{"id":68830,"date":"2026-11-14T21:03:27","date_gmt":"2026-11-14T13:03:27","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=68830"},"modified":"2026-11-14T21:03:27","modified_gmt":"2026-11-14T13:03:27","slug":"xenium-ranger-segmentation-transcript-assignment-audit","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/xenium-ranger-segmentation-transcript-assignment-audit\/","title":{"rendered":"Xenium \u6362\u4e86\u7ec6\u80de\u5206\u5272\u540e\uff0c\u4e00\u4e2a\u57fa\u56e0\u4e3a\u4ec0\u4e48\u4ece\u4e0a\u76ae\u8dd1\u5230\u4e86\u514d\u75ab\u7ec6\u80de\uff1f"},"content":{"rendered":"
\n

\u5bf9\u540c\u4e00\u5757\u7ec4\u7ec7\u91cd\u590d\u4f7f\u7528\u4e24\u5957\u7ec6\u80de\u8fb9\u754c\uff0c\u57fa\u56e0\u7532\u5728\u514d\u75ab\u7ec6\u80de\u4e2d\u7a81\u7136\u53d8\u591a\u4e86\u3002\u6b64\u65f6\u6709\u4e00\u4e2a\u6bd4\u91cd\u65b0\u805a\u7c7b\u66f4\u5feb\u7684\u529e\u6cd5\uff0c\u6311\u51fa\u4f4d\u7f6e\u6ca1\u53d8\u7684\u90a3\u51e0\u4e2a\u8f6c\u5f55\u672c\uff0c\u770b\u5b83\u4eec\u5728\u4e24\u5957\u8fb9\u754c\u4e0b\u5206\u522b\u5f52\u7ed9\u8c01\u3002Xenium Ranger \u53ef\u4ee5\u5728\u540c\u4e00\u5b9e\u9a8c\u7ed3\u679c\u4e0a\u5bfc\u5165\u7ec6\u80de\u6216\u7ec6\u80de\u6838\u5206\u5272\u5e76\u91cd\u65b0\u6c47\u603b\u3002\u5206\u5b50\u4f4d\u7f6e\u3001\u5206\u5272\u8fb9\u754c\u548c\u7ec6\u80de\u7c7b\u578b\u6807\u7b7e\u5206\u522b\u6765\u81ea\u4e0d\u540c\u6b65\u9aa4\uff0c\u5148\u5f04\u6e05\u6539\u4e86\u54ea\u4e00\u5c42\uff0c\u624d\u8c08\u5f97\u4e0a\u8fd9\u4e2a\u57fa\u56e0\u662f\u5426\u771f\u6709\u8de8\u8c31\u7cfb\u8868\u8fbe\u3002<\/p>\n

\u5148\u56fa\u5b9a\u5206\u5b50\u6e05\u5355\uff0c\u518d\u8bf4\u660e\u6362\u7684\u662f\u54ea\u79cd\u8fb9\u754c<\/h2>\n

\u4fdd\u7559\u539f\u59cb\u5206\u6790\u5305\u3001\u56fe\u50cf\u5750\u6807\u6846\u67b6\u548c\u672c\u6b21\u8981\u4f7f\u7528\u7684\u5206\u5b50\u96c6\u5408\u3002\u6bd4\u8f83\u65f6\u8981\u8ba9\u4e24\u7248\u4f7f\u7528\u76f8\u540c\u7684\u57fa\u56e0\u9762\u677f\u3001\u8d28\u91cf\u9608\u503c\u548c\u8f6c\u5f55\u672c\u6807\u8bc6\u3002\u5b98\u65b9\u7b97\u6cd5\u8bf4\u660e\u4e2d\uff0c\u6784\u5efa\u7ec6\u80de\u7279\u5f81\u77e9\u9635\u65f6\u91c7\u7528\u8d28\u91cf\u503c\u8fbe\u5230\u4e8c\u5341\u7684\u8f6c\u5f55\u672c\u3002\u5728\u6f14\u793a\u5355\u4e2a\u57fa\u56e0\u7684\u6539\u53d8\u65f6\uff0c\u8fd8\u5e94\u5355\u72ec\u8bc6\u522b\u9634\u6027\u5bf9\u7167\u63a2\u9488\u6216\u5176\u4ed6\u63a7\u5236\u7279\u5f81\u3002\u82e5\u4e00\u7248\u7528\u5168\u90e8\u68c0\u6d4b\u70b9\u3001\u53e6\u4e00\u7248\u53ea\u753b\u901a\u8fc7\u9608\u503c\u7684\u5206\u5b50\uff0c\u5c31\u540c\u65f6\u6539\u53d8\u4e86\u5206\u5b50\u6e05\u5355\u548c\u8fb9\u754c\uff0c\u540e\u7eed\u5dee\u503c\u65e0\u6cd5\u53ea\u5f52\u56e0\u4e8e\u5206\u5272\u3002Xenium Ranger \u7b97\u6cd5\u4e0e\u5206\u5b50\u8ba1\u6570\u8bf4\u660e<\/a><\/p>\n

