{"id":68650,"date":"2026-11-14T20:57:30","date_gmt":"2026-11-14T12:57:30","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=68650"},"modified":"2026-11-14T20:57:30","modified_gmt":"2026-11-14T12:57:30","slug":"space-ranger-spatial-coordinates-scale-crop-landmarks","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/space-ranger-spatial-coordinates-scale-crop-landmarks\/","title":{"rendered":"Space Ranger \u7684\u70b9\u4e3a\u4ec0\u4e48\u843d\u5728\u7ec4\u7ec7\u5916\uff1f\u7528\u4e09\u4e2a\u5730\u6807\u67e5\u5750\u6807\u3001\u7f29\u653e\u548c\u88c1\u56fe"},"content":{"rendered":"
\n

\u5728\u7a7a\u95f4\u8f6c\u5f55\u7ec4\u7ed3\u679c\u91cc\uff0c\u5c06\u8868\u8fbe\u6700\u9ad8\u7684\u70b9\u53e0\u5230\u7ec4\u7ec7\u7167\u7247\u4e0a\uff0c\u672c\u8be5\u4f4d\u4e8e\u4e0a\u76ae\u7684\u4e00\u6392\u70b9\u5374\u843d\u8fdb\u4e86\u7a7a\u767d\u3002\u6709\u4eba\u60f3\u5148\u8c03\u70b9\u7684\u5927\u5c0f\uff0c\u6709\u4eba\u6000\u7591\u90a3\u5757\u7ec4\u7ec7\u6355\u83b7\u5931\u8d25\u3002\u66f4\u5feb\u7684\u529e\u6cd5\u662f\u6682\u65f6\u4e0d\u770b\u8868\u8fbe\uff0c\u53ea\u6311\u4e09\u4e2a\u4e0d\u5728\u540c\u4e00\u76f4\u7ebf\u4e0a\u7684\u5730\u6807\uff0c\u628a\u5b83\u4eec\u4ece\u8f6f\u4ef6\u63d0\u4f9b\u7684\u539f\u56fe\u4f4d\u7f6e\u9010\u4e00\u6362\u7b97\u5230\u51c6\u5907\u7ed8\u5236\u7684\u5e95\u56fe\u3002\u5982\u679c\u8fde\u4e09\u4e2a\u4f4d\u7f6e\u90fd\u5bf9\u4e0d\u4e0a\uff0c\u57fa\u56e0\u8868\u8fbe\u56fe\u4e0a\u7684\u504f\u79fb\u5c31\u4e0d\u8be5\u5148\u7528\u751f\u7269\u5b66\u89e3\u91ca\u3002<\/p>\n

\u5f04\u6e05\u6b63\u5728\u7528\u7684\u5750\u6807\u5c5e\u4e8e\u54ea\u5f20\u56fe<\/h2>\n

Space Ranger \u7684\u7a7a\u95f4\u8f93\u51fa\u533a\u5206\u9635\u5217\u4e2d\u7684\u884c\u5217\u548c\u663e\u5fae\u56fe\u50cf\u4e0a\u7684\u50cf\u7d20\u4f4d\u7f6e\u3002\u9635\u5217\u884c\u5217\u8868\u793a\u70b9\u5728\u82af\u7247\u5e03\u5c40\u4e2d\u7684\u7f16\u53f7\uff0c\u5168\u5206\u8fa8\u7387\u7684\u50cf\u7d20\u884c\u3001\u5217\u5219\u63cf\u8ff0\u5b83\u5728\u5bf9\u5e94\u539f\u56fe\u4e2d\u7684\u4e2d\u5fc3\u3002\u753b\u70b9\u65f6\u4e00\u822c\u7531\u5217\u7ed9\u51fa\u6a2a\u5750\u6807\u3001\u884c\u7ed9\u51fa\u7eb5\u5750\u6807\u3002\u5982\u679c\u628a\u7b2c\u4e00\u5217\u8bfb\u4f5c\u6a2a\u8f74\u53ea\u662f\u56e0\u4e3a\u82f1\u6587\u540d\u91cc\u6709\u4e00\u4e2a\u201c\u884c\u201d\uff0c\u5f97\u5230\u7684\u56fe\u53ef\u80fd\u6cbf\u5bf9\u89d2\u7ebf\u7ffb\u8fc7\u53bb\u3002\u53e6\u4e00\u4e2a\u7ec6\u8282\u662f\u5e38\u89c1\u56fe\u50cf\u5750\u6807\u7684\u7eb5\u5411\u5411\u4e0b\uff0c\u666e\u901a\u6563\u70b9\u56fe\u7eb5\u8f74\u5411\u4e0a\u3002\u5728\u7ed8\u56fe\u5e93\u4e2d\u5e94\u8ba9\u70b9\u548c\u5e95\u56fe\u4f7f\u7528\u540c\u4e00\u65b9\u5411\u3002Space Ranger \u7a7a\u95f4\u6587\u4ef6\u4e0e\u5750\u6807\u5b9a\u4e49<\/a><\/p>\n

