{"id":68076,"date":"2026-11-14T20:11:48","date_gmt":"2026-11-14T12:11:48","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=68076"},"modified":"2026-11-14T20:11:48","modified_gmt":"2026-11-14T12:11:48","slug":"monocle3-root-partition-infinite-pseudotime","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/monocle3-root-partition-infinite-pseudotime\/","title":{"rendered":"Monocle 3 \u7684\u62df\u65f6\u5e8f\u4e3a\u4ec0\u4e48\u4f1a\u53cd\u8fc7\u6765\uff1f\u4ece\u6839\u7ec6\u80de\u548c\u65e0\u7a77\u503c\u67e5\u8d77"},"content":{"rendered":"
\n

\u62ff\u5230\u4e00\u5f20\u5206\u652f\u56fe\uff0c\u5b66\u751f\u8bf4\u989c\u8272\u4ece\u5de6\u5230\u53f3\u8d8a\u6765\u8d8a\u6df1\uff0c\u8bf4\u660e\u53f3\u4fa7\u7ec6\u80de\u53d1\u80b2\u5f97\u66f4\u665a\u3002\u5bfc\u5e08\u628a\u56fe\u65cb\u8f6c\u534a\u5708\uff0c\u540c\u6837\u4e00\u6279\u7ec6\u80de\u53c8\u6210\u4e86\u201c\u4ece\u53f3\u5230\u5de6\u201d\u3002\u771f\u6b63\u7ed9\u62df\u65f6\u5e8f\u8d4b\u4e88\u65b9\u5411\u7684\u662f\u7814\u7a76\u8005\u9009\u62e9\u7684\u8d77\u70b9\u548c\u6a21\u578b\u5b66\u4e60\u5230\u7684\u56fe\uff0c\u800c\u4e0d\u662f\u4e8c\u7ef4\u5750\u6807\u7684\u6a2a\u8f74\u3002\u4f7f\u7528 Monocle 3 \u65f6\uff0c\u628a\u6839\u4ece\u54ea\u513f\u6765\u3001\u54ea\u4e9b\u7ec6\u80de\u80fd\u4ece\u6839\u5230\u8fbe\u8bf4\u6e05\u695a\uff0c\u5f80\u5f80\u6bd4\u6362\u4e00\u4e2a\u66f4\u597d\u770b\u7684\u914d\u8272\u66f4\u91cd\u8981\u3002<\/p>\n

\u5148\u628a\u95ee\u9898\u9650\u5b9a\u4e3a\u540c\u4e00\u5019\u9009\u8c31\u7cfb\u4e2d\u7684\u72b6\u6001\u53d8\u5316\uff0c\u4f8b\u5982\u5728\u660e\u786e\u65f6\u95f4\u70b9\u53d6\u6837\u7684\u5206\u5316\u7cfb\u7edf\u4e2d\u6574\u7406\u65e9\u671f\u5230\u665a\u671f\u7684\u7ec6\u80de\u3002\u82e5\u628a\u6ca1\u6709\u5171\u540c\u8c31\u7cfb\u4f9d\u636e\u7684\u591a\u79cd\u6210\u719f\u7ec6\u80de\u653e\u5728\u4e00\u8d77\uff0c\u7a0b\u5e8f\u540c\u6837\u53ef\u4ee5\u7ed9\u51fa\u4e00\u5f20\u56fe\uff0c\u4f46\u8fd9\u5f20\u56fe\u5f88\u96be\u5355\u9760\u8868\u8fbe\u76f8\u4f3c\u6027\u8bc1\u660e\u4e00\u79cd\u7ec6\u80de\u771f\u7684\u53d8\u6210\u53e6\u4e00\u79cd\u3002\u5b9e\u9a8c\u65f6\u95f4\u3001\u8c31\u7cfb\u6807\u8bb0\u3001\u8ffd\u8e2a\u5b9e\u9a8c\u548c\u5df2\u77e5\u7684\u53d1\u80b2\u987a\u5e8f\uff0c\u90fd\u53ef\u4ee5\u7528\u6765\u652f\u6301\u6216\u8d28\u7591\u8d77\u70b9\u3002<\/p>\n