\u5f04\u6e05\u8f93\u5165\u6765\u81ea\u6838\u3001\u6574\u7ec6\u80de\u63a9\u819c\u3001\u7ec6\u80de\u591a\u8fb9\u5f62\uff0c\u8fd8\u662f\u5df2\u6709\u7684\u9010\u5206\u5b50\u5f52\u5c5e\u8868\u3002\u53ea\u63d0\u4f9b\u6838\u65f6\uff0c\u540e\u7eed\u7ec6\u80de\u8fb9\u754c\u53ef\u4ee5\u7531\u6838\u5411\u5916\u6269\u5c55\u5f97\u5230\uff0c\u63d0\u4f9b\u6574\u7ec6\u80de\u7ed3\u679c\u5219\u662f\u5728\u8ba8\u8bba\u53e6\u4e00\u79cd\u8fb9\u754c\u5b9a\u4e49\u3002\u5bfc\u5165\u5df2\u6709\u9010\u5206\u5b50\u5f52\u5c5e\u65f6\uff0c\u8fd8\u8981\u786e\u8ba4\u8f6c\u5f55\u672c\u6807\u8bc6\u4e0e\u539f\u5b9e\u9a8c\u51c6\u786e\u5bf9\u5e94\u3002\u4e09\u79cd\u8def\u7ebf\u90fd\u80fd\u5f97\u5230\u4e00\u4e2a\u6309\u7ec6\u80de\u6c47\u603b\u7684\u8868\uff0c\u5dee\u522b\u5728\u4e8e\u201c\u8fd9\u679a\u5206\u5b50\u4e3a\u4ec0\u4e48\u7b97\u8fdb\u6765\u201d\u7684\u8bc1\u636e\u6765\u6e90\u3002\u5c06\u8f93\u5165\u7c7b\u578b\u548c\u8fd0\u884c\u53c2\u6570\u5199\u8fdb\u56fe\u6ce8\uff0c\u6bd4\u7b3c\u7edf\u8bf4\u6362\u4e86\u65b0\u7248\u5206\u5272\u66f4\u6709\u610f\u4e49\u3002\u5bfc\u5165\u6838\u3001\u7ec6\u80de\u8fb9\u754c\u6216\u8f6c\u5f55\u672c\u5206\u914d\u7684\u5b98\u65b9\u6d41\u7a0b<\/a><\/p>\n

\u505a\u4e00\u5f20\u5206\u5b50\u53bb\u5411\u8868\uff0c\u4e0d\u53ea\u6bd4\u8f83\u4e24\u4e2a\u603b\u6570<\/h2>\n

