{"id":66567,"date":"2026-11-15T23:15:00","date_gmt":"2026-11-15T15:15:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66567"},"modified":"2026-11-15T14:01:11","modified_gmt":"2026-11-15T06:01:11","slug":"rental-q019-a","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/rental-q019-a\/","title":{"rendered":"\u6d4b\u91cf\u5bf9\u8c61\u5185\u5bb9\u3001\u5b58\u50a8\u683c\u5f0f\u548c\u78c1\u76d8\u8bfb\u5199\u6027\u80fd"},"content":{"rendered":"<p><!-- ysx-rental-campaign:rental-tutorials-400-20261114 article:rental_q019_a --><\/p>\n<div class=\"ysx-rental-tutorial\">\n<figure class=\"ysx-tutorial-figure\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/rental_q019_a-poster.png?resize=1200%2C720&#038;ssl=1\" alt=\"\u5927\u5bf9\u8c61\u8bfb\u5199\u6162\uff0c\u5148\u6d4b\u538b\u7f29\u4e0e\u8def\u5f84\uff1a\u76d8\u70b9\u5bf9\u8c61\u5185\u5bb9\uff1b\u6bd4\u8f83\u4e24\u79cd\u538b\u7f29\u8bbe\u7f6e\uff1b\u65b0\u8fdb\u7a0b\u9a8c\u8bc1\u8bfb\u53d6\uff1b\u6309\u8def\u5f84\u548c\u7f13\u5b58\u89e3\u91ca\u5dee\u5f02\" width=\"1200\" height=\"720\" loading=\"lazy\"><figcaption>\u672c\u6587\u64cd\u4f5c\u4e0e\u68c0\u67e5\u8def\u5f84\u793a\u610f\u3002<\/figcaption><\/figure>\n<p>Seurat\u5bf9\u8c61\u4fdd\u5b58\u6216\u91cd\u65b0\u8bfb\u5165\u5f88\u6162\uff0c\u53ef\u80fd\u6765\u81ea\u5e8f\u5217\u5316\u538b\u7f29\u3001CPU\u3001\u78c1\u76d8\u6216\u7f51\u7edc\u8def\u5f84\u3002\u6587\u4ef6\u5c0f\u5e76\u4e0d\u8bf4\u660e\u8fd0\u884c\u65f6\u5bf9\u8c61\u5c0f\uff1b\u53cd\u8fc7\u6765\uff0cRDS\u53ef\u8bfb\u4e5f\u4e0d\u4fdd\u8bc1\u5bf9\u8c61\u6240\u5f15\u7528\u7684\u5916\u90e8\u77e9\u9635\u76ee\u5f55\u5b58\u5728\u3002\u5148\u628a\u4fdd\u5b58\u65f6\u95f4\u3001\u6587\u4ef6\u5927\u5c0f\u3001\u65b0\u8fdb\u7a0b\u8bfb\u53d6\u65f6\u95f4\u548c\u5185\u5bb9\u5b8c\u6574\u6027\u5206\u5f00\u8bb0\u5f55\uff0c\u518d\u6bd4\u8f83\u65b9\u6848\u3002<\/p>\n<h2>\u5148\u76d8\u70b9\u5bf9\u8c61\u4e0e\u540e\u7aef<\/h2>\n<p>\u8bb0\u5f55\u7ec6\u80de\u548c\u7279\u5f81\u6570\u3001assay\/layer\u3001R\u4e0eSeurat\u7248\u672c\u53ca\u5bf9\u8c61\u662f\u5426\u5f15\u7528\u78c1\u76d8\u77e9\u9635\u3002\u4fdd\u7559\u4e00\u4e2a\u539f\u59cb\u526f\u672c\uff1b\u5728\u65b0\u6d4b\u8bd5\u76ee\u5f55\u6bd4\u8f83\u8bbe\u7f6e\uff0c\u4e0d\u8986\u76d6\u6b63\u5f0f\u6587\u4ef6\u3002object.size\u662f\u5bf9\u8c61\u5927\u5c0f\u7684\u8fd1\u4f3c\u503c\uff0c\u4e0d\u80fd\u4ee3\u8868\u8fdb\u7a0b\u5cf0\u503c\u6216\u5916\u90e8\u76ee\u5f55\u603b\u5927\u5c0f\u3002<\/p>\n<table>\n<thead>\n<tr>