{"id":66569,"date":"2026-11-15T23:30:00","date_gmt":"2026-11-15T15:30:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66569"},"modified":"2026-11-15T14:01:11","modified_gmt":"2026-11-15T06:01:11","slug":"rental-q019-b","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/rental-q019-b\/","title":{"rendered":"\u6309\u6570\u636e\u8bfb\u5199\u4e60\u60ef\u914d\u7f6e\u5de5\u4f5c\u76d8\u4e0e\u957f\u671f\u4fdd\u5b58\u7a7a\u95f4"},"content":{"rendered":"<p><!-- ysx-rental-campaign:rental-tutorials-400-20261114 article:rental_q019_b --><\/p>\n<div class=\"ysx-rental-tutorial\">\n<figure class=\"ysx-tutorial-figure\"><img data-recalc-dims=\"1\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/rental_q019_b-poster.png?resize=1200%2C720&#038;ssl=1\" alt=\"\u6309\u5bf9\u8c61\u751f\u547d\u5468\u671f\u914d\u7f6e\u5de5\u4f5c\u76d8\u4e0e\u4fdd\u5b58\u7a7a\u95f4\uff1a\u533a\u5206\u8f93\u5165\u5de5\u4f5c\u4e0e\u7ed3\u679c\uff1b\u6838\u7b97\u540c\u65f6\u5b58\u5728\u7684\u526f\u672c\uff1b\u8bb0\u5f55\u8bfb\u5199\u9891\u7387\u548c\u5e76\u53d1\uff1b\u7ea6\u5b9a\u4fdd\u5b58\u4e0e\u6062\u590d\u8d23\u4efb\" width=\"1200\" height=\"720\" loading=\"lazy\"><figcaption>\u672c\u6587\u64cd\u4f5c\u4e0e\u68c0\u67e5\u8def\u5f84\u793a\u610f\u3002<\/figcaption><\/figure>\n<p>\u89c4\u5212Seurat\u5f52\u6863\u7a7a\u95f4\u65f6\uff0c\u4e0d\u80fd\u53ea\u770b\u4e00\u4efdRDS\u3002\u9879\u76ee\u53ef\u80fd\u540c\u65f6\u4fdd\u7559\u539f\u59cb\u8f93\u5165\u3001\u5de5\u4f5c\u5bf9\u8c61\u3001\u68c0\u67e5\u70b9\u3001\u6700\u7ec8\u7ed3\u679c\u53ca\u4e34\u65f6\u6587\u4ef6\uff1b\u82e5\u78c1\u76d8layer\u5f15\u7528\u5916\u90e8\u76ee\u5f55\uff0c\u90a3\u4e2a\u76ee\u5f55\u4e5f\u662f\u5bf9\u8c61\u53ef\u7528\u6027\u7684\u4e00\u90e8\u5206\u3002\u5148\u5217\u6587\u4ef6\u751f\u547d\u5468\u671f\u548c\u6062\u590d\u76ee\u6807\uff0c\u518d\u4f30\u7b97\u540c\u4e00\u65f6\u95f4\u771f\u6b63\u5e76\u5b58\u7684\u5bb9\u91cf\u3002<\/p>\n<h2>\u5217\u51fa\u6587\u4ef6\u53ca\u751f\u547d\u5468\u671f<\/h2>\n<p>\u628a\u539f\u59cb\u8f93\u5165\u3001\u53c2\u8003\u6570\u636e\u3001\u5de5\u4f5c\u5bf9\u8c61\u3001\u68c0\u67e5\u70b9\u3001\u6700\u7ec8\u4ea4\u4ed8\u548c\u53ef\u91cd\u5efa\u7f13\u5b58\u5206\u5f00\u767b