{"id":69944,"date":"2026-11-14T22:13:20","date_gmt":"2026-11-14T14:13:20","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=69944"},"modified":"2026-11-14T22:13:20","modified_gmt":"2026-11-14T14:13:20","slug":"qupath-nested-annotations-cell-density-pixel-calibration","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/qupath-nested-annotations-cell-density-pixel-calibration\/","title":{"rendered":"QuPath \u7684\u5927\u533a\u57df\u91cc\u660e\u660e\u6709\u5341\u4e2a\u5bf9\u8c61\uff0c\u4e3a\u4ec0\u4e48\u6309\u5b50\u5bf9\u8c61\u53ea\u6570\u5230\u56db\u4e2a\uff1f"},"content":{"rendered":"
\n

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\n\n\n\n\n\n\n\n\n
\u91cf\u4ec0\u4e48<\/th>\n\u6574\u5757\u5927\u533a<\/th>\n\u5185\u5d4c\u6838\u5fc3\u533a<\/th>\n<\/tr>\n<\/thead>\n
\u76f4\u63a5\u6302\u5728\u6807\u6ce8\u4e0b\u7684\u68c0\u6d4b\u6570<\/td>\n4<\/td>\n6<\/td>\n<\/tr>\n
\u4e2d\u5fc3\u843d\u5728\u8303\u56f4\u5185\u7684\u5168\u90e8\u68c0\u6d4b\u6570<\/td>\n10<\/td>\n6<\/td>\n<\/tr>\n
\u539f\u59cb\u50cf\u7d20\u9762\u79ef<\/td>\n6000 \u50cf\u7d20\u00b2<\/td>\n2400 \u50cf\u7d20\u00b2<\/td>\n<\/tr>\n
\u6bcf\u50cf\u7d20 0.5 \u5fae\u7c73\u65f6\u7684\u9762\u79ef<\/td>\n1500 \u5fae\u7c73\u00b2<\/td>\n600 \u5fae\u7c73\u00b2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u5927\u6846\u91cc\u6709\u5341\u4e2a\uff0c\u4e3a\u4ec0\u4e48\u53ea\u6570\u5230\u56db\u4e2a\u3002\u5bf9\u8c61\u5c42\u7ea7\u5b9e\u8dd1\uff5c\u540c\u4e00\u7ec4\u692d\u5706\u653e\u5165\u5d4c\u5957\u6807\u6ce8\uff0c\u53e6\u5b58\u6709\u65e0\u6807\u5b9a\u7684\u4e24\u4efd\u56fe\u3002\u76f4\u63a5\u4e0b\u7ea7\uff0c\u5916\u5c42
\u5bf9\u8c61\u5c42\u7ea7\u5b9e\u8dd1\uff5c\u540c\u4e00\u7ec4\u692d\u5706\u653e\u5165\u5d4c\u5957\u6807\u6ce8\uff0c\u53e6\u5b58\u6709\u65e0\u6807\u5b9a\u7684\u4e24\u4efd\u56fe\u3002<\/figcaption><\/figure>\n

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def roi = annotation.getROI()\r\ndef calibration = getCurrentServer().getPixelCalibration()\r\ndef contained = getDetectionObjects().count {\r\n    def det = it.getROI()\r\n    roi.contains(det.getCentroidX(), det.getCentroidY())\r\n}\r\ndef area = calibration.hasPixelSizeMicrons() ?\r\n    roi.getScaledArea(calibration.getPixelWidthMicrons(), calibration.getPixelHeightMicrons()) : null\r\nprintln([centerCount: contained, areaMicronsSquared: area])\r\n<\/code><\/pre>\n
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