{"id":67392,"date":"2026-11-14T20:02:32","date_gmt":"2026-11-14T12:02:32","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=67392"},"modified":"2026-11-14T20:48:24","modified_gmt":"2026-11-14T12:48:24","slug":"scanpy-anndata-x-raw-counts-normalization-audit","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/scanpy-anndata-x-raw-counts-normalization-audit\/","title":{"rendered":"Scanpy \u91cc\u540c\u4e00\u4e2a\u57fa\u56e0\u4e3a\u4f55\u4e00\u4f1a\u513f\u662f 2\uff0c\u4e00\u4f1a\u513f\u662f 7.60\uff1f\u5148\u8ba4\u6e05 X\u3001raw \u548c layers"},"content":{"rendered":"
\n

\u540c\u4e8b\u4f20\u6765\u4e00\u4e2a\u5df2\u7ecf\u5206\u7fa4\u7684\u5355\u7ec6\u80de\u5bf9\u8c61\u3002\u4f60\u60f3\u67e5\u67d0\u4e2a\u7ec6\u80de\u7684\u4e00\u4e2a\u57fa\u56e0\u7a76\u7adf\u6d4b\u5230\u4e86\u51e0\u4e2a\u5206\u5b50\uff0c\u6253\u5f00\u4e3b\u77e9\u9635\uff0c\u770b\u5230\u7684\u662f\u5e26\u5c0f\u6570\u7684 7.60\u3002\u7ffb\u5230\u53e6\u4e00\u4e2a\u5c42\u53c8\u53d8\u6210 2\u3002\u518d\u753b\u4e00\u5f20\u57fa\u56e0\u8868\u8fbe\u56fe\uff0c\u989c\u8272\u5374\u597d\u50cf\u6765\u81ea\u7b2c\u4e09\u5957\u6570\u503c\u3002\u5355\u7ec6\u80de\u5bf9\u8c61\u53ef\u4ee5\u540c\u65f6\u4fdd\u5b58\u591a\u4e2a\u5904\u7406\u9636\u6bb5\u7684\u8868\u8fbe\u6570\u636e\uff0c\u8981\u56de\u7b54\u201c\u68c0\u6d4b\u5230\u51e0\u4e2a\u5206\u5b50\u201d\uff0c\u5f97\u5148\u67e5\u8fd9\u4e2a\u6570\u662f\u4ece\u54ea\u4e00\u5c42\u53d6\u7684\uff0c\u4ee5\u53ca\u4fdd\u5b58\u8fd9\u4e00\u5c42\u4e4b\u524d\u505a\u8fc7\u4ec0\u4e48\u3002<\/p>\n

\u8fd9\u91cc\u4ece\u4e00\u4e2a\u5df2\u7ecf\u901a\u8fc7\u57fa\u7840\u8d28\u63a7\u3001\u5c1a\u672a\u5f52\u4e00\u5316\u7684\u8ba1\u6570\u77e9\u9635\u5f00\u59cb\u3002\u8fd9\u4e2a\u524d\u63d0\u5f88\u91cd\u8981\u3002\u5982\u679c\u6536\u5230\u7684\u6587\u4ef6\u53ea\u6709\u6807\u51c6\u5316\u540e\u7684\u8868\u8fbe\u503c\uff0c\u628a\u90a3\u4e00\u5c42\u6539\u540d\u4e3a\u201c\u8ba1\u6570\u201d\u4e0d\u4f1a\u5e26\u56de\u539f\u59cb\u5206\u5b50\u6570\u3002\u5c24\u5176\u5728\u516c\u5f00\u6570\u636e\u5e93\u9879\u76ee\u4e2d\uff0c\u5e94\u5148\u9605\u8bfb\u4f5c\u8005\u7684\u5904\u7406\u8bf4\u660e\uff0c\u786e\u8ba4\u6587\u4ef6\u662f\u539f\u59cb\u8ba1\u6570\u3001\u6587\u5e93\u5f52\u4e00\u5316\u8868\u8fbe\u8fd8\u662f\u5df2\u6309\u57fa\u56e0\u4e2d\u5fc3\u5316\u7684\u503c\u3002\u5355\u9760\u6587\u4ef6\u540e\u7f00\u8bc6\u522b\u4e0d\u4e86\u8fd9\u4e9b\u5dee\u522b\u3002<\/p>\n

