{"id":66471,"date":"2026-11-15T18:00:00","date_gmt":"2026-11-15T10:00:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66471"},"modified":"2026-11-15T14:01:10","modified_gmt":"2026-11-15T06:01:10","slug":"rental-q008-b","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/rental-q008-b\/","title":{"rendered":"\u5b8c\u6210\u4e00\u6b21\u77ed\u671f\u670d\u52a1\u5668\u5f00\u901a\u3001\u9a8c\u6536\u548c\u5230\u671f\u4ea4\u63a5\u6f14\u7ec3"},"content":{"rendered":"

<\/p>\n

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\"\u77ed\u671f\u670d\u52a1\u5668\uff0c\u5148\u505a\u4e00\u6b21\u5b8c\u6574\u4ea4\u63a5\u6f14\u7ec3\uff1a\u6838\u5bf9\u8d26\u53f7\u4e0e\u76ee\u5f55\uff1b\u8fd0\u884c\u5df2\u77e5\u7b54\u6848\u7684\u6837\u4f8b\uff1b\u4fdd\u5b58\u65e5\u5fd7\u548c\u6587\u4ef6\u6821\u9a8c\uff1b\u53d6\u56de\u7ed3\u679c\u518d\u590d\u6838\"
\u672c\u6587\u64cd\u4f5c\u4e0e\u68c0\u67e5\u8def\u5f84\u793a\u610f\u3002<\/figcaption><\/figure>\n

\u7b2c\u4e00\u6b21\u77ed\u671f\u79df\u7528\u670d\u52a1\u5668\uff0c\u53ef\u4ee5\u5148\u5b8c\u6210\u4e00\u6b21\u5c0f\u578b\u4ea4\u63a5\u6f14\u7ec3\uff1a\u767b\u5f55\u3001\u68c0\u67e5\u8d44\u6e90\u3001\u653e\u5165\u6837\u4f8b\u3001\u8fd0\u884c\u4efb\u52a1\u3001\u53d6\u56de\u7ed3\u679c\u3001\u9a8c\u8bc1\u4ea4\u4ed8\u3002\u6f14\u7ec3\u7684\u76ee\u6807\u662f\u66b4\u9732\u8def\u5f84\u3001\u6743\u9650\u3001\u73af\u5883\u548c\u4f20\u8f93\u95ee\u9898\uff0c\u907f\u514d\u628a\u6b63\u5f0f\u6570\u636e\u4e0a\u4f20\u540e\u624d\u53d1\u73b0\u4e0d\u80fd\u8fd0\u884c\u3002\u672c\u6587\u7ed9\u51fa\u4e00\u4e2a\u4e0d\u9700\u8981\u771f\u5b9e\u79d1\u7814\u6570\u636e\u7684\u64cd\u4f5c\u6837\u4f8b\uff0c\u518d\u8bf4\u660e\u600e\u6837\u628a\u5b83\u66ff\u6362\u6210\u9879\u76ee\u81ea\u5df1\u7684\u9a8c\u6536\u6d41\u7a0b\u3002<\/p>\n

