{"id":66422,"date":"2026-11-14T09:10:47","date_gmt":"2026-11-14T01:10:47","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66422"},"modified":"2026-11-14T09:10:47","modified_gmt":"2026-11-14T01:10:47","slug":"multiqc-missing-samples-name-collision-diagnosis","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/multiqc-missing-samples-name-collision-diagnosis\/","title":{"rendered":"MultiQC\u62a5\u544a\u4e3a\u4ec0\u4e48\u5c11\u4e86\u6837\u672c\uff1f\u5148\u67e5\u6837\u672c\u540d\u8986\u76d6\uff0c\u518d\u6000\u7591\u6d41\u7a0b\u5931\u8d25"},"content":{"rendered":"<div id=\"articleContent\" style=\"max-width:860px;margin:0 auto;color:#1f2937;font-size:17px;line-height:1.85;\">\n<p>\u5047\u8bbe\u8f93\u5165\u6e05\u5355\u670996\u4e2a\u6837\u672c\uff0cMultiQC\u603b\u8868\u91cc\u5374\u53ea\u526994\u4e2a\uff1b\u539f\u59cb\u548c\u526a\u5207\u540e\u7684FastQC\u7ed3\u679c\u653e\u5728\u4e00\u8d77\uff0c\u62a5\u544a\u91cc\u53c8\u50cf\u53ea\u8dd1\u4e86\u4e00\u8f6e\u3002\u9047\u5230\u8fd9\u79cd\u60c5\u51b5\uff0c\u5f88\u591a\u4eba\u4f1a\u91cd\u65b0\u8dd1\u4e0a\u6e38\u6d41\u7a0b\uff0c\u4f46\u95ee\u9898\u5f80\u5f80\u4e0d\u5728\u8ba1\u7b97\u5931\u8d25\uff0c\u800c\u5728MultiQC\u600e\u6837\u627e\u5230\u65e5\u5fd7\u3001\u600e\u6837\u6e05\u6d17\u6837\u672c\u540d\uff0c\u4ee5\u53ca\u540c\u540d\u7ed3\u679c\u600e\u6837\u5199\u5165\u62a5\u544a\u3002<\/p>\n<p>MultiQC\u4e0d\u662f\u8d28\u63a7\u7b97\u6cd5\uff0c\u4e5f\u4e0d\u4f1a\u91cd\u65b0\u5206\u6790FASTQ\u6216BAM\u3002\u5b83\u626b\u63cf\u5df2\u6709\u65e5\u5fd7\u548c\u7ed3\u679c\u6587\u4ef6\uff0c\u8c03\u7528\u5bf9\u5e94\u6a21\u5757\u89e3\u6790\u6307\u6807\uff0c\u518d\u6c47\u603b\u6210\u4e00\u4e2aHTML\u62a5\u544a\u3002\u8981\u5224\u65ad\u201c\u5c11\u6837\u672c\u201d\uff0c\u5fc5\u987b\u628a\u6587\u4ef6\u53d1\u73b0\u3001\u6a21\u5757\u8bc6\u522b\u3001\u6837\u672c\u547d\u540d\u548c\u62a5\u544a\u5c55\u793a\u5206\u5f00\u68c0\u67e5\u3002<\/p>\n<figure style=\"margin:28px 0;\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/daily-bio-blog-20261114-multiqc-missing-samples-multiqc-poster.png?ssl=1\" alt=\"MultiQC\u7f3a\u6837\u672c\u8bca\u65ad\u6d77\u62a5\uff1a\u4ece\u6587\u4ef6\u6e05\u5355\u3001\u6a21\u5757\u8bc6\u522b\u3001\u6837\u672c\u540d\u8986\u76d6\u5230\u62a5\u544a\u5c55\u793a\u9010\u6b65\u6392\u67e5\" style=\"display:block;width:100%;height:auto;border-radius:14px;\"><figcaption