{"id":66545,"date":"2026-11-15T20:30:00","date_gmt":"2026-11-15T12:30:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66545"},"modified":"2026-11-15T14:01:10","modified_gmt":"2026-11-15T06:01:10","slug":"rental-q013-b","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/rental-q013-b\/","title":{"rendered":"\u5728\u4ee3\u8868\u6027\u5b50\u96c6\u4e0a\u6bd4\u8f83\u5185\u5b58\u7b56\u7565\u4e0e\u670d\u52a1\u5668\u89c4\u683c"},"content":{"rendered":"<p><!-- ysx-rental-campaign:rental-tutorials-400-20261114 article:rental_q013_b --><\/p>\n<div class=\"ysx-rental-tutorial\">\n<figure class=\"ysx-tutorial-figure\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/rental_q013_b-poster.png?resize=1200%2C720&#038;ssl=1\" alt=\"\u7528\u540c\u4e00\u5b50\u96c6\u6bd4\u8f83SCT\u7b56\u7565\uff1a\u4fdd\u5b58\u8bca\u65ad\u7ec6\u80de\u6e05\u5355\uff1b\u72ec\u7acb\u8fdb\u7a0b\u6bd4\u8f83\u7b56\u7565\uff1b\u68c0\u67e5\u8f93\u51fa\u548c\u8fd0\u884c\u65e5\u5fd7\uff1b\u6269\u5927\u89c4\u6a21\u590d\u9a8c\u5019\u9009\u8d44\u6e90\" width=\"1200\" height=\"720\" loading=\"lazy\"><figcaption>\u672c\u6587\u64cd\u4f5c\u4e0e\u68c0\u67e5\u8def\u5f84\u793a\u610f\u3002<\/figcaption><\/figure>\n<p>SCTransform\u9700\u8981\u591a\u5c11\u5185\u5b58\uff0c\u5e94\u8be5\u901a\u8fc7\u4e0e\u4f60\u7684\u6570\u636e\u7ed3\u6784\u548c\u53c2\u6570\u5339\u914d\u7684\u8bb0\u5f55\u6765\u5224\u65ad\u3002\u53e6\u4e00\u7bc7\u6587\u7ae0\u53ef\u4ee5\u89e3\u51b3\u62a5\u9519\u5b9a\u4f4d\uff1b\u672c\u6587\u8fdb\u4e00\u6b65\u7ec4\u7ec7\u4e00\u4e2a\u53ef\u6bd4\u8f83\u7684\u8bd5\u9a8c\uff1a\u5728\u76f8\u540c\u7ec6\u80de\u5b50\u96c6\u4e0a\uff0c\u6bd4\u8f83\u5185\u5b58\u7b56\u7565\u3001\u8fd0\u884c\u8017\u65f6\u548c\u8f93\u51fa\u8981\u6c42\uff0c\u518d\u7528\u8f83\u5927\u89c4\u6a21\u590d\u9a8c\u5019\u9009\u670d\u52a1\u5668\u3002<\/p>\n<p>\u5b50\u96c6\u8bd5\u8dd1\u7528\u6765\u6d4b\u7b97\u4e0e\u8bca\u65ad\uff0c\u4e0d\u76f4\u63a5\u66ff\u4ee3\u6b63\u5f0f\u5206\u6790\u3002\u62bd\u6837\u53ef\u80fd\u9057\u6f0f\u7a00\u6709\u7ec6\u80de\u3001\u6539\u53d8\u53d8\u5f02\u7279\u5f81\u548c\u6a21\u578b\u4f30\u8ba1\uff1b\u5982\u679c\u6539\u53d8\u4e86\u7814\u7a76\u76ee\u6807\u6216\u7edf\u8ba1\u6761\u4ef6\uff0c\u5e94\u53e6\u4f5c\u5206\u6790\u5224\u65ad\uff0c\u4e0d\u80fd\u5c06