\u5148\u628a\u4e09\u4e2a\u4f4d\u7f6e\u7684\u542b\u4e49\u5f04\u660e\u767d<\/h2>\n

adata.X<\/code> \u662f\u5f53\u524d\u4e3b\u77e9\u9635\u3002\u5f88\u591a\u9884\u5904\u7406\u51fd\u6570\u9ed8\u8ba4\u4fee\u6539\u5b83\uff0c\u56e0\u6b64\u5b83\u7684\u542b\u4e49\u4f1a\u968f\u64cd\u4f5c\u6539\u53d8\u3002adata.layers<\/code> \u662f\u53ef\u4ee5\u547d\u540d\u7684\u5e73\u884c\u77e9\u9635\u96c6\u5408\uff0c\u4f8b\u5982\u628a\u4e00\u4efd\u539f\u59cb\u5206\u5b50\u8ba1\u6570\u4fdd\u5b58\u5728\u540d\u4e3a counts<\/code> \u7684\u5c42\u4e2d\u3002\u4e00\u4e2a\u666e\u901a\u5c42\u5728\u7ec6\u80de\u4e0e\u57fa\u56e0\u4e24\u4e2a\u7ef4\u5ea6\u4e0a\u5e94\u4e0e\u5f53\u524d\u4e3b\u77e9\u9635\u5339\u914d\u3002\u540d\u5b57\u7531\u521b\u5efa\u5bf9\u8c61\u7684\u4eba\u51b3\u5b9a\uff0c\u53eb counts<\/code> \u5e76\u4e0d\u81ea\u52a8\u8bc1\u660e\u5176\u4e2d\u662f\u6574\u6570\u5206\u5b50\u8ba1\u6570\u3002AnnData \u7684 layers \u5b9a\u4e49<\/a><\/p>\n

adata.raw<\/code> \u5bb9\u6613\u5f15\u8d77\u53e6\u4e00\u79cd\u8bef\u4f1a\u3002\u5b83\u4fdd\u5b58\u7684\u662f\u4f60\u8bbe\u7f6e\u8fd9\u4e2a\u5feb\u7167\u65f6\u7684\u8868\u8fbe\u77e9\u9635\u548c\u7279\u5f81\u4fe1\u606f\u3002\u82e5\u5728\u5f52\u4e00\u5316\u548c\u53d6\u5bf9\u6570\u4e4b\u540e\u624d\u4fdd\u5b58\uff0c\u8bfb\u51fa\u6765\u7684\u5c31\u662f\u90a3\u4e2a\u9636\u6bb5\u7684\u503c\u3002\u8fd8\u6709\u4e00\u4e2a\u5f88\u5b9e\u7528\u7684\u5dee\u5f02\uff0c\u5bf9\u539f\u5bf9\u8c61\u6309\u7ec6\u80de\u5207\u7247\u65f6\uff0c\u5feb\u7167\u4e2d\u7684\u7ec6\u80de\u4e5f\u4f1a\u968f\u4e4b\u5207\u7247\u3002\u5bf9\u5f53\u524d\u77e9\u9635\u6309\u57fa\u56e0\u5207\u7247\u65f6\uff0c\u5148\u524d\u5feb\u7167\u91cc\u4fdd\u7559\u7684\u5176\u4ed6\u57fa\u56e0\u4ecd\u53ef\u80fd\u53ef\u4ee5\u8bfb\u53d6\u3002\u56e0\u6b64\uff0c\u4ece\u4e3b\u77e9\u9635\u91cc\u627e\u4e0d\u5230\u67d0\u4e2a\u6807\u8bb0\uff0c\u5148\u68c0\u67e5\u57fa\u56e0\u662f\u5426\u5728\u5feb\u7167\u4ee5\u53ca\u6700\u521d\u7684\u53c2\u8003\u6ce8\u91ca\u4e2d\uff0c\u518d\u5224\u65ad\u662f\u57fa\u56e0\u547d\u540d\u4e0d\u5339\u914d\u8fd8\u662f\u786e\u5b9e\u88ab\u8fc7\u6ee4\u3002AnnData \u5bf9 raw \u5feb\u7167\u4e0e\u5207\u7247\u7684\u8bf4\u660e<\/a><\/p>\n

