{"id":67098,"date":"2026-11-14T19:46:16","date_gmt":"2026-11-14T11:46:16","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=67098"},"modified":"2026-11-14T20:48:06","modified_gmt":"2026-11-14T12:48:06","slug":"kraken2-bracken-species-abundance-report-denominators","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/kraken2-bracken-species-abundance-report-denominators\/","title":{"rendered":"\u540c\u4e00\u79cd\u83cc\uff0cKraken \u62a5 2.69%\uff0cBracken \u4e3a\u4ec0\u4e48\u62a5 5.53%\uff1f"},"content":{"rendered":"
\n

\u5b8f\u57fa\u56e0\u7ec4\u5206\u6790\u7ed3\u675f\uff0c\u505a\u7269\u79cd\u7ec4\u6210\u56fe\u7684\u4eba\u4ece Kraken \u62a5\u544a\u91cc\u6284\u4e86\u5927\u80a0\u6746\u83cc\u7684\u767e\u5206\u6bd4\uff0c\u6574\u7406\u8865\u5145\u8868\u7684\u4eba\u7528\u7684\u5374\u662f Bracken \u4e30\u5ea6\u5217\u3002\u4e24\u4e2a\u6570\u5bf9\u4e0d\u4e0a\u3002\u91cd\u65b0\u8dd1\u5206\u7c7b\u4e4b\u524d\uff0c\u53ef\u4ee5\u5148\u7528\u9879\u76ee\u4f5c\u8005\u63d0\u4f9b\u7684\u4e00\u4efd\u62a5\u544a\u6838\u8d26\u3002\u540c\u4e00\u4e2a\u7269\u79cd\u3001\u540c\u4e00\u6279\u8f93\u5165\uff0c\u6cbf\u7740\u5206\u7c7b\u6811\u5f80\u4e0b\u770b\uff0c\u518d\u5f04\u6e05\u4e24\u4e2a\u767e\u5206\u6bd4\u5206\u522b\u9664\u4ee5\u4e86\u4ec0\u4e48\uff0c\u5c31\u80fd\u89e3\u91ca\u8fd9\u9053\u5dee\u989d\u3002<\/p>\n

\u672c\u6587\u590d\u7b97\u4f7f\u7528 Bracken \u4f5c\u8005\u516c\u5f00\u7684 Kraken \u793a\u4f8b\u62a5\u544a\u3001\u914d\u5957\u7684 75 nt \u5206\u5e03\u6587\u4ef6\u548c\u7269\u79cd\u5c42\u7ea7\u7684\u4f30\u7b97\u7a0b\u5e8f\uff0c\u6700\u4f4e\u8ba1\u6570\u53c2\u6570\u8bbe\u4e3a 10\u3002\u793a\u4f8b\u662f\u8f83\u65e9\u7684 Kraken \u62a5\u544a\uff0c\u4e0b\u9762\u8bfb\u5217\u7684\u65b9\u6cd5\u540c\u6837\u9002\u7528\u4e8e Kraken 2 \u9ed8\u8ba4\u7684\u516d\u5217\u62a5\u544a\u3002\u6240\u6709\u6570\u5b57\u6765\u81ea\u8fd9\u6b21\u590d\u7b97\u3002\u8bfb\u8005\u53ef\u4ee5\u7528\u81ea\u5df1\u7684\u6587\u4ef6\u66ff\u6362\u6587\u4e2d\u7684\u8f93\u5165\u3002\u4f5c\u8005\u53d1\u5e03\u7684\u793a\u4f8b\u6587\u4ef6<\/a><\/p>\n

Kraken \u7684\u540c\u4e00\u884c\uff0c\u4e3a\u4ec0\u4e48\u6709\u4e24\u4e2a\u8ba1\u6570<\/h2>\n