\u4ece\u539f\u56fe\u6362\u5230\u8f6f\u4ef6\u751f\u6210\u7684\u9ad8\u5206\u8fa8\u7387\u6216\u4f4e\u5206\u8fa8\u7387\u5c55\u793a\u56fe\u65f6\uff0c\u8981\u53d6\u5bf9\u5e94\u7684\u7f29\u653e\u56e0\u5b50\u3002\u540c\u4e00\u5f20\u5207\u7247\u7684\u4e24\u79cd\u5c55\u793a\u56fe\u6709\u4e0d\u540c\u7684\u56e0\u5b50\uff0c\u4e0d\u80fd\u770b\u5230\u6587\u4ef6\u540d\u90fd\u542b\u7ec4\u7ec7\u56fe\u50cf\u5c31\u4e92\u6362\u3002\u4e5f\u4e0d\u8981\u62ff\u4e00\u4e2a\u6837\u672c\u7684\u56e0\u5b50\u6279\u91cf\u7528\u4e8e\u5176\u4ed6\u6837\u672c\u3002\u6574\u7406\u6570\u636e\u65f6\uff0c\u628a\u5207\u7247\u7f16\u53f7\u3001\u5750\u6807\u6587\u4ef6\u3001\u7f29\u653e\u6587\u4ef6\u3001\u5b9e\u9645\u4f7f\u7528\u7684\u7ec4\u7ec7\u56fe\u548c\u53c2\u8003\u8f93\u51fa\u76ee\u5f55\u653e\u5728\u540c\u4e00\u4e2a\u6e05\u5355\u91cc\u3002\u5bf9\u4e8e\u9ad8\u5bc6\u5ea6\u6570\u636e\uff0c\u786e\u8ba4\u6240\u9009\u6587\u4ef6\u5c5e\u4e8e\u54ea\u4e2a\u5206\u7bb1\u5c42\u7ea7\uff0c\u90e8\u5206\u4f4d\u7f6e\u8868\u4ee5\u5217\u5f0f\u6587\u4ef6\u4fdd\u5b58\uff0c\u666e\u901a\u70b9\u4f4d\u7684\u793a\u4f8b\u8bfb\u6cd5\u672a\u5fc5\u76f4\u63a5\u9002\u7528\u3002<\/p>\n

\u624b\u7b97\u4e00\u4e2a\u70b9\u4ece\u539f\u56fe\u5230\u88c1\u526a\u56fe\u7684\u5750\u6807<\/h2>\n

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\n\n\n\n\n\n\n\n
\u5e95\u56fe<\/th>\n\u6a2a\u5750\u6807<\/th>\n\u7eb5\u5750\u6807<\/th>\n<\/tr>\n<\/thead>\n
\u5bf9\u5e94\u7684\u5168\u5206\u8fa8\u7387\u539f\u56fe<\/td>\n2000<\/td>\n1200<\/td>\n<\/tr>\n
\u7f29\u5c0f\u5230\u539f\u6765\u7684 0.2 \u500d<\/td>\n400<\/td>\n240<\/td>\n<\/tr>\n
\u5728\u7f29\u5c0f\u56fe\u4e0a\u518d\u4ece\u5de6\u88c1 100\u3001\u4ece\u4e0a\u88c1 40<\/td>\n300<\/td>\n200<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u540c\u4e00\u4e2a\u70b9\u600e\u6837\u843d\u56de\u88c1\u526a\u56fe\u3002\u6559\u5b66\u7b97\u4f8b\uff5c\u50cf\u7d20\u884c\u7ed9\u7eb5\u5750\u6807\uff0c\u5217\u7ed9\u6a2a\u5750\u6807\u3002\u539f\u56fe\uff0c\u6a2a
\u6559\u5b66\u7b97\u4f8b\uff5c\u50cf\u7d20\u884c\u7ed9\u7eb5\u5750\u6807\uff0c\u5217\u7ed9\u6a2a\u5750\u6807\u3002<\/figcaption><\/figure>\n

