{"id":66539,"date":"2026-11-15T19:45:00","date_gmt":"2026-11-15T11:45:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66539"},"modified":"2026-11-15T14:01:10","modified_gmt":"2026-11-15T06:01:10","slug":"rental-q012-a","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/rental-q012-a\/","title":{"rendered":"\u68c0\u67e5\u5408\u5e76\u524d\u7684\u6570\u636e\u5c42\u3001\u5bf9\u8c61\u5197\u4f59\u548c\u5904\u7406\u987a\u5e8f"},"content":{"rendered":"<p><!-- ysx-rental-campaign:rental-tutorials-400-20261114 article:rental_q012_a --><\/p>\n<div class=\"ysx-rental-tutorial\">\n<figure class=\"ysx-tutorial-figure\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/rental_q012_a-poster.png?resize=1200%2C720&#038;ssl=1\" alt=\"\u5408\u5e76\u4e4b\u524d\u5148\u68c0\u67e5\u6570\u636e\u5c42\uff1a\u6838\u5bf9assay\u4e0elayer\uff1b\u660e\u786e\u5fc5\u987b\u4fdd\u7559\u7684\u5185\u5bb9\uff1b\u5efa\u7acb\u72ec\u7acbcounts\u526f\u672c\uff1b\u68c0\u67e5\u6837\u672c\u4e0e\u7ec6\u80de\u603b\u6570\" width=\"1200\" height=\"720\" loading=\"lazy\"><figcaption>\u672c\u6587\u64cd\u4f5c\u4e0e\u68c0\u67e5\u8def\u5f84\u793a\u610f\u3002<\/figcaption><\/figure>\n<p>\u51e0\u4e2a\u5355\u7ec6\u80de\u5bf9\u8c61\u5404\u81ea\u90fd\u80fd\u6253\u5f00\uff0c\u5408\u5e76\u65f6\u5374\u5185\u5b58\u4e0d\u8db3\uff0c\u5e38\u89c1\u539f\u56e0\u662f\u5408\u5e76\u524d\u540e\u6709\u591a\u4efd\u77e9\u9635\u548c\u4e34\u65f6\u5bf9\u8c61\u540c\u65f6\u5b58\u5728\u3002\u8981\u89e3\u51b3\u95ee\u9898\uff0c\u5148\u786e\u8ba4\u6bcf\u4efd\u5bf9\u8c61\u4fdd\u5b58\u4e86\u4ec0\u4e48\uff0c\u4ee5\u53ca\u5408\u5e76\u540e\u771f\u6b63\u9700\u8981\u54ea\u4e9b\u5185\u5bb9\u3002\u5408\u5e76\u64cd\u4f5c\u672c\u8eab\u5e76\u4e0d\u7b49\u4e8e\u6279\u6b21\u6574\u5408\uff0c\u4e5f\u4e0d\u4f1a\u81ea\u52a8\u66ff\u4f60\u4fdd\u7559\u5168\u90e8\u5206\u6790\u5386\u53f2\u3002<\/p>\n<p>\u672c\u6587\u9762\u5411\u5df2\u6709\u539f\u59cbRNA counts\u3001\u51c6\u5907\u91cd\u65b0\u5f00\u5c55\u8054\u5408\u5206\u6790\u7684\u573a\u666f\u3002\u793a\u4f8b\u4f7f\u7528\u652f\u6301layer\u63a5\u53e3\u7684Seurat\u4e0eSeuratObject\u73af\u5883\uff1b\u8fd0\u884c\u524d\u8bb0\u5f55\u7248\u672c\u548cassay\u7c7b\u3002\u82e5\u5df2\u6709\u591a\u6a21\u6001\u7ed3\u679c\u3001SCT\u6a21\u578b\u6216\u9700\u8981\u4fdd\u7559\u7684\u964d\u7ef4\uff0c\u4e0d\u8981\u76f4\u63a5\u7528counts-only\u91cd\u5efa\u66ff\u6362\u539f\u6587\u4ef6\uff0c\u5e94\u5148\u786e\u5b9a\u540e\u7eed\u5