\u66f4\u5c0f\u7684\u8ba1\u7b97\u7ed3\u679c\u5192\u5145\u5168\u91cf\u4efb\u52a1\u3002<\/p>\n<h2>\u51c6\u5907\u56fa\u5b9a\u4e14\u53ef\u8ffd\u6eaf\u7684\u6d4b\u8bd5\u8f93\u5165<\/h2>\n<p>\u4ece\u5df2\u7ecf\u68c0\u67e5\u8fc7\u7684RNA\u539f\u59cbcounts\u5bf9\u8c61\u751f\u6210\u5b50\u96c6\u3002\u4f18\u5148\u4fdd\u7559\u4e0d\u540c\u6837\u672c\u3001\u6d4b\u5e8f\u6df1\u5ea6\u548c\u91cd\u8981\u7ec6\u80de\u7fa4\u7684\u4ee3\u8868\u6027\u3002\u4e0b\u9762\u6309\u6837\u672c\u5206\u5c42\u9009\u53d6\u4e00\u4e2a\u8bca\u65ad\u5b50\u96c6\uff0c\u5e76\u4fdd\u5b58\u7ec6\u80deID\uff1b\u6bcf\u4e2a\u6837\u672c\u6700\u591a\u53d6\u4e00\u5b9a\u6570\u91cf\u4ec5\u662f\u8bd5\u9a8c\u793a\u4f8b\uff0c\u4e0d\u662f\u6700\u4f73\u62bd\u6837\u89c4\u6a21\u3002<\/p>\n<pre><code class=\"language-r\">library(Seurat)\nset.seed(2026)\nobj &lt;- readRDS(&quot;rna-counts.rds&quot;)\nstopifnot(&quot;sample_id&quot; %in% colnames(obj[[]]))\ngroups &lt;- split(colnames(obj), obj$sample_id)\nselected &lt;- unlist(lapply(groups, function(cells) {\n  sample(cells, min(length(cells), 1000L))\n}), use.names = FALSE)\nwriteLines(selected, &quot;pilot-cells.txt&quot;)\npilot &lt;- subset(obj, cells = selected)\nsaveRDS(pilot, &quot;sct-pilot.rds&quot;)<\/code><\/pre>\n<p>\u82e5\u6837\u672c\u4e0e\u7ec6\u80de\u7fa4\u6807\u8bc6\u5df2\u6709\u8d28\u91cf\u95ee\u9898\uff0c\u5148\u4fee\u590d\u6e05\u5355\u3002\u76f8\u540c\u6d4b\u8bd5\u8f93\u5165\u5e94\u88ab\u4e24\u79cd\u7b56\u7565\u5171\u540c\u8bfb\u53d6\uff0c\u4e0d\u8981\u5206\u522b\u968f\u673a\u62bd\u6837\u540e\u6bd4\u8f83\u3002\u8f93\u5165\u8fd8\u9700\u660e\u786ecounts\u5c42\u7ed3\u6784\uff1b\u591a\u4e2a\u6837\u672c\u5728\u4e00\u4e2acounts\u5c42\u4e0e\u6bcf\u6837\u672c\u72ec\u7acbcounts\u5c42\u4e0d\u4e00\u5b9a\u8d70\u76f8\u540c\u5efa\u6a21\u8def\u5f84\uff0c\u5e94\u4f5c\u4e3a\u4e0d\u540c\u5de5\u4f5c\u6d41\u5206\u522b\u8bb0\u5f55\u3002<\/p>\n<h2>\u6bcf\u4e2a\u7b56\u7565\u5728\u65b0\u7684R\u8fdb\u7a0b\u4e2d\u8fd0\u884c<\/h2>\n<p>\u5c06\u4e0b\u9762\u811a\u672c\u4fdd\u5b58\u4e3a<code>pilot_sct.R<\/code>\u3002\u5b83\u628a\u8bca\u65ad\u8303\u56f4\u9650\u5236\u5728\u5355counts\u5c42\uff0c\u4fdd\u6301\u8f93\u51fa\u8bbe\u7f6e\u548c\u7248\u672c\u4e00\u81f4\uff0c\u53ea\u6539\u53d8\u662f\u5426\u91c7\u7528conserve.memory\u3002\u4e0d\u8981\u540c\u65f6\u8fd0\u884c\u4e24\u4e2a\u6d4b\u8bd5\uff0c\u5426\u5219\u8d44\u6e90\u4e89\u7528\u4f1a\u5e72\u6270\u6bd4\u8f83\u3002<\/p>\n<pre><code