{"id":68112,"date":"2026-11-14T20:18:37","date_gmt":"2026-11-14T12:18:37","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=68112"},"modified":"2026-11-14T20:18:37","modified_gmt":"2026-11-14T12:18:37","slug":"cellchat-ligand-receptor-missing-edge-trimean","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/cellchat-ligand-receptor-missing-edge-trimean\/","title":{"rendered":"CellChat \u5c11\u4e86\u4e00\u6761\u914d\u4f53\u2013\u53d7\u4f53\u8fde\u7ebf\uff0c\u53bb\u54ea\u91cc\u627e\u539f\u56e0\uff1f"},"content":{"rendered":"<div class=\"ysx-method-guide\" style=\"max-width:820px;min-width:0;margin:0 auto;color:#26374a;line-height:1.86;overflow-wrap:anywhere\">\n<p>\u4e00\u5f20\u6cbb\u7597\u524d\u540e\u7684\u7ec6\u80de\u901a\u4fe1\u56fe\u4e0a\uff0c\u6210\u7ea4\u7ef4\u7ec6\u80de\u6307\u5411\u514d\u75ab\u7ec6\u80de\u7684\u4e00\u6761\u7ebf\u6d88\u5931\u4e86\u3002\u540c\u4e8b\u60f3\u77e5\u9053\u662f\u4e0d\u662f\u6cbb\u7597\u963b\u65ad\u4e86\u8fd9\u6761\u901a\u8def\u3002\u6253\u5f00\u4e24\u7ec4\u5bf9\u8c61\uff0c\u53ef\u80fd\u53d1\u73b0\u6cbb\u7597\u540e\u6839\u672c\u6ca1\u91c7\u5230\u8db3\u591f\u591a\u7684\u63a5\u6536\u7ec6\u80de\uff0c\u4e5f\u53ef\u80fd\u53d1\u73b0\u914d\u4f53\u660e\u660e\u8fd8\u6709\u4eba\u8868\u8fbe\uff0c\u53ea\u662f\u8868\u8fbe\u7ec6\u80de\u8fc7\u5c11\uff0c\u6309\u7ec4\u6c47\u603b\u540e\u53d8\u6210\u4e86\u96f6\u3002\u8fd9\u51e0\u79cd\u60c5\u51b5\u5728\u5706\u5708\u56fe\u4e0a\u770b\u8d77\u6765\u4e00\u6837\uff0c\u5374\u9700\u8981\u5b8c\u5168\u4e0d\u540c\u7684\u540e\u7eed\u5b9e\u9a8c\u3002\u8ffd\u67e5\u4e00\u6761\u7ebf\u65f6\uff0c\u628a\u53d1\u9001\u7fa4\u3001\u63a5\u6536\u7fa4\u3001\u5177\u4f53\u914d\u4f53\u548c\u53d7\u4f53\u540c\u65f6\u56fa\u5b9a\u4e0b\u6765\uff0c\u80fd\u7701\u6389\u8bb8\u591a\u6765\u56de\u8bd5\u53c2\u6570\u7684\u65f6\u95f4\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5148\u7ed9\u4e00\u6761\u8fb9\u627e\u5230\u56db\u4e2a\u540d\u5b57<\/h2>\n<p>\u8bbe\u60f3\u8981\u8ffd\u67e5\u7684\u662f\u6210\u7ea4\u7ef4\u7ec6\u80de\u4e2d\u7684\u914d\u4f53\u548c\u67d0\u4e00\u7c7b\u514d\u75ab\u7ec6\u80de\u4e0a\u7684\u53d7\u4f53\u3002\u7b2c\u4e00\u6b65\u5148\u5217\u51fa\u4e24\u7ec4\u7684\u7ec6\u80de\u7c7b\u578b\u6807\u7b7e\u548c\u6bcf\u4f4d\u4f9b\u4f53\u5404\u6709\u591a\u5c11\u7ec6\u80de\u3002\u5982\u679c\u6cbb\u7597\u524d\u628a\u514d\u75ab\u7ec6\u80de\u7ec6\u5206\u6210\u56db\u7fa4\uff0c\u6cbb\u7597\u540e\u5374\u53ea\u4fdd\u7559\u4e00\u4e2a\u5927\u7c7b\uff0c\u90a3\u4e48\u5373\u4f7f\u540c\u4e00\u4e2a\u5c0f\u7fa4\u7684\u8868\u8fbe\u6ca1\u6709\u53d8\u5316\uff0c\u5408\u5e76\u5165\u66f4\u591a\u4e0d\u8868\u8fbe\u53d7\u4f53\u7684