style=\"margin-top:8px;color:#64748b;font-size:14px;text-align:center;\">MultiQC\u7f3a\u6837\u672c\u8bca\u65ad\uff1a\u4f9d\u6b21\u6838\u5bf9\u6587\u4ef6\u53d1\u73b0\u3001\u6a21\u5757\u8bc6\u522b\u3001\u6837\u672c\u547d\u540d\u548c\u62a5\u544a\u5c55\u793a\u3002<\/figcaption><\/figure>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u5148\u6838\u5bf9\u7248\u672c\u3001\u8f93\u5165\u548c\u8f93\u51fa<\/h2>\n<p>\u672c\u6587\u4e8e2026\u5e7411\u670814\u65e5\u6838\u5bf9MultiQC\u5b98\u65b9\u8d44\u6599\uff0c\u5f53\u524d\u6700\u65b0\u53d1\u5e03\u7248\u4e3a1.35\uff0c\u8981\u6c42Python 3.9\u53ca\u4ee5\u4e0a\u3002\u5b98\u65b9\u652f\u6301\u5de5\u5177\u9875\u5f53\u65e5\u5217\u51fa182\u4e2a\u5de5\u5177\u6a21\u5757\uff0c\u4f46\u201c\u652f\u6301\u67d0\u5de5\u5177\u201d\u4e0d\u7b49\u4e8e\u5176\u6240\u6709\u5386\u53f2\u6216\u672a\u6765\u65e5\u5fd7\u683c\u5f0f\u90fd\u80fd\u8bc6\u522b\uff0c\u5347\u7ea7\u4e0a\u6e38\u8f6f\u4ef6\u540e\u4ecd\u5e94\u505a\u5c0f\u6279\u9a8c\u8bc1\u3002<\/p>\n<p>MultiQC\u7684\u8f93\u5165\u901a\u5e38\u4e0d\u662f\u539f\u59cb\u6d4b\u5e8f\u6587\u4ef6\uff0c\u800c\u662fFastQC ZIP\u3001STAR\u65e5\u5fd7\u3001samtools stats\u3001featureCounts summary\u7b49\u5de5\u5177\u8f93\u51fa\u3002\u6700\u7b80\u5355\u7684\u547d\u4ee4\u662f\uff1a<\/p>\n<pre style=\"overflow-x:auto;padding:18px;background:#102f47;color:#f8fafc;border-radius:12px;line-height:1.55;\"><code class=\"language-bash\">multiqc results\/ -o qc_summary -n project_qc.html -f -v\r\n<\/code><\/pre>\n<p><code>-o<\/code>\u6307\u5b9a\u8f93\u51fa\u76ee\u5f55\uff0c<code>-n<\/code>\u6307\u5b9a\u62a5\u544a\u540d\uff0c<code>-f<\/code>\u8986\u76d6\u540c\u540d\u65e7\u62a5\u544a\uff0c<code>-v<\/code>\u6253\u5370\u66f4\u8be6\u7ec6\u7684\u641c\u7d22\u4e0e\u89e3\u6790\u65e5\u5fd7\u3002\u6b63\u5e38\u8f93\u51fa\u81f3\u5c11\u5305\u62ecHTML\u548c<code>multiqc_data\/<\/code>\uff1b\u540e\u8005\u4fdd\u5b58\u53ef\u4f9b\u4e0b\u6e38\u590d\u6838\u7684\u8868\u683c\u6570\u636e\u3002\u5b98\u65b9\u6587\u6863\u8fd8\u8bf4\u660e\uff0c1.29\u53ca\u4ee5\u4e0a\u7248\u672c\u4f1a\u5728\u6570\u636e\u76ee\u5f55\u751f\u6210<code>multiqc.parquet<\/code>\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u5b98\u65b9\u793a\u4f8b\u63ed\u793a\u4e86\u6700\u9690\u853d\u7684\u95ee\u9898\uff1a\u540c\u540d\u8986\u76d6<\/h2>\n<p>\u5b98\u65b9\u6587\u6863\u7ed9\u51fa\u4e09\u4e2a\u76ee\u5f55\uff1a<code>analysis_1<\/code>\u3001<code>analysis_2<\/code>\u3001<code>analysis_3<\/code>\uff0c\u6bcf\u4e2a\u76ee\u5f55\u90fd\u6709\u540d\u4e3a<code>sample_1.fastq.gz.aligned.log<\/code>\u7684\u6587