class=\"language-r\">library(Seurat)\nargs &lt;- commandArgs(trailingOnly = TRUE)\nstopifnot(length(args) == 3L, args[2] %in% c(&quot;TRUE&quot;, &quot;FALSE&quot;))\nx &lt;- readRDS(args[1])\nly &lt;- grep(&quot;^counts($|\\.)&quot;, Layers(x[[&quot;RNA&quot;]]), value = TRUE)\nif (length(ly) != 1L) stop(&quot;\u5148\u786e\u8ba4\u5355\u5c42\u8bca\u65ad\u8f93\u5165&quot;)\nif (requireNamespace(&quot;future&quot;, quietly = TRUE)) future::plan(future::sequential)\ny &lt;- SCTransform(x, assay = &quot;RNA&quot;, vst.flavor = &quot;v2&quot;,\n                 variable.features.n = 3000,\n                 return.only.var.genes = TRUE,\n                 conserve.memory = as.logical(args[2]), verbose = TRUE)\nsaveRDS(y, args[3])\nwriteLines(capture.output(sessionInfo()), paste0(args[3], &quot;.session.txt&quot;))<\/code><\/pre>\n<pre><code class=\"language-bash\">\/usr\/bin\/time -v -o sct-default.time.txt   Rscript pilot_sct.R sct-pilot.rds FALSE pilot-default.rds   &gt;sct-default.out.txt 2&gt;sct-default.err.txt\n\/usr\/bin\/time -v -o sct-conserve.time.txt   Rscript pilot_sct.R sct-pilot.rds TRUE pilot-conserve.rds   &gt;sct-conserve.out.txt 2&gt;sct-conserve.err.txt<\/code><\/pre>\n<table>\n<thead>\n<tr>\n<th>\u6bd4\u8f83\u7ef4\u5ea6<\/th>\n<th>\u5e94\u8bb0\u5f55\u7684\u5185\u5bb9<\/th>\n<th>\u9a8c\u6536\u539f\u5219<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\u8f93\u5165<\/td>\n<td>\u7ec6\u80de\u6e05\u5355\u3001\u6837\u672c\u3001counts\u5c42\u4e0e\u53c2\u8003<\/td>\n<td>\u4e24\u79cd\u7b56\u7565\u8bfb\u53d6\u540c\u4e00\u4efd\u8f93\u5165<\/td>\n<\/tr>\n<tr>\n<td>\u8fd0\u884c<\/td>\n<td>\u8f6f\u4ef6\u7248\u672c\u3001\u4e32\u884c\u6216\u5e76\u884c\u3001\u8017\u65f6\u4e0e\u5cf0\u503c<\/td>\n<td>\u53ea\u6539\u53d8\u5df2\u8ba1\u5212\u7684\u56e0\u7d20<\/td>\n<\/tr>\n<tr>\n<td>\u8f93\u51fa<\/td>\n<td>\u7ec6\u80de\u6570\u3001\u5fc5\u8981\u7279\u5f81\u3001\u6a21\u578b\u4e0e\u4e0b\u4e00\u6b65<\/td>\n<td>\u80fd\u652f\u6301\u65e2\u5b9a\u5206\u6790\u8def\u5f84<\/td>\n<\/tr>\n<tr>\n<td>\u6269\u5927\u89c4\u6a21<\/td>\n<td>\u66f4\u591a\u7ec6\u80de\u6216