{"id":68238,"date":"2026-11-14T20:28:52","date_gmt":"2026-11-14T12:28:52","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=68238"},"modified":"2026-11-14T20:28:52","modified_gmt":"2026-11-14T12:28:52","slug":"cell-ranger-arc-paired-barcodes-umi-cutsite-qc","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/cell-ranger-arc-paired-barcodes-umi-cutsite-qc\/","title":{"rendered":"Cell Ranger ARC \u540c\u4e00\u4e2a\u7ec6\u80de\u6838\u4e3a\u4ec0\u4e48\u6709\u4e24\u4e2a\u6761\u5f62\u7801\uff1f\u628a RNA \u548c ATAC \u914d\u5bf9\u67e5\u6e05\u695a"},"content":{"rendered":"
\n

\u4e00\u4efd 10x Multiome \u6570\u636e\uff0c\u8868\u8fbe\u77e9\u9635\u548c\u5f00\u653e\u67d3\u8272\u8d28\u77e9\u9635\u5404\u6709\u51e0\u5343\u4e2a\u7ec6\u80de\u3002\u7814\u7a76\u751f\u628a\u4e24\u4e2a\u6587\u5e93\u539f\u59cb\u6761\u5f62\u7801\u76f4\u63a5\u53d6\u4ea4\u96c6\uff0c\u7ed3\u679c\u51e0\u4e4e\u6ca1\u6709\u5171\u540c\u7ec6\u80de\uff0c\u6000\u7591\u4e24\u79cd\u5b9e\u9a8c\u6ca1\u6709\u914d\u4e0a\u3002\u8fd9\u91cc\u503c\u5f97\u5148\u533a\u5206\u4e24\u4ef6\u4e8b\u3002\u5b9e\u9a8c\u662f\u5426\u5728\u540c\u4e00\u679a\u7ec6\u80de\u6838\u4e0a\u6d4b\u91cf\uff0c\u4e0e\u4e24\u4e2a\u6587\u5e93\u5199\u5728\u539f\u59cb\u5e8f\u5217\u4e2d\u7684\u6761\u5f62\u7801\u662f\u5426\u9010\u5b57\u76f8\u540c\uff0c\u662f\u4e0d\u540c\u5c42\u9762\u7684\u95ee\u9898\u3002Cell Ranger ARC \u7684\u9010\u6761\u5f62\u7801\u8868\u63d0\u4f9b\u4e86\u5df2\u7ecf\u914d\u597d\u7684\u5bf9\u5e94\u5173\u7cfb\u3002\u987a\u7740\u8fd9\u5f20\u8868\u67e5\u4e0b\u53bb\uff0c\u8fd8\u53ef\u4ee5\u53d1\u73b0\u4e3a\u4ec0\u4e48\u201c\u540c\u4e00\u6838\u7684\u4e24\u4e2a\u8ba1\u6570\u201d\u4e0d\u5b9c\u76f4\u63a5\u76f8\u52a0\u3002<\/p>\n

\u4ece\u5b9e\u9a8c\u8bbe\u8ba1\u627e\u5230\u53ef\u7528\u7684\u5173\u8054\u952e<\/h2>\n

\u5148\u786e\u8ba4\u62ff\u5230\u7684\u662f\u540c\u4e00\u6b21\u9488\u5bf9\u76f8\u540c\u7ec6\u80de\u6838\u7684\u914d\u5bf9\u591a\u7ec4\u5b66\u5b9e\u9a8c\u3002\u5206\u522b\u4ece\u540c\u4e00\u4f9b\u4f53\u505a\u4e00\u4efd\u666e\u901a\u5355\u7ec6\u80de\u8f6c\u5f55\u7ec4\u548c\u4e00\u4efd\u72ec\u7acb\u5355\u7ec6\u80de\u5f00\u653e\u67d3\u8272\u8d28\u5b9e\u9a8c\uff0c\u5c5e\u4e8e\u540c\u4e00\u4e2a\u4eba\uff0c\u5374\u6ca1\u6709\u9010\u6838\u5bf9\u5e94\u5173\u7cfb\u3002\u76f8\u540c\u7684\u7ec4\u7ec7\u6807\u7b7e\u3001\u4e00\u6837\u7684\u4e0a\u673a\u65e5\u671f\u3001\u4e43\u81f3\u5076\u7136\u76f8\u540c\u7684\u4e00\u4e32\u6761\u5f62\u7801\uff0c\u90fd\u4e0d\u80fd\u51ed\u7a7a\u7ed9\u4e24\u79cd\u72ec\u7acb\u5b9e\u9a8c\u914d\u5bf9\u3002\u5148\u627e\u56de\u5b9e\u9a8c\u8bb0\u5f55\u4e2d\u7684\u6355\u83b7\u7f16\u53f7\u3001\u4e24\u4efd\u6587\u5e93\u7684\u914d\u5957\u5173\u7cfb\u548c\u5bf9\u5e94\u7684\u5206\u6790\u8f93\u51fa\u76ee\u5f55\u3002<\/p>\n

