{"id":68242,"date":"2026-11-14T20:28:57","date_gmt":"2026-11-14T12:28:57","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=68242"},"modified":"2026-11-14T22:05:37","modified_gmt":"2026-11-14T14:05:37","slug":"signac-merge-atac-common-peaks-fragment-recount","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/signac-merge-atac-common-peaks-fragment-recount\/","title":{"rendered":"\u7528 Signac \u5408\u5e76\u4e24\u4efd ATAC \u6570\u636e\uff0c\u4e3a\u4ec0\u4e48\u540c\u4e00\u4e2a\u5cf0\u4e0d\u80fd\u76f4\u63a5\u628a\u77e9\u9635\u62fc\u8d77\u6765\uff1f"},"content":{"rendered":"
\n

\u7532\u6837\u672c\u7684\u5cf0\u6807\u6210\u4e00\u767e\u5230\u4e8c\u767e\uff0c\u4e59\u6837\u672c\u5728\u9644\u8fd1\u4e5f\u6709\u4e00\u4e2a\u5cf0\uff0c\u53ea\u662f\u8fb9\u754c\u5411\u53f3\u79fb\u4e86\u4e94\u5341\u4e2a\u78b1\u57fa\u3002\u5206\u6790\u4eba\u5458\u60f3\u628a\u4e24\u884c\u5408\u6210\u4e00\u884c\uff0c\u6ca1\u6d4b\u5230\u7684\u4f4d\u7f6e\u586b\u96f6\uff0c\u518d\u6bd4\u8f83\u4e24\u7ec4\u5f00\u653e\u7a0b\u5ea6\u3002\u8fd9\u6837\u505a\u85cf\u7740\u4e00\u4e2a\u96be\u4ee5\u4ece\u56fe\u4e0a\u53d1\u73b0\u7684\u9519\u8bef\u3002\u4e24\u4e2a\u6570\u5b57\u5bf9\u5e94\u7684\u4e0d\u662f\u540c\u4e00\u6bb5\u57fa\u56e0\u7ec4\uff0c\u5373\u4f7f\u7ed9\u4e24\u884c\u8d34\u4e0a\u76f8\u540c\u540d\u79f0\uff0c\u4e5f\u4e0d\u4f1a\u8ba9\u8fc7\u53bb\u7684\u8ba1\u6570\u81ea\u52a8\u8986\u76d6\u65b0\u7684\u8fb9\u754c\u3002Signac \u53ef\u4ee5\u4ece\u5404\u81ea\u7684\u7247\u6bb5\u6587\u4ef6\u91cd\u65b0\u5b9a\u91cf\uff0c\u524d\u63d0\u662f\u5750\u6807\u4f53\u7cfb\u3001\u5cf0\u96c6\u5408\u548c\u7ec6\u80de\u7684\u8eab\u4efd\u90fd\u5bf9\u5f97\u4e0a\u3002<\/p>\n

\u5148\u753b\u4e24\u4e2a\u534a\u91cd\u53e0\u7684\u5cf0\uff0c\u518d\u51b3\u5b9a\u6bd4\u8f83\u4ec0\u4e48<\/h2>\n

\u4e3a\u907f\u514d\u8fb9\u754c\u5199\u6cd5\u6df7\u6dc6\uff0c\u4e0b\u9762\u7684\u6559\u5b66\u7b97\u4f8b\u4f7f\u7528\u5de6\u95ed\u53f3\u5f00\u7684\u5750\u6807\u7ea6\u5b9a\uff0c\u6240\u6709\u7247\u6bb5\u90fd\u653e\u5728\u533a\u95f4\u5185\u90e8\u3002\u7532\u6837\u672c\u539f\u5148\u7684\u5cf0\u662f\u7b2c\u4e00\u767e\u5230\u7b2c\u4e8c\u767e\uff0c\u4e59\u6837\u672c\u7684\u5cf0\u662f\u7b2c\u4e00\u767e\u4e94\u5341\u5230\u7b2c\u4e8c\u767e\u4e94\u5341\uff0c\u4e24\u5cf0\u6709\u4e00\u6bb5\u91cd\u53e0\u3002\u82e5\u79d1\u5b66\u95ee\u9898\u662f\u8fd9\u4e00\u5927\u7247\u5019\u9009\u533a\u57df\u662f\u5426\u6574\u4f53\u66f4\u5f00\u653e\uff0c\u53ef\u4ee5\u9009\u62e9\u5b83\u4eec\u7684\u5e76\u96c6\u4f5c\u4e3a\u5171\u540c\u533a\u57df\u3002\u82e5\u95ee\u9898\u662f\u91cd\u53e0\u6838\u5fc3\u662f\u5426\u6539\u53d8\uff0c\u5c31\u5e94\u4f7f\u7528\u4ea4\u96c6\u3002\u9009\u54ea\u4e00\u4e2a\u76f4\u63a5\u51b3\u5b9a\u5206\u6bcd\u6240\u5bf9\u5e94\u7684\u7a7a\u95f4\u8303\u56f4\uff0c\u5e94\u5728\u8ba1\u7b97\u524d\u8bf4\u660e\u3002<\/p>\n

