u4e0e\u5185\u5b58\u3002\u53ea\u76d1\u63a7\u201c\u7d22\u5f15\u6587\u4ef6\u5927\u5c0f\u201d\uff0c\u4f1a\u6f0f\u6389\u5cf0\u503c\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u4e00\u6761\u53ef\u590d\u7528\u7684\u547d\u4ee4\uff0c\u5148\u628a\u5173\u952e\u8fb9\u754c\u5199\u6e05\u695a<\/h2>\n<pre style=\"overflow-x:auto;padding:18px;background:#102f47;color:#f8fafc;border-radius:12px;line-height:1.55;\"><code class=\"language-bash\">STAR --genomeDir \/data\/star\/grch38 \\\r\n  --readFilesIn sample_R2.fastq.gz sample_R1.fastq.gz \\\r\n  --readFilesCommand zcat \\\r\n  --runThreadN 32 \\\r\n  --soloType CB_UMI_Simple \\\r\n  --soloCBwhitelist \/data\/10x\/3M-february-2018.txt \\\r\n  --soloUMIlen 12 \\\r\n  --genomeLoad NoSharedMemory \\\r\n  --outTmpDir \/scratch\/sample01 \\\r\n  --outFileNamePrefix \/data\/result\/sample01_\r\n<\/code><\/pre>\n<p>\u8fd9\u6761\u547d\u4ee4\u4e2d\u7684 <code>Read2<\/code> \u662f cDNA\uff0c<code>Read1<\/code> \u662f barcode\/UMI\uff1b10x V3 \u7b49\u5316\u5b66\u7248\u672c\u8fd8\u8981\u6838\u5bf9 whitelist \u548c UMI \u957f\u5ea6\u3002\u82e5\u76ee\u6807\u662f\u5c3d\u91cf\u8d34\u8fd1 CellRanger 4\/5 \u7684\u8ba1\u6570\u903b\u8f91\uff0cSTARsolo \u6587\u6863\u5217\u51fa\u4e86 <code>--soloCBmatchWLtype 1MM_multi_Nbase_pseudocounts<\/code>\u3001<code>--soloUMIfiltering MultiGeneUMI_CR<\/code> \u548c <code>--soloUMIdedup 1MM_CR<\/code> \u7b49\u53c2\u6570\uff0c\u4f46\u201c\u63a5\u8fd1\u201d\u4e0d\u7b49\u4e8e\u6240\u6709 QC \u6307\u6807\u548c\u8fc7\u6ee4\u7ed3\u679c\u5b8c\u5168\u76f8\u540c\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u670d\u52a1\u5668\u600e\u4e48\u914d\uff0c\u5173\u952e\u770b\u662f\u5426\u5171\u4eab\u548c\u662f\u5426\u4fdd\u7559 BAM<\/h2>\n<p>\u4e2a\u4eba\u5355\u6837\u672c\u6d4b\u8bd5\uff0c\u53ef\u4ee5\u4ece <strong>64 GB \u5185\u5b58\u300116\u201432 \u6838\u3001100 GB \u4ee5\u4e0a\u7d22\u5f15\u76d8<\/strong>\u4f5c\u4e3a\u5de5\u7a0b\u8d77\u70b9\uff1b\u8fd9\u662f\u4fbf\u4e8e\u52a0\u8f7d\u4eba\u7c7b\u7d22\u5f15\u5e76\u7559\u51fa\u7cfb\u7edf\u4f59\u91cf\u7684\u5b9e\u7528\u5efa\u8bae\uff0c\u4e0d\u662f STAR \u5b98\u65b9\u786c\u95e8\u69db\u3002\u591a\u4eba\u5e76\u884c\u6216\u8981\u8f93\u51fa\u5750\u6807\u6392\u5e8f BAM\uff0c\u66f4\u5efa\u8bae\u4ece <strong>128 GB \u5185\u5b58\u3001NVMe \u4e34\u65f6\u76d8<\/strong>\u8bc4\u4f30\u3002\u82e5\u53ea\u662f\u505a\u77e9\u9635\u3001\u4e0d\u4fdd\u7559 