{"id":66287,"date":"2026-10-24T09:07:03","date_gmt":"2026-10-24T01:07:03","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66287"},"modified":"2026-10-24T09:07:03","modified_gmt":"2026-10-24T01:07:03","slug":"dorado-2-1-gpu-nvme-basecalling-planning","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/dorado-2-1-gpu-nvme-basecalling-planning\/","title":{"rendered":"8 GB \u663e\u5b58\u80fd\u8dd1 Dorado\uff0c\u4e3a\u4ec0\u4e48\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u4ecd\u7136\u9700\u8981\u670d\u52a1\u5668\uff1f\u74f6\u9888\u5e38\u5728 GPU \u4e4b\u5916"},"content":{"rendered":"<div id=\"articleContent\" style=\"max-width:860px;margin:0 auto;color:#1f2937;font-size:17px;line-height:1.85;\">\n<p>Oxford Nanopore \u539f\u59cb\u4fe1\u53f7\u4ece POD5 \u53d8\u6210\u53ef\u5206\u6790\u7684\u5e8f\u5217\uff0c\u8981\u7ecf\u8fc7 basecalling\uff1b\u5982\u679c\u8fd8\u8981\u540c\u65f6\u505a\u6bd4\u5bf9\u3001\u6761\u5f62\u7801\u62c6\u5206\u3001\u4fee\u9970\u78b1\u57fa\u8bc6\u522b\u548c BAM\/CRAM \u8f93\u51fa\uff0c\u8ba1\u7b97\u94fe\u4f1a\u8fc5\u901f\u53d8\u957f\u3002\u770b\u5230 Dorado \u5b98\u65b9\u5199\u7740\u201c\u591a\u6570 Pascal \u53ca\u66f4\u65b0\u67b6\u6784\u7684 NVIDIA GPU\u3001\u663e\u5b58\u4e0d\u4f4e\u4e8e 8 GB \u9884\u8ba1\u53ef\u8fd0\u884c\u201d\uff0c\u5f88\u591a\u4eba\u4f1a\u4ee5\u4e3a\u4e00\u5f20\u6d88\u8d39\u7ea7\u663e\u5361\u5c31\u8db3\u591f\u3002<\/p>\n<p>\u80fd\u542f\u52a8\u4e0d\u7b49\u4e8e\u80fd\u9ad8\u6548\u5b8c\u6210\u3002Dorado 2.1.2 \u5df2\u652f\u6301 v6.0 \u6a21\u578b\uff0c\u5e76\u6301\u7eed\u4f18\u5316 Blackwell GPU\u3001SUP \u77ed\u8bfb\u957f\u548c <code>smallvar<\/code>\u3002\u771f\u6b63\u51b3\u5b9a\u5468\u8f6c\u901f\u5ea6\u7684\uff0c\u662f\u6a21\u578b\u590d\u6742\u5ea6\u3001GPU \u6570\u91cf\u3001POD5 \u8bfb\u53d6\u3001\u8f93\u51fa\u683c\u5f0f\u3001\u4fee\u9970\u6a21\u578b\u4ee5\u53ca\u4e0b\u6e38\u5e76\u53d1\u5171\u540c\u5f62\u6210\u7684\u6d41\u6c34\u7ebf\u3002<\/p>\n<figure style=\"margin:28px 0;\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/daily-bio-blog-20261024-dorado-basecalling-dorado-basecalling-poster.png?ssl=1\" alt=\"Dorado \u7eb3\u7c73\u5b54\u6d4b\u5e8f GPU \u4e0e\u5b58\u50a8\u8d44\u6e90\u89c4\u5212\u6d77\u62a5\" style=\"display:block;width:100%;height:auto;border-radius:14px;\"><figcaption style=\"margin-top:8px;color:#64748b;font-size:14px;text-align:center;\">Dorado \u8d44\u6e90\u89c4\u5212\uff1a\u6a21\u578b\u3001GPU\u3001POD5 \u8bfb\u53d6\u3001\u8f93\u51fa\u683c\u5f0f\u4e0e NVMe \u5fc5\u987b\u4e00\u8d77\u8ba1\u7b97\u3002<\/figcaption><\/figure>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u5148\u770b\u56db\u4e2a\u6570\u5b57\uff0c\u518d\u8c08\u663e\u5361\u578b\u53f7<\/h2>\n<p>\u5b98\u65b9\u5e73\u53f0\u8bf4\u660e\u4e2d\uff0c\u672a\u88ab\u5e7f\u6cdb\u6d4b\u8bd5\u7684 Linux\/Windows NVIDIA \u8bbe\u5907\uff0c\u901a\u5e38\u8981\u6c42 <strong>Pascal \u53ca\u66f4\u65b0\u67b6\u6784\u3001\u81f3\u5c11 8 GB \u663e\u5b58<\/strong>\uff0c\u4f46 P100\/GP100 \u4f8b\u5916\uff1bApple Silicon \u5efa\u8bae\u81f3\u5c11 <strong>16 GB \u7edf\u4e00\u5185\u5b58<\/strong>\u3002\u8fd9\u53ea\u662f\u517c\u5bb9\u6027\u53c2\u8003\uff0c\u4e0d\u662f\u6240\u6709\u6a21\u578b\u4e0e\u6d41\u7a0b\u7684\u6027\u80fd\u4fdd\u8bc1\u3002<\/p>\n<p>Dorado 2.0 \u5c06 x86_64 \u5e73\u53f0\u6700\u4f4e CUDA \u63d0\u5347\u5230 <strong>12.8<\/strong>\uff1b2.1 \u7cfb\u5217\u8fdb\u4e00\u6b65\u4f18\u5316 v6.0 HAC \u5728 Blackwell \u4e0a\u7684\u6027\u80fd\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u5b98\u65b9 v1.4 \u7684\u5185\u90e8\u6d4b\u8bd5\u663e\u793a\uff1a\u5728\u540c\u65f6\u8f93\u51fa\u5168\u4e0a\u4e0b\u6587\u4fee\u9970\u4fe1\u606f\u548c move table \u65f6\uff0cCRAM \u6bd4 BAM \u6587\u4ef6\u7ea6\u5c0f <strong>35%<\/strong>\u3002\u663e\u5b58\u3001\u9a71\u52a8\u548c\u5b58\u50a8\u683c\u5f0f\u56e0\u6b64\u5fc5\u987b\u4e00\u8d77\u89c4\u5212\u3002<\/p>\n<div style=\"overflow-x:auto;margin:18px 0 26px;\">\n<table style=\"width:100%;border-collapse:collapse;min-width:720px;font-size:15px;\">\n<thead style=\"background:#0e5b68;color:#fff;\">\n<tr>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u51b3\u7b56\u70b9<\/th>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u4f1a\u6539\u53d8\u4ec0\u4e48<\/th>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u5b98\u65b9\u4f9d\u636e<\/th>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u8d44\u6e90\u5f71\u54cd<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\"><code>fast \/ hac \/ sup<\/code><\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u51c6\u786e\u6027\u4e0e\u901f\u5ea6\u53d6\u820d<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u5b98\u65b9\u63d0\u4f9b\u4e09\u7c7b\u6a21\u578b\uff0c\u67b6\u6784\u548c\u89c4\u6a21\u5dee\u5f02\u660e\u663e<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">SUP \u901a\u5e38\u66f4\u5403\u7b97\u529b\uff0c\u4e0d\u80fd\u53ea\u6cbf\u7528 