\u5f00\u901a\u524d\u51c6\u5907\u4e00\u5f20\u9a8c\u6536\u6e05\u5355<\/h2>\n

\u7531\u79df\u7528\u65b9\u4e0e\u63d0\u4f9b\u65b9\u786e\u8ba4\u8d26\u53f7\u3001\u767b\u5f55\u65b9\u5f0f\u3001\u53ef\u7528CPU\u4e0e\u5185\u5b58\u3001\u5de5\u4f5c\u76d8\u8def\u5f84\u3001\u73af\u5883\u7248\u672c\u3001\u4f7f\u7528\u671f\u9650\u548c\u6280\u672f\u652f\u6301\u8303\u56f4\u3002\u5148\u62ff\u5230\u771f\u5b9e\u670d\u52a1\u5668\u5730\u5740\u3001\u8d26\u53f7\u548c\u53d7\u4fe1\u4efb\u7684\u4e3b\u673a\u6307\u7eb9\uff0c\u518d\u767b\u5f55\uff1b\u4e0d\u8981\u4e3a\u4e86\u8fde\u63a5\u65b9\u4fbf\u5173\u95edSSH\u4e3b\u673a\u8eab\u4efd\u6838\u9a8c\uff0c\u4e5f\u4e0d\u8981\u628a\u5bc6\u7801\u6216\u5bc6\u94a5\u5199\u8fdb\u9879\u76ee\u811a\u672c\u3002\u4ee5\u4e0b\u8def\u5f84\u4e0e\u4e3b\u673a\u540d\u90fd\u662f\u793a\u4f8b\uff0c\u5e94\u6309\u5df2\u786e\u8ba4\u7684\u8d44\u6599\u66ff\u6362\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
\u73af\u8282<\/th>\n\u6700\u5c0f\u68c0\u67e5<\/th>\n\u5408\u683c\u8bc1\u636e<\/th>\n<\/tr>\n<\/thead>\n
\u767b\u5f55<\/td>\n\u8d26\u53f7\u3001\u4e3b\u673a\u8eab\u4efd\u548c\u8bbf\u95ee\u8def\u5f84<\/td>\n\u80fd\u6309\u786e\u8ba4\u65b9\u5f0f\u767b\u5f55\u4e14\u8eab\u4efd\u4e00\u81f4<\/td>\n<\/tr>\n
\u76ee\u5f55<\/td>\n\u5de5\u4f5c\u76d8\u53ef\u5199\u3001\u7a7a\u95f4\u4e0e\u914d\u989d\u9002\u7528<\/td>\n\u6307\u5b9a\u76ee\u5f55\u53ef\u5efa\u7acb\u6587\u4ef6\u5e76\u8bfb\u53d6<\/td>\n<\/tr>\n
\u73af\u5883<\/td>\nPython\u6216\u76ee\u6807\u5de5\u5177\u7248\u672c<\/td>\n\u547d\u4ee4\u5b58\u5728\u4e14\u6837\u4f8b\u8f93\u51fa\u7b26\u5408\u9884\u671f<\/td>\n<\/tr>\n
\u7ed3\u679c<\/td>\n\u6587\u4ef6\u3001\u65e5\u5fd7\u3001\u9000\u51fa\u7801<\/td>\n\u63a5\u6536\u65b9\u80fd\u6821\u9a8c\u5e76\u6253\u5f00<\/td>\n<\/tr>\n
\u7ed3\u675f<\/td>\n\u7eed\u79df\u3001\u8fc1\u51fa\u3001\u4fdd\u7559\u89c4\u5219<\/td>\n\u4ea4\u63a5\u6e05\u5355\u5b8c\u6210\uff0c\u672a\u64c5\u81ea\u5220\u9664<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5728\u72ec\u7acb\u76ee\u5f55\u521b\u5efa\u4e00\u4e2a\u6559\u5b66\u6837\u4f8b<\/h2>\n

\u4e0b\u9762\u7684\u4e09\u6761\u77ed\u5e8f\u5217\u53ea\u662f\u6559\u5b66\u6570\u636e\uff0c\u4e0d\u662f\u5b9e\u9a8c\u6837\u672c\uff0c\u4e5f\u4e0d\u80fd\u7528\u4e8e\u8bc4\u4ef7\u670d\u52a1\u5668\u7684\u5206\u6790\u6027\u80fd\u3002\u547d\u4ee4\u53ea\u5728\u65b0\u5efa\u7684\u6f14\u7ec3\u76ee\u5f55\u5199\u6587\u4ef6\uff0c\u4e0d\u4fee\u6539\u7cfb\u7edf\u73af\u5883\u3002\u82e5\u76ee\u6807\u5de5\u4f5c\u9700\u8981R\u3001\u5bb9\u5668\u6216\u5176\u4ed6\u8f6f\u4ef6\uff0c\u8fd8\u8981\u4e3a\u8be5\u5de5\u5177\u53e6\u5916\u5b89\u6392\u9a8c\u6536\uff0c\u4e0d\u80fd\u4ee5Python\u80fd\u8fd0\u884c\u66ff\u4ee3\u6240\u6709\u73af\u5883\u9a8c\u6536\u3002<\/p>\n