\u7ec6\u80de\u4e5f\u4f1a\u6539\u53d8\u5e73\u5747\u503c\u3002\u6700\u597d\u660e\u786e\u4e24\u4e2a\u6570\u636e\u96c6\u5171\u6709\u7684\u6ce8\u91ca\u5c42\u7ea7\uff0c\u4fdd\u7559\u539f\u59cb\u7ec6\u5206\u6807\u7b7e\uff0c\u518d\u5728\u540c\u4e00\u5957\u5b9a\u4e49\u4e0b\u6bd4\u8f83\u3002<\/p>\n<p>\u7b2c\u4e8c\u6b65\u68c0\u67e5\u6570\u636e\u5e93\u8bb0\u5f55\u3002\u4e00\u4e2a\u914d\u4f53\u2013\u53d7\u4f53\u5bf9\u6709\u65f6\u9700\u8981\u591a\u4e2a\u4e9a\u57fa\u5171\u540c\u6784\u6210\u53d7\u4f53\uff0c\u4e5f\u6709\u76f8\u4e92\u4f5c\u7528\u53d7\u5230\u534f\u540c\u56e0\u5b50\u7b49\u9879\u76ee\u5f71\u54cd\u3002\u53ea\u5728\u56fe\u4e0a\u627e\u5230\u4e86\u5176\u4e2d\u4e00\u4e2a\u663e\u773c\u7684\u53d7\u4f53\u57fa\u56e0\uff0c\u4e0d\u8db3\u4ee5\u8bf4\u660e\u8fd9\u4e2a\u590d\u5408\u7269\u5728\u63a5\u6536\u7fa4\u4e2d\u6709\u5145\u5206\u8868\u8fbe\u3002\u628a\u7269\u79cd\u3001\u6570\u636e\u5e93\u7248\u672c\u3001\u914d\u4f53\u548c\u5404\u4e2a\u5fc5\u8981\u4e9a\u57fa\u9010\u9879\u5217\u51fa\uff0c\u518d\u53bb\u770b\u8fd9\u4e9b\u57fa\u56e0\u7684\u68c0\u51fa\u5206\u5e03\u3002\u82e5\u539f\u59cb\u77e9\u9635\u7684\u7f16\u53f7\u65e0\u6cd5\u5bf9\u5e94\u6570\u636e\u5e93\uff0c\u5148\u5904\u7406\u540d\u79f0\u548c\u7269\u79cd\u5339\u914d\u3002<a style=\"color:#9a4659;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/jinworks\/CellChat\/blob\/main\/tutorial\/CellChat-vignette.Rmd\">CellChat \u4f5c\u8005\u7684\u5206\u6790\u6559\u7a0b<\/a><\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u4e00\u6837\u7684\u666e\u901a\u5747\u503c\uff0c\u4e3a\u4ec0\u4e48\u8f6f\u4ef6\u53ef\u80fd\u53ea\u753b\u51fa\u4e00\u6761\u7ebf<\/h2>\n<p>\u7528\u4e24\u4e2a\u5404\u6709\u4e8c\u5341\u679a\u53d1\u9001\u7ec6\u80de\u7684\u6559\u5b66\u6837\u672c\u6f14\u7b97\u3002\u7532\u7ec4\u56db\u679a\u7ec6\u80de\u6bcf\u679a\u8868\u8fbe\u4e94\u4e2a\u5355\u4f4d\uff0c\u5176\u4f59\u5341\u516d\u679a\u4e3a\u96f6\u3002\u4e59\u7ec4\u5341\u679a\u6bcf\u679a\u8868\u8fbe\u4e24\u4e2a\u5355\u4f4d\uff0c\u5176\u4f59\u5341\u679a\u4e3a\u96f6\u3002\u4e24\u7ec4\u666e\u901a\u5747\u503c\u90fd\u662f\u4e00\uff0c\u8868\u8fbe\u7ec6\u80de\u6bd4\u4f8b\u5374\u5206\u522b\u4e3a\u4e94\u5206\u4e4b\u4e00\u548c\u4e8c\u5206\u4e4b\u4e00\u3002\u5982\u679c\u53ea\u62a5\u544a\u5408\u5e76\u5747\u503c\uff0c\u8bfb\u8005\u770b\u4e0d\u5230\u662f\u54ea\u79cd\u683c\u5c40\u652f\u6491\u4e86\u4fe1\u53f7\u3002<\/p>\n<div class=\"guide-table\" style=\"max-width:100%;overflow-x:auto;margin:18px 0\">\n<table