{"id":66270,"date":"2026-10-21T09:08:35","date_gmt":"2026-10-21T01:08:35","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66270"},"modified":"2026-10-21T09:08:35","modified_gmt":"2026-10-21T01:08:35","slug":"kraken2-metagenome-database-memory-planning","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/kraken2-metagenome-database-memory-planning\/","title":{"rendered":"Kraken2 \u660e\u660e\u5f88\u5feb\uff0c\u4e3a\u4ec0\u4e48 32 GB \u670d\u52a1\u5668\u8fd8\u662f\u5361\u6b7b\uff1f\u6570\u636e\u5e93\u5927\u5c0f\u624d\u662f\u5173\u952e"},"content":{"rendered":"<div id=\"articleContent\" style=\"max-width:860px;margin:0 auto;color:#1f2937;font-size:17px;line-height:1.85;\">\n<p>Kraken2 \u5e38\u88ab\u79f0\u4e3a\u201c\u5f88\u5feb\u7684\u5b8f\u57fa\u56e0\u7ec4\u7269\u79cd\u5206\u7c7b\u5de5\u5177\u201d\uff0c\u4e8e\u662f\u5f88\u591a\u4eba\u5148\u88c5\u8f6f\u4ef6\u3001\u518d\u628a\u7ebf\u7a0b\u62c9\u6ee1\uff0c\u6700\u540e\u5374\u5361\u5728\u6570\u636e\u5e93\u52a0\u8f7d\uff1a\u8fdb\u7a0b\u88ab\u7cfb\u7edf\u6740\u6389\u3001<code>hash.k2d<\/code> \u8bfb\u4e0d\u8fdb\u53bb\uff0c\u6216\u8005\u540c\u4e00\u6279 FASTQ \u5728\u672c\u5730\u76d8\u5341\u51e0\u5206\u949f\u5b8c\u6210\uff0c\u6362\u5230\u5171\u4eab\u5b58\u50a8\u540e\u901f\u5ea6\u7a81\u7136\u6389\u5230\u5e95\u3002\u771f\u6b63\u51b3\u5b9a\u8d44\u6e90\u7684\u5f80\u5f80\u4e0d\u662f Kraken2 \u7a0b\u5e8f\u672c\u8eab\uff0c\u800c\u662f\u4f60\u9009\u62e9\u4e86\u54ea\u4e00\u5957\u6570\u636e\u5e93\u3001\u6570\u636e\u5e93\u653e\u5728\u54ea\u91cc\uff0c\u4ee5\u53ca\u540c\u65f6\u542f\u52a8\u4e86\u51e0\u4e2a\u4efb\u52a1\u3002<\/p>\n<p>\u622a\u81f3\u53d1\u7a3f\uff0cKraken2 \u5b98\u65b9 GitHub Releases \u5c06 <strong>v2.17.2<\/strong> \u6807\u4e3a\u6700\u65b0\u7248\u672c\u3002\u66f4\u503c\u5f97\u5173\u6ce8\u7684\u662f\u6570\u636e\u5e93\u589e\u957f\uff1aLangmead Lab \u7684\u516c\u5f00\u7d22\u5f15\u9875\u663e\u793a\uff0c2026-02-26 \u7684 PlusPFP \u7d22\u5f15\u8fbe\u5230 <strong>221.8 GB<\/strong>\uff0c\u800c\u540c\u65e5\u7684 Viral \u7d22\u5f15\u53ea\u6709 <strong>0.6 GB<\/strong>\u3002\u4e24\u8005\u90fd\u80fd\u88ab\u79f0\u4e3a\u201cKraken2 \u6570\u636e\u5e93\u201d\uff0c\u8d44\u6e90\u9700\u6c42\u5374\u76f8\u5dee\u7ea6 <strong>370 \u500d<\/strong>\u3002<\/p>\n<figure style=\"margin:28px 0;\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/daily-bio-blog-20261021-kraken2-memory-kraken2-memory-poster.png?ssl=1\" alt=\"Kraken2 \u5b8f\u57fa\u56e0\u7ec4\u6570\u636e\u5e93\u4e0e\u670d\u52a1\u5668\u5185\u5b58\u89c4\u5212\" style=\"display:block;width:100%;height:auto;border-radius:14px;\"><figcaption style=\"margin-top:8px;color:#64748b;font-size:14px;text-align:center;\">Kraken2 \u8d44\u6e90\u89c4\u5212\uff1a\u6570\u636e\u5e93\u8986\u76d6\u3001\u7d22\u5f15\u5927\u5c0f\u3001\u5185\u5b58\u3001NVMe \u4e0e\u5e76\u53d1\u5fc5\u987b\u4e00\u8d77\u8ba1\u7b97\u3002<\/figcaption><\/figure>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u5148\u770b\u6570\u636e\u5e93\uff1a\u538b\u7f29\u5305\u5927\u5c0f\u4e0d\u7b49\u4e8e\u8fd0\u884c\u5185\u5b58<\/h2>\n<p>\u516c\u5f00\u7d22\u5f15\u9875\u540c\u65f6\u5217\u51fa archive size \u4e0e index size\u3002\u4e0b\u8f7d\u7684\u662f\u538b\u7f29\u5305\uff0c\u5206\u7c7b\u65f6\u771f\u6b63\u9891\u7e41\u968f\u673a\u8bbf\u95ee\u7684\u662f\u5c55\u5f00\u540e\u7684\u7d22\u5f15\uff1bKraken2 \u5b98\u65b9\u624b\u518c\u8bf4\u660e\uff0c\u4e3a\u9ad8\u6548\u8fd0\u884c\uff0c\u9700\u8981\u6709\u8db3\u591f\u7a7a\u95f2\u5185\u5b58\u5bb9\u7eb3\u6570\u636e\u5e93\uff0c\u4e3b\u8981\u662f compact hash table\u3002\u53ea\u6309\u4e0b\u8f7d\u6587\u4ef6\u5927\u5c0f\u914d\u76d8\u548c\u5185\u5b58\uff0c\u89e3\u538b\u540e\u5c31\u53ef\u80fd\u7acb\u523b\u8d85\u9884\u7b97\u3002<\/p>\n<div style=\"overflow-x:auto;margin:18px 0 26px;\">\n<table style=\"width:100%;border-collapse:collapse;min-width:720px;font-size:15px;\">\n<thead style=\"background:#0e5b68;color:#fff;\">\n<tr>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u6570\u636e\u5e93\uff08\u516c\u5f00\u65e5\u671f\uff09<\/th>\n<th style=\"text-align: right;\">\u538b\u7f29\u5305<\/th>\n<th style=\"text-align: right;\">\u7d22\u5f15\u5927\u5c0f<\/th>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u8d44\u6e90\u5224\u65ad<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">Viral\uff082026-02-26\uff09<\/td>\n<td style=\"text-align: right;\">0.5 GB<\/td>\n<td style=\"text-align: right;\">0.6 GB<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u75c5\u6bd2\u4e13\u9879\uff0c\u4e0d\u80fd\u66ff\u4ee3\u7efc\u5408\u5e93<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">PlusPF-8\uff082026-02-26\uff09<\/td>\n<td style=\"text-align: right;\">5.5 GB<\/td>\n<td style=\"text-align: right;\">7.5 GB<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u53d7\u9650\u5e93\uff0c\u9002\u5408\u4f4e\u5185\u5b58\u8bd5\u8dd1<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">PlusPF-16\uff082026-02-26\uff09<\/td>\n<td