{"id":67384,"date":"2026-11-14T20:02:29","date_gmt":"2026-11-14T12:02:29","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=67384"},"modified":"2026-11-14T20:48:19","modified_gmt":"2026-11-14T12:48:19","slug":"starsolo-reads-barcodes-raw-matrix-cell-filtering","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/starsolo-reads-barcodes-raw-matrix-cell-filtering\/","title":{"rendered":"STARsolo \u53ea\u53eb\u51fa\u5f88\u5c11\u7ec6\u80de\uff1f\u4ece\u4e24\u4efd FASTQ \u67e5\u5230\u539f\u59cb\u8ba1\u6570\u77e9\u9635"},"content":{"rendered":"
\n

\u540c\u4e00\u4efd10x Genomics \u5355\u7ec6\u80de\u6587\u5e93\uff0c\u6d4b\u5e8f\u4ea4\u4ed8\u5355\u4e0a\u5199\u7740\u51e0\u5343\u4e2a\u7ec6\u80de\uff0cSTARsolo \u7684\u8fc7\u6ee4\u77e9\u9635\u5374\u53ea\u5269\u51e0\u5341\u5217\u3002\u6b64\u65f6\u76f4\u63a5\u653e\u5bbd\u95e8\u69db\uff0c\u53ef\u80fd\u628a\u6ca1\u6709\u6b63\u786e\u8bfb\u5230\u6761\u5f62\u7801\u7684\u6587\u5e93\u753b\u51fa\u4e00\u5f20\u770b\u4f3c\u6b63\u5e38\u7684\u805a\u7c7b\u56fe\u3002\u66f4\u7701\u4e8b\u7684\u6392\u67e5\u987a\u5e8f\u662f\u5148\u8bc1\u660e\u4e24\u7aef\u8bfb\u6bb5\u5404\u53f8\u5176\u804c\uff0c\u7136\u540e\u786e\u8ba4\u539f\u59cb\u77e9\u9635\u7a76\u7adf\u6709\u6ca1\u6709\u5206\u5b50\uff0c\u6700\u540e\u624d\u8ba8\u8bba\u600e\u6837\u5224\u5b9a\u7ec6\u80de\u3002\u6bcf\u4e00\u6b65\u90fd\u80fd\u7559\u4e0b\u522b\u4eba\u770b\u5f97\u61c2\u7684\u4e2d\u95f4\u7ed3\u679c\u3002<\/p>\n

\u4e0b\u6587\u8ba8\u8bba\u7684\u662f\u5e38\u89c1\u768410x 3\u2032\u57fa\u56e0\u8868\u8fbe\u53cc\u7aef\u6587\u5e93\uff0c\u4e00\u7aef\u662f\u8f6c\u5f55\u672c\u5e8f\u5217\uff0c\u53e6\u4e00\u7aef\u643a\u5e26\u7ec6\u80de\u6761\u5f62\u7801\u548c\u5206\u5b50\u6807\u7b7e\u3002\u5176\u4ed6\u5316\u5b66\u4f53\u7cfb\u30015\u2032 \u6587\u5e93\u6216\u81ea\u5b9a\u4e49\u63a5\u5934\u53ef\u80fd\u5177\u6709\u4e0d\u540c\u7684\u8bfb\u6bb5\u7ed3\u6784\u3002\u5148\u5411\u5efa\u5e93\u65b9\u786e\u8ba4\u6587\u5e93\u7c7b\u578b\u4ee5\u53ca\u6bcf\u6bb5\u5e8f\u5217\u7684\u4f4d\u7f6e\u548c\u957f\u5ea6\uff0c\u518d\u4f7f\u7528\u4e0b\u5217\u4f8b\u5b50\u3002\u53ea\u77e5\u9053\u6587\u4ef6\u540d\u91cc\u542b\u6709\u7b2c\u4e00\u7aef\u3001\u7b2c\u4e8c\u7aef\uff0c\u4ecd\u7136\u4e0d\u591f\u3002<\/p>\n

\u786e\u8ba4\u4f20\u7ed9\u7a0b\u5e8f\u7684\u7b2c\u4e00\u4efd\u6587\u4ef6\u662f\u4ec0\u4e48<\/h2>\n

