{"id":68106,"date":"2026-11-14T20:18:29","date_gmt":"2026-11-14T12:18:29","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=68106"},"modified":"2026-11-14T21:06:05","modified_gmt":"2026-11-14T13:06:05","slug":"scvelo-splicing-phase-portrait-velocity-direction","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/scvelo-splicing-phase-portrait-velocity-direction\/","title":{"rendered":"scVelo \u7684\u7bad\u5934\u671d\u54ea\u513f\uff1f\u7528\u4e00\u5f20\u526a\u63a5\u76f8\u56fe\u68c0\u67e5 RNA \u901f\u5ea6"},"content":{"rendered":"
\n

\u5728\u4e00\u5f20\u80f0\u817a\u53d1\u80b2\u56fe\u4e0a\uff0c\u4e24\u7c07\u7ec6\u80de\u4e4b\u95f4\u753b\u7740\u4ece\u5de6\u5411\u53f3\u7684\u6d41\u7ebf\u3002\u8981\u5411\u5b9e\u9a8c\u540c\u4e8b\u89e3\u91ca\u5b83\uff0c\u5148\u56de\u5230\u4e00\u4e2a\u66f4\u5c0f\u7684\u95ee\u9898\u3002\u5bf9\u5176\u4e2d\u4e00\u4e2a\u57fa\u56e0\uff0c\u4e3a\u4ec0\u4e48\u6a21\u578b\u4f30\u8ba1\u6210\u719f\u8f6c\u5f55\u672c\u6b63\u5728\u589e\u52a0\uff1f\u53ea\u8981\u80fd\u753b\u51fa\u8fd9\u4e00\u4e2a\u57fa\u56e0\u7684\u526a\u63a5\u76f8\u56fe\uff0c\u518d\u68c0\u67e5\u54ea\u4e9b\u7ec6\u80de\u548c\u6837\u672c\u652f\u6301\u8fd9\u4e2a\u8d8b\u52bf\uff0c\u5c31\u6709\u529e\u6cd5\u533a\u5206\u4e00\u6761\u503c\u5f97\u9a8c\u8bc1\u7684\u53d1\u80b2\u5047\u8bf4\u548c\u4e00\u6761\u7eaf\u7cb9\u597d\u770b\u7684\u6d41\u7ebf\u3002\u4e0b\u9762\u4ece\u8bfb\u5165\u7684\u4e24\u5c42\u8ba1\u6570\u5f00\u59cb\uff0c\u4e00\u76f4\u7b97\u5230\u7bad\u5934\u6539\u53d8\u65b9\u5411\u7684\u6761\u4ef6\u3002<\/p>\n

\u4ece\u4e24\u5c42\u8ba1\u6570\u8ffd\u6eaf\u5230\u540c\u4e00\u4e2a\u7ec6\u80de<\/h2>\n

\u5e38\u7528\u7684\u5355\u7ec6\u80de\u8868\u8fbe\u77e9\u9635\u7ed9\u6bcf\u4e2a\u57fa\u56e0\u4e00\u4e2a\u603b\u91cf\u3002RNA \u901f\u5ea6\u8fd8\u9700\u8981\u533a\u5206\u5df2\u526a\u63a5\u548c\u672a\u526a\u63a5\u5206\u5b50\uff0c\u5e38\u4fdd\u5b58\u5728\u5bf9\u8c61\u7684 spliced<\/code> \u548c unspliced<\/code> \u4e24\u5c42\u3002\u603b\u91cf\u76f8\u540c\u7684\u4e24\u4e2a\u7ec6\u80de\u53ef\u80fd\u5177\u6709\u4e0d\u540c\u7684\u526a\u63a5\u6784\u6210\u3002\u53ea\u6709\u4e00\u5f20\u666e\u901a\u8868\u8fbe\u8868\u65f6\uff0c\u65e0\u6cd5\u4ece\u4e00\u4e2a\u603b\u6570\u552f\u4e00\u62c6\u5206\u51fa\u8fd9\u4e24\u79cd\u5206\u5b50\u3002\u63a5\u624b\u6570\u636e\u5148\u95ee\u6e05\u4e0a\u6e38\u5982\u4f55\u5b9a\u4e49\u5916\u663e\u5b50\u3001\u5185\u542b\u5b50\u548c\u6a21\u7cca\u8bfb\u6bb5\uff0c\u518d\u67e5\u4e24\u5c42\u662f\u4e0d\u662f\u6765\u81ea\u540c\u4e00\u6587\u5e93\u3001\u540c\u4e00\u7248\u6ce8\u91ca\u548c\u540c\u4e00\u7ec4\u6761\u5f62\u7801\u3002scVelo \u7684\u57fa\u7840\u6559\u7a0b<\/a><\/p>\n