\u8bb0\u3002\u8bb0\u5f55\u6bcf\u9879\u5927\u5c0f\u3001\u76ee\u5f55\u3001\u4ea7\u751f\u6b65\u9aa4\u3001\u4fdd\u7559\u671f\u9650\u548c\u662f\u5426\u53ef\u91cd\u5efa\u3002\u4e0d\u8981\u628a\u4e2d\u95f4\u6587\u4ef6\u81ea\u52a8\u5f53\u5783\u573e\u5220\u9664\uff1a\u5148\u786e\u8ba4\u6062\u590d\u6d41\u7a0b\u7528\u4e0d\u5230\u5b83\uff0c\u518d\u7531\u9879\u76ee\u8d1f\u8d23\u4eba\u51b3\u5b9a\u6e05\u7406\u3002<\/p>\n<table>\n<thead>\n<tr>\n<th>\u7c7b\u522b<\/th>\n<th>\u8bb0\u5f55<\/th>\n<th>\u8fc1\u79fb\u91cd\u70b9<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\u8f93\u5165\/\u53c2\u8003<\/td>\n<td>\u7248\u672c\u3001\u6821\u9a8c\u503c\u4e0e\u6765\u6e90<\/td>\n<td>\u91cd\u73b0\u6240\u9700\u6587\u4ef6\u5b8c\u6574<\/td>\n<\/tr>\n<tr>\n<td>\u5de5\u4f5c\u5bf9\u8c61<\/td>\n<td>RDS\u53ca\u5916\u90e8layer\u76ee\u5f55<\/td>\n<td>\u76f8\u5bf9\u8def\u5f84\u548c\u6743\u9650<\/td>\n<\/tr>\n<tr>\n<td>\u68c0\u67e5\u70b9<\/td>\n<td>\u6b65\u9aa4\u3001\u53c2\u6570\u548c\u53ef\u590d\u7528\u6027<\/td>\n<td>\u4e0e\u811a\u672c\u7248\u672c\u5bf9\u5e94<\/td>\n<\/tr>\n<tr>\n<td>\u6700\u7ec8\u7ed3\u679c<\/td>\n<td>\u9a8c\u6536\u6807\u51c6\u4e0e\u4ea4\u4ed8\u76ee\u5f55<\/td>\n<td>\u65b0\u4f4d\u7f6e\u80fd\u91cd\u65b0\u6253\u5f00<\/td>\n<\/tr>\n<tr>\n<td>\u7f13\u5b58\/\u4e34\u65f6<\/td>\n<td>\u53ef\u91cd\u5efa\u4f9d\u636e\u4e0e\u671f\u9650<\/td>\n<td>\u6e05\u7406\u524d\u786e\u8ba4\u4e0d\u518d\u4f9d\u8d56<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u6309\u540c\u65f6\u5b58\u5728\u7684\u6700\u5927\u503c\u4f30\u7b97<\/h2>\n<p>\u793a\u4f8b\uff1a\u8f93\u5165200 GiB\u3001\u5de5\u4f5c\u5bf9\u8c6160 GiB\u3001\u68c0\u67e5\u70b960 GiB\u3001\u4e34\u65f6\u76ee\u5f5580 GiB\u3001\u7ed3\u679c40 GiB\uff0c\u82e5\u8fd9\u4e9b\u786e\u5b9e\u540c\u65f6\u5b58\u5728\u4e14\u4f4d\u4e8e\u540c\u4e00\u6587\u4ef6\u7cfb\u7edf\uff0c\u5219\u9700\u7ea6440 GiB\uff0c\u53e6\u7559\u589e\u957f\u4f59\u91cf\u3002\u82e5\u5de5\u4f5c\u5bf9\u8c61\u53ea\u662f\u5f15\u7528\u540c\u4e00\u4e2a\u8f93\u5165\u76ee\u5f55\uff0c\u6216\u76ee\u5f55\u4f4d\u4e8e\u4e0d\u540c\u6302\u8f7d\u70b9\uff0c\u5e94\u6309\u771f\u5b9e\u5e03\u5c40\u91cd\u65b0\u8ba1\u7b97\uff0c\u907f\u514d\u91cd\u590d\u8ba1\u6570\u6216\u8bef\u628a\u5176\u4ed6\u76d8\u7684\u4f59\u91cf\u52a0\u8fdb\u6765\u3002<\/p>\n<pre><code class=\"language-bash\">du -sh input objects checkpoints temporary results\ndf -h .