{"id":66302,"date":"2026-10-27T09:08:22","date_gmt":"2026-10-27T01:08:22","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66302"},"modified":"2026-10-27T09:08:22","modified_gmt":"2026-10-27T01:08:22","slug":"cell-ranger-10-1-million-cells-resource-planning","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/cell-ranger-10-1-million-cells-resource-planning\/","title":{"rendered":"100 \u4e07\u7ec6\u80de\u8dd1 Cell Ranger 10.1\uff1a512 GB \u5185\u5b58\u4e4b\u5916\uff0c\u8fd8\u8981\u9632 1.61 TB \u5de5\u4f5c\u76d8\u5cf0\u503c"},"content":{"rendered":"<div id=\"articleContent\" style=\"max-width:860px;margin:0 auto;color:#1f2937;font-size:17px;line-height:1.85;\">\n<p>\u5355\u7ec6\u80de\u9879\u76ee\u505a\u5230\u51e0\u5341\u4e07\u3001\u4e0a\u767e\u4e07\u7ec6\u80de\u540e\uff0c\u6700\u5371\u9669\u7684\u8bef\u5224\u662f\uff1a\u8f6f\u4ef6\u6700\u4f4e\u8981\u6c42\u53ea\u6709 64 GB \u5185\u5b58\uff0c\u6240\u4ee5\u627e\u4e00\u53f0\u201c\u5927\u4e00\u70b9\u7684\u7535\u8111\u201d\u5c31\u80fd\u8dd1\u3002Cell Ranger v10.1 \u7684\u5b98\u65b9\u8d44\u6e90\u8868\u7ed9\u51fa\u4e86\u66f4\u6e05\u695a\u7684\u5206\u754c\u2014\u2014\u9488\u5bf9 <strong>100 \u4e07\u7ec6\u80de<\/strong>\u7684 Flex v2 GEX+AB+CRISPR \u4ee3\u8868\u6027\u6570\u636e\uff0c\u4e3a\u628a\u8fd0\u884c\u65f6\u95f4\u63a7\u5236\u5728\u4e24\u5929\u4ee5\u5185\uff0c\u8981\u6c42 <strong>32 \u6838\u3001512 GB RAM\u30012 TB \u53ef\u7528\u78c1\u76d8<\/strong>\u3002<\/p>\n<p>\u800c\u5b98\u65b9 EC2 \u5b9e\u6d4b\u4e2d\uff0c\u8fd9\u4e2a\u4efb\u52a1\u5899\u949f\u65f6\u95f4\u4e3a <strong>35.61 \u5c0f\u65f6<\/strong>\u3001\u6d88\u8017 <strong>561.57 core-hours<\/strong>\uff0c\u5de5\u4f5c\u76d8\u9ad8\u6c34\u4f4d\u8fbe\u5230 <strong>1611 GB<\/strong>\u3002\u6240\u4ee5\u767e\u4e07\u7ec6\u80de\u4efb\u52a1\u4e0d\u662f\u201c\u5185\u5b58\u591f\u5c31\u884c\u201d\uff0c\u800c\u662f CPU\u3001\u5185\u5b58\u3001NVMe\u3001\u4e34\u65f6\u6587\u4ef6\u3001\u5e76\u53d1\u548c\u5931\u8d25\u6062\u590d\u5fc5\u987b\u4e00\u8d77\u89c4\u5212\u3002<\/p>\n<figure style=\"margin:28px 0;\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/daily-bio-blog-20261027-cellranger-million-cellranger-million-poster.png?ssl=1\" alt=\"Cell Ranger 10.1 \u767e\u4e07\u7ec6\u80de\u8d44\u6e90\u4e0e\u670d\u52a1\u5668\u89c4\u5212\u6d77\u62a5\" style=\"display:block;width:100%;height:auto;border-radius:14px;\"><figcaption style=\"margin-top:8px;color:#64748b;font-size:14px;text-align:center;\">Cell Ranger \u767e\u4e07\u7ec6\u80de\u8d44\u6e90\u89c4\u5212\uff1a\u5185\u5b58\u3001\u5de5\u4f5c\u76d8\u3001NVMe\u3001\u5e76\u53d1\u4e0e\u5931\u8d25\u6062\u590d\u5fc5\u987b\u4e00\u8d77\u8ba1\u7b97\u3002<\/figcaption><\/figure>\n<h2 style=\"margin-top:34px;color:#12314a;\">64 GB \u662f\u8f6f\u4ef6\u95e8\u69db\uff0c\u4e0d\u662f\u767e\u4e07\u7ec6\u80de\u914d\u7f6e<\/h2>\n<p>Cell Ranger v10.1 \u7684\u57fa\u7840\u786c\u4ef6\u8981\u6c42\u662f 8 \u6838\u300164 GB \u5185\u5b58\u30011.5 TB \u53ef\u7528\u78c1\u76d8\uff0c\u63a8\u8350 64 \u6838\u3001128 GB \u5185\u5b58\uff1b\u4f46\u5b98\u65b9\u540c\u65f6\u5f3a\u8c03\uff0c\u5927\u6570\u636e\u96c6\u9700\u8981\u989d\u5916\u8d44\u6e90\u3002\u4e0b\u9762\u6570\u5b57\u6765\u81ea v10.1 \u7cfb\u7edf\u8981\u6c42\u4e0e AWS \u65f6\u95f4\u8bd5\u9a8c\uff0c\u6570\u636e\u7c7b\u578b\u3001\u8bfb\u6df1\u548c feature \u6570\u4e0d\u540c\uff0c\u4e0d\u80fd\u628a\u4efb\u610f\u9879\u76ee\u673a\u68b0\u5957\u5165\u540c\u4e00\u6863\u3002<\/p>\n<div style=\"overflow-x:auto;margin:18px 0 26px;\">\n<table style=\"width:100%;border-collapse:collapse;min-width:720px;font-size:15px;\">\n<thead style=\"background:#0e5b68;color:#fff;\">\n<tr>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u4ee3\u8868\u6027\u6570\u636e<\/th>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u4e3a\u4f4e\u4e8e2\u5929\u7ed9\u51fa\u7684\u8d44\u6e90<\/th>\n<th style=\"text-align: right;\">\u5b98\u65b9\u5899\u949f\u65f6\u95f4<\/th>\n<th style=\"text-align: right;\">\u5de5\u4f5c\u76d8\u9ad8\u6c34\u4f4d<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">2\u4e07\u7ec6\u80de\uff0c3\u2019\/5\u2019\/Flex GEX\uff0c1\u4e07\uff5e2\u4e07 reads\/cell<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">8\u6838\u300164 GB\u30010.5 TB<\/td>\n<td style=\"text-align: right;\">7.56\u5c0f\u65f6<\/td>\n<td style=\"text-align: right;\">126 GB<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">32\u4e07\u7ec6\u80de\uff0cFlex v1 GEX+AB\uff0c200 features<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">8\u6838\u300164 GB\u30011 TB<\/td>\n<td style=\"text-align: right;\">32.7\u5c0f\u65f6<\/td>\n<td style=\"text-align: right;\">621 GB<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">100\u4e07\u7ec6\u80de\uff0cFlex v2 GEX+AB+CRISPR\uff0c500 features<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">32\u6838\u3001512 GB\u30012 TB<\/td>\n<td style=\"text-align: right;\">35.61\u5c0f\u65f6<\/td>\n<td style=\"text-align: right;\">1611 GB<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>\u8fd9\u91cc\u7684 <strong>1611 GB \u662f\u78c1\u76d8\u9ad8\u6c34\u4f4d\uff0c\u4e0d\u662f\u5185\u5b58\u5cf0\u503c<\/strong>\u3002\u5b83\u89e3\u91ca\u4e86\u4e3a\u4ec0\u4e48\u4e00\u53f0\u6709 512 GB RAM\u3001\u4f46\u5de5\u4f5c\u76ee\u5f55\u53ea\u5269 1 TB \u7684\u8282\u70b9\u4ecd\u53ef\u80fd\u5931\u8d25\u3002\u8f93\u5165 FASTQ\u3001\u53c2\u8003\u57fa\u56e0\u7ec4\u3001pipestance\u3001\u4e2d\u95f4\u6587\u4ef6\u3001BAM\u3001\u77e9\u9635\u4e0e\u62a5\u544a\u4f1a\u5728\u8fd0\u884c\u9636\u6bb5\u540c\u65f6\u5b58\u5728\uff0c\u6700\u7ec8\u4ea4\u4ed8\u4f53\u79ef\u8fdc\u5c0f\u4e8e\u8fc7\u7a0b\u5cf0\u503c\u4e5f\u5f88\u6b63\u5e38\u3002<\/p>\n<p>\u5bb9\u91cf\u6d4b\u7b97\u5e94\u4ece\u201c\u4e00\u4e2a\u9879\u76ee\u201d\u62c6\u6210\u201c\u4e00\u4e2a\u4efb\u52a1\u5cf0\u503c\u201d\u3002\u4f8b\u5982 384 \u4e2a\u6837\u672c\u5e76\u4e0d\u7b49\u4e8e\u8981\u628a 384 \u4efd\u7ed3\u679c\u540c\u65f6\u5e38\u9a7b\u5185\u5b58\uff0c\u4f46\u6240\u6709 FASTQ\u3001\u89e3\u590d\u7528\u4fe1\u606f\u548c\u591a\u6587\u5e93\u4e2d\u95f4\u6587\u4ef6\u4f1a\u8fdb\u5165\u540c\u4e00\u4e2a\u8fd0\u884c\u94fe\u8def\u3002\u6700\u5c11\u8981\u5217\u51fa\u56db\u9879\uff1a\u8f93\u5165\u6570\u636e\u603b\u91cf\u3001\u9884\u8ba1\u7ec6\u80de\u4e0e reads\u3001\u662f\u5426\u5305\u542b\u6297\u4f53\u6216 CRISPR \u5e93\u3001\u662f\u5426\u4fdd\u7559 BAM\u3002\u518d\u6309\u201c\u8f93\u5165 + \u8fd0\u884c\u5cf0\u503c + \u6700\u7ec8\u7ed3\u679c + \u91cd\u8dd1\u7f13\u51b2\u201d\u5206\u533a\uff0c\u800c\u4e0d\u662f\u770b\u5230\u5b98\u65b9\u5199 2 TB \u5c31\u53ea\u51c6\u5907\u4e00\u4e2a\u6070\u597d 2 TB \u7684\u5206\u533a\u3002<\/p>\n<p>\u5982\u679c\u8282\u70b9\u8fd8\u627f\u62c5\u5176\u4ed6\u4efb\u52a1\uff0c\u5e94\u4ee5\u53ef\u72ec\u5360\u8d44\u6e90\u8ba1\u7b97\uff0c\u4e0d\u80fd\u628a\u673a\u5668\u6807\u79f0 512 GB \u5168\u90e8\u4ea4\u7ed9 Cell Ranger\u3002\u64cd\u4f5c\u7cfb\u7edf\u3001\u76d1\u63a7\u3001SSH \u4f1a\u8bdd\u3001\u6587\u4ef6\u7f13\u5b58\u548c\u5176\u4ed6\u7528\u6237\u90fd\u4f1a\u5360\u5185\u5b58\u3002\u78c1\u76d8\u4e5f\u8981\u770b\u8fd0\u884c\u65f6\u771f\u6b63\u53ef\u7528\u7a7a\u95f4\uff0c\u800c\u4e0d\u662f\u9635\u5217\u603b\u5bb9\u91cf\uff1b\u5feb\u7167\u3001\u56de\u6536\u7ad9\u3001\u914d\u989d\u548c\u7a00\u758f\u6587\u4ef6\u90fd\u53ef\u80fd\u8ba9\u201c\u770b\u8d77\u6765\u8fd8\u6709\u7a7a\u95f4\u201d\u4e0e\u5b9e\u9645\u53ef\u5199\u7a7a\u95f4\u4e0d\u4e00\u81f4\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u5148\u9650\u5236\u8d44\u6e90\uff0c\u518d\u63d0\u4ea4\u5168\u91cf\u4efb\u52a1<\/h2>\n<p>Cell Ranger \u672c\u5730\u6a21\u5f0f\u9ed8\u8ba4\u4f7f\u7528\u53ef\u7528\u5185\u5b58\u7684 90% \u548c\u5168\u90e8\u6838\u5fc3\u3002\u5728\u5171\u4eab\u670d\u52a1\u5668\u4e0a\u76f4\u63a5\u8fd0\u884c\uff0c\u53ef\u80fd\u6324\u5360\u5176\u4ed6\u7528\u6237\uff1b\u66f4\u7a33\u59a5\u7684\u505a\u6cd5\u662f\u663e\u5f0f\u9650\u5236\u8d44\u6e90\uff0c\u5e76\u7ed9\u64cd\u4f5c\u7cfb\u7edf\u548c\u6587\u4ef6\u7f13\u5b58\u7559\u4e0b\u4f59\u91cf\uff1a<\/p>\n<pre style=\"overflow-x:auto;padding:18px;background:#102f47;color:#f8fafc;border-radius:12px;line-height:1.55;\"><code class=\"language-bash\">cellranger multi \\\r\n  --id=flexv2_million \\\r\n  --csv=multi_config.csv \\\r\n  --localcores=32 \\\r\n  --localmem=480\r\n<\/code><\/pre>\n<p>\u4e0a\u4f8b\u9002\u7528\u4e8e\u603b\u5185\u5b58 512 GB \u8282\u70b9\u7684\u8d44\u6e90\u63a7\u5236\u793a\u610f\uff0c\u5e76\u4e0d\u662f\u6240\u6709\u767e\u4e07\u7ec6\u80de\u4efb\u52a1\u7684\u56fa\u5b9a\u7b54\u6848\u3002\u771f\u6b63\u63d0\u4ea4\u524d\uff0c\u5e94\u68c0\u67e5\u6837\u672c\u6570\u3001\u9884\u8ba1\u7ec6\u80de\u6570\u3001GEX reads\/cell\u3001Antibody\/CRISPR feature \u6570\u3001\u662f\u5426\u751f\u6210 BAM\u3001\u53c2\u8003\u7248\u672c\u548c\u5269\u4f59\u78c1\u76d8\u3002\u8f93\u5165\u89c4\u6a21\u4e0d\u540c\uff0c\u5cf0\u503c\u4f1a\u660e\u663e\u53d8\u5316\u3002<\/p>\n<p>Linux \u9650\u5236\u4e5f\u4e0d\u80fd\u5ffd\u7565\u3002\u5b98\u65b9\u5efa\u8bae\u7528\u6237\u53ef\u6253\u5f00\u6587\u4ef6\u6570\u81f3\u5c11 <strong>16k<\/strong>\uff0c\u7cfb\u7edf\u6700\u5927\u6587\u4ef6\u6570\u6309 Cell Ranger \u53ef\u7528\u5185\u5b58\u6bcf GB \u914d <strong>10k<\/strong>\uff0c\u7528\u6237\u8fdb\u7a0b\u6570\u6309\u6bcf\u4e2a\u53ef\u7528\u6838\u5fc3 <strong>64<\/strong>\u3002\u4efb\u52a1\u5728\u9ad8\u5e76\u53d1\u9636\u6bb5\u62a5 \u201ctoo many open files\u201d \u65f6\uff0c\u7ee7\u7eed\u52a0\u5185\u5b58\u901a\u5e38\u65e0\u6548\uff0c\u5e94\u5148\u68c0\u67e5 <code>ulimit -n<\/code>\u3001<code>ulimit -u<\/code> \u548c\u7cfb\u7edf\u7ea7\u9650\u5236\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u672c\u5730 Azimuth \u7701\u53bb\u4e0a\u4f20\uff0c\u4f46\u4e0d\u80fd\u66ff\u4ee3\u4eba\u5de5\u590d\u6838<\/h2>\n<p>v10.1 \u7684\u4e00\u4e2a\u91cd\u8981\u66f4\u65b0\uff0c\u662f\u4eba\u7c7b\u6837\u672c\u9ed8\u8ba4\u53ef\u8fd0\u884c\u672c\u5730 Pan-Human Azimuth \u6ce8\u91ca\uff0c\u4e0d\u9700\u8981 10x Cloud \u8d26\u6237\u3002\u5b98\u65b9\u8bf4\u660e\u8be5\u6a21\u578b\u57fa\u4e8e <strong>23 \u79cd\u7ec4\u7ec7\u3001380 \u79cd\u7ec6\u80de\u7c7b\u578b<\/strong>\u8bad\u7ec3\uff1b\u7b97\u6cd5\u5728\u5d4c\u5165\u7a7a\u95f4\u4e2d\u4e3a\u6bcf\u4e2a\u7ec6\u80de\u67e5\u627e <strong>500 \u4e2a\u8fd1\u90bb<\/strong>\uff0c\u53c2\u8003\u4f7f\u7528\u524d <strong>128 \u4e2a\u4e3b\u6210\u5206<\/strong>\u3002<\/p>\n<p>\u8fd9\u5bf9\u6570\u636e\u4e0d\u80fd\u79bb\u5f00\u672c\u5730\u73af\u5883\u7684\u56e2\u961f\u5f88\u6709\u4ef7\u503c\uff0c\u4f46\u6709\u4e09\u6761\u8fb9\u754c\u3002\u7b2c\u4e00\uff0c\u53ea\u652f\u6301\u4eba\u7c7b\u6837\u672c\uff1b\u7b2c\u4e8c\uff0c\u6a21\u578b\u4ecd\u662f beta\uff1b\u7b2c\u4e09\uff0c\u8bad\u7ec3\u65f6\u6392\u9664\u4e86\u764c\u75c7\u6570\u636e\u3002\u80bf\u7624\u3001\u5f3a\u6270\u52a8\u3001\u53d1\u80b2\u9636\u6bb5\u6216\u75be\u75c5\u7279\u5f02\u7ec6\u80de\u4e0d\u80fd\u628a\u81ea\u52a8\u6807\u7b7e\u5f53\u4f5c\u6700\u7ec8\u7ed3\u8bba\u3002\u8f93\u51fa\u4e2d\u4f4e\u4e8e <strong>100 UMI<\/strong> \u7684 barcode \u4f1a\u88ab\u6807\u4e3a Unassigned \u6216 Low UMI Barcode\uff0c\u4e5f\u5e94\u7ed3\u5408 QC \u5904\u7406\u3002<\/p>\n<p>\u672c\u5730\u6ce8\u91ca\u7ed3\u679c\u4f1a\u8fdb\u5165 <code>cell_types\/Azimuth\/<\/code>\uff0c\u5e76\u5728 <code>web_summary.html<\/code> \u4e2d\u663e\u793a\u3002\u4ea4\u4ed8\u65f6\u4e0d\u5e94\u53ea\u4fdd\u7559\u4e00\u5217 fine cell type\uff0c\u8fd8\u5e94\u540c\u65f6\u4fdd\u5b58 broad\u3001coarse\u3001fine \u4e09\u5c42\u6807\u7b7e\u3001\u53c2\u8003\u6a21\u578b\u4fe1\u606f\u548c\u6bcf\u7c7b\u7ec6\u80de\u6570\u91cf\u3002\u5bf9\u4e8e\u514d\u75ab\u7ec6\u80de\u4e9a\u7fa4\u3001\u80bf\u7624\u7ec6\u80de\u548c\u7a00\u6709\u7ec6\u80de\uff0c\u5efa\u8bae\u628a\u81ea\u52a8\u6807\u7b7e\u4e0e\u7ecf\u5178 marker\u3001\u53cc\u7ec6\u80de\u6307\u6807\u3001\u6837\u672c\u6765\u6e90\u548c\u6279\u6b21\u5206\u5e03\u4ea4\u53c9\u6838\u5bf9\uff1b\u5982\u679c\u4e0d\u540c\u5c42\u7ea7\u6807\u7b7e\u51b2\u7a81\uff0c\u5e94\u5148\u56de\u5230\u8868\u8fbe\u8bc1\u636e\uff0c\u800c\u4e0d\u662f\u4eba\u4e3a\u6311\u9009\u66f4\u201c\u597d\u770b\u201d\u7684\u540d\u79f0\u3002<\/p>\n<p>\u540c\u4e00\u6279\u6570\u636e\u5728\u5347\u7ea7 Cell Ranger \u6216\u53c2\u8003\u6a21\u578b\u540e\u91cd\u65b0\u6ce8\u91ca\uff0c\u6807\u7b7e\u6bd4\u4f8b\u53ef\u80fd\u6539\u53d8\u3002\u56e0\u6b64\uff0c\u6d41\u7a0b\u8bb0\u5f55\u4e2d\u8981\u540c\u65f6\u5199\u660e Cell Ranger \u7248\u672c\u3001\u8f6c\u5f55\u7ec4\u53c2\u8003\u3001Azimuth \u6a21\u578b\u7248\u672c\u548c\u662f\u5426\u542f\u7528\u4e91\u7aef\u6a21\u578b\u3002\u53ea\u6709\u56fa\u5b9a\u8fd9\u4e9b\u4fe1\u606f\uff0c\u540e\u7eed\u8865\u6837\u3001\u8fd4\u4fee\u6216\u4e0d\u540c\u670d\u52a1\u5668\u91cd\u8dd1\u65f6\uff0c\u5dee\u5f02\u624d\u6709\u673a\u4f1a\u88ab\u89e3\u91ca\u3002<\/p>\n<p>\u5982\u679c\u9009\u62e9 