{"id":66414,"date":"2026-11-13T09:13:19","date_gmt":"2026-11-13T01:13:19","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66414"},"modified":"2026-11-13T09:13:19","modified_gmt":"2026-11-13T01:13:19","slug":"fastqc-red-flags-five-modules-diagnosis","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/fastqc-red-flags-five-modules-diagnosis\/","title":{"rendered":"FastQC\u4e00\u7247\u7ea2\u5c31\u662f\u6570\u636e\u5e9f\u4e86\uff1f\u5148\u770b\u8fd95\u4e2a\u6a21\u5757\uff0c\u518d\u51b3\u5b9a\u8981\u4e0d\u8981\u91cd\u505a"},"content":{"rendered":"
\n

\u6253\u5f00FastQC\u62a5\u544a\uff0c\u5de6\u4fa7\u8fde\u7eed\u51fa\u73b0\u7ea2\u53c9\uff0c\u5f88\u591a\u4eba\u7b2c\u4e00\u53cd\u5e94\u662f\u201c\u8fd9\u6279\u6570\u636e\u4e0d\u80fd\u7528\u4e86\u201d\u3002\u4f46FastQC\u7684\u7ea2\u3001\u9ec4\u3001\u7eff\u53ea\u662f\u6309\u901a\u7528\u9608\u503c\u89e6\u53d1\u7684\u63d0\u793a\uff0c\u4e0d\u77e5\u9053\u4f60\u7684\u5efa\u5e93\u7c7b\u578b\u3001\u7814\u7a76\u76ee\u6807\u548c\u4e0b\u6e38\u65b9\u6cd5\u3002RNA-seq\u5f00\u5934\u78b1\u57fa\u6bd4\u4f8b\u4e0d\u5747\u3001small RNA\u91cd\u590d\u5ea6\u9ad8\u3001\u4e9a\u786b\u9178\u6c22\u76d0\u6d4b\u5e8fC\/T\u6bd4\u4f8b\u5f02\u5e38\uff0c\u90fd\u53ef\u80fd\u662f\u5b9e\u9a8c\u8bbe\u8ba1\u5e26\u6765\u7684\u9884\u671f\u73b0\u8c61\u3002<\/p>\n

\u771f\u6b63\u6709\u7528\u7684\u5224\u65ad\u4e0d\u662f\u6570\u7ea2\u53c9\uff0c\u800c\u662f\u56de\u7b54\u4e09\u4e2a\u95ee\u9898\uff1a\u5f02\u5e38\u53d1\u751f\u5728\u54ea\u4e2a\u4f4d\u7f6e\uff1f\u591a\u4e2a\u6a21\u5757\u662f\u5426\u6307\u5411\u540c\u4e00\u4e2a\u539f\u56e0\uff1f\u5b83\u4f1a\u4e0d\u4f1a\u5f71\u54cd\u540e\u7eed\u6bd4\u5bf9\u3001\u5b9a\u91cf\u6216\u53d8\u5f02\u68c0\u6d4b\uff1f<\/p>\n

\"FastQC\u7ea2\u53c9\u8bca\u65ad\u6d77\u62a5\uff1a\u6309\u8d28\u91cf\u3001\u63a5\u5934\u3001\u91cd\u590d\u3001\u78b1\u57fa\u7ec4\u6210\u548cGC\u5206\u5e03\u5224\u65ad\u662f\u5426\u9700\u8981\u5904\u7406\"
FastQC\u7ea2\u53c9\u8bca\u65ad\uff1a\u7ed3\u5408\u5efa\u5e93\u7c7b\u578b\u3001\u6a21\u5757\u7ec4\u5408\u548c\u540c\u6279\u6837\u672c\u5224\u65ad\uff0c\u4e0d\u4ee5\u7ea2\u53c9\u6570\u91cf\u5224\u5b9a\u6570\u636e\u4f5c\u5e9f\u3002<\/figcaption><\/figure>\n