\u666e\u901a\u53cc\u7aef\u6bd4\u5bf9\u547d\u4ee4\u5e38\u628a\u7b2c\u4e00\u7aef\u5199\u5728\u524d\u9762\u3002STARsolo \u5bf9\u8fd9\u7c7b\u8f93\u5165\u89c4\u5b9a\u7684\u987a\u5e8f\u662f\u8f6c\u5f55\u672c\u7aef\u5728\u524d\uff0c\u6761\u5f62\u7801\u548c\u5206\u5b50\u6807\u7b7e\u7aef\u5728\u540e\u3002\u56e0\u6b64\u5e38\u89c110x 3\u2032\u5e03\u5c40\u4f7f\u7528\u7b2c\u4e8c\u7aef\u6587\u4ef6\u5728\u524d\u3001\u7b2c\u4e00\u7aef\u6587\u4ef6\u5728\u540e\u3002\u5bf9\u4e8e\u591a\u4e2a\u6d4b\u5e8f\u6cf3\u9053\uff0c\u5de6\u8fb9\u5217\u51fa\u7684\u6240\u6709\u8f6c\u5f55\u672c\u6587\u4ef6\u4e0e\u53f3\u8fb9\u7684\u6761\u5f62\u7801\u6587\u4ef6\u8981\u9010\u6cf3\u9053\u6210\u5bf9\u3002\u628a\u4e24\u8fb9\u5206\u522b\u6309\u4e0d\u540c\u7684\u547d\u540d\u89c4\u5219\u6392\u5e8f\uff0c\u4f1a\u9020\u6210\u603b\u8bfb\u6bb5\u6570\u770b\u4f3c\u5408\u7406\u3001\u914d\u5bf9\u8eab\u4efd\u5374\u9519\u4e71\u7684\u95ee\u9898\u3002STARsolo \u4f5c\u8005\u7684\u8f93\u5165\u8bf4\u660e\u4e0e\u547d\u4ee4\u793a\u4f8b<\/a><\/p>\n

\u5148\u968f\u673a\u9009\u4e00\u4efd\u6587\u5e93\uff0c\u770b\u4ea4\u4ed8\u5355\u91cc\u7684\u5efa\u5e93\u8bf4\u660e\uff0c\u518d\u4eba\u5de5\u6838\u5bf9\u540c\u4e00\u6cf3\u9053\u9996\u6279\u8bfb\u6bb5\u7684\u6807\u8bc6\u548c\u957f\u5ea6\u3002\u4e0d\u540c\u547d\u540d\u8f6f\u4ef6\u53ef\u80fd\u5728\u8bfb\u6bb5\u6807\u8bc6\u672b\u5c3e\u9644\u52a0\u7aef\u53f7\uff0c\u6bd4\u8f83\u65f6\u53ef\u53bb\u6389\u6210\u5bf9\u547d\u540d\u7ea6\u5b9a\u4e2d\u7684\u7aef\u53f7\uff0c\u4fdd\u7559\u533a\u5206\u6d4b\u5e8f\u7c07\u7684\u5176\u4f59\u90e8\u5206\u3002\u82e5\u884c\u9996\u6807\u8bc6\u672c\u6765\u5c31\u4e0d\u5c5e\u4e8e\u540c\u4e00\u6279\u7c07\uff0c\u5148\u4fee\u6b63\u914d\u5bf9\u6587\u4ef6\u3002\u8bfb\u957f\u53ef\u4ee5\u63d0\u4f9b\u7ebf\u7d22\uff0c\u8f83\u957f\u7684\u4e00\u7aef\u5e76\u975e\u5728\u6240\u6709\u5b9e\u9a8c\u4e2d\u90fd\u6052\u5b9a\u62c5\u5f53\u8f6c\u5f55\u672c\u7aef\u3002<\/p>\n

# \u5728\u5305\u542b\u540c\u4e00\u6cf3\u9053 R1\u3001R2 \u7684\u76ee\u5f55\u4e2d\u8fd0\u884c\uff0c\u5148\u770b\u5404\u81ea\u7b2c\u4e00\u6761\u8bb0\u5f55\r\ngzip -dc sample_R1.fastq.gz | head -n 4\r\ngzip -dc sample_R2.fastq.gz | head -n 4\r\n# \u5e38\u89c1 10x 3\u2032 \u6587\u5e93\u7684\u8f93\u5165\u7247\u6bb5\uff0c\u987b\u5e76\u5165\u4f60\u81ea\u5df1\u7684 