\ndf -i .<\/code><\/pre>\n<p>\u7a7a\u95f4\u4f30\u7b97\u8fd8\u8981\u8986\u76d6\u6587\u4ef6\u7cfb\u7edf\u914d\u989d\u3001inode\u548c\u5199\u76d8\u671f\u95f4\u53ef\u80fd\u5e76\u5b58\u7684\u65b0\u65e7\u7248\u672c\u3002\u538b\u7f29\u5305\u6216\u5907\u4efd\u5236\u4f5c\u65f6\uff0c\u6e90\u4e0e\u76ee\u6807\u901a\u5e38\u4f1a\u540c\u65f6\u5360\u7a7a\u95f4\uff1b\u5916\u90e8\u78c1\u76d8\u77e9\u9635\u4e5f\u53ef\u80fd\u4e0d\u5728RDS\u76ee\u5f55\u6240\u5728\u6302\u8f7d\u70b9\u3002<\/p>\n<h2>\u8fc1\u79fb\u5305\u8981\u5305\u542b\u5916\u90e8\u77e9\u9635<\/h2>\n<p>\u666e\u901asaveRDS\u4fdd\u5b58R\u5bf9\u8c61\uff1bSeurat\u78c1\u76d8layer\u53ef\u80fd\u662f\u8def\u5f84\u5f15\u7528\u3002\u5bf9BPCells\u7b49\u540e\u7aef\uff0c\u5e94\u6309SeuratObject\u5f53\u524d\u7684SaveSeuratRds\u8bf4\u660e\u51b3\u5b9amove\u548crelative\u884c\u4e3a\uff0c\u6253\u5305RDS\u4e0e\u540e\u7aef\u76ee\u5f55\u53ca\u5fc5\u8981\u4f9d\u8d56\u3002\u590d\u5236\u540e\u68c0\u67e5\u76ee\u5f55\u6743\u9650\uff0c\u4e0d\u80fd\u53ea\u6821\u9a8cRDS\u6587\u4ef6\u5b58\u5728\u3002<\/p>\n<pre><code class=\"language-r\">library(SeuratObject)\nobj &lt;- LoadSeuratRds(\"bundle\/project.rds\")\nprint(Assays(obj))\nfor (a in Assays(obj)) {\n  print(Layers(obj[[a]]))\n  for (ly in Layers(obj[[a]])) {\n    m &lt;- LayerData(obj, assay=a, layer=ly)\n    print(list(assay=a, layer=ly, dim=dim(m)))\n  }\n}\nwriteLines(capture.output(sessionInfo()),\"bundle\/session.txt\")<\/code><\/pre>\n<p>\u5728\u8fc1\u79fb\u76ee\u6807\u542f\u52a8\u65b0\u7684R\u4f1a\u8bdd\uff0c\u6267\u884c\u91cd\u8f7d\u548c\u5c0f\u8303\u56f4\u77e9\u9635\u8bfb\u53d6\uff0c\u62bd\u67e5\u6837\u672c\u6807\u8bc6\u3001\u7ec6\u80de\u6570\u53ca\u5fc5\u9700layer\u3002\u751f\u6210\u5e26\u76f8\u5bf9\u8def\u5f84\u7684\u5f52\u6863\u65f6\uff0c\u79fb\u52a8\u6574\u5957\u76ee\u5f55\u540e\u518d\u9a8c\u6536\uff1b\u7edd\u5bf9\u8def\u5f84\u4ecd\u6307\u5411\u65e7\u4f4d\u7f6e\u7684\u5bf9\u8c61\u4e0d\u7b97\u8fc1\u79fb\u6210\u529f\u3002<\/p>\n<h2>\u6f14\u7ec3\u6062\u590d\uff0c\u518d\u51b3\u5b9a\u5bb9\u91cf<\/h2>\