\u8bbe\u7532\u6765\u6e90\u7684\u7247\u6bb5\u5728\u5de6\u4fa7\u72ec\u6709\u533a\u3001\u5171\u540c\u533a\u3001\u53f3\u4fa7\u72ec\u6709\u533a\u5206\u522b\u6709\u4e09\u3001\u4e8c\u3001\u4e00\u4e2a\u3002\u4e59\u6765\u6e90\u5206\u522b\u6709\u96f6\u3001\u4e8c\u3001\u56db\u4e2a\u3002\u4e0b\u8868\u7279\u610f\u5047\u5b9a\u6bcf\u6761\u7247\u6bb5\u5b8c\u5168\u843d\u5728\u4e00\u4e2a\u5c0f\u533a\u95f4\uff0c\u4fbf\u4e8e\u8bfb\u8005\u624b\u7b97\u3002\u7532\u7684\u539f\u5cf0\u8ba1\u4e94\u4e2a\uff0c\u4e59\u7684\u539f\u5cf0\u8ba1\u516d\u4e2a\u3002\u6309\u5171\u540c\u5e76\u96c6\u91cd\u65b0\u5b9a\u91cf\uff0c\u4e24\u8005\u5176\u5b9e\u90fd\u662f\u516d\u4e2a\uff0c\u6309\u4ea4\u96c6\u91cd\u65b0\u5b9a\u91cf\uff0c\u5219\u90fd\u662f\u4e24\u4e2a\u3002<\/p>\n

\n\n\n\n\n\n\n\n
\u5728\u54ea\u4e00\u6bb5\u6bd4\u8f83<\/th>\n\u7532\u6837\u672c<\/th>\n\u4e59\u6837\u672c<\/th>\n\u5b83\u4eec\u662f\u5426\u6307\u540c\u4e00\u6bb5\u5750\u6807<\/th>\n<\/tr>\n<\/thead>\n
\u5404\u81ea\u539f\u6765\u7684\u5cf0<\/td>\n3 + 2 = 5<\/td>\n2 + 4 = 6<\/td>\n\u5de6\u53f3\u8fb9\u754c\u4e0d\u540c<\/td>\n<\/tr>\n
\u4e24\u5cf0\u5e76\u96c6<\/td>\n3 + 2 + 1 = 6<\/td>\n0 + 2 + 4 = 6<\/td>\n\u540c\u4e00\u5e76\u96c6<\/td>\n<\/tr>\n
\u4e24\u5cf0\u4ea4\u96c6<\/td>\n2<\/td>\n2<\/td>\n\u540c\u4e00\u4ea4\u96c6<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u4e24\u4e2a\u6837\u672c\u539f\u6765\u662f\u4e94\u548c\u516d\uff0c\u91cd\u6570\u5462\u3002\u6559\u5b66\u7b97\u4f8b\uff5c\u6bd4\u8f83\u533a\u57df\u4ece\u5404\u81ea\u539f\u5cf0\u6539\u4e3a\u7edf\u4e00\u7684\u5e76\u96c6\u6216\u4ea4\u96c6\u3002\u539f\u5cf0\uff0c\u7532\u4e3a\u4e94\uff0c\u4e59\u4e3a\u516d\uff0c\u6837\u672c\u5404\u81ea\u4f7f\u7528\u7684\u8fb9\u754c\u4e0d\u540c\u3002\u5e76\u96c6\uff0c\u7532\u4e3a\u516d\uff0c\u4e59\u4e3a\u516d\uff0c\u540c\u4e00\u533a\u57df\u5185\u7247\u6bb5\u603b\u6570\u76f8\u7b49\u3002\u4ea4\u96c6\uff0c\u7532\u4e3a\u4e8c\uff0c\u4e59\u4e3a\u4e8c\uff0c\u8fd8\u9700\u68c0\u67e5\u5e76\u96c6\u5de6\u53f3\u4e24\u4fa7\u4fe1\u53f7\u662f\u5426\u91cd\u65b0\u5206\u5e03\u3002\"
\u6559\u5b66\u7b97\u4f8b\uff5c\u6bd4\u8f83\u533a\u57df\u4ece\u5404\u81ea\u539f\u5cf0\u6539\u4e3a\u7edf\u4e00\u7684\u5e76\u96c6\u6216\u4ea4\u96c6\u3002<\/figcaption><\/figure>\n

