{"id":68218,"date":"2026-11-14T20:25:00","date_gmt":"2026-11-14T12:25:00","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=68218"},"modified":"2026-11-14T20:49:22","modified_gmt":"2026-11-14T12:49:22","slug":"singler-pruned-labels-median-score-missing-reference","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/singler-pruned-labels-median-score-missing-reference\/","title":{"rendered":"SingleR \u7ed9\u51fa\u4e86\u7ec6\u80de\u6807\u7b7e\uff0c\u600e\u6837\u8bc6\u522b\u90a3\u4e9b\u5e94\u8be5\u6682\u7559\u7a7a\u767d\u7684\u7ec6\u80de\uff1f"},"content":{"rendered":"
\n

\u7ed9\u4e00\u6279\u65b0\u6837\u672c\u505a\u53c2\u8003\u6ce8\u91ca\uff0c\u7ed3\u679c\u8868\u91cc\u6bcf\u4e00\u884c\u90fd\u6709\u4e00\u4e2a\u7ec6\u80de\u7c7b\u578b\uff0c\u5408\u4f5c\u8005\u6253\u7b97\u76f4\u63a5\u636e\u6b64\u8ba1\u7b97\u5404\u7c7b\u578b\u7684\u6bd4\u4f8b\u3002\u503c\u5f97\u5148\u770b\u4e00\u773c\u53e6\u4e00\u5217\u3002SingleR \u53ef\u4ee5\u5728\u539f\u59cb\u5206\u914d\u6807\u7b7e\u4e4b\u5916\u7ed9\u51fa\u7ecf\u8fc7\u4fee\u526a\u7684\u6807\u7b7e\uff0c\u90e8\u5206\u7ec6\u80de\u5728\u8fd9\u4e00\u5217\u4f1a\u53d8\u6210\u7f3a\u5931\u503c\u3002\u4fdd\u7559\u8fd9\u4e9b\u7f3a\u5931\u53ca\u5176\u539f\u56e0\uff0c\u80fd\u8ba9\u6700\u7ec8\u7684\u7ec4\u6210\u8868\u66f4\u53ef\u4fe1\u3002\u8fd9\u91cc\u7528\u624b\u7b97\u7684\u4e09\u4e2a\u7ec6\u80de\u8bf4\u660e\uff0c\u4e3a\u4ec0\u4e48\u201c\u5206\u6570\u6700\u9ad8\u201d\u53ea\u89e3\u51b3\u4e86\u6bd4\u8f83\u4e2d\u7684\u4e00\u534a\u95ee\u9898\u3002<\/p>\n

\u5148\u67e5\u53c2\u8003\u80fd\u5426\u56de\u7b54\u4f60\u7684\u95ee\u9898<\/h2>\n

\u628a\u53c2\u8003\u6570\u636e\u7684\u7269\u79cd\u3001\u7ec4\u7ec7\u3001\u5e74\u9f84\u6216\u75be\u75c5\u72b6\u6001\u3001\u6d4b\u5e8f\u6280\u672f\u548c\u6807\u7b7e\u5c42\u7ea7\u5199\u4e0b\u6765\u3002\u4e00\u4e2a\u53ea\u6536\u5f55\u5916\u5468\u514d\u75ab\u7ec6\u80de\u7684\u53c2\u8003\u5f88\u597d\u7528\uff0c\u4f46\u9047\u5230\u5b9e\u4f53\u7ec4\u7ec7\u91cc\u7684\u5185\u76ae\u6216\u4e0a\u76ae\u7ec6\u80de\uff0c\u5b83\u6240\u80fd\u9009\u62e9\u7684\u7c7b\u522b\u6709\u9650\u3002\u67d0\u4e2a\u6807\u7b7e\u5373\u4f7f\u8dd1\u5f97\u5f88\u6574\u9f50\