\u4ef6\u3002MultiQC\u6e05\u6d17\u6587\u4ef6\u540d\u540e\uff0c\u4e09\u4efd\u7ed3\u679c\u90fd\u53ef\u80fd\u53d8\u6210<code>sample_1<\/code>\u3002\u5982\u679c\u65e5\u5fd7\u89e3\u6790\u5f97\u5230\u76f8\u540c\u6837\u672c\u540d\uff0c\u5148\u524d\u6570\u636e\u4f1a\u88ab\u540e\u6765\u7684\u6570\u636e\u8986\u76d6\uff1b\u547d\u4ee4\u6210\u529f\u3001HTML\u4e5f\u80fd\u6253\u5f00\uff0c\u4f46\u6837\u672c\u6570\u5df2\u7ecf\u53d8\u5c11\u3002<\/p>\n<p>\u5148\u7528\u8be6\u7ec6\u65e5\u5fd7\u786e\u8ba4\u8986\u76d6\uff0c\u518d\u4e3a\u6837\u672c\u540d\u52a0\u76ee\u5f55\u524d\u7f00\uff1a<\/p>\n<pre style=\"overflow-x:auto;padding:18px;background:#102f47;color:#f8fafc;border-radius:12px;line-height:1.55;\"><code class=\"language-bash\">multiqc -v -d -dd 1 results\/ -o qc_summary -f\r\n<\/code><\/pre>\n<p><code>-d<\/code>\u628a\u76ee\u5f55\u52a0\u5165\u6837\u672c\u540d\uff0c<code>-dd 1<\/code>\u53ea\u53d6\u8def\u5f84\u672b\u7aef\u4e00\u4e2a\u76ee\u5f55\u3002\u82e5\u51b2\u7a81\u6765\u81ea\u6587\u4ef6\u540d\u88ab\u8fc7\u5ea6\u622a\u65ad\uff0c\u53ef\u7528<code>-s\/--fullnames<\/code>\u4e34\u65f6\u5173\u95ed\u6837\u672c\u540d\u6e05\u6d17\u3002\u53ea\u6709\u5f53\u65e5\u5fd7\u5185\u90e8\u8bb0\u5f55\u7684\u6837\u672c\u540d\u65e0\u6548\u65f6\uff0c\u624d\u8003\u8651<code>--fn_as_s_name<\/code>\u5f3a\u5236\u91c7\u7528\u65e5\u5fd7\u6587\u4ef6\u540d\uff1b\u8fd9\u4e2a\u9009\u9879\u4f1a\u5f71\u54cd\u6a21\u5757\u8bc6\u522b\u540e\u7684\u547d\u540d\uff0c\u4e0d\u80fd\u4e0d\u770b\u7ed3\u679c\u5c31\u5168\u5c40\u5957\u7528\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u516d\u79cd\u201c\u5c11\u6837\u672c\u201d\u73b0\u8c61\uff0c\u5904\u7406\u65b9\u5f0f\u4e0d\u540c<\/h2>\n<div style=\"overflow-x:auto;margin:18px 0 26px;\">\n<table style=\"width:100%;border-collapse:collapse;min-width:720px;font-size:15px;\">\n<thead style=\"background:#176b87;color:#fff;\">\n<tr>\n<th style=\"padding:12px;border:1px solid #d7e5ea;text-align:left;\">\u73b0\u8c61<\/th>\n<th style=\"padding:12px;border:1px solid #d7e5ea;text-align:left;\">\u66f4\u53ef\u80fd\u7684\u539f\u56e0<\/th>\n<th style=\"padding:12px;border:1px solid #d7e5ea;text-align:left;\">\u5148\u505a\u7684\u68c0\u67e5<\/th>\n<th style=\"padding:12px;border:1px solid #d7e5ea;text-align:left;\">\u5408\u9002\u5904\u7406<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u6574\u4e2a\u5de5\u5177\u6a21\u5757\u90fd\u6ca1\u6709<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u6587\u4ef6\u683c\u5f0f\u6216\u641c\u7d22\u6a21\u5f0f\u672a\u88ab\u8bc6\u522b<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u770b<code>-v<\/code>\u4e2d\u627e\u5230\u54ea\u4e9b\u6587\u4ef6\u3001\u89e6\u53d1\u54ea\u4e2a\u6a21\u5757<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u6838\u5bf9\u4e0a\u6e38\u7248\u672c\u4e0e\u6a21\u5757\u6587\u6863\uff0c\u5148\u62ff1\u20142\u4efd\u65e5\u5fd7\u8bd5\u8dd1<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u6837\u672c\u6570\u5c11\u4e8e\u6587\u4ef6\u6570<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u6e05\u6d17\u540e\u6837\u672c\u540d\u76f8\u540c\uff0c\u540e\u8bfb\u7ed3\u679c\u8986\u76d6\u524d\u8005<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u641c\u7d22verbose\u65e5\u5fd7\u4e2d\u7684\u8986\u76d6\u63d0\u793a\uff0c\u6bd4\u8f83\u539f\u540d\u4e0e\u663e\u793a\u540d<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u7528<code>-d -dd 1<\/code>\u4fdd\u7559\u6279\u6b21\u76ee\u5f55\uff0c\u6216\u8c03\u6574\u547d\u540d\u89c4\u5219<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u539f\u59cb\u4e0e\u526a\u5207\u540e\u7ed3\u679c\u6df7\u5728\u4e00\u8d77<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u4e24\u4e2a\u9636\u6bb5\u6e05\u6d17\u6210\u540c\u4e00\u540d\u5b57<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u5206\u522b\u7edf\u8ba1raw\u3001trimmed\u6587\u4ef6\u6e05\u5355<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u5206\u76ee\u5f55\u51fa\u62a5\u544a\uff0c\u6216\u7528\u914d\u7f6e\u533a\u5206\u6a21\u5757\u5b9e\u4f8b<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u51fa\u73b0\u4e0d\u8be5\u6709\u7684\u65e7\u6837\u672c<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u9012\u5f52\u626b\u63cf\u5230\u5386\u53f2\u76ee\u5f55\u3001\u4e34\u65f6\u65e5\u5fd7<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u8bb0\u5f55\u5b9e\u9645\u626b\u63cf\u8def\u5f84\u548c\u6587\u4ef6\u6e05\u5355<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u7528<code>--file-list<\/code>\u9650\u5b9a\u8f93\u5165\uff0c\u6216\u7528<code>--ignore<\/code>\u6392\u9664<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u6307\u5b9a\u6a21\u5757\u7f3a\u5931\u4f46\u547d\u4ee4\u4ecd\u6210\u529f<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u9ed8\u8ba4\u4f1a\u7ee7\u7eed\u5904\u7406\u5176\u4ed6\u5df2\u8bc6\u522b\u6a21\u5757<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u6838\u5bf9\u9884\u671f\u6a21\u5757\u662f\u5426\u771f\u7684\u51fa\u73b0\u5728\u62a5\u544