{"id":66995,"date":"2026-11-14T19:39:02","date_gmt":"2026-11-14T11:39:02","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=66995"},"modified":"2026-11-14T19:39:02","modified_gmt":"2026-11-14T11:39:02","slug":"mmseqs2-protein-search-clustering-guide","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/mmseqs2-protein-search-clustering-guide\/","title":{"rendered":"MMseqs2 \u641c\u5230\u4e00\u4e2a 100% \u4e00\u81f4\u7684\u86cb\u767d\uff0c\u4e3a\u4ec0\u4e48\u8fd8\u8981\u770b\u8986\u76d6\u5ea6\uff1f"},"content":{"rendered":"
\n

\u5047\u8bbe\u4f60\u4ece\u5b8f\u57fa\u56e0\u7ec4\u91cc\u9884\u6d4b\u51fa\u4e00\u6761 400 \u4e2a\u6c28\u57fa\u9178\u7684\u86cb\u767d\uff0c\u60f3\u5728\u53c2\u8003\u5e93\u91cc\u627e\u7ebf\u7d22\u3002\u641c\u7d22\u5f97\u5230\u4e24\u4e2a\u5019\u9009\uff1a\u4e00\u4e2a\u6709 90 \u4e2a\u6c28\u57fa\u9178\u4e0e\u67e5\u8be2\u5e8f\u5217\u5b8c\u5168\u76f8\u540c\uff1b\u53e6\u4e00\u4e2a\u7684\u4e00\u81f4\u7387\u53ea\u6709 77.6%\uff0c\u5374\u51e0\u4e4e\u4ece\u5934\u5bf9\u5230\u5c3e\u3002\u8be5\u5148\u770b\u54ea\u4e2a\uff1f\u5982\u679c\u8981\u628a\u6574\u4e2a\u86cb\u767d\u5e93\u53bb\u5197\u4f59\uff0c\u8fd9\u4e09\u6761\u5e8f\u5217\u53c8\u8be5\u4e0d\u8be5\u5f52\u5230\u4e00\u7ec4\uff1f<\/p>\n

\u8fd9\u6b63\u597d\u662f MMseqs2 \u7684\u4e24\u79cd\u7528\u9014\u3002\u641c\u7d22<\/strong>\u8ba9\u4f60\u62ff\u7740\u4e00\u6761\u6216\u4e00\u6279\u67e5\u8be2\u5e8f\u5217\uff0c\u627e\u5230\u53c2\u8003\u5e93\u91cc\u7684\u5339\u914d\u5bf9\u8c61\uff0c\u5e76\u68c0\u67e5\u6bcf\u4e00\u5bf9\u6bd4\u5bf9\u8bc1\u636e\u3002\u805a\u7c7b<\/strong>\u4ece\u4e00\u6279\u5f85\u6574\u7406\u7684\u5e8f\u5217\u51fa\u53d1\uff0c\u6839\u636e\u8bbe\u5b9a\u7684\u76f8\u4f3c\u7a0b\u5ea6\u548c\u8986\u76d6\u8303\u56f4\uff0c\u628a\u5b83\u4eec\u5f52\u7ec4\u5e76\u9009\u51fa\u4ee3\u8868\u5e8f\u5217\u3002\u4e0b\u9762\u7528\u540c\u4e00\u7ec4\u4eba\u5de5\u5e8f\u5217\u8dd1\u4e00\u904d\uff0c\u4e24\u79cd\u7ed3\u679c\u5c31\u5bb9\u6613\u8bfb\u61c2\u4e86\u3002MMseqs2 \u5b98\u65b9\u4ed3\u5e93\u4e0e\u672c\u6587\u4f7f\u7528\u7684\u7248\u672c 18-8cc5c<\/a><\/p>\n

\u5927\u5e93\u91cc\u600e\u6837\u627e\u5230\u503c\u5f97\u7ec6\u6bd4\u7684\u5e8f\u5217\uff1f<\/h2>\n