uff0c\u4e5f\u53ef\u80fd\u53ea\u662f\u6bcf\u4e2a\u7ec6\u80de\u90fd\u5728\u540c\u4e00\u7ec4\u4e0d\u5408\u9002\u7684\u5019\u9009\u91cc\u9009\u4e86\u6700\u63a5\u8fd1\u7684\u4e00\u4e2a\u3002\u5148\u7528\u7ec4\u7ec7\u5b9a\u4f4d\u548c\u4e3b\u8981\u8c31\u7cfb\u6807\u8bb0\u68c0\u67e5\u6709\u65e0\u660e\u663e\u7f3a\u5e2d\u7c7b\u522b\uff0c\u518d\u51b3\u5b9a\u6ce8\u91ca\u5230\u5927\u7c7b\u8fd8\u662f\u4e9a\u7c7b\u3002<\/p>\n

SingleR \u4ece\u53c2\u8003\u6570\u636e\u4e2d\u627e\u53ef\u5206\u8fa8\u4e0d\u540c\u6807\u7b7e\u7684\u7279\u5f81\uff0c\u5728\u8fd9\u4e9b\u7279\u5f81\u4e0a\u6bd4\u8f83\u5f85\u6ce8\u91ca\u7ec6\u80de\u4e0e\u53c2\u8003\u8868\u8fbe\u8c31\uff0c\u5178\u578b\u6d41\u7a0b\u8fd8\u4f1a\u5728\u76f8\u8fd1\u5019\u9009\u4e2d\u7ec6\u5316\u5339\u914d\u3002\u53c2\u8003\u6807\u7b7e\u6709\u591a\u5c11\u4e2a\u4f8b\u5b50\u3001\u540c\u540d\u6807\u7b7e\u5185\u90e8\u662f\u5426\u5305\u542b\u4e0d\u540c\u72b6\u6001\u3001\u8f93\u5165\u53cc\u65b9\u6709\u591a\u5c11\u53ef\u7528\u6807\u8bb0\uff0c\u90fd\u4f1a\u5f71\u54cd\u7ed3\u679c\u3002\u5f00\u59cb\u524d\u68c0\u67e5\u53cc\u65b9\u57fa\u56e0\u540d\u79f0\u7684\u4ea4\u96c6\uff0c\u5c24\u5176\u770b\u7528\u4e8e\u533a\u5206\u8fd1\u4eb2\u4e9a\u578b\u7684\u6807\u8bb0\u662f\u5426\u5728\u67e5\u8be2\u5b9e\u9a8c\u4e2d\u786e\u5b9e\u53ef\u6d4b\u3002\u53ea\u6709\u5171\u6709\u7684\u7ba1\u5bb6\u57fa\u56e0\u5f88\u591a\uff0c\u5e76\u4e0d\u80fd\u8bf4\u660e\u5177\u6709\u533a\u5206\u529b\u7684\u57fa\u56e0\u4e5f\u8db3\u591f\u3002SingleR \u4f5c\u8005\u5bf9\u7ecf\u5178\u6a21\u5f0f\u7684\u8bf4\u660e<\/a><\/p>\n

\u7b97\u4e00\u904d\u4e0e\u4e2d\u4f4d\u5206\u7684\u5dee\uff0c\u522b\u628a\u5b83\u8bfb\u6210\u6982\u7387<\/h2>\n

\u5047\u8bbe\u53c2\u8003\u6709\u7532\u3001\u4e59\u3001\u4e19\u3001\u4e01\u3001\u620a\u4e94\u79cd\u6807\u7b7e\uff0c\u4e0b\u8868\u662f\u4e3a\u8bf4\u660e\u539f\u7406\u6784\u9020\u7684\u5206\u6570\uff0c\u5e76\u5047\u5b9a\u6700\u7ec8\u5206\u914d\u90fd\u662f\u7532\u3002\u4f5c\u8005\u5728\u6ce8\u91ca\u8bca\u65ad\u4e2d\u5b9a\u4e49\u4e86\u4e00\u79cd\u5dee\u503c\uff0c\u7528\u5206\u914d\u6807\u7b7e\u7684\u5206\u6570\u51cf\u53bb\u672c\u7ec6\u80de\u5728\u5168\u90e8\u6807\u7b7e\u4e0a\u7684