STAR \u547d\u4ee4\r\n# --readFilesIn sample_R2.fastq.gz sample_R1.fastq.gz --readFilesCommand zcat\r\n<\/code><\/pre>\n
\u63a5\u7740\u68c0\u67e5\u5141\u8bb8\u7684\u7ec6\u80de\u6761\u5f62\u7801\u6e05\u5355\u4e0e\u5316\u5b66\u4f53\u7cfb\u662f\u5426\u76f8\u7b26\uff0c\u540c\u65f6\u9010\u9879\u5bf9\u7167\u7ec6\u80de\u6761\u5f62\u7801\u548c\u5206\u5b50\u6807\u7b7e\u7684\u8d77\u59cb\u4f4d\u7f6e\u3001\u957f\u5ea6\u3002\u67d0\u4e2a\u53c2\u6570\u5199\u9519\u4e00\u4e2a\u78b1\u57fa\uff0c\u5207\u51fa\u6765\u7684\u6761\u5f62\u7801\u957f\u5ea6\u4ecd\u53ef\u80fd\u5b8c\u5168\u4e00\u81f4\uff0c\u7ee7\u7eed\u8fd0\u884c\u4e5f\u672a\u5fc5\u62a5\u9519\u3002\u4e00\u4e2a\u6709\u8fa8\u522b\u529b\u7684\u8bd5\u9a8c\u662f\u4ece\u540c\u4e00\u5c0f\u6279\u6210\u5bf9\u8bb0\u5f55\u63d0\u53d6\u9884\u671f\u4f4d\u7f6e\u7684\u6761\u5f62\u7801\uff0c\u67e5\u770b\u5b83\u4eec\u4e0e\u8be5\u7248\u672c\u767d\u540d\u5355\u7684\u7cbe\u786e\u5339\u914d\u72b6\u51b5\u3002\u5b83\u9002\u5408\u6bd4\u8f83\u4e24\u79cd\u5e03\u5c40\u8c01\u5408\u7406\u3002\u6b63\u5f0f\u8ba1\u6570\u8fd8\u53ef\u80fd\u7ea0\u6b63\u90e8\u5206\u6761\u5f62\u7801\u9519\u914d\uff0c\u4e0d\u80fd\u628a\u8fd9\u4e2a\u8bd5\u9a8c\u7684\u7cbe\u786e\u5339\u914d\u7387\u76f4\u63a5\u5f53\u6210\u6700\u7ec8\u6709\u6548\u7387\u3002<\/p>\n
\u7528\u4e09\u5c42\u7edf\u8ba1\u786e\u5b9a\u6545\u969c\u53d1\u751f\u5728\u54ea\u91cc<\/h2>\n
\u4ece\u8f93\u51fa\u76ee\u5f55\u4e2d\u540c\u65f6\u627e\u6bd4\u5bf9\u65e5\u5fd7\u3001\u9009\u5b9a\u7279\u5f81\u7c7b\u578b\u4e0b\u7684\u539f\u59cb\u77e9\u9635\uff0c\u4ee5\u53ca\u8be5\u7c7b\u578b\u5bf9\u5e94\u7684\u8fc7\u6ee4\u77e9\u9635\u3002\u5148\u6284\u4e09\u4e2a\u91cf\uff0c\u6bd4\u5bf9\u6709\u591a\u5c11\u8bfb\u6bb5\u80fd\u843d\u5230\u53c2\u8003\u57fa\u56e0\u7ec4\u3001\u539f\u59cb\u77e9\u9635\u91cc\u6709\u591a\u5c11\u975e\u96f6\u5206\u5b50\u8ba1\u6570\u3001\u8fc7\u6ee4\u540e\u7559\u4e0b\u591a\u5c11\u6761\u5f62\u7801\u3002\u8fd9\u4e09\u8005\u7684\u5206\u6bcd\u4e0d\u540c\u3002\u4e00\u4e2a\u6837\u672c\u7684\u6bd4\u5bf9\u7387\u5f88\u9ad8\uff0c\u53ea\u80fd\u8bf4\u660e\u5e8f\u5217\u4e0e\u53c2\u8003\u6709\u8f83\u597d\u5339\u914d\uff0c\u5e76\u4e0d\u4fdd\u8bc1\u5b83\u5e26\u7740\u53ef\u7528\u7684\u7ec6\u80de\u6761\u5f62\u7801\uff0c\u4e5f\u4e0d\u4fdd\u8bc1\u6700\u7ec8\u80fd\u5f52\u5165\u67d0\u4e2a\u57fa\u56e0\u3002<\/p>\n
