{"id":66313,"date":"2026-10-29T09:08:48","date_gmt":"2026-10-29T01:08:48","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66313"},"modified":"2026-10-29T09:08:48","modified_gmt":"2026-10-29T01:08:48","slug":"nanomdbg-400gbp-metagenome-resource-planning","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/nanomdbg-400gbp-metagenome-resource-planning\/","title":{"rendered":"400 Gbp \u571f\u58e4\u5b8f\u57fa\u56e0\u7ec4\uff1ananoMDBG 6 \u5929\u3001125 GB \u5185\u5b58\uff0c\u9009\u9519\u7b97\u6cd5\u53ef\u80fd\u8981 753 GB"},"content":{"rendered":"<div id=\"articleContent\" style=\"max-width:860px;margin:0 auto;color:#1f2937;font-size:17px;line-height:1.85;\">\n<p>\u957f\u8bfb\u957f\u5b8f\u57fa\u56e0\u7ec4\u6700\u5bb9\u6613\u51fa\u73b0\u7684\u8bef\u5224\uff0c\u662f\u628a\u201c\u6570\u636e\u91cf\u5927\u201d\u76f4\u63a5\u7b49\u540c\u4e8e\u201c\u4e70\u66f4\u5927\u7684\u670d\u52a1\u5668\u201d\u30022026 \u5e74 Nature Communications \u53d1\u8868\u7684 nanoMDBG \u8bc1\u660e\uff0c\u7b97\u6cd5\u9009\u62e9\u540c\u6837\u4f1a\u628a\u8d44\u6e90\u5dee\u8ddd\u62c9\u5f00\uff1a\u5728 <strong>400 Gbp<\/strong> \u7684 Nanopore \u571f\u58e4\u6570\u636e\u4e0a\uff0cnanoMDBG \u7528 <strong>125 GB \u5185\u5b58\u30016 \u5929<\/strong>\u5b8c\u6210\u7ec4\u88c5\uff1bmetaFlye \u5728\u540c\u4e00\u4efb\u52a1\u4e2d\u9700\u8981 <strong>753 GB \u5185\u5b58\u300128 \u5929<\/strong>\u3002<\/p>\n<p>\u8fd9\u5e76\u4e0d\u662f\u8bf4 125 GB \u53ef\u4ee5\u968f\u4fbf\u627e\u4e00\u53f0\u7535\u8111\u8dd1\u3002400 Gbp \u539f\u59cb reads\u3001\u7ea0\u9519\u4e2d\u95f4\u6587\u4ef6\u3001\u7ec4\u88c5\u56fe\u3001reads \u56de\u8d34 BAM \u548c\u540e\u7eed\u5206\u7bb1\u4ecd\u4f1a\u5360\u7528\u5927\u91cf CPU\u3001\u5185\u5b58\u4e0e\u78c1\u76d8\u3002\u771f\u6b63\u7a33\u59a5\u7684\u505a\u6cd5\uff0c\u662f\u5148\u6309\u6d4b\u5e8f\u7c7b\u578b\u548c\u6570\u636e\u590d\u6742\u5ea6\u9009 assembler\uff0c\u518d\u505a\u5c0f\u89c4\u6a21\u57fa\u7ebf\u548c\u8d28\u91cf\u9a8c\u8bc1\uff0c\u800c\u4e0d\u662f\u53ea\u6bd4\u8f83\u8c01\u7684 N50 \u66f4\u9ad8\u3002<\/p>\n<figure style=\"margin:28px 0;\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/daily-bio-blog-20261029-nanomdbg-metagenome-nanomdbg-poster.png?ssl=1\" alt=\"nanoMDBG \u957f\u8bfb\u957f\u5b8f\u57fa\u56e0\u7ec4\u7ec4\u88c5\u8d44\u6e90\u89c4\u5212\u6d77\u62a5\" style=\"display:block;width:100%;height:auto;border-radius:14px;\"><figcaption style=\"margin-top:8px;color:#64748b;font-size:14px;text-align:center;\">\u957f\u8bfb\u957f\u5b8f\u57fa\u56e0\u7ec4\u8d44\u6e90\u89c4\u5212\uff1a\u5148\u6309 chemistry \u548c\u6570\u636e\u89c4\u6a21\u9009 