a<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u914d\u5408<code>-m<\/code>\u4f7f\u7528<code>--require-logs<\/code>\uff0c\u7f3a\u5931\u65f6\u9000\u51fa\u7801\u4e3a1<\/td>\n<\/tr>\n<tr>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">HTML\u6253\u5f00\u540e\u5361\u987f<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u6837\u672c\u591a\u3001\u4ea4\u4e92\u56fe\u548c\u5927\u8868\u6e32\u67d3\u538b\u529b\u9ad8<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u533a\u5206\u751f\u6210\u8017\u65f6\u4e0e\u6d4f\u89c8\u5668\u6e32\u67d3\u8017\u65f6<\/td>\n<td style=\"padding:11px;border:1px solid #d7e5ea;vertical-align:top;\">\u7b5b\u9009\u8303\u56f4\u3001\u4f7f\u7528flat\u56fe\uff0c\u4e0d\u8981\u5f3a\u5236\u6240\u6709\u56fe\u4ea4\u4e92\u5316<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u4e0d\u8981\u53ea\u7528\u201cHTML\u80fd\u6253\u5f00\u201d\u5224\u65ad\u6210\u529f<\/h2>\n<p>\u4e00\u5957\u53ef\u590d\u7528\u7684\u9a8c\u6536\u987a\u5e8f\u662f\uff1a\u5148\u751f\u6210\u9884\u671f\u8f93\u5165\u6e05\u5355\uff0c\u6309\u5de5\u5177\u6216\u9636\u6bb5\u7edf\u8ba1\u6587\u4ef6\u6570\uff1b\u518d\u4fdd\u5b58MultiQC\u7248\u672c\u3001\u5b8c\u6574\u547d\u4ee4\u548cverbose\u65e5\u5fd7\uff1b\u968f\u540e\u6838\u5bf9\u62a5\u544a\u6a21\u5757\u3001\u6837\u672c\u6570\u548c\u6837\u672c\u540d\uff1b\u6700\u540e\u62bd\u67e52\u20143\u4e2a\u5f02\u5e38\u6837\u672c\uff0c\u628aMultiQC\u4e2d\u7684\u6570\u503c\u4e0e\u539f\u59cb\u65e5\u5fd7\u5bf9\u56de\u53bb\u3002<\/p>\n<p>\u5982\u679c\u6d41\u6c34\u7ebf\u660e\u786e\u8981\u6c42FastQC\u548cSTAR\u90fd\u5b58\u5728\uff0c\u53ef\u4ee5\u8ba9\u7f3a\u5931\u76f4\u63a5\u5931\u8d25\uff1a<\/p>\n<pre style=\"overflow-x:auto;padding:18px;background:#102f47;color:#f8fafc;border-radius:12px;line-height:1.55;\"><code class=\"language-bash\">multiqc results\/ -m fastqc -m star --require-logs \\\r\n  --file-list qc_inputs.txt -o qc_summary -f\r\n<\/code><\/pre>\n<p><code>--file-list<\/code>\u6bcf\u884c\u4e00\u4e2a\u5f85\u641c\u7d22\u6587\u4ef6\u8def\u5f84\uff0c\u80fd\u907f\u514d\u9879\u76ee\u6839\u76ee\u5f55\u91cc\u65e7\u7ed3\u679c\u88ab\u610f\u5916\u626b\u5165\u3002\u9700\u8981\u6ce8\u610f\uff1a\u6587\u4ef6\u6570\u91cf\u4e0d\u4e00\u5b9a\u7b49\u4e8e\u6837\u672c\u6570\u91cf\uff0c\u4e00\u4e2a\u6837\u672c\u53ef\u80fd\u6709R1\/R2\u3001\u591a\u4e2alane\u6216\u591a\u4e2a\u5de5\u5177\u65e5\u5fd7\uff0c\u6240\u4ee5\u5e94\u6309\u6a21\u5757\u548c\u5efa\u5e93\u8bbe\u8ba1\u5236\u5b9a\u9884\u671f\uff