{"id":69869,"date":"2026-11-14T21:57:44","date_gmt":"2026-11-14T13:57:44","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=69869"},"modified":"2026-11-14T21:57:44","modified_gmt":"2026-11-14T13:57:44","slug":"paste-transport-cropped-section-missing-spot","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/paste-transport-cropped-section-missing-spot\/","title":{"rendered":"PASTE \u5bf9\u9f50\u4e24\u5f20\u5207\u7247\uff0c\u4e3a\u4ec0\u4e48\u5df2\u7ecf\u88c1\u6389\u7684\u533a\u57df\u4ecd\u7136\u6709\u5339\u914d\uff1f"},"content":{"rendered":"
\n

\u4e24\u5f20\u76f8\u90bb\u5207\u7247\u5e76\u6392\u6446\u5728\u5c4f\u5e55\u4e0a\uff0c\u5de6\u56fe\u6709\u4e00\u5c0f\u5757\u7ec4\u7ec7\u4f38\u51fa\uff0c\u53f3\u56fe\u5728\u76f8\u5e94\u4f4d\u7f6e\u6070\u597d\u88c1\u6389\u4e86\u4e00\u89d2\u3002\u8fd0\u884c\u5bf9\u9f50\u7a0b\u5e8f\u540e\uff0c\u5de6\u56fe\u8fd9\u5757\u7ec4\u7ec7\u4ecd\u5411\u53f3\u56fe\u53d1\u51fa\u51e0\u6761\u8fde\u7ebf\u3002\u5b83\u662f\u5426\u5728\u53e6\u4e00\u5f20\u7247\u4e0a\u8fd8\u6709\u5bf9\u5e94\u7684\u7ec6\u80de\u7fa4\uff1f\u8981\u56de\u7b54\u8fd9\u4e2a\u95ee\u9898\uff0c\u5148\u770b\u8f93\u51fa\u77e9\u9635\u5728\u6570\u5b66\u4e0a\u5fc5\u987b\u6ee1\u8db3\u7684\u6761\u4ef6\u3002\u539f\u59cb PASTE \u7684\u4e24\u7247\u5bf9\u9f50\u8f93\u51fa\u63cf\u8ff0\u4e24\u5f20\u5207\u7247\u7684\u70b9\u4f4d\u8d28\u91cf\u600e\u6837\u5206\u914d\u3002\u82e5\u6bcf\u4e2a\u70b9\u90fd\u5fc5\u987b\u628a\u5206\u914d\u989d\u5ea6\u7528\u5b8c\uff0c\u5373\u4f7f\u53e6\u4e00\u7247\u7f3a\u4e86\u4e00\u89d2\uff0c\u4e5f\u4f9d\u7136\u80fd\u5f97\u5230\u67d0\u79cd\u5206\u914d\u3002\u8fd9\u4f1a\u76f4\u63a5\u5f71\u54cd\u5982\u4f55\u9605\u8bfb\u5f69\u8272\u8fde\u7ebf\u3001\u6700\u5927\u5339\u914d\u548c\u53e0\u5408\u56fe\u3002PASTE \u539f\u59cb\u65b9\u6cd5<\/a><\/p>\n

\u7ed9\u4f20\u8f93\u77e9\u9635\u5199\u4e0a\u884c\u540d\u3001\u5217\u540d\u548c\u5206\u6bcd<\/h2>\n