\u5408\u5e76\u5df2\u6709\u56fe\u8c31\u548c\u901f\u5ea6\u6587\u4ef6\u65f6\uff0c\u5148\u5728\u6bcf\u4efd\u6587\u5e93\u5185\u6838\u5bf9\u6761\u5f62\u7801\uff0c\u518d\u6838\u5bf9\u57fa\u56e0\u7684\u7a33\u5b9a\u7f16\u53f7\u3002\u4e0d\u540c\u6587\u5e93\u53ef\u80fd\u91cd\u590d\u4f7f\u7528\u540c\u4e00\u4e2a\u6761\u5f62\u7801\uff0c\u5355\u7eaf\u5220\u6389\u6587\u5e93\u524d\u7f00\u4f1a\u8ba9\u672c\u6765\u5e94\u5f53\u5206\u5f00\u7684\u7ec6\u80de\u91cd\u540d\u3002\u6309\u6587\u5e93\u548c\u7ec6\u80de\u7c7b\u578b\u5206\u522b\u7edf\u8ba1\u4e24\u5c42\u4e2d\u975e\u96f6\u5206\u5b50\u7684\u6570\u91cf\u3002\u67d0\u4e00\u6574\u6279\u51e0\u4e4e\u6ca1\u6709\u672a\u526a\u63a5\u5206\u5b50\uff0c\u4f18\u5148\u68c0\u67e5\u5efa\u5e93\u65b9\u6848\u3001\u53c2\u8003\u6ce8\u91ca\u548c\u4e0a\u6e38\u8ba1\u6570\u65b9\u5f0f\u3002\u628a\u8fd9\u6837\u7684\u6279\u6b21\u4e0e\u5176\u4ed6\u6279\u6b21\u4e00\u8d77\u5e73\u6ed1\uff0c\u90bb\u5c45\u7684\u652f\u6301\u53ef\u80fd\u63a9\u76d6\u5b83\u539f\u672c\u7f3a\u5c11\u89c2\u6d4b\u8fd9\u4e00\u4e8b\u5b9e\u3002<\/p>\n