\u7528\u4e24\u4e2a\u7ec6\u80de\u628a 7.60 \u7b97\u51fa\u6765<\/h2>\n

\u5047\u8bbe\u7532\u7ec6\u80de\u603b\u5171\u8ba1\u5230\u5341\u4e2a\u5206\u5b50\uff0c\u76ee\u6807\u57fa\u56e0\u5360\u4e24\u4e2a\u3002\u4e59\u7ec6\u80de\u603b\u5171\u8ba1\u5230\u4e8c\u5341\u4e2a\u5206\u5b50\uff0c\u76ee\u6807\u57fa\u56e0\u4e5f\u5360\u4e24\u4e2a\u3002\u628a\u6bcf\u4e2a\u7ec6\u80de\u7684\u603b\u91cf\u5f52\u4e00\u5230\u4e00\u4e07\uff0c\u7532\u7684\u76ee\u6807\u57fa\u56e0\u53d8\u4e3a\u4e24\u5343\uff0c\u4e59\u7684\u53d8\u4e3a\u4e00\u5343\u3002\u63a5\u7740\u53d6\u81ea\u7136\u5bf9\u6570\u5f62\u5f0f\u7684 log1p<\/code>\uff0c\u5f97\u5230\u7ea6 7.60 \u548c 6.91\u3002\u540c\u4e00\u4e2a\u539f\u59cb\u8ba1\u6570\u4e4b\u6240\u4ee5\u663e\u793a\u51fa\u4e0d\u540c\u989c\u8272\uff0c\u539f\u56e0\u5c31\u85cf\u5728\u4e24\u4e2a\u7ec6\u80de\u4e0d\u540c\u7684\u5206\u6bcd\u91cc\u3002\u53cd\u8fc7\u6765\uff0c\u8f83\u9ad8\u7684\u5bf9\u6570\u8868\u8fbe\u503c\u4e5f\u4e0d\u80fd\u5355\u72ec\u8bf4\u660e\u5b83\u6d4b\u5230\u4e86\u66f4\u591a\u76ee\u6807\u5206\u5b50\u3002<\/p>\n

\n\n\n\n\n\n\n
\u6559\u5b66\u7ec6\u80de<\/th>\n\u539f\u59cb\u8ba1\u6570 \/ \u5168\u7ec6\u80de\u603b\u91cf<\/th>\n\u5f52\u4e00\u5316\u81f3\u4e00\u4e07<\/th>\n\u518d\u53d6 log1p<\/th>\n<\/tr>\n<\/thead>\n
\u7532<\/td>\n2 \/ 10<\/td>\n2,000<\/td>\n7.60<\/td>\n<\/tr>\n
\u4e59<\/td>\n2 \/ 20<\/td>\n1,000<\/td>\n6.91<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u4e24\u4e2a\u5206\u5b50\uff0c\u600e\u4e48\u4f1a\u53d8\u6210
\u6559\u5b66\u7b97\u4f8b\uff5c\u4e24\u4e2a\u7ec6\u80de\u7684\u603b\u5206\u5b50\u6570\u5206\u522b\u4e3a 10 \u548c 20\u3002<\/figcaption><\/figure>\n

\u7b97\u5b8c\u8fd9\u4e24\u884c\u518d\u770b\u771f\u5b9e\u5bf9\u8c61\uff0c\u8bb0\u4f4f\u8fd9\u91cc\u660e\u786e\u6307\u5b9a\u4e86\u76ee\u6807\u603b\u91cf\uff0c\u5e76\u5047\u5b9a\u7528\u6240\u6709\u7279\u5f81\u8ba1\u7b97\u5f52\u4e00\u5316\u56e0\u5b50\u3002\u82e5\u5728\u5f52\u4e00\u5316\u524d\u5148\u5220\u9664\u5927\u91cf\u57fa\u56e0\uff0c\u6216\u542f\u7528\u6392\u9664\u9ad8\u8868\u8fbe\u57fa\u56e0\u7684\u9009\u9879\uff0c\u7528\u4f5c\u5206\u6bcd\u7684\u603b\u91cf\u53ef\u80fd\u6539\u53d8\u3002\u540c\u4e00\u4efd\u6570\u636e\u5728\u4e24\u4e2a\u9879\u76ee\u91cc\u51fa\u73b0\u4e0d\u540c\u6570\u503c\uff0c\u5148\u786e\u8ba4\u8fd9\u4e9b\u7ec6\u8282\u6bd4\u5148\u8c03\u989c\u8272\u4e0a\u9650\u6709\u6548\u3002\u67d0\u4e2a\u7ec6\u80de\u5982\u679c\u672c\u6765\u5c31\u662f\u7a7a\u5217\uff0c\u4e5f\u6ca1\u6709\u503c\u5f97\u6bd4\u8f83\u7684\u8868\u8fbe\u7ec4\u6210\uff0c\u5e94\u8be5\u56de\u5230\u8d28\u63a7\u73af\u8282\u8bf4\u660e\u5176\u53bb\u7559\u3002Scanpy \u5bf9\u603b\u91cf\u5f52\u4e00\u5316\u548c\u53ef\u9009\u53c2\u6570\u7684\u8bf4\u660e<\/a><\/p>\n