\n\n\n\n\n\n\n\n
\u770b\u5230\u7684\u7ec4\u5408<\/th>\n\u5148\u590d\u6838\u4ec0\u4e48<\/th>\n\u6700\u6709\u5e2e\u52a9\u7684\u5bf9\u7167<\/th>\n<\/tr>\n<\/thead>\n
\u8f6c\u5f55\u672c\u7aef\u6bd4\u5bf9\u5f88\u5dee<\/td>\n\u8bfb\u6bb5\u7aef\u6b21\u5e8f\u3001\u7269\u79cd\u53ca\u53c2\u8003\u7d22\u5f15<\/td>\n\u540c\u4e00\u6279\u6210\u5bf9\u8bb0\u5f55\u7684\u8bfb\u957f\u548c\u6bd4\u5bf9\u65e5\u5fd7<\/td>\n<\/tr>\n
\u6bd4\u5bf9\u5c1a\u53ef\uff0c\u539f\u59cb\u77e9\u9635\u5f88\u7a00<\/td>\n\u6761\u5f62\u7801\u4f4d\u7f6e\u3001\u5141\u8bb8\u5217\u8868\u3001\u57fa\u56e0\u6ce8\u91ca\u4e0e\u8ba1\u6570\u5b9a\u4e49<\/td>\n\u5c0f\u6279\u6761\u5f62\u7801\u7cbe\u786e\u5339\u914d\u548c\u539f\u59cb\u5217\u7684\u5206\u5b50\u6570\u5206\u5e03<\/td>\n<\/tr>\n
\u539f\u59cb\u77e9\u9635\u6709\u4e00\u6279\u660e\u663e\u9ad8\u8ba1\u6570\u5217\uff0c\u8fc7\u6ee4\u540e\u5f88\u5c11<\/td>\n\u7ec6\u80de\u5224\u5b9a\u53ca\u80cc\u666f\u5206\u5e03<\/td>\n\u56fa\u5b9a\u540c\u4e00\u539f\u59cb\u77e9\u9635\uff0c\u5355\u72ec\u66f4\u6362\u8fc7\u6ee4\u8bbe\u7f6e<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u7ec6\u80de\u6570\u504f\u5c11\uff0c\u5148\u67e5\u54ea\u4e00\u6b65\uff1f\u3002\u5e38\u89c1
\u5e38\u89c1 10x 3\u2032 \u53cc\u7aef\u6587\u5e93\uff5c\u628a\u6545\u969c\u5b9a\u4f4d\u5728\u8fc7\u6ee4\u4e4b\u524d\u6216\u4e4b\u540e\u3002<\/figcaption><\/figure>\n
\u82e5\u95ee\u9898\u843d\u5728\u7b2c\u4e8c\u5c42\uff0c\u522b\u9057\u6f0f\u201c\u7ed9\u4ec0\u4e48\u7279\u5f81\u8ba1\u6570\u201d\u8fd9\u4e2a\u95ee\u9898\u3002\u53ea\u7edf\u8ba1\u5916\u663e\u5b50\u8bc1\u636e\uff0c\u4e0e\u5141\u8bb8\u8ba1\u5165\u57fa\u56e0\u8303\u56f4\u5185\u542b\u5b50\u8bc1\u636e\uff0c\u56de\u7b54\u7684\u662f\u4e0d\u540c\u95ee\u9898\u3002\u5355\u6838\u6d4b\u5e8f\u4e2d\u82e5\u672a\u526a\u63a5\u8f6c\u5f55\u672c\u8f83\u591a\uff0c\u8fd9\u4e2a\u5dee\u522b\u5c24\u5176\u503c\u5f97\u5148\u67e5\u3002\u8bb0\u5f55\u8fd0\u884c\u65f6\u7684 --soloFeatures<\/code>\u3001\u9009\u6765\u4e0b\u6e38\u5206\u6790\u7684\u5b50\u76ee\u5f55\uff0c\u4ee5\u53ca\u5efa\u7d22\u5f15\u6240\u7528\u7684\u6ce8\u91ca\u7248\u672c\u3002\u628a\u4e00\u5957\u53c2\u8003\u7684\u57fa\u56e0\u77e9\u9635\u548c\u53e6\u4e00\u5957\u53c2\u8003\u7684\u5168\u57fa\u56e0\u8303\u56f4\u77e9\u9635\u4ea4\u53c9\u6bd4\u8f83\uff0c\u4f1a\u628a\u8ba1\u6570\u5b9a\u4e49\u7684\u5dee\u5f02\u8bef\u8ba4\u4e3a\u662f\u7ec6\u80de\u5224\u5b9a\u9020\u6210\u7684\u635f\u5931\u3002STAR \u4e0e STARsolo \u7684\u53c2\u6570\u5b9a\u4e49<\/a><\/p>\n