206\u6790\u8def\u5f84\u3002<\/p>\n<h2>\u76d8\u70b9\u5bf9\u8c61\u65f6\uff0c\u4e0d\u8981\u5148\u8f6c\u6210\u666e\u901a\u77e9\u9635<\/h2>\n<p>\u7528\u5bf9\u8c61\u6982\u8981\u3001assay\u4e0elayer\u6e05\u5355\u5b9a\u4f4d\u5360\u7528\u3002<code>object.size<\/code>\u53ea\u80fd\u63d0\u4f9bR\u5bf9\u8c61\u5927\u5c0f\u7684\u8fd1\u4f3c\u53c2\u8003\uff0c\u4e0d\u662f\u8fdb\u7a0b\u5185\u5b58\u5cf0\u503c\uff1b\u78c1\u76d8\u540e\u7aef\u5bf9\u8c61\u8fd8\u53ef\u80fd\u53ea\u8bb0\u5f55\u5916\u90e8\u6570\u636e\u5f15\u7528\u3002\u68c0\u67e5\u77e9\u9635\u7c7b\u5373\u53ef\uff0c\u4e0d\u8981\u4e3a\u4e86\u67e5\u770b\u5b83\u800c\u8c03\u7528<code>as.matrix<\/code>\uff0c\u5426\u5219\u53ef\u80fd\u628a\u7a00\u758f\u6216\u78c1\u76d8\u77e9\u9635\u7269\u5316\u4e3a\u5927\u77e9\u9635\u3002<\/p>\n<pre><code class=\"language-r\">library(Seurat)\nlibrary(SeuratObject)\nobj &lt;- readRDS(&quot;sample01.rds&quot;)\nprint(packageVersion(&quot;Seurat&quot;))\nprint(packageVersion(&quot;SeuratObject&quot;))\nprint(Assays(obj))\nprint(class(obj[[&quot;RNA&quot;]]))\nprint(Layers(obj[[&quot;RNA&quot;]]))\nprint(format(object.size(obj), units = &quot;auto&quot;))\nfor (ly in Layers(obj[[&quot;RNA&quot;]])) {\n  m &lt;- LayerData(obj, assay = &quot;RNA&quot;, layer = ly)\n  print(list(layer = ly, class = class(m), dim = dim(m)))\n}\nwriteLines(capture.output(sessionInfo()), &quot;session-info.txt&quot;)<\/code><\/pre>\n<table>\n<thead>\n<tr>\n<th>\u5185\u5bb9<\/th>\n<th>\u5408\u5e76\u524d\u8981\u95ee\u4ec0\u4e48<\/th>\n<th>\u5904\u7406\u539f\u5219<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>RNA counts<\/td>\n<td>\u662f\u5426\u4e3a\u540c\u4e00\u53c2\u8003\u548c\u540c\u4e00\u8ba1\u6570\u542b\u4e49<\/td>\n<td>\u4f5c\u4e3a\u91cd\u65b0\u5206\u6790\u7684\u660e\u786e\u8f93\u5165<\/td>\n<\/tr>\n<tr>\n<td>data\u4e0escale.data<\/td>\n<td>\u662f\u5426\u91c7\u7528\u517c\u5bb9\u5f52\u4e00\u5316\u3001\u540e\u7eed\u662f\u5426\u4f7f\u7528<\/td>\n<td>\u4e0d\u56e0\u5386\u53f2\u8ba1\u7b97\u8fc7\u5c31\u5168\u90e8\u5408\u5e76<\/td>\n<\/tr>\n<tr>\n<td>SCT\u6216\u5176\u4ed6assay<\/td>\n<td>\u662f\u5426\u5305\u542b\u540e\u7eed\u5fc5\u9700\u6a21\u578b\u6216\u6a21\u6001<\/td>\n<td>\u4e0d\u80fd\u5f53\u65e0\u7528\u5bf9\u8c61\u968f\u610f\u5220\u6389<\/td>\n<\/tr>\n<tr>\n<td>\u964d\u7ef4\u3001\u56fe\u548c\u547d\u4ee4\u8bb0\u5f55<\/td>\n<td>\u662f\u5426\u4f9d\u8d56\u539f\u5355\u6837\u672c\u5206\u6790\u7ed3\u679c<\/td>\n<td>\u6309