assembler\uff0c\u518d\u914d\u7f6e CPU\u3001\u5185\u5b58\u3001\u5de5\u4f5c\u76d8\u4e0e\u56de\u8d34\u8d28\u63a7\u3002<\/figcaption><\/figure>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u540c\u4e00\u6279 ONT reads\uff0c\u8d44\u6e90\u5dee\u8ddd\u53ef\u4ee5\u8d85\u8fc7 6 \u500d<\/h2>\n<p>nanoMDBG \u5df2\u96c6\u6210\u5230 metaMDBG\uff0c\u4e3b\u8981\u9762\u5411 Nanopore R10 simplex \u957f\u8bfb\u957f\u3002\u8bba\u6587\u8fd8\u6bd4\u8f83\u4e86 50 Gbp \u4eba\u80a0\u9053\u6570\u636e\u548c\u66f4\u590d\u6742\u7684\u571f\u58e4\u6570\u636e\u3002\u4e0b\u8868\u662f\u8bba\u6587\u7ed9\u51fa\u7684\u5b9e\u9645\u57fa\u51c6\uff0c\u4e0d\u662f\u5bf9\u4efb\u610f\u6837\u672c\u7684\u8017\u65f6\u4fdd\u8bc1\u3002<\/p>\n<div style=\"overflow-x:auto;margin:18px 0 26px;\">\n<table style=\"width:100%;border-collapse:collapse;min-width:720px;font-size:15px;\">\n<thead style=\"background:#0e5b68;color:#fff;\">\n<tr>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u6570\u636e\u4e0e\u5de5\u5177<\/th>\n<th style=\"text-align: right;\">\u8fd0\u884c\u65f6\u95f4<\/th>\n<th style=\"text-align: right;\">\u5cf0\u503c\u5185\u5b58<\/th>\n<th style=\"padding:12px;border:1px solid #d6e2e5;text-align:left;\">\u8d44\u6e90\u5224\u65ad<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">50 Gbp \u4eba\u80a0\u9053\uff0cnanoMDBG<\/td>\n<td style=\"text-align: right;\">5 \u5c0f\u65f6<\/td>\n<td style=\"text-align: right;\">17 GB<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u53ef\u5148\u5728\u4e2d\u7b49\u5185\u5b58\u8282\u70b9\u9a8c\u8bc1\u6d41\u7a0b<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">50 Gbp \u4eba\u80a0\u9053\uff0cmetaFlye<\/td>\n<td style=\"text-align: right;\">8 \u5c0f\u65f6<\/td>\n<td style=\"text-align: right;\">115 GB<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u5185\u5b58\u9700\u6c42\u7ea6\u9ad8 6.8 \u500d<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">400 Gbp \u571f\u58e4\uff0cnanoMDBG<\/td>\n<td style=\"text-align: right;\">6 \u5929<\/td>\n<td style=\"text-align: right;\">125 GB<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u9700\u957f\u65f6\u7a33\u5b9a\u8282\u70b9\u4e0e\u6301\u4e45\u5316\u5de5\u4f5c\u76d8<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">400 Gbp \u571f\u58e4\uff0cmetaFlye<\/td>\n<td style=\"text-align: right;\">28 \u5929<\/td>\n<td style=\"text-align: right;\">753 GB<\/td>\n<td style=\"padding:11px;border:1px solid #d6e2e5;vertical-align:top;\">\u5df2\u8fdb\u5165\u8d85\u5927\u5185\u5b58\u670d\u52a1\u5668\u8303\u56f4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>\u5728 