\u5982\u679c\u6709\u4e24\u4efd\u5316\u5b66\u4f53\u7cfb\u76f8\u540c\u3001\u6765\u81ea\u540c\u4e00\u6279\u6b21\u7684\u6587\u5e93\uff0c\u53ef\u4ee5\u628a\u4e24\u4efd\u65e5\u5fd7\u7684\u540c\u4e00\u9879\u6307\u6807\u6a2a\u5411\u5bf9\u9f50\u3002\u53ea\u6709\u5176\u4e2d\u4e00\u4efd\u6761\u5f62\u7801\u8bc6\u522b\u5dee\uff0c\u4f18\u5148\u56de\u770b\u90a3\u4efd\u6587\u5e93\u7684\u6587\u4ef6\u4e0e\u6cf3\u9053\u62c6\u5206\u3002\u4e24\u4efd\u90fd\u5728\u540c\u4e00\u5904\u6389\u4e0b\u53bb\uff0c\u518d\u67e5\u5171\u6709\u7684\u767d\u540d\u5355\u3001\u53c2\u8003\u4e0e\u53c2\u6570\u3002\u8fd9\u79cd\u5bf9\u7167\u4e0d\u80fd\u6392\u9664\u5b9e\u9a8c\u95ee\u9898\uff0c\u4f46\u80fd\u7f29\u5c0f\u9700\u8981\u52a8\u624b\u91cd\u8dd1\u7684\u8303\u56f4\u3002<\/p>\n
\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u6545\u610f\u5bf9\u8c03\u4e24\u4efd FASTQ\uff0c\u770b\u9519\u8bef\u6700\u5148\u51fa\u73b0\u5728\u54ea\u91cc<\/h2>\n
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STARsolo \u63d0\u4f9b\u5728\u5df2\u6709\u539f\u59cb\u8ba1\u6570\u77e9\u9635\u4e0a\u5355\u72ec\u8fd0\u884c\u7ec6\u80de\u8fc7\u6ee4\u7684\u6a21\u5f0f\u3002\u628a\u539f\u59cb\u76ee\u5f55\u8bbe\u4e3a\u8f93\u5165\uff0c\u6307\u5b9a\u65b0\u7684\u8f93\u51fa\u524d\u7f00\uff0c\u5c31\u80fd\u4fdd\u7559\u6700\u521d\u7ed3\u8bba\uff0c\u6bd4\u8f83\u53e6\u4e00\u79cd\u7ec6\u80de\u5224\u5b9a\u5f97\u5230\u7684\u7ed3\u679c\u3002\u5b98\u65b9\u5217\u51fa\u7684 EmptyDrops_CR<\/code> \u5229\u7528\u4e0e\u73af\u5883\u80cc\u666f\u7684\u8868\u8fbe\u5dee\u5f02\u8bc6\u522b\u90e8\u5206\u4f4e\u8ba1\u6570\u6761\u5f62\u7801\u3002\u5b83\u548c\u7b80\u5355\u5730\u622a\u53d6\u5206\u5b50\u6570\u6700\u9ad8\u7684\u82e5\u5e72\u5217\u5177\u6709\u4e0d\u540c\u542b\u4e49\uff0c\u6838\u67e5\u7ed3\u679c\u65f6\u5e94\u5217\u51fa\u65b0\u589e\u548c\u79fb\u9664\u7684\u6761\u5f62\u7801\uff0c\u800c\u4e0d\u4ec5\u4ec5\u62a5\u4e24\u4e2a\u603b\u6570\u3002<\/p>\n
STAR --runMode soloCellFiltering Solo.out\/Gene\/raw\/ check_ \\\r\n  --soloCellFilter EmptyDrops_CR\r\n<\/code><\/pre>\n
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