\u5206\u6570\u4e2d\u4f4d\u6570\u3002\u8fd9\u4e2a\u91cf\u8003\u5bdf\u88ab\u5206\u914d\u6807\u7b7e\u6bd4\u4e00\u822c\u5019\u9009\u7a81\u51fa\u4e86\u591a\u5c11\u3002\u4e0e\u4e4b\u4e0d\u540c\uff0c\u7b2c\u4e00\u540d\u51cf\u7b2c\u4e8c\u540d\u56de\u7b54\u7684\u662f\u524d\u4e24\u540d\u4e4b\u95f4\u6709\u591a\u96be\u533a\u5206\u3002\u4e24\u79cd\u95ee\u9898\u5e38\u5728\u70ed\u56fe\u6c47\u62a5\u4e2d\u88ab\u6df7\u5728\u4e00\u8d77\u3002<\/p>\n

\n\n\n\n\n\n\n\n
\u7ec6\u80de<\/th>\n\u7532\u3001\u4e59\u3001\u4e19\u3001\u4e01\u3001\u620a\u7684\u5206\u6570<\/th>\n\u7532\u51cf\u4e2d\u4f4d\u6570<\/th>\n\u7532\u51cf\u4e59<\/th>\n<\/tr>\n<\/thead>\n
\u4e00<\/td>\n0.80\u30010.40\u30010.30\u30010.20\u30010.10<\/td>\n0.50<\/td>\n0.40<\/td>\n<\/tr>\n
\u4e8c<\/td>\n0.80\u30010.79\u30010.30\u30010.20\u30010.10<\/td>\n0.50<\/td>\n0.01<\/td>\n<\/tr>\n
\u4e09<\/td>\n0.44\u30010.43\u30010.42\u30010.40\u30010.38<\/td>\n0.02<\/td>\n0.01<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u540c\u6837\u9ad8\u51fa\u4e2d\u4f4d\u6570\uff0c\u4e5f\u53ef\u80fd\u96be\u4ee5\u5b9a\u540d\u3002\u4e09\u4e2a\u6559\u5b66\u7ec6\u80de\u90fd\u5c06\u7532\u6392\u5728\u9996\u4f4d\uff0c\u6bd4\u8f83\u7532\u4e0e\u5176\u4f59\u6807\u7b7e\u7684\u5dee\u8ddd\u3002\u7ec6\u80de\u4e00\uff0c\u7532\u51cf\u4e2d\u4f4d\u6570
\u4e09\u4e2a\u6559\u5b66\u7ec6\u80de\u90fd\u5c06\u7532\u6392\u5728\u9996\u4f4d\uff0c\u6bd4\u8f83\u7532\u4e0e\u5176\u4f59\u6807\u7b7e\u7684\u5dee\u8ddd\u3002<\/figcaption><\/figure>\n

\u7b2c\u4e00\u679a\u4e0e\u7b2c\u4e8c\u679a\u5728\u201c\u7532\u6bd4\u4e00\u822c\u5019\u9009\u597d\u591a\u5c11\u201d\u8fd9\u4e2a\u95ee\u9898\u4e0a\u5f97\u51fa\u76f8\u540c\u7b54\u6848\uff0c\u7b2c\u4e8c\u679a\u5374\u5728\u533a\u5206\u7532\u4e0e\u4e59\u65f6\u51e0\u4e4e\u6253\u5e73\u3002\u7b2c\u4e09\u679a\u6240\u6709\u5206\u6570\u90fd\u5341\u5206\u63a5\u8fd1\uff0c\u8bf4\u660e\u76f8\u5bf9\u4e8e\u81ea\u8eab\u57fa\u7ebf\uff0c\u7532\u6807\u7b7e\u4e5f\u6ca1\u7a81\u51fa\u591a\u5c11\u3002\u771f\u5b9e\u7ed3\u679c\u7684\u5206\u6570\u70ed\u56fe\u5448\u73b0\u7684\u53ef\u4ee5\u662f\u7ec6\u5316\u5339\u914d\u524d\u7684\u5206\u6570\uff0c\u6700\u540e\u7684\u6807\u7b7e\u53d7\u540