ARC \u7684 per_barcode_metrics.csv<\/code> \u91cc\u6709\u7efc\u5408\u7ed3\u679c\u4f7f\u7528\u7684 barcode<\/code>\u3001\u8868\u8fbe\u6587\u5e93\u7684 gex_barcode<\/code> \u548c\u914d\u5bf9\u5f00\u653e\u67d3\u8272\u8d28\u6587\u5e93\u7684 atac_barcode<\/code>\u3002\u5b98\u65b9\u5217\u8bf4\u660e\u4e2d\uff0c\u8868\u8fbe\u6761\u5f62\u7801\u4e0e\u7efc\u5408\u952e\u76f8\u540c\uff0c\u5f00\u653e\u67d3\u8272\u8d28\u6587\u5e93\u8bb0\u5f55\u7684\u662f\u914d\u5bf9\u7684\u90a3\u6761\u5e8f\u5217\u3002\u5728\u540c\u4e00\u4efd ARC \u8f93\u51fa\u91cc\u67e5\u4e00\u4e2a\u7ec6\u80de\u6838\uff0c\u5b9c\u4f7f\u7528\u7efc\u5408\u952e\u8fde\u63a5\u5176\u6ce8\u91ca\uff0c\u518d\u7528\u8868\u5185\u6620\u5c04\u5b9a\u4f4d\u4e24\u79cd\u6587\u5e93\u539f\u59cb\u4fe1\u606f\u3002\u4e0d\u540c\u6355\u83b7\u4e4b\u95f4\u5219\u53e6\u52a0\u6355\u83b7\u7f16\u53f7\u4f5c\u4e3a\u8054\u5408\u952e\uff0c\u907f\u514d\u767d\u540d\u5355\u5e8f\u5217\u91cd\u590d\u4f7f\u7528\u5bfc\u81f4\u9519\u8bef\u5408\u5e76\u300210x \u9010\u6761\u5f62\u7801\u8d28\u63a7\u5b57\u6bb5\u8bf4\u660e<\/a><\/p>\n

\u7528\u4e09\u4e2a\u865a\u6784\u6761\u5f62\u7801\u67e5\u4e00\u6b21\u9519\u914d<\/h2>\n

\u8bbe\u6709\u5982\u4e0b\u6559\u5b66\u6570\u636e\u3002\u4e3a\u65b9\u4fbf\u9605\u8bfb\uff0c\u6761\u5f62\u7801\u7528\u77ed\u540d\u5b57\u4ee3\u66ff\u771f\u5b9e\u5e8f\u5217\u3002\u7532\u6355\u83b7\u4e2d\uff0c\u7efc\u5408\u952e\u7532\u4e00\u7684\u8868\u8fbe\u4fa7\u662f\u7532\u4e00\uff0c\u67d3\u8272\u8d28\u4fa7\u662f\u4e59\u4e5d\u3002\u5982\u679c\u6309\u4e24\u4fa7\u5b57\u7b26\u4e32\u5b8c\u5168\u76f8\u540c\u53bb\u627e\uff0c\u8fd9\u679a\u6709\u660e\u786e\u5bf9\u5e94\u5173\u7cfb\u7684\u7ec6\u80de\u6838\u5c31\u4f1a\u4e22\u5931\u3002\u53e6\u4e00\u4e2a\u9690\u60a3\u662f\u4e59\u6355\u83b7\u4e5f\u6709\u7efc\u5408\u952e\u7532\u4e00\uff0c\u4f46\u5b83\u662f\u53e6\u4e00\u679a\u7ec6\u80de\u6838\uff0c\u7edd\u4e0d\u80fd\u4e0e\u7532\u6355\u83b7\u7684\u7532\u4e00\u5f53\u4f5c\u540c\u4e00\u4e2a\u89c2\u6d4b\u3002<\/p>\n