\u533a\u5206\u8868\u8fbe\u56fe\u3001\u4e3b\u56fe\u548c\u6839\u7ec6\u80de<\/h2>\n

Monocle 3 \u7684\u5e38\u7528\u987a\u5e8f\u662f\u9884\u5904\u7406\u3001\u964d\u7ef4\u3001\u805a\u7c7b\uff0c\u968f\u540e\u5b66\u4e60\u8fde\u63a5\u72b6\u6001\u7684\u4e3b\u56fe\uff0c\u518d\u6307\u5b9a\u8d77\u70b9\u7ed9\u7ec6\u80de\u6392\u5e8f\u3002\u964d\u7ef4\u56fe\u4e0a\u4e24\u4e2a\u70b9\u9760\u8fd1\uff0c\u8868\u793a\u5728\u6784\u9020\u8fd9\u5f20\u56fe\u6240\u7528\u7684\u8868\u793a\u4e2d\u8f83\u76f8\u4f3c\u3002\u4e3b\u56fe\u5219\u662f\u5728\u8be5\u7ed3\u6784\u4e0a\u5b66\u4e60\u51fa\u6765\u7684\u8def\u5f84\u3002\u6307\u5b9a\u6839\u4e4b\u540e\uff0c\u62df\u65f6\u5e8f\u6309\u7ec6\u80de\u5728\u56fe\u4e0a\u76f8\u5bf9\u4e8e\u6839\u7684\u4f4d\u7f6e\u8ba1\u7b97\u3002\u5b83\u4e0d\u662f\u91c7\u6837\u540e\u7684\u949f\u8868\u65f6\u95f4\u3002\u9664\u975e\u6709\u5355\u72ec\u7684\u5916\u90e8\u6807\u5b9a\uff0c\u4e0d\u5e94\u5c06\u67d0\u4e24\u4e2a\u6570\u4e4b\u95f4\u7684\u5dee\u76f4\u63a5\u5199\u6210\u5206\u5316\u82e5\u5e72\u5c0f\u65f6\u3002Monocle 3 \u8f68\u8ff9\u5206\u6790\u6559\u7a0b<\/a><\/p>\n

\u6709\u4e86\u5b9e\u9a8c\u65f6\u95f4\u4e5f\u4e0d\u80fd\u628a\u6700\u65e9\u65f6\u95f4\u70b9\u7684\u6240\u6709\u7ec6\u80de\u81ea\u52a8\u89c6\u4e3a\u7956\u7ec6\u80de\u3002\u65e9\u671f\u6837\u672c\u53ef\u80fd\u6df7\u6709\u6210\u719f\u6b8b\u7559\u7ec6\u80de\uff0c\u540e\u671f\u6837\u672c\u4e5f\u53ef\u80fd\u4fdd\u7559\u5c11\u91cf\u8d77\u59cb\u7ec6\u80de\u3002\u53ef\u4ee5\u4ece\u6700\u65e9\u7684\u65f6\u95f4\u70b9\u5148\u786e\u5b9a\u5019\u9009\uff0c\u518d\u7528\u4e00\u6574\u7ec4\u6807\u8bb0\u3001\u7ec6\u80de\u8d28\u91cf\u53ca\u6240\u5c5e\u5206\u533a\u8fdb\u4e00\u6b65\u7f29\u5c0f\u8303\u56f4\u3002\u5e94\u5c3d\u91cf\u8ba9\u6839\u7684\u9009\u62e9\u72ec\u7acb\u4e8e\u6700\u540e\u8981\u9a8c\u8bc1\u7684\u90a3\u6761\u5019\u9009\u57fa\u56e0\u66f2\u7ebf\uff0c\u907f\u514d\u6309\u7ed3\u679c\u6311\u6700\u987a\u773c\u7684\u8d77\u70b9\uff0c\u7136\u540e\u53c8\u62ff\u540c\u4e00\u66f2\u7ebf\u6765\u8bc1\u660e\u8d77\u70b9\u9009\u5f97\u5bf9\u3002<\/p>\n