BAM\uff0c\u53ef\u628a\u7a7a\u95f4\u548c\u6392\u5e8f\u538b\u529b\u964d\u4e0b\u6765\uff1b\u82e5\u8981\u590d\u73b0\u3001\u6d4f\u89c8\u6216\u4e8c\u6b21\u6bd4\u5bf9\uff0c\u5219\u5e94\u628a BAM\u3001\u4e34\u65f6\u6587\u4ef6\u548c\u5907\u4efd\u5206\u5f00\u9884\u7559\u3002<\/p>\n<p>\u8fd8\u8981\u628a\u201c\u6837\u672c\u5927\u5c0f\u201d\u548c\u201c\u5e76\u53d1\u6570\u201d\u653e\u8fdb\u540c\u4e00\u4e2a\u9884\u7b97\u3002\u5355\u4e2a\u6837\u672c 100 GB FASTQ\uff0c\u5e76\u4e0d\u7b49\u4e8e\u53ea\u9700\u8981 100 GB \u7a7a\u95f4\uff1a\u89e3\u538b\u3001\u4e34\u65f6\u6392\u5e8f\u3001\u65e5\u5fd7\u548c\u5931\u8d25\u91cd\u8dd1\u90fd\u53ef\u80fd\u4ea7\u751f\u989d\u5916\u526f\u672c\u3002\u82e5\u4e24\u4e09\u4e2a\u6837\u672c\u540c\u65f6\u542f\u52a8\uff0c\u5373\u4f7f\u6bcf\u4e2a\u4efb\u52a1\u90fd\u80fd\u5728 64 GB \u4e0a\u5355\u72ec\u8fd0\u884c\uff0c\u4e5f\u53ef\u80fd\u5728\u5cf0\u503c\u9636\u6bb5\u4e92\u76f8\u4e89\u62a2\u5185\u5b58\u548c NVMe \u541e\u5410\u3002\u66f4\u7a33\u59a5\u7684\u505a\u6cd5\u662f\u5148\u7528\u4e00\u4e2a\u4ee3\u8868\u6837\u672c\u8bb0\u5f55 <code>MaxRSS<\/code>\u3001\u4e34\u65f6\u76ee\u5f55\u5cf0\u503c\u548c\u6bcf\u5206\u949f I\/O\uff0c\u518d\u6309\u8ba1\u5212\u5e76\u53d1\u6570\u52a0\u5b89\u5168\u4f59\u91cf\uff0c\u800c\u4e0d\u662f\u76f4\u63a5\u7528\u201c\u6838\u6570\u00d7\u4efb\u52a1\u6570\u201d\u4f30\u7b97\u670d\u52a1\u5668\u3002<\/p>\n<p>\u5982\u679c\u56e2\u961f\u9700\u8981\u5468\u671f\u6027\u590d\u7528\u540c\u4e00\u53c2\u8003\uff0c\u5efa\u8bae\u628a\u53c2\u8003\u7d22\u5f15\u505a\u6210\u53ea\u8bfb\u7248\u672c\u76ee\u5f55\uff0c\u7ed9\u6bcf\u4e2a\u9879\u76ee\u5355\u72ec\u4fdd\u5b58\u53c2\u6570\u3001\u8f6f\u4ef6\u7248\u672c\u548c\u65e5\u5fd7\u3002\u8fd9\u6837\u65e2\u80fd\u907f\u514d\u7528\u6237\u8bef\u6539\u7d22\u5f15\uff0c\u4e5f\u65b9\u4fbf\u5728\u5347\u7ea7 STAR \u6216\u66ff\u6362 GTF \u65f6\u8fdb\u884c A\/B \u5bf9\u7167\u3002\u670d\u52a1\u5668\u79df\u8d41\u65f6\uff0c\u5e94\u63d0\u524d\u786e\u8ba4\u662f\u5426\u5141\u8bb8\u8c03\u6574 <code>\/dev\/shm<\/code>\u3001\u662f\u5426\u6709\u4f5c\u4e1a\u961f\u5217\u3001\u4e34\u65f6\u76d8\u662f\u672c\u5730 NVMe \u8fd8\u662f\u7f51\u7edc\u76d8\uff1b\u8fd9\u4e9b\u6761\u4ef6\u4f1a\u76f4\u63a5\u51b3\u5b9a <code>LoadAndKeep<\/code> \u80fd\u5426\u843d\u5730\u3002<\/p>\n<p>\u8d44\u6e90\u8bc4\u4f30\u6700\u597d\u8f93\u51fa\u4e00\u9875\u201c\u8fd0\u884c\u5361\u7247\u201d\uff1a\u8bb0\u5f55\u53c2\u8003\u7248\u672c\u3001STAR \u7248\u672c\u3001\u6838\u5fc3\u53c2\u6570\u3001\u9884\u8ba1\u5e76\u53d1\u3001\u78c1\u76d8\u914d\u989d\u3001\u5931\u8d25\u91cd\u8bd5\u65b9\u5f0f\u548c\u7ed3\u679c\u4fdd\u7559\u5468\u671f\u3002\u4ea4\u63a5\u65f6\u628a\u8fd9\u5f20\u5361\u7247\u4e0e <code>Log.out<\/code>\u3001\u547d\u4ee4\u884c\u548c\u8f6f\u4ef6\u5bb9\u5668\u6807\u7b7e\u4e00\u8d77\u5f52\u6863\uff0c\u540e\u7eed\u6362\u673a\u5668\u6216\u6362\u5206\u6790\u4eba\u5458\u65f6\uff0c\u6392\u67e5\u6210\u672c\u4f1a\u660e\u663e\u4e0b\u964d\u3002<\/p>\n<p>\u5171\u4eab\u670d\u52a1\u5668\u4e0a\uff0c\u8fde\u7eed\u8fd0\u884c\u591a\u4e2a\u6837\u672c\u65f6\u53ef\u4ee5\u8bc4\u4f30 <code>LoadAndKeep<\/code>\uff1a\u7d22\u5f15\u53ea\u52a0\u8f7d\u4e00\u6b21\uff0c\u540e\u7eed\u4efb\u52a1\u590d\u7528\u540c\u4e00\u4efd\u53ea\u8bfb\u5185\u5b58\uff1b\u4f46\u5fc5\u987b\u548c\u7ba1\u7406\u5458\u786e\u8ba4 <code>\/dev\/shm<\/code>\u3001<code>kernel.shmmax<\/code>\u3001<code>ulimit<\/code> \u548c\u4f5c\u4e1a\u8c03\u5ea6\u7b56\u7565\u3002\u6ca1\u6709\u5171\u4eab\u5185\u5b58\u6761\u4ef6\u65f6\uff0c\u660e\u786e\u4f7f\u7528 <code>NoSharedMemory<\/code>\uff0c\u5e76\u628a\u6bcf\u4e2a\u4efb\u52a1\u7684 <code>--outTmpDir<\/code> \u653e\u5728\u672c\u5730 NVMe\uff0c\u907f\u514d\u591a\u4e2a\u4efb\u52a1\u540c\u65f6\u5199 NFS \u5c0f\u6587\u4ef6\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">5 \u4e2a\u6700\u5bb9\u6613\u88ab\u5ffd\u7565\u7684\u6392\u67e5\u70b9<\/h2>\n<ol>\n<li><strong>\u7d22\u5f15\u548c STAR \u7248\u672c\u662f\u5426\u914d\u5957\u3002<\/strong> \u53c2\u8003 FASTA\u3001GTF\u3001<code>sjdbOverhang<\/code>\u3001STAR \u7248\u672c\u53d8\u5316\u540e\uff0c\u5e94\u91cd\u65b0\u6838\u5bf9\u7d22\u5f15\uff0c\u4e0d\u8981\u628a\u65e7\u76ee\u5f55\u76f4\u63a5\u590d\u5236\u7ed9\u65b0\u4e8c\u8fdb\u5236\u3002<\/li>\n<li><strong>\u5171\u4eab\u5185\u5b58\u5931\u8d25\u4e0d\u7b49\u4e8e\u7269\u7406\u5185\u5b58\u4e0d\u8db3\u3002<\/strong> \u67e5\u770b <code>Log.out<\/code>\u3001<code>\/dev\/shm<\/code>\u3001<code>ipcs<\/code> \u548c\u7cfb\u7edf\u9650\u5236\uff1b\u6709\u65f6\u662f <code>shmmax<\/code> \u592a\u5c0f\uff0c\u6539\u6210 <code>NoSharedMemory<\/code> \u6216\u8c03\u6574\u7ba1\u7406\u5458\u914d\u7f6e\u624d\u662f\u6b63\u786e\u65b9\u5411\u3002<\/li>\n<li><strong>FASTQ \u987a\u5e8f\u548c\u5316\u5b66\u7248\u672c\u3002<\/strong> Read1\/Read2 \u5199\u53cd\u3001whitelist \u7528\u9519\u7248\u672c\uff0c\u53ef\u80fd\u8ba9\u6d41\u7a0b\u5b8c\u6210\u4f46\u6709\u6548\u7ec6\u80de\u548c\u5339\u914d\u7387\u5f02\u5e38\u3002<\/li>\n<li><strong>\u7d22\u5f15\u6784\u5efa\u4e0d\u8981\u548c\u6b63\u5f0f\u6620\u5c04\u62a2\u8282\u70b9\u3002<\/strong> <code>--genomeSAsparseD 3<\/code> \u53ef\u4ee5\u964d\u4f4e\u5efa\u5e93\u5185\u5b58\uff0c\u4f46 30%\u201450% \u7684\u901f\u5ea6\u635f\u5931\u5e94\u7eb3\u5165\u6392\u671f\uff1b\u6700\u597d\u4e00\u6b21\u6784\u5efa\u3001\u591a\u6b21\u590d\u7528\u3002<\/li>\n<li><strong>\u628a\u201c\u8dd1\u5b8c\u201d\u4e0e\u201c\u7ed3\u679c\u53ef\u6bd4\u201d\u5206\u5f00\u3002<\/strong> \u82e5\u9700\u8981\u548c CellRanger \u5bf9\u7167\uff0c\u5fc5\u987b\u56fa\u5b9a GTF\u3001whitelist\u3001\u8fc7\u6ee4\u53c2\u6570\u548c\u8f93\u51fa\u7c7b\u578b\uff1b\u4e0d\u540c\u7248\u672c\u548c\u53c2\u6570\u7684\u77e9\u9635\u4e0d\u80fd\u53ea\u770b\u6587\u4ef6\u540d\u5c31\u76f4\u63a5\u5408\u5e76\u3002<\/li>\n<\/ol>\n<p>\u5982\u679c\u4f60\u7684 STARsolo \u4efb\u52a1\u53cd\u590d\u5361\u5728\u7d22\u5f15\u3001\u5171\u4eab\u5185\u5b58\u3001UMI \u7ea0\u9519\u6216 BAM \u6392\u5e8f\uff0c\u53ef\u4ee5\u51c6\u5907 STAR \u7248\u672c\u3001\u53c2\u8003\u5305\u3001\u7ec6\u80de\u6570\u3001reads\/cell\u3001FASTQ \u5927\u5c0f\u3001\u76ee\u6807\u8f93\u51fa\u548c\u62a5\u9519\u9636\u6bb5\u3002\u4e91\u751f\u4fe1\u4f1a\u5148\u5224\u65ad\u662f\u8d44\u6e90\u4e0d\u8db3\u3001\u5171\u4eab\u5185\u5b58\u9650\u5236\u3001\u53c2\u6570\/\u5316\u5b66\u7248\u672c\u4e0d\u5339\u914d\uff0c\u8fd8\u662f\u8f93\u51fa\u7b56\u7565\u9020\u6210\u7684 I\/O \u74f6\u9888\uff0c\u518d\u6c9f\u901a\u670d\u52a1\u5668\u79df\u8d41\u3001\u73af\u5883\u90e8\u7f72\u6216\u59d4\u6258\u5206\u6790\u7684\u9002\u7528\u8303\u56f4\u3002\u5177\u4f53\u8017\u65f6\u548c\u5cf0\u503c\u4ee5\u4ee3\u8868\u6837\u672c\u5b9e\u6d4b\u4e3a\u51c6\uff0c\u4e0d\u627f\u8bfa\u590d\u73b0\u6587\u6863\u4e2d\u7684\u901f\u5ea6\u63cf\u8ff0\u3002<\/p>\n<p><a href=\"https:\/\/work.weixin.qq.com\/kfid\/kfcb05492f29d4d6064\" data-ysx-event=\"contact_click\" style=\"display:inline-block;padding:13px 20px;border-radius:10px;background:#e36e2e;color:#fff;text-decoration:none;font-weight:800;\">\u8054\u7cfb\u4e91\u751f\u4fe1\uff0c\u54a8\u8be2 STARsolo \u8d44\u6e90\u4e0e\u73af\u5883<\/a><\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u53c2\u8003\u8d44\u6599<\/h2>\n<ul>\n<li><a href=\"https:\/\/github.com\/alexdobin\/STAR\">STAR \u5b98\u65b9\u4ed3\u5e93\u4e0e 2.7.11b \u53d1\u5e03\u4fe1\u606f<\/a><\/li>\n<li><a href=\"https:\/\/github.com\/alexdobin\/STAR\/blob\/master\/docs\/STARsolo.md\">STARsolo \u5b98\u65b9\u6587\u6863\uff1a\u7a00\u758f\u7d22\u5f15\u300110x \u8f93\u5165\u4e0e CellRanger \u5bf9\u9f50\u53c2\u6570<\/a><\/li>\n<li><a href=\"https:\/\/github.com\/alexdobin\/STAR\/blob\/master\/extras\/doc-latex\/STARmanual.tex\">STAR \u5b98\u65b9\u624b\u518c\uff1a\u7d22\u5f15\u78c1\u76d8\u3001\u5171\u4eab\u5185\u5b58\u548c\u8fd0\u884c\u53c2\u6570<\/a><\/li>\n<\/ul>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>STARsolo \u7684\u7a00\u758f\u7d22\u5f15\u53ef\u4ee5\u9002\u914d 16 GB \u5185\u5b58\uff0c\u4f46\u4eba\u7c7b\u7d22\u5f15\u5171\u4eab\u5185\u5b58\u91cf\u7ea7\u7ea6 31 GB\u3001\u5178\u578b\u54fa\u4e73\u52a8\u7269\u7d22\u5f15\u81f3\u5c11\u9700\u8981 100 GB \u78c1\u76d8\u3002\u672c\u6587\u62c6\u89e3\u7d22\u5f15\u3001\u5171\u4eab\u5185\u5b58\u3001FASTQ \u987a\u5e8f\u4e0e\u5e76\u53d1\u89c4\u5212\u3002<\/p>\n","protected":false},"author":1,"featured_media":66263,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[71,75,72],"tags":[80,241,399,400,90,79,275,352],"class_list":["post-66264","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-single-cell","category-compute-environment","category-transcriptomics","tag-rna-seq","tag-star","tag-starsolo","tag-400","tag-90","tag-79","tag-275","tag-352"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/daily-bio-blog-20261020-starsolo-resource-starsolo-resource-poster.png?fit=1080%2C1350&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66264","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=66264"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66264\/revisions"}],"predecessor-version":[{"id":66265,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66264\/revisions\/66265"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/66263"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=66264"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=66264"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=66264"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}