\u5b8c\u6574\u6837\u672c\u7684\u590d\u9a8c<\/td>\n<td>\u4e0d\u6309\u5c0f\u5b50\u96c6\u7ebf\u6027\u5916\u63a8\u4fdd\u8bc1<\/td>\n<\/tr>\n<tr>\n<td>\u90e8\u7f72<\/td>\n<td>\u53ef\u7528\u5185\u5b58\u3001\u4e34\u65f6\u76d8\u3001\u4efb\u52a1\u5e76\u53d1<\/td>\n<td>\u8d44\u6e90\u8303\u56f4\u4e0e\u6b63\u5f0f\u4efb\u52a1\u4e00\u81f4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u600e\u6837\u8bfb\u7ed3\u679c\u800c\u4e0d\u8bef\u5224<\/h2>\n<p>\u5148\u5254\u9664\u5931\u8d25\u8fd0\u884c\uff0c\u518d\u6bd4\u8f83\u6709\u6548\u8bb0\u5f55\u3002\u82e5\u5185\u5b58\u7b56\u7565\u964d\u4f4e\u4e86\u5cf0\u503c\u4f46\u589e\u52a0\u8017\u65f6\uff0c\u6309\u7167\u5b9e\u9645\u622a\u6b62\u65f6\u95f4\u548c\u8d39\u7528\u6761\u4ef6\u53d6\u820d\u3002\u82e5\u4e24\u7ec4\u8f93\u51fa\u7279\u5f81\u6216\u6a21\u578b\u7ed3\u6784\u4e0d\u540c\uff0c\u5148\u67e5\u660e\u7248\u672c\u548c\u53c2\u6570\u8def\u5f84\uff0c\u4e0d\u628a\u5b83\u4eec\u76f4\u63a5\u4f5c\u4e3a\u7b49\u4ef7\u4efb\u52a1\u6bd4\u4ef7\u3002\u4fdd\u7559\u539f\u59cb\u65e5\u5fd7\uff0c\u4e0d\u4ec5\u8bb0\u4e00\u4e2a\u201c\u6210\u529f\u201d\u3002<\/p>\n<p>\u968f\u540e\u589e\u52a0\u6d4b\u8bd5\u89c4\u6a21\uff0c\u89c2\u5bdf\u5cf0\u503c\u53d1\u751f\u5728\u54ea\u4e2a\u9636\u6bb5\u3002\u67d0\u4e9b\u77e9\u9635\u64cd\u4f5c\u3001\u6a21\u578b\u6574\u7406\u6216\u591a\u5c42\u6c47\u603b\u53ef\u80fd\u5bfc\u81f4\u975e\u7ebf\u6027\u589e\u957f\uff0c\u6570\u636e\u91cf\u589e\u52a0\u4e00\u500d\u4e0d\u4fdd\u8bc1\u5185\u5b58\u53ea\u589e\u4e00\u500d\u3002\u5019\u9009\u89c4\u683c\u5e94\u81f3\u5c11\u63a5\u53d7\u63a5\u8fd1\u6b63\u5f0f\u89c4\u6a21\u7684\u590d\u9a8c\uff0c\u5e76\u5728\u8ba1\u5212\u5e76\u53d1\u65f6\u68c0\u67e5\u603b\u8d44\u6e90\u3002<\/p>\n<h2>\u8ba9\u4ee3\u8868\u6027\u8986\u76d6\u6570\u636e\u7ed3\u6784\uff0c\u800c\u4e0d\u53ea\u662f\u7ec6\u80de\u6570\u91cf<\/h2>\n<p>\u540c\u6837\u6570\u91cf\u7684\u7ec6\u80de\uff0c\u6d4b\u5e8f\u6df1\u5ea6\u3001\u975e\u96f6\u57fa\u56e0\u5206\u5e03\u548c\u6837\u672c\u5206\u5c42\u4e5f\u53ef\u80fd\u4e0d\u540c\u3002\u5efa\u8bae\u5728\u8f83\u5c0f\u8bca\u65ad\u96c6\u5408\u4e4b\u5916\uff0c\u52a0\u5165\u4e00\u4efd\u63a5\u8fd1\u4e0a\u754c\u7684\u6b63\u5f0f\u6837\u672c\uff0c\u5e76\u8bb0\u5f55\u6bcf\u4e2a\u8f93\u5165\u7684\u5c42\u6570\u91cf\u3001\u7ec6\u80de\u91cf\u4e0e\u