e\u7eed\u7ec6\u5316\u5f71\u54cd\u3002\u4e0d\u80fd\u4ece\u989c\u8272\u6700\u6df1\u7684\u4e00\u683c\u5012\u63a8\u51fa\u4e00\u4e2a\u672a\u7ecf\u6838\u5b9e\u7684\u6700\u7ec8\u51b3\u7b56\u8fc7\u7a0b\u3002\u4e0a\u8ff0\u5206\u6570\u4e5f\u6ca1\u6709\u201c\u7532\u7c7b\u578b\u7684\u6982\u7387\u767e\u5206\u4e4b\u516b\u5341\u201d\u8fd9\u6837\u7684\u89e3\u91ca\u3002SingleR \u6ce8\u91ca\u8bca\u65ad\u4e0e\u6807\u7b7e\u4fee\u526a<\/a><\/p>\n

\u7528\u4e00\u5f20\u8868\u8ffd\u67e5\u4fee\u526a\u4e86\u8c01<\/h2>\n

\u5b98\u65b9\u7684\u8bca\u65ad\u4f1a\u8003\u5bdf\u540c\u4e00\u5206\u914d\u6807\u7b7e\u5185\u5404\u4e2a\u7ec6\u80de\u7684\u5dee\u503c\u5206\u5e03\uff0c\u627e\u51fa\u76f8\u5bf9\u504f\u4f4e\u8005\u3002\u5b83\u6709\u4e00\u4e2a\u91cd\u8981\u6761\u4ef6\uff0c\u8fd9\u4e2a\u6807\u7b7e\u4e0b\u591a\u6570\u5206\u914d\u5e94\u5f53\u5927\u4f53\u5408\u7406\u3002\u5982\u679c\u4e00\u6574\u7fa4\u672a\u6536\u5f55\u7684\u7ec6\u80de\u88ab\u4e00\u81f4\u5730\u8bef\u53eb\u6210\u7532\uff0c\u8fd9\u6279\u7ec6\u80de\u5f7c\u6b64\u4e4b\u95f4\u7684\u5dee\u503c\u53ef\u80fd\u8fd8\u5f88\u76f8\u4f3c\uff0c\u9ed8\u8ba4\u4fee\u526a\u4e0d\u4e00\u5b9a\u80fd\u628a\u6574\u7fa4\u627e\u51fa\u6765\u3002\u4fee\u526a\u7ed3\u679c\u9002\u5408\u5f53\u4f5c\u9700\u8981\u590d\u67e5\u7684\u4e00\u7ec4\u7ebf\u7d22\uff0c\u4e0d\u80fd\u53d6\u4ee3\u7559\u51fa\u53c2\u8003\u7c7b\u522b\u3001\u67e5\u770b\u6807\u8bb0\u7b49\u5916\u90e8\u68c0\u67e5\u3002<\/p>\n

\u4e0b\u9762\u5047\u5b9a pred<\/code> \u6765\u81ea\u672c\u6b21 SingleR \u6ce8\u91ca\uff0c\u5143\u6570\u636e\u884c\u540d\u662f\u67e5\u8be2\u6761\u5f62\u7801\uff0c\u542b\u4f9b\u4f53\u5217\u3002\u5148\u6309\u6761\u5f62\u7801\u6821\u51c6\u6b21\u5e8f\u4e4b\u540e\uff0c\u518d\u5206\u6807\u7b7e\u3001\u5206\u4f9b\u4f53\u7edf\u8ba1\u662f\u5426\u88ab\u6807\u4e3a\u5f85\u67e5\u3002\u82e5\u6709\u91cd\u590d\u6761\u5f62\u7801\uff0c\u5e94\u5148\u5c06\u6587\u5e93\u6807\u8bc6\u52a0\u5165\u552f\u4e00\u952e\u3002\u6bd4\u8f83\u4e0d\u540c\u6837\u672c\u65f6\uff0c\u540c\u65f6\u62a5\u544a\u5206\u6bcd\u3002\u4e09\u679a\u7ec6\u80de\u4e2d\u88ab\u4fee\u526a\u4e24\u679a\u548c\u4e09\u5343\u679a\u4e2d\u88ab\u4fee\u526a\u4e24\u679a\uff0c\u6240\u53cd\u6620\u7684\u8986\u76d6\u60c5\u51b5\u663e\u7136\u4e0d\u540c\u3002<\/p>\n