\n\n\n\n\n\n\n\n
\u6355\u83b7<\/th>\n\u7efc\u5408\u952e\u53ca\u8868\u8fbe\u4fa7<\/th>\n\u5bf9\u5e94 ATAC \u4fa7<\/th>\n\u80fd\u5426\u4e0e\u7b2c\u4e00\u884c\u914d\u6210\u540c\u4e00\u6838<\/th>\n<\/tr>\n<\/thead>\n
\u7532<\/td>\n\u7532\u4e00<\/td>\n\u4e59\u4e5d<\/td>\n\u672c\u884c\u63cf\u8ff0\u540c\u4e00\u6838\u7684\u4e24\u4efd\u6587\u5e93<\/td>\n<\/tr>\n
\u7532<\/td>\n\u7532\u4e8c<\/td>\n\u4e59\u4e03<\/td>\n\u53e6\u4e00\u679a\u6838<\/td>\n<\/tr>\n
\u4e59<\/td>\n\u7532\u4e00<\/td>\n\u4e59\u4e5d<\/td>\n\u6355\u83b7\u4e0d\u540c\uff0c\u4ecd\u662f\u53e6\u4e00\u679a\u6838<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u540c\u4e00\u6838\u8981\u7528\u54ea\u4e00\u5217\u914d\u5bf9\u3002\u540c\u4e00\u6355\u83b7\u5185\u67e5\u5b98\u65b9\u6620\u5c04\uff0c\u8de8\u6355\u83b7\u65f6\u518d\u52a0\u6355\u83b7\u7f16\u53f7\u3002\u7efc\u5408\u952e\uff0c\u4e0e\u8868\u8fbe\u4fa7\u6761\u5f62\u7801\u5bf9\u5e94\uff0c\u5728\u8054\u5408\u77e9\u9635\u4e2d\u5b9a\u4f4d\u540c\u4e00\u679a\u6838\u3002ATAC
\u540c\u4e00\u6355\u83b7\u5185\u67e5\u5b98\u65b9\u6620\u5c04\uff0c\u8de8\u6355\u83b7\u65f6\u518d\u52a0\u6355\u83b7\u7f16\u53f7\u3002<\/figcaption><\/figure>\n

\u4e0b\u9762\u7684\u68c0\u67e5\u4ece\u4e00\u4e2a\u6355\u83b7\u7684 ARC \u8f93\u51fa\u8bfb\u53d6\u5bf9\u5e94\u8868\uff0c\u5148\u4fdd\u7559\u88ab\u5224\u5b9a\u4e0e\u7ec6\u80de\u6838\u5173\u8054\u7684\u6761\u5f62\u7801\uff0c\u518d\u67e5\u770b\u4e24\u79cd\u6d4b\u91cf\u3002\u5bf9\u591a\u4e2a\u6355\u83b7\u5206\u522b\u8bfb\u53d6\u65f6\uff0c\u5c06\u793a\u4f8b\u91cc\u7684\u6355\u83b7\u540d\u6362\u6210\u5b9e\u9645\u7f16\u53f7\uff0c\u62fc\u63a5\u540e\u4ecd\u6309\u4e24\u5217\u4e00\u8d77\u5224\u65ad\u552f\u4e00\u6027\u3002\u5bf9\u4e8e\u6cbf\u7528\u533f\u540d\u7ec6\u80de\u540d\u7684\u4e0b\u6e38\u5bf9\u8c61\uff0c\u6700\u597d\u5efa\u7acb\u5355\u72ec\u6620\u5c04\u8868\uff0c\u4e0d\u8981\u53ea\u51ed\u884c\u6b21\u5e8f\u7ed1\u5b9a\u5143\u6570\u636e\u3002<\/p>\n