FAST \u7684\u914d\u7f6e<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u5355\u5361\u6216\u591a\u5361<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u6837\u672c\u541e\u5410<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">Dorado \u652f\u6301 NVIDIA \u591a GPU\uff0c\u5e76\u53ef\u8fd1\u7ebf\u6027\u6269\u5c55<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">GPU \u589e\u52a0\u540e\uff0cCPU\u3001POD5 \u8bfb\u53d6\u548c\u5199\u76d8\u4e5f\u8981\u8ddf\u4e0a<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">BAM \u6216 CRAM<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u8f93\u51fa\u5bb9\u91cf\u4e0e\u517c\u5bb9<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u7279\u5b9a\u4fee\u9970+move table \u573a\u666f\u4e2d CRAM \u7ea6\u5c0f 35%<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">CRAM \u7701\u7a7a\u95f4\uff0c\u4f46\u8981\u6838\u5bf9\u540e\u7eed\u5de5\u5177\u517c\u5bb9\u6027<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u4fee\u9970\u78b1\u57fa\u6a21\u578b<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u6bcf\u6761 read \u7684\u989d\u5916\u63a8\u65ad<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">v6.0 \u63d0\u4f9b 5mC\/5hmC\u30014mC\/5mC\u30016mA \u7b49\u6a21\u578b<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u589e\u52a0 GPU \u8ba1\u7b97\u3001\u8f93\u51fa\u5b57\u6bb5\u548c\u5b58\u50a8\u538b\u529b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<h2 style=\"margin-top:34px;color:#12314a;\">8 GB \u4e3a\u4ec0\u4e48\u53ea\u80fd\u770b\u4f5c\u201c\u80fd\u5426\u5f00\u59cb\u201d\u7684\u95e8\u69db<\/h2>\n<p>Dorado \u4f1a\u81ea\u52a8\u9009\u62e9 batch size\uff0c\u4e5f\u5141\u8bb8\u624b\u52a8\u8c03\u6574\u3002\u66f4\u5927\u7684 batch \u5f80\u5f80\u80fd\u63d0\u9ad8\u73b0\u4ee3 GPU \u541e\u5410\uff0c\u4f46\u663e\u5b58\u4e0d\u8db3\u65f6\u4f1a\u76f4\u63a5\u5931\u8d25\uff1b\u66f4\u5c0f\u7684 batch \u867d\u7136\u66f4\u7a33\uff0c\u5374\u53ef\u80fd\u8ba9 GPU \u5403\u4e0d\u6ee1\u3002\u4e0d\u540c\u6a21\u578b\u3001read \u957f\u5ea6\u548c\u4fee\u9970\u4efb\u52a1\u7684\u663e\u5b58\u5360\u7528\u5e76\u4e0d\u76f8\u540c\uff0c\u56e0\u6b64\u4e0d\u80fd\u628a\u201c8 GB \u80fd\u8fd0\u884c\u201d\u7406\u89e3\u6210\u201c8 GB \u80fd\u7a33\u5b9a\u8dd1 SUP \u52a0\u591a\u79cd\u4fee\u9970\u201d\u3002<\/p>\n<p>\u66f4\u7a33\u7684\u505a\u6cd5\u662f\u5148\u7528\u4e00\u4e2a\u4ee3\u8868\u6027 POD5 \u5b50\u96c6\u8fdb\u884c\u57fa\u7ebf\u6d4b\u8bd5\uff0c\u8bb0\u5f55 