ids <- rownames(pred)\r\nstopifnot(!anyDuplicated(ids), !anyDuplicated(rownames(meta)),\r\n          all(ids %in% rownames(meta)))\r\naudit <- data.frame(label = pred$labels, donor = meta[ids, "donor"],\r\n                    flagged = is.na(pred$pruned.labels))\r\nwith(audit, table(label, flagged))\r\nwith(audit, table(donor, flagged))\r\n<\/code><\/pre>\n
\u62ff\u5230\u5f85\u67e5\u540d\u5355\u540e\uff0c\u5148\u753b\u6bcf\u4e2a\u7ec6\u80de\u7684\u68c0\u51fa\u57fa\u56e0\u6570\u3001\u5206\u5b50\u6570\u548c\u7c97\u8c31\u7cfb\u6807\u8bb0\u3002\u67d0\u4e00\u4f9b\u4f53\u6240\u6709\u7ec6\u80de\u90fd\u7ed9\u51fa\u6a21\u7cca\u6210\u7ee9\uff0c\u4f18\u5148\u67e5\u8fd9\u4e2a\u6587\u5e93\u8d28\u91cf\u3001\u53c2\u8003\u5bf9\u5e94\u7684\u7ec4\u7ec7\u548c\u57fa\u56e0\u6620\u5c04\uff0c\u53ea\u6709\u4e00\u4e2a\u8fb9\u7f18\u5c0f\u7fa4\u6a21\u7cca\uff0c\u5219\u628a\u5b83\u653e\u56de\u539f\u59cb\u8868\u8fbe\u90bb\u57df\uff0c\u770b\u5b83\u50cf\u771f\u6b63\u7684\u8fc7\u6e21\u6001\u3001\u53cc\u7ec6\u80de\uff0c\u8fd8\u662f\u4e00\u79cd\u53c2\u8003\u7f3a\u5c11\u7684\u7c7b\u578b\u3002\u5982\u679c\u660e\u663e\u5c5e\u4e8e\u6dcb\u5df4\u7ec6\u80de\uff0c\u53ea\u662f\u5206\u4e0d\u6e05\u7532\u4e59\u4e24\u4e2a\u8fd1\u4eb2\u4e9a\u578b\uff0c\u53ef\u4ee5\u5148\u505c\u5728\u5171\u540c\u7684\u5927\u7c7b\uff0c\u5e76\u4fdd\u7559\u7ec6\u5206\u672a\u5b9a\u7684\u539f\u56e0\u3002\u4e0d\u8981\u56e0\u4e3a\u60f3\u5728\u56fe\u4f8b\u91cc\u5c11\u4e00\u79cd\u989c\u8272\uff0c\u5c31\u628a\u7f3a\u5931\u503c\u81ea\u52a8\u6539\u56de\u539f\u59cb\u6807\u7b7e\u3002<\/p>\n
\u7edf\u8ba1\u88ab\u4fee\u526a\u7684\u6570\u91cf\u65f6\u518d\u95ee\u4e00\u4e2a\u5206\u6bcd\u95ee\u9898\u3002\u82e5\u67d0\u5c0f\u7fa4\u539f\u6709\u5341\u679a\u7ec6\u80de\uff0c\u6700\u540e\u516d\u679a\u5f85\u5b9a\uff0c\u4f59\u4e0b\u56db\u679a\u90fd\u53eb\u7532\uff0c\u5199\u6210\u201c\u767e\u5206\u4e4b\u767e\u4e3a\u7532\u201d\u4f1a\u63a9\u53bb\u516d\u6210\u672a\u80fd\u786e\u5b9a\u3002\u8868\u91cc\u540c\u65f6\u7ed9\u51fa\u56db\u6bd4\u5341\u548c\u56db\u6bd4\u56db\uff0c\u5206\u522b\u8bf4\u660e\u7532\u5360\u5168\u90e8\u7ec6\u80de\u7684\u6bd4\u4f8b\u53ca\u5176\u5360\u5df2\u5b9a\u540d\u7ec6\u80de\u7684\u6bd4\u4f8b\u3002<\/p>\n