\u5b98\u65b9\u4fdd\u7559\u8303\u56f4\u53e6\u884c\u5f52\u6863<\/td>\n<\/tr>\n<tr>\n<td>\u6837\u672c\u4e0e\u7ec6\u80de\u6807\u8bc6<\/td>\n<td>\u4e0d\u540c\u6837\u672cbarcode\u662f\u5426\u91cd\u590d<\/td>\n<td>\u5efa\u7acb\u7a33\u5b9a\u524d\u7f00\u548c\u6837\u672c\u5217<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u660e\u786e\u8981\u91cd\u5efa\u65f6\uff0c\u751f\u6210\u72ec\u7acb\u7684counts-only\u526f\u672c<\/h2>\n<p>\u4e0b\u9762\u662f\u4e00\u4e2a\u4fdd\u5b88\u793a\u4f8b\uff1a\u53ea\u63a5\u53d7RNA\u4e2d\u6070\u6709\u4e00\u4e2acounts\u5c42\u7684\u5bf9\u8c61\uff0c\u4fdd\u7559\u7ec6\u80de\u7ea7\u5143\u6570\u636e\u5e76\u589e\u52a0\u6837\u672c\u6807\u8bc6\u3002\u5df2\u6709\u591a\u4e2acounts\u5c42\u65f6\u505c\u6b62\uff0c\u8ba9\u4f7f\u7528\u8005\u5148\u5224\u65ad\u6bcf\u5c42\u5bf9\u5e94\u7684\u6837\u672c\u4e0e\u7ec6\u80de\u96c6\u5408\u3002\u4e0d\u8981\u4e3a\u4e86\u8ba9\u4ee3\u7801\u7ee7\u7eed\u5c31\u65e0\u6761\u4ef6JoinLayers\uff0c\u4e5f\u4e0d\u8981\u628a\u6807\u51c6\u5316\u503c\u5192\u5145\u539f\u59cb\u8ba1\u6570\u3002<\/p>\n<pre><code class=\"language-r\">files &lt;- c(S01 = &quot;sample01.rds&quot;, S02 = &quot;sample02.rds&quot;)\nslim &lt;- lapply(names(files), function(sid) {\n  x &lt;- readRDS(files[[sid]])\n  ly &lt;- grep(&quot;^counts($|\\.)&quot;, Layers(x[[&quot;RNA&quot;]]), value = TRUE)\n  if (length(ly) != 1L) stop(&quot;\u5148\u6838\u5bf9\u5404counts\u5c42\u7684\u6837\u672c\u4e0e\u7ec6\u80de\u5173\u7cfb&quot;)\n  m &lt;- LayerData(x, assay = &quot;RNA&quot;, layer = ly)\n  md &lt;- x[[]][colnames(m), , drop = FALSE]\n  md$sample_id &lt;- sid\n  CreateSeuratObject(counts = m, meta.data = md, project = sid)\n})\nexpected &lt;- sum(vapply(slim, ncol, integer(1)))\nmerged &lt;- merge(slim[[1]], y = slim[-1], add.cell.ids = names(files),\n                merge.data = FALSE, merge.dr = FALSE)\nstopifnot(ncol(merged) == expected, !anyDuplicated(colnames(merged)))\nprint(table(merged$sample_id))\nsaveRDS(merged, &quot;merged-counts-only.rds&quot;)<\/code><\/pre>\n<p>\u6b64\u526f\u672c\u4e0d\u4f1a\u5305\u542b\u539f\u6709\u5f52\u4e00\u5316\u7ed3\u679c\u3001SCT\u6a21\u578b\u548c\u7279\u5f81\u7ea7\u9644\u52a0\u4fe1\u606f\u3002\u539f\u59cb\u6587\u4ef6\u5fc5\u987b\u4fdd\u7559\uff1b\u82e5\u8fd9\u4e9b\u4fe1\u606f\u672c\u6765\u5c31\u662f\u5206\u6790\u8f93\u5165\uff0c\u5e94\u9009\u62e9\u5176\u4ed6\u6709\u660e\u786e\u4fdd\u7559\u89c4\u5219\u7684\u5de5\u4f5c\u6d41