\u7248\u672c\u3001\u8f93\u5165\u548c\u8f93\u51fa\u5148\u6838\u5bf9<\/h2>\n

\u672c\u6587\u4e8e2026\u5e7411\u670813\u65e5\u6838\u5bf9FastQC\u5b98\u65b9\u8d44\u6599\uff0c\u5f53\u524d\u6700\u65b0\u53d1\u5e03\u7248\u4e3a0.13.0\u3002\u8fd9\u4e2a\u7248\u672c\u7684\u542f\u52a8\u811a\u672c\u6539\u7528Python 3\uff0c\u8981\u6c42Python 3.7\u53ca\u4ee5\u4e0a\u3001Java 8\u53ca\u4ee5\u4e0a\uff1b\u7ebf\u7a0b\u6a21\u578b\u4e5f\u5df2\u66f4\u65b0\uff0c\u5355\u4e2aFASTQ\u6700\u591a\u53ef\u4f7f\u75284\u4e2a\u7ebf\u7a0b\uff0c\u5e76\u80fd\u5e76\u884c\u5904\u7406\u591a\u4e2a\u6587\u4ef6\u3002\u65e7\u6559\u7a0b\u91cc\u201c\u4e00\u4e2a\u7ebf\u7a0b\u53ea\u5bf9\u5e94\u4e00\u4e2a\u6587\u4ef6\u201d\u7684\u7ecf\u9a8c\u4e0d\u80fd\u76f4\u63a5\u5957\u52300.13.0\u3002<\/p>\n

FastQC\u53ef\u8bfb\u53d6FASTQ\uff0c\u4e5f\u652f\u6301SAM\/BAM\u3002\u5e38\u89c1\u539f\u59cb\u6570\u636e\u76f4\u63a5\u8fd0\u884c\uff1a<\/p>\n

mkdir -p fastqc_out\r\nfastqc --threads 8 --outdir fastqc_out sample_R1.fastq.gz sample_R2.fastq.gz\r\n<\/code><\/pre>\n
\u6bcf\u4e2a\u8f93\u5165\u6587\u4ef6\u4f1a\u5f97\u5230\u4e00\u4e2aHTML\u62a5\u544a\u548c\u4e00\u4e2aZIP\u5305\uff1b\u89e3\u538b\u540e\u53ef\u770b\u5230\u673a\u5668\u53ef\u8bfb\u7684summary.txt<\/code>\u3001fastqc_data.txt<\/code>\u548c\u56fe\u8868\u6587\u4ef6\u3002\u6210\u5bf9\u6d4b\u5e8f\u7684R1\u3001R2\u8981\u5206\u522b\u68c0\u67e5\uff0c\u4e0d\u80fd\u53ea\u62bd\u770b\u5176\u4e2d\u4e00\u4e2a\u3002\u8fd0\u884c\u6210\u529f\u81f3\u5c11\u5e94\u6ee1\u8db3\uff1a\u547d\u4ee4\u65e0\u89e3\u6790\u9519\u8bef\u3001HTML\u53ef\u6253\u5f00\u3001Basic Statistics\u4e2d\u7684\u6587\u4ef6\u540d\u4e0eread\u6570\u7b26\u5408\u9884\u671f\u3001R1\/R2\u62a5\u544a\u9f50\u5168\u3002<\/p>\n
\u4e94\u4e2a\u6a21\u5757\uff0c\u5e94\u8be5\u600e\u6837\u8054\u5408\u5224\u65ad<\/h2>\n
\n\n\n\n\n\n\n\n\n\n
\u6a21\u5757<\/th>\n\u5b98\u65b9\u89e6\u53d1\u6761\u4ef6\uff08\u8282\u9009\uff09<\/th>\n\u770b\u5230\u7ea2\u9ec4\u540e\u5148\u95ee\u4ec0\u4e48<\/th>\n<\/tr>\n<\/thead>\n