reads\/s\u3001GPU \u5229\u7528\u7387\u3001\u5cf0\u503c\u663e\u5b58\u3001POD5 \u8bfb\u53d6\u901f\u5ea6\u548c BAM \u5199\u5165\u901f\u5ea6\u3002GPU \u5229\u7528\u7387\u4f4e\u65f6\uff0c\u4e0d\u8981\u7acb\u523b\u52a0\u5361\uff0c\u5148\u68c0\u67e5\u8f93\u5165\u662f\u5426\u4ecd\u662f FAST5 \u8f6c\u6362\u94fe\u3001POD5 \u662f\u5426\u4f4d\u4e8e\u6162\u901f\u7f51\u7edc\u76d8\u3001\u8f93\u51fa\u538b\u7f29\u548c\u6bd4\u5bf9\u662f\u5426\u5835\u4f4f\u6d41\u6c34\u7ebf\u3002<\/p>\n<p>\u57fa\u7ebf\u6d4b\u8bd5\u4e0d\u80fd\u53ea\u622a\u4e00\u6bb5\u77ed\u8bfb\u957f\u6587\u4ef6\u3002\u82e5\u6837\u672c\u4e2d\u65e2\u6709\u5927\u91cf\u77ed\u7247\u6bb5\uff0c\u53c8\u6709\u8d85\u957f reads\uff0c\u5e94\u5206\u522b\u89c2\u5bdf\u541e\u5410\u548c\u663e\u5b58\uff1bDorado 2.1.0 \u4e13\u95e8\u589e\u52a0\u4e86 v5.0 \u4ee5\u540e SUP \u6a21\u578b\u7684\u53ef\u53d8 chunk size\uff0c\u4ee5\u6539\u5584\u77ed reads \u6027\u80fd\uff0c\u8fd9\u4e5f\u8bf4\u660e read \u957f\u5ea6\u5206\u5e03\u4f1a\u5f71\u54cd\u5b9e\u9645\u901f\u5ea6\u3002\u6d4b\u8bd5\u65f6\u81f3\u5c11\u4fdd\u7559\u8f93\u5165 read \u6570\u3001\u6210\u529f\u8f93\u51fa read \u6570\u3001\u8fd0\u884c\u65f6\u95f4\u548c\u5931\u8d25\u65e5\u5fd7\uff0c\u907f\u514d\u628a\u201c\u8dd1\u5f97\u5feb\u201d\u5efa\u7acb\u5728\u6f0f\u8bfb\u6216\u63d0\u524d\u4e2d\u6b62\u4e0a\u3002<\/p>\n<p>\u8fd8\u5e94\u628a\u4e0b\u6e38\u9700\u6c42\u63d0\u524d\u5199\u6e05\u3002\u53ea\u9700\u8981\u6807\u51c6\u78b1\u57fa\u5e8f\u5217\uff0c\u4e0e\u540c\u65f6\u8c03\u7528 5mC\/5hmC\u30016mA\u3001\u8f93\u51fa move table \u5e76\u5728\u7ebf\u6bd4\u5bf9\uff0c\u8d44\u6e90\u8d1f\u62c5\u5b8c\u5168\u4e0d\u540c\u3002\u82e5\u540e\u7eed\u5de5\u5177\u53ea\u63a5\u53d7 BAM\uff0c\u5c31\u4e0d\u80fd\u53ea\u56e0 CRAM \u66f4\u7701\u7a7a\u95f4\u800c\u5207\u6362\uff1b\u82e5\u56e2\u961f\u957f\u671f\u5f52\u6863\uff0c\u624d\u503c\u5f97\u9a8c\u8bc1 CRAM \u89e3\u7801\u3001\u7d22\u5f15\u548c\u5171\u4eab\u8f6f\u4ef6\u94fe\u662f\u5426\u517c\u5bb9\u3002<\/p>\n<p>\u5b98\u65b9 troubleshooting \u660e\u786e\u6307\u51fa\uff0cPOD5 \u5728 I\/O \u53d7\u9650\u73af\u5883\u4e2d\u80fd\u63d0\u4f9b\u66f4\u597d\u7684\u8bfb\u53d6\u6027\u80fd\u3002\u82e5\u591a\u5f20 GPU \u540c\u65f6\u4ece\u5171\u4eab\u76ee\u5f55\u8bfb\u53d6\u5927\u91cf\u5c0f\u6587\u4ef6\uff0c\u7ebf\u6027\u6269\u5c55\u5f88\u5feb\u4f1a\u88ab\u5b58\u50a8\u541e\u5410\u6253\u65ad\u3002\u670d\u52a1\u5668\u7684\u610f\u4e49\u6b63\u5728\u4e8e\u628a GPU\u3001NVMe \u6682\u5b58\u548c\u8f93\u51fa\u5f52\u6863\u653e\u5728\u540c\u4e00\u5957\u53ef\u76d1\u63a7\u7684\u8d44\u6e90\u8ba1\u5212\u4e2d\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u4e00\u4e2a\u53ef\u590d\u73b0\u7684\u8fd0\u884c\u8d77\u70b9<\/h2>\n<p>\u5bf9 R10.4.1\u3001E8.2\u3001400 bps \u6570\u636e\uff0cv6.0 HAC \u662f\u5f53\u524d\u5b98\u65b9\u5217\u51fa\u7684 DNA \u6a21\u578b\u4e4b\u4e00\uff1b\u5177\u4f53\u6a21\u578b\u4ecd\u5fc5\u987b\u4e0e\u6d4b\u5e8f\u6761\u4ef6\u3001\u91c7\u6837\u9891\u7387\u548c\u4fee\u9970\u7c7b\u578b\u5339\u914d\u3002\u4e0b\u9762\u793a\u4f8b\u8ba9 Dorado \u81ea\u52a8\u9009\u62e9\u53ef\u7528 GPU\uff0c\u5e76\u628a BAM \u660e\u786e\u5199\u5165\u6587\u4ef6\uff1a<\/p>\n<pre style=\"overflow-x:auto;padding:18px;background:#102f47;color:#f8fafc;border-radius:12px;line-height:1.55;\"><code class=\"language-bash\">dorado basecaller \\\r\n  hac@v6.0.0 \\\r\n  \/data\/pod5\/ \\\r\n  --device cuda:all \\\r\n  --modified-bases 5mCG_5hmCG \\\r\n  &gt; sample.hac.v6.bam\r\n<\/code><\/pre>\n<p>\u6267\u884c\u524d\u5148\u7528 <code>dorado download --list<\/code> \u67e5\u770b\u5f53\u524d\u5b89\u88c5\u53ef\u7528\u6a21\u578b\u3002\u751f\u4ea7\u6d41\u7a0b\u5efa\u8bae\u56fa\u5b9a Dorado \u7248\u672c\u3001\u5b8c\u6574\u6a21\u578b\u540d\u3001POD5 \u6279\u6b21\u3001\u547d\u4ee4\u884c\u548c\u9a71\u52a8\u4fe1\u606f\uff1b\u53ea\u5199\u201c\u7528\u4e86 HAC\u201d\u65e0\u6cd5\u4fdd\u8bc1\u590d\u73b0\uff0c\u56e0\u4e3a <code>hac@latest<\/code> \u4f1a\u968f\u7740\u8f6f\u4ef6\u4e0e\u6a21\u578b\u66f4\u65b0\u800c\u53d8\u5316\u3002<\/p>\n<p>\u9700\u8981\u8fb9\u6d4b\u8fb9\u7b97\u65f6\uff0c\u8fd8\u8981\u5206\u6e05\u5b9e\u65f6 basecalling \u4e0e\u79bb\u7ebf\u8865\u7b97\u3002\u5b9e\u65f6\u4efb\u52a1\u66f4\u770b\u91cd\u7a33\u5b9a\u541e\u5410\uff0c\u79bb\u7ebf\u4efb\u52a1\u53ef\u4ee5\u901a\u8fc7\u961f\u5217\u628a\u591a\u4e2a flow cell \u5206\u914d\u5230\u4e0d\u540c GPU\u3002\u82e5\u4f7f\u7528 <code>cuda:all<\/code>\uff0c\u8981\u786e\u8ba4\u8f93\u51fa\u5199\u5165\u3001\u6bd4\u5bf9\u548c demux \u662f\u5426\u4e5f\u80fd\u627f\u53d7\u603b\u541e\u5410\uff1b\u5426\u5219 GPU \u589e\u52a0\u53ea\u4f1a\u628a\u74f6\u9888\u63a8\u5230\u78c1\u76d8\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u591a GPU \u5e76\u4e0d\u7b49\u4e8e\u6240\u6709\u6b65\u9aa4\u90fd\u7ebf\u6027\u52a0\u901f<\/h2>\n<p>Dorado basecalling \u652f\u6301\u591a GPU\uff0c\u4f46\u540c\u4e00\u9879\u76ee\u5e38\u5e38\u8fd8\u5305\u542b <code>aligner<\/code>\u3001<code>demux<\/code>\u3001<code>summary<\/code>\u3001\u4fee\u9970\u78b1\u57fa\u3001poly(A) \u4f30\u8ba1\u751a\u81f3 <code>smallvar<\/code>\u3002\u8fd9\u4e9b\u6b65\u9aa4\u5bf9 GPU\u3001CPU \u548c\u5185\u5b58\u7684\u4f7f\u7528\u6a21\u5f0f\u4e0d\u540c\u3002\u5c06\u6240\u6709\u529f\u80fd\u585e\u8fdb\u4e00\u4e2a\u957f\u547d\u4ee4\uff0c\u867d\u7136\u65b9\u4fbf\uff0c\u5374\u4e0d\u5229\u4e8e\u5b9a\u4f4d\u5931\u8d25\u548c\u590d\u8dd1\u3002<\/p>\n<p>\u5de5\u7a0b\u4e0a\u53ef\u4ee5\u628a\u4efb\u52a1\u62c6\u6210\u201c\u539f\u59cb POD5 \u6821\u9a8c\u2014basecalling\u2014\u6bd4\u5bf9\/\u62c6\u5206\u2014\u8d28\u63a7\u6c47\u603b\u2014\u5f52\u6863\u201d\uff0c\u6bcf\u4e00\u6b65\u4fdd\u7559\u6821\u9a8c\u503c\u548c\u65e5\u5fd7\u3002\u8282\u70b9\u672c\u5730 NVMe \u7528\u4e8e\u6b63\u5728\u5904\u7406\u7684\u6570\u636e\uff0c\u5b8c\u6210\u540e\u518d\u628a POD5\u3001BAM\/CRAM\u3001summary \u548c\u65e5\u5fd7\u8f6c\u5165\u5bb9\u91cf\u76d8\u3002\u8fd9\u6837\u67d0\u4e00\u6b65\u5931\u8d25\u65f6\u65e0\u9700\u4ece\u5934\u91cd\u590d\uff0c\u4e5f\u80fd\u907f\u514d\u4e2a\u4eba\u5de5\u4f5c\u7ad9\u957f\u671f\u88ab\u5360\u7528\u3002<\/p>\n<p>\u6279\u91cf\u9879\u76ee\u5e94\u6309\u5cf0\u503c\u800c\u4e0d\u662f\u5e73\u5747\u503c\u8ba1\u7b97\u7a7a\u95f4\uff1a\u539f\u59cb POD5\u3001\u4e34\u65f6 BAM\u3001\u6700\u7ec8 BAM\/CRAM\u3001\u7d22\u5f15\u3001\u62c6\u5206\u6587\u4ef6\u548c\u65e5\u5fd7\u53ef\u80fd\u540c\u65f6\u5b58\u5728\u3002\u82e5\u8fd8\u4fdd\u7559 move table\u3001\u4fee\u9970\u6807\u7b7e\u548c\u6bd4\u5bf9\u4fe1\u606f\uff0c\u5355\u7eaf\u6309\u201c\u6d4b\u5e8f\u539f\u59cb\u6570\u636e\u5927\u5c0f\u201d\u914d\u76d8\u901a\u5e38\u4f1a\u4f4e\u4f30\u3002<\/p>\n<p>\u961f\u5217\u8bbe\u8ba1\u4e5f\u8981\u907f\u514d\u4e00\u5f20\u5361\u88ab\u591a\u4e2a\u4efb\u52a1\u65e0\u5e8f\u4e89\u62a2\u3002\u5bf9\u5355\u5361\u8282\u70b9\uff0c\u53ef\u4ee5\u4e00\u6b21\u53ea\u8fd0\u884c\u4e00\u4e2a basecaller\uff1b\u5bf9\u591a\u5361\u8282\u70b9\uff0c\u53ef\u663e\u5f0f\u6307\u5b9a <code>cuda:0<\/code>\u3001<code>cuda:1<\/code>\uff0c\u6216\u5728\u786e\u8ba4\u5b58\u50a8\u80fd\u8ddf\u4e0a\u540e\u4f7f\u7528 <code>cuda:all<\/code>\u3002\u6bcf\u4e2a\u4efb\u52a1\u90fd\u8bbe\u7f6e\u72ec\u7acb\u8f93\u51fa\u76ee\u5f55\u3001\u4e34\u65f6\u76ee\u5f55\u548c\u65e5\u5fd7\uff0c\u5931\u8d25\u65f6\u8bb0\u5f55 Dorado \u7248\u672c\u3001\u6a21\u578b\u4e0b\u8f7d\u65f6\u95f4\u3001\u9a71\u52a8\u4e0e GPU \u72b6\u6001\u3002\u8fd9\u6837\u65e2\u80fd\u590d\u73b0\uff0c\u4e5f\u4fbf\u4e8e\u5224\u65ad\u4e0b\u4e00\u6b21\u5e94\u6269 GPU\u3001CPU \u8fd8\u662f\u78c1\u76d8\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u6a21\u578b\u66f4\u65b0\u5e26\u6765\u6027\u80fd\uff0c\u4e5f\u5e26\u6765\u7248\u672c\u8fb9\u754c<\/h2>\n<p>Dorado 2.1.2 \u4fee\u590d\u4e86 v6.0 HAC \u7684\u975e\u786e\u5b9a\u6027\u95ee\u9898\uff1b2.1.0 \u66f4\u65b0\u4e86 minimap2\uff0c\u5e76\u4f18\u5316 Blackwell \u4e0e\u77ed\u8bfb\u957f SUP \u6027\u80fd\u3002\u65e7\u6587\u7ae0\u4e2d\u7684\u901f\u5ea6\u6216 batch size \u4e0d\u5e94\u76f4\u63a5\u5f53\u6210\u65b0\u7248\u672c\u7ed3\u8bba\u3002\u5347\u7ea7\u524d\u5e94\u5148\u7528\u540c\u4e00\u6279 POD5 \u505a\u5c0f\u89c4\u6a21\u56de\u5f52\uff0c\u6bd4\u8f83 read \u6570\u3001Q score \u5206\u5e03\u3001\u957f\u5ea6\u5206\u5e03\u3001\u4fee\u9970\u8f93\u51fa\u548c\u4e0b\u6e38\u517c\u5bb9\u3002<\/p>\n<p>\u6b64\u5916\uff0cDorado \u5df2\u4e0d\u518d\u652f\u6301\u90e8\u5206\u65e7\u6d4b\u5e8f\u6761\u4ef6\uff0c\u4f8b\u5982 RNA002\u3001R9.4.1\u3001R10.3 \u548c R10.4\u3002\u82e5\u5386\u53f2\u6570\u636e\u4e0e\u5f53\u524d\u6a21\u578b\u4e0d\u5339\u914d\uff0c\u9700\u8981\u6309\u5b98\u65b9\u517c\u5bb9\u8bf4\u660e\u9009\u62e9\u65e7\u5de5\u5177\u6216\u5408\u9002\u7248\u672c\uff0c\u4e0d\u80fd\u4e3a\u4e86\u201c\u8dd1\u8d77\u6765\u201d\u5f3a\u884c\u5957\u7528\u65b0\u6a21\u578b\u3002<\/p>\n<p>\u5982\u679c\u4f60\u7684 Dorado \u4efb\u52a1\u51fa\u73b0 GPU \u5229\u7528\u7387\u4f4e\u3001\u591a\u5361\u6269\u5c55\u4e0d\u660e\u663e\u3001BAM \u5199\u76d8\u8ddf\u4e0d\u4e0a\u3001CUDA \u7248\u672c\u4e0d\u517c\u5bb9\uff0c\u6216\u4e0d\u786e\u5b9a HAC\/SUP\u3001\u4fee\u9970\u6a21\u578b\u3001NVMe \u548c\u5f52\u6863\u7a7a\u95f4\u600e\u6837\u7ec4\u5408\uff0c\u53ef\u4ee5\u51c6\u5907 Dorado \u7248\u672c\u3001\u6d4b\u5e8f\u6761\u4ef6\u3001\u6a21\u578b\u3001POD5 \u603b\u91cf\u3001GPU \u578b\u53f7\u3001\u8f93\u51fa\u8981\u6c42\u548c\u671f\u671b\u5b8c\u6210\u65f6\u95f4\u3002\u4e91\u751f\u4fe1\u4f1a\u5148\u5224\u65ad\u74f6\u9888\u5728\u6a21\u578b\u3001GPU\u3001I\/O \u8fd8\u662f\u6d41\u7a0b\u7f16\u6392\uff0c\u518d\u786e\u8ba4\u79df\u7528\u73af\u5883\u81ea\u884c\u8fd0\u884c\u3001\u73af\u5883\u90e8\u7f72\u4ea4\u63a5\u6216\u59d4\u6258\u5206\u6790\u7684\u8303\u56f4\u3002<\/p>\n<p><a href=\"https:\/\/work.weixin.qq.com\/kfid\/kfcb05492f29d4d6064\" data-ysx-event=\"contact_click\" style=\"display:inline-block;padding:13px 