\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u5199\u5165\u78c1\u76d8\u518d\u8bfb\u56de\uff0c\u770b\u770b\u76ee\u6807\u57fa\u56e0\u7a76\u7adf\u8fd8\u5728\u54ea\u4e00\u5c42<\/h2>\n

\u5728 Python 3.12.14 \u4e2d\u5b89\u88c5 Scanpy 1.12.6 \u548c AnnData 0.13.4\u3002\u7528\u4e24\u679a\u7ec6\u80de\u3001\u6bcf\u679a\u4e24\u4e2a\u57fa\u56e0\u7684\u6574\u6570\u7a00\u758f\u77e9\u9635\u5b9e\u9645\u8fd0\u884c\u603b\u91cf\u5f52\u4e00\u5316\u548c\u53d6\u5bf9\u6570\uff0c\u76ee\u6807\u57fa\u56e0\u8f93\u51fa\u4e3a 7.601402 \u4e0e 6.908755\uff0c\u4fdd\u5b58\u7684\u8ba1\u6570\u5c42\u4ecd\u5206\u522b\u4e3a 2 \u548c 2\u3002\u968f\u540e\u628a\u5f52\u4e00\u5316\u540e\u7684\u5b8c\u6574\u5bf9\u8c61\u5b58\u5165 raw\uff0c\u4e3b\u5bf9\u8c61\u53ea\u7559\u4e0b\u80cc\u666f\u57fa\u56e0\uff0c\u5199\u6210 h5ad\uff0c\u518d\u4ece\u78c1\u76d8\u91cd\u65b0\u8bfb\u53d6\u3002\u6b64\u65f6\u4e3b\u77e9\u9635\u7684\u5f62\u72b6\u4e3a\u4e24\u884c\u4e00\u5217\uff0craw \u7684\u5f62\u72b6\u4ecd\u662f\u4e24\u884c\u4e24\u5217\uff0c\u4ece\u5feb\u7167\u91cc\u6309\u540d\u5b57\u53d6\u56de\u76ee\u6807\u57fa\u56e0\uff0c\u7ed3\u679c\u4e0e\u5207\u7247\u524d\u5b8c\u5168\u76f8\u540c\u3002<\/p>\n

\u8fd9\u4e2a\u5c0f\u5b9e\u9a8c\u80fd\u5e2e\u5fd9\u8bca\u65ad\u4e24\u79cd\u771f\u5b9e\u95ee\u9898\u3002\u82e5\u6309\u4e3b\u77e9\u9635\u627e\u4e0d\u5230\u57fa\u56e0\uff0c\u5148\u770b\u662f\u4e0d\u662f\u5728\u9ad8\u53d8\u57fa\u56e0\u7b5b\u9009\u65f6\u88ab\u79fb\u8d70\uff0c\u518d\u770b\u5feb\u7167\u5efa\u7acb\u5728\u7b5b\u9009\u4e4b\u524d\u8fd8\u662f\u4e4b\u540e\u3002\u82e5\u5feb\u7167\u91cc\u786e\u5b9e\u627e\u5230\u4e86\u8be5\u57fa\u56e0\uff0c\u8fd8\u8981\u8bfb\u53d6\u4e00\u679a\u7ec6\u80de\u7684\u5177\u4f53\u6570\u503c\uff0c\u786e\u8ba4\u5b83\u662f\u8ba1\u6570\u8fd8\u662f\u5bf9\u6570\u8868\u8fbe\u3002\u4e00\u6b21\u5199\u76d8\u3001\u8bfb\u56de\u3001\u6309\u540d\u5b57\u53d6\u6570\uff0c\u6bd4\u4ec5\u4ec5\u6253\u5370\u5bf9\u8c61\u4e2d\u662f\u5426\u5b58\u5728 raw \u5b57\u6bb5\u66f4\u80fd\u8bf4\u660e\u4ea4\u4ed8\u6587\u4ef6\u91cc\u4fdd\u7559\u4e86\u4ec0\u4e48\u3002<\/p>\n