Per base sequence quality<\/td>\n\u4efb\u4e00\u4f4d\u7f6e\u4e0b\u56db\u5206\u4f4d\u6570\uff1c10\u6216\u4e2d\u4f4d\u6570\uff1c25\u8b66\u544a\uff1b\u4e0b\u56db\u5206\u4f4d\u6570\uff1c5\u6216\u4e2d\u4f4d\u6570\uff1c20\u5931\u8d25<\/td>\n\u662f\u672b\u7aef\u9010\u6e10\u4e0b\u964d\uff0c\u8fd8\u662f\u4e2d\u95f4\u77ed\u6682\u584c\u9677\uff1f\u540e\u8005\u4e0d\u5b9c\u76f4\u63a5\u6574\u6bb5\u526a\u5207<\/td>\n<\/tr>\n
Adapter Content<\/td>\n\u4efb\u4e00\u63a5\u5934\u7d2f\u8ba1\u51fa\u73b0\u5728\uff1e5% reads\u4e2d\u8b66\u544a\uff0c\uff1e10%\u5931\u8d25<\/td>\n\u662f\u5426\u4eceread\u672b\u7aef\u6301\u7eed\u4e0a\u5347\uff1f\u63d2\u5165\u7247\u6bb5\u662f\u5426\u77ed\u4e8e\u8bfb\u957f\uff1f<\/td>\n<\/tr>\n
Overrepresented sequences<\/td>\n\u5355\u6761\u5e8f\u5217\uff1e0.1%\u8b66\u544a\uff0c\uff1e1%\u5931\u8d25<\/td>\n\u5339\u914d\u7684\u662f\u63a5\u5934\u3001rRNA\u3001\u5f15\u7269\uff0c\u8fd8\u662f\u9884\u671f\u9ad8\u4e30\u5ea6\u751f\u7269\u5e8f\u5217\uff1f<\/td>\n<\/tr>\n
Per base sequence content<\/td>\n\u4efb\u4e00\u4f4d\u7f6eA\/T\u6216G\/C\u5dee\u5f02\uff1e10%\u8b66\u544a\uff0c\uff1e20%\u5931\u8d25<\/td>\nRNA-seq\u524d\u7ea612 bp\u504f\u501a\u662f\u5426\u6765\u81ea\u968f\u673a\u5f15\u7269\uff1f\u5efa\u5e93\u7c7b\u578b\u662f\u5426\u672c\u6765\u5c31\u504f\uff1f<\/td>\n<\/tr>\n
Per sequence GC content<\/td>\n\u76f8\u5bf9\u62df\u5408\u6b63\u6001\u5206\u5e03\u7684\u603b\u504f\u5dee\uff1e15%\u8b66\u544a\uff0c\uff1e30%\u5931\u8d25<\/td>\n\u662f\u5c16\u9510\u6c61\u67d3\u5cf0\u3001\u6df7\u5408\u7269\u79cd\u5bbd\u5cf0\uff0c\u8fd8\u662f\u76ee\u6807\u751f\u7269\u672c\u8eabGC\u504f\u79fb\uff1f<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u8fd9\u4e9b\u9608\u503c\u9002\u5408\u505a\u7edf\u4e00\u62a5\u8b66\uff0c\u5374\u4e0d\u662f\u901a\u7528\u6dd8\u6c70\u7ebf\u3002\u4f8b\u5982FastQC\u5b98\u65b9\u8bf4\u660e\uff0c\u51e0\u4e4e\u6240\u6709\u968f\u673a\u516d\u805a\u4f53\u5f15\u7269RNA-seq\u90fd\u53ef\u80fd\u57285\u2032\u7aef\u51fa\u73b0\u7ec4\u6210\u504f\u501a\uff0c\u8fd9\u901a\u5e38\u4e0d\u80fd\u9760\u526a\u5207\u201c\u4fee\u597d\u201d\uff0c\u4e5f\u672a\u5fc5\u59a8\u788d\u8868\u8fbe\u5b9a\u91cf\u3002\u76f8\u53cd\uff0c\u5982\u679c\u8d28\u91cf\u5c3e\u90e8\u4e0b\u964d\u3001Adapter