style=\"text-align: right;\">11.2 GB<\/td>\n<td style=\"text-align: right;\">14.9 GB<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">32 GB \u4e3b\u673a\u66f4\u5bb9\u6613\u7559\u51fa\u7cfb\u7edf\u4f59\u91cf<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">Standard\uff082025-07-14\uff09<\/td>\n<td style=\"text-align: right;\">70.8 GB<\/td>\n<td style=\"text-align: right;\">91.8 GB<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u5b9e\u7528\u8d77\u70b9\u901a\u5e38\u8981\u8bc4\u4f30 128 GB \u5185\u5b58<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">PlusPFP\uff082026-02-26\uff09<\/td>\n<td style=\"text-align: right;\">164.4 GB<\/td>\n<td style=\"text-align: right;\">221.8 GB<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u591a\u6837\u672c\u4e0e\u5e76\u53d1\u9700\u8bc4\u4f30 256 GB \u4ee5\u4e0a\u8282\u70b9<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>\u6700\u540e\u4e24\u884c\u7684\u5185\u5b58\u5efa\u8bae\u662f\u5de5\u7a0b\u8d77\u70b9\uff0c\u4e0d\u662f\u5b98\u65b9\u786c\u95e8\u69db\u3002\u8fd8\u8981\u7ed9\u64cd\u4f5c\u7cfb\u7edf\u3001\u8f93\u5165\u7f13\u51b2\u3001\u62a5\u544a\u751f\u6210\u548c\u5e76\u53d1\u4efb\u52a1\u7559\u4f59\u91cf\u3002\u6570\u636e\u5e93\u5185\u5bb9\u4e5f\u4e0d\u540c\uff1aPlusPFP \u6bd4 Standard \u589e\u52a0 RefSeq \u539f\u751f\u751f\u7269\u3001\u771f\u83cc\u548c\u690d\u7269\uff1b\u82e5\u7814\u7a76\u95ee\u9898\u53ea\u5173\u5fc3\u75c5\u6bd2\uff0c\u52a0\u8f7d\u7efc\u5408\u5e93\u4e0d\u4ec5\u6d6a\u8d39\u8d44\u6e90\uff0c\u8fd8\u4f1a\u6269\u5927\u9700\u8981\u89e3\u91ca\u7684\u5206\u7c7b\u7a7a\u95f4\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\"><code>--memory-mapping<\/code> \u80fd\u7701\u5185\u5b58\uff0c\u4f46\u4e0d\u662f\u201c\u96f6\u5185\u5b58\u8fd0\u884c\u201d<\/h2>\n<p>\u9ed8\u8ba4\u60c5\u51b5\u4e0b\uff0cKraken2 \u628a\u6570\u636e\u5e93\u8f7d\u5165\u8fdb\u7a0b\u672c\u5730\u5185\u5b58\u3002<code>--memory-mapping<\/code> \u8ba9\u7a0b\u5e8f\u901a\u8fc7\u5185\u5b58\u6620\u5c04\u8bbf\u95ee\u6570\u636e\u5e93\uff0c\u591a\u4e2a\u8fdb\u7a0b\u6709\u673a\u4f1a\u590d\u7528\u64cd\u4f5c\u7cfb\u7edf\u9875\u7f13\u5b58\uff0c\u907f\u514d\u6bcf\u4e2a\u4efb\u52a1\u90fd\u590d\u5236\u4e00\u4efd\u5b8c\u6574\u7d22\u5f15\u3002\u5b83\u9002\u5408\u4e00\u53f0\u670d\u52a1\u5668\u4e0a\u8fde\u7eed\u5904\u7406\u591a\u6837\u672c\uff0c\u4f46\u6027\u80fd\u53d6\u51b3\u4e8e\u9875\u9762\u662f\u5426\u5df2\u7ecf\u7f13\u5b58\uff0c\u4ee5\u53ca\u5e95\u5c42\u5b58\u50a8\u7684\u968f\u673a\u8bfb\u53d6\u80fd\u529b\u3002<\/p>\n<p>\u5b98\u65b9\u624b\u518c\u660e\u786e\u5efa\u8bae\u5c3d\u53ef\u80fd\u4e0d\u8981\u628a