{"id":66555,"date":"2026-11-15T21:45:00","date_gmt":"2026-11-15T13:45:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66555"},"modified":"2026-11-15T14:01:11","modified_gmt":"2026-11-15T06:01:11","slug":"rental-q016-a","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/rental-q016-a\/","title":{"rendered":"\u68c0\u67e5\u7f29\u653e\u7279\u5f81\u8303\u56f4\u3001\u77e9\u9635\u8868\u793a\u4e0e\u4e34\u65f6\u5bf9\u8c61"},"content":{"rendered":"<p><!-- ysx-rental-campaign:rental-tutorials-400-20261114 article:rental_q016_a --><\/p>\n<div class=\"ysx-rental-tutorial\">\n<figure class=\"ysx-tutorial-figure\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/rental_q016_a-poster.png?resize=1200%2C720&#038;ssl=1\" alt=\"ScaleData\u5148\u6838\u5bf9\u7279\u5f81\u8303\u56f4\uff1a\u68c0\u67e5features\u4e0edata\u5c42\uff1b\u8fa8\u8ba4\u77e9\u9635\u7c7b\u548c\u540e\u7aef\uff1b\u5355\u56e0\u7d20\u6d4b\u8bd5\u5206\u5757\uff1b\u9a8c\u6536\u7ed3\u679c\u540e\u518d\u6269\u5bb9\" width=\"1200\" height=\"720\" loading=\"lazy\"><figcaption>\u672c\u6587\u64cd\u4f5c\u4e0e\u68c0\u67e5\u8def\u5f84\u793a\u610f\u3002<\/figcaption><\/figure>\n<p>\u539f\u59cbcounts\u662f\u7a00\u758f\u77e9\u9635\uff0c\u5e76\u4e0d\u610f\u5473\u7740ScaleData\u7684\u6240\u6709\u7ed3\u679c\u90fd\u540c\u6837\u7a00\u758f\u3002\u4e2d\u5fc3\u5316\u3001\u7f29\u653e\u6216\u56de\u5f52\u540e\u7684\u6570\u636e\u53ef\u80fd\u6709\u4e0d\u540c\u8868\u793a\uff0c\u6240\u9009\u7279\u5f81\u6570\u548c\u7ec6\u80de\u6570\u4e5f\u4f1a\u76f4\u63a5\u5f71\u54cd\u9700\u8981\u5b58\u653e\u7684\u7ed3\u679c\u3002\u9047\u5230\u5185\u5b58\u4e0a\u5347\u65f6\uff0c\u5148\u68c0\u67e5\u5b9e\u9645\u8ba1\u7b97\u8303\u56f4\uff0c\u518d\u5224\u65ad\u662f\u5426\u771f\u7684\u9700\u8981\u66f4\u591a\u5bb9\u91cf\u3002<\/p>\n<p>\u672c\u6587\u8ba8\u8bba\u5e38\u89c4RNA\u7684NormalizeData\u3001\u7279\u5f81\u9009\u62e9\u548cScaleData\u8def\u5f84\u3002SCTransform\u6709\u81ea\u5df1\u7684\u6a21\u578b\u4e0e\u6b8b\u5dee\u5904\u7406\uff0c\u4e0d\u5e94\u673a\u68b0\u5730\u5728SCT\u4e4b\u540e\u91cd\u590d\u540c\u4e00\u4e32\u6b65\u9aa4\u3002\u5f53\u524dSeurat\u8fd8\u5b58\u5728\u9762\u5411IterableMatrix\u7684\u4e13\u95e8\u5b9e\u73b0\uff0c\u5185\u5b58\u578b\u77e9\u9635\u7684\u7ecf\u9a8c\u4e0d\u80fd\u4e0d\u52a0\u68c0\u67e5\u5730\u5957\u5230\u78c1\u76d8\u540e\u7aef\u3002<\/p>\n<h2>\u