\u4e94\u4e2a\u8282\u70b9\u63ed\u793a\u65b9\u5411\u4ece\u4f55\u800c\u6765<\/h2>\n

\u8bbe\u6709\u4e00\u4e2a\u6559\u5b66\u4e3b\u56fe\u3002\u7532\u8fde\u4e59\uff0c\u4e59\u8fde\u4e19\uff0c\u4e19\u5206\u522b\u8fde\u4e01\u548c\u620a\uff0c\u6bcf\u6761\u8fb9\u957f\u6682\u5b9a\u4e3a\u4e00\u3002\u5c06\u7532\u8bbe\u4e3a\u6839\uff0c\u4ece\u7532\u5230\u4e59\u662f\u4e00\u6761\u8fb9\uff0c\u5230\u4e19\u662f\u4e24\u6761\uff0c\u5230\u4e01\u548c\u620a\u90fd\u662f\u4e09\u6761\u3002\u518d\u4fdd\u6301\u540c\u4e00\u5f20\u56fe\uff0c\u4ec5\u628a\u6839\u6362\u5230\u4e01\u3002\u6b64\u65f6\u8ddd\u79bb\u4f9d\u6b21\u53d8\u6210\u7532\u4e09\u3001\u4e59\u4e8c\u3001\u4e19\u4e00\u3001\u4e01\u96f6\u3001\u620a\u4e8c\u3002\u4e01\u4e0e\u620a\u4ecd\u662f\u4e24\u652f\uff0c\u4f46\u5bf9\u5b83\u4eec\u8c01\u66f4\u201c\u665a\u201d\u7684\u89e3\u91ca\u5df2\u7ecf\u6539\u53d8\u3002\u771f\u5b9e\u56fe\u4e0a\u4f7f\u7528\u7684\u8ddd\u79bb\u548c\u7ec6\u80de\u6295\u5f71\u8fdc\u6bd4\u8fd9\u4e2a\u4f8b\u5b50\u590d\u6742\uff0c\u8fd9\u91cc\u53ea\u6f14\u793a\u4e3a\u4f55\u540c\u4e00\u5f62\u72b6\u53ef\u4ee5\u83b7\u5f97\u4e0d\u540c\u7684\u987a\u5e8f\u3002<\/p>\n

\n\n\n\n\n\n\n
\u4f5c\u4e3a\u8d77\u70b9<\/th>\n\u7532<\/th>\n\u4e59<\/th>\n\u4e19<\/th>\n\u4e01<\/th>\n\u620a<\/th>\n<\/tr>\n<\/thead>\n
\u7532<\/td>\n0<\/td>\n1<\/td>\n2<\/td>\n3<\/td>\n3<\/td>\n<\/tr>\n
\u4e01<\/td>\n3<\/td>\n2<\/td>\n1<\/td>\n0<\/td>\n2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u540c\u4e00\u5f20\u4e3b\u56fe\uff0c\u6362\u6839\u4ee5\u540e\u4f1a\u600e\u6837\u3002\u6559\u5b66\u56fe\uff5c\u7532\u8fde\u4e59\uff0c\u4e59\u8fde\u4e19\uff0c\u4e19\u5206\u522b\u8fde\u4e01\u548c\u620a\uff0c\u6bcf\u6761\u8fb9\u957f\u4e3a\u4e00\u3002\u6839\u5728\u7532\uff0c\u7532\u3001\u4e59\u3001\u4e19\u3001\u4e01\u3001\u620a\uff0c\u79bb\u6839\u8ddd\u79bb\u4f9d\u6b21\u4e3a
\u6559\u5b66\u56fe\uff5c\u7532\u8fde\u4e59\uff0c\u4e59\u8fde\u4e19\uff0c\u4e19\u5206\u522b\u8fde\u4e01\u548c\u620a\uff0c\u6bcf\u6761\u8fb9\u957f\u4e3a\u4e00\u3002<\/figcaption><\/figure>\n