Content\u540c\u6b65\u4e0a\u5347\u3001Overrepresented sequences\u53c8\u547d\u4e2d\u63a5\u5934\uff0c\u4e09\u4e2a\u6a21\u5757\u5171\u540c\u652f\u6301\u201c\u5b58\u5728\u63a5\u5934\u8bfb\u7a7f\u201d\uff0c\u518d\u8003\u8651\u526a\u5207\u5c31\u66f4\u6709\u4f9d\u636e\u3002<\/p>\n
\u4e00\u5957\u53ef\u590d\u7528\u7684\u8bca\u65ad\u987a\u5e8f<\/h2>\n
\u7b2c\u4e00\u6b65\uff0c\u5148\u770bBasic Statistics\u4e0eSequence Length Distribution\u3002\u786e\u8ba4\u6587\u4ef6\u4e0d\u662f\u7a7a\u7684\u3001read\u957f\u5ea6\u7b26\u5408\u5e73\u53f0\u4e0e\u526a\u5207\u5386\u53f2\u3001R1\/R2\u6570\u91cf\u548c\u547d\u540d\u6ca1\u6709\u6df7\u4e71\u3002\u57fa\u7840\u4fe1\u606f\u9519\u4e86\uff0c\u540e\u9762\u7684\u7ea2\u7eff\u6ca1\u6709\u89e3\u91ca\u4ef7\u503c\u3002<\/p>\n
\u7b2c\u4e8c\u6b65\uff0c\u770bPer base sequence quality\u548cPer tile sequence quality\u3002\u672b\u7aef\u7f13\u6162\u53d8\u5dee\u5e38\u89c1\u4e8e\u957f\u8bfb\u957f\u5316\u5b66\u8d28\u91cf\u8870\u51cf\uff1b\u5982\u679c\u53ea\u5728\u67d0\u4e9btile\u51fa\u73b0\u5c40\u90e8\u4f4e\u8d28\u91cf\uff0c\u66f4\u50cf\u6d4b\u5e8f\u9762\u677f\u95ee\u9898\u3002\u5b98\u65b9\u63d0\u793a\uff0c\u4e2d\u6bb5\u77ed\u6682\u6389\u8d28\u540e\u53c8\u6062\u590d\u65f6\uff0c\u76f4\u63a5\u4ece\u8be5\u70b9\u622a\u65ad\u4f1a\u8bef\u5220\u540e\u9762\u4ecd\u7136\u826f\u597d\u7684\u5e8f\u5217\u3002<\/p>\n
\u7b2c\u4e09\u6b65\uff0c\u628aAdapter Content\u3001Overrepresented sequences\u548c\u78b1\u57fa\u7ec4\u6210\u653e\u5728\u4e00\u8d77\u3002\u63a5\u5934\u66f2\u7ebf\u4ece\u540e\u534a\u6bb5\u7d2f\u79ef\u4e0a\u5347\uff0c\u624d\u652f\u6301read-through\uff1b\u5355\u4e00\u9ad8\u4e30\u5ea6\u5e8f\u5217\u8fd8\u8981\u7ed3\u5408\u6c61\u67d3\u7269\u5339\u914d\u548c\u5b9e\u9a8c\u7c7b\u578b\u89e3\u91ca\u3002FastQC\u53ea\u8ddf\u8e2a\u524d100,000\u6761read\u4e2d\u51fa\u73b0\u7684\u5e8f\u5217\u7528\u4e8e\u8fc7\u8868\u8fbe\u7edf\u8ba1\uff0c\u62a5\u544a\u6ca1\u6709\u547d\u4e2d\u4e5f\u4e0d\u7b49\u4e8e\u5b8c\u5168\u4e0d\u5b58\u5728\u6c61\u67d3\u3002<\/p>\n