\n<th>\u8bb0\u5f55\u9879<\/th>\n<th>\u5982\u4f55\u6d4b<\/th>\n<th>\u4e0d\u80fd\u63a8\u51fa<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\u5bf9\u8c61\u5927\u5c0f<\/td>\n<td>object.size<\/td>\n<td>\u8fdb\u7a0b\u5cf0\u503c<\/td>\n<\/tr>\n<tr>\n<td>\u5e8f\u5217\u5316\u6587\u4ef6<\/td>\n<td>file.info<\/td>\n<td>\u8fd8\u539f\u540e\u7684\u5185\u5b58<\/td>\n<\/tr>\n<tr>\n<td>\u5199\u5165\/\u8bfb\u53d6<\/td>\n<td>\u8ba1\u65f6\u5e76\u8bb0\u5f55\u8def\u5f84<\/td>\n<td>\u6362\u538b\u7f29\u4e00\u5b9a\u66f4\u5feb<\/td>\n<\/tr>\n<tr>\n<td>\u5bf9\u8c61\u7ed3\u6784<\/td>\n<td>\u7ef4\u5ea6\u3001assay\u548clayer<\/td>\n<td>\u5916\u90e8\u6570\u636e\u9f50\u5168<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u6bd4\u8f83\u538b\u7f29\u8bbe\u7f6e<\/h2>\n<p>\u5bf9\u76f8\u540c\u5bf9\u8c61\u5199\u5165\u4e0d\u540c\u7684\u65b0\u6587\u4ef6\uff0c\u8bb0\u5f55\u5199\u5165\u65f6\u95f4\u548c\u5927\u5c0f\u3002\u6d4b\u8bd5\u6587\u4ef6\u9700\u8981\u4e34\u65f6\u7a7a\u95f4\uff0c\u7a7a\u95f4\u4e0d\u8db3\u65f6\u4e0d\u8981\u540c\u65f6\u521b\u5efa\u591a\u4efd\u5927\u526f\u672c\uff1b\u5148\u89c4\u5212\u76ee\u5f55\u3002\u538b\u7f29\u53ef\u80fd\u51cf\u5c11\u78c1\u76d8\u7528\u91cf\u3001\u589e\u52a0CPU\u5de5\u4f5c\uff0c\u7ed3\u8bba\u53ea\u5bf9\u8fd9\u4efd\u5bf9\u8c61\u548c\u5f53\u524d\u5b58\u50a8\u73af\u5883\u6709\u6548\u3002<\/p>\n<pre><code class=\"language-r\">stopifnot(exists(\"obj\"))\ndir.create(\"rds-test\", showWarnings=FALSE)\nstopifnot(!file.exists(\"rds-test\/plain.rds\"),\n          !file.exists(\"rds-test\/gzip.rds\"))\nt_plain &lt;- system.time(saveRDS(obj,\"rds-test\/plain.rds\",compress=FALSE))\nt_gzip &lt;- system.time(saveRDS(obj,\"rds-test\/gzip.rds\",compress=\"gzip\"))\nprint(rbind(plain=t_plain,gzip=t_gzip))\nprint(file.info(c(\"rds-test\/plain.rds\",\"rds-test\/gzip.rds\"))$size)\nwriteLines(capture.output(sessionInfo()),\"rds-test\/session.txt\")<\/code><\/pre>\n<h2>\u5728\u65b0R\u8fdb\u7a0b\u4e2d\u9a8c\u8bc1\u5185\u5bb9<\/h2>\n<p>\u5355\u72ec\u8fd0\u884c\u8bfb\u53d6\u811a\u672c\uff0c\u907f\u514d\u539f\u4f1a\u8bdd\u5bf9\u8c61\u5360\u7528\u63a9\u76d6\u5b9e\u9645\u8f7d\u5165\u6210\u672c\u3002\u5148\u68c0\u67e5\u662f\u5426\u6210\u529f\u8bfb\u5165\uff0c\u518d\u6bd4\u5bf9\u5206\u6790\u5fc5\u9700\u7684assay\u3001layer\u3001\u7ec6\u80de\/\u7279\u5f81\u7ef4\u5ea6\u548c\u6837\u672c\u6807\u8bc6\uff1b\u6709\u78c1\u76d8\u540e\u7aef\u65f6\uff0c\u8fd8\u8981\u8bfb\u4e00\u5c0f\u5757\u77e9\u9635\u6570\u636e\uff0c\u786e\u8ba4\u5916\u90e8\u8def\u5f84\u771f\u5b9e\u53ef\u7528\u3002<\/p>\n<pre><code