\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u53c2\u8003\u91cc\u62ff\u6389\u4e00\u4e2a\u7fa4\uff0c\u5c11\u4e86\u7684\u7c7b\u522b\u4f1a\u53bb\u54ea\u91cc<\/h2>\n
\u5728 Ubuntu 24.04 \u4e2d\u5b89\u88c5\u4e86 SingleR 2.4.1\uff0c\u7528 R 4.3.3 \u5bf9 SeuratObject \u7684 PBMC \u5c0f\u6837\u672c\u505a\u7559\u51fa\u5b9e\u9a8c\u3002\u628a\u516b\u5341\u679a\u7ec6\u80de\u6309\u539f\u805a\u7c7b\u6807\u7b7e\u5728\u5404\u7fa4\u4ea4\u66ff\u62c6\u6210\u56db\u5341\u4e00\u679a\u53c2\u8003\u548c\u4e09\u5341\u4e5d\u679a\u67e5\u8be2\uff0c\u4fdd\u6301\u67e5\u8be2\u4e0d\u52a8\uff0c\u5148\u7528\u4e09\u4e2a\u53c2\u8003\u6807\u7b7e\u8dd1\u4e00\u6b21\uff0c\u518d\u4ece\u53c2\u8003\u4e2d\u6574\u4f53\u62ff\u6389\u4e8c\u53f7\u7fa4\u3002\u4e09\u7c7b\u53c2\u8003\u90fd\u5728\u65f6\uff0c\u67e5\u8be2\u4e2d\u539f\u5c5e\u4e8c\u53f7\u7684\u4e5d\u679a\u7ec6\u80de\u6709\u4e94\u679a\u4ecd\u88ab\u5206\u4e3a\u4e8c\u53f7\u3002\u53bb\u6389\u4e8c\u53f7\u53c2\u8003\u540e\uff0c\u8fd9\u4e5d\u679a\u6709\u4e09\u679a\u5206\u5230\u96f6\u53f7\u3001\u516d\u679a\u5206\u5230\u4e00\u53f7\uff0c\u9ed8\u8ba4\u4fee\u526a\u53ea\u5c06\u5176\u4e2d\u4e09\u679a\u6807\u4e3a\u7f3a\u5931\uff0c\u53e6\u516d\u679a\u4fdd\u7559\u4e86\u522b\u7684\u7c7b\u522b\u540d\u79f0\u3002<\/p>\n
\u8fd9\u4e2a\u7ed3\u679c\u80fd\u5728\u81ea\u5df1\u7684\u6570\u636e\u4e0a\u590d\u6838\u4e00\u4e2a\u5e38\u89c1\u76f2\u70b9\uff0c\u5373\u4fbf\u4e8b\u5148\u77e5\u9053\u6574\u7c7b\u53c2\u8003\u5df2\u88ab\u5220\u9664\uff0c\u81ea\u52a8\u4fee\u526a\u4e5f\u6ca1\u6709\u628a\u5b83\u4eec\u5168\u90e8\u6807\u51fa\u3002\u6211\u4eec\u4f7f\u7528\u7684\u662f\u540c\u4e00\u4efd\u793a\u4f8b\u6750\u6599\u7684\u539f\u805a\u7c7b\u7f16\u53f7\uff0c\u53ea\u662f\u68c0\u67e5\u53c2\u8003\u7f3a\u5e2d\u600e\u6837\u6539\u53d8\u5206\u914d\uff0c\u4e0d\u80fd\u636e\u6b64\u62a5\u544a\u72ec\u7acb\u4f9b\u4f53\u7684\u7ec6\u80de\u7c7b\u578b\u51c6\u786e\u7387\u3002\u505a\u7ec4\u7ec7\u6ce8\u91ca\u65f6\u5148\u6838\u5b9e\u53c2\u8003\u5e93\u8986\u76d6\uff0c\u518d\u7ed9\u5f97\u5206\u548c\u4fee\u526a\u7ed3\u679c\u5bfb\u627e\u5916\u90e8\u6807\u8bb0\u652f\u6301\u3002\u8fd0\u884c\u6240\u7528 PBMC \u5c0f\u6570\u636e<\/a><\/p>\n