\u9ed8\u8ba4\u62a5\u544a\u7684\u524d\u4e09\u5217\u4f9d\u6b21\u8bb0\u5f55\u8be5\u5206\u7c7b\u5206\u652f\u5360\u5168\u90e8\u8f93\u5165\u7247\u6bb5\u7684\u767e\u5206\u6bd4\u3001\u843d\u5728\u8be5\u5206\u652f\u53ca\u5176\u6240\u6709\u540e\u4ee3\u7684\u7247\u6bb5\u6570\u3001\u76f4\u63a5\u5206\u5230\u5f53\u524d\u8282\u70b9\u7684\u7247\u6bb5\u6570\u3002\u63a5\u4e0b\u6765\u7684\u4e09\u5217\u662f\u5206\u7c7b\u5c42\u7ea7\u3001\u5206\u7c7b\u7f16\u53f7\u548c\u540d\u79f0\u3002\u7247\u6bb5\u662f\u672c\u6b21\u5206\u7c7b\u7684\u5355\u4f4d\uff0c\u6210\u5bf9\u6a21\u5f0f\u4e0b\u4e00\u5bf9\u8bfb\u6bb5\u5408\u8ba1\u4e00\u4e2a\u7247\u6bb5\u3002\u5148\u786e\u8ba4\u62ff\u5230\u7684\u662f\u6837\u672c\u7684\u6807\u51c6\u62a5\u544a\u3002\u9010\u6761\u8bfb\u6bb5\u7684\u5206\u7c7b\u8f93\u51fa\u3001\u5e26\u989d\u5916 minimizer \u5217\u7684\u62a5\u544a\u4ee5\u53ca MPA \u98ce\u683c\u62a5\u544a\u7684\u5217\u5e03\u5c40\u90fd\u4e0d\u540c\u3002Kraken 2 \u6837\u672c\u62a5\u544a\u8bf4\u660e<\/a><\/p>\n

\n\n\n\n\n\n\n\n\n
\u793a\u4f8b\u4e2d\u7684\u884c<\/th>\n\u8be5\u5206\u652f\u53ca\u540e\u4ee3<\/th>\n\u76f4\u63a5\u843d\u5728\u672c\u8282\u70b9<\/th>\n\u7b2c\u4e00\u5217\u5360\u6bd4<\/th>\n<\/tr>\n<\/thead>\n
\u672a\u5206\u7c7b<\/td>\n425,595<\/td>\n425,595<\/td>\n1.60%<\/td>\n<\/tr>\n
\u6839\u8282\u70b9<\/td>\n26,241,409<\/td>\n0<\/td>\n98.40%<\/td>\n<\/tr>\n
\u57c3\u5e0c\u83cc\u5c5e\uff0c\u7f16\u53f7 561<\/td>\n717,629<\/td>\n0<\/td>\n2.69%<\/td>\n<\/tr>\n
\u5927\u80a0\u6746\u83cc\uff0c\u7f16\u53f7 562<\/td>\n717,629<\/td>\n449,674<\/td>\n2.69%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\u8fd9\u91cc\u6709\u4e24\u4e2a\u5f88\u5bb9\u6613\u6f0f\u6389\u7684\u4e8b\u5b9e\u3002\u7b2c\u4e00\uff0c\u7269\u79cd\u884c\u4e0a\u7684 717,629 \u5df2\u7ecf\u5305\u542b\u66f4\u7ec6\u5c42\u7ea7\u7684\u7247\u6bb5\uff0c\u76f4\u63a5\u505c\u5728\u79cd\u8282\u70b9\u7684\u53ea\u6709 449,674\u3002\u5176\u4f59 267,955 \u4e2a\u843d\u5230\u4e86\u8fd9\u4e2a\u7269\u79cd\u4ee5\u4e0b\u3002\u7b2c\u4e8c\uff0c\u5c5e\u884c\u4e5f\u663e\u793a 