\u7b2c\u56db\u6b65\uff0c\u6309\u540c\u4e00\u6279\u6b21\u6bd4\u8f83\u6837\u672c\u3002\u5355\u4e2aRNA-seq\u6837\u672c5\u2032\u7aef\u504f\u501a\u53ef\u80fd\u6b63\u5e38\uff0c\u4f46\u82e5\u53ea\u6709\u4e00\u4e2a\u6837\u672cGC\u5206\u5e03\u51fa\u73b0\u989d\u5916\u5c16\u5cf0\u3001\u63a5\u5934\u6bd4\u4f8b\u660e\u663e\u504f\u79bb\u5176\u4ed6\u6837\u672c\uff0c\u5c31\u503c\u5f97\u56de\u67e5\u5efa\u5e93\u4e0e\u6837\u672c\u8eab\u4efd\u3002\u6279\u91cf\u9879\u76ee\u53ef\u7528MultiQC\u6c47\u603bFastQC\u7ed3\u679c\uff0c\u4f46\u539f\u59cb\u5224\u65ad\u4ecd\u5e94\u56de\u5230FastQC\u5355\u6837\u672c\u66f2\u7ebf\u3002<\/p>\n
\u7b2c\u4e94\u6b65\uff0c\u5904\u7406\u524d\u540e\u5404\u8dd1\u4e00\u6b21\u3002\u526a\u5207\u540e\u5e94\u5173\u6ce8\u63a5\u5934\u66f2\u7ebf\u662f\u5426\u4e0b\u964d\u3001\u957f\u5ea6\u5206\u5e03\u662f\u5426\u7b26\u5408\u9884\u671f\u3001\u4f4e\u8d28\u91cf\u5c3e\u90e8\u662f\u5426\u6539\u5584\uff1b\u4e0d\u80fd\u53ea\u8ffd\u6c42\u5168\u90e8\u53d8\u7eff\u3002\u8fc7\u5ea6\u526a\u5207\u4f1a\u7f29\u77edread\u3001\u4e22\u5931\u6709\u6548\u4fe1\u606f\uff0c\u751a\u81f3\u5f15\u5165\u65b0\u7684\u672b\u7aef\u7ec4\u6210\u504f\u501a\u3002<\/p>\n
\u6279\u91cf\u8fd0\u884c\u65f6\uff0c\u5efa\u8bae\u628a\u8f93\u5165\u6e05\u5355\u3001\u8f6f\u4ef6\u7248\u672c\u3001\u547d\u4ee4\u548c\u9000\u51fa\u72b6\u6001\u4e00\u8d77\u7559\u5b58\uff0c\u800c\u4e0d\u662f\u53ea\u4fdd\u7559HTML\u3002\u4e0b\u9762\u7684\u5199\u6cd5\u53ef\u907f\u514d\u8f93\u51fa\u76ee\u5f55\u7f3a\u5931\uff0c\u5e76\u628a\u6807\u51c6\u8f93\u51fa\u548c\u9519\u8bef\u4fe1\u606f\u5199\u5165\u65e5\u5fd7\uff1a<\/p>\n
mkdir -p fastqc_raw logs\r\nfastqc --threads 8 --outdir fastqc_raw data\/*.fastq.gz \\\r\n  > logs\/fastqc_raw.log 2>&1\r\ntest $? -eq 0 && echo "FastQC finished"\r\n<\/code><\/pre>\n
\u5982\u679c\u6837\u672c\u5f88\u591a\uff0c\u5148\u75282\u20144\u4e2a\u6587\u4ef6\u505a\u5c0f\u6279\u8bd5\u8dd1\uff0c\u89c2\u5bdfCPU\u5229\u7528\u7387\u3001\u78c1\u76d8\u8bfb\u5199\u548c\u5355\u6587\u4ef6\u8017\u65f6\uff0c\u518d\u51b3\u5b9a\u5e76\u53d1\u6570\u3002\u5b8c\u6210\u540e\u68c0\u67e5\u8f93\u5165FASTQ\u6570\u3001HTML\u6570\u548cZIP\u6570\u662f\u