style=\"display:table;width:100%;max-width:100%;min-width:0;table-layout:fixed;border-collapse:collapse;font-size:.92em;line-height:1.6\">\n<thead>\n<tr>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#fff1f3;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u53d1\u9001\u7ec6\u80de\u7684\u60c5\u51b5<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#fff1f3;color:#1e293b;vertical-align:top;text-align:right;padding:6px;border:1px solid #c4cfdb\">\u7532\u7ec4<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#fff1f3;color:#1e293b;vertical-align:top;text-align:right;padding:6px;border:1px solid #c4cfdb\">\u4e59\u7ec4<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u68c0\u51fa\u7ec6\u80de \/ \u603b\u7ec6\u80de<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">4 \/ 20<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">10 \/ 20<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u6240\u6709\u7ec6\u80de\u7684\u666e\u901a\u5747\u503c<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">1<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">1<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u672c\u4f8b\u7684\u4e09\u5747\u503c<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">0<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<figure class=\"guide-figure\" style=\"margin:24px 0 28px\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" src=\"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/cellchat-ligand-receptor-missing-edge-trimean-guide.png?resize=1200%2C840&#038;ssl=1\" alt=\"\u666e\u901a\u5747\u503c\u76f8\u540c\uff0c\u8fde\u7ebf\u4e3a\u4f55\u6d88\u5931\u3002\u6559\u5b66\u7b97\u4f8b\uff5c\u6bcf\u7ec4\u4e8c\u5341\u4e2a\u53d1\u9001\u7ec6\u80de\uff0c\u8868\u8fbe\u603b\u91cf\u5747\u4e3a\u4e8c\u5341\u30024 \/ 20\uff0c\u7532\u7ec4\u56db\u4e2a\u9633\u6027\u7ec6\u80de\uff0c\u6bcf\u4e2a\u9633\u6027\u8868\u8fbe\u4e94\u4e2a\u5355\u4f4d\uff0c\u4e09\u5747\u503c\u4e3a\u96f6\u300210 \/ 20\uff0c\u4e59\u7ec4\u5341\u4e2a\u9633\u6027\u7ec6\u80de\uff0c\u6bcf\u4e2a\u9633\u6027\u8868\u8fbe\u4e24\u4e2a\u5355\u4f4d\uff0c\u4e09\u5747\u503c\u4e3a\u4e00\u3002\u56de\u67e5\uff0c\u63a5\u6536\u7fa4\u3001\u53d7\u4f53\u4e9a\u57fa\u3001\u4f9b\u4f53\uff0c\u5206\u5f00\u6838\u67e5\u7fa4\u4f53\u4e30\u5ea6\u548c\u914d\u4f53\u2013\u53d7\u4f53\u8868\u8fbe\u3002\" width=\"1200\" height=\"840\" style=\"display:block;width:100%;height:auto;border-radius:10px\"><figcaption style=\"font-size:14px;line-height:1.6;margin:9px 2px 