7279\u5f81\u8303\u56f4\u3002\u82e5\u51c6\u5907\u6bd4\u8f83\u5355\u5c42\u548c\u591a\u5c42\u5de5\u4f5c\u6d41\uff0c\u5206\u522b\u4fdd\u7559\u5b9e\u9a8c\u7ec4\uff0c\u4e0d\u80fd\u628a\u5b83\u4eec\u6df7\u8fdb\u540c\u4e00\u6761\u201c\u6bcf\u4e2a\u7ec6\u80de\u9700\u8981\u591a\u5c11\u5185\u5b58\u201d\u7684\u66f2\u7ebf\u3002<\/p>\n<p>\u9a8c\u6536\u4e0d\u80fd\u53ea\u68c0\u67e5\u8f93\u51fa\u6587\u4ef6\u5927\u5c0f\u3002\u5bf9\u76f8\u540c\u8f93\u5165\uff0c\u6838\u5bf9\u7ec6\u80de\u3001\u6240\u9700\u57fa\u56e0\u4e0e\u6a21\u578b\u4fe1\u606f\uff0c\u5e76\u5728\u540e\u7eedPCA\u6216\u8ba1\u5212\u6b65\u9aa4\u4e0a\u8dd1\u4e00\u6b21\u5c0f\u9a8c\u8bc1\u3002\u53d1\u73b0\u53d8\u5f02\u7279\u5f81\u6216\u6570\u503c\u5dee\u5f02\u65f6\uff0c\u5148\u68c0\u67e5\u6a21\u578b\u548c\u7248\u672c\u8def\u5f84\uff0c\u5224\u65ad\u662f\u5426\u5f71\u54cd\u76ee\u6807\uff0c\u800c\u4e0d\u662f\u5f3a\u5236\u4e24\u4efd\u7ed3\u679c\u9010\u5b57\u8282\u76f8\u540c\u3002\u540c\u65f6\u8bb0\u5f55\u53c2\u8003\u6765\u6e90\u4e0e\u6570\u636e\u6587\u4ef6\u6821\u9a8c\u503c\uff0c\u6392\u9664\u8f93\u5165\u5df2\u7ecf\u6539\u53d8\u7684\u60c5\u51b5\u3002<\/p>\n<p>\u5982\u679c\u4e00\u7ec4\u8bd5\u8dd1\u5931\u8d25\uff0c\u4fdd\u7559\u539f\u53c2\u6570\u548c\u5931\u8d25\u65e5\u5fd7\uff0c\u518d\u65b0\u589e\u4fee\u8ba2\u7ec4\u3002\u4e0d\u8981\u5728\u65e5\u5fd7\u4e4b\u5916\u6084\u6084\u51cf\u5c11\u7ec6\u80de\u3001\u5220\u6389\u56de\u5f52\u9879\u540e\uff0c\u4ecd\u628a\u5b83\u8bb0\u4f5c\u539f\u4efb\u52a1\u6210\u529f\uff1b\u8fd9\u6837\u7684\u8bb0\u5f55\u65e0\u6cd5\u652f\u6301\u6b63\u5f0f\u8d44\u6e90\u9009\u62e9\u3002<\/p>\n<p>\u6700\u540e\u8f93\u51fa\u4e00\u5f20\u8d44\u6e90\u62a5\u544a\uff0c\u5217\u51fa\u8f93\u5165\u89c4\u6a21\u3001counts\u5c42\u7ed3\u6784\u3001\u53c2\u6570\u3001\u6709\u6548\u8017\u65f6\u3001\u5185\u5b58\u9ad8\u6c34\u4f4d\u3001\u8f93\u51fa\u9a8c\u6536\u4e0e\u672a\u9a8c\u8bc1\u8303\u56f4\u3002\u5fc5\u8981\u4efb\u52a1\u4ecd\u6709\u5bb9\u91cf\u7f3a\u53e3\u65f6\uff0c\u53ef\u4ee5\u636e\u6b64\u6c9f\u901a\u5927\u5185\u5b58\u72ec\u4eabCPU\u79df\u8d41\u3002\u670d\u52a1\u5668\u5185\u5b58\u4e0e\u8fd0\u884c\u73af\u5883\u90fd\u5e94\u5199\u5165\u786e\u8ba4\u8303\u56f4\uff0c\u4e0d\u7528\u4e00\u6b21\u5c0f\u6837\u672c\u6210\u529f\u66ff\u4ee3\u6b63\u5f0f\u4ea4\u4ed8\u9a8c\u6536\u3002<\/p>\n<h2>\u53c2\u8003\u4e0e\u7248\u672c\u6838\u5bf9<\/h2>\n<ul>\n<li><a