0c\u800c\u4e0d\u662f\u673a\u68b0\u6bd4\u8f83\u603b\u6587\u4ef6\u6570\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u5927\u62a5\u544a\u7684\u201c\u53d8\u5316\u201d\u53ef\u80fd\u53ea\u662f\u5c55\u793a\u7b56\u7565<\/h2>\n<p>MultiQC\u5b98\u65b9\u4e3a\u5927\u6837\u672c\u62a5\u544a\u8bbe\u7f6e\u4e86\u81ea\u52a8\u964d\u8f7d\u89c4\u5219\uff1a\u56fe\u4e2d\u6837\u672c\u8fbe\u523050\u4e2a\u65f6\uff0c\u90e8\u5206\u56fe\u9ed8\u8ba4\u4e0d\u5728\u52a0\u8f7d\u65f6\u7acb\u5373\u7ed8\u5236\uff1b\u8868\u683c\u8fbe\u5230500\u884c\u65f6\u53ef\u8f6c\u4e3a\u5c0f\u63d0\u7434\u56fe\uff1b\u56fe\u4e2d\u5e8f\u5217\u8fbe\u52301000\u6761\u65f6\u9ed8\u8ba4\u751f\u6210flat\u56fe\uff1b\u8d85\u8fc72000\u4e2a\u6837\u672c\u65f6\uff0c\u5c0f\u63d0\u7434\u56fe\u6570\u636e\u8fd8\u4f1a\u4e0b\u91c7\u6837\u3002\u8fd9\u4e9b\u6570\u5b57\u662f\u62a5\u544a\u5c55\u793a\u9608\u503c\uff0c\u4e0d\u662f\u6570\u636e\u8d28\u63a7\u9608\u503c\uff0c\u4e5f\u4e0d\u8868\u793a\u6837\u672c\u88ab\u5220\u9664\u3002<\/p>\n<p>\u56e0\u6b64\uff0c\u770b\u5230\u7070\u8272\u201cShow Plot\u201d\u3001\u5c0f\u63d0\u7434\u56fe\u6216\u9759\u6001\u56fe\uff0c\u5148\u68c0\u67e5\u754c\u9762\u8bbe\u7f6e\u548c<code>multiqc_data\/<\/code>\uff0c\u4e0d\u8981\u628a\u663e\u793a\u65b9\u5f0f\u6539\u53d8\u8bef\u5224\u4e3a\u5206\u6790\u5931\u8d25\u3002\u5b98\u65b9\u4e5f\u660e\u786e\u63d0\u9192\uff0cPDF\u4f1a\u5173\u95ed\u8bb8\u591a\u4ea4\u4e92\u529f\u80fd\uff0c\u751a\u81f3\u7f3a\u5c11\u90e8\u5206\u56fe\uff1b\u4ea4\u4ed8\u65f6\u4f18\u5148\u4fdd\u7559HTML\u548c\u6570\u636e\u76ee\u5f55\uff0cPDF\u53ea\u4f5c\u8865\u5145\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u672c\u5730\u591f\u4e0d\u591f\uff0c\u53d6\u51b3\u4e8e\u65e5\u5fd7\u89c4\u6a21\u548c\u6570\u636e\u4f4d\u7f6e<\/h2>\n<p>\u51e0\u5341\u5230\u51e0\u767e\u4efd\u65e5\u5fd7\u3001\u76ee\u5f55\u7ed3\u6784\u6e05\u695a\u65f6\uff0cMultiQC\u901a\u5e38\u53ef\u5728\u672c\u5730\u5b8c\u6210\uff0c\u5b83\u4e0d\u9700\u8981GPU\u3002\u5148\u9650\u5b9a\u641c\u7d22\u8def\u5f84\u3001\u89e3\u51b3\u547d\u540d\u51b2\u7a81\uff0c\u6bd4\u589e\u52a0\u786c\u4ef6\u66f4\u6709\u6548\u3002\u771f\u6b63\u503c\u5f97\u628a\u4efb\u52a1\u653e\u5230\u670d\u52a1\u5668\u4e0a\u7684\u573a\u666f\uff0c\u662f\u4e0a\u6e38\u6d41\u7a0b\u672c\u6765\u5c31\u5728\u670d\u52a1\u5668\u3001\u65e5\u5fd7\u5206\u6563\u5728\u591a\u4e2a\u6279\u6b21\u3001\u9700\u8981\u5b9a\u65f6\u91cd\u8dd1\u5e76\u4fdd\u7559\u7248\u672c\u5316\u62a5\u544a\uff0c\u6216\u6709\u6570\u5343\u6837\u672c\u5bfc\u81f4\u626b\u63cf\u3001\u62a5\u544a\u751f\u6210\u548c\u6d4f\u89c8\u5668\u6253\u5f00\u90fd\u9700\u8981\u7a33\u5b9a\u73af\u5883\u3002<\/p>\n<p>\u670d\u52a1\u5668\u7684\u4ef7\u503c\u662f\u8ba9\u6c47\u603b\u4efb\u52a1\u9760\u8fd1\u6570\u636e\uff0c\u56fa\u5b9aMultiQC\u7248\u672c\u3001\u914d\u7f6e\u6587\u4ef6\u548c\u8f93\u5165\u6e05\u5355\uff0c\u5e76\u628a\u62a5\u544a\u7eb3\u5165\u6d41\u6c34\u7ebf\uff1b\u5b83\u4e0d\u4f1a\u66ff\u4ee3\u5bf9\u5f02\u5e38\u6307\u6807\u7684\u751f\u7269\u5b66\u5224\u65ad\u3002\u73af\u5883\u90e8\u7f72\u3001\u79df\u7528\u670d\u52a1\u5668\u81ea\u884c\u8fd0\u884c\u548c\u59d4\u6258\u6279\u91cf\u8d28\u63a7\u5206\u6790\uff0c\u5e94\u5206\u522b\u786e\u8ba4\u8303\u56f4\u3002<\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">MultiQC\u80fd\u6c47\u603b\u8bc1\u636e\uff0c\u4e0d\u80fd\u7ed9\u6570\u636e\u5224\u751f\u6b7b<\/h2>\n<p>MultiQC\u628a\u591a\u4e2a\u5de5\u5177\u7684\u8f93\u51fa\u653e\u5728\u540c\u4e00\u89c6\u56fe\uff0c\u4f46\u4e0d\u4f1a\u81ea\u52a8\u8bc1\u660e\u6837\u672c\u5408\u683c\uff0c\u4e5f\u4e0d\u80fd\u5f25\u8865\u4e0a\u6e38\u65e5\u5fd7\u7f3a\u5931\u3002\u6a21\u5757\u4e4b\u95f4\u7684\u7ea2\u9ec4\u989c\u8272\u3001\u9608\u503c\u548c\u5355\u4f4d\u4ecd\u6765\u81ea\u5404\u81ea\u5de5\u5177\u3002\u9047\u5230\u7f3a\u6837\u672c\uff0c\u5148\u56de\u7b54\u201c\u6587\u4ef6\u6709\u6ca1\u6709\u88ab\u627e\u5230\u3001\u6a21\u5757\u6709\u6ca1\u6709\u8bc6\u522b\u3001\u540d\u5b57\u6709\u6ca1\u6709\u51b2\u7a81\u3001\u5c55\u793a\u6709\u6ca1\u6709\u964d\u8f7d\u201d\uff0c\u518d\u51b3\u5b9a\u662f\u5426\u91cd\u8dd1\u4e0a\u6e38\u3002<\/p>\n<p>\u5982\u679c\u62a5\u544a\u6837\u672c\u6570\u5bf9\u4e0d\u4e0a\uff0c\u53ef\u4ee5\u51c6\u5907MultiQC\u7248\u672c\u3001\u76ee\u5f55\u6811\u3001\u9884\u671f\u4e0e\u5b9e\u9645\u6837\u672c\u6570\u3001\u8fd0\u884c\u547d\u4ee4\u548c\u4e00\u6bb5\u53ef\u5206\u4eab\u7684verbose\u65e5\u5fd7\u3002\u4e91\u751f\u4fe1\u53ef\u5148\u534f\u52a9\u5224\u65ad\u662f\u547d\u540d\u4e0e\u914d\u7f6e\u95ee\u9898\u3001\u9700\u8981\u7edf\u4e00\u670d\u52a1\u5668\u73af\u5883\uff0c\u8fd8\u662f\u9002\u5408\u5728\u7ea6\u5b9a\u8303\u56f4\u5185\u59d4\u6258\u5b8c\u6210\u6279\u91cf\u8d28\u63a7\u4e0e\u540e\u7eed\u5206\u6790\u3002<\/p>\n<p><a href=\"https:\/\/work.weixin.qq.com\/kfid\/kfcb05492f29d4d6064\" data-ysx-event=\"contact_click\" style=\"display:inline-block;padding:13px 20px;border-radius:10px;background:#ef7d32;color:#fff;text-decoration:none;font-weight:800;\">\u8054\u7cfb\u4e91\u751f\u4fe1\uff0c\u54a8\u8be2MultiQC\u6279\u91cf\u8d28\u63a7\u4e0e\u73af\u5883\u914d\u7f6e<\/a><\/p>\n<h2 