class=\"language-r\">args &lt;- commandArgs(trailingOnly=TRUE)\nstopifnot(length(args)==1L)\nt &lt;- system.time(x &lt;- readRDS(args[1]))\nprint(t); print(class(x)); print(dim(x))\nif (inherits(x,\"Seurat\")) {\n  print(SeuratObject::Assays(x))\n  print(lapply(SeuratObject::Assays(x), function(a) SeuratObject::Layers(x[[a]])))\n}\nprint(format(object.size(x),units=\"GiB\"))\nwriteLines(capture.output(sessionInfo()),\"read-session.txt\")<\/code><\/pre>\n<p>\u82e5\u6587\u4ef6\u53ef\u8bfb\u4f46\u8bbf\u95ee layer \u62a5\u8def\u5f84\u9519\u8bef\uff0c\u5e94\u628a\u5b83\u5f53\u4f5c\u540e\u7aef\u8fc1\u79fb\u95ee\u9898\u3002SeuratObject\u63d0\u4f9bSaveSeuratRds\u4e0eLoadSeuratRds\u5904\u7406\u78c1\u76d8layer\uff0c\u5176move\u548crelative\u9009\u9879\u4f1a\u6539\u53d8\u6587\u4ef6\u7ec4\u7ec7\u6216\u8def\u5f84\u884c\u4e3a\uff0c\u5148\u67e5\u770b\u5f53\u524d\u7248\u672c\u6587\u6863\u5e76\u5728\u526f\u672c\u9a8c\u8bc1\u3002RDS\u7528\u4e8e\u6062\u590dR\u5bf9\u8c61\uff0c\u4e0d\u5e94\u76f4\u63a5\u5f53\u6210\u901a\u7528\u7684\u8de8\u8f6f\u4ef6\u4ea4\u6362\u683c\u5f0f\u3002<\/p>\n<h2>\u533a\u5206CPU\u3001\u7f13\u5b58\u548cI\/O<\/h2>\n<p>\u4fdd\u5b58\u65f6CPU\u5fd9\u3001\u78c1\u76d8\u4e0d\u5fd9\uff0c\u53ef\u80fd\u8bf4\u660e\u538b\u7f29\u5f00\u9500\u503c\u5f97\u6bd4\u8f83\uff1b\u7f51\u7edc\u76d8\u8bfb\u53d6\u6162\uff0c\u5148\u5728\u5141\u8bb8\u7684\u5de5\u4f5c\u76d8\u4f7f\u7528\u540c\u4e00\u6587\u4ef6\u505a\u5bf9\u7167\u3002\u7b2c\u4e00\u6b21\u4e0e\u7b2c\u4e8c\u6b21\u8bfb\u53d6\u53ef\u80fd\u53d7\u7cfb\u7edf\u7f13\u5b58\u5f71\u54cd\uff0c\u5e94\u8bb0\u5f55\u6b21\u6570\u3001\u987a\u5e8f\u3001\u6302\u8f7d\u70b9\u548c\u5176\u4ed6\u4f20\u8f93\u4efb\u52a1\u3002\u4e0d\u8981\u6e05\u5171\u4eab\u7cfb\u7edf\u7f13\u5b58\u6765\u5236\u9020\u201c\u51b7\u8bfb\u201d\u6d4b\u8bd5\uff0c\u4e5f\u4e0d\u8981\u628a\u5185\u5b58\u76d8\u7ed3\u679c\u5f53\u666e\u901a\u78c1\u76d8\u6027\u80fd\u3002<\/p>\n<p>\u6700\u540e\u4fdd\u7559\u9009\u5b9a\u5bf9\u8c61\u3001\u5185\u5bb9\u6e05\u5355\u3001\u8f6f\u4ef6\u7248\u672c\u548c\u6d4b\u91cf\u65e5\u5fd7\u3002\u82e5\u538b\u7f29\u4e0e\u8def\u5f84\u5747\u5df2\u6838\u5bf9\u800c\u5de5\u4f5c\u6d41\u4ecd\u53d7\u6301\u7eed\u8bfb\u5199\u9650\u5236\uff0c\u518d\u51ed\u5bf9\u8c61\u89c4\u6a21\u3001\u8bbf\u95ee\u9891\u7387\u548c\u7528\u6237\u5e76\u53d1\u8bc4\u4f30\u5de5\u4f5c\u76d8\u9700\u6c42\uff1b\u5907\u4efd\u4e0e\u6062\u590d\u8303\u56f4\u9700\u5355\u72ec\u660