\u8bbe\u77e9\u9635\u7684\u884c\u662f\u6765\u6e90\u5207\u7247\u4e0a\u7684\u6355\u83b7\u70b9\uff0c\u5217\u662f\u76ee\u6807\u5207\u7247\u4e0a\u7684\u6355\u83b7\u70b9\u3002\u8f6f\u4ef6\u7ed3\u5408\u4e24\u79cd\u4fe1\u606f\u4f30\u8ba1\u77e9\u9635\uff0c\u4e00\u79cd\u662f\u8de8\u7247\u8868\u8fbe\u7279\u5f81\u5dee\u5f02\uff0c\u53e6\u4e00\u79cd\u662f\u5404\u7247\u5185\u90e8\u7684\u70b9\u95f4\u51e0\u4f55\u5173\u7cfb\u3002\u4f7f\u7528\u540c\u7247\u76f8\u5bf9\u8ddd\u79bb\u6709\u4e00\u4e2a\u5177\u4f53\u597d\u5904\uff0c\u4e00\u5f20\u7247\u5373\u4f7f\u6574\u4f53\u65cb\u8f6c\u548c\u5e73\u79fb\uff0c\u5185\u90e8\u4efb\u610f\u4e24\u70b9\u95f4\u7684\u8ddd\u79bb\u4ecd\u7136\u4fdd\u6301\u4e0d\u53d8\u3002\u82e5\u4e00\u5f20\u7247\u7684\u5750\u6807\u5355\u4f4d\u662f\u50cf\u7d20\uff0c\u53e6\u4e00\u5f20\u662f\u5fae\u7c73\uff0c\u6216\u8005\u7ecf\u5386\u4e86\u4e0d\u5747\u5300\u7684\u5f62\u53d8\uff0c\u51e0\u4f55\u5173\u7cfb\u5c31\u672a\u5fc5\u80fd\u76f4\u63a5\u6bd4\u8f83\u3002\u5b9e\u9645\u5bf9\u9f50\u65f6\u5e94\u4fdd\u7559\u5750\u6807\u5355\u4f4d\u548c\u539f\u59cb\u7167\u7247\uff0c\u5e76\u5148\u786e\u8ba4\u4e24\u5f20\u7247\u53d6\u7684\u662f\u540c\u4e00\u89e3\u5256\u5c42\u9762\u7684\u8db3\u591f\u91cd\u53e0\u533a\u57df\u3002<\/p>\n

\u5728\u9ed8\u8ba4\u5747\u5300\u8fb9\u9645\u7684\u4f8b\u5b50\u91cc\uff0c\u82e5\u6765\u6e90\u6709\u516d\u4e2a\u70b9\uff0c\u6bcf\u884c\u5e94\u52a0\u5230\u516d\u5206\u4e4b\u4e00\u3002\u76ee\u6807\u82e5\u6709\u4e94\u4e2a\u70b9\uff0c\u6bcf\u5217\u5e94\u52a0\u5230\u4e94\u5206\u4e4b\u4e00\u3002\u503c\u4e3a\u96f6\u70b9\u4e00\u516d\u4e03\u7684\u683c\u5b50\u53ef\u80fd\u5df2\u7ecf\u8017\u5c3d\u4e86\u8fd9\u4e00\u884c\u7684\u5168\u90e8\u989d\u5ea6\uff0c\u4e0d\u80fd\u770b\u5230\u5b83\u5c0f\u4e8e\u4e00\u70b9\u96f6\u5c31\u89e3\u91ca\u4e3a\u53ea\u6709\u4e0d\u8db3\u4e24\u6210\u628a\u63e1\u3002\u82e5\u8981\u63cf\u8ff0\u67d0\u4e2a\u6e90\u70b9\u6d41\u5411\u5404\u76ee\u6807\u70b9\u7684\u76f8\u5bf9\u5360\u6bd4\uff0c\u5148\u5c06\u8be5\u884c\u9664\u4ee5\u5b83\u7684\u548c\u3002\u8fd9\u4e2a\u5360\u6bd4\u53d7\u7ed9\u5b9a\u8868\u8fbe\u3001\u51e0\u4f55\u548c\u8fb9\u9645\u7ea6\u675f\u5171\u540c\u5f71\u54cd\uff0c\u4e0d\u80fd\u76f4\u63a5\u5f53\u4f5c\u751f\u7269\u540c\u6e90\u6982\u7387\u3002\u5bf9\u7167\u4e24\u4e2a\u5206\u6bcd\uff0c\u662f\u8bfb\u8fd9\u79cd\u8868\u683c\u6700\u7701\u65f6\u95f4\u7684\u4e00\u6b65\u3002\u4f5c\u8005\u5305\u7684\u914d\u5bf9\u63a5\u53e3\u548c\u8fb9\u9645\u53c2\u6570<\/a><\/p>\n