\u7528\u53ef\u590d\u67e5\u7684\u987a\u5e8f\u4fdd\u5b58\u81ea\u5df1\u7684\u5bf9\u8c61<\/h2>\n

\u4e0b\u9762\u51e0\u884c\u53ea\u9002\u7528\u4e8e\u5df2\u786e\u8ba4\u4e3b\u77e9\u9635\u5b58\u653e\u539f\u59cb\u8ba1\u6570\u7684\u5bf9\u8c61\u3002\u5148\u590d\u5236\u4e00\u4efd\u539f\u59cb\u8ba1\u6570\uff0c\u518d\u5904\u7406\u4e3b\u77e9\u9635\uff0c\u6700\u540e\u628a\u5904\u7406\u540e\u7684\u5b8c\u6574\u57fa\u56e0\u8868\u8fbe\u8bbe\u4e3a\u5feb\u7167\u3002\u590d\u5236\u77e9\u9635\u662f\u4e3a\u4e86\u8ba9\u540e\u7eed\u6539\u52a8\u4e0d\u5f71\u54cd\u5df2\u7ecf\u4fdd\u5b58\u7684\u6570\u636e\u3002\u5982\u679c\u4f60\u9700\u8981\u4ec5\u4ee5\u9ad8\u53d8\u57fa\u56e0\u8ba1\u7b97\u964d\u7ef4\uff0c\u53ef\u4ee5\u5728\u8fd9\u4e00\u6b65\u4e4b\u540e\u518d\u5bf9\u5b50\u5bf9\u8c61\u7b5b\u9009\u57fa\u56e0\uff0c\u5e76\u5728\u6587\u4ef6\u8bf4\u660e\u91cc\u8bb0\u4e0b\u8fd9\u4e00\u9009\u62e9\u3002<\/p>\n