400 Gbp \u571f\u58e4\u6570\u636e\u4e2d\uff0cnanoMDBG \u7684 correction \u9636\u6bb5\u7528 32 \u6838\u8fd0\u884c\u7ea6 <strong>16 \u5c0f\u65f6<\/strong>\u3001\u5cf0\u503c <strong>74 GB<\/strong>\uff0c\u53ea\u5360\u603b\u7ec4\u88c5\u65f6\u95f4\u7ea6 10%\u3002\u8fd9\u8bf4\u660e\u540e\u7eed\u56fe\u6784\u5efa\u548c\u89e3\u6790\u4ecd\u662f\u957f\u4efb\u52a1\uff0c\u4e0d\u80fd\u770b\u5230\u7ea0\u9519\u7ed3\u675f\u5c31\u5224\u65ad\u201c\u5feb\u8dd1\u5b8c\u4e86\u201d\u3002\u8c03\u5ea6\u65f6\u5e94\u4e3a\u6574\u4e2a assembly \u9884\u7559\u8fde\u7eed\u65f6\u95f4\uff0c\u5e76\u4fdd\u7559\u53ef\u6062\u590d\u8f93\u51fa\u3002<\/p>\n<p>\u8d44\u6e90\u9884\u4f30\u4e0d\u80fd\u53ea\u6309 FASTQ \u6587\u4ef6\u5927\u5c0f\u4e58\u500d\u6570\u3002gzip \u538b\u7f29\u7387\u4f1a\u53d7\u78b1\u57fa\u8d28\u91cf\u548c\u5e8f\u5217\u590d\u6742\u5ea6\u5f71\u54cd\uff0c\u540c\u6837 100 GB \u538b\u7f29\u6587\u4ef6\u53ef\u80fd\u5bf9\u5e94\u4e0d\u540c\u603b\u78b1\u57fa\u91cf\uff1b\u571f\u58e4\u3001\u80a0\u9053\u548c mock community \u7684\u7269\u79cd\u591a\u6837\u6027\u3001\u4e30\u5ea6\u8de8\u5ea6\u548c\u91cd\u590d\u7ed3\u6784\u4e5f\u4e0d\u540c\u3002\u54a8\u8be2\u6216\u6392\u961f\u65f6\u5e94\u63d0\u4f9b\u603b reads\u3001\u603b bases\u3001read N50\u3001\u8d28\u91cf\u5206\u5e03\u548c\u6837\u672c\u7c7b\u578b\uff0c\u800c\u4e0d\u662f\u53ea\u62a5\u201c\u4e00\u4e2a Nanopore \u6837\u672c\u201d\u3002<\/p>\n<p>\u6b63\u5f0f\u5168\u91cf\u7ec4\u88c5\u524d\uff0c\u53ef\u4ee5\u5bf9\u4ee3\u8868\u6027 reads \u5b50\u96c6\u505a\u73af\u5883\u9a8c\u6536\uff0c\u786e\u8ba4\u547d\u4ee4\u3001\u8f93\u51fa\u76ee\u5f55\u3001\u7248\u672c\u548c\u76d1\u63a7\u90fd\u6b63\u5e38\u3002\u4f46\u5b50\u96c6\u8fd0\u884c\u65f6\u95f4\u4e0e\u5185\u5b58\u4e0d\u80fd\u7b80\u5355\u7ebf\u6027\u653e\u5927\u5230\u5168\u91cf\uff1a\u4f4e\u4e30\u5ea6\u7269\u79cd\u3001\u590d\u6742\u5206\u652f\u548c\u957f\u91cd\u590d\u53ef\u80fd\u53ea\u5728\u6570\u636e\u589e\u5927\u540e\u51fa\u73b0\u3002\u57fa\u7ebf\u7684\u4f5c\u7528\u662f\u53d1\u73b0\u73af\u5883\u4e0e I\/O \u95ee\u9898\uff0c\u4e0d\u662f\u66ff\u4ee3\u5168\u91cf\u8d44\u6e90\u4f59\u91cf\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u5148\u786e\u8ba4\u6570\u636e\u6a21\u5f0f\uff0c\u518d\u5199\u547d\u4ee4<\/h2>\n<p>\u5f53\u524d metaMDBG \u4ed3\u5e93\u540c\u65f6\u652f\u6301 HiFi \u548c ONT\uff0c\u4f46\u8f93\u5165\u6a21\u5f0f\u4e0d\u540c\u3002Nanopore R10 simplex \u4f7f\u7528 <code>--in-ont<\/code>\uff0cPacBio HiFi \u4f7f\u7528 <code>--in-hifi<\/code>\u3002\u4e00\u4e2a\u53ef\u590d\u73b0\u7684 ONT \u8d77\u70b9\u5982\u4e0b\uff1a<\/p>\n<pre