n<p>\u628a\u4e00\u6b21\u6062\u590d\u6f14\u7ec3\u8bb0\u4e3a\u8f93\u5165\u6e05\u5355\u3001\u76ee\u6807\u8def\u5f84\u3001\u6821\u9a8c\u503c\u3001\u5bf9\u8c61\u91cd\u8f7d\u7ed3\u679c\u548c\u5931\u8d25\u8bb0\u5f55\u3002\u53ea\u6709\u80fd\u4ece\u5f52\u6863\u4f4d\u7f6e\u6062\u590d\u5230\u7ea6\u5b9a\u68c0\u67e5\u70b9\uff0c\u5907\u4efd\u624d\u6ee1\u8db3\u8be5\u9879\u76ee\u7684\u6062\u590d\u76ee\u6807\u3002\u539f\u59cb\u6570\u636e\u3001\u5bf9\u8c61\u548c\u7ed3\u679c\u6d89\u53ca\u53d7\u9650\u6837\u672c\u65f6\uff0c\u76ee\u5f55\u6743\u9650\u4e0e\u5171\u4eab\u8303\u56f4\u4e5f\u8981\u7eb3\u5165\u4ea4\u63a5\u3002<\/p>\n<p>\u63d0\u4ea4\u78c1\u76d8\u9700\u6c42\u65f6\u63d0\u4f9b\u5e76\u5b58\u6587\u4ef6\u6e05\u5355\u3001\u5b9e\u9645\u6302\u8f7d\u70b9\u3001\u6700\u5927\u7a7a\u95f4\u3001\u589e\u957f\u901f\u5ea6\u3001\u5e76\u53d1\u5199\u5165\u548c\u4fdd\u7559\u5468\u671f\u3002\u8fd9\u6837\u624d\u80fd\u533a\u5206\u5bb9\u91cf\u4e0d\u8db3\u3001I\/O\u7b49\u5f85\u3001\u540e\u7aef\u8def\u5f84\u5931\u6548\u548c\u6743\u9650\u95ee\u9898\uff0c\u5e76\u6309\u9879\u76ee\u751f\u547d\u5468\u671f\u5b89\u6392\u5de5\u4f5c\u76d8\u4e0e\u5f52\u6863\u7a7a\u95f4\u3002<\/p>\n<h2>\u5199\u4e00\u4efd\u53ef\u68c0\u67e5\u7684\u5f52\u6863\u6e05\u5355<\/h2>\n<p>\u6bcf\u6b21\u4ea4\u4ed8\u8bb0\u5f55\u76f8\u5bf9\u8def\u5f84\u3001\u6587\u4ef6\u5927\u5c0f\u3001\u4fee\u6539\u65f6\u95f4\u3001SHA256\u3001\u6587\u4ef6\u7528\u9014\u4e0e\u6765\u6e90\u7248\u672c\u3002\u751f\u6210\u6e05\u5355\u540e\u5728\u76ee\u6807\u4f4d\u7f6e\u91cd\u65b0\u8ba1\u7b97\u54c8\u5e0c\uff0c\u6bd4\u8f83\u6587\u4ef6\u662f\u5426\u5b8c\u6574\uff1b\u54c8\u5e0c\u4e00\u81f4\u53ea\u80fd\u8bc1\u660e\u5b57\u8282\u76f8\u540c\uff0c\u4ecd\u8981\u542f\u52a8R\u3001\u52a0\u8f7d\u5bf9\u8c61\u5e76\u8bfb\u5916\u90e8layer\uff0c\u68c0\u67e5\u8f6f\u4ef6\u4f9d\u8d56\u548c\u6743\u9650\u3002\u6e05\u5355\u5e94\u4fdd\u5b58\u5728\u5f52\u6863\u76ee\u5f55\u65c1\uff0c\u5e76\u53e6\u5b58\u4e8e\u9879\u76ee\u8bb0\u5f55\u4e2d\u3002<\/p>\n<pre><code class=\"language-bash\">find bundle -type f -print0 | sort -z | xargs -0 sha256sum &gt; bundle\/SHA256SUMS.txt\n(cd restored_bundle &amp;&amp; sha256sum -c