\n\n\n\n\n\n\n\n
\u8bbe\u7f6e<\/th>\n\u6700\u5927\u5339\u914d\u80fd\u8bf4\u660e\u4ec0\u4e48<\/th>\n\u5982\u4f55\u68c0\u67e5<\/th>\n<\/tr>\n<\/thead>\n
\u4e24\u7247\u6709\u5bc6\u5b9e\u4e14\u5355\u4e00\u7684\u5bf9\u5e94<\/td>\n\u5728\u5f53\u524d\u7ea6\u675f\u4e0b\u8d28\u91cf\u96c6\u4e2d<\/td>\n\u6bd4\u8f83\u884c\u5185\u7b2c\u4e00\u3001\u7b2c\u4e8c\u5019\u9009\u53ca\u72ec\u7acb\u5730\u6807<\/td>\n<\/tr>\n
\u6e90\u7247\u6709\u4e00\u5757\u5728\u76ee\u6807\u7247\u7f3a\u5931<\/td>\n\u8fd9\u4e00\u884c\u4ecd\u9700\u5206\u914d\u989d\u5ea6<\/td>\n\u770b\u6574\u884c\u548c\u5b9e\u9645\u91cd\u53e0\u63a9\u819c<\/td>\n<\/tr>\n
\u4e24\u4e2a\u76ee\u6807\u70b9\u5e76\u5217\u6216\u8fd1\u4f3c\u5e76\u5217<\/td>\n\u5355\u4e2a\u6700\u5927\u7f16\u53f7\u53d7\u5217\u987a\u5e8f\u6216\u5fae\u5c0f\u8bef\u5dee\u5f71\u54cd<\/td>\n\u4fdd\u5b58\u6240\u6709\u8fd1\u4f3c\u5e76\u5217\u7684\u70b9\u548c\u5bf9\u5e94\u4efd\u989d<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u88c1\u6389\u7684\u70b9\u4e3a\u4ec0\u4e48\u8fd8\u6709\u8fde\u7ebf\u3002\u5e73\u8861\u4f20\u8f93\u5b9e\u8dd1\uff5c\u4e24\u5f20\u6a21\u62df\u7247\u5148\u5b8c\u6574\u914d\u5bf9\uff0c\u518d\u79fb\u53bb\u4e00\u4e2a\u5df2\u77e5\u70b9\u3002\u5b8c\u6574\uff0c6
\u5e73\u8861\u4f20\u8f93\u5b9e\u8dd1\uff5c\u4e24\u5f20\u6a21\u62df\u7247\u5148\u5b8c\u6574\u914d\u5bf9\uff0c\u518d\u79fb\u53bb\u4e00\u4e2a\u5df2\u77e5\u70b9\u3002<\/figcaption><\/figure>\n

\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u5220\u9664\u4e00\u4e2a\u771f\u5b9e\u5bf9\u5e94\u70b9\uff0c\u77e9\u9635\u628a\u8fd9\u4e00\u884c\u653e\u5230\u4e86\u54ea\u91cc<\/h2>\n

\u6211\u4eec\u5b89\u88c5 paste-bio 1.4.0\uff0c\u7528\u516d\u4e2a\u975e\u5b8c\u5168\u5bf9\u79f0\u6392\u5217\u7684\u70b9\u548c\u5341\u4e8c\u4e2a\u57fa\u56e0\u6784\u9020\u6a21\u62df\u5207\u7247\u3002\u6bcf\u4e2a\u70b9\u62e5\u6709\u4e00\u5bf9\u76f8\u5bf9\u7a81\u51fa\u7684\u8868\u8fbe\u7279\u5f81\u3002\u590d\u5236\u5207\u7247\u65f6\uff0c\u4fdd\u6301\u8868\u8fbe\u5411\u91cf\u4e0d\u53d8\uff0c\u5c06\u5750\u6807\u65cb\u8f6c\u4e5d\u5341\u5ea6\u5e76\u5e73\u79fb\uff0c\u518d\u6545\u610f\u6253\u4e71\u76ee\u6807\u70b9\u7684\u5b58\u50a8\u987a\u5e8f\u3002\u4e24\u7247\u7684\u6240\u6709\u70b9\u540d\u5728\u8bd5\u9a8c\u8bb0\u5f55\u4e2d\u90fd\u6709\u5df2\u77e5\u5bf9\u5e94\uff0c\u56e0\u800c\u65e2\u80fd\u8003\u5bdf\u65cb\u8f6c\u5e73\u79fb\uff0c\u4e5f\u80fd\u68c0\u9a8c\u811a\u672c\u6709\u6ca1\u6709\u5077\u7528\u76f8\u540c\u884c\u53f7\u3002\u4f7f\u7528\u5747\u5300\u8fb9\u9645\u3001\u6b27\u6c0f\u8868\u8fbe\u5dee\u5f02\uff0c\u7a7a\u95f4\u9879\u6743\u91cd\u8bbe\u4e3a\u96f6\u70b9\u4e00\uff0c\u5e76\u542f\u7528\u7a7a\u95f4\u8ddd\u79bb\u5f52\u4e00\u5316\u3002\u5b8c\u6574\u60c5\u51b5\u4e0b\uff0c\u516d\u4e2a\u6e90\u70b9\u7684\u6700\u5927\u8d28\u91cf\u90fd\u5bf9\u5e94\u5230\u6b63\u786e\u76ee\u6807\uff0c\u6bcf\u884c\u53ea\u6709\u4e00\u4e2a\u7ea6\u516d\u5206\u4e4b\u4e00\u7684\u975e\u96f6\u9879\u3002<\/p>\n