style=\"overflow-x:auto;padding:18px;background:#102f47;color:#f8fafc;border-radius:12px;line-height:1.55;\"><code class=\"language-bash\">metaMDBG asm \\\r\n  --in-ont reads.fastq.gz \\\r\n  --out-dir sample_nanomdbg \\\r\n  --threads 32\r\n<\/code><\/pre>\n<p>\u6b63\u5f0f\u8fd0\u884c\u524d\u81f3\u5c11\u8bb0\u5f55 metaMDBG \u7248\u672c\u3001reads \u603b\u78b1\u57fa\u6570\u3001\u957f\u5ea6\u5206\u5e03\u3001\u5e73\u5747\u8d28\u91cf\u3001\u8fc7\u6ee4\u9608\u503c\u3001\u7ebf\u7a0b\u6570\u548c\u5de5\u4f5c\u76ee\u5f55\u3002\u8bba\u6587\u57fa\u51c6\u4f7f\u7528\u7684\u662f v1.2\uff1b\u5b98\u65b9\u4ed3\u5e93\u5728 2026 \u5e74 5 \u6708\u53d1\u5e03 v1.4\uff0c\u5e76\u8bf4\u660e\u6709\u8fd0\u884c\u65f6\u95f4\u3001\u5185\u5b58\u548c polishing \u66f4\u65b0\u3002\u65b0\u7248\u672c\u53ef\u80fd\u66f4\u5feb\uff0c\u4f46\u4e0d\u80fd\u76f4\u63a5\u628a v1.2 \u7684\u6570\u5b57\u5f53\u4f5c v1.4 \u6216\u81ea\u5df1\u6837\u672c\u7684\u4fdd\u8bc1\u3002<\/p>\n<p>\u8f93\u5165\u8d28\u63a7\u8981\u907f\u514d\u4e24\u4e2a\u6781\u7aef\u3002\u5b8c\u5168\u4e0d\u770b adapters\u3001\u77ed reads \u548c\u4f4e\u8d28\u91cf\u5c3e\u90e8\uff0c\u4f1a\u628a\u65e0\u6548\u5e8f\u5217\u5e26\u5165\u7ea0\u9519\uff1b\u8fc7\u5ea6\u8fc7\u6ee4\u53c8\u53ef\u80fd\u635f\u5931\u4f4e\u4e30\u5ea6\u57fa\u56e0\u7ec4\u8986\u76d6\u3002\u81f3\u5c11\u4fdd\u5b58\u8fc7\u6ee4\u524d\u540e reads \u6570\u3001\u603b bases\u3001\u957f\u5ea6\u4e0e\u8d28\u91cf\u5206\u5e03\uff0c\u5e76\u628a\u8fc7\u6ee4\u547d\u4ee4\u5199\u5165\u65e5\u5fd7\u3002\u6d89\u53ca\u4eba\u6e90\u6216\u5bbf\u4e3b\u6837\u672c\u65f6\uff0c\u8fd8\u8981\u6839\u636e\u4f26\u7406\u548c\u5206\u6790\u76ee\u7684\u51b3\u5b9a\u5bbf\u4e3b\u8fc7\u6ee4\u53d1\u751f\u5728\u7ec4\u88c5\u524d\u8fd8\u662f\u7ed3\u679c\u9636\u6bb5\uff0c\u4e0d\u80fd\u9ed8\u8ba4\u628a\u654f\u611f reads \u5e26\u5165\u5171\u4eab\u73af\u5883\u3002<\/p>\n<p>\u73af\u5883\u4e5f\u5e94\u56fa\u5b9a\uff1a\u4fdd\u5b58 metaMDBG\u3001minimap2\u3001samtools \u7248\u672c\u53ca Conda\/\u5bb9\u5668\u6e05\u5355\uff1b\u540c\u4e00\u9879\u76ee\u4e0d\u8981\u4e2d\u9014\u5347\u7ea7 assembler\u3002<\/p>\n<p>ONT R9\u3001\u65e9\u671f\u9ad8\u9519\u8bef\u7387 reads\u3001\u8d85\u957f reads \u4e0e R10 simplex \u7684\u8bef\u5dee\u7ed3\u6784\u4e0d\u540c\u3002\u82e5\u6570\u636e\u7c7b\u578b\u4e0d\u5339\u914d\uff0c\u7b97\u6cd5\u4f18\u52bf\u53ef\u80fd\u6d88\u5931\u3002\u5bf9\u4e8e HiFi\uff0c\u5e94\u8be5\u8d70 metaMDBG \u7684 HiFi \u6a21\u5f0f\uff0c\u5e76\u4e0e hifiasm-meta\u3001metaFlye \u7684\u7ed3\u679c\u548c\u7814\u7a76\u76ee\u6807\u4e00\u8d77\u8bc4\u4f30\uff0c\u800c\u4e0d\u662f\u56e0\u4e3a nanoMDBG \u5728 