\u8fd9\u5f20\u8868\u4e5f\u89e3\u91ca\u4e86\u4e00\u4e2a\u5e38\u89c1\u7684\u5199\u4f5c\u9519\u8bef\u3002\u4e01\u4e0e\u620a\u79bb\u7532\u8ddd\u79bb\u76f8\u540c\uff0c\u53ea\u80fd\u8bf4\u660e\u5728\u6b64\u56fe\u4e2d\u8ddd\u79bb\u76f8\u7b49\uff0c\u4e0d\u80fd\u63a8\u51fa\u4e24\u6761\u652f\u8def\u7ecf\u5386\u4e86\u76f8\u540c\u7684\u771f\u5b9e\u65f6\u95f4\u3002\u6cbf\u67d0\u4e00\u652f\u8d8a\u6765\u8d8a\u9ad8\u7684\u57fa\u56e0\uff0c\u4e5f\u53ef\u80fd\u6765\u81ea\u7ec6\u80de\u5468\u671f\u3001\u5e94\u6fc0\u6216\u6837\u672c\u51c6\u5907\uff0c\u800c\u975e\u8fd9\u6761\u652f\u8def\u7684\u8c03\u63a7\u56e0\u5b50\u3002\u5148\u628a\u8f68\u8ff9\u4f4d\u7f6e\u4e0e\u53d6\u6750\u65f6\u95f4\u3001\u4f9b\u4f53\u3001\u5df2\u77e5\u6807\u8bb0\u5206\u522b\u5e76\u6392\u753b\u51fa\uff0c\u8bfb\u8005\u624d\u80fd\u5224\u65ad\u8272\u5e26\u6240\u4ee3\u8868\u7684\u751f\u7269\u5b66\u89e3\u91ca\u662f\u5426\u7ad9\u5f97\u4f4f\u3002<\/p>\n

\u6709\u4e9b\u7ec6\u80de\u7684\u62df\u65f6\u5e8f\u4e3a\u4ec0\u4e48\u662f Inf<\/h2>\n

\u5728\u9ed8\u8ba4\u6309\u5206\u533a\u5b66\u4e60\u56fe\u7684\u60c5\u5f62\u4e0b\uff0c\u4e0d\u540c\u5206\u533a\u4e4b\u95f4\u53ef\u80fd\u6ca1\u6709\u53ef\u8d70\u7684\u8def\u5f84\u3002\u5982\u679c\u53ea\u5728\u7b2c\u4e00\u5757\u56fe\u91cc\u63d0\u4f9b\u6839\uff0c\u53e6\u4e00\u5757\u56fe\u91cc\u7684\u7ec6\u80de\u65e0\u6cd5\u4ece\u8fd9\u4e2a\u6839\u5230\u8fbe\uff0c\u62df\u65f6\u5e8f\u53ef\u80fd\u663e\u793a\u4e3a\u65e0\u7a77\u503c\u3002\u4e0d\u8981\u4e3a\u4e86\u8ba9\u7ed8\u56fe\u51fd\u6570\u63a5\u53d7\u8f93\u5165\uff0c\u5c31\u628a\u8fd9\u4e9b\u503c\u66ff\u6362\u6210\u5168\u4f53\u6700\u5927\u503c\u3002\u90a3\u6837\u4f1a\u628a\u201c\u6ca1\u6709\u8def\u5f84\u201d\u4f2a\u88c5\u6210\u201c\u65f6\u95f4\u6700\u665a\u201d\uff0c\u518d\u505a\u8d8b\u52bf\u62df\u5408\u5c31\u53ef\u80fd\u9020\u51fa\u4e00\u4e2a\u5b8c\u5168\u7531\u7f16\u7801\u65b9\u5f0f\u5236\u9020\u7684\u672b\u7aef\u7fa4\u3002<\/p>\n