\u3002\u5b98\u65b9merge\u8bf4\u660e\u4e5f\u6307\u51fa\uff0c\u56fe\u3001\u8bb0\u5f55\u547d\u4ee4\u548c\u7279\u5f81\u7ea7\u5143\u6570\u636e\u5e76\u4e0d\u4f1a\u5168\u90e8\u968f\u5408\u5e76\u4fdd\u7559\u3002\u57fa\u56e0\u540d\u4e0d\u540c\u6216\u53c2\u8003\u4e0d\u4e00\u81f4\u65f6\uff0c\u66f4\u5e94\u5148\u5904\u7406\u6ce8\u91ca\u95ee\u9898\uff0c\u4e0d\u80fd\u9760\u586b\u96f6\u5408\u5e76\u63a9\u76d6\u5dee\u5f02\u3002<\/p>\n<h2>\u9a8c\u6536\u548c\u5931\u8d25\u6392\u67e5<\/h2>\n<p>\u68c0\u67e5\u7ec6\u80de\u603b\u6570\u662f\u5426\u7b49\u4e8e\u5404\u8f93\u5165\u4e4b\u548c\uff0c\u6837\u672c\u5217\u662f\u5426\u5b8c\u6574\uff0c\u7ec6\u80de\u540d\u662f\u5426\u552f\u4e00\uff0ccounts\u5c42\u7ed3\u6784\u662f\u5426\u7b26\u5408\u4e0b\u4e00\u6b65\u8981\u6c42\u3002\u62bd\u53d6\u5c11\u6570\u5df2\u77e5\u7ec6\u80de\u4e0e\u57fa\u56e0\u6838\u5bf9\u539f\u59cb\u503c\uff0c\u5e76\u5728\u65b0R\u4f1a\u8bdd\u4e2d\u91cd\u65b0\u8bfb\u53d6\u526f\u672c\u3002\u4e0d\u8981\u53ea\u4ee5\u201cR\u6ca1\u6709\u62a5\u9519\u201d\u5224\u65ad\u5408\u5e76\u6b63\u786e\u3002<\/p>\n<h2>\u907f\u514d\u628a\u5408\u5e76\u6210\u529f\u8bef\u5f53\u6210\u6570\u636e\u53ef\u6bd4\u8f83<\/h2>\n<p>\u5408\u5e76\u53ef\u4ee5\u628a\u7279\u5f81\u96c6\u5408\u548c\u7ec6\u80de\u7ec4\u7ec7\u5230\u540c\u4e00\u4e2a\u5bf9\u8c61\uff0c\u4f46\u4e0d\u540c\u53c2\u8003\u7248\u672c\u3001\u4e0d\u540c\u57fa\u56e0\u6807\u8bc6\u6216\u4e0a\u6e38\u8fc7\u6ee4\u89c4\u5219\u4ecd\u53ef\u80fd\u9020\u6210\u4e0d\u4e00\u81f4\u3002\u5c24\u5176\u662f\u4e00\u4e2a\u6837\u672c\u63d0\u524d\u5220\u9664\u4e86\u67d0\u7c7b\u57fa\u56e0\u65f6\uff0c\u7f3a\u5931\u4e0e\u5b9e\u9645\u89c2\u6d4b\u4e3a\u96f6\u4e0d\u662f\u540c\u4e00\u4e2a\u5b9e\u9a8c\u542b\u4e49\u3002\u5e94\u4ece\u5171\u540c\u53c2\u8003\u6216\u6709\u4f9d\u636e\u7684\u6620\u5c04\u89c4\u5219\u5904\u7406\uff0c\u800c\u4e0d\u662f\u4ec5\u51ed\u6700\u7ec8\u77e9\u9635\u51fa\u73b0\u4e86\u76f8\u540c\u540d\u79f0\u5c31\u8ba4\u4e3a\u95ee\u9898\u5df2\u7ecf\u89e3\u51b3\u3002<\/p>\n<p>\u91cd\u65b0\u5f52\u4e00\u5316\u524d\uff0c\u4fdd\u7559\u4e00\u4efd\u53ef\u8ffd\u6eaf\u7684\u6837\u672c\u5230\u7ec6\u80de\u6620\u5c04\u3002\u62bd\u67e5\u65f6\u4ece\u6bcf\u4e2a\u6837\u672c\u6311\u5c11\u91cf\u7ec6\u80de\u548c\u57fa\u56e0\uff0c\u6838\u5bf9\u5408\u5e76\u540e\u5e26\u524d\u7f00\u7684\u7ec6\u80de\u662f\u5426\u5bf9\u5e94\u539f\u503c\uff0c\u540c\u65f6\u786e\u8ba4\u6837\u672c\u3001\u6761\u4ef6\u3001\u4f9b\u4f53\u7b49\u5143\u6570\u636e\u6ca1