e\u786e\u3002<\/p>\n<h2>\u5982\u4f55\u5224\u65ad\u6d4b\u8bd5\u7ed3\u679c<\/h2>\n<p>\u5982\u679cgzip\u6587\u4ef6\u660e\u663e\u66f4\u5c0f\u4f46\u5199\u5165\u66f4\u4e45\uff0c\u662f\u5426\u91c7\u7528\u53d6\u51b3\u4e8e\u7a7a\u95f4\u538b\u529b\u548c\u4fdd\u5b58\u9891\u7387\uff1b\u6bcf\u6b21\u4fdd\u5b58\u90fd\u8ba9\u4ea4\u4e92\u5206\u6790\u7b49\u5f85\u65f6\uff0c\u53ef\u6bd4\u8f83\u53e6\u4e00\u79cd\u53d7\u652f\u6301\u7684\u538b\u7f29\u8bbe\u7f6e\uff0c\u5e76\u68c0\u67e5\u65b0\u8fdb\u7a0b\u8bfb\u53d6\u65f6\u95f4\u3002\u4e00\u6b21\u4f1a\u8bdd\u5185\u91cd\u590d\u8fd0\u884c\u53ef\u80fd\u547d\u4e2d\u7f13\u5b58\u6216\u53d7\u5176\u4ed6\u4efb\u52a1\u5f71\u54cd\uff0c\u81f3\u5c11\u8bb0\u5f55\u591a\u6b21\u7ed3\u679c\uff0c\u4e0d\u8981\u53ea\u6311\u6700\u5feb\u7684\u4e00\u6b21\u3002<\/p>\n<p>\u5185\u5bb9\u9a8c\u6536\u9664\u4e86\u5bf9\u8c61\u7ef4\u5ea6\uff0c\u8fd8\u8981\u68c0\u67e5\u5206\u6790\u4f9d\u8d56\u7684\u6837\u672c\u6807\u7b7e\u3001\u5173\u952eassay\/layer\u548c\u964d\u7ef4\u540d\u79f0\u3002\u5982\u679c\u8fd9\u4e9b\u5185\u5bb9\u5728\u5bfc\u51fa\u524d\u5c31\u7f3a\u5931\uff0c\u6210\u529f\u8bfb\u53d6\u4e5f\u4e0d\u4ee3\u8868\u5f52\u6863\u5408\u683c\u3002\u5bf9\u5916\u90e8\u77e9\u9635\u518d\u8bfb\u53d6\u4e00\u4e2a\u5c0f\u5207\u7247\uff0c\u80fd\u53d1\u73b0\u8def\u5f84\u65ad\u5f00\uff1b\u987b\u5728\u8fc1\u79fb\u76ee\u7684\u5730\u6d4b\u8bd5\uff0c\u4e0d\u80fd\u8fd8\u4ece\u539f\u76ee\u5f55\u8bfb\u3002<\/p>\n<p>\u538b\u7f29\u6bd4\u8f83\u65f6\u56fa\u5b9a\u5bf9\u8c61\u3001R\u7248\u672c\u4e0e\u6302\u8f7d\u70b9\uff1b\u6539\u53d8\u7f51\u7edc\u8def\u5f84\u5c31\u53e6\u8bb0\u4e00\u7ec4\u6761\u4ef6\u3002\u5b58\u50a8\u89c4\u5212\u5e94\u63d0\u4f9b\u5bf9\u8c61\u5927\u5c0f\u8303\u56f4\u3001\u6bcf\u5468\u4fdd\u5b58\u548c\u8bfb\u53d6\u6b21\u6570\u3001\u5e76\u53d1\u4f1a\u8bdd\u3001\u5b9e\u6d4b\u8bfb\u5199\u65f6\u95f4\uff0c\u4ee5\u53ca\u78c1\u76d8\u540e\u7aef\u76ee\u5f55\u662f\u5426\u5fc5\u987b\u540c\u65f6\u4fdd\u7559\u3002<\/p>\n<h2>\u53c2\u8003\u8d44\u6599<\/h2>\n<ul>\n<li><a href=\"https:\/\/stat.ethz.ch\/R-manual\/R-devel\/library\/base\/html\/readRDS.html\">R saveRDS\/readRDS\u5b98\u65b9\u624b\u518c<\/a><\/li>\n<li><a href=\"https:\/\/stat.ethz.ch\/R-manual\/R-devel\/library\/utils\/html\/object.size.html\">R object.size\u5b98\u65b9\u53c2\u8003<\/a><\/li>\n<li><a href=\"https:\/\/manpages.debian.org\/bookworm\/time\/time.1.en.html\">GNU time\u624b\u518c\uff08Debian\u53d1\u5e03\uff09<\/a><\/li>\n<\/ul>\n<section