5148\u5217\u51fa\u5b9e\u9645\u8981\u7f29\u653e\u7684\u7279\u5f81<\/h2>\n<p>\u67e5\u770b\u9ed8\u8ba4assay\u3001VariableFeatures\u3001data\u5c42\u548c\u77e9\u9635\u7c7b\u3002\u67d0\u4efd\u811a\u672c\u663e\u5f0f\u4f20\u5165\u5168\u90e8\u57fa\u56e0\u65f6\uff0c\u5b83\u4e0e\u4ec5\u7f29\u653e\u9009\u5b9a\u53d8\u5f02\u7279\u5f81\u7684\u4efb\u52a1\u89c4\u6a21\u4e0d\u540c\u3002\u5982\u679c\u540e\u7eedPCA\u53ea\u4f7f\u7528\u5408\u7406\u7684\u7279\u5f81\u96c6\u5408\uff0c\u9996\u5148\u6838\u5bf9\u662f\u5426\u6709\u5fc5\u8981\u521b\u5efa\u5168\u7279\u5f81\u7ed3\u679c\uff1b\u82e5\u7814\u7a76\u65b9\u6cd5\u786e\u5b9e\u8981\u6c42\u66f4\u591a\u7279\u5f81\uff0c\u5e94\u4fdd\u7559\u7406\u7531\uff0c\u800c\u4e0d\u662f\u53ea\u4e3a\u4e86\u7701\u5185\u5b58\u968f\u610f\u5220\u6389\u76ee\u6807\u3002<\/p>\n<pre><code class=\"language-r\">library(Seurat)\nobj &lt;- readRDS(&quot;rna-normalized.rds&quot;)\nDefaultAssay(obj) &lt;- &quot;RNA&quot;\nprint(packageVersion(&quot;Seurat&quot;))\nprint(class(obj[[&quot;RNA&quot;]]))\nprint(Layers(obj[[&quot;RNA&quot;]]))\nly &lt;- grep(&quot;^data($|\\.)&quot;, Layers(obj[[&quot;RNA&quot;]]), value=TRUE)\nif (length(ly) != 1L) stop(&quot;\u6b64\u5355\u5c42\u793a\u4f8b\u9700\u5148\u6838\u5bf9\u591a\u4e2adata\u5c42\u7684\u5904\u7406\u8def\u5f84&quot;)\nm &lt;- LayerData(obj, assay=&quot;RNA&quot;, layer=ly)\nprint(list(class=class(m), dim=dim(m)))\nfeatures &lt;- VariableFeatures(obj)\nstopifnot(length(features)&gt;0L, all(features %in% rownames(m)))\nprint(length(features))\nrm(m)<\/code><\/pre>\n<p>\u7f3a\u5c11\u53d8\u5f02\u7279\u5f81\u65f6\uff0c\u5148\u6309\u7167\u8ba1\u5212\u7684\u65b9\u6cd5\u5b8c\u6210\u9009\u62e9\u3002\u4e0d\u8981\u7b80\u5355\u628a\u7a7a\u96c6\u5408\u66ff\u6362\u6210\u6240\u6709\u57fa\u56e0\uff0c\u8ba9\u4ee3\u7801\u7ee7\u7eed\u6267\u884c\uff1b\u8fd9\u53ef\u80fd\u65e2\u6539\u53d8\u65b9\u6cd5\u4e5f\u653e\u5927\u5185\u5b58\u3002\u82e5\u5b58\u5728\u591a\u4e2adata\u5c42\u6216\u78c1\u76d8\u77e9\u9635\uff0c\u5e94\u56de\u5230\u5bf9\u5e94\u7248\u672c\u4e0eassay\u5b9e\u73b0\u68c0\u67e5\uff0c\u800c\u4e0d\u662f\u4e3a\u4e86\u83b7\u53d6\u77e9\u9635\u76f4\u63a5\u5f3a\u5236JoinLayers\u6216as.matrix\u3002<\/p>\n<table>\n<thead>\n<tr>\n<th>\u68c0\u67e5\u70b9<\/th>\n<th>\u5b83\u4e3a\u4f55\u5f71\u54cd\u5360\u7528<\/th>\n<th>\u5e94\u91c7\u53d6\u7684\u5224\u65ad<