SHA256SUMS.txt)<\/code><\/pre>\n<p>\u8de8\u5e73\u53f0\u8def\u5f84\u5927\u5c0f\u5199\u3001\u7b26\u53f7\u94fe\u63a5\u548c\u5171\u4eab\u6743\u9650\u4e5f\u53ef\u80fd\u5f71\u54cd\u6062\u590d\u3002\u53d7\u9650\u4eba\u7c7b\u6570\u636e\u8981\u786e\u8ba4\u8bbf\u95ee\u63a7\u5236\u548c\u4fdd\u7559\u671f\u9650\uff1b\u8fc1\u79fb\u6d4b\u8bd5\u5e94\u4f7f\u7528\u72ec\u7acb\u76ee\u7684\u76ee\u5f55\uff0c\u4e0d\u8981\u4ee5\u8986\u76d6\u65b9\u5f0f\u540c\u6b65\u552f\u4e00\u5f52\u6863\u3002<\/p>\n<h2>\u533a\u5206\u6062\u590d\u76ee\u6807\u548c\u51b7\u5907\u4efd<\/h2>\n<p>\u7ecf\u5e38\u91cd\u8dd1\u7684\u5de5\u4f5c\u5bf9\u8c61\u9002\u5408\u53ef\u5feb\u901f\u8bfb\u53d6\u7684\u5de5\u4f5c\u76d8\uff1b\u957f\u671f\u4fdd\u7559\u7684\u539f\u59cb\u6570\u636e\u548c\u9a8c\u6536\u7ed3\u679c\u53ef\u80fd\u9002\u5408\u5f52\u6863\u5b58\u50a8\u3002\u4e24\u8005\u8bfb\u53d6\u9891\u7387\u548c\u6062\u590d\u65f6\u95f4\u76ee\u6807\u4e0d\u540c\uff0c\u4e0d\u80fd\u4ec5\u6bd4\u8f83\u6bcfGiB\u4ef7\u683c\u3002\u538b\u7f29\u5305\u4e0e\u539f\u76ee\u5f55\u5728\u6253\u5305\u8fc7\u7a0b\u4e2d\u4f1a\u540c\u65f6\u5360\u7a7a\u95f4\uff0c\u5386\u53f2\u7248\u672c\u4e5f\u8981\u8ba1\u5165\u5e76\u5b58\u5cf0\u503c\uff1b\u5176\u5b83\u6302\u8f7d\u70b9\u7684\u7a7a\u4f59\u7a7a\u95f4\u4e0d\u80fd\u5408\u8ba1\u4e3a\u5f53\u524d\u76ee\u5f55\u53ef\u7528\u503c\u3002<\/p>\n<h2>\u53c2\u8003\u8d44\u6599<\/h2>\n<ul>\n<li><a href=\"https:\/\/stat.ethz.ch\/R-manual\/R-devel\/library\/base\/html\/readRDS.html\">R saveRDS\/readRDS\u5b98\u65b9\u624b\u518c<\/a><\/li>\n<li><a href=\"https:\/\/download.samba.org\/pub\/rsync\/rsync.1\">rsync\u5b98\u65b9\u624b\u518c<\/a><\/li>\n<li><a href=\"https:\/\/manpages.debian.org\/bookworm\/coreutils\/sha256sum.1.en.html\">sha256sum\u624b\u518c\uff08Debian\u53d1\u5e03\uff09<\/a><\/li>\n<\/ul>\n<section class=\"ysx-tutorial-downloads\">\n<h2>\u4e0b\u8f7d\u7ec3\u4e60\u6750\u6599<\/h2>\n<p><a href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/rental-tutorials-400\/rental_q019_b\/9a58bfe414b3754a4a43\/rental_q019_b.pdf\" download>\u4e0b\u8f7d\u6559\u7a0b PDF<\/a> \u00b7 <a href=\"https:\/\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/rental-tutorials-400\/rental_q019_b\/40c396091781c7331a2a\/rental_q019_b.zip\" download>\u4e0b\u8f7d\u4ee3\u7801\u4e0e\u793a\u4f8b\u6570\u636e