\u6709\u56e0\u884c\u540d\u987a\u5e8f\u53d8\u5316\u800c\u9519\u4f4d\u3002\u82e5\u5143\u6570\u636e\u91cc\u4fdd\u5b58\u4e86\u65e7\u7684\u805a\u7c7b\u6216\u6ce8\u91ca\uff0c\u5e94\u6ce8\u660e\u5b83\u4eec\u6765\u81ea\u54ea\u6b21\u5355\u6837\u672c\u5206\u6790\uff0c\u4e0d\u80fd\u628a\u65e7\u6807\u7b7e\u81ea\u52a8\u5f53\u4f5c\u8054\u5408\u5206\u6790\u540e\u7684\u6700\u7ec8\u7ed3\u8bba\u3002<\/p>\n<p>\u5bf9\u4e8e\u9700\u8981\u4fdd\u7559\u539f\u964d\u7ef4\u3001\u6a21\u578b\u6216\u591a\u6a21\u6001\u5173\u7cfb\u7684\u4efb\u52a1\uff0ccounts-only\u53ea\u662f\u91cd\u65b0\u5206\u6790\u526f\u672c\u3002\u539f\u5206\u6790\u5bf9\u8c61\u4e0e\u751f\u6210\u811a\u672c\u4ecd\u8981\u5f52\u6863\uff1b\u5220\u9664\u518d\u91cd\u5efa\u4e4b\u540e\uff0c\u4e0d\u80fd\u671f\u5f85\u7f3a\u5931\u7684\u6a21\u578b\u4fe1\u606f\u7531\u5408\u5e76\u51fd\u6570\u6062\u590d\u3002<\/p>\n<p>\u82e5counts-only\u4ecd\u5931\u8d25\uff0c\u8bb0\u5f55\u5931\u8d25\u4f4d\u7f6e\u3001\u5404\u6837\u672c\u89c4\u6a21\u548c\u8fdb\u7a0b\u5185\u5b58\u5cf0\u503c\u3002\u8bfb\u5165\u3001\u5408\u5e76\u3001\u4fdd\u5b58\u4e09\u4e2a\u9636\u6bb5\u53ef\u80fd\u6709\u4e0d\u540c\u9ad8\u5cf0\uff0c\u9010\u9636\u6bb5\u6d4b\u91cf\u624d\u80fd\u77e5\u9053\u662f\u5426\u5b58\u5728\u5bb9\u91cf\u7f3a\u53e3\u3002\u786e\u8ba4\u5fc5\u8981\u6570\u636e\u5df2\u7cbe\u7b80\u3001\u6807\u8bc6\u4e0e\u53c2\u8003\u6b63\u786e\u540e\uff0c\u4ecd\u8d85\u51fa\u672c\u5730\u53ef\u7528\u5185\u5b58\u65f6\uff0c\u53ef\u518d\u6c9f\u901a\u5927\u5185\u5b58\u72ec\u4eabCPU\u670d\u52a1\u5668\uff1b\u4e0d\u8981\u628a\u8f6f\u4ef6\u5c42\u7ed3\u6784\u9519\u8bef\u5f53\u4f5c\u6269\u5bb9\u4f9d\u636e\u3002<\/p>\n<h2>\u53c2\u8003\u4e0e\u7248\u672c\u6838\u5bf9<\/h2>\n<ul>\n<li><a href=\"https:\/\/satijalab.github.io\/seurat-object\/reference\/merge.Seurat.html\">SeuratObject\uff1amerge.Seurat\u53c2\u6570\u4e0e\u4fdd\u7559\u8303\u56f4<\/a><\/li>\n<li><a href=\"https:\/\/satijalab.org\/seurat\/reference\/dietseurat\">Seurat\uff1aDietSeurat\u4e0elayers\u53c2\u6570<\/a><\/li>\n<li><a href=\"https:\/\/satijalab.org\/seurat\/articles\/bpcells_interaction_vignette.html\">Seurat\uff1aBPCells\u78c1\u76d8\u77e9\u9635\u4e0e\u4fdd\u5b58<\/a><\/li>\n<li><a href=\"https:\/\/stat.ethz.ch\/R-manual\/R-devel\/library\/base\/html\/Memory-limits.html\">R\u5b98\u65b9\u624b\u518c\uff1a\u5185\u5b58\u9650\u5236\u4e0e\u5bf9\u8c61<\/a><\/li>\n<\/ul>\n<section