import scanpy as sc\r\n\r\nadata.layers["counts"] = adata.X.copy()\r\nsc.pp.normalize_total(adata, target_sum=1e4)\r\nsc.pp.log1p(adata)\r\nadata.raw = adata.copy()\r\nprint(list(adata.layers.keys()), adata.shape, adata.raw.shape)\r\n<\/code><\/pre>\n
\u62ff\u5230\u522b\u4eba\u505a\u597d\u7684\u5bf9\u8c61\uff0c\u4e0d\u59a8\u9009\u4e00\u4e2a\u7ec6\u80de\u3001\u4e24\u4e2a\u57fa\u56e0\uff0c\u6cbf\u7740\u5404\u5c42\u8ffd\u8e2a\u5750\u6807\u3002\u67e5\u770b obs_names<\/code> \u548c var_names<\/code> \u540e\u6309\u540d\u5b57\u53d6\u6570\uff0c\u907f\u514d\u76f4\u63a5\u5047\u8bbe\u4e24\u4e2a\u6587\u4ef6\u4e2d\u7684\u884c\u53f7\u76f8\u540c\u3002\u5148\u627e\u4e00\u4e2a\u6709\u660e\u663e\u975e\u96f6\u8868\u8fbe\u7684\u6807\u8bb0\uff0c\u518d\u627e\u4e00\u4e2a\u4f4e\u8868\u8fbe\u6807\u8bb0\u3002\u524d\u8005\u5e2e\u52a9\u6838\u5bf9\u53d8\u6362\u7684\u6570\u91cf\u7ea7\uff0c\u540e\u8005\u80fd\u53d1\u73b0\u663e\u793a\u65f6\u88ab\u9690\u53bb\u7684\u5f31\u4fe1\u53f7\u3002\u82e5\u4e3b\u77e9\u9635\u51fa\u73b0\u8d1f\u6570\uff0c\u4e00\u4e2a\u53ef\u80fd\u539f\u56e0\u662f\u540e\u6765\u8fdb\u884c\u4e86\u4e2d\u5fc3\u5316\u6216\u5176\u4ed6\u53d8\u6362\uff0c\u5e94\u8ffd\u67e5\u5206\u6790\u8bb0\u5f55\u3002\u4ec5\u51ed\u201c\u5168\u662f\u6574\u6570\u201d\u6216\u201c\u5b58\u5728\u5c0f\u6570\u201d\u90fd\u4e0d\u8db3\u4ee5\u6062\u590d\u5bf9\u8c61\u5386\u53f2\u3002<\/p>\n
\u8fd8\u53ef\u4ee5\u505a\u4e00\u9879\u5f88\u4fbf\u5b9c\u7684\u53cd\u67e5\u3002\u5728\u5f53\u521d\u7684\u5168\u57fa\u56e0\u8ba1\u6570\u77e9\u9635\u91cc\uff0c\u7528\u540c\u4e00\u4e2a\u7ec6\u80de\u8ba1\u7b97\u884c\u548c\uff0c\u518d\u7528\u76ee\u6807\u57fa\u56e0\u7684\u6574\u6570\u8ba1\u6570\u9664\u4ee5\u8fd9\u4e2a\u884c\u548c\uff0c\u6309\u5904\u7406\u8bf4\u660e\u91cd\u7b97\u5bf9\u6570\u503c\u3002\u5982\u679c\u591a\u6570\u57fa\u56e0\u90fd\u5bf9\u5f97\u4e0a\u800c\u5c11\u6570\u5750\u6807\u5bf9\u4e0d\u4e0a\uff0c\u5148\u6838\u5bf9\u7279\u5f81\u7f16\u53f7\u548c\u91cd\u590d\u57fa\u56e0\u540d\u3002\u5982\u679c\u540c\u4e00\u7ec6\u80de\u7684\u8bb8\u591a\u503c\u540c\u65f6\u504f\u79fb\uff0c\u5219\u66f4\u50cf\u662f\u4f7f\u7528\u4e86\u4e0d\u540c\u7684\u5f52\u4e00\u5316\u5206\u6bcd\u3002\u8fd9\u4e2a\u987a\u5e8f\u80fd\u5e2e\u4f60\u51b3\u5b9a\u5148\u627e\u6ce8\u91ca\u6587\u4ef6\u8fd8\u662f\u5148\u7ffb\u9884\u5904\u7406\u811a\u672c\u3002<\/p>\n
\u753b\u6807\u8bb0\u56fe\u548c\u505a\u68c0\u9a8c\u65f6\uff0c\u4e3b\u52a8\u6307\u5b9a\u6765\u6e90<\/h2>\n
\u5982\u679c\u753b\u56fe\u7684\u4efb\u52a1\u662f\u663e\u793a\u5f52\u4e00\u5316\u540e\u7684\u6807\u8bb0\u8868\u8fbe\uff0c\u5e94\u5728\u65b9\u6cd5\u8bf4\u660e\u4e2d\u5199\u6e05\u5b83\u6765\u81ea\u5feb\u7167\u3001\u4e3b\u77e9\u9635\u8fd8\u662f\u67d0\u4e2a\u6307\u5b9a\u5c42\u3002\u9047\u5230\u4e24\u4e2a\u56fe\u4e0d\u4e00\u81f4\uff0c\u53ef\u4ee5\u5148\u5f3a\u5236\u4f7f\u7528\u540c\u4e00\u4e2a\u6570\u636e\u6765\u6e90\uff0c\u518d\u6bd4\u8f83\u5206\u7ec4\u3001\u8272\u9636\u4ee5\u53ca\u7b5b\u6389\u7684\u7ec6\u80de\u3002\u4e00\u4e2a\u5178\u578b\u9677\u9631\u662f\u5148\u5728\u4e3b\u77e9\u9635\u4e0a\u505a\u4e86\u57fa\u56e0\u7f29\u653e\uff0c\u5374\u8ba9\u7ed8\u56fe\u51fd\u6570\u81ea\u52a8\u9009\u62e9\u4ecd\u7136\u5b58\u5728\u7684\u65e7\u5feb\u7167\u3002\u89c6\u89c9\u5dee\u5f02\u6709\u65f6\u662f\u56e0\u4e3a\u6bd4\u8f83\u5bf9\u8c61\u4e0d\u540c\uff0c\u4e0d\u80fd\u53ea\u9760\u8c03\u8272\u89e3\u51b3\u3002<\/p>\n