\u8bbe\u60f3\u628a\u67e5\u8be2\u86cb\u767d\u548c\u5e93\u91cc\u6bcf\u4e00\u6761\u86cb\u767d\u90fd\u4ece\u5934\u505a\u4e00\u904d\u5e26\u7f3a\u53e3\u7684\u5c40\u90e8\u6bd4\u5bf9\u3002\u5373\u4f7f\u4e24\u6761\u5e8f\u5217\u51e0\u4e4e\u6beb\u65e0\u5173\u8054\uff0c\u4e5f\u5f97\u4ed8\u51fa\u4e00\u6b21\u6bd4\u5bf9\u7684\u8ba1\u7b97\u4ee3\u4ef7\u3002MMseqs2 \u5e38\u89c4 CPU \u86cb\u767d\u641c\u7d22\u7684\u505a\u6cd5\u662f\u5148\u7528\u6210\u672c\u8f83\u4f4e\u7684\u7ebf\u7d22\u7f29\u5c0f\u5019\u9009\u8303\u56f4\uff0c\u518d\u628a\u7cbe\u7ec6\u6bd4\u5bf9\u7559\u7ed9\u6709\u5e0c\u671b\u7684\u5e8f\u5217\u5bf9\u3002MMseqs2 \u65b9\u6cd5\u8bba\u6587<\/a><\/p>\n

\u7b2c\u4e00\u6761\u7ebf\u7d22\u662f \u77ed\u8bcd<\/strong>\uff0c\u5373\u7531\u82e5\u5e72\u6c28\u57fa\u9178\u7ec4\u6210\u7684 k-mer\u3002\u7a0b\u5e8f\u5229\u7528\u76ee\u6807\u5e93\u7684\u7d22\u5f15\uff0c\u67e5\u627e\u67e5\u8be2\u5e8f\u5217\u4e2d\u77ed\u8bcd\u53ca\u5176\u76f8\u4f3c\u77ed\u8bcd\u51fa\u73b0\u7684\u4f4d\u7f6e\u3002\u5141\u8bb8\u76f8\u4f3c\u6c28\u57fa\u9178\u66ff\u6362\u5f88\u91cd\u8981\uff1a\u54ea\u6015\u4e24\u4e2a\u86cb\u767d\u4fdd\u7559\u4e86\u76f8\u8fd1\u7684\u7ed3\u6784\uff0c\u5176\u4e2d\u4e00\u4e9b\u6b8b\u57fa\u4e5f\u53ef\u80fd\u5df2\u7ecf\u6539\u53d8\u3002<\/p>\n

\u7b2c\u4e8c\u6761\u7ebf\u7d22\u662f\u4f4d\u7f6e\u662f\u5426\u5bf9\u5f97\u4e0a<\/strong>\u3002\u5047\u8bbe\u67e5\u8be2\u7684\u7b2c 10 \u4f4d\u548c\u7b2c 30 \u4f4d\u5404\u6709\u4e00\u4e2a\u77ed\u8bcd\uff0c\u5206\u522b\u547d\u4e2d\u540c\u4e00\u76ee\u6807\u7684\u7b2c 50 \u4f4d\u548c\u7b2c 70 \u4f4d\u3002\u4e24\u6b21\u547d\u4e2d\u7684\u4f4d\u7f6e\u504f\u79fb\u90fd\u662f 40\uff0c\u5728\u6bd4\u5bf9\u77e9\u9635\u91cc\u5c31\u843d\u5728\u540c\u4e00\u6761\u5bf9\u89d2\u7ebf\u4e0a\u3002\u76f8\u6bd4\u5b64\u7acb\u547d\u4e2d\uff0c\u8fd9\u66f4\u503c\u5f97\u6cbf\u7740\u8be5\u65b9\u5411\u505a\u4e00\u6b21\u65e0\u7f3a\u53e3\u8bc4\u5206\u3002\u901a\u8fc7\u9884\u7b5b\u7684\u5019\u9009\u518d\u8fdb\u5165\u5141\u8bb8\u63d2\u5165\u548c\u7f3a\u5931\u7684\u5c40\u90e8\u6bd4\u5bf9\uff0c\u5f97\u5230\u6bd4\u5bf9\u533a\u95f4\u3001\u5f97\u5206\u548c E \u503c\u3002\u9884\u7b5b\u662f\u542f\u53d1\u5f0f\u7684\uff1b\u5f88\u8fdc\u7684\u540c\u6e90\u5e8f\u5217\u53ef\u80fd\u8fdb\u4e0d\u4e86\u5019\u9009\u540d\u5355\u3002\u82e5\u672c\u6765\u5c31\u5728\u627e\u8fdc\u7f18\u6210\u5458\uff0c\u53ef\u4ee5\u63d0\u9ad8\u641c\u7d22\u7075\u654f\u5ea6\u53c2\u6570 -s<\/code>\uff0c\u518d\u89c2\u5bdf\u65b0\u589e\u5019\u9009\u53ca\u5176\u6bd4\u5bf9\u8bc1\u636e\u3002\u5b98\u65b9\u624b\u518c\u4e2d\u7684\u9884\u7b5b\u4e0e\u641c\u7d22\u539f\u7406<\/a><\/p>\n

\u8bfb\u4e00\u5f20\u771f\u5b9e\u8f93\u51fa\u8868\uff1a\u4e00\u81f4\u7387\u7684\u5206\u6bcd\u662f\u8c01\uff1f<\/h2>\n

\u4e3a\u8ba9\u6570\u5b57\u6709\u51fa\u5904\uff0c\u6211\u4eec\u6784\u9020\u4e86\u4e00\u6761 400 \u4e2a\u6c28\u57fa\u9178\u7684\u67e5\u8be2\u5e8f\u5217 Q\uff0c\u4ee5\u53ca\u4e09\u4e2a\u76ee\u6807\u3002\u957f\u76ee\u6807 full_length<\/code> \u7531 Q \u66ff\u6362\u90e8\u5206\u6b8b\u57fa\u5f97\u5230\uff1b\u77ed\u76ee\u6807 domain_only<\/code> \u76f4\u63a5\u622a\u53d6 Q \u7684\u4e00\u6bb5\u8fde\u7eed\u5e8f\u5217\uff1b\u7b2c\u4e09\u6761\u662f\u72ec\u7acb\u751f\u6210\u7684\u968f\u673a\u5e8f\u5217\u3002\u77ed\u76ee\u6807\u7684\u540d\u5b57\u7528\u6765\u6a21\u62df\u201c\u53ea\u5339\u914d\u4e00\u4e2a\u5c40\u90e8\u533a\u57df\u201d\u7684\u573a\u666f\uff0c\u8fd9\u4e9b\u4eba\u5de5\u5e8f\u5217\u6ca1\u6709\u5b9e\u9a8c\u529f\u80fd\u6ce8\u91ca\u3002\u4e0b\u9762\u662f\u8fd9\u6b21\u5c0f\u4f8b\u5b50\u5b9e\u9645\u4f7f\u7528\u7684\u641c\u7d22\u547d\u4ee4\uff0c\u8f93\u5165\u4e3a\u86cb\u767d FASTA\u3002<\/p>\n

mmseqs easy-search query.fa reference.fa hits.tsv tmp_search \\\r\n  --threads 2 --alignment-mode 3 \\\r\n  --format-output "query,target,pident,alnlen,qlen,tlen,qcov,tcov,evalue,bits"\r\n<\/code><\/pre>\n
\n\n\n\n\n\n\n
\u547d\u4e2d\u76ee\u6807<\/th>\n\u4e00\u81f4\u7387 pident<\/th>\n\u6bd4\u5bf9\u957f\u5ea6<\/th>\n\u67e5\u8be2\u8986\u76d6 qcov<\/th>\n\u76ee\u6807\u8986\u76d6 tcov<\/th>\nE \u503c<\/th>\n\u6bd4\u7279\u5f97\u5206<\/th>\n<\/tr>\n<\/thead>\n
full_length<\/td>\n77.6%<\/td>\n399<\/td>\n0.998<\/td>\n0.998<\/td>\n6.366E\u2212229<\/td>\n652<\/td>\n<\/tr>\n
domain_only<\/td>\n100.0%<\/td>\n90<\/td>\n0.225<\/td>\n1.000<\/td>\n1.949E\u221258<\/td>\n196<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"MMseqs2<\/p>\n