20px;border-radius:10px;background:#e36e2e;color:#fff;text-decoration:none;font-weight:800;\">\u8054\u7cfb\u4e91\u751f\u4fe1\uff0c\u54a8\u8be2 Dorado GPU \u4e0e\u957f\u8bfb\u957f\u5206\u6790\u65b9\u6848<\/a><\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u53c2\u8003\u8d44\u6599<\/h2>\n<ul>\n<li><a href=\"https:\/\/github.com\/nanoporetech\/dorado\/releases\">Dorado \u5b98\u65b9\u53d1\u5e03\u8bb0\u5f55<\/a><\/li>\n<li><a href=\"https:\/\/github.com\/nanoporetech\/dorado\">Dorado \u5b98\u65b9\u4ed3\u5e93\u4e0e\u5e73\u53f0\u8981\u6c42<\/a><\/li>\n<li><a href=\"https:\/\/software-docs.nanoporetech.com\/dorado\/latest\/models\/list\/\">Dorado \u5b98\u65b9\u6a21\u578b\u5217\u8868<\/a><\/li>\n<li><a href=\"https:\/\/software-docs.nanoporetech.com\/dorado\/latest\/models\/selection\/\">Dorado \u5b98\u65b9\u6a21\u578b\u9009\u62e9\u8bf4\u660e<\/a><\/li>\n<li><a href=\"https:\/\/software-docs.nanoporetech.com\/dorado\/latest\/troubleshooting\/troubleshooting\/\">Dorado \u5b98\u65b9\u6545\u969c\u6392\u67e5<\/a><\/li>\n<\/ul>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Dorado \u80fd\u5728 8GB \u663e\u5b58\u8bbe\u5907\u4e0a\u542f\u52a8\uff0c\u4e0d\u4ee3\u8868\u957f\u8bfb\u957f basecalling \u5df2\u7ecf\u914d\u7f6e\u5408\u7406\u3002\u672c\u6587\u62c6\u89e3 v6.0 \u6a21\u578b\u3001\u591a GPU\u3001POD5\u3001BAM\/CRAM \u4e0e NVMe \u89c4\u5212\u3002<\/p>\n","protected":false},"author":1,"featured_media":66286,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[1,75],"tags":[418,414,340,405,417,352,415,416],"class_list":["post-66287","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-part","category-compute-environment","tag-basecalling","tag-dorado","tag-gpu","tag-nvme","tag-pod5","tag-352","tag-415","tag-416"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/daily-bio-blog-20261024-dorado-basecalling-dorado-basecalling-poster.png?fit=1080%2C1350&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66287","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=66287"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66287\/revisions"}],"predecessor-version":[{"id":66288,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66287\/revisions\/66288"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/66286"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=66287"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=66287"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=66287"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}