717,629\uff0c\u662f\u56e0\u4e3a\u5b50\u6811\u5411\u4e0a\u6c47\u603b\u3002\u540c\u4e00\u6279\u7247\u6bb5\u4f1a\u540c\u65f6\u51fa\u73b0\u5728\u5c5e\u3001\u79cd\u4ee5\u53ca\u66f4\u9ad8\u5c42\u7684\u7d2f\u8ba1\u6570\u91cc\u3002\u628a\u4e00\u5f20\u5b8c\u6574\u62a5\u544a\u7684\u7b2c\u4e8c\u5217\u76f4\u63a5\u6c42\u548c\uff0c\u7b49\u4e8e\u6cbf\u5206\u7c7b\u6811\u91cd\u590d\u8ba1\u6570\u3002\u8981\u590d\u7b97\u603b\u8f93\u5165\uff0c\u7528\u6839\u5206\u652f\u52a0\u4e0a\u672a\u5206\u7c7b\u5373\u53ef\uff0c\u672c\u4f8b\u662f 26,241,409 \u52a0 425,595\uff0c\u5408\u8ba1 26,667,004\u3002\u7269\u79cd\u5206\u652f\u7684 717,629 \u9664\u4ee5\u8fd9\u4e2a\u603b\u6570\uff0c\u5c31\u5f97\u5230 2.69%\u3002<\/p>\n

\u5982\u679c\u53ea\u60f3\u786e\u8ba4\u67d0\u4e2a\u7269\u79cd\uff0c\u6309\u5206\u7c7b\u7f16\u53f7\u63d0\u53d6\u6bd4\u6309\u540d\u5b57\u6a21\u7cca\u641c\u7d22\u53ef\u9760\u3002\u4e0b\u9762\u7684\u547d\u4ee4\u8bfb\u53d6\u5236\u8868\u7b26\u5206\u9694\u7684\u516d\u5217\u62a5\u544a\uff0c\u5e76\u4fdd\u7559\u6574\u884c\u4f9b\u6838\u5bf9\u3002\u5148\u770b\u6587\u4ef6\u91cc\u662f\u5426\u786e\u5b9e\u6709\u516d\u5217\uff0c\u518d\u628a\u7f16\u53f7\u6362\u6210\u76ee\u6807\u7269\u79cd\u3002\u540d\u79f0\u76f8\u4f3c\u7684\u83cc\u682a\u548c\u4e0a\u7ea7\u5c5e\u5c31\u4e0d\u4f1a\u6df7\u8fdb\u6765\u3002<\/p>\n

awk -F '\\t' 'NF == 6 && $5 == 562 {print}' sample_test.report\r\n<\/code><\/pre>\n
Bracken \u6539\u53d8\u4e86\u5206\u5b50\uff0c\u4e5f\u6539\u53d8\u4e86\u5206\u6bcd<\/h2>\n
\u8fd1\u7f18\u7269\u79cd\u5171\u4eab\u4e00\u90e8\u5206\u5e8f\u5217\u3002\u5206\u7c7b\u5668\u53ef\u4ee5\u628a\u4e00\u6761\u7247\u6bb5\u653e\u5230\u5927\u5bb6\u5171\u6709\u7684\u7956\u5148\u8282\u70b9\uff0c\u5355\u51ed\u5b83\u6682\u65f6\u5206\u4e0d\u6e05\u6765\u81ea\u54ea\u4e2a\u79cd\u3002Bracken \u4f7f\u7528\u4e0e\u5206\u7c7b\u6570\u636e\u5e93\u53ca\u8bfb\u957f\u76f8\u5339\u914d\u7684\u5206\u5e03\u6587\u4ef6\uff0c\u4f30\u8ba1\u4e0a\u5c42\u8282\u70b9\u4e2d\u7684\u4e00\u90e8\u5206\u7247\u6bb5\u8be5\u600e\u6837\u5206\u7ed9\u4e0b\u5c5e\u7269\u79cd\u3002\u8f93\u51fa\u91cc\u7684 added_reads<\/code> \u662f\u6a21\u578b\u91cd\u5206\u914d\u7684\u4f30\u8ba1\u91cf\u3002\u5b83\u4e0d\u4f1a\u4ea7\u751f\u65b0\u7684\u6d4b\u5e8f\u7247\u6bb5\uff0c\u4e5f\u6ca1\u6709\u628a\u6bcf\u6761\u6a21\u7cca\u7247\u6bb5\u9010\u4e00\u8bc1\u5b9e\u5230\u4e86\u67d0\u4e2a\u7269\u79cd\u3002<\/p>\n