ONT \u57fa\u51c6\u4e2d\u66f4\u7701\u8d44\u6e90\u5c31\u8de8\u5e73\u53f0\u5957\u7528\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u670d\u52a1\u5668\u89c4\u5212\u8981\u8986\u76d6\u201c\u7ec4\u88c5\u4e4b\u540e\u201d<\/h2>\n<p>\u539f\u59cb FASTQ \u53ea\u662f\u7b2c\u4e00\u4efd\u6570\u636e\u3002\u7ec4\u88c5\u76ee\u5f55\u3001\u7ea0\u9519 reads\u3001\u4e34\u65f6\u56fe\u3001contigs\u3001GFA\u3001reads-to-contigs BAM\u3001coverage \u8868\u548c MAG \u5206\u7bb1\u7ed3\u679c\u53ef\u80fd\u540c\u65f6\u5b58\u5728\u3002\u5c24\u5176\u662f reads \u56de\u8d34\uff0c\u5982\u679c\u5148\u5199\u5de8\u5927 SAM \u518d\u6392\u5e8f\uff0c\u4f1a\u4ea7\u751f\u4e0d\u5fc5\u8981\u7684 I\/O \u5cf0\u503c\u3002\u66f4\u5408\u7406\u7684\u662f\u6d41\u5f0f\u4f20\u7ed9 samtools\uff1a<\/p>\n<pre style=\"overflow-x:auto;padding:18px;background:#102f47;color:#f8fafc;border-radius:12px;line-height:1.55;\"><code class=\"language-bash\">minimap2 -ax map-ont -t 32 contigs.fasta.gz reads.fastq.gz \\\r\n  | samtools sort -@ 16 -o reads_vs_contigs.bam\r\nsamtools index reads_vs_contigs.bam\r\n<\/code><\/pre>\n<p>\u7ec4\u88c5\u5de5\u4f5c\u76ee\u5f55\u5e94\u653e\u5728\u541e\u5410\u7a33\u5b9a\u3001\u53ef\u6301\u4e45\u5316\u7684 SSD\/NVMe\uff1b\u4e34\u65f6\u6587\u4ef6\u53ef\u7528\u672c\u5730 scratch\uff0c\u4f46 checkpoint \u548c\u5173\u952e\u4e2d\u95f4\u6587\u4ef6\u4e0d\u80fd\u53ea\u653e\u4f1a\u81ea\u52a8\u6e05\u7406\u7684\u4e34\u65f6\u76d8\u3002\u5171\u4eab\u6587\u4ef6\u7cfb\u7edf\u62e5\u5835\u65f6\uff0cCPU \u4f7f\u7528\u7387\u4f1a\u4e0b\u964d\uff0c\u4efb\u52a1\u770b\u4f3c\u201c\u8fd8\u5728\u8dd1\u201d\u5374\u957f\u671f\u7b49\u5f85 I\/O\u3002\u5148\u76d1\u63a7\u8bfb\u5199\u5e26\u5bbd\u548c\u961f\u5217\uff0c\u518d\u51b3\u5b9a\u589e\u52a0\u7ebf\u7a0b\u3002<\/p>\n<p>\u5efa\u8bae\u5728\u57fa\u7ebf\u4efb\u52a1\u5916\u5c42\u4f7f\u7528 <code>\/usr\/bin\/time -v<\/code> \u8bb0\u5f55\u5cf0\u503c\u5185\u5b58\u4e0e\u5899\u949f\u65f6\u95f4\uff0c\u540c\u65f6\u5b9a\u671f\u91c7\u96c6 <code>iostat<\/code>\u3001\u5269\u4f59\u7a7a\u95f4\u548c\u8f93\u51fa\u76ee\u5f55\u589e\u957f\u3002\u53ea\u770b CPU \u767e\u5206\u6bd4\u65e0\u6cd5\u533a\u5206\u8ba1\u7b97\u3001I\/O \u7b49\u5f85\u548c\u5185\u5b58\u6362\u9875\uff1b\u5982\u679c swap \u5f00\u59cb\u6301\u7eed\u589e\u957f\uff0c\u5e94\u5148\u505c\u6b62\u653e\u5927\u5168\u91cf\u4efb\u52a1\u3002\u8f93\u51fa\u5b8c\u6210\u540e\u4e3a\u539f\u59cb reads\u3001contigs\u3001\u914d\u7f6e\u548c\u5173\u952e\u62a5\u544a\u751f\u6210\u6821\u9a8c\u503c\uff0c\u8fc1\u79fb\u5230\u5f52\u6863\u76d8\u540e\u518d\u6e05\u7406\u5de5\u4f5c\u76ee\u5f55\u3002<\/p>\n<p>\u591a\u6837\u672c\u5e76\u53d1\u4e5f\u8981\u6309\u5cf0\u503c\u7b97\u3002\u4e24\u4efd\u5404\u9700 