10x \u7684\u4e91\u7aef\u6ce8\u91ca\u6a21\u578b\uff0c\u6570\u636e\u4f1a\u4f20\u8f93\u5230 10x Cloud\uff0c\u5e76\u53d7\u76f8\u5e94\u6761\u6b3e\u548c\u533a\u57df\u9650\u5236\u7ea6\u675f\u3002\u9879\u76ee\u6709\u9690\u79c1\u3001\u533b\u9662\u6570\u636e\u6216\u4f26\u7406\u9650\u5236\u65f6\uff0c\u5e94\u5728\u5206\u6790\u524d\u786e\u5b9a\u4f7f\u7528\u672c\u5730 Azimuth\u3001\u5173\u95ed\u6ce8\u91ca\uff0c\u8fd8\u662f\u91c7\u7528\u56e2\u961f\u8ba4\u53ef\u7684\u5176\u4ed6\u672c\u5730\u53c2\u8003\u6620\u5c04\u6d41\u7a0b\uff0c\u800c\u4e0d\u662f\u8dd1\u5230\u4e00\u534a\u624d\u5904\u7406\u5408\u89c4\u95ee\u9898\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u767e\u4e07\u7ec6\u80de\u4efb\u52a1\u600e\u6837\u505a\u5f97\u66f4\u7a33<\/h2>\n<p>\u7b2c\u4e00\uff0c\u5148\u7528\u4e00\u90e8\u5206 FASTQ \u6216\u8f83\u5c0f\u6279\u6b21\u5b8c\u6210\u9884\u6f14\uff0c\u8bb0\u5f55\u78c1\u76d8\u589e\u957f\u66f2\u7ebf\u3001\u5cf0\u503c\u5185\u5b58\u3001CPU \u5229\u7528\u7387\u548c\u5404\u9636\u6bb5\u8017\u65f6\u3002\u82e5 CPU \u5229\u7528\u7387\u957f\u671f\u4f4e\u800c\u78c1\u76d8\u961f\u5217\u5f88\u9ad8\uff0c\u74f6\u9888\u66f4\u53ef\u80fd\u5728\u5171\u4eab\u5b58\u50a8\uff1b\u6b64\u65f6\u589e\u52a0\u6838\u5fc3\u53cd\u800c\u4f1a\u653e\u5927 I\/O \u7ade\u4e89\u3002<\/p>\n<p>\u9884\u6f14\u6837\u672c\u4e0d\u80fd\u53ea\u9009\u6570\u636e\u91cf\u6700\u5c0f\u7684\u4e00\u4efd\u3002\u66f4\u5408\u7406\u7684\u662f\u9009\u62e9 reads \u8f83\u6df1\u3001feature \u8f83\u591a\u3001\u7ec6\u80de\u56de\u6536\u91cf\u8f83\u9ad8\u7684\u4ee3\u8868\u6837\u672c\uff0c\u5e76\u68c0\u67e5\u591a\u6587\u5e93\u914d\u5bf9\u548c sample barcode \u914d\u7f6e\u3002\u8fd9\u6837\u6d4b\u5f97\u7684\u5cf0\u503c\u66f4\u63a5\u8fd1\u6b63\u5f0f\u4efb\u52a1\uff1b\u82e5\u9884\u6f14\u53ea\u8986\u76d6\u6700\u7b80\u5355\u6837\u672c\uff0c\u653e\u5927\u5230\u5168\u91cf\u65f6\u4ecd\u53ef\u80fd\u5728\u805a\u5408\u3001\u964d\u7ef4\u6216\u6ce8\u91ca\u9636\u6bb5\u51fa\u73b0\u65b0\u7684\u8d44\u6e90\u5cf0\u503c\u3002<\/p>\n<p>\u7b2c\u4e8c\uff0c\u628a\u5de5\u4f5c\u76ee\u5f55\u653e\u5728\u672c\u5730 NVMe\/SSD\uff0c\u5b8c\u6210\u540e\u518d\u628a\u7ed3\u679c\u3001\u65e5\u5fd7\u548c\u5fc5\u8981\u4e2d\u95f4\u6587\u4ef6\u8fc1\u79fb\u5230\u5171\u4eab\u5b58\u50a8\u3002\u5b98\u65b9\u4e5f\u660e\u786e\u63a8\u8350 NVMe \u6216 SSD\uff0c\u800c\u4e0d\u662f\u673a\u68b0\u786c\u76d8\u3002\u81f3\u5c11\u4fdd\u7559 