href=\"https:\/\/satijalab.org\/seurat\/reference\/sctransform\">Seurat\uff1aSCTransform\u53c2\u8003<\/a><\/li>\n<li><a href=\"https:\/\/satijalab.org\/seurat\/articles\/sctransform_vignette.html\">Seurat\uff1aSCTransform\u5de5\u4f5c\u6d41<\/a><\/li>\n<li><a href=\"https:\/\/www.gnu.org\/software\/time\/manual\/html_node\/Memory-Resources.html\">GNU Time\uff1a\u5185\u5b58\u8d44\u6e90\u7edf\u8ba1<\/a><\/li>\n<li><a href=\"https:\/\/stat.ethz.ch\/R-manual\/R-devel\/library\/base\/html\/Memory-limits.html\">R\u5b98\u65b9\u624b\u518c\uff1a\u5185\u5b58\u9650\u5236\u4e0e\u5bf9\u8c61<\/a><\/li>\n<\/ul>\n<section class=\"ysx-tutorial-downloads\">\n<h2>\u4e0b\u8f7d\u7ec3\u4e60\u6750\u6599<\/h2>\n<p><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/rental-tutorials-400\/rental_q013_b\/373c7fc2ce552ac3831a\/rental_q013_b.pdf\" download>\u4e0b\u8f7d\u6559\u7a0b PDF<\/a> \u00b7 <a href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/rental-tutorials-400\/rental_q013_b\/2fd94b2d8e986a7c4306\/rental_q013_b.zip\" download>\u4e0b\u8f7d\u4ee3\u7801\u4e0e\u793a\u4f8b\u6570\u636e ZIP<\/a><\/p>\n<p>\u5148\u6838\u5bf9\u5305\u5185SHA256\u6e05\u5355\uff0c\u518d\u5728\u72ec\u7acb\u76ee\u5f55\u590d\u73b0\u3002<\/p>\n<\/section>\n<section class=\"ysx-rental-recommendation\">\n<h2>\u9700\u8981\u989d\u5916\u7b97\u529b\u65f6<\/h2>\n<p>\u9002\u7528\u524d\u63d0\uff1a\u7248\u672c\u548c\u53c2\u6570\u6838\u5bf9\u540e\u4ecd\u5b58\u5728\u5bb9\u91cf\u7f3a\u53e3\u3002\u5982\u9700\u989d\u5916\u8d44\u6e90\uff0c\u53ef\u8bc4\u4f30\u5927\u5185\u5b58\u72ec\u4eabCPU\u670d\u52a1\u5668\u79df\u8d41\u3002\u54a8\u8be2\u65f6\u8bf4\u660e\u8f6f\u4ef6\u7248\u672c\u3001\u4efb\u52a1\u89c4\u6a21\u548c\u4f7f\u7528\u671f\u9650\uff0c\u914d\u7f6e\u4e0e\u652f\u6301\u8303\u56f4\u53e6\u884c\u786e\u8ba4\u3002<\/p>\n<details>\n<summary>\u54a8\u8be2\u672c\u4efb\u52a1\u7684\u670d\u52a1\u5668\u914d\u7f6e<\/summary>\n<p>    <section class=\"brtc-section\" id=\"rental-consultation\" aria-labelledby=\"brtc-title-66545\">\n    <h2 id=\"brtc-title-66545\">\u54a8\u8be2\u672c\u4efb\u52a1\u7684\u670d\u52a1\u5668\u914d\u7f6e<\/h2>\n    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