\u8fd9\u6b21\u91cd\u7b97\uff0c\u5927\u80a0\u6746\u83cc\u4e00\u884c\u7684 kraken_assigned_reads<\/code> \u662f 717,629\uff0c\u4e0e\u79cd\u5206\u652f\u7d2f\u8ba1\u6570\u4e00\u81f4\u3002\u518d\u52a0 added_reads<\/code> \u7684 732,184\uff0c\u5f97\u5230 new_est_reads<\/code> \u7684 1,449,813\u3002\u5373\u4fbf\u5176\u4e2d\u7684\u5c5e\u8282\u70b9\u76f4\u63a5\u8ba1\u6570\u4e3a\u96f6\uff0c\u66f4\u9ad8\u5c42\u5171\u540c\u7956\u5148\u7684\u7247\u6bb5\u4ecd\u53ef\u80fd\u53c2\u4e0e\u5206\u914d\u3002\u56e0\u6b64\uff0c\u62ff\u201c\u5c5e\u884c\u7b2c\u4e09\u5217\u201d\u5f53\u4f5c\u8be5\u79cd\u6240\u6709\u65b0\u589e\u8ba1\u6570\u7684\u4e0a\u9650\uff0c\u4e5f\u4f1a\u5bf9\u4e0d\u4e0a\u8d26\u3002Bracken \u7684\u4f30\u7b97\u65b9\u6cd5\u4e0e\u53c2\u6570<\/a><\/p>\n
\u73b0\u5728\u53ef\u4ee5\u5bf9\u7167\u4e24\u4e2a\u5206\u6bcd\u4e86\u3002\u82e5\u628a 1,449,813 \u9664\u4ee5\u5168\u90e8\u8f93\u5165\u7247\u6bb5\u6570<\/strong>\uff0c\u7ed3\u679c\u7ea6\u4e3a 5.44%\u3002Bracken \u8868\u91cc\u7684 fraction_total_reads<\/code> \u5219\u662f 0.05525\uff0c\u6362\u6210\u767e\u5206\u6bd4\u7ea6\u4e3a 5.53%\u3002\u4f5c\u8005\u7684\u5b9e\u73b0\u7528\u672c\u6b21\u5728\u76ee\u6807\u5c42\u7ea7\u7eb3\u5165\u4f30\u7b97\u7684\u603b\u91cf\u5f52\u4e00\u5316\u3002\u672c\u4f8b\u7269\u79cd\u5c42\u7ea7\u4f30\u8ba1\u603b\u91cf\u7ea6\u4e3a 2,624 \u4e07\uff0c\u672a\u5206\u7c7b\u7247\u6bb5\u4e0d\u5728\u8fd9\u4e2a\u5206\u6bcd\u91cc\u3002\u6309\u7269\u79cd\u884c\u5bfc\u51fa\u7684\u6574\u6570\u8ba1\u6570\u6216\u820d\u5165\u540e\u7684\u5c0f\u6570\u91cd\u52a0\uff0c\u672b\u4f4d\u53ef\u80fd\u7565\u6709\u51fa\u5165\u3002Bracken \u4e30\u5ea6\u5217\u7684\u8ba1\u7b97\u5b9e\u73b0<\/a><\/p>\n
\"Bracken
\u540c\u4e00\u4efd\u516c\u5f00\u6570\u636e\uff0c\u5148\u6838\u5bf9\u5206\u5b50\u7684\u91cd\u5206\u914d\uff0c\u518d\u6bd4\u8f83\u4e24\u4e2a\u5206\u6bcd\u3002\u56fe\u4e2d\u767e\u5206\u6570\u56db\u820d\u4e94\u5165\u5230\u5c0f\u6570\u70b9\u540e\u4e24\u4f4d\u3002<\/figcaption><\/figure>\n