\u4e0b\u9762\u5047\u5b9a cds<\/code> \u5df2\u5b8c\u6210\u964d\u7ef4\u3001\u805a\u7c7b\u548c\u4e3b\u56fe\u5b66\u4e60\uff0c\u5143\u6570\u636e\u4e2d\u5df2\u6709\u7814\u7a76\u8005\u6838\u5b9e\u8fc7\u7684\u65e9\u671f\u5019\u9009\u6807\u8bb0 root_ok<\/code>\u3002\u53ea\u5728\u6709\u751f\u7269\u5b66\u4f9d\u636e\u65f6\u628a\u8be5\u5217\u8bbe\u4e3a\u771f\uff0c\u518d\u7ed9\u7ec6\u80de\u6392\u5e8f\u3002\u4f8b\u5b50\u4e2d\u7684\u4ea4\u53c9\u8868\u6bd4\u4e00\u5f20\u5f69\u8272\u56fe\u66f4\u5bb9\u6613\u770b\u51fa\uff0c\u672a\u83b7\u5f97\u6709\u9650\u503c\u7684\u7ec6\u80de\u662f\u5426\u96c6\u4e2d\u5728\u67d0\u4e00\u5206\u533a\u3002order_cells \u7684\u53c2\u6570\u4e0e\u8fd4\u56de\u503c<\/a><\/p>\n