\u8fd9\u4e2a\u4f8b\u5b50\u8fd8\u4fdd\u7559\u4e86\u4e00\u4e2a\u771f\u5b9e\u7684\u751f\u7269\u5b66\u53ef\u80fd\u3002\u5c3d\u7ba1\u4e24\u4e2a\u6837\u672c\u7684\u5e76\u96c6\u603b\u91cf\u76f8\u540c\uff0c\u7532\u7684\u5de6\u4fa7\u66f4\u5f3a\uff0c\u4e59\u7684\u53f3\u4fa7\u66f4\u5f3a\u3002\u82e5\u53ea\u6c47\u62a5\u5408\u5e76\u540e\u7684\u603b\u6570\uff0c\u5c31\u4f1a\u9519\u8fc7\u5c40\u90e8\u4f4d\u7f6e\u53d8\u5316\u3002\u5bf9\u4e8e\u7279\u522b\u5bbd\u3001\u5305\u542b\u591a\u4e2a\u5c40\u90e8\u9ad8\u70b9\u7684\u533a\u57df\uff0c\u53ef\u4ee5\u67e5\u770b\u5206\u4f9b\u4f53\u7684\u8986\u76d6\u66f2\u7ebf\uff0c\u6309\u6709\u4f9d\u636e\u7684\u7a84\u533a\u95f4\u53e6\u4f5c\u6bd4\u8f83\u3002\u591a\u4e2a\u4e92\u76f8\u642d\u8fb9\u7684\u5cf0\u8fd8\u53ef\u80fd\u88ab\u4e32\u6210\u4e00\u4e2a\u5f88\u957f\u7684\u5e76\u96c6\uff0c\u5408\u5e76\u540e\u5148\u67e5\u5bbd\u5ea6\u5206\u5e03\u3001\u5f02\u5e38\u5bbd\u533a\u57df\u53ca\u5176\u9644\u8fd1\u7684\u9ad8\u70b9\u3002Signac \u4f5c\u8005\u7684\u591a\u6837\u672c\u5408\u5e76\u6559\u7a0b<\/a><\/p>\n

\u4ece\u7247\u6bb5\u6587\u4ef6\u91cd\u6570\uff0c\u800c\u4e0d\u662f\u4ece\u4e24\u4e2a\u6210\u54c1\u77e9\u9635\u731c<\/h2>\n

\u7528\u5171\u540c\u7684\u533a\u57df\u96c6\u5728\u6bcf\u4e2a\u6837\u672c\u7684\u7247\u6bb5\u6587\u4ef6\u4e0a\u8ba1\u6570\uff0c\u53ef\u83b7\u5f97\u5b9a\u4e49\u4e00\u81f4\u7684\u884c\u3002\u53ea\u62e5\u6709\u539f\u5cf0\u77e9\u9635\u65f6\uff0c\u7532\u5cf0\u7684\u4e94\u4e2a\u8ba1\u6570\u7a76\u7adf\u6709\u51e0\u4e2a\u843d\u5728\u4ea4\u96c6\u3001\u51e0\u4e2a\u843d\u5728\u5de6\u4fa7\uff0c\u4ece\u4e00\u4e2a\u603b\u6570\u65e0\u6cd5\u53cd\u63a8\u3002\u7269\u7406\u4e0a\u672a\u89c2\u5bdf\u5230\u7684\u533a\u6bb5\u4e5f\u4e0d\u80fd\u4e00\u5f8b\u89e3\u91ca\u6210\u96f6\u3002\u5c24\u5176\u5f53\u4e24\u4e2a\u6570\u636e\u6765\u81ea\u4e0d\u540c\u53c2\u8003\u7248\u672c\u3001\u4e0d\u540c\u67d3\u8272\u4f53\u547d\u540d\uff0c\u6216\u6709\u4e00\u65b9\u7f3a\u67d0\u6761\u67d3\u8272\u4f53\u7684\u7247\u6bb5\u65f6\uff0c\u5f3a\u884c\u8865\u96f6\u4f1a\u5236\u9020\u751f\u7269\u5b66\u5dee\u5f02\u3002\u5148\u786e\u8ba4\u4e8c\u8005\u4f7f\u7528\u540c\u4e00\u53c2\u8003\u5750\u6807\uff0c\u68c0\u67e5\u4e3b\u8981\u67d3\u8272\u4f53\u548c\u76ee\u6807\u533a\u95f4\u80fd\u5426\u771f\u5b9e\u67e5\u8be2\u3002<\/p>\n