Kraken2 \u6570\u636e\u5e93\u653e\u5728 NFS\u3002\u6570\u636e\u5e93\u8bbf\u95ee\u5e76\u4e0d\u662f\u4e00\u6b21\u987a\u5e8f\u8bfb\u5b8c\uff1b\u51b7\u7f13\u5b58\u65f6\uff0c\u7f51\u7edc\u76d8\u5ef6\u8fdf\u53ef\u80fd\u8ba9\u201cCPU \u8fd8\u6709\u5f88\u591a\u7a7a\u95f2\u201d\u4e0e\u201c\u4efb\u52a1\u5f88\u6162\u201d\u540c\u65f6\u51fa\u73b0\u3002\u66f4\u7a33\u59a5\u7684\u7ed3\u6784\u662f\uff1a\u6570\u636e\u5e93\u653e\u672c\u5730 NVMe\uff0c\u539f\u59cb FASTQ \u548c\u6700\u7ec8\u62a5\u544a\u653e\u5bb9\u91cf\u76d8\uff0c\u4efb\u52a1\u5f00\u59cb\u524d\u786e\u8ba4\u5269\u4f59\u5185\u5b58\u4e0e\u78c1\u76d8\u541e\u5410\u3002<\/p>\n<pre style=\"overflow-x:auto;padding:18px;background:#102f47;color:#f8fafc;border-radius:12px;line-height:1.55;\"><code class=\"language-bash\">kraken2 --db \/nvme\/db\/k2_pluspf_16_20260226 \\\r\n  --paired sample_R1.fastq.gz sample_R2.fastq.gz \\\r\n  --gzip-compressed --threads 24 \\\r\n  --memory-mapping \\\r\n  --report sample.kreport \\\r\n  --output sample.kraken\r\n<\/code><\/pre>\n<p>\u547d\u4ee4\u80fd\u8dd1\u4e0d\u4ee3\u8868\u7ed3\u679c\u5df2\u7ecf\u53ef\u6bd4\u3002\u6570\u636e\u5e93\u540d\u79f0\u3001\u53d1\u5e03\u65e5\u671f\u3001Kraken2 \u7248\u672c\u3001\u7f6e\u4fe1\u5ea6\u9608\u503c\u548c\u662f\u5426\u4f7f\u7528 Bracken\uff0c\u90fd\u5e94\u4e0e\u7ed3\u679c\u4e00\u8d77\u8bb0\u5f55\u3002\u540c\u4e00\u9879\u76ee\u4e2d\u9014\u6362\u5e93\uff0c\u4f1a\u540c\u65f6\u6539\u53d8\u53ef\u68c0\u51fa\u7684\u7269\u79cd\u8303\u56f4\u3001minimizer \u96c6\u5408\u548c\u4e30\u5ea6\u4f30\u8ba1\u57fa\u7840\uff0c\u4e0d\u80fd\u628a\u524d\u540e\u4e24\u6279\u62a5\u544a\u76f4\u63a5\u62fc\u63a5\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u7ebf\u7a0b\u8d8a\u591a\u8d8a\u5feb\uff1f\u5148\u770b\u74f6\u9888\u5728 CPU \u8fd8\u662f I\/O<\/h2>\n<p><code>--threads 24<\/code> \u53ea\u8868\u793a\u6700\u591a\u4f7f\u7528\u591a\u5c11\u8ba1\u7b97\u7ebf\u7a0b\uff0c\u4e0d\u4f1a\u81ea\u52a8\u89e3\u51b3\u6570\u636e\u5e93\u672a\u7f13\u5b58\u3001\u7f51\u7edc\u76d8\u5ef6\u8fdf\u6216 gzip \u89e3\u538b\u4e89\u7528\u3002\u7b2c\u4e00\u6b21\u8fd0\u884c\u5927\u578b\u7d22\u5f15\u65f6\uff0c\u7cfb\u7edf\u8981\u628a\u5927\u91cf\u9875\u9762\u4ece\u78c1\u76d8\u8bfb\u5165\u5185\u5b58\uff1b\u6b64\u65f6 CPU \u5229\u7528\u7387\u53ef\u80fd\u4e0d\u9ad8\uff0c\u4f46\u78c1\u76d8\u961f\u5217\u6301\u7eed\u589e\u957f\u3002\u7b2c\u4e8c\u4e2a\u6837\u672c\u82e5\u590d\u7528\u70ed\u9875\u7f13\u5b58\uff0c\u901f\u5ea6\u5f80\u5f80\u660e\u663e\u4e0d\u540c\u3002\u56e0\u6b64 benchmark \u81f3\u5c11\u8981\u5206\u522b\u8bb0\u5f55\u51b7\u7f13\u5b58\u4e0e\u70ed\u7f13\u5b58\uff0c\u4e0d\u8981\u53ea\u6311\u6700\u5feb\u7684\u4e00\u6b21\u4f5c\u4e3a\u670d\u52a1\u5668\u80fd\u529b\u3002<\/p>\n<p>\u591a\u4eba\u5171\u4eab\u8282\u70b9\u8fd8\u8981\u9650\u5236\u201c\u4efb\u52a1\u6570\u00d7\u7ebf\u7a0b\u6570\u201d\u3002\u56db\u4e2a 24 \u7ebf\u7a0b\u4efb\u52a1\u5e76\u4e0d\u7b49\u4e8e 96 \u6838\u5c31\u4e00\u5b9a\u5408\u7406\uff1a\u5b83\u4eec\u53ef\u80fd\u540c\u65f6\u62a2\u540c\u4e00\u5757 NVMe\u3001\u91cd\u590d\u89e3\u538b\u8f93\u5165\uff0c\u5e76\u4ea7\u751f\u5927\u4f53\u91cf\u9010 read \u8f93\u51fa\u3002\u82e5\u4e0b\u6e38\u53ea\u9700\u8981\u7269\u79cd\u62a5\u544a\uff0c\u53ef\u4ee5\u8bc4\u4f30\u662f\u5426\u4fdd\u7559\u5b8c\u6574 <code>--output<\/code>\uff1b\u82e5\u8981\u8ffd\u8e2a\u672a\u5206\u7c7b reads\u3001\u505a\u5bbf\u4e3b\u8fc7\u6ee4\u590d\u6838\u6216\u590d\u73b0\uff0c\u5219\u5e94\u63d0\u524d\u4e3a\u5206\u7c7b\u7ed3\u679c\u548c FASTQ \u526f\u672c\u9884\u7559\u5bb9\u91cf\u3002\u8d44\u6e90\u89c4\u5212\u8981\u56f4\u7ed5\u4ea4\u4ed8\u7269\uff0c\u800c\u4e0d\u662f\u53ea\u56f4\u7ed5\u547d\u4ee4\u80fd\u5426\u542f\u52a8\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">32 GB\u3001128 GB\u3001256 GB\uff0c\u5e94\u8be5\u600e\u6837\u9009\uff1f<\/h2>\n<p>\u5982\u679c\u53ea\u662f\u9a8c\u8bc1\u6d41\u7a0b\u6216\u505a\u8303\u56f4\u660e\u786e\u7684\u75c5\u6bd2\u4efb\u52a1\uff0c32 GB \u670d\u52a1\u5668\u901a\u5e38\u6709\u8db3\u591f\u7a7a\u95f4\u8fd0\u884c\u5c0f\u578b\u7d22\u5f15\uff1b\u82e5\u4f7f\u7528\u7ea6 14.9 GB \u7684 PlusPF-16\uff0c\u8fd8\u5e94\u9650\u5236\u5e76\u53d1\uff0c\u5e76\u89c2\u5bdf\u5b9e\u9645\u5cf0\u503c\u3002\u9762\u5bf9\u7ea6 91.8 GB \u7684 Standard \u7d22\u5f15\uff0c\u53ef\u4ee5\u4ece 128 GB \u5185\u5b58\u4e0e\u672c\u5730 NVMe \u8bc4\u4f30\u3002\u8981\u4f7f\u7528 221.8 GB \u7684 PlusPFP\uff0c256 GB \u53ea\u662f\u8f83\u7d27\u7684\u5de5\u7a0b\u8d77\u70b9\uff1b\u591a\u4eba\u540c\u65f6\u8dd1\u65f6\uff0c\u66f4\u5e94\u8003\u8651\u5185\u5b58\u6620\u5c04\u3001\u4efb\u52a1\u961f\u5217\u548c\u9875\u7f13\u5b58\u590d\u7528\uff0c\u800c\u4e0d\u662f\u76f4\u63a5\u628a\u5e76\u53d1\u6570\u8bbe\u6210 CPU \u6838\u6570\u3002<\/p>\n<p>\u8d44\u6e90\u6709\u9650\u65f6\uff0c\u53ef\u4ee5\u9009\u62e9\u5b98\u65b9\u516c\u5f00\u7684 