\u7269\u79cd\u67f1\u5b50\u7ffb\u4e86\u4e00\u500d\uff0c\u5148\u505a\u4e00\u4e2a\u5206\u6bcd\u5b9e\u9a8c<\/h2>\n
\u5047\u8bbe\u53e6\u6709\u4e24\u4efd\u6559\u5b66\u6837\u672c\uff0c\u5404\u6295\u5165\u5341\u4e07\u4e2a\u7247\u6bb5\u3002\u76ee\u6807\u79cd\u5728\u4e24\u6b21\u4f30\u7b97\u4e2d\u7684\u7247\u6bb5\u6570\u90fd\u4e3a\u4e00\u4e07\u3002\u7532\u6709\u516b\u4e07\u4e2a\u7247\u6bb5\u8fdb\u5165\u7269\u79cd\u5c42\u7ea7\u7684\u4f30\u7b97\uff0c\u4e59\u53ea\u6709\u56db\u4e07\u4e2a\uff0c\u6b64\u65f6\u76ee\u6807\u79cd\u5728\u7269\u79cd\u7ec4\u6210\u56fe\u4e0a\u7684\u5360\u6bd4\u5206\u522b\u662f\u767e\u5206\u4e4b\u5341\u4e8c\u70b9\u4e94\u548c\u767e\u5206\u4e4b\u4e8c\u5341\u4e94\u3002\u5982\u679c\u4e24\u5f20\u56fe\u53ea\u5c55\u793a\u7ecf\u8fc7\u5f52\u4e00\u5316\u7684\u5f69\u8272\u67f1\u5b50\uff0c\u4e59\u770b\u8d77\u6765\u6070\u597d\u7ffb\u500d\u3002\u7528\u5168\u90e8\u8f93\u5165\u518d\u7b97\uff0c\u4e24\u8005\u5374\u540c\u4e3a\u767e\u5206\u4e4b\u5341\u3002\u5206\u5b50\u4ece\u5934\u5230\u5c3e\u6ca1\u6709\u589e\u52a0\uff0c\u5dee\u5f02\u53d1\u751f\u5728\u80fd\u8fdb\u5165\u8fd9\u5f20\u7ec4\u6210\u56fe\u7684\u7247\u6bb5\u96c6\u5408\u3002<\/p>\n
\u78b0\u5230\u81ea\u5df1\u7684\u8868\u6709\u7c7b\u4f3c\u53d8\u5316\uff0c\u5148\u6309\u6837\u672c\u5217\u51fa\u603b\u8f93\u5165\u3001\u672a\u5206\u7c7b\u7247\u6bb5\u3001\u53c2\u4e0e\u79cd\u7ea7\u4f30\u7b97\u7684\u603b\u91cf\u548c\u76ee\u6807\u79cd\u7684\u4f30\u8ba1\u6570\u3002\u63a5\u7740\u6311\u672a\u5206\u7c7b\u7387\u9ad8\u7684\u4e00\u4efd\u68c0\u67e5\uff0c\u8bfb\u6bb5\u8d28\u91cf\u3001\u53c2\u8003\u5e93\u662f\u5426\u8986\u76d6\u8be5\u73af\u5883\u3001\u4e0a\u6e38\u662f\u5426\u505a\u8fc7\u4e0d\u540c\u7684\u5bbf\u4e3b\u8fc7\u6ee4\u3002\u82e5\u53ea\u91cd\u65b0\u8fd0\u884c\u4e86\u7269\u79cd\u4e30\u5ea6\u4f30\u7b97\uff0c\u5e94\u4fdd\u7559\u7b2c\u4e00\u6b21\u7684\u5206\u7c7b\u62a5\u544a\uff0c\u8fd9\u6837\u53ef\u4ee5\u628a\u5206\u7c7b\u65f6\u53d1\u751f\u7684\u53d8\u5316\u4e0e\u540e\u7eed\u91cd\u65b0\u5206\u914d\u7684\u53d8\u5316\u5206\u5f00\u8ffd\u67e5\u3002<\/p>\n
\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u5148\u7528\u4e94\u6761\u5e8f\u5217\u770b\u61c2\u6700\u4f4e\u5171\u540c\u7956\u5148\uff0c\u518d\u590d\u7b97\u516c\u5f00\u62a5\u544a<\/h2>\n