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\u4ee5\u4e0b\u793a\u610f\u9488\u5bf9\u542b\u5217\u540d\u7684\u4f4d\u7f6e\u8868\u548c\u540c\u6279\u9ad8\u5206\u8fa8\u7387\u7ec4\u7ec7\u56fe\u3002\u82e5\u5bf9\u8c61\u5df2\u7ecf\u63d0\u524d\u6362\u7b97\u8fc7\u5750\u6807\uff0c\u518d\u4e58\u4e00\u6b21\u56e0\u5b50\u4f1a\u628a\u8868\u8fbe\u533a\u57df\u6324\u5411\u539f\u70b9\uff0c\u5fc5\u987b\u5148\u67e5\u6570\u503c\u6765\u6e90\u3002\u793a\u4f8b\u6309\u539f\u59cb\u6761\u5f62\u7801\u4fdd\u7559\u7d22\u5f15\uff0c\u65b9\u4fbf\u548c\u8868\u8fbe\u77e9\u9635\u7684\u5217\u540d\u7cbe\u786e\u5bf9\u9f50\u3002<\/p>\n

import json\r\nimport pandas as pd\r\n\r\npos = pd.read_csv("spatial\/tissue_positions.csv").set_index("barcode")\r\nwith open("spatial\/scalefactors_json.json", encoding="utf-8") as src:\r\n    scale = json.load(src)["tissue_hires_scalef"]\r\npos["x_hires"] = pos["pxl_col_in_fullres"] * scale\r\npos["y_hires"] = pos["pxl_row_in_fullres"] * scale\r\nprint(pos[["in_tissue", "x_hires", "y_hires"]].head())\r\n<\/code><\/pre>\n
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\u6211\u4eec\u4e0b\u8f7d\u4e86\u5b98\u65b9\u4eba\u6dcb\u5df4\u7ed3\u6837\u672c\u7684\u7a7a\u95f4\u8f93\u51fa\uff0c\u7528 Python \u548c Pillow 13.0.0 \u8bfb\u53d6\u9ad8\u5206\u8fa8\u7387\u7ec4\u7ec7\u56fe\u5e76\u91cd\u7b97\u4f4d\u7f6e\u3002\u56fe\u7247\u5bbd 1,921\u3001\u9ad8 2,000 \u50cf\u7d20\uff0c\u7f29\u653e\u56e0\u5b50\u4e3a 0.17011142\u3002\u4f4d\u7f6e\u8868\u6709 4,992 \u884c\uff0c\u7ec4\u7ec7\u5185\u6807\u8bb0\u4e3a\u4e00\u7684\u6709 4,035 \u884c\u3002\u8fd9\u4efd\u65e7\u7248\u8f93\u51fa\u7684\u6587\u4ef6\u540d\u5e26\u6709 list\uff0c\u800c\u4e14\u6ca1\u6709\u8868\u5934\u3002\u5982\u679c\u8ba9\u8bfb\u53d6\u51fd\u6570\u81ea\u52a8\u628a\u9996\u884c\u5f53\u4f5c\u5b57\u6bb5\u540d\uff0c\u4f1a\u9759\u9ed8\u4e22\u6389\u7b2c\u4e00\u4e2a\u6761\u5f62\u7801\u3002\u5148\u6309\u516d\u4e2a\u5b57\u6bb5\u663e\u5f0f\u547d\u540d\uff0c\u518d\u4e0e\u8f6f\u4ef6\u7f51\u9875\u62a5\u544a\u7684\u7ec4\u7ec7\u5185\u70b9\u6570\u4ea4\u53c9\u6838\u5bf9\uff0c\u53ef\u4ee5\u533a\u5206\u8bfb\u8868\u95ee\u9898\u548c\u7ec4\u7ec7\u8bc6\u522b\u95ee\u9898\u3002\u8be5\u6837\u672c\u7684\u5b98\u65b9\u8fd0\u884c\u62a5\u544a<\/a><\/p>\n
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\n\n\n\n\n\n\n\n
\u4f4d\u7f6e\u8868\u6761\u5f62\u7801<\/th>\n\u5c55\u793a\u56fe\u6a2a\u5750\u6807<\/th>\n\u5c55\u793a\u56fe\u7eb5\u5750\u6807<\/th>\n<\/tr>\n<\/thead>\n
CTAACAGCACAATAAC-1<\/td>\n237.48<\/td>\n1,414.82<\/td>\n<\/tr>\n
TGTTGATCACTGTTTA-1<\/td>\n1,628.14<\/td>\n209.24<\/td>\n<\/tr>\n
AGGAGCGTTTATTATC-1<\/td>\n1,573.19<\/td>\n1,737.52<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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