125 GB \u7684\u571f\u58e4\u4efb\u52a1\u540c\u65f6\u8fd0\u884c\uff0c\u4e0d\u80fd\u53ea\u7533\u8bf7\u4e00\u53f0 256 GB \u8282\u70b9\u540e\u8ba9\u7cfb\u7edf\u81ea\u7531\u7ade\u4e89\uff1b\u8fd8\u8981\u7ed9\u64cd\u4f5c\u7cfb\u7edf\u3001\u6587\u4ef6\u7f13\u5b58\u3001\u56de\u8d34\u548c\u4e0b\u6e38\u6d41\u7a0b\u7559\u4f59\u91cf\u3002\u957f\u8fbe\u6570\u5929\u7684\u4efb\u52a1\u5e94\u8bbe\u7f6e\u65e5\u5fd7\u3001\u78c1\u76d8\u544a\u8b66\u548c\u5931\u8d25\u6062\u590d\u68c0\u67e5\uff0c\u907f\u514d\u5728\u7b2c 5 \u5929\u56e0\u7a7a\u95f4\u5199\u6ee1\u91cd\u8dd1\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u66f4\u7701\u5185\u5b58\uff0c\u4e0d\u7b49\u4e8e\u53ef\u4ee5\u8df3\u8fc7\u7ec4\u88c5\u8d28\u63a7<\/h2>\n<p>2025 \u5e74 Nature Biotechnology \u5bf9 21 \u4e2a HiFi \u5b8f\u57fa\u56e0\u7ec4\u548c\u591a\u79cd assembler \u7684\u590d\u67e5\u6307\u51fa\uff0c\u957f\u8bfb\u957f\u7ec4\u88c5\u53ef\u51fa\u73b0\u6bcf 1 \u4ebf bp \u8d85\u8fc7 <strong>40 \u4e2a\u9519\u8bef<\/strong>\uff0c\u5305\u62ec\u5d4c\u5408\u3001\u8fc7\u65e9\u73af\u5316\u3001\u5355\u500d\u578b\u9519\u8bef\u3001\u91cd\u590d\u548c phantom sequence\u3002\u8bba\u6587\u7279\u522b\u63d0\u9192\uff1a\u62a5\u544a\u4e3a circular \u7684 contig \u4e5f\u53ef\u80fd\u7f3a\u5931\u6838\u5fc3\u57fa\u56e0\u7ec4\u7247\u6bb5\u3002<\/p>\n<p>\u8d28\u91cf\u9a8c\u6536\u6700\u597d\u5206\u4e09\u5c42\u3002\u7b2c\u4e00\u5c42\u770b reads \u56de\u8d34\u7387\u3001split alignment\u3001\u96f6\u8986\u76d6\u533a\u548c\u8986\u76d6\u7a81\u53d8\uff0c\u5224\u65ad contig \u662f\u5426\u6709\u76f4\u63a5 read \u652f\u6301\uff1b\u7b2c\u4e8c\u5c42\u770b CheckM2\u3001GUNC \u7b49\u5b8c\u6574\u6027\u4e0e\u6c61\u67d3\u6307\u6807\uff0c\u5224\u65ad MAG \u662f\u5426\u50cf\u4e00\u4e2a\u4e00\u81f4\u7684\u57fa\u56e0\u7ec4\uff1b\u7b2c\u4e09\u5c42\u770b GTDB \u5206\u7c7b\u3001\u6838\u5fc3\u4ee3\u8c22\u4e0e\u86cb\u767d\u5b8c\u6574\u6027\uff0c\u5224\u65ad\u7ed3\u679c\u662f\u5426\u7b26\u5408\u751f\u7269\u5b66\u80cc\u666f\u3002\u4efb\u4f55\u5355\u4e00\u6307\u6807\u90fd\u4e0d\u80fd\u66ff\u4ee3\u53e6\u5916\u4e24\u5c42\u3002<\/p>\n<p>nanoMDBG \u7684 2026 \u8bba\u6587\u540c\u6837\u663e\u793a\u65b9\u6cd5\u4e4b\u95f4\u6709\u53d6\u820d\u3002ONT \u957f contig \u7684\u6c61\u67d3\u6bd4\u4f8b\u4e2d\u4f4d\u6570\u90fd\u8f83\u4f4e\uff0c\u4f46 metaFlye \u7684\u6c61\u67d3\u957f contig \u4e3a 2\/326\uff0cnanoMDBG \u4e3a 13\/1006\uff1b\u53e6\u4e00\u65b9\u9762\uff0cnanoMDBG \u7684 clipping