\u5728 Ubuntu 24.04 \u4e2d\u5b9e\u9645\u8fd0\u884c Kraken 2.1.3\uff0c\u7528\u4e24\u6761\u4eba\u5de5\u53c2\u8003\u5e8f\u5217\u5efa\u4e86\u4e00\u4e2a\u5c0f\u5e93\u3002\u4e24\u6761\u53c2\u8003\u5404\u6709\u4e00\u6bb5\u5404\u81ea\u72ec\u6709\u7684\u5e8f\u5217\uff0c\u672b\u5c3e\u53e6\u6709\u4e09\u767e\u4e94\u5341\u4e2a\u78b1\u57fa\u5b8c\u5168\u76f8\u540c\u3002\u4ece\u7532\u7684\u72ec\u6709\u533a\u53d6\u4e24\u6761\u8bfb\u6bb5\uff0c\u4ece\u4e59\u7684\u72ec\u6709\u533a\u53d6\u4e00\u6761\uff0c\u4ece\u5171\u6709\u533a\u53d6\u4e00\u6761\uff0c\u518d\u52a0\u4e00\u6761\u72ec\u7acb\u751f\u6210\u7684\u968f\u673a\u8bfb\u6bb5\uff0c\u6bcf\u6761\u4e03\u5341\u4e94\u4e2a\u78b1\u57fa\u3002\u9010\u6761\u8f93\u51fa\u4f9d\u6b21\u843d\u5230\u7532\u79cd\u3001\u7532\u79cd\u3001\u4e59\u79cd\u3001\u5171\u540c\u7684\u5c5e\u3001\u672a\u5206\u7c7b\u3002\u8fd9\u4e2a\u4e94\u6761\u8f93\u5165\u7684\u5b9e\u9a8c\u76f4\u63a5\u8bf4\u660e\uff0c\u5c5e\u8fd9\u4e00\u652f\u4e00\u5171\u6536\u4e86\u56db\u6761\uff0c\u5176\u4e2d\u53ea\u6709\u4e00\u6761\u505c\u7559\u5728\u5c5e\u4e0a\u3002\u5b83\u4eec\u5728\u540c\u4e00\u884c\u663e\u793a\u4e3a\u4e24\u4e2a\u4e0d\u540c\u7684\u8ba1\u6570\u3002<\/p>\n
\u968f\u540e\u5728\u76f8\u540c\u73af\u5883\u5b9e\u9645\u7f16\u8bd1\u4e86 Bracken\uff0c\u4ece\u9879\u76ee\u56fa\u5b9a\u63d0\u4ea4\u4e2d\u53d6\u5b98\u65b9\u6570\u636e\u5e93\u7684\u4e03\u5341\u4e94\u78b1\u57fa\u5206\u5e03\u6587\u4ef6\u548c\u914d\u5957\u62a5\u544a\uff0c\u4f7f\u7528\u7269\u79cd\u5c42\u7ea7\u3001\u9608\u503c\u5341\u8c03\u7528\u5b8c\u6574\u547d\u4ee4\u3002\u7a0b\u5e8f\u811a\u672c\u6807\u793a\u4e3a 3.0.2\uff0c\u5927\u80a0\u6746\u83cc\u4e00\u884c\u7ed9\u51fa\u539f\u59cb\u8ba1\u6570 717,629\uff0c\u91cd\u65b0\u5206\u914d\u589e\u52a0 732,184\uff0c\u5408\u8ba1 1,449,813\uff0c\u8f93\u51fa\u6bd4\u4f8b 0.05525\uff0c\u4e0e\u4e0a\u6587\u7b97\u4f8b\u4e00\u81f4\u3002\u7b2c\u4e00\u6b21\u8c03\u7528\u9047\u5230\u4e00\u4e2a\u80fd\u590d\u73b0\u7684\u5b89\u88c5\u95ee\u9898\uff0c\u8be5\u63d0\u4ea4\u7684\u5916\u5c42\u811a\u672c\u8981\u8c03\u7528\u540d\u4e3a