\u505a\u4e00\u6b21\u7559\u51fa\u7c7b\u522b\u7684\u53cd\u5411\u68c0\u67e5<\/h2>\n
\u6709\u5408\u9002\u7684\u5df2\u77e5\u53c2\u8003\u65f6\uff0c\u53ef\u9009\u5176\u4e2d\u4e00\u4e2a\u76f8\u5bf9\u6e05\u695a\u7684\u7c7b\u522b\uff0c\u4ece\u8bad\u7ec3\u53c2\u8003\u4e2d\u6574\u4f53\u62ff\u8d70\uff0c\u7136\u540e\u7528\u8fd9\u7c7b\u5df2\u77e5\u7ec6\u80de\u5f53\u67e5\u8be2\u3002\u89c2\u5bdf\u5b83\u4eec\u88ab\u5206\u5230\u8c01\u3001\u591a\u5c11\u88ab\u4fee\u526a\u3001\u4e0e\u90a3\u4e2a\u8fd1\u4f3c\u7c7b\u522b\u7684\u6807\u8bb0\u5728\u54ea\u4e9b\u4f4d\u7f6e\u51b2\u7a81\u3002\u8fd9\u4e2a\u8bd5\u9a8c\u76f4\u63a5\u6f14\u793a\u4f60\u7684\u53c2\u8003\u4f53\u7cfb\u9047\u5230\u7f3a\u5e2d\u7c7b\u578b\u65f6\u4f1a\u600e\u6837\u51fa\u9519\u3002\u82e5\u540c\u4e00\u4f9b\u4f53\u540c\u65f6\u51fa\u73b0\u5728\u8bad\u7ec3\u4e0e\u67e5\u8be2\u4e2d\uff0c\u6cdb\u5316\u4f1a\u663e\u5f97\u8fc7\u4e8e\u5bb9\u6613\uff0c\u56e0\u6b64\u5c3d\u53ef\u80fd\u6309\u4f9b\u4f53\u72ec\u7acb\u5212\u5206\u3002\u5728\u5df2\u77e5\u6750\u6599\u4e0a\u53d1\u73b0\u7684\u6df7\u6dc6\u5bf9\uff0c\u53ef\u4ee5\u4f18\u5148\u5217\u5165\u65b0\u6837\u672c\u7684\u4eba\u5de5\u590d\u6838\u8868\u3002<\/p>\n
\u5982\u679c\u4e00\u4e2a\u75be\u75c5\u6a21\u578b\u7684\u7a00\u6709\u7fa4\u6070\u597d\u843d\u5728\u8fd9\u4e2a\u6df7\u6dc6\u5bf9\u4e4b\u95f4\uff0c\u7ed9\u5b83\u8865\u505a\u4e00\u7ec4\u4e92\u65a5\u6807\u8bb0\u6bd4\u76f2\u76ee\u8c03\u9ad8\u5168\u4f53\u9608\u503c\u66f4\u6709\u6548\u3002\u5148\u660e\u786e\u9ad8\u5c42\u7ea7\u8eab\u4efd\u662f\u5426\u7a33\u5b9a\uff0c\u518d\u68c0\u67e5\u7ec6\u5206\u7c7b\u5728\u53e6\u4e00\u4efd\u8986\u76d6\u8be5\u7ec4\u7ec7\u7684\u53c2\u8003\u4e2d\u80fd\u5426\u590d\u73b0\uff0c\u540c\u65f6\u8bb0\u5f55\u4e24\u4efd\u53c2\u8003\u7684\u6807\u7b7e\u5b9a\u4e49\u662f\u5426\u771f\u7684\u4e00\u81f4\u3002\u53c2\u8003\u4e00\u7684\u201c\u7956\u7ec6\u80de\u201d\u548c\u53c2\u8003\u4e8c\u7684\u201c\u6d3b\u5316\u7ec6\u80de\u201d\u53ef\u80fd\u63cf\u8ff0\u4e0d\u540c\u7ef4\u5ea6\uff0c\u5355\u7eaf\u7528\u5b57\u9762\u4e00\u81f4\u7387\u8bc4\u4ef7\u8c01\u5bf9\u8c01\u9519\u5bb9\u6613\u8bef\u5bfc\u3002<\/p>\n