import pandas as pd\r\n\r\nqc = pd.read_csv("outs\/per_barcode_metrics.csv")\r\nnuclei = qc.loc[qc["is_cell"].eq(1)].copy()\r\nnuclei["capture"] = "capture_A"\r\nassert not nuclei.duplicated(["capture", "barcode"]).any()\r\nprint(nuclei[["barcode", "gex_barcode", "atac_barcode",\r\n              "gex_umis_count", "atac_fragments"]].head())\r\n<\/code><\/pre>\n
\u6570\u5b57\u90fd\u53eb\u8ba1\u6570\uff0c\u5206\u5b50\u548c\u5207\u70b9\u5404\u6307\u4ec0\u4e48<\/h2>\n
\u8054\u5408\u7279\u5f81\u77e9\u9635\u540c\u4e00\u5217\u5bf9\u5e94\u540c\u4e00\u4e2a\u7efc\u5408\u6761\u5f62\u7801\uff0c\u4e0d\u540c\u7684\u884c\u5206\u6210\u57fa\u56e0\u8868\u8fbe\u548c\u67d3\u8272\u8d28\u5cf0\u4e24\u79cd\u7279\u5f81\u3002\u8868\u8fbe\u884c\u8bb0\u7684\u662f\u5206\u914d\u7ed9\u8fd9\u4e2a\u57fa\u56e0\u548c\u6761\u5f62\u7801\u7684\u53bb\u91cd\u5206\u5b50\u6570\uff0c\u5cf0\u884c\u8bb0\u7684\u662f\u5f52\u4e8e\u8fd9\u4e2a\u5cf0\u548c\u6761\u5f62\u7801\u7684\u5207\u70b9\u6570\u3002\u5f00\u653e\u67d3\u8272\u8d28\u7247\u6bb5\u8fd8\u6709\u4e24\u7aef\uff0c\u5cf0\u5185\u7247\u6bb5\u6570\u3001\u5cf0\u5185\u5207\u70b9\u6570\u548c\u603b\u7247\u6bb5\u6570\u662f\u4e0d\u540c\u7684\u91cf\u3002\u4f8b\u5982\u4e00\u6761\u7247\u6bb5\u4e24\u4e2a\u7aef\u70b9\u90fd\u843d\u5728\u67d0\u5cf0\u5185\uff0c\u5bf9\u8fd9\u4e00\u5cf0\u53ef\u4ee5\u8d21\u732e\u4e24\u4e2a\u5207\u70b9\uff0c\u4f46\u4ecd\u7136\u53ea\u6709\u4e00\u6761\u7247\u6bb5\u3002\u53e6\u4e00\u4e2a\u7247\u6bb5\u82e5\u53ea\u6709\u4e00\u7aef\u5728\u5cf0\u5185\uff0c\u5b83\u53ef\u4ee5\u8d21\u732e\u4e00\u4e2a\u5cf0\u5185\u5207\u70b9\u300210x \u8054\u5408\u77e9\u9635\u7684\u4e24\u79cd\u7279\u5f81\u548c\u8ba1\u6570\u5b9a\u4e49<\/a><\/p>\n
\u82e5\u67d0\u6838\u7684\u4e00\u4e2a\u57fa\u56e0\u8bb0\u5f55\u4e24\u4e2a\u5206\u5b50\uff0c\u90bb\u8fd1\u5cf0\u8bb0\u5f55\u56db\u4e2a\u5207\u70b9\uff0c\u4e0d\u80fd\u628a\u4e24\u8005\u76f8\u52a0\u89e3\u91ca\u4e3a\u8be5\u57fa\u56e0\u6709\u516d\u4efd\u8868\u8fbe\u91cf\u3002\u5b83\u4eec\u6d4b\u91cf\u7684\u662f\u8f6c\u5f55\u548c\u67d3\u8272\u8d28\u53ef\u53ca\u6027\uff0c\u96f6\u503c\u7684\u542b\u4e49\u4e5f\u4e0e\u4e24\u4e2a\u5b9e\u9a8c\u5404\u81ea\u7684\u62bd\u6837\u6df1\u5ea6\u6709\u5173\u3002\u53ef\u4ee5\u5206\u6790\u8fd9\u4e9b\u4fe1\u53f7\u8de8\u7ec6\u80de\u662f\u5426\u5171\u540c\u53d8\u5316\uff0c\u4f46\u4efb\u4f55\u5173\u8054\u90fd\u5e94\u5148\u6838\u5bf9\u771f\u5b9e\u914d\u5bf9\u3001\u8c31\u7cfb\u6784\u6210\u548c\u6d4b\u5e8f\u6df1\u5ea6\u3002\u9760\u8fd1\u57fa\u56e0\u7684\u5cf0\u4e5f\u672a\u5fc5\u76f4\u63a5\u8c03\u63a7\u8be5\u57fa\u56e0\uff0c\u5c24\u5176\u5f53\u591a\u4e2a\u7ec6\u80de\u7c7b\u578b\u540c\u65f6\u53d1\u751f\u6539\u53d8\u65f6\uff0c\u5171\u540c\u7684\u8c31\u7cfb\u5dee\u522b\u8db3\u4ee5\u9020\u6210\u5f88\u5f3a\u7684\u76f8\u5173\u3002<\/p>\n
\u8fd8\u53ef\u62bd\u53d6\u4e00\u4e2a\u771f\u5b9e\u6761\u5f62\u7801\uff0c\u6309\u7279\u5f81\u7c7b\u578b\u5206\u522b\u7ed9\u8be5\u5217\u6c42\u548c\uff0c\u518d\u4e0e\u9010\u6761\u5f62\u7801\u8868\u4e2d\u5b9a\u4e49\u76f8\u7b26\u7684\u5b57\u6bb5\u6838\u5bf9\u3002\u82e5\u6bd4\u8f83\u7684\u662f\u6240\u6709\u6709\u6548\u7247\u6bb5\u4e0e\u5cf0\u5185\u5207\u70b9\uff0c\u9884\u671f\u672c\u6765\u5c31\u4e0d\u76f8\u7b49\u3002\u5148\u8bf4\u660e\u53cc\u65b9\u7edf\u8ba1\u6240\u7528\u5206\u6bcd\u548c\u533a\u57df\u8303\u56f4\uff0c\u624d\u80fd\u5224\u65ad\u5dee\u5f02\u662f\u6b63\u5e38\u53e3\u5f84\u8fd8\u662f\u8bfb\u9519\u4e86\u5217\u3002<\/p>\n
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