python \u7684\u89e3\u91ca\u5668\uff0c\u800c\u8fd9\u4efd Ubuntu \u53ea\u6709 python3\u3002\u628a\u5206\u6790\u73af\u5883\u5185\u7684 python \u547d\u4ee4\u6307\u5411\u540c\u4e00\u7248\u672c\u7684 Python 3 \u540e\u5373\u53ef\u5b8c\u6210\uff0c\u65e0\u987b\u6539\u52a8\u6570\u636e\u5e93\u3002\u5b9e\u9645\u6392\u9519\u65f6\uff0c\u5148\u770b\u6700\u5148\u51fa\u9519\u7684\u89e3\u91ca\u5668\u6216\u6587\u4ef6\u540d\uff0c\u518d\u53bb\u6000\u7591\u751f\u7269\u5b66\u5206\u6bcd\u3002\u672c\u6b21\u4f7f\u7528\u7684 Bracken \u547d\u4ee4\u5165\u53e3\u53ca\u89e3\u91ca\u5668\u8c03\u7528<\/a><\/p>\n
\u6362\u6210\u81ea\u5df1\u7684\u6837\u672c\uff0c\u5148\u6838\u5bf9\u8fd9\u4e09\u4e2a\u6761\u4ef6<\/h2>\n
\u7b2c\u4e00\uff0c\u770b\u5206\u7c7b\u548c\u4f30\u7b97\u662f\u5426\u7528\u540c\u4e00\u5957\u6570\u636e\u5e93\u3002\u76ee\u5f55\u540d\u76f8\u4f3c\u4e0d\u8db3\u4ee5\u786e\u8ba4\uff0c\u6700\u597d\u4fdd\u7559\u5efa\u5e93\u65e5\u671f\u3001\u7269\u79cd\u8303\u56f4\u548c\u5b9e\u9645\u8c03\u7528\u8def\u5f84\u3002\u7b2c\u4e8c\uff0c\u770b\u5206\u5e03\u6587\u4ef6\u7684\u8bfb\u957f\u662f\u5426\u548c\u5904\u7406\u540e\u7684\u6570\u636e\u76f8\u7b26\u3002\u4e0b\u9762\u7684 75 \u6765\u81ea\u4f5c\u8005\u914d\u5957\u793a\u4f8b\u3002\u5982\u679c\u4f60\u7684\u6587\u5e93\u8bfb\u957f\u4e0d\u540c\uff0c\u8981\u6362\u6210\u4e0e\u4e4b\u5339\u914d\u7684\u8bbe\u7f6e\u548c\u5e93\u6587\u4ef6\u3002\u7b2c\u4e09\uff0c\u68c0\u67e5\u6bd4\u8f83\u7684\u662f\u540c\u4e00\u5c42\u7ea7\u3002\u7528\u79cd\u7ea7\u4f30\u8ba1\u753b\u4e00\u7ec4\u3001\u7528\u5c5e\u7ea7\u5206\u652f\u5360\u6bd4\u753b\u53e6\u4e00\u7ec4\uff0c\u4e24\u8fb9\u5217\u540d\u5373\u4f7f\u90fd\u5199\u201c\u76f8\u5bf9\u4e30\u5ea6\u201d\uff0c\u6570\u503c\u7684\u542b\u4e49\u4e5f\u4e0d\u4e00\u81f4\u3002<\/p>\n
\u5982\u679c\u5df2\u51c6\u5907\u597d\u76f8\u5339\u914d\u7684\u5df2\u5efa\u5e93\u76ee\u5f55\uff0c\u53ef\u7528\u5b98\u65b9\u547d\u4ee4\u884c\u5165\u53e3\u3002\u5148\u628a\u793a\u4f8b\u8def\u5f84\u6362\u6210\u81ea\u5df1\u7684\u6570\u636e\u5e93\u76ee\u5f55\uff0c\u8fd9\u91cc\u6f14\u793a\u79cd\u7ea7\u4f30\u7b97\u3002\u53c2\u6570 -t 