class=\"ysx-tutorial-downloads\">\n<h2>\u4e0b\u8f7d\u7ec3\u4e60\u6750\u6599<\/h2>\n<p><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/rental-tutorials-400\/rental_q012_a\/9bbfa60925687993a9f4\/rental_q012_a.pdf\" download>\u4e0b\u8f7d\u6559\u7a0b PDF<\/a> \u00b7 <a href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/rental-tutorials-400\/rental_q012_a\/ebaf7bd77fdb041fd552\/rental_q012_a.zip\" download>\u4e0b\u8f7d\u4ee3\u7801\u4e0e\u793a\u4f8b\u6570\u636e ZIP<\/a><\/p>\n<p>\u5148\u6838\u5bf9\u5305\u5185SHA256\u6e05\u5355\uff0c\u518d\u5728\u72ec\u7acb\u76ee\u5f55\u590d\u73b0\u3002<\/p>\n<\/section>\n<section class=\"ysx-rental-recommendation\">\n<h2>\u9700\u8981\u989d\u5916\u7b97\u529b\u65f6<\/h2>\n<p>\u9002\u7528\u524d\u63d0\uff1a\u51cf\u5c11\u5197\u4f59\u540e\u5408\u5e76\u5cf0\u503c\u4ecd\u8d85\u8fc7\u672c\u5730\u5bb9\u91cf\u3002\u5982\u9700\u989d\u5916\u8d44\u6e90\uff0c\u53ef\u8bc4\u4f30\u5927\u5185\u5b58\u72ec\u4eabCPU\u670d\u52a1\u5668\u79df\u8d41\u3002\u54a8\u8be2\u65f6\u8bf4\u660e\u8f6f\u4ef6\u7248\u672c\u3001\u4efb\u52a1\u89c4\u6a21\u548c\u4f7f\u7528\u671f\u9650\uff0c\u914d\u7f6e\u4e0e\u652f\u6301\u8303\u56f4\u53e6\u884c\u786e\u8ba4\u3002<\/p>\n<details>\n<summary>\u54a8\u8be2\u672c\u4efb\u52a1\u7684\u670d\u52a1\u5668\u914d\u7f6e<\/summary>\n<p>    <section class=\"brtc-section\" id=\"rental-consultation\" aria-labelledby=\"brtc-title-66539\">\n    <h2 id=\"brtc-title-66539\">\u54a8\u8be2\u672c\u4efb\u52a1\u7684\u670d\u52a1\u5668\u914d\u7f6e<\/h2>\n    <p>\u7559\u4e0b\u624b\u673a\u53f7\uff0c\u6211\u4eec\u4f1a\u7ed3\u5408\u672c\u6587\u4efb\u52a1\u4e0e\u60a8\u6c9f\u901a\u670d\u52a1\u5668\u914d\u7f6e\u548c\u62a5\u4ef7\u3002<\/p>\n    <form class=\"brtc-form\" method=\"post\" action=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-admin\/admin-ajax.php\" data-article-key=\"rental_q012_a\">\n    <input type=\"hidden\" name=\"action\" value=\"brtc_submit\"><input type=\"hidden\" name=\"post_id\" value=\"66539\">\n    <input type=\"hidden\" name=\"article_key\" value=\"rental_q012_a\"><input type=\"hidden\" name=\"nonce\" value=\"e658f40451\">\n    <input type=\"hidden\" name=\"request_id\" value=\"a113d63f4daf22f7d40ced7438e4b2f3\"><input type=\"hidden\" name=\"first_key\" value=\"rental_q012_a\">\n    <label>\u624b\u673a\u53f7<input name=\"phone\" type=\"tel\" inputmode=\"numeric\" autocomplete=\"tel-national\" minlength=\"11\" maxlength=\"11\" pattern=\"1[3-9][0-9]{9}\" required