events\u3001\u96f6\u8986\u76d6\u533a\u57df\u548c\u5168\u957f\u86cb\u767d\u6307\u6807\u5728\u591a\u4e2a\u6570\u636e\u96c6\u4e0a\u4f18\u4e8e metaFlye\u3002\u7ed3\u8bba\u4e0d\u662f\u201c\u4e00\u4e2a\u5de5\u5177\u6c38\u8fdc\u6700\u597d\u201d\uff0c\u800c\u662f\u7ec4\u88c5\u540e\u5fc5\u987b\u628a reads \u56de\u8d34\uff0c\u68c0\u67e5 clipping\u3001\u96f6\u8986\u76d6\u3001\u6c61\u67d3\u3001\u5b8c\u6574\u6027\u548c\u57fa\u56e0\u9884\u6d4b\u3002<\/p>\n<p>\u5706\u5f62 contig \u4e5f\u4e0d\u80fd\u76f4\u63a5\u7b49\u540c\u4e8e\u5b8c\u6574\u67d3\u8272\u4f53\u6216\u8d28\u7c92\u3002\u81f3\u5c11\u8981\u6838\u5bf9\u8986\u76d6\u662f\u5426\u8fde\u7eed\u3001\u672b\u7aef\u662f\u5426\u6709\u5f02\u5e38 reads split\u3001\u5355\u62f7\u8d1d\u6838\u5fc3\u57fa\u56e0\u662f\u5426\u5b8c\u6574\uff0c\u4ee5\u53ca\u8fd1\u7f18\u53c2\u8003\u4e2d\u5e94\u5b58\u5728\u7684\u6838\u5fc3\u529f\u80fd\u662f\u5426\u7f3a\u5931\u3002\u5bf9\u5173\u952e MAG\uff0c\u5efa\u8bae\u4fdd\u7559\u591a assembler \u7ed3\u679c\u6216\u81f3\u5c11\u505a\u9488\u5bf9\u6027\u590d\u6838\u3002<\/p>\n<p>\u5982\u679c\u9879\u76ee\u76ee\u6807\u662f\u627e\u75c5\u6bd2\u3001\u8d28\u7c92\u6216\u7279\u5b9a\u4f4e\u4e30\u5ea6\u7269\u79cd\uff0c\u8bc4\u4ef7\u6807\u51c6\u8fd8\u8981\u8c03\u6574\u3002\u8ffd\u6c42\u6700\u957f contig \u53ef\u80fd\u727a\u7272\u4fdd\u5b88\u6027\uff1b\u8ffd\u6c42\u6700\u4f4e\u6c61\u67d3\u53c8\u53ef\u80fd\u51cf\u5c11\u53ef\u6062\u590d\u5e8f\u5217\u3002\u5e94\u5728\u7ec4\u88c5\u524d\u5199\u6e05\u4e3b\u8981\u4ea4\u4ed8\u662f\u9ad8\u8d28\u91cf MAG\u3001\u79fb\u52a8\u9057\u4f20\u5143\u4ef6\u3001\u57fa\u56e0\u76ee\u5f55\u8fd8\u662f\u7fa4\u843d\u6bd4\u8f83\uff0c\u518d\u51b3\u5b9a\u662f\u5426\u5e76\u884c\u8dd1\u7b2c\u4e8c\u4e2a assembler\uff0c\u4ee5\u53ca\u54ea\u4e9b contig \u503c\u5f97\u4eba\u5de5\u590d\u6838\u3002<\/p>\n<p>\u5982\u679c\u4f60\u7684\u957f\u8bfb\u957f\u5b8f\u57fa\u56e0\u7ec4\u5361\u5728 nanoMDBG\/metaFlye \u9009\u62e9\u3001125\/256\/768 GB \u5185\u5b58\u914d\u7f6e\u3001400 Gbp \u5de5\u4f5c\u76d8\u3001\u957f\u4efb\u52a1\u8c03\u5ea6\u3001reads \u56de\u8d34\u6216 MAG \u8d28\u63a7\uff0c\u53ef\u4ee5\u51c6\u5907\u6d4b\u5e8f\u5e73\u53f0\u4e0e chemistry\u3001reads \u603b\u91cf\u548c\u8d28\u91cf\u3001\u6837\u672c\u7c7b\u578b\u3001\u73b0\u6709\u670d\u52a1\u5668\u548c\u76ee\u6807\u4ea4\u4ed8\u3002\u4e91\u751f\u4fe1\u53ef\u5148\u505a\u5de5\u5177\u9002\u914d\u4e0e\u5c0f\u89c4\u6a21\u57fa\u7ebf\uff0c\u518d\u786e\u8ba4\u79df\u7528\u73af\u5883\u81ea\u884c\u8fd0\u884c\u3001\u670d\u52a1\u5668\u90e8\u7f72\u4ea4\u63a5\u6216\u59d4\u6258\u5206\u6790\u7684\u8303\u56f4\u3002<\/p>\n<p><a