--alignment-mode 3<\/code> \u8ba9\u7a0b\u5e8f\u76f4\u63a5\u6309\u6bd4\u5bf9\u8ba1\u7b97\u4e00\u81f4\u7387\u3002pident \u7684\u5206\u6bcd\u662f\u5c40\u90e8\u6bd4\u5bf9\u7684\u5217\u6570\uff0c\u542b\u5185\u90e8\u7f3a\u53e3\u5217\u3002<\/strong> \u7b2c\u4e8c\u884c\u8868\u793a\u8fd9\u6bb5\u6bd4\u5bf9\u4e2d\u7684 90 \u4e2a\u6b8b\u57fa\u5168\u90e8\u76f8\u540c\uff1b\u7528 90 \u9664\u4ee5\u67e5\u8be2\u5168\u957f 400\uff0c\u5f97\u5230\u7684\u67e5\u8be2\u8986\u76d6\u5ea6\u53ea\u6709 0.225\u3002\u76ee\u6807\u6070\u597d\u53ea\u6709 90 \u4e2a\u6b8b\u57fa\uff0c\u6240\u4ee5\u540c\u4e00\u884c\u7684\u76ee\u6807\u8986\u76d6\u5ea6\u662f 1.000\u3002\u8986\u76d6\u5ea6\u5728\u8f93\u51fa\u4e2d\u7528 0 \u5230 1 \u7684\u5c0f\u6570\u8868\u793a\u3002<\/p>\n
E \u503c\u56de\u7b54\u53e6\u4e00\u4e2a\u95ee\u9898\uff1a\u5728\u5f53\u524d\u641c\u7d22\u7a7a\u95f4\u7684\u968f\u673a\u80cc\u666f\u4e0b\uff0c\u9884\u671f\u4f1a\u51fa\u73b0\u591a\u5c11\u4e2a\u5f97\u5206\u81f3\u5c11\u8fd9\u4e48\u9ad8\u7684\u5076\u7136\u5339\u914d\uff1f\u5b83\u4e0e\u6570\u636e\u5e93\u89c4\u6a21\u6709\u5173\u3002\u6bd4\u7279\u5f97\u5206\u8d8a\u9ad8\uff0c\u8868\u793a\u6bd4\u5bf9\u5f97\u5206\u8d8a\u5f3a\u3002\u5728\u672c\u4f8b\u4e2d\uff0c\u4e24\u6761\u547d\u4e2d\u90fd\u6709\u5f88\u5c0f\u7684 E \u503c\uff0c\u4f46\u8fd9\u5e76\u6ca1\u6709\u628a 90 \u4e2a\u6b8b\u57fa\u7684\u5c40\u90e8\u8bc1\u636e\u5ef6\u4f38\u5230\u5176\u4f59 310 \u4e2a\u4f4d\u7f6e\u3002\u5b98\u65b9\u8f93\u51fa\u5b57\u6bb5\u4e0e\u4e00\u81f4\u7387\u5b9a\u4e49<\/a><\/p>\n
\u5982\u679c\u76ee\u6807\u662f\u627e\u6574\u6761\u86cb\u767d\u7684\u529f\u80fd\u5019\u9009<\/strong>\uff0c\u4f1a\u4f18\u5148\u68c0\u67e5\u957f\u76ee\u6807\u7684\u6ce8\u91ca\u6765\u6e90\u3001\u7ed3\u6784\u57df\u6392\u5217\u548c\u5173\u952e\u6b8b\u57fa\u3002\u5982\u679c\u76ee\u6807\u662f\u5b9a\u4f4d\u4e00\u4e2a\u4fdd\u5b88\u7ed3\u6784\u57df<\/strong>\uff0c\u77ed\u547d\u4e2d\u540c\u6837\u503c\u5f97\u4fdd\u7559\uff0c\u8fd8\u5e94\u5bfc\u51fa qstart,qend,tstart,tend<\/code> \u67e5\u770b\u5177\u4f53\u4f4d\u7f6e\u3002\u9762\u5bf9\u5929\u7136\u86cb\u767d\uff0c\u77ed\u547d\u4e2d\u4e5f\u53ef\u80fd\u6765\u81ea\u622a\u65ad\u7684\u57fa\u56e0\u9884\u6d4b\u3002\u4ec5\u51ed\u9996\u4f4d\u547d\u4e2d\uff0c\u65e0\u6cd5\u786e\u5b9a\u4e24\u4e2a\u86cb\u767d\u5177\u6709\u76f8\u540c\u7684\u5e95\u7269\u7279\u5f02\u6027\u3002<\/p>\n