\u5047\u5b9a\u67d0\u89c6\u91ce\u91cc\u57fa\u56e0\u7532\u6070\u6709\u4e00\u767e\u679a\u7b26\u5408\u7b5b\u9009\u6761\u4ef6\u7684\u5206\u5b50\uff0c\u4e14\u5df2\u7ecf\u901a\u8fc7\u7ec4\u7ec7\u548c\u6838\u4f4d\u7f6e\u8ffd\u8e2a\u5230\u4e86\u540c\u4e00\u5bf9\u76f8\u90bb\u7ec6\u80de\u3002\u65e7\u8fb9\u754c\u7ed9\u4e0a\u76ae\u7ec6\u80de\u516d\u5341\u679a\u3001\u514d\u75ab\u7ec6\u80de\u4e8c\u5341\u679a\uff0c\u53e6\u6709\u4e8c\u5341\u679a\u672a\u5206\u914d\u3002\u65b0\u8fb9\u754c\u8ba9\u4e0a\u76ae\u7684\u516b\u679a\u5212\u5165\u514d\u75ab\u7ec6\u80de\uff0c\u8ba9\u539f\u6765\u672a\u5206\u914d\u7684\u56db\u679a\u5212\u5165\u4e0a\u76ae\uff0c\u5176\u4f59\u5206\u5b50\u4e0d\u52a8\u3002\u7ed3\u679c\u4e0a\u76ae\u5f97\u5230\u4e94\u5341\u516d\u679a\uff0c\u514d\u75ab\u5f97\u5230\u4e8c\u5341\u516b\u679a\uff0c\u672a\u5206\u914d\u5341\u516d\u679a\u3002\u4e0a\u76ae\u8868\u9762\u4e0a\u53ea\u51cf\u5c11\u56db\u679a\uff0c\u5b9e\u9645\u79bb\u5f00\u5b83\u7684\u5374\u6709\u516b\u679a\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n
\u65e7\u5f52\u5c5e \u2192 \u65b0\u5f52\u5c5e<\/th>\n\u4e0a\u76ae<\/th>\n\u514d\u75ab<\/th>\n\u672a\u5206\u914d<\/th>\n\u65e7\u5408\u8ba1<\/th>\n<\/tr>\n<\/thead>\n
\u4e0a\u76ae<\/td>\n52<\/td>\n8<\/td>\n0<\/td>\n60<\/td>\n<\/tr>\n
\u514d\u75ab<\/td>\n0<\/td>\n20<\/td>\n0<\/td>\n20<\/td>\n<\/tr>\n
\u672a\u5206\u914d<\/td>\n4<\/td>\n0<\/td>\n16<\/td>\n20<\/td>\n<\/tr>\n
\u65b0\u5408\u8ba1<\/td>\n56<\/td>\n28<\/td>\n16<\/td>\n100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u5230\u5e95\u6709\u51e0\u679a\u5206\u5b50\u8d8a\u8fc7\u4e86\u8fb9\u754c\u3002\u6559\u5b66\u7b97\u4f8b\uff5c\u76f8\u540c\u7684\u4e00\u767e\u679a\u5206\u5b50\uff0c\u4e24\u7248\u5206\u5272\u7684\u6700\u7ec8\u8ba1\u6570\u4e0d\u540c\u30028
\u6559\u5b66\u7b97\u4f8b\uff5c\u76f8\u540c\u7684\u4e00\u767e\u679a\u5206\u5b50\uff0c\u4e24\u7248\u5206\u5272\u7684\u6700\u7ec8\u8ba1\u6570\u4e0d\u540c\u3002<\/figcaption><\/figure>\n

\u6cbf\u884c\u8bfb\u662f\u539f\u6765\u8fd9\u7c7b\u5206\u5b50\u7684\u53bb\u5411\uff0c\u6cbf\u5217\u8bfb\u662f\u65b0\u5f52\u5c5e\u7684\u6765\u6e90\u3002\u6559\u5b66\u7b97\u4f8b\u4e2d\u603b\u4f53\u5df2\u5206\u914d\u6bd4\u4f8b\u4ece\u516b\u6210\u5347\u5230\u516b\u6210\u56db\uff0c\u4f46\u8fd9\u53ea\u8bf4\u660e\u66f4\u5c11\u5206\u5b50\u843d\u5728\u6240\u6709\u7ec6\u80de\u4e4b\u5916\uff0c\u4e0d\u80fd\u8bf4\u660e\u90a3\u516b\u4e2a\u65b0\u8fdb\u5165\u514d\u75ab\u7ec6\u80de\u7684\u5206\u5b50\u4e00\u5b9a\u88ab\u5206\u5bf9\u3002\u6269\u5927\u8fb9\u754c\u5c31\u53ef\u4ee5\u8ba9\u5206\u914d\u7387\u4e0a\u5347\uff0c\u540c\u65f6\u4e5f\u6709\u673a\u4f1a\u628a\u6765\u81ea\u9694\u58c1\u7684\u4fe1\u53f7\u5e26\u8fdb\u6765\u3002\u6bd4\u8f83\u65b9\u6848\u65f6\u5e94\u540c\u65f6\u62a5\u544a\u7559\u5728\u539f\u7fa4\u3001\u8de8\u7fa4\u8f6c\u79fb\u548c\u7531\u672a\u5206\u914d\u8f6c\u5165\u7684\u6570\u76ee\u3002\u53ea\u770b\u65b0\u77e9\u9635\u6bcf\u5217\u603b\u91cf\u4f1a\u4e22\u5931\u8fd9\u4e09\u4e2a\u6765\u6e90\u3002<\/p>\n

\u539f\u6765\u7684\u7ec6\u80de\u7f16\u53f7\u8fd8\u80fd\u4e0d\u80fd\u76f4\u63a5\u7528<\/h2>\n