8 GB\/16 GB capped \u6570\u636e\u5e93\uff0c\u6216\u5728\u81ea\u5efa\u5e93\u65f6\u4f7f\u7528 <code>--max-db-size<\/code> \u63a7\u5236 hash table \u4e0a\u9650\u3002\u5b98\u65b9\u624b\u518c\u8bf4\u660e\uff0c\u8d85\u8fc7\u4e0a\u9650\u65f6\u53c2\u8003\u5e93\u4f1a\u88ab\u4e0b\u91c7\u6837\u4ee5\u9002\u914d\u5927\u5c0f\u3002\u4ee3\u4ef7\u662f\u6570\u636e\u5e93\u8986\u76d6\u53d1\u751f\u53d8\u5316\uff0c\u56e0\u6b64\u5fc5\u987b\u628a\u201c\u80fd\u5728\u5c0f\u673a\u5668\u4e0a\u8fd0\u884c\u201d\u548c\u201c\u9002\u5408\u5f53\u524d\u79d1\u5b66\u95ee\u9898\u201d\u5206\u5f00\u5224\u65ad\uff0c\u5c24\u5176\u4e0d\u80fd\u628a\u53d7\u9650\u5e93\u7684\u9634\u6027\u7ed3\u679c\u89e3\u91ca\u6210\u6837\u672c\u4e2d\u4e00\u5b9a\u4e0d\u5b58\u5728\u8be5\u7269\u79cd\u3002<\/p>\n<p>\u81ea\u5efa\u6570\u636e\u5e93\u8fd8\u5305\u542b\u4e0b\u8f7d\u5206\u7c7b\u5b66\u6587\u4ef6\u3001\u6574\u7406\u53c2\u8003\u5e8f\u5217\u3001\u4f30\u7b97\u5bb9\u91cf\u548c\u586b\u5145 hash table \u7b49\u9636\u6bb5\u3002\u5b98\u65b9\u624b\u518c\u7ed9\u51fa\u7684\u5386\u53f2\u6807\u51c6\u5e93\u793a\u4f8b\u5728\u6784\u5efa\u65f6\u7ea6\u9700 100 GB \u78c1\u76d8\uff0c\u5e76\u63d0\u9192\u6784\u5efa\u8fc7\u7a0b\u6ca1\u6709\u5728\u5bb9\u91cf\u4f30\u7b97\u4e4b\u540e\u81ea\u52a8\u65ad\u70b9\u7eed\u8dd1\u3002\u4eca\u5929\u7684\u53c2\u8003\u96c6\u5408\u66f4\u5927\uff0c\u5b9e\u9645\u7a7a\u95f4\u5fc5\u987b\u4ee5\u5f53\u6b21 library\u3001taxonomy \u548c\u6700\u7ec8 <code>hash.k2d<\/code> \u4e3a\u51c6\uff1b\u6700\u597d\u628a\u4e0b\u8f7d\u533a\u3001\u6784\u5efa\u4e34\u65f6\u533a\u548c\u6b63\u5f0f\u53ea\u8bfb\u7d22\u5f15\u5206\u5f00\uff0c\u6784\u5efa\u5931\u8d25\u624d\u4e0d\u4f1a\u6c61\u67d3\u6b63\u5728\u4f7f\u7528\u7684\u6570\u636e\u5e93\u3002<\/p>\n<p>\u7ed3\u679c\u89e3\u91ca\u4e5f\u6709\u8fb9\u754c\u3002Kraken2 \u7684 confidence \u9608\u503c\u4e0d\u662f\u5177\u6709\u7edf\u4e00\u6982\u7387\u610f\u4e49\u7684\u201c\u51c6\u786e\u7387\u201d\uff0c\u4e0d\u540c\u6837\u672c\u590d\u6742\u5ea6\u548c\u6570\u636e\u5e93\u90fd\u4f1a\u6539\u53d8\u5206\u7c7b\u8868\u73b0\u3002\u9700\u8981\u4e30\u5ea6\u4f30\u8ba1\u65f6\uff0cBracken \u6587\u4ef6\u5fc5\u987b\u4e0e\u6570\u636e\u5e93\u53ca read length \u914d\u5957\uff1b\u53ea\u590d\u5236\u4e00\u4e2a\u65e7\u7684 Bracken \u6587\u4ef6\u5230\u65b0\u7d22\u5f15\u76ee\u5f55\uff0c\u867d\u7136\u6d41\u7a0b\u53ef\u80fd\u8fd0\u884c\uff0c\u5374\u5931\u53bb\u53ef\u6bd4\u6027\u3002\u670d\u52a1\u5668\u89e3\u51b3\u7684\u662f\u7a33\u5b9a\u8fd0\u884c\u548c\u53ef\u590d\u73b0\uff0c\u4e0d\u66ff\u4ee3\u9634\u6027\u5bf9\u7167\u3001\u6c61\u67d3\u8bc4\u4f30\u4e0e\u751f\u7269\u5b66\u9a8c\u8bc1\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u4e0a\u670d\u52a1\u5668\u524d\uff0c\u5148\u68c0\u67e5\u8fd9 5 \u9879<\/h2>\n<ol>\n<li><strong>\u770b index size\uff0c\u4e0d\u53ea\u770b\u538b\u7f29\u5305\u3002<\/strong> \u4e0b\u8f7d\u7a7a\u95f4\u3001\u89e3\u538b\u7a7a\u95f4\u3001\u8fd0\u884c\u5185\u5b58\u662f\u4e09\u7b14\u8d26\u3002<\/li>\n<li><strong>\u786e\u8ba4\u6570\u636e\u5e93\u8986\u76d6\u3002<\/strong> Standard\u3001PlusPF\u3001PlusPFP\u3001Viral \u548c capped \u7248\u672c\u56de\u7b54\u7684\u95ee\u9898\u4e0d\u540c\u3002<\/li>\n<li><strong>\u6570\u636e\u5e93\u653e\u672c\u5730 NVMe\u3002<\/strong> \u82e5\u53ea\u80fd\u7528 NFS\uff0c\u5148\u7528\u4ee3\u8868\u6837\u672c\u5b9e\u6d4b\u51b7\u7f13\u5b58\u4e0e\u70ed\u7f13\u5b58\u5dee\u5f02\u3002<\/li>\n<li><strong>\u56fa\u5b9a\u7248\u672c\u548c\u65e5\u671f\u3002<\/strong> \u4fdd\u5b58 Kraken2 \u7248\u672c\u3001\u6570\u636e\u5e93\u53d1\u5e03\u65e5\u671f\u3001MD5\u3001\u53c2\u6570\u4e0e Bracken \u6587\u4ef6\u3002<\/li>\n<li><strong>\u6309\u5e76\u53d1\u4f30\u7b97\u5cf0\u503c\u3002<\/strong> \u4e0d\u7528 memory mapping \u65f6\uff0c\u591a\u8fdb\u7a0b\u53ef\u80fd\u91cd\u590d\u88c5\u8f7d\uff1b\u4f7f\u7528\u540e\u4e5f\u8981\u7ed9\u9875\u7f13\u5b58\u548c\u7cfb\u7edf\u7559\u4f59\u91cf\u3002<\/li>\n<\/ol>\n<p>\u5982\u679c\u4f60\u7684 Kraken2 \u4efb\u52a1\u6b63\u5728 OOM\u3001\u6570\u636e\u5e93\u52a0\u8f7d\u6162\u3001\u591a\u4eba\u5e76\u53d1\u4e92\u76f8\u5f71\u54cd\uff0c\u6216\u4e0d\u77e5\u9053\u8be5\u9009 Standard\u3001PlusPF \u8fd8\u662f PlusPFP\uff0c\u53ef\u4ee5\u51c6\u5907\u6570\u636e\u5e93\u540d\u79f0\u4e0e\u65e5\u671f\u3001\u7d22\u5f15\u5927\u5c0f\u3001\u6837\u672c\u6570\u3001FASTQ \u603b\u91cf\u3001\u8ba1\u5212\u5e76\u53d1\u548c\u76ee\u6807\u8f93\u51fa\u3002\u4e91\u751f\u4fe1\u53ef\u5148\u5224\u65ad\u74f6\u9888\u5728\u5185\u5b58\u3001NVMe\u3001\u7f51\u7edc\u5b58\u50a8\u3001\u6570\u636e\u5e93\u8986\u76d6\u8fd8\u662f\u53c2\u6570\uff0c\u518d\u5206\u522b\u6c9f\u901a\u670d\u52a1\u5668\u79df\u8d41\u3001\u73af\u5883\u90e8\u7f72\u6216\u59d4\u6258\u5206\u6790\u7684\u9002\u7528\u8303\u56f4\u3002\u5177\u4f53\u901f\u5ea6\u548c\u5cf0\u503c\u4ee5\u4ee3\u8868\u6837\u672c\u5b9e\u6d4b\u4e3a\u51c6\u3002<\/p>\n<p><a