\u7b2c\u4e8c\u6b21\u4ec5\u4ece\u76ee\u6807\u7247\u5220\u9664\u6e90\u7f16\u53f7\u4e94\u7684\u526f\u672c\uff0c\u5176\u4ed6\u4e94\u5bf9\u4ecd\u7136\u4e00\u4e00\u5bf9\u5e94\u3002\u91cd\u65b0\u8fd0\u884c\u540e\uff0c\u8fd9\u4e94\u5bf9\u7684\u6700\u5927\u5339\u914d\u5168\u90e8\u6b63\u786e\uff0c\u4f46\u6e90\u7f16\u53f7\u4e94\u7684\u90a3\u4e00\u884c\u5411\u4e94\u4e2a\u76ee\u6807\u5404\u5206\u914d\u7ea6\u96f6\u70b9\u96f6\u4e09\u4e09\u4e09\u4e09\uff0c\u603b\u548c\u4ecd\u4e3a\u516d\u5206\u4e4b\u4e00\u3002\u6362\u7b97\u6210\u8fd9\u4e00\u884c\u5185\u90e8\u7684\u6bd4\u4f8b\uff0c\u6bcf\u4e2a\u76ee\u6807\u7ea6\u5360\u4e94\u5206\u4e4b\u4e00\u3002\u82e5\u811a\u672c\u53ea\u5bfc\u51fa\u4e00\u5217\u6700\u5927\u4f4d\u7f6e\uff0c\u5b83\u4f9d\u7136\u4f1a\u7ed9\u51fa\u67d0\u4e2a\u5177\u4f53\u7f16\u53f7\uff0c\u5374\u4f1a\u9690\u85cf\u8fd9\u4e2a\u6e90\u70b9\u5bf9\u4e94\u4e2a\u4f4d\u7f6e\u51e0\u4e4e\u7b49\u91cf\u5206\u914d\u7684\u4e8b\u5b9e\u3002\u8fd9\u4e2a\u4f8b\u5b50\u53ef\u4ee5\u624b\u7b97\u6838\u5bf9\u3002\u6bcf\u4e2a\u76ee\u6807\u987b\u6536\u5230\u4e94\u5206\u4e4b\u4e00\uff0c\u5df2\u6709\u7684\u540c\u6e90\u70b9\u8d21\u732e\u516d\u5206\u4e4b\u4e00\uff0c\u4f59\u4e0b\u7684\u4e09\u5341\u5206\u4e4b\u4e00\u6b63\u597d\u7531\u7f3a\u5c11\u5bf9\u5e94\u7684\u6e90\u70b9\u8865\u9f50\u3002<\/p>\n

import numpy as np\r\n\r\n# pi \u6765\u81ea\u4e00\u6b21 pairwise_align\uff0c\u884c\u3001\u5217\u6b21\u5e8f\u5206\u522b\u6765\u81ea\u5f53\u65f6\u7684\u4e24\u4e2a\u8f93\u5165\u5bf9\u8c61\r\nrow_sum = pi.sum(axis=1, keepdims=True)\r\nshare = np.divide(pi, row_sum, out=np.zeros_like(pi), where=row_sum > 0)\r\nranked = np.sort(share, axis=1)\r\nprint(pi.sum(axis=0), row_sum.ravel())\r\nprint(ranked[:, -1], ranked[:, -1] - ranked[:, -2])\r\n<\/code><\/pre>\n
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