\u7528\u540c\u4e00\u6279\u5e8f\u5217\u505a\u805a\u7c7b\uff0c\u4e3a\u4ec0\u4e48\u77ed\u76ee\u6807\u4f1a\u5355\u72ec\u6210\u7c07\uff1f<\/h2>\n
\u73b0\u5728\u628a Q \u4e0e\u4e09\u4e2a\u76ee\u6807\u5408\u5728\u4e00\u4e2a FASTA \u91cc\u3002\u5047\u8bbe\u76ee\u7684\u53d8\u6210\u6574\u7406\u4e00\u4efd\u5168\u957f\u86cb\u767d\u4ee3\u8868\u96c6<\/strong>\uff0c\u6211\u4eec\u5e0c\u671b\u957f\u5ea6\u76f8\u8fd1\u3001\u4e3b\u4f53\u5e8f\u5217\u76f8\u4f3c\u7684\u86cb\u767d\u8fdb\u5165\u540c\u4e00\u7ec4\uff0c\u540c\u65f6\u4fdd\u7559\u77ed\u7247\u6bb5\u3002\u4e0b\u9762\u662f\u672c\u4f8b\u5b9e\u9645\u8fd0\u884c\u7684\u5efa\u5e93\u3001\u805a\u7c7b\u548c\u6210\u5458\u8868\u5bfc\u51fa\u547d\u4ee4\u3002<\/p>\n
mmseqs createdb proteins.fa proteinsDB\r\nmmseqs cluster proteinsDB clustersDB tmp_members --threads 2 \\\r\n  --min-seq-id 0.7 -c 0.8 --cov-mode 0 --alignment-mode 3\r\nmmseqs createtsv proteinsDB proteinsDB clustersDB members.tsv --threads 1\r\n<\/code><\/pre>\n
\u8fd9\u91cc\u9009\u62e9 70% \u5e8f\u5217\u4e00\u81f4\u7387\u548c 80% \u8986\u76d6\u5ea6\u6765\u6f14\u793a\u5168\u957f\u53bb\u5197\u4f59\uff0c\u4e24\u9879\u9608\u503c\u90fd\u5e94\u968f\u7814\u7a76\u76ee\u7684\u8c03\u6574\u3002--cov-mode 0<\/code> \u8981\u6c42\u6bd4\u5bf9\u540c\u65f6\u5145\u5206\u8986\u76d6\u4e24\u7aef\u3002\u5bf9\u4e8e 400 \u5bf9 90 \u7684\u4f8b\u5b50\uff0c\u5373\u4f7f\u77ed\u5e8f\u5217\u6574\u6bb5\u90fd\u5bf9\u4e0a\uff0c\u6700\u591a\u4e5f\u53ea\u80fd\u8986\u76d6\u957f\u5e8f\u5217\u7684 22.5%\uff0c\u8fbe\u4e0d\u5230\u8fd9\u4e00\u95e8\u69db\u3002\u5b98\u65b9\u8986\u76d6\u6a21\u5f0f\u4e0e\u805a\u7c7b\u8bf4\u660e<\/a><\/p>\n
\n\n\n\n\n\n\n\n
\u672c\u6b21\u4ee3\u8868\u5e8f\u5217<\/th>\n\u6210\u5458\u8868\u4e2d\u5bf9\u5e94\u7684\u6210\u5458<\/th>\n\u600e\u6837\u89e3\u91ca<\/th>\n<\/tr>\n<\/thead>\n
full_length<\/td>\nfull_length\u3001Q<\/td>\n\u957f\u76ee\u6807\u4e0e Q \u7684\u4e3b\u4f53\u5e8f\u5217\u90fd\u5f97\u5230\u8986\u76d6\uff0c\u6ee1\u8db3\u672c\u4f8b\u9608\u503c\u3002\u4ee3\u8868\u672c\u8eab\u4e5f\u51fa\u73b0\u5728\u6210\u5458\u8868\u4e2d\u3002<\/td>\n<\/tr>\n