pipestance\u3001\u5b8c\u6574\u547d\u4ee4\u3001<code>multi_config.csv<\/code>\u3001\u8f6f\u4ef6\u4e0e\u53c2\u8003\u7248\u672c\uff0c\u4fbf\u4e8e\u4ece\u5931\u8d25\u9636\u6bb5\u6062\u590d\u548c\u590d\u73b0\u3002<\/p>\n<p>\u7b2c\u4e09\uff0c\u767e\u4e07\u7ec6\u80de\u5b9e\u9a8c\u5f80\u5f80\u5305\u542b\u6570\u5341\u5230\u6570\u767e\u4e2a\u6837\u672c\u3002\u4e0d\u8981\u540c\u65f6\u542f\u52a8\u591a\u4e2a 512 GB \u7ea7\u4efb\u52a1\uff1b\u5e94\u6309\u8282\u70b9\u771f\u5b9e\u5185\u5b58\u4e0e\u78c1\u76d8\u5cf0\u503c\u9650\u5236\u5e76\u53d1\u3002Cell Ranger \u652f\u6301 Slurm \u96c6\u7fa4\u6a21\u5f0f\uff0c\u5b98\u65b9\u4e5f\u5efa\u8bae\u5927\u578b\u6570\u636e\u91c7\u7528\u96c6\u7fa4\uff0c\u4f46\u5171\u4eab\u6587\u4ef6\u7cfb\u7edf\u4e0d\u7a33\u5b9a\u65f6\uff0c\u5355\u4e2a\u9ad8\u5185\u5b58\u8282\u70b9\u53ef\u80fd\u66f4\u5bb9\u6613\u6392\u9519\u3002<\/p>\n<p>\u7b2c\u56db\uff0c\u6d41\u7a0b\u8dd1\u5b8c\u540e\u4ecd\u8981\u68c0\u67e5\u6bcf\u6837\u672c reads\u3001\u6709\u6548 barcode\u3001UMI\u3001mapping\u3001\u6297\u4f53\u6216CRISPR feature\u3001\u6279\u6b21\u548c\u7ec6\u80de\u7c7b\u578b\u5206\u5e03\u3002Cell Ranger 10.1 \u7684\u6c47\u603b QC \u80fd\u51cf\u5c11\u9010\u4e2a\u6253\u5f00\u62a5\u544a\u7684\u5de5\u4f5c\u91cf\uff0c\u5374\u4e0d\u80fd\u66ff\u4ee3\u5bf9\u5f02\u5e38\u6837\u672c\u548c\u81ea\u52a8\u6ce8\u91ca\u7684\u9010\u9879\u6838\u67e5\u3002<\/p>\n<p>\u5982\u679c\u4f60\u7684\u4efb\u52a1\u5361\u5728 128\/512 GB \u5185\u5b58\u9009\u62e9\u30012 TB \u5de5\u4f5c\u76d8\u3001Flex v2 \u591a\u6837\u672c\u6392\u961f\u3001\u672c\u5730 Azimuth\u3001Slurm \u6216\u5931\u8d25\u6062\u590d\uff0c\u53ef\u4ee5\u51c6\u5907 Cell Ranger \u7248\u672c\u3001\u6587\u5e93\u7c7b\u578b\u3001\u6837\u672c\u4e0e\u9884\u8ba1\u7ec6\u80de\u6570\u3001reads\/cell\u3001feature \u6570\u3001\u73b0\u6709\u670d\u52a1\u5668\u548c\u671f\u671b\u65f6\u95f4\u3002\u4e91\u751f\u4fe1\u53ef\u5148\u505a\u8d44\u6e90\u6d4b\u7b97\u4e0e\u5c0f\u89c4\u6a21\u57fa\u7ebf\uff0c\u518d\u786e\u8ba4\u79df\u7528\u73af\u5883\u81ea\u884c\u8fd0\u884c\u3001\u670d\u52a1\u5668\u73af\u5883\u90e8\u7f72\u4ea4\u63a5\u6216\u59d4\u6258\u5206\u6790\u7684\u8303\u56f4\u3002<\/p>\n<p><a href=\"https:\/\/work.weixin.qq.com\/kfid\/kfcb05492f29d4d6064\" data-ysx-event=\"contact_click\" style=\"display:inline-block;padding:13px 20px;border-radius:10px;background:#e36e2e;color:#fff;text-decoration:none;font-weight:800;\">\u8054\u7cfb\u4e91\u751f\u4fe1\uff0c\u54a8\u8be2 Cell Ranger \u767e\u4e07\u7ec6\u80de\u670d\u52a1\u5668\u4e0e\u5206\u6790\u65b9\u6848<\/a><\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u53c2\u8003\u8d44\u6599<\/h2>\n<ul>\n<li><a href=\"https:\/\/www.10xgenomics.com\/support\/software\/cell-ranger\/downloads\/cr-system-requirements\">Cell Ranger v10.1 \u5b98\u65b9\u7cfb\u7edf\u8981\u6c42\u4e0e\u6027\u80fd\u8868<\/a><\/li>\n<li><a href=\"https:\/\/www.10xgenomics.com\/support\/software\/cell-ranger\/latest\/release-notes\/cr-release-notes\">Cell Ranger v10.1 \u5b98\u65b9\u53d1\u5e03\u8bf4\u660e<\/a><\/li>\n<li><a href=\"https:\/\/www.10xgenomics.com\/support\/software\/cell-ranger\/latest\/algorithms-overview\/cr-cell-annotation-algorithm\">Cell Ranger \u5b98\u65b9\u7ec6\u80de\u6ce8\u91ca\u7b97\u6cd5\u8bf4\u660e<\/a><\/li>\n<li><a href=\"https:\/\/www.10xgenomics.com\/support\/software\/cell-ranger\/latest\/analysis\/running-pipelines\/cr-gex-count\">Cell Ranger \u5b98\u65b9 count \u8fd0\u884c\u53c2\u6570<\/a><\/li>\n<\/ul>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Cell Ranger 10.1\u5904\u7406100\u4e07\u7ec6\u80de\u4ee3\u8868\u6027\u6570\u636e\uff0c\u5b98\u65b9\u7ed9\u51fa512GB\u5185\u5b58\u30012TB\u53ef\u7528\u78c1\u76d8\uff0c\u5b9e\u6d4b\u5de5\u4f5c\u76d8\u9ad8\u6c34\u4f4d1611GB\u3002\u672c\u6587\u62c6\u89e3\u8d44\u6e90\u3001\u5e76\u53d1\u3001\u672c\u5730Azimuth\u4e0e\u7a33\u5b9a\u8fd0\u884c\u65b9\u6cd5\u3002<\/p>\n","protected":false},"author":1,"featured_media":66301,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[1,75],"tags":[430,427,428,405,79,352,429,431],"class_list":["post-66302","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-part","category-compute-environment","tag-azimuth","tag-cell-ranger-10-1","tag-flex-v2","tag-nvme","tag-79","tag-352","tag-429","tag-431"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/daily-bio-blog-20261027-cellranger-million-cellranger-million-poster.png?fit=1080%2C1350&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66302","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=66302"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66302\/revisions"}],"predecessor-version":[{"id":66303,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66302\/revisions\/66303"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/66301"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=66302"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=66302"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=66302"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}