\u6700\u540e\u8ba1\u7b97\u7ec6\u80de\u7ec4\u6210\u65f6\uff0c\u4e3a\u6bcf\u4efd\u6837\u672c\u5355\u5217\u5f85\u5b9a\u7ec6\u80de\u6570\uff0c\u5e76\u8bf4\u660e\u5206\u6bcd\u4f7f\u7528\u5168\u90e8\u5408\u683c\u7ec6\u80de\u8fd8\u662f\u4ec5\u4f7f\u7528\u5df2\u6ce8\u91ca\u7ec6\u80de\u3002\u4fdd\u5b58\u539f\u59cb\u6807\u7b7e\u3001\u4fee\u526a\u540e\u6807\u7b7e\u3001\u53c2\u8003\u7248\u672c\u548c\u9700\u8981\u590d\u6838\u7684\u6761\u5f62\u7801\uff0c\u80fd\u8ba9\u540c\u4e8b\u91cd\u590d\u8fd9\u4e00\u5224\u65ad\u3002\u8981\u5728\u56e2\u961f\u4e2d\u7ef4\u62a4\u591a\u4efd\u53c2\u8003\u5e76\u6279\u91cf\u590d\u6838\u65b0\u6837\u672c\u65f6\uff0c\u53ef\u4e86\u89e3\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668<\/a>\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u7528\u4e09\u4e2a\u53ef\u624b\u7b97\u7684\u7ec6\u80de\u89e3\u91ca\u4e0e\u4e2d\u4f4d\u5206\u548c\u6b21\u4f18\u5206\u7684\u5dee\uff0c\u5c55\u793a\u600e\u6837\u5904\u7406\u4fee\u526a\u6807\u7b7e\u3001\u7f3a\u5c11\u7c7b\u522b\u548c\u8fd1\u4eb2\u4e9a\u578b\u3002<\/p>\n","protected":false},"author":1,"featured_media":68217,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49,71],"tags":[700,701,95],"class_list":["post-68218","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","category-single-cell","tag-singler","tag-701","tag-95"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/singler-pruned-labels-median-score-missing-reference-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/68218","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=68218"}],"version-history":[{"count":2,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/68218\/revisions"}],"predecessor-version":[{"id":68506,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/68218\/revisions\/68506"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/68217"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=68218"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=68218"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=68218"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}