style=\"margin-top:34px;color:#12314a;\">\u53c2\u8003\u6765\u6e90<\/h2>\n<ul>\n<li><a href=\"https:\/\/github.com\/MultiQC\/MultiQC\/releases\/tag\/v1.35\">MultiQC 1.35\u5b98\u65b9\u53d1\u5e03\u8bf4\u660e<\/a><\/li>\n<li><a href=\"https:\/\/docs.seqera.io\/multiqc\/getting_started\/running_multiqc\">MultiQC\u5b98\u65b9\u8fd0\u884c\u4e0e\u547d\u4ee4\u884c\u6587\u6863<\/a><\/li>\n<li><a href=\"https:\/\/docs.seqera.io\/multiqc\/getting_started\/config\">MultiQC\u5b98\u65b9\u914d\u7f6e\u4e0e\u6837\u672c\u547d\u540d\u6587\u6863<\/a><\/li>\n<li><a href=\"https:\/\/docs.seqera.io\/multiqc\/modules\/\">MultiQC\u5b98\u65b9\u652f\u6301\u5de5\u5177\u5217\u8868<\/a><\/li>\n<li><a href=\"https:\/\/docs.seqera.io\/multiqc\/getting_started\/installation\">MultiQC\u5b98\u65b9\u5b89\u88c5\u6587\u6863<\/a><\/li>\n<\/ul>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>MultiQC\u62a5\u544a\u5c11\u6837\u672c\uff0c\u4e0d\u4e00\u5b9a\u662f\u4e0a\u6e38\u6d41\u7a0b\u5931\u8d25\u3002\u672c\u6587\u4ece\u6587\u4ef6\u53d1\u73b0\u3001\u6a21\u5757\u8bc6\u522b\u3001\u6837\u672c\u540d\u8986\u76d6\u548c\u5927\u62a5\u544a\u5c55\u793a\u56db\u5c42\u6392\u67e5\uff0c\u5e76\u7ed9\u51fa\u53ef\u590d\u7528\u7684\u547d\u4ee4\u4e0e\u9a8c\u6536\u8868\u3002<\/p>\n","protected":false},"author":1,"featured_media":66421,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[1,75],"tags":[527,532,80,539,541,540,528,110],"class_list":["post-66422","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-part","category-compute-environment","tag-fastqc","tag-multiqc","tag-rna-seq","tag-539","tag-541","tag-540","tag-528","tag-110"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/daily-bio-blog-20261114-multiqc-missing-samples-multiqc-poster.png?fit=1080%2C1350&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66422","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=66422"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66422\/revisions"}],"predecessor-version":[{"id":66423,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66422\/revisions\/66423"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/66421"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=66422"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=66422"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=66422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}