class=\"ysx-tutorial-downloads\">\n<h2>\u4e0b\u8f7d\u7ec3\u4e60\u6750\u6599<\/h2>\n<p><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/rental-tutorials-400\/rental_q019_a\/f0fd70acf6e136576ea2\/rental_q019_a.pdf\" download>\u4e0b\u8f7d\u6559\u7a0b PDF<\/a> \u00b7 <a href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/rental-tutorials-400\/rental_q019_a\/c47dab7a7bd09acf44a1\/rental_q019_a.zip\" download>\u4e0b\u8f7d\u4ee3\u7801\u4e0e\u793a\u4f8b\u6570\u636e ZIP<\/a><\/p>\n<p>\u5148\u6838\u5bf9\u5305\u5185SHA256\u6e05\u5355\uff0c\u518d\u5728\u72ec\u7acb\u76ee\u5f55\u590d\u73b0\u3002<\/p>\n<\/section>\n<section class=\"ysx-rental-recommendation\">\n<h2>\u9700\u8981\u989d\u5916\u7b97\u529b\u65f6<\/h2>\n<p>\u9002\u7528\u524d\u63d0\uff1a\u5bf9\u8c61\u7cbe\u7b80\u540e\u4ecd\u6709\u6301\u7eedI\/O\u6216\u5b58\u50a8\u5bb9\u91cf\u9700\u6c42\u3002\u5982\u9700\u989d\u5916\u8d44\u6e90\uff0c\u53ef\u8bc4\u4f30\u670d\u52a1\u5668\u79df\u8d41\u53ca\u6309\u9700\u5de5\u4f5c\u76d8\u6216\u5b58\u50a8\u3002\u54a8\u8be2\u65f6\u8bf4\u660e\u8f6f\u4ef6\u7248\u672c\u3001\u4efb\u52a1\u89c4\u6a21\u548c\u4f7f\u7528\u671f\u9650\uff0c\u914d\u7f6e\u4e0e\u652f\u6301\u8303\u56f4\u53e6\u884c\u786e\u8ba4\u3002<\/p>\n<details>\n<summary>\u54a8\u8be2\u672c\u4efb\u52a1\u7684\u670d\u52a1\u5668\u914d\u7f6e<\/summary>\n<p>    <section class=\"brtc-section\" id=\"rental-consultation\" aria-labelledby=\"brtc-title-66567\">\n    <h2 id=\"brtc-title-66567\">\u54a8\u8be2\u672c\u4efb\u52a1\u7684\u670d\u52a1\u5668\u914d\u7f6e<\/h2>\n    <p>\u7559\u4e0b\u624b\u673a\u53f7\uff0c\u6211\u4eec\u4f1a\u7ed3\u5408\u672c\u6587\u4efb\u52a1\u4e0e\u60a8\u6c9f\u901a\u670d\u52a1\u5668\u914d\u7f6e\u548c\u62a5\u4ef7\u3002<\/p>\n    <form class=\"brtc-form\" method=\"post\" action=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-admin\/admin-ajax.php\" data-article-key=\"rental_q019_a\">\n    <input type=\"hidden\" name=\"action\" value=\"brtc_submit\"><input type=\"hidden\" name=\"post_id\" value=\"66567\">\n    <input type=\"hidden\" name=\"article_key\" value=\"rental_q019_a\"><input type=\"hidden\" name=\"nonce\" value=\"c246e5bdcb\">\n    <input type=\"hidden\" name=\"request_id\" value=\"8758d1122c1b341153a658ee85434e4a\"><input type=\"hidden\" name=\"first_key\" value=\"rental_q019_a\">\n    <label>\u624b\u673a\u53f7<input name=\"phone\" type=\"tel\" inputmode=\"numeric\" autocomplete=\"tel-national\" minlength=\"11\" 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