href=\"https:\/\/work.weixin.qq.com\/kfid\/kfcb05492f29d4d6064\" data-ysx-event=\"contact_click\" style=\"display:inline-block;padding:13px 20px;border-radius:10px;background:#e36e2e;color:#fff;text-decoration:none;font-weight:800;\">\u8054\u7cfb\u4e91\u751f\u4fe1\uff0c\u54a8\u8be2 Kraken2 \u6570\u636e\u5e93\u4e0e\u670d\u52a1\u5668\u914d\u7f6e<\/a><\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u53c2\u8003\u8d44\u6599<\/h2>\n<ul>\n<li><a href=\"https:\/\/github.com\/DerrickWood\/kraken2\/wiki\/Manual\">Kraken2 \u5b98\u65b9\u624b\u518c\uff1a\u5185\u5b58\u3001\u78c1\u76d8\u3001memory mapping \u4e0e\u6570\u636e\u5e93\u6784\u5efa<\/a><\/li>\n<li><a href=\"https:\/\/benlangmead.github.io\/aws-indexes\/k2\">Langmead Lab AWS Index Zone\uff1aKraken2\/Bracken \u6570\u636e\u5e93\u5927\u5c0f\u4e0e\u65e5\u671f<\/a><\/li>\n<li><a href=\"https:\/\/github.com\/DerrickWood\/kraken2\/releases\">Kraken2 \u5b98\u65b9 Releases\uff1a\u5f53\u524d\u7248\u672c\u4e0e\u66f4\u65b0\u8bb0\u5f55<\/a><\/li>\n<\/ul>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Kraken2 \u7684\u8fd0\u884c\u8d44\u6e90\u7531\u6570\u636e\u5e93\u51b3\u5b9a\uff1a\u516c\u5f00\u7d22\u5f15\u4ece 0.6 GB \u5230 221.8 GB\uff0c\u76f8\u5dee\u7ea6 370 \u500d\u3002\u672c\u6587\u8bf4\u660e archive size\u3001index size\u3001\u5185\u5b58\u6620\u5c04\u3001\u672c\u5730 NVMe \u4e0e\u5e76\u53d1\u89c4\u5212\u3002<\/p>\n","protected":false},"author":1,"featured_media":66269,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[1,75],"tags":[401,405,404,383,403,275,402,352],"class_list":["post-66270","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-part","category-compute-environment","tag-kraken2","tag-nvme","tag-404","tag-383","tag-403","tag-275","tag-402","tag-352"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/daily-bio-blog-20261021-kraken2-memory-kraken2-memory-poster.png?fit=1080%2C1350&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66270","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=66270"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66270\/revisions"}],"predecessor-version":[{"id":66271,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66270\/revisions\/66271"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/66269"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=66270"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=66270"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=66270"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}