library(monocle3)\r\ncandidates <- colnames(cds)[which(SummarizedExperiment::colData(cds)$root_ok)]\r\nstopifnot(length(candidates) > 0)\r\ncds <- order_cells(cds, root_cells = candidates)\r\ntime <- pseudotime(cds)\r\ntable(partition = partitions(cds)[names(time)], finite = is.finite(time))\r\n<\/code><\/pre>\n
\u5982\u679c\u53e6\u4e00\u4e2a\u5206\u533a\u6709\u72ec\u7acb\u8bc1\u636e\u652f\u6301\u81ea\u5df1\u7684\u8d77\u70b9\uff0c\u53ef\u4ee5\u5728\u5176\u4e2d\u4e5f\u9009\u6839\uff0c\u7136\u540e\u5206\u533a\u89e3\u91ca\u3002\u6b64\u65f6\u4e0d\u540c\u5206\u533a\u7684\u96f6\u70b9\u6765\u81ea\u4e0d\u540c\u7ec6\u80de\u7fa4\uff0c\u6570\u8f74\u4e0d\u80fd\u81ea\u52a8\u5e76\u6210\u4e00\u6761\u5171\u540c\u7684\u771f\u5b9e\u65f6\u95f4\u7ebf\u3002\u5982\u679c\u7814\u7a76\u53ea\u9488\u5bf9\u7b2c\u4e00\u6761\u8c31\u7cfb\uff0c\u4e5f\u53ef\u628a\u7b2c\u4e8c\u4e2a\u5206\u533a\u5355\u72ec\u6807\u8bb0\u4e3a\u672c\u5206\u6790\u4e4b\u5916\uff0c\u5e76\u62a5\u544a\u539f\u56e0\u3002\u5bf9\u4e8e\u5177\u6709\u660e\u663e\u6279\u6b21\u9694\u79bb\u6216\u5305\u542b\u591a\u6761\u65e0\u5173\u8c31\u7cfb\u7684\u6570\u636e\uff0c\u5148\u67e5\u5206\u533a\u4ea7\u751f\u7684\u539f\u56e0\uff0c\u518d\u8c08\u662f\u5426\u5e94\u6784\u5efa\u4e00\u6761\u8de8\u533a\u8def\u5f84\u3002<\/p>\n
\u9009\u6839\u65f6\u8fd8\u6709\u4e00\u4e2a\u7ec6\u8282\u3002\u5982\u679c\u628a\u5206\u53c9\u540e\u7684\u4e24\u7aef\u7ec6\u80de\u90fd\u5217\u4e3a\u6839\uff0c\u4e3b\u56fe\u672c\u8eab\u4ecd\u53ef\u4fdd\u6301\u539f\u6837\uff0c\u4f46\u4ece\u6700\u8fd1\u8d77\u70b9\u8ba1\u8ddd\u79bb\u4f1a\u628a\u4e24\u7aef\u540c\u65f6\u5f53\u4f5c\u65e9\u671f\u533a\u57df\u3002\u53ef\u5728\u4e0a\u9762\u7684\u4e94\u8282\u70b9\u5c0f\u56fe\u4e0a\u624b\u7b97\uff0c\u5c06\u7532\u548c\u4e01\u90fd\u8bbe\u4e3a\u8d77\u70b9\uff0c\u4e59\u79bb\u7532\u4e00\u6b65\uff0c\u4e19\u79bb\u4e01\u4e00\u6b65\uff0c\u620a\u8ddd\u4e01\u4e24\u6b65\uff0c\u7532\u548c\u4e01\u90fd\u53d6\u96f6\u3002\u8fd9\u4e2a\u7ed3\u679c\u5f88\u5bb9\u6613\u753b\u51fa\u8fde\u7eed\u6e10\u53d8\uff0c\u4f46\u5df2\u4e0d\u80fd\u56de\u7b54\u201c\u4ece\u7532\u51fa\u53d1\u54ea\u6761\u5206\u652f\u51fa\u73b0\u5f97\u8f83\u665a\u201d\u3002\u56e0\u6b64\u628a\u5168\u90e8\u65e9\u671f\u6837\u672c\u673a\u68b0\u5730\u52a0\u5165\u6839\u540d\u5355\u4e4b\u524d\uff0c\u5148\u67e5\u770b\u8fd9\u4e9b\u5019\u9009\u843d\u5728\u4e3b\u56fe\u7684\u54ea\u4e9b\u4f4d\u7f6e\uff0c\u6807\u8bb0\u4e0d\u540c\u5019\u9009\u843d\u5728\u540c\u4e00\u7aef\u8fd8\u662f\u76f8\u5bf9\u7684\u4e24\u7aef\u3002<\/p>\n
\u5728\u9009\u6839\u548c\u9009\u5206\u652f\u4e4b\u95f4\u505a\u4e00\u6b21\u654f\u611f\u6027\u68c0\u67e5<\/h2>\n