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\u9010\u5206\u5b50\u8ffd\u8e2a\u65f6\uff0c\u4ee5\u539f\u5b9e\u9a8c\u7684\u5206\u5b50\u6807\u8bc6\u4f5c\u8fde\u63a5\u952e\uff0c\u5e76\u68c0\u67e5\u91cd\u590d\u548c\u7f3a\u5931\u3002\u7528\u5916\u8fde\u63a5\u5148\u770b\u6e05\u4e24\u7248\u5230\u5e95\u7f3a\u4e86\u4ec0\u4e48\uff0c\u518d\u5bf9\u4e24\u4fa7\u90fd\u51fa\u73b0\u7684\u5206\u5b50\u7f16\u53f7\u5efa\u7acb\u53bb\u5411\u8868\u3002\u4e0b\u9762\u5047\u5b9a\u4e24\u5f20\u7ecf\u8d28\u91cf\u7b5b\u9009\u7684\u8868\u90fd\u542b\u5206\u5b50\u6807\u8bc6\u548c\u5df2\u7ecf\u6838\u5b9e\u3001\u53ef\u4ee5\u8de8\u7248\u6bd4\u8f83\u7684\u7ec6\u80de\u7c7b\u522b\uff0c\u5206\u5b50\u672a\u5206\u914d\u65f6\u7c7b\u522b\u4e3a\u7a7a\u3002\u82e5\u67d0\u4e2a\u5206\u5b50\u53ea\u51fa\u73b0\u5728\u4e00\u4efd\u6587\u4ef6\u4e2d\uff0c\u5e94\u53e6\u67e5\u8fc7\u6ee4\u539f\u56e0\uff0c\u4e0d\u80fd\u628a\u5b83\u5f53\u4f5c\u201c\u5206\u5b50\u4ecd\u5728\uff0c\u4f46\u672a\u5206\u914d\u201d\u3002<\/p>\n

import pandas as pd\r\n\r\njoint = old.merge(new, on="transcript_id", how="outer",\r\n                  suffixes=("_old", "_new"), indicator=True,\r\n                  validate="one_to_one")\r\nprint(joint["_merge"].value_counts())\r\nboth = joint.loc[joint["_merge"].eq("both")]\r\nprint(pd.crosstab(both["cell_type_old"].fillna("unassigned"),\r\n                  both["cell_type_new"].fillna("unassigned")))\r\n<\/code><\/pre>\n
\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u8ffd\u8e2a\u4e94\u4e07\u6761\u516c\u5f00\u4e73\u817a\u7ec4\u7ec7\u5206\u5b50\u8bb0\u5f55<\/h2>\n
\u6211\u4eec\u7528 pandas 3.1.1 \u8bfb\u53d6\u5b98\u65b9\u4eba\u4e73\u817a\u764c\u6837\u672c\u7b2c\u4e00\u4e2a\u6280\u672f\u91cd\u590d\u7684\u8f6c\u5f55\u672c\u6587\u4ef6\uff0c\u56fa\u5b9a\u53d6\u6587\u4ef6\u5f00\u5934\u4e94\u4e07\u6761\u3002\u4e94\u4e07\u6761\u7f16\u53f7\u4e92\u4e0d\u91cd\u590d\uff0c\u901a\u8fc7\u8d28\u91cf\u503c\u4e8c\u5341\u8fd9\u4e00\u95e8\u69db\u7684\u6709 41,025 \u6761\uff0c\u5176\u4e2d\u5341\u4e5d\u6761\u4e3a\u5bf9\u7167\u7279\u5f81\u3002\u5176\u4f59 41,006 \u6761\u8fdb\u5165\u6bd4\u8f83\u3002\u8fd9\u4e2a\u65e9\u671f\u6587\u4ef6\u7528\u8d1f\u4e00\u8868\u793a\u672a\u5206\u914d\uff0c\u5982\u679c\u53ea\u627e\u7a7a\u503c\uff0c\u4f1a\u628a\u539f\u672c\u6ca1\u6709\u6240\u5c5e\u7ec6\u80de\u7684\u4e5d\u5341\u56db\u6761\u4e5f\u7b97\u6210\u5df2\u5206\u914d\u3002\u590d\u7528\u6587\u4e2d\u7684\u8fde\u63a5\u4ee3\u7801\u65f6\uff0c\u8981\u5148\u628a\u5f53\u524d\u7248\u672c\u6587\u4ef6\u4e2d\u7684\u672a\u5206\u914d\u6807\u8bc6\u8f6c\u6210\u7edf\u4e00\u7684\u7a7a\u503c\u3002\u8be5\u6837\u672c\u7684\u539f\u7814\u7a76\u6570\u636e\u548c\u6587\u4ef6\u6765\u6e90<\/a> \u00b7 \u6240\u7528\u6587\u4ef6\u7684\u5217\u5b9a\u4e49<\/a><\/p>\n
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