placeholder=\"\u8bf7\u8f93\u516511\u4f4d\u624b\u673a\u53f7\" title=\"\u8bf7\u8f93\u5165\u6709\u6548\u768411\u4f4d\u624b\u673a\u53f7\"><\/label>\n    <div class=\"brtc-honeypot\" aria-hidden=\"true\"><label>\u7f51\u7ad9<input name=\"website\" tabindex=\"-1\" autocomplete=\"off\"><\/label><\/div>\n    <label class=\"brtc-consent\"><input type=\"checkbox\" name=\"consent\" value=\"1\" required>\u6211\u540c\u610f\u901a\u8fc7\u6b64\u624b\u673a\u53f7\u8054\u7cfb\u6211\uff0c\u6c9f\u901a\u672c\u6b21\u670d\u52a1\u5668\u914d\u7f6e\u4e0e\u79df\u8d41\u9700\u6c42\u3002<\/label>\n    <button type=\"submit\">\u63d0\u4ea4\u54a8\u8be2<\/button><p class=\"brtc-status\" role=\"status\" aria-live=\"polite\"><\/p>\n    <\/form>\n    <p class=\"brtc-alternative\">\u4e5f\u53ef<a href=\"https:\/\/work.weixin.qq.com\/kfid\/kfcb05492f29d4d6064\" data-ysx-event=\"contact_click\">\u8054\u7cfb\u4e91\u751f\u4fe1\u4f01\u4e1a\u5fae\u4fe1\u5ba2\u670d<\/a>\uff0c\u8bf4\u660e\u672c\u6587\u4efb\u52a1\u3002<\/p>\n    <\/section>\n    <\/details>\n<\/section>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u4eceassay\u4e0elayer\u76d8\u70b9\u5f00\u59cb\uff0c\u5728\u660e\u786e\u91cd\u65b0\u5206\u6790\u7684\u524d\u63d0\u4e0b\u5efa\u7acbcounts-only\u526f\u672c\uff0c\u9a8c\u8bc1\u6837\u672c\u3001\u7ec6\u80de\u6570\u548c\u4fdd\u7559\u8303\u56f4\u540e\u518d\u51b3\u5b9a\u662f\u5426\u6269\u5bb9\u3002<\/p>\n","protected":false},"author":1,"featured_media":66538,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[1,71,75],"tags":[551,156],"class_list":["post-66539","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-part","category-single-cell","category-compute-environment","tag-seuratr","tag-156"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/rental_q012_a-poster.png?fit=1200%2C720&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66539","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=66539"}],"version-history":[{"count":6,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66539\/revisions"}],"predecessor-version":[{"id":73110,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/66539\/revisions\/73110"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/66538"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=66539"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=66539"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=66539"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}