\u5b9e\u64cd\u65f6\u53ef\u4ee5\u51c6\u5907\u4e24\u5957\u90fd\u6709\u4f9d\u636e\u7684\u6839\uff0c\u4e00\u5957\u4ec5\u53d6\u5f3a\u65e9\u671f\u6807\u8bb0\u7684\u7ec6\u80de\uff0c\u53e6\u4e00\u5957\u9002\u5ea6\u653e\u5bbd\u8fd9\u4e2a\u8303\u56f4\uff0c\u540c\u65f6\u56fa\u5b9a\u540c\u4e00\u5f20\u5df2\u5b66\u4e60\u7684\u4e3b\u56fe\u3002\u5bf9\u5171\u540c\u53ef\u8fbe\u7684\u7ec6\u80de\uff0c\u6bd4\u8f83\u7ec4\u522b\u987a\u5e8f\u3001\u5173\u952e\u57fa\u56e0\u6cbf\u7a0b\u66f2\u7ebf\u548c\u5019\u9009\u5206\u652f\u7aef\u70b9\u662f\u5426\u4fdd\u6301\u4e00\u81f4\u3002\u82e5\u4e00\u70b9\u70b9\u6539\u52a8\u5c31\u4f7f\u91cd\u8981\u7ed3\u8bba\u5012\u8fc7\u6765\uff0c\u5e94\u4fdd\u7559\u8fd9\u4e2a\u7ed3\u679c\uff0c\u628a\u201c\u65b9\u5411\u6682\u4e0d\u786e\u5b9a\u201d\u5199\u51fa\u6765\u3002\u82e5\u4e24\u5957\u90fd\u4e00\u81f4\uff0c\u4ecd\u8981\u56de\u5230\u5b9e\u9645\u53d6\u6750\u65f6\u95f4\u548c\u72ec\u7acb\u6807\u8bb0\u590d\u6838\uff0c\u5c24\u5176\u8981\u67e5\u67d0\u6761\u652f\u8def\u662f\u5426\u51e0\u4e4e\u5168\u90e8\u6765\u81ea\u5355\u4e00\u4f9b\u4f53\u3002<\/p>\n
\u4fdd\u5b58\u5206\u6790\u65f6\uff0c\u9664\u4e86\u4e3b\u56fe\u56fe\u7247\uff0c\u5e94\u4fdd\u7559\u6839\u7ec6\u80de\u7684\u6761\u5f62\u7801\u4e0e\u6837\u672c\u6765\u6e90\u3001\u5404\u5206\u533a\u53ef\u8fbe\u7ec6\u80de\u6570\uff0c\u4ee5\u53ca\u652f\u6301\u65e9\u665a\u987a\u5e8f\u7684\u5916\u90e8\u4f9d\u636e\u3002\u540e\u6765\u60f3\u590d\u6838\u67d0\u4e2a\u5019\u9009\u57fa\u56e0\u65f6\uff0c\u53ef\u4ee5\u5148\u9501\u5b9a\u540c\u4e00\u652f\u3001\u540c\u4e00\u6279\u5171\u540c\u7ec6\u80de\uff0c\u518d\u68c0\u67e5\u5728\u4e0d\u540c\u6839\u65b9\u6848\u4e0b\u7684\u8d8b\u52bf\u3002\u8fd9\u6bd4\u76f4\u63a5\u62ff\u4e24\u5f20\u4e0d\u540c\u6570\u636e\u5b50\u96c6\u3001\u4e0d\u540c\u5750\u6807\u548c\u4e0d\u540c\u6839\u7684\u8f68\u8ff9\u56fe\u6bd4\u8f83\u53ef\u9760\u5f97\u591a\u3002\u5982\u679c\u591a\u4f4d\u540c\u4e8b\u9700\u8981\u5728\u540c\u4e00\u56fe\u8c31\u4e0a\u590d\u8dd1\u8fd9\u4e9b\u5bf9\u7167\uff0c\u53ef\u4e86\u89e3\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668<\/a>\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u7528\u4e94\u4e2a\u8282\u70b9\u7b97\u6e05\u6362\u6839\u5982\u4f55\u6539\u53d8\u987a\u5e8f\uff0c\u5c55\u793a\u600e\u6837\u6309\u5206\u533a\u8ffd\u67e5\u65e0\u7a77\u62df\u65f6\u5e8f\uff0c\u5e76\u7528\u53d6\u6750\u65f6\u95f4\u6821\u9a8c\u65b9\u5411\u3002<\/p>\n","protected":false},"author":1,"featured_media":68075,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49,71],"tags":[661,662,663],"class_list":["post-68076","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","category-single-cell","tag-monocle-3","tag-662","tag-663"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/monocle3-root-partition-infinite-pseudotime-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/68076","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=68076"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/68076\/revisions"}],"predecessor-version":[{"id":68080,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/68076\/revisions\/68080"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/68075"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=68076"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=68076"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=68076"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}