\u624b\u7b97\u4e00\u6761\u57fa\u56e0\u7684\u65b9\u5411<\/h2>\n

\u8bbe\u5728\u7b80\u5316\u6a21\u578b\u4e2d\uff0c\u6210\u719f\u5206\u5b50\u7684\u77ac\u65f6\u53d8\u5316\u53d6\u51b3\u4e8e\u672a\u526a\u63a5\u5206\u5b50\u7684\u52a0\u5de5\u6d41\u5165\u91cf\uff0c\u51cf\u53bb\u6210\u719f\u5206\u5b50\u7684\u964d\u89e3\u6d41\u51fa\u91cf\u3002\u7528\u540c\u4e00\u5957\u5f52\u4e00\u5316\u5355\u4f4d\u505a\u4e00\u4e2a\u6559\u5b66\u7b97\u4f8b\uff0c\u4ee4\u52a0\u5de5\u901f\u7387\u4e3a\u4e00\u3001\u964d\u89e3\u901f\u7387\u4e3a\u4e8c\u5206\u4e4b\u4e00\u3002\u5982\u679c\u5f53\u524d\u6709\u5341\u4e2a\u5355\u4f4d\u7684\u6210\u719f\u5206\u5b50\uff0c\u90a3\u4e48\u6bcf\u4e2a\u65f6\u95f4\u5355\u4f4d\u6d41\u51fa\u7684\u91cf\u4e3a\u4e94\u3002\u6b64\u65f6\u672a\u526a\u63a5\u91cf\u4e3a\u516b\uff0c\u51c0\u53d8\u5316\u5c31\u662f\u6b63\u4e09\u3002\u82e5\u672a\u526a\u63a5\u91cf\u4e3a\u4e8c\uff0c\u51c0\u53d8\u5316\u5c31\u662f\u8d1f\u4e09\u3002\u4e24\u79cd\u60c5\u51b5\u91cc\u672a\u526a\u63a5\u91cf\u90fd\u4f4e\u4e8e\u6210\u719f\u91cf\uff0c\u53d8\u5316\u65b9\u5411\u5374\u6070\u597d\u76f8\u53cd\u3002<\/p>\n

\n\n\n\n\n\n\n\n
\u6210\u719f\u91cf<\/th>\n\u672a\u526a\u63a5\u91cf<\/th>\n\u52a0\u5de5\u6d41\u5165\u51cf\u964d\u89e3\u6d41\u51fa<\/th>\n\u8fd9\u4e00\u57fa\u56e0\u7684\u8d8b\u52bf<\/th>\n<\/tr>\n<\/thead>\n
10<\/td>\n8<\/td>\n8 \u2212 5 = 3<\/td>\n\u6210\u719f\u91cf\u5728\u8be5\u70b9\u8d8b\u4e8e\u4e0a\u5347<\/td>\n<\/tr>\n
10<\/td>\n5<\/td>\n5 \u2212 5 = 0<\/td>\n\u6a21\u578b\u7ed9\u51fa\u7684\u77ac\u65f6\u51c0\u53d8\u5316\u4e3a\u96f6<\/td>\n<\/tr>\n
10<\/td>\n2<\/td>\n2 \u2212 5 = \u22123<\/td>\n\u6210\u719f\u91cf\u5728\u8be5\u70b9\u8d8b\u4e8e\u4e0b\u964d<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u4e3a\u4ec0\u4e48\u5c11\u4e8e\u5341\uff0c\u4e5f\u80fd\u5411\u4e0a\u8d70\u3002\u6559\u5b66\u7b97\u4f8b\uff5c\u6210\u719f\u91cf\u4e3a\u5341\uff0c\u964d\u89e3\u6d41\u51fa\u4e3a\u4e94\uff0c\u52a0\u5de5\u6d41\u5165\u968f\u672a\u526a\u63a5\u91cf\u6539\u53d8\u30028
\u6559\u5b66\u7b97\u4f8b\uff5c\u6210\u719f\u91cf\u4e3a\u5341\uff0c\u964d\u89e3\u6d41\u51fa\u4e3a\u4e94\uff0c\u52a0\u5de5\u6d41\u5165\u968f\u672a\u526a\u63a5\u91cf\u6539\u53d8\u3002<\/figcaption><\/figure>\n