5426\u4e00\u81f4\uff1b\u7f3a\u4e00\u4efd\u62a5\u544a\u5c31\u5e94\u5148\u5b9a\u4f4d\u5931\u8d25\u6587\u4ef6\uff0c\u4e0d\u80fd\u628a\u201c\u591a\u6570\u6837\u672c\u5b8c\u6210\u201d\u5f53\u6210\u6574\u6279\u6210\u529f\u3002\u4e4b\u540e\u518d\u7528MultiQC\u751f\u6210\u9879\u76ee\u7ea7\u603b\u89c8\uff0c\u5e76\u4fdd\u7559\u5f02\u5e38\u6837\u672c\u7684\u539f\u59cbFastQC\u62a5\u544a\u7528\u4e8e\u8ffd\u6eaf\u3002<\/p>\n
\u4ea4\u4ed8\u6216\u6362\u673a\u5668\u590d\u8dd1\u65f6\uff0c\u8fd8\u5e94\u4fdd\u7559\u8f93\u5165\u6587\u4ef6\u6821\u9a8c\u503c\u4e0eFastQC\u7248\u672c\u53f7\u3002\u8fd9\u6837\u65e2\u80fd\u533a\u5206\u201c\u6570\u636e\u53d1\u751f\u53d8\u5316\u201d\u548c\u201c\u8f6f\u4ef6\u73af\u5883\u53d1\u751f\u53d8\u5316\u201d\uff0c\u4e5f\u65b9\u4fbf\u5728\u4e0b\u6e38\u7ed3\u679c\u5f02\u5e38\u65f6\u56de\u5230\u539f\u59cb\u8d28\u63a7\u8bc1\u636e\uff0c\u800c\u4e0d\u662f\u91cd\u65b0\u731c\u6d4b\u95ee\u9898\u4ece\u54ea\u4e00\u6b65\u5f00\u59cb\u3002<\/p>\n
\u54ea\u4e9b\u60c5\u51b5\u672c\u5730\u591f\u7528\uff0c\u4f55\u65f6\u518d\u4e0a\u670d\u52a1\u5668<\/h2>\n
\u4e24\u4e09\u4e2aFASTQ\u3001\u6587\u4ef6\u4e0d\u5927\u3001\u53ea\u9700\u4eba\u5de5\u67e5\u770bHTML\u65f6\uff0c\u672c\u5730\u7535\u8111\u901a\u5e38\u8db3\u591f\u3002\u5148\u628a\u7248\u672c\u3001\u547d\u4ee4\u548c\u5224\u8bfb\u6807\u51c6\u56fa\u5b9a\uff0c\u6bd4\u76f4\u63a5\u589e\u52a0\u786c\u4ef6\u66f4\u91cd\u8981\u3002FastQC\u4e5f\u4e0d\u662fGPU\u8f6f\u4ef6\uff0c\u6362\u663e\u5361\u4e0d\u4f1a\u89e3\u51b3\u8d28\u63a7\u95ee\u9898\u3002<\/p>\n
\u5f53\u9879\u76ee\u6709\u51e0\u5341\u5230\u4e0a\u767e\u4e2a\u538b\u7f29FASTQ\u3001\u9700\u8981\u539f\u59cb\u4e0e\u526a\u5207\u540e\u4e24\u8f6e\u8d28\u63a7\u3001\u8fd8\u8981\u957f\u671f\u4fdd\u7559HTML\u3001ZIP\u3001\u65e5\u5fd7\u548c\u6c47\u603b\u7ed3\u679c\u65f6\uff0c\u74f6\u9888\u66f4\u5e38\u5728\u78c1\u76d8\u541e\u5410\u3001\u5e76\u53d1\u8c03\u5ea6\u4e0e\u7a33\u5b9a\u8fd0\u884c\u3002\u6b64\u65f6\u670d\u52a1\u5668\u7684\u4ef7\u503c\u662f\u8ba9\u6279\u91cf\u4efb\u52a1\u4e0d\u53d7\u7b14\u8bb0\u672c\u4f11\u7720\u5f71\u54cd\uff0c\u7edf\u4e00FastQC\u7248\u672c\u548c\u76ee\u5f55\u7ed3\u6784\uff0c\u5e76\u6309\u6587\u4ef6\u6570\u4e0eI\/O\u80fd\u529b\u63a7\u5236\u5e76\u53d1\uff1b\u4e0d\u8981\u628a\u7ebf\u7a0b\u6570\u65e0\u9650\u5f00\u5927\uff0c\u5426\u5219\u591a\u4e2agzip\u89e3\u538b\u4efb\u52a1\u4f1a\u4e92\u76f8\u4e89\u62a2\u78c1\u76d8\u3002<\/p>\n