mkdir -p "$HOME\/rental-demo"\/{input,results,logs}\ncd "$HOME\/rental-demo"\npython3 --version > logs\/python-version.txt\nprintf '>demo_1\\nACGTACGT\\n>demo_2\\nAACCGGTT\\n>demo_3\\nATGC\\n' > input\/demo.fa\nsha256sum input\/demo.fa > input.sha256\nsha256sum -c input.sha256<\/code><\/pre>\n
\u8fd0\u884c\u4e00\u4e2a\u6709\u660e\u786e\u9884\u671f\u7684\u6700\u5c0f\u4efb\u52a1<\/h2>\n
\u5c06\u4e0b\u5217\u7a0b\u5e8f\u4fdd\u5b58\u6210count_fasta.py\uff0c\u518d\u8fd0\u884c\u3002\u5b83\u53ea\u5bf9\u8fd9\u4e2a\u53d7\u63a7\u6837\u4f8b\u7edf\u8ba1\u5e8f\u5217\u6761\u6570\u548c\u78b1\u57fa\u603b\u6570\uff0c\u4e0d\u662f\u901a\u7528FASTA\u8d28\u91cf\u63a7\u5236\u5de5\u5177\u3002\u9884\u671f\u503c\u662f3\u6761\u5e8f\u5217\u300120\u4e2a\u5b57\u7b26\u3002\u901a\u8fc7\u8fd9\u79cd\u6709\u5df2\u77e5\u7b54\u6848\u7684\u4efb\u52a1\uff0c\u53ef\u4ee5\u533a\u5206\u201c\u547d\u4ee4\u6210\u529f\u9000\u51fa\u201d\u548c\u201c\u8f93\u51fa\u771f\u6b63\u7b26\u5408\u7ea6\u5b9a\u201d\u3002<\/p>\n
from pathlib import Path\nrecords = 0\nbases = 0\nfor line in Path("input\/demo.fa").read_text().splitlines():\n    if line.startswith(">"):\n        records += 1\n    else:\n        bases += len(line.strip())\nPath("results\/counts.tsv").write_text(\n    f"records\\tbases\\n{records}\\t{bases}\\n"\n)<\/code><\/pre>\n
python3 count_fasta.py > logs\/run.stdout.txt 2> logs\/run.stderr.txt\nstatus=$?\nprintf 'exit_status=%s\\n' "$status" > logs\/run.exit.txt\ncat results\/counts.tsv\nsha256sum results\/counts.tsv logs\/python-version.txt > outputs.sha256\nsha256sum -c outputs.sha256<\/code><\/pre>\n
\u82e5\u9000\u51fa\u7801\u975e\u96f6\uff0c\u5148\u67e5\u770b\u9519\u8bef\u65e5\u5fd7\uff1b\u627e\u4e0d\u5230\u8f93\u5165\u8981\u68c0\u67e5\u5f53\u524d\u76ee\u5f55\u4e0e\u6587\u4ef6\u540d\uff0cPermission denied\u8981\u6838\u5bf9\u8d26\u53f7\u548c\u76ee\u5f55\u6743\u9650\uff0cNo space left\u8981\u6838\u5bf9\u6302\u8f7d\u70b9\u3001\u914d\u989d\u53cainode\u3002\u4e0d\u8981\u9047\u5230\u9519\u8bef\u5c31\u76f4\u63a5\u6362\u66f4\u5927\u914d\u7f6e\uff0c\u8fd9\u4e2a\u5c0f\u4efb\u52a1\u51e0\u4e4e\u4e0d\u6784\u6210\u7b97\u529b\u9009\u578b\u8bc1\u636e\u3002<\/p>\n
\u628a\u7ed3\u679c\u53d6\u56de\u5230\u7b2c\u4e8c\u4e2a\u4f4d\u7f6e\uff0c\u518d\u6821\u9a8c<\/h2>\n
\u5728\u63a5\u6536\u7aef\u521b\u5efa\u72ec\u7acb\u76ee\u5f55\uff0c\u901a\u8fc7\u5df2\u6838\u9a8c\u7684SSH\u8fde\u63a5\u590d\u5236\u6f14\u7ec3\u7ed3\u679c\u3002rsync\u6e90\u76ee\u5f55\u672b\u5c3e\u7684\u659c\u6760\u8868\u793a\u590d\u5236\u76ee\u5f55\u5185\u5bb9\uff1b\u6b63\u5f0f\u64cd\u4f5c\u524d\u5148\u786e\u8ba4\u76ee\u6807\u4f4d\u7f6e\uff0c\u672c\u6587\u4e0d\u4f7f\u7528\u5220\u9664\u76ee\u6807\u6587\u4ef6\u7684\u540c\u6b65\u9009\u9879\u3002\u4f20\u8f93\u5b8c\u6210\u540e\uff0c\u5728\u63a5\u6536\u76ee\u5f55\u91cd\u65b0\u6267\u884c\u6821\u9a8c\uff0c\u786e\u4fdd\u6821\u9a8c\u6e05\u5355\u4e0e\u6587\u4ef6\u76f8\u5bf9\u8def\u5f84\u4fdd\u6301\u5bf9\u5e94\u3002<\/p>\n