\u7247\u6bb5\u6587\u4ef6\u5e94\u91c7\u7528\u53ef\u6309\u533a\u95f4\u67e5\u8be2\u7684\u683c\u5f0f\uff0c\u5e76\u6709\u540c\u76ee\u5f55\u5bf9\u5e94\u7684\u7d22\u5f15\u3002\u6a21\u677f\u91cc\u7684 CreateFragmentObject<\/code> \u8fd8\u53ef\u6838\u5bf9\u6307\u5b9a\u7ec6\u80de\u662f\u5426\u5728\u6587\u4ef6\u4e2d\u5b58\u5728\u3002\u5982\u679c\u4e3a\u5408\u5e76\u5bf9\u8c61\u7ed9\u7ec6\u80de\u52a0\u4e0a\u6837\u672c\u524d\u7f00\uff0c\u6784\u9020\u6620\u5c04\u65f6\uff0c\u5411\u91cf\u7684\u540d\u5b57\u662f\u5bf9\u8c61\u91cc\u7684\u7ec6\u80de\u540d\uff0c\u5411\u91cf\u7684\u503c\u624d\u662f\u6587\u4ef6\u7b2c\u56db\u5217\u5b58\u653e\u7684\u539f\u59cb\u6761\u5f62\u7801\u3002\u628a\u4e24\u8005\u5012\u7f6e\uff0c\u5373\u4f7f\u6240\u6709\u5b57\u7b26\u4e32\u90fd\u773c\u719f\u4e5f\u53ef\u80fd\u67e5\u4e0d\u5230\u5bf9\u5e94\u7ec6\u80de\u3002\u4e24\u4e2a\u6587\u5e93\u6070\u597d\u6709\u76f8\u540c\u539f\u59cb\u6761\u5f62\u7801\u65f6\uff0c\u66f4\u9700\u8981\u5404\u81ea\u5e26\u6837\u672c\u524d\u7f00\u4fdd\u7559\u8eab\u4efd\u3002\u7247\u6bb5\u5bf9\u8c61\u53ca\u6761\u5f62\u7801\u6620\u5c04<\/a><\/p>\n

\u4e0b\u9762\u793a\u610f\u4e00\u4e2a\u6837\u672c\u7684\u8ba1\u6570\u3002\u5047\u8bbe\u5df2\u6709\u6309\u540c\u4e00\u7ec4\u88c5\u7248\u672c\u5efa\u7acb\u7684\u57fa\u56e0\u7ec4\u533a\u95f4\u5bf9\u8c61\uff0c\u5e76\u4e14\u539f\u6761\u5f62\u7801\u5411\u91cf\u6765\u81ea\u8be5\u6355\u83b7\u901a\u8fc7\u8d28\u63a7\u7684\u7ec6\u80de\u3002\u5bf9\u7b2c\u4e8c\u4e2a\u6837\u672c\u7528\u81ea\u5df1\u7684\u7247\u6bb5\u6587\u4ef6\u548c\u7ec6\u80de\u6807\u8bc6\u6267\u884c\u540c\u6837\u8fc7\u7a0b\uff0c\u6bd4\u8f83\u8fd4\u56de\u7684\u884c\u540d\u548c\u987a\u5e8f\uff0c\u68c0\u67e5\u4e00\u81f4\u540e\u518d\u62fc\u5217\u3002\u5355\u72ec\u8bb0\u4e0b\u7a7a\u533a\u95f4\u548c\u7f3a\u5c11\u7684\u67d3\u8272\u4f53\uff0c\u4e0d\u8981\u4ec5\u4e3a\u7ed5\u8fc7\u7ef4\u5ea6\u4e0d\u540c\u7684\u62a5\u9519\u800c\u8865\u9f50\u6570\u5b57\u3002FeatureMatrix \u7684\u8f93\u5165\u548c\u8fd4\u56de\u503c<\/a><\/p>\n

library(Signac)\r\nmap <- setNames(raw_barcodes, paste0("A_", raw_barcodes))\r\nfr <- CreateFragmentObject(path = "A\/fragments.tsv.gz", cells = map)\r\nmat_a <- FeatureMatrix(fragments = fr, features = common_ranges,\r\n                       cells = names(map))\r\nstopifnot(!anyDuplicated(rownames(mat_a)), !anyDuplicated(colnames(mat_a)))\r\n<\/code><\/pre>\n
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\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u5171\u540c\u5cf0\u603b\u6570\u76f8\u7b49\uff0c\u53f3\u4fa7\u5c40\u90e8\u8ba1\u6570\u5374\u662f\u56db\u6bd4\u4e00<\/h2>\n
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