FastQC\u80fd\u62a5\u8b66\uff0c\u4f46\u4e0d\u80fd\u66ff\u4f60\u4e0b\u7ed3\u8bba<\/h2>\n
FastQC\u4e0d\u4f1a\u81ea\u52a8\u4fee\u526a\uff0c\u4e5f\u4e0d\u80fd\u8bc1\u660e\u6c61\u67d3\u6765\u6e90\uff1b\u8fc7\u8868\u8fbe\u5e8f\u5217\u7684\u6570\u636e\u5e93\u5339\u914d\u53ea\u662f\u65b9\u5411\u63d0\u793a\u3002\u5b83\u8fd8\u4e0d\u77e5\u9053\u4f60\u7684\u5efa\u5e93\u7b56\u7565\uff0c\u56e0\u6b64small RNA\u3001\u6269\u589e\u5b50\u3001RNA-seq\u3001ATAC-seq\u548c\u4e9a\u786b\u9178\u6c22\u76d0\u6d4b\u5e8f\u4e0d\u80fd\u7528\u540c\u4e00\u5957\u201c\u5168\u7eff\u201d\u6807\u51c6\u3002\u6700\u7ec8\u51b3\u5b9a\u5e94\u7ed3\u5408\u5b9e\u9a8c\u7c7b\u578b\u3001\u540c\u6279\u6837\u672c\u3001\u526a\u5207\u524d\u540e\u53d8\u5316\u4ee5\u53ca\u4e0b\u6e38\u6bd4\u5bf9\u7387\u3001\u63d2\u5165\u7247\u6bb5\u548c\u5b9a\u91cf\u8868\u73b0\u3002<\/p>\n
\u5982\u679cFastQC\u6279\u91cf\u62a5\u544a\u96be\u4ee5\u5224\u65ad\uff0c\u53ef\u4ee5\u51c6\u5907\uff1aFastQC\u7248\u672c\u3001\u6d4b\u5e8f\u7c7b\u578b\u3001\u6837\u672c\u6570\u4e0e\u6587\u4ef6\u5927\u5c0f\u3001\u4e00\u4e2a\u53ef\u5206\u4eab\u7684HTML\u6216\u6a21\u5757\u622a\u56fe\u3001\u8ba1\u5212\u7684\u4e0b\u6e38\u5206\u6790\u548c\u5b8c\u6210\u671f\u9650\u3002\u4e91\u751f\u4fe1\u53ef\u5148\u534f\u52a9\u5224\u65ad\u662f\u53c2\u6570\u4e0e\u6d41\u7a0b\u95ee\u9898\u3001\u9700\u8981\u7edf\u4e00\u670d\u52a1\u5668\u73af\u5883\uff0c\u8fd8\u662f\u9002\u5408\u5728\u7ea6\u5b9a\u8303\u56f4\u5185\u59d4\u6258\u5b8c\u6210\u6279\u91cf\u8d28\u63a7\u4e0e\u540e\u7eed\u5206\u6790\uff1b\u79df\u7528\u670d\u52a1\u5668\u81ea\u884c\u8fd0\u884c\u4e0e\u59d4\u6258\u5206\u6790\u7684\u8303\u56f4\u5206\u522b\u786e\u8ba4\u3002<\/p>\n
\u8054\u7cfb\u4e91\u751f\u4fe1\uff0c\u54a8\u8be2FastQC\u6279\u91cf\u8d28\u63a7\u4e0e\u5206\u6790\u73af\u5883<\/a><\/p>\n
\u53c2\u8003\u6765\u6e90<\/h2>\n