# \u5728\u63a5\u6536\u7aef\u6267\u884c\uff1b\u66ff\u6362\u8d26\u53f7\u3001\u4e3b\u673a\u548c\u76ee\u5f55\nmkdir -p received-demo\nrsync -av --partial researcher@server.example.org:rental-demo\/ received-demo\/\ncd received-demo\nsha256sum -c input.sha256\nsha256sum -c outputs.sha256<\/code><\/pre>\n
\u7528\u771f\u5b9e\u9879\u76ee\u7684\u6837\u4f8b\u66ff\u6362\u6559\u5b66\u4efb\u52a1<\/h2>\n
\u4e0b\u4e00\u8f6e\u7528\u9879\u76ee\u5141\u8bb8\u4f20\u8f93\u7684\u5c0f\u89c4\u6a21\u8f93\u5165\uff0c\u56fa\u5b9a\u8f6f\u4ef6\u7248\u672c\u3001\u53c2\u8003\u3001\u547d\u4ee4\u548c\u9884\u671f\u8f93\u51fa\u3002\u5bf9\u4e8e\u968f\u673a\u6216\u6d6e\u70b9\u8ba1\u7b97\uff0c\u9a8c\u6536\u4e0d\u5e94\u76f2\u76ee\u8981\u6c42\u6240\u6709\u7ed3\u679c\u6587\u4ef6\u9010\u5b57\u8282\u76f8\u540c\uff0c\u5e94\u53e6\u8bbe\u9002\u5408\u8be5\u65b9\u6cd5\u7684\u5173\u952e\u6307\u6807\uff1b\u8f93\u5165\u4f20\u8f93\u6587\u4ef6\u4ecd\u5e94\u505a\u5185\u5bb9\u6821\u9a8c\u3002\u8bb0\u5f55\u6837\u4f8b\u7684\u8d44\u6e90\uff0c\u518d\u7528\u6b63\u5f0f\u89c4\u6a21\u786e\u8ba4\u914d\u7f6e\uff0c\u6559\u5b66\u6837\u4f8b\u7684\u6210\u529f\u4e0d\u4ee3\u8868\u670d\u52a1\u5668\u80fd\u5904\u7406\u5168\u90e8\u6570\u636e\u3002<\/p>\n
\u79df\u671f\u7ed3\u675f\u524d\uff0c\u6309\u6e05\u5355\u53d6\u56de\u590d\u73b0\u6240\u9700\u4ee3\u7801\u3001\u73af\u5883\u7248\u672c\u3001\u53c2\u8003\u8bf4\u660e\u3001\u5173\u952e\u65e5\u5fd7\u548c\u7ed3\u679c\uff0c\u786e\u8ba4\u63a5\u6536\u65b9\u53ef\u4ee5\u6253\u5f00\u548c\u590d\u67e5\uff0c\u518d\u6309\u4e8b\u5148\u7ea6\u5b9a\u7eed\u7528\u6216\u7ed3\u675f\u3002\u4ea4\u63a5\u5b8c\u6210\u5e94\u6709\u660e\u786e\u63a5\u6536\u4eba\u548c\u8bb0\u5f55\uff0c\u4e0d\u80fd\u53ea\u4ee5\u4e0b\u8f7d\u6309\u94ae\u70b9\u8fc7\u4e00\u6b21\u4f5c\u4e3a\u8bc1\u660e\u3002<\/p>\n
\n
\u4e0b\u8f7d\u7ec3\u4e60\u6750\u6599<\/h2>\n
\u4e0b\u8f7d\u6559\u7a0b PDF<\/a> \u00b7 \u4e0b\u8f7d\u4ee3\u7801\u4e0e\u793a\u4f8b\u6570\u636e ZIP<\/a><\/p>\n
\u5148\u6838\u5bf9\u5305\u5185SHA256\u6e05\u5355\uff0c\u518d\u5728\u72ec\u7acb\u76ee\u5f55\u590d\u73b0\u3002<\/p>\n<\/section>\n
\n
\u9700\u8981\u989d\u5916\u7b97\u529b\u65f6<\/h2>\n
\u9002\u7528\u524d\u63d0\uff1a\u5df2\u6709\u4efb\u52a1\u548c\u622a\u6b62\u65e5\u671f\uff0c\u9002\u5408\u9636\u6bb5\u6027\u79df\u8d41\u3002\u5982\u9700\u989d\u5916\u8d44\u6e90\uff0c\u53ef\u8bc4\u4f30\u6309\u9879\u76ee\u5468\u671f\u79df\u8d41\u8ba1\u7b97\u8d44\u6e90\u3002\u54a8\u8be2\u65f6\u8bf4\u660e\u8f6f\u4ef6\u7248\u672c\u3001\u4efb\u52a1\u89c4\u6a21\u548c\u4f7f\u7528\u671f\u9650\uff0c\u914d\u7f6e\u4e0e\u652f\u6301\u8303\u56f4\u53e6\u884c\u786e\u8ba4\u3002<\/p>\n
\n\u54a8\u8be2\u672c\u4efb\u52a1\u7684\u670d\u52a1\u5668\u914d\u7f6e<\/summary>\n
    
\n    
\u54a8\u8be2\u672c\u4efb\u52a1\u7684\u670d\u52a1\u5668\u914d\u7f6e<\/h2>\n    
\u7559\u4e0b\u624b\u673a\u53f7\uff0c\u6211\u4eec\u4f1a\u7ed3\u5408\u672c\u6587\u4efb\u52a1\u4e0e\u60a8\u6c9f\u901a\u670d\u52a1\u5668\u914d\u7f6e\u548c\u62a5\u4ef7\u3002<\/p>\n    
\n    \n    \n    \n