href=\"https:\/\/work.weixin.qq.com\/kfid\/kfcb05492f29d4d6064\" data-ysx-event=\"contact_click\" style=\"display:inline-block;padding:13px 20px;border-radius:10px;background:#e36e2e;color:#fff;text-decoration:none;font-weight:800;\">\u8054\u7cfb\u4e91\u751f\u4fe1\uff0c\u54a8\u8be2\u957f\u8bfb\u957f\u5b8f\u57fa\u56e0\u7ec4\u7ec4\u88c5\u670d\u52a1\u5668\u4e0e\u5206\u6790\u65b9\u6848<\/a><\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u53c2\u8003\u8d44\u6599<\/h2>\n<ul>\n<li><a href=\"https:\/\/www.nature.com\/articles\/s41467-026-69760-y\">nanoMDBG \u539f\u59cb\u8bba\u6587\uff1aHigh-quality metagenome assembly from nanopore reads<\/a><\/li>\n<li><a href=\"https:\/\/github.com\/GaetanBenoitDev\/metaMDBG\">metaMDBG \u5b98\u65b9\u4ed3\u5e93\u4e0e\u5f53\u524d\u8fd0\u884c\u65b9\u5f0f<\/a><\/li>\n<li><a href=\"https:\/\/www.nature.com\/articles\/s41587-025-02971-8\">\u957f\u8bfb\u957f\u5b8f\u57fa\u56e0\u7ec4\u7ec4\u88c5\u9519\u8bef\u57fa\u51c6<\/a><\/li>\n<li><a href=\"https:\/\/www.nature.com\/articles\/s41587-023-01983-6\">metaMDBG HiFi \u539f\u59cb\u8bba\u6587\u4e0e\u8d44\u6e90\u57fa\u51c6<\/a><\/li>\n<\/ul>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>400Gbp Nanopore\u571f\u58e4\u5b8f\u57fa\u56e0\u7ec4\u4e2d\uff0cnanoMDBG\u8bba\u6587\u57fa\u51c6\u4e3a6\u5929\u3001125GB\u5185\u5b58\uff0cmetaFlye\u4e3a28\u5929\u3001753GB\u3002\u672c\u6587\u62c6\u89e3\u5de5\u5177\u9009\u62e9\u3001\u5de5\u4f5c\u76d8\u3001\u56de\u8d34\u8d28\u63a7\u4e0e\u670d\u52a1\u5668\u89c4\u5212\u3002<\/p>\n","protected":false},"author":1,"featured_media":66312,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[1,75],"tags":[385,441,438,437,439,383,440,431],"class_list":["post-66313","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-part","category-compute-environment","tag-mag","tag-metaflye","tag-metamdbg","tag-nanomdbg","tag-nanopore","tag-383","tag-440","tag-431"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/10\/daily-bio-blog-20261029-nanomdbg-metagenome-nanomdbg-poster.png?fit=1080%2C1350&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66313","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=66313"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66313\/revisions"}],"predecessor-version":[{"id":66314,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66313\/revisions\/66314"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/66312"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=66313"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=66313"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=66313"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}