0;color:#475569\">\u6559\u5b66\u7b97\u4f8b\uff5c\u6bcf\u7ec4\u4e8c\u5341\u4e2a\u53d1\u9001\u7ec6\u80de\uff0c\u8868\u8fbe\u603b\u91cf\u5747\u4e3a\u4e8c\u5341\u3002<\/figcaption><\/figure>\n<p>\u4e09\u5747\u503c\u7528\u7b2c\u4e00\u56db\u5206\u4f4d\u6570\u3001\u4e24\u500d\u4e2d\u4f4d\u6570\u548c\u7b2c\u4e09\u56db\u5206\u4f4d\u6570\u76f8\u52a0\uff0c\u518d\u9664\u4ee5\u56db\u3002\u7532\u7ec4\u8fd9\u4e09\u4e2a\u4f4d\u7f6e\u90fd\u5728\u96f6\u503c\u533a\u3002\u4e59\u7ec4\u5219\u5206\u522b\u662f\u96f6\u3001\u4e00\u548c\u4e8c\uff0c\u653e\u5165\u516c\u5f0f\u4e3a\u4e00\u3002\u8fd9\u4e2a\u4f8b\u5b50\u53ea\u53d6\u51fa\u7ec4\u8868\u8fbe\u6c47\u603b\u8fd9\u4e00\u6b65\uff0c\u8f6f\u4ef6\u5b8c\u6574\u7684\u901a\u4fe1\u6982\u7387\u8fd8\u7528\u5230\u63a5\u6536\u7aef\u8868\u8fbe\u3001\u6570\u636e\u5e93\u5173\u7cfb\u548c\u8bbe\u7f6e\u7b49\u6761\u4ef6\uff0c\u4e0d\u80fd\u628a\u8868\u4e2d\u6700\u540e\u4e00\u884c\u7406\u89e3\u6210\u6700\u7ec8\u7ed8\u7ebf\u7684\u901a\u4fe1\u6982\u7387\u3002\u5b83\u89e3\u91ca\u7684\u662f\uff0c\u6070\u597d\u5728\u5c11\u6570\u7ec6\u80de\u8868\u8fbe\u7684\u914d\u4f53\uff0c\u5373\u4f7f\u9633\u6027\u7ec6\u80de\u7684\u5206\u5b50\u91cf\u5f88\u53ef\u89c2\uff0c\u4e5f\u6709\u53ef\u80fd\u5728\u76f8\u5bf9\u7a33\u5065\u7684\u6c47\u603b\u4e2d\u6d88\u5931\u3002<a style=\"color:#9a4659;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/jinworks\/CellChat\/blob\/main\/R\/modeling.R\">CellChat \u6a21\u578b\u4e0e\u7ec4\u5185\u8868\u8fbe\u8ba1\u7b97<\/a><\/p>\n<p>\u53ef\u4ee5\u7528\u4e0b\u9762\u7684\u77ed\u811a\u672c\u6838\u5bf9\u8fd9\u7ec4\u5177\u4f53\u6570\u503c\u3002\u62ff\u5230\u771f\u5b9e\u6570\u636e\u65f6\uff0c\u4e5f\u53ef\u628a\u540c\u4e00\u914d\u4f53\u5728\u6bcf\u4f4d\u4f9b\u4f53\u5185\u7684\u666e\u901a\u5747\u503c\u3001\u7a33\u5065\u5747\u503c\u548c\u68c0\u51fa\u6bd4\u4f8b\u653e\u5728\u4e00\u5f20\u8868\u91cc\u3002\u5982\u679c\u67d0\u6761\u8fb9\u53ea\u5728\u6362\u6210\u8f83\u5bbd\u677e\u7684\u622a\u5c3e\u5747\u503c\u540e\u51fa\u73b0\uff0c\u628a\u4e24\u79cd\u7ed3\u679c\u540c\u65f6\u5c55\u793a\uff0c\u5e76\u56de\u770b\u662f\u5426\u53ea\u7531\u51e0\u4e2a\u7ec6\u80de\u8d21\u732e\uff0c\u6bd4\u6311\u51fa\u6709\u7ebf\u7684\u90a3\u5f20\u56fe\u66f4\u6709\u89e3\u91ca\u529b\u3002<\/p>\n<pre style=\"max-width:100%;box-sizing:border-box;overflow-x:auto;margin:18px 0;padding:14px 16px;background:#f3f6fa;color:#1a293c;border:1px solid #d8e1ea;border-radius:8px;line-height:1.55;white-space:pre;overflow-wrap:normal;word-break:normal\"><code class=\"language-r\">a &lt;- c(rep(0, 16), rep(5, 4))\r\nb &lt;- c(rep(0, 10), rep(2, 10))\r\ntrimean &lt;- function(x) {\r\n  q &lt;- quantile(x, c(.25, .5, .75), names = FALSE)\r\n  sum(q * c(1, 2, 1)) \/ 4\r\n}\r\nrbind(A = c(mean(a), trimean(a)), B = c(mean(b), trimean(b)))\r\n<\/code><\/pre>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 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