\u505a\u6807\u8bb0\u57fa\u56e0\u6392\u5e8f\u4e5f\u6709\u5177\u4f53\u7684\u8f93\u5165\u7ea6\u5b9a\u3002Scanpy \u7684 rank_genes_groups<\/code> \u6587\u6863\u8981\u6c42\u4f7f\u7528\u5bf9\u6570\u5316\u6570\u636e\uff0c\u5e76\u8bf4\u660e use_raw<\/code> \u548c layer<\/code> \u5982\u4f55\u9009\u62e9\u3002\u5c06\u539f\u59cb\u6574\u6570\u8ba1\u6570\u5c42\u76f4\u63a5\u4f20\u7ed9\u4e00\u4e2a\u9884\u671f\u5bf9\u6570\u8868\u8fbe\u7684\u6d41\u7a0b\uff0c\u8f93\u51fa\u8868\u683c\u53ef\u80fd\u7167\u6837\u751f\u6210\uff0c\u4f46\u5176\u6392\u5e8f\u548c\u6548\u5e94\u91cf\u89e3\u91ca\u4f1a\u504f\u79bb\u9884\u671f\u3002\u82e5\u5b9e\u9645\u8981\u6bd4\u8f83\u72ec\u7acb\u4f9b\u4f53\u7684\u5904\u7406\u6548\u5e94\uff0c\u5e94\u5355\u72ec\u8bbe\u8ba1\u6309\u751f\u7269\u5b66\u6837\u672c\u6c47\u603b\u7684\u6bd4\u8f83\uff0c\u4fdd\u7559\u539f\u59cb\u8ba1\u6570\u548c\u4f9b\u4f53\u6807\u7b7e\u3002\u4e0d\u8981\u628a\u5206\u7fa4\u540e\u7684\u7ec6\u80de\u5f53\u6210\u72ec\u7acb\u4e2a\u4f53\u6765\u589e\u52a0\u5b9e\u9a8c\u91cd\u590d\u6570\u3002Scanpy \u6807\u8bb0\u57fa\u56e0\u6392\u5e8f\u7684\u8f93\u5165\u8bf4\u660e<\/a><\/p>\n
\u4ea4\u4ed8\u5bf9\u8c61\u65f6\uff0c\u7ed9\u6bcf\u4e2a\u8868\u8fbe\u5c42\u5199\u4e00\u53e5\u6765\u5386\uff0c\u518d\u9644\u4e0a\u4e00\u4e2a\u7ec6\u80de\u4e0e\u4e00\u4e2a\u57fa\u56e0\u5728\u5404\u5c42\u7684\u5b9e\u9645\u8bfb\u6570\u3002\u63a5\u624b\u4eba\u80fd\u7528\u8fd9\u6761\u8bb0\u5f55\u9a8c\u8bc1\u6587\u4ef6\u662f\u5426\u8bfb\u5bf9\uff0c\u4e5f\u80fd\u77e5\u9053\u54ea\u4e9b\u503c\u9002\u5408\u6c42\u548c\u3001\u54ea\u4e9b\u503c\u9002\u5408\u753b\u56fe\u3002\u82e5\u56e2\u961f\u8981\u8ba9\u540c\u4e00\u4efd\u56fe\u8c31\u548c\u5904\u7406\u811a\u672c\u957f\u671f\u7531\u591a\u4eba\u590d\u67e5\uff0c\u53ef\u4ee5\u968f\u5bf9\u8c61\u4fdd\u5b58\u4f9d\u8d56\u7248\u672c\u3002\u9700\u8981\u516c\u5171\u5206\u6790\u73af\u5883\u65f6\uff0c\u53ef\u4e86\u89e3\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668<\/a>\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u4ece\u539f\u59cb\u6574\u6570\u8ba1\u6570\u7b97\u5230\u5bf9\u6570\u8868\u8fbe\uff0c\u8bf4\u660e Scanpy \u5bf9\u8c61\u7684 X\u3001raw \u548c layers \u5404\u81ea\u4fdd\u5b58\u4ec0\u4e48\uff0c\u5e76\u6f14\u793a\u5982\u4f55\u8bb0\u5f55\u6570\u636e\u6765\u6e90\u3002<\/p>\n","protected":false},"author":1,"featured_media":67391,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[51,71],"tags":[508,240,641],"class_list":["post-67392","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-python","category-single-cell","tag-anndata","tag-scanpy","tag-641"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/scanpy-anndata-x-raw-counts-normalization-audit-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/67392","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=67392"}],"version-history":[{"count":2,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/67392\/revisions"}],"predecessor-version":[{"id":68505,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/67392\/revisions\/68505"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/67391"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=67392"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=67392"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=67392"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}