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>features<\/td>\n<td>\u51b3\u5b9a\u7ed3\u679c\u5305\u542b\u591a\u5c11\u7279\u5f81<\/td>\n<td>\u4e0e\u4e0b\u4e00\u6b65\u5206\u6790\u8981\u6c42\u5bf9\u9f50<\/td>\n<\/tr>\n<tr>\n<td>\u77e9\u9635\u7c7b\u548c\u540e\u7aef<\/td>\n<td>\u51b3\u5b9a\u5b58\u50a8\u4e0e\u8ba1\u7b97\u5b9e\u73b0<\/td>\n<td>\u4e0d\u8981\u4e3b\u52a8\u7a20\u5bc6\u5316<\/td>\n<\/tr>\n<tr>\n<td>vars.to.regress<\/td>\n<td>\u589e\u52a0\u6a21\u578b\u8ba1\u7b97\u4e0e\u4e34\u65f6\u6570\u636e<\/td>\n<td>\u53ea\u6309\u7814\u7a76\u8bbe\u8ba1\u9009\u62e9\u534f\u53d8\u91cf<\/td>\n<\/tr>\n<tr>\n<td>block.size<\/td>\n<td>\u90e8\u5206\u5b9e\u73b0\u4e2d\u5f71\u54cd\u5355\u6b21\u7279\u5f81\u5757<\/td>\n<td>\u53ef\u6bd4\u8f83\u5cf0\u503c\uff0c\u4f46\u4e0d\u80fd\u6d88\u9664\u6700\u7ec8\u8f93\u51fa<\/td>\n<\/tr>\n<tr>\n<td>\u5df2\u6709scale.data<\/td>\n<td>\u65e7\u7ed3\u679c\u4e0e\u65b0\u7ed3\u679c\u53ef\u80fd\u540c\u65f6\u5b58\u5728<\/td>\n<td>\u5728\u72ec\u7acb\u526f\u672c\u4e2d\u6d4b\u8bd5\u5e76\u8bb0\u5f55\u5cf0\u503c<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u5728\u660e\u786e\u7684\u5185\u5b58\u77e9\u9635\u8def\u5f84\u4e0a\u6d4b\u8bd5\u8303\u56f4<\/h2>\n<p>\u4e0b\u9762\u793a\u4f8b\u4ec5\u9002\u7528\u4e8e\u5df2\u7ecf\u9a8c\u6536\u7684\u5355\u5c42\u3001\u5185\u5b58\u578b\u7a00\u758fdata\u8f93\u5165\u3002\u4fdd\u7559\u539fRDS\uff0c\u8f93\u51fa\u5230\u65b0\u6587\u4ef6\u3002\u5757\u5927\u5c0f\u662f\u6f14\u793a\u8bbe\u7f6e\uff0c\u5e94\u6839\u636e\u5f53\u524d\u65b9\u6cd5\u548c\u8d44\u6e90\u6d4b\u91cf\u8c03\u6574\uff1b\u5b83\u4e0d\u4ee3\u8868\u6240\u6709ScaleData\u65b9\u6cd5\u90fd\u91c7\u7528\u540c\u6837\u7684\u5206\u5757\u673a\u5236\u3002<\/p>\n<pre><code class=\"language-r\">m &lt;- LayerData(obj, assay=&quot;RNA&quot;, layer=ly)\nif (!inherits(m, &quot;sparseMatrix&quot;)) {\n  stop(&quot;\u6b64\u4f8b\u4ec5\u7528\u4e8e\u5185\u5b58\u578b\u7a00\u758f\u8f93\u5165\uff1b\u6309\u5b9e\u9645\u540e\u7aef\u67e5\u8be2\u5bf9\u5e94\u65b9\u6cd5&quot;)\n}\nrm(m)\nscaled &lt;- ScaleData(obj, assay=&quot;RNA&quot;, features=features,\n                    block.size=500, verbose=TRUE)\nz &lt;- LayerData(scaled, assay=&quot;RNA&quot;, layer=&quot;scale.data&quot;)\nstopifnot(all(features %in% rownames(z)))\nprint(list(class=class(z), dim=dim(z)))\nsaveRDS(scaled, &quot;rna-scaled-selected-features.rds&quot;)<\/code><\/pre>\n<h2>\u4e3a\u4ec0\u4e48\u51cf\u5c0f\u5757\u4ecd\u53ef\u80fd\u5931\u8d25<\/h2>\n<p>\u964d\u4f4e\u5355\u6b21\u8ba1\u7b97\u5757\u53ef\u80fd\u51cf\u5c11\u90e8\u5206\u4e34\u65f6\u5360\u7528\uff0c\u4f46\u6700\u7ec8\u7ed3\u679c\u3001\u539f\u5bf9\u8c61\u3001\u56de\u5f52\u6570\u636e\u548c\u5176\u4ed6\u4e34\u65f6\u5bf9\u8c61\u4ecd\u9700\u5b58\u653e\u3002\u5b83\u4e0d\u80fd\u5c06\u4e00\u4e2a\u65b9\u6cd5\u4e0a\u5fc5\u987b\u5b8c\u6574\u8f93\u51fa\u7684\u5de8\u5927\u77e9\u9635\u81ea\u52a8\u53d8\u6210\u6d41\u5f0f\u7ed3\u679c\u3002\u5916\u5c42\u7528\u8fdb\u7a0b\u8d44\u6e90\u5de5\u5177\u8bb0\u5f55\u5cf0\u503c\uff0cR\u7684object.size\u53ea\u8f85\u52a9\u7406\u89e3\u8fd4\u56de\u5bf9\u8c61\u4f53\u79ef\uff0c\u4e24\u8005\u4e0d\u80fd\u6df7\u7528\u3002<\/p>\n<p>\u5982\u679c\u66f4\u6362\u5757\u5927\u5c0f\u540c\u65f6\u66f4\u6362\u7279\u5f81\u96c6\u5408\u6216\u56de\u5f52\u53d8\u91cf\uff0c\u5c31\u4e0d\u80fd\u628a\u5dee\u5f02\u90fd\u5f52\u56e0\u4e8e\u5206\u5757\u3002\u6bcf\u6b21\u53ea\u6539\u53d8\u4e00\u4e2a\u56e0\u7d20\uff0c\u4fdd\u7559\u76f8\u540c\u8f93\u5165\u548c\u8f93\u51fa\u8981\u6c42\u3002\u907f\u514d\u7528\u66f4\u6fc0\u8fdb\u7684\u8fc7\u6ee4\u89e3\u51b3\u6280\u672f\u95ee\u9898\u540e\uff0c\u53c8\u628a\u7ed3\u679c\u5ba3\u79f0\u4e3a\u539f\u4efb\u52a1\u7684\u7b49\u4ef7\u5b8c\u6210\u3002<\/p>\n<h2>\u9a8c\u6536\u540e\u518d\u51b3\u5b9a\u662f\u5426\u6269\u5bb9<\/h2>\n<p>\u6838\u5bf9scale.data\u7684\u7279\u5f81\u4e0e\u7ec6\u80de\u96c6\u5408\u3001\u77e9\u9635\u7c7b\u548c\u540e\u7eedPCA\u80fd\u5426\u8bfb\u53d6\u6240\u9700\u7279\u5f81\u3002\u5fc5\u8981\u65f6\u62bd\u53d6\u5c0f\u8303\u56f4\u6838\u67e5\u6570\u503c\u662f\u5426\u6709\u9650\uff0c\u5e76\u91cd\u65b0\u8f7d\u5165\u4fdd\u5b58\u6587\u4ef6\u3002\u4e0d\u8981\u5728\u5927\u77e9\u9635\u4e0a\u989d\u5916\u751f\u6210\u6574\u5757\u903b\u8f91\u526f\u672c\uff0c\u53ea\u4e3a\u505a\u4e00\u6b21\u6ca1\u6709\u5206\u5757\u7684\u5168\u91cf\u68c0\u67e5\u3002<\/p>\n<h2>\u4e2d\u5fc3\u5316\u4e3a\u4f55\u53ef\u80fd\u6539\u53d8\u7a00\u758f\u6027<\/h2>\n<p>\u5bf9\u4e00\u4e2a\u5747\u503c\u5927\u4e8e\u96f6\u7684\u7279\u5f81\uff0c\u539f\u672c\u4e3a\u96f6\u7684\u6761\u76ee\u5728\u51cf\u53bb\u5747\u503c\u540e\u5c31\u53ef\u80fd\u53d8\u6210\u975e\u96f6\u503c\uff0c\u56e0\u6b64\u4e0d\u80fd\u4ececounts\u91cc\u96f6\u5f88\u591a\u63a8\u65ad\u7f29\u653