ZIP<\/a><\/p>\n<p>\u5148\u6838\u5bf9\u5305\u5185SHA256\u6e05\u5355\uff0c\u518d\u5728\u72ec\u7acb\u76ee\u5f55\u590d\u73b0\u3002<\/p>\n<\/section>\n<section class=\"ysx-rental-recommendation\">\n<h2>\u9700\u8981\u989d\u5916\u7b97\u529b\u65f6<\/h2>\n<p>\u9002\u7528\u524d\u63d0\uff1a\u5bf9\u8c61\u7cbe\u7b80\u540e\u4ecd\u6709\u6301\u7eedI\/O\u6216\u5b58\u50a8\u5bb9\u91cf\u9700\u6c42\u3002\u5982\u9700\u989d\u5916\u8d44\u6e90\uff0c\u53ef\u8bc4\u4f30\u670d\u52a1\u5668\u79df\u8d41\u53ca\u6309\u9700\u5de5\u4f5c\u76d8\u6216\u5b58\u50a8\u3002\u54a8\u8be2\u65f6\u8bf4\u660e\u8f6f\u4ef6\u7248\u672c\u3001\u4efb\u52a1\u89c4\u6a21\u548c\u4f7f\u7528\u671f\u9650\uff0c\u914d\u7f6e\u4e0e\u652f\u6301\u8303\u56f4\u53e6\u884c\u786e\u8ba4\u3002<\/p>\n<details>\n<summary>\u54a8\u8be2\u672c\u4efb\u52a1\u7684\u670d\u52a1\u5668\u914d\u7f6e<\/summary>\n<p>    <section class=\"brtc-section\" id=\"rental-consultation\" aria-labelledby=\"brtc-title-66569\">\n    <h2 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maxlength=\"11\" pattern=\"1[3-9][0-9]{9}\" required placeholder=\"\u8bf7\u8f93\u516511\u4f4d\u624b\u673a\u53f7\" title=\"\u8bf7\u8f93\u5165\u6709\u6548\u768411\u4f4d\u624b\u673a\u53f7\"><\/label>\n    <div class=\"brtc-honeypot\" aria-hidden=\"true\"><label>\u7f51\u7ad9<input name=\"website\" tabindex=\"-1\" autocomplete=\"off\"><\/label><\/div>\n    <label class=\"brtc-consent\"><input type=\"checkbox\" name=\"consent\" value=\"1\" required>\u6211\u540c\u610f\u901a\u8fc7\u6b64\u624b\u673a\u53f7\u8054\u7cfb\u6211\uff0c\u6c9f\u901a\u672c\u6b21\u670d\u52a1\u5668\u914d\u7f6e\u4e0e\u79df\u8d41\u9700\u6c42\u3002<\/label>\n    <button type=\"submit\">\u63d0\u4ea4\u54a8\u8be2<\/button><p class=\"brtc-status\" role=\"status\" aria-live=\"polite\"><\/p>\n    <\/form>\n    <p class=\"brtc-alternative\">\u4e5f\u53ef<a href=\"https:\/\/work.weixin.qq.com\/kfid\/kfcb05492f29d4d6064\" data-ysx-event=\"contact_click\">\u8054\u7cfb\u4e91\u751f\u4fe1\u4f01\u4e1a\u5fae\u4fe1\u5ba2\u670d<\/a>\uff0c\u8bf4\u660e\u672c\u6587\u4efb\u52a1\u3002<\/p>\n    <\/section>\n    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