\u5bf9\u5e94\u5230\u76f8\u56fe\uff0c\u628a\u6210\u719f\u91cf\u653e\u5728\u6a2a\u8f74\uff0c\u672a\u526a\u63a5\u91cf\u653e\u5728\u7eb5\u8f74\u3002\u5728\u8fd9\u4e2a\u7b97\u4f8b\u91cc\uff0c\u51c0\u53d8\u5316\u4e3a\u96f6\u7684\u7ebf\u7a7f\u8fc7\u6a2a\u5750\u6807\u5341\u3001\u7eb5\u5750\u6807\u4e94\u7684\u4f4d\u7f6e\u3002\u70b9\u5728\u8fd9\u6761\u7ebf\u4e0a\u65b9\u8fd8\u662f\u4e0b\u65b9\uff0c\u6bd4\u76f4\u63a5\u6bd4\u8f83\u4e24\u4e2a\u8f74\u7684\u6570\u503c\u5927\u5c0f\u66f4\u6709\u610f\u4e49\u3002\u771f\u5b9e\u52a8\u529b\u5b66\u5206\u6790\u4f1a\u4f30\u8ba1\u57fa\u56e0\u7279\u5f02\u7684\u901f\u7387\u3001\u5c3a\u5ea6\u548c\u6f5c\u5728\u72b6\u6001\uff0c\u4e0d\u80fd\u5c06\u6559\u5b66\u4f8b\u4e2d\u7684\u659c\u7387\u5f53\u4f5c\u8f6f\u4ef6\u7684\u56fa\u5b9a\u5e38\u6570\u3002\u770b\u5b9e\u9645\u76f8\u56fe\u65f6\uff0c\u628a\u7528\u4e8e\u62df\u5408\u7684\u7ec6\u80de\u7740\u8272\uff0c\u7559\u610f\u6b63\u8d1f\u8d8b\u52bf\u662f\u5426\u6709\u4e00\u7247\u8fde\u7eed\u7684\u7ec6\u80de\u652f\u6301\uff0c\u8fd8\u662f\u4ec5\u9760\u8fb9\u7f18\u51e0\u4e2a\u5f02\u5e38\u70b9\u51b3\u5b9a\u3002<\/p>\n

\u770b\u5e73\u6ed1\u662f\u5728\u8c01\u548c\u8c01\u4e4b\u95f4\u8fdb\u884c<\/h2>\n

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\u4e0b\u9762\u7528\u5185\u7f6e\u80f0\u817a\u5bf9\u8c61\u8bf4\u660e\uff0c\u5148\u68c0\u67e5\u4e24\u5c42\u662f\u5426\u5b58\u5728\uff0c\u518d\u505a\u7b5b\u9009\u3001\u8ba1\u7b97\u90bb\u57df\u77e9\u3001\u62df\u5408\u52a8\u529b\u5b66\u548c\u6784\u5efa\u901f\u5ea6\u56fe\u3002\u793a\u4f8b\u7684\u8fd1\u90bb\u6570\u7528\u4e8e\u6f14\u793a\u3002\u5bf9\u5c0f\u7684\u5019\u9009\u8c31\u7cfb\uff0c\u5e94\u68c0\u67e5\u5269\u4f59\u7ec6\u80de\u6570\u548c\u6570\u636e\u7ef4\u5ea6\uff0c\u518d\u9009\u62e9\u6709\u610f\u4e49\u7684\u5c40\u90e8\u5c3a\u5ea6\u3002\u5df2\u6267\u884c\u8fd9\u4e9b\u5904\u7406\u7684\u5bf9\u8c61\u53e6\u5b58\u4e00\u4efd\uff0c\u907f\u514d\u5728\u53d6\u5bf9\u6570\u540e\u7684\u6570\u636e\u4e0a\u91cd\u65b0\u8dd1\u4e00\u904d\u9884\u5904\u7406\u3002\u52a8\u529b\u5b66\u62df\u5408\u53ca\u8f93\u51fa<\/a><\/p>\n

import scvelo as scv\r\n\r\nadata = scv.datasets.pancreas()\r\nassert {"spliced", "unspliced"} <= set(adata.layers)\r\nscv.pp.filter_and_normalize(adata, min_shared_counts=20)\r\nscv.pp.moments(adata, n_pcs=30, n_neighbors=30)\r\nscv.tl.recover_dynamics(adata)\r\nscv.tl.velocity(adata, mode="dynamical")\r\nscv.tl.velocity_graph(adata)\r\n<\/code><\/pre>\n
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