e\u540e\u4ecd\u7136\u8282\u7701\u540c\u6837\u7a7a\u95f4\u3002\u5177\u4f53\u5b9e\u73b0\u53ef\u4ee5\u91c7\u7528\u4e0d\u540c\u5b58\u50a8\u6216\u5ef6\u8fdf\u8ba1\u7b97\u7b56\u7565\uff0c\u6240\u4ee5\u5e94\u540c\u65f6\u68c0\u67e5\u6570\u5b66\u64cd\u4f5c\u548c\u5b9e\u9645\u540e\u7aef\uff0c\u800c\u4e0d\u662f\u53ea\u770b\u8f93\u5165\u7c7b\u540d\u3002<\/p>\n<p>\u96f6\u65b9\u5dee\u7279\u5f81\u3001\u975e\u5e38\u5c11\u7684\u6709\u6548\u89c2\u6d4b\u6216\u534f\u53d8\u91cf\u95ee\u9898\uff0c\u8fd8\u53ef\u80fd\u5f71\u54cd\u6570\u503c\u548c\u6a21\u578b\u3002\u53d1\u73b0\u5f02\u5e38\u65f6\uff0c\u5148\u6309\u65b9\u6cd5\u6838\u5bf9\u6570\u636e\uff0c\u4e0d\u4ee5\u589e\u52a0\u5185\u5b58\u66ff\u4ee3\u8fd9\u4e9b\u5224\u65ad\u3002\u6570\u503c\u62bd\u67e5\u53ef\u4ee5\u5206\u5c0f\u8303\u56f4\u5b8c\u6210\uff0c\u907f\u514d\u4e3a\u4e00\u6b21\u5168\u91cf\u68c0\u67e5\u518d\u751f\u6210\u540c\u5c3a\u5bf8\u4e34\u65f6\u5bf9\u8c61\u3002<\/p>\n<p>\u7279\u5f81\u8303\u56f4\u548c\u5b9e\u73b0\u5df2\u7ecf\u5408\u7406\u3001\u5fc5\u8981\u8ba1\u7b97\u4ecd\u8d85\u8fc7\u53ef\u7528\u5185\u5b58\u65f6\uff0c\u53ef\u4ee5\u6574\u7406\u7f29\u653e\u7279\u5f81\u6570\u3001\u7ec6\u80de\u6570\u3001\u77e9\u9635\u7c7b\u3001\u56de\u5f52\u9879\u4e0e\u5cf0\u503c\u65e5\u5fd7\uff0c\u8bc4\u4f30\u5927\u5185\u5b58\u72ec\u4eabCPU\u79df\u8d41\u3002\u82e5\u6839\u56e0\u662f\u9519\u8bef\u5c42\u6216\u65b9\u6cd5\u4e0d\u517c\u5bb9\uff0c\u5e94\u5148\u4fee\u590d\uff0c\u518d\u6d4b\u91cf\u8d44\u6e90\u3002<\/p>\n<h2>\u53c2\u8003\u4e0e\u7248\u672c\u6838\u5bf9<\/h2>\n<ul>\n<li><a href=\"https:\/\/satijalab.org\/seurat\/reference\/scaledata\">Seurat\uff1aScaleData\u53c2\u8003<\/a><\/li>\n<li><a href=\"https:\/\/satijalab.org\/seurat\/reference\/sctransform\">Seurat\uff1aSCTransform\u53c2\u8003<\/a><\/li>\n<li><a href=\"https:\/\/satijalab.org\/seurat\/articles\/bpcells_interaction_vignette.html\">Seurat\uff1aBPCells\u78c1\u76d8\u77e9\u9635\u4e0e\u4fdd\u5b58<\/a><\/li>\n<li><a href=\"https:\/\/stat.ethz.ch\/R-manual\/R-devel\/library\/base\/html\/Memory-limits.html\">R\u5b98\u65b9\u624b\u518c\uff1a\u5185\u5b58\u9650\u5236\u4e0e\u5bf9\u8c61<\/a><\/li>\n<\/ul>\n<section class=\"ysx-tutorial-downloads\">\n<h2>\u4e0b\u8f7d\u7ec3\u4e60\u6750\u6599<\/h2>\n<p><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/rental-tutorials-400\/rental_q016_a\/023e950f3ad269a0aeb2\/rental_q016_a.pdf\" download>\u4e0b\u8f7d\u6559\u7a0b