10<\/code> \u5f71\u54cd\u4f4e\u8ba1\u6570\u5206\u7c7b\u5355\u5143\u662f\u5426\u8fdb\u5165\u4f30\u7b97\uff0c\u5b83\u4e0d\u662f\u7269\u79cd\u5b58\u5728\u7684\u751f\u7269\u5b66\u5224\u5b9a\u7ebf\u3002\u505a\u591a\u6837\u672c\u6bd4\u8f83\u65f6\uff0c\u628a\u8fd9\u9879\u8bbe\u7f6e\u4e0e\u6570\u636e\u5e93\u3001\u8bfb\u957f\u3001\u5206\u6790\u5c42\u7ea7\u4e00\u5e76\u5199\u5165\u65b9\u6cd5\u8bb0\u5f55\u3002<\/p>\n
DB=\/path\/to\/matched-kraken-db\r\nbracken -d "$DB" -i sample.kreport -o sample.bracken \\\r\n  -r 75 -l S -t 10\r\n<\/code><\/pre>\n
\u6700\u540e\u7559\u4e24\u5217\u53ef\u4ee5\u7701\u53bb\u5f88\u591a\u89e3\u91ca\u3002\u7269\u79cd\u7ec4\u6210\u56fe\u7528 Bracken \u7684\u7269\u79cd\u7ea7\u76f8\u5bf9\u4e30\u5ea6\uff0c\u5e76\u5728\u56fe\u6ce8\u4e2d\u5199\u660e\u5f52\u4e00\u5316\u53e3\u5f84\u3002\u6837\u672c\u8d28\u63a7\u8868\u53e6\u7559 Kraken \u7684\u672a\u5206\u7c7b\u6bd4\u4f8b\u3002\u5c24\u5176\u5728\u4e24\u4e2a\u6837\u672c\u672a\u5206\u7c7b\u6bd4\u4f8b\u76f8\u5dee\u5f88\u5927\u65f6\uff0c\u660e\u663e\u53d8\u9ad8\u7684\u7269\u79cd\u767e\u5206\u6bd4\u53ef\u80fd\u90e8\u5206\u6e90\u81ea\u5206\u6bcd\u53d8\u5316\u3002\u82e5\u7814\u7a76\u95ee\u9898\u662f\u67d0\u79cd\u83cc\u7684\u7edd\u5bf9\u6570\u91cf\u6709\u6ca1\u6709\u589e\u52a0\uff0c\u8fd8\u9700\u8981\u603b\u83cc\u91cf\u3001\u5916\u6e90\u6807\u51c6\u6216\u72ec\u7acb\u5b9a\u91cf\u5b9e\u9a8c\u7b49\u989d\u5916\u8bc1\u636e\u3002\u628a\u76ee\u524d\u80fd\u56de\u7b54\u7684\u95ee\u9898\u5199\u51c6\u786e\uff0c\u6bd4\u518d\u7ed9\u67f1\u72b6\u56fe\u52a0\u4e00\u4f4d\u5c0f\u6570\u66f4\u91cd\u8981\u3002<\/p>\n
\u628a\u8fd9\u4e00\u4efd\u6837\u672c\u7684\u6570\u5b57\u6838\u6e05\uff0c\u518d\u56fa\u5b9a\u6570\u636e\u5e93\u548c\u53c2\u6570\u53bb\u8dd1\u6574\u6279\u961f\u5217\uff0c\u540e\u9762\u7684\u8868\u624d\u53ef\u4ee5\u76f4\u63a5\u6bd4\u8f83\u3002\u5982\u679c\u56e2\u961f\u9700\u8981\u957f\u671f\u4fdd\u7559\u8fd9\u5957\u6570\u636e\u5e93\u3001\u53cd\u590d\u5904\u7406\u65b0\u589e\u6837\u672c\uff0c\u53ef\u4e86\u89e3\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668<\/a>\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
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