domain_only<\/td>\ndomain_only<\/td>\n\u77ed\u7247\u6bb5\u5728\u641c\u7d22\u8868\u4e2d\u6709\u663e\u8457\u547d\u4e2d\uff0c\u5728\u5168\u957f\u53bb\u5197\u4f59\u65f6\u4ecd\u88ab\u5355\u72ec\u4fdd\u7559\u3002<\/td>\n<\/tr>\n
unrelated<\/td>\nunrelated<\/td>\n\u5728\u8fd9\u6279\u5e8f\u5217\u548c\u8fd9\u7ec4\u53c2\u6570\u4e0b\uff0c\u5b83\u6ca1\u6709\u4e0e\u5176\u4ed6\u6210\u5458\u5f52\u4e3a\u4e00\u7ec4\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\u539f\u59cb members.tsv<\/code> \u6bcf\u884c\u4e24\u5217\uff0c\u7b2c\u4e00\u5217\u4e3a\u4ee3\u8868 ID\uff0c\u7b2c\u4e8c\u5217\u4e3a\u6210\u5458 ID\u3002\u8981\u7edf\u8ba1\u7c07\u5927\u5c0f\uff0c\u6309\u7b2c\u4e00\u5217\u5206\u7ec4\u8ba1\u6570\u5373\u53ef\u3002\u4ee3\u8868\u662f\u805a\u7c7b\u7b97\u6cd5\u9009\u51fa\u6765\u7684\u5e8f\u5217\uff1b\u5982\u679c\u8981\u7528\u5b83\u505a\u529f\u80fd\u6ce8\u91ca\uff0c\u4ecd\u8981\u6838\u5bf9\u6ce8\u91ca\u8d28\u91cf\u3002\u5c24\u5176\u5728\u771f\u5b9e\u5927\u5e93\u91cc\uff0c\u540c\u7c07\u4e5f\u4e0d\u80fd\u5f53\u4f5c\u201c\u4efb\u610f\u4e24\u6210\u5458\u90fd\u5df2\u9010\u5bf9\u9a8c\u8bc1\u8d85\u8fc7\u9608\u503c\u201d\u7684\u8bc1\u660e\u3002\u60f3\u6bd4\u8f83\u7c07\u5185\u4e24\u6761\u5177\u4f53\u86cb\u767d\uff0c\u5c31\u628a\u5b83\u4eec\u5355\u72ec\u5bf9\u9f50\uff0c\u67e5\u770b\u8986\u76d6\u8303\u56f4\u4e0e\u5dee\u5f02\u4f4d\u7f6e\u3002<\/p>\n
\n\u5c55\u5f00\u793a\u4f8b FASTA\uff0c\u590d\u73b0\u4e24\u5f20\u7ed3\u679c\u8868<\/summary>\n
\u7b2c\u4e00\u6761 Q \u5355\u72ec\u4fdd\u5b58\u4e3a query.fa\uff0c\u4f59\u4e0b\u4e09\u6761\u4fdd\u5b58\u4e3a reference.fa\uff1b\u56db\u6761\u653e\u5728\u4e00\u8d77\u4fdd\u5b58\u4e3a proteins.fa\u3002<\/p>\n
>Q\r\nGMNLNLMFDGSAIKNKVWNFVVLSVYFFTGGASFEPCSCYDDLSLRFVFKTICRCKHLDWTMHIGKLRCWVMMCFADREQSKQNPNPEYFDFTYYDISDKNGGLDVLELIPCICNLSPMPHMILKAFNDITLSHAKSLIVIWREPPNQIRNSSPMFCLLILVVVDFVTFSAIPIVFEKGRQDECYFEGKERMHYMRHKWEYGAITPLAMYTTCEHSNHCHPPWNQAHDGSMRVIKANPYHGYAGPYFKSDAGRMVGSPYSPFKCDPYNGRTYWDIFWAFMHVDMVIITWMRNHIFRVSPHYAQEMTVNYLMCDLSSIWCMGEIRDPQLNGIDMHRRPDQMMKPDWERCMPPQHMWKSCHCDVMLQNQTPRFRKWTGHDGCQTRLTIGAVTYNAWQWAYDG\r\n>domain_only\r\nAFNDITLSHAKSLIVIWREPPNQIRNSSPMFCLLILVVVDFVTFSAIPIVFEKGRQDECYFEGKERMHYMRHKWEYGAITPLAMYTTCEH\r\n>full_length\r\nKMNPDLMFDWSQIWTKVWNFVVLSVYEFTGGASFEPCSPFGDLALGFVFKYIHRMKHLDWTDYIGKLRCWVMMCFADDEQYKENPNPELFCVEYYDISDQNISWDVLELTPFICNVNPMPTMMLKNFNDITLSHAPVLIVIWCEPQNHIRPSSPMFMLLPLVVVDFVNFSAYPIVFEKGAQDECYFEKVERMHYMRHKPEYIAITPLGMTFPCEHSNHCHSNVAQAHDGSMRVVKANPYPGYFGPYVKSDASNVNGSPYSPRKCDPYMGRTYWDIFWCFNEVDMVNITWMCNHEFRVSPHYSQEMTVNYLMCDLSSIWCMGEIRDPQLNGEDMWRRPDQMMKPDWGCCMPPQHMQKSCHTDCMDQNQGPRFRKWTGHDKCQNRLTIGAYTYIAWQWKYDG\r\n>unrelated\r\nIPEPANLWPSLSHQDDWKTVNHHAIPWCLRSNKAWCQPRHIARFWECATTMDKDTWIPFTMGKLGCVDGGLHNWEGTDIAKGLREIHSGGYVAKYMIQCHRMDKWMMGTGFIAYGSPTDLWIHQIGRMVFVCVRFTGWNHCSWQGLSVSFPIKNNMECINIQFSAIKEMCPHQAKKEFNPKQMGLPYVMSTWEPWCLTLEPKEVCLTRVWICNCYNESSCDTPPKDCCDMTYWFIWEATESKKIVFCPPMWSWKCNLTNFEWVPASMYFRQHYFSKGKEFRSETRVCKRQSPWNCYLNASSISERVNHLQIEPNYHYNLLEIKWAHHWYPFAMRDCLKHEKNDYRFFYEICITANATWTRQRPETNRTWTGYVRYMNHGVGTSMYSCDDLFIWATGLNLG<\/code><\/pre>\n<\/details>\n
\u4e0b\u6b21\u62ff\u5230\u4e00\u4e2a\u547d\u4e2d\uff0c\u5148\u95ee\u81ea\u5df1\u8981\u4fdd\u7559\u4ec0\u4e48<\/h2>\n
\u7ed9\u4e00\u6761\u672a\u77e5\u86cb\u767d\u627e\u7ebf\u7d22\uff0c\u4fdd\u5b58\u547d\u4e2d\u5e8f\u5217\u3001\u53cc\u5411\u8986\u76d6\u548c\u6bd4\u5bf9\u5750\u6807<\/strong>\uff0c\u4e4b\u540e\u624d\u6709\u4f9d\u636e\u533a\u5206\u5168\u957f\u5019\u9009\u3001\u5171\u4eab\u7ed3\u6784\u57df\u4e0e\u7247\u6bb5\u3002\u7ed9\u6574\u5e93\u53bb\u5197\u4f59\uff0c\u4fdd\u5b58\u8f93\u5165\u5e93\u7248\u672c\u3001\u805a\u7c7b\u53c2\u6570\u548c\u4ee3\u8868\u2014\u6210\u5458\u6620\u5c04<\/strong>\uff0c\u5426\u5219\u53ea\u7559\u4e0b\u4ee3\u8868 FASTA\uff0c\u4ee5\u540e\u5f88\u96be\u8ffd\u6eaf\u67d0\u6761\u6837\u672c\u5e8f\u5217\u53bb\u4e86\u54ea\u91cc\u3002\u672c\u4f8b\u4e2d\u7684\u4e24\u5f20\u8868\u4e4b\u6240\u4ee5\u7ed9\u51fa\u4e0d\u540c\u7b54\u6848\uff0c\u6b63\u662f\u56e0\u4e3a\u201c\u80fd\u627e\u5230\u5c40\u90e8\u76f8\u4f3c\u201d\u548c\u201c\u9002\u5408\u7528\u4e00\u6761\u5168\u957f\u4ee3\u8868\u66ff\u4ee3\u201d\u662f\u4e24\u4e2a\u4e0d\u540c\u7684\u95ee\u9898\u3002<\/p>\n
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