PDF<\/a> \u00b7 <a href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/rental-tutorials-400\/rental_q016_a\/9f1705c4f2fad2b5eb34\/rental_q016_a.zip\" download>\u4e0b\u8f7d\u4ee3\u7801\u4e0e\u793a\u4f8b\u6570\u636e ZIP<\/a><\/p>\n<p>\u5148\u6838\u5bf9\u5305\u5185SHA256\u6e05\u5355\uff0c\u518d\u5728\u72ec\u7acb\u76ee\u5f55\u590d\u73b0\u3002<\/p>\n<\/section>\n<section class=\"ysx-rental-recommendation\">\n<h2>\u9700\u8981\u989d\u5916\u7b97\u529b\u65f6<\/h2>\n<p>\u9002\u7528\u524d\u63d0\uff1a\u7f29\u5c0f\u5230\u5408\u7406\u7279\u5f81\u96c6\u540e\u4ecd\u4e0d\u80fd\u6ee1\u8db3\u5206\u6790\u9700\u6c42\u3002\u5982\u9700\u989d\u5916\u8d44\u6e90\uff0c\u53ef\u8bc4\u4f30\u5927\u5185\u5b58\u72ec\u4eabCPU\u670d\u52a1\u5668\u79df\u8d41\u3002\u54a8\u8be2\u65f6\u8bf4\u660e\u8f6f\u4ef6\u7248\u672c\u3001\u4efb\u52a1\u89c4\u6a21\u548c\u4f7f\u7528\u671f\u9650\uff0c\u914d\u7f6e\u4e0e\u652f\u6301\u8303\u56f4\u53e6\u884c\u786e\u8ba4\u3002<\/p>\n<details>\n<summary>\u54a8\u8be2\u672c\u4efb\u52a1\u7684\u670d\u52a1\u5668\u914d\u7f6e<\/summary>\n<p>    <section class=\"brtc-section\" id=\"rental-consultation\" aria-labelledby=\"brtc-title-66555\">\n    <h2 id=\"brtc-title-66555\">\u54a8\u8be2\u672c\u4efb\u52a1\u7684\u670d\u52a1\u5668\u914d\u7f6e<\/h2>\n    <p>\u7559\u4e0b\u624b\u673a\u53f7\uff0c\u6211\u4eec\u4f1a\u7ed3\u5408\u672c\u6587\u4efb\u52a1\u4e0e\u60a8\u6c9f\u901a\u670d\u52a1\u5668\u914d\u7f6e\u548c\u62a5\u4ef7\u3002<\/p>\n    <form class=\"brtc-form\" method=\"post\" action=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-admin\/admin-ajax.php\" data-article-key=\"rental_q016_a\">\n    <input type=\"hidden\" name=\"action\" value=\"brtc_submit\"><input type=\"hidden\" name=\"post_id\" value=\"66555\">\n    <input type=\"hidden\" name=\"article_key\" value=\"rental_q016_a\"><input type=\"hidden\" name=\"nonce\" value=\"a07adf1e1b\">\n    <input type=\"hidden\" name=\"request_id\" value=\"92d957eb52d4b91779ab2dbbe449a407\"><input type=\"hidden\" name=\"first_key\" value=\"rental_q016_a\">\n    <label>\u624b\u673a\u53f7<input name=\"phone\" type=\"tel\" inputmode=\"numeric\" autocomplete=\"tel-national\" minlength=\"11\" maxlength=\"11\" pattern=\"1[3-9][0-9]{9}\" required 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