{"id":70029,"date":"2026-11-14T22:49:34","date_gmt":"2026-11-14T14:49:34","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=70029"},"modified":"2026-11-14T22:49:34","modified_gmt":"2026-11-14T14:49:34","slug":"fastqc-duplication-prefix-50bp-versus-full-read","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/fastqc-duplication-prefix-50bp-versus-full-read\/","title":{"rendered":"\u4e24\u5343\u6761\u5b8c\u6574\u5e8f\u5217\u5404\u4e0d\u76f8\u540c\uff0cFastQC \u4e3a\u4ec0\u4e48\u53ea\u62a5 0.05% \u7684\u53bb\u91cd\u540e\u6bd4\u4f8b\uff1f"},"content":{"rendered":"<div class=\"ysx-method-guide\" style=\"max-width:820px;min-width:0;margin:0 auto;color:#26374a;line-height:1.86;overflow-wrap:anywhere\">\n<p>\u8d28\u63a7\u62a5\u544a\u91cc\u201c\u5e8f\u5217\u91cd\u590d\u201d\u4e00\u680f\u53d8\u7ea2\uff0c\u80fd\u4e0d\u80fd\u636e\u6b64\u5224\u65ad\u6587\u5e93\u88ab\u4e25\u91cd\u6269\u589e\uff0c\u5e94\u8be5\u9a6c\u4e0a\u53bb\u91cd\u590d\uff1f\u5148\u62ff\u4e00\u4e2a\u53cd\u4f8b\u8bd5\u8bd5\u3002\u6211\u4eec\u751f\u6210\u4e24\u5343\u6761\u4e03\u5341\u4e94\u78b1\u57fa\u7684\u6a21\u62df\u8bfb\u6bb5\uff0c\u9010\u4e00\u68c0\u67e5\uff0c\u5168\u957f\u5e8f\u5217\u6ca1\u6709\u4efb\u4f55\u4e24\u6761\u76f8\u540c\u3002FastQC 0.12.1 \u7684\u9ed8\u8ba4\u62a5\u544a\u5374\u7ed9\u51fa\u767e\u5206\u4e4b\u96f6\u70b9\u96f6\u4e94\u7684\u53bb\u91cd\u540e\u6bd4\u4f8b\uff0c\u800c\u4e14\u628a\u540c\u4e00\u4e2a\u4e94\u5341\u78b1\u57fa\u7247\u6bb5\u5217\u4e3a\u767e\u5206\u4e4b\u767e\u8fc7\u5ea6\u4ee3\u8868\u3002\u56fa\u5b9a\u8fd9\u4efd FASTQ\uff0c\u53ea\u6539\u53d8\u7edf\u8ba1\u6240\u6bd4\u8f83\u7684\u5e8f\u5217\u957f\u5ea6\uff0c\u53bb\u91cd\u540e\u6bd4\u4f8b\u53c8\u56de\u5230\u767e\u5206\u4e4b\u767e\u3002\u8fd9\u4e2a\u7ec3\u4e60\u53ef\u4ee5\u5e2e\u4eba\u5206\u6e05\u8d28\u91cf\u62a5\u544a\u8bc6\u522b\u5230\u7684\u6a21\u5f0f\uff0c\u4e0e\u9020\u6210\u6a21\u5f0f\u7684\u5b9e\u9a8c\u539f\u56e0\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u8bfb\u61c2\u53bb\u91cd\u540e\u6bd4\u4f8b\u7684\u5206\u5b50\u4e0e\u5206\u6bcd<\/h2>\n<p>\u628a\u201c\u53bb\u91cd\u540e\u6bd4\u4f8b\u201d\u7406\u89e3\u6210\u4e00\u4e2a\u53cd\u4e8b\u5b9e\u7edf\u8ba1\u6bd4\u8f83\u76f4\u89c2\u3002\u5047\u8bbe\u6309\u7167\u672c\u6a21\u5757\u5bf9\u5e8f\u5217\u76f8\u540c\u7684\u5b9a\u4e49\uff0c\u6bcf\u4e00\u79cd\u5e8f\u5217\u53ea\u4fdd\u7559\u4e00\u6761\uff0c\u6570\u91cf\u4f1a\u662f\u539f\u59cb\u8bfb\u6bb5\u6570\u7684\u591a\u5c11\u3002\u5bf9\u4e8e\u4e24\u5343\u6761\u5404\u4e0d\u76f8\u540c\u7684\u6bd4\u8f83\u5e8f\u5217\uff0c\u53bb\u91cd\u540e\u8fd8\u6709\u4e24\u5343\u6761\uff0c\u5373\u767e\u5206\u4e4b\u767e\u3002\u82e5\u4e24\u5343\u6761\u5e8f\u5217\u5728\u6a21\u5757\u4f7f\u7528\u7684\u8303\u56f4\u91cc\u5168\u90fd\u76f8\u540c\uff0c\u53bb\u91cd\u540e\u53ea\u5269\u4e00\u4e2a\u7c7b\u522b\uff0c\u5373\u4e00\u9664\u4ee5\u4e24\u5343\uff0c\u4e5f\u5c31\u662f\u767e\u5206\u4e4b\u96f6\u70b9\u96f6\u4e94\u3002FastQC \u53ea\u662f\u5728\u62a5\u544a\u4e2d\u4f30\u8ba1\u548c\u5c55\u793a\u8fd9\u6837\u7684\u91cd\u590d\u6a21\u5f0f\uff0c\u5b83\u4e0d\u4f1a\u5bf9\u8f93\u5165 FASTQ \u5b9e\u9645\u5220\u9664\u8bfb\u6bb5\u3002\u5bf9\u4e8e\u7279\u522b\u5927\u7684\u6587\u4ef6\uff0c\u8be5\u6a21\u5757\u8fd8\u6709\u9650\u5236\u5185\u5b58\u7528\u91cf\u7684\u8ddf\u8e2a\u7b56\u7565\uff0c\u4e0d\u80fd\u5c06\u6240\u6709\u573a\u666f\u90fd\u89c6\u4e3a\u5bf9\u6574\u5e93\u6bcf\u4e2a\u5168\u957f\u5e8f\u5217\u7684\u7cbe\u786e\u67e5\u91cd\u3002<a style=\"color:#43619b;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/Help\/3%20Analysis%20Modules\/8%20Duplicate%20Sequences.html\">FastQC \u7684\u5e8f\u5217\u91cd\u590d\u6a21\u5757\u8bf4\u660e<\/a><\/p>\n<p>\u9009\u62e9\u6bd4\u8f83\u8303\u56f4\u4f1a\u6539\u53d8\u201c\u540c\u4e00\u79cd\u201d\u7684\u5b9a\u4e49\u3002\u8bfb\u6bb5\u5f00\u5934\u5982\u679c\u5305\u542b\u5171\u540c\u5f15\u7269\u3001\u56fa\u5b9a\u524d\u5bfc\u5e8f\u5217\u6216\u67d0\u4e9b\u5efa\u5e93\u6280\u672f\u8981\u6c42\u7684\u4e00\u81f4\u5e8f\u5217\uff0c\u800c\u771f\u6b63\u533a\u5206\u7247\u6bb5\u7684\u4fe1\u606f\u5728\u540e\u534a\u622a\uff0c\u90a3\u4e48\u7edf\u8ba1\u6574\u4e2a\u8bfb\u957f\u4e0e\u4ec5\u6bd4\u8f83\u524d\u6bb5\u4f1a\u7ed9\u51fa\u4e0d\u540c\u7b54\u6848\u3002\u53e6\u4e00\u4e2a\u5e38\u89c1\u573a\u666f\u662f\u6838\u7cd6\u4f53\u6216\u9ad8\u4e30\u5ea6\u8f6c\u5f55\u672c\u8d21\u732e\u4e86\u5927\u91cf\u76f8\u540c\u7684\u8bfb\u6bb5\uff0c\u5e8f\u5217\u786e\u5b9e\u53ef\u80fd\u91cd\u590d\uff0c\u4f46\u91cd\u590d\u6765\u6e90\u4ecd\u53ef\u80fd\u5305\u542b\u771f\u5b9e\u7684\u751f\u7269\u4e30\u5ea6\u3002\u53ef\u5148\u770b\u662f\u5426\u4e3a\u968f\u673a\u6253\u65ad\u6587\u5e93\u3001\u662f\u5426\u9884\u671f\u56fa\u5b9a\u7aef\u3001\u6709\u6ca1\u6709\u552f\u4e00\u5206\u5b50\u6807\u8bb0\uff0c\u518d\u51b3\u5b9a\u8be5\u68c0\u67e5\u54ea\u4e00\u7c7b\u5b9e\u9a8c\u504f\u5dee\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u540c\u4e00\u4efd FASTQ\uff0c\u9ed8\u8ba4\u4e94\u5341\u78b1\u57fa\u5bf9\u6bd4\u5b8c\u6574\u4e03\u5341\u4e94\u78b1\u57fa<\/h2>\n<p>\u6211\u4eec\u5728 Ubuntu \u5b89\u88c5 FastQC 0.12.1\uff0c\u5148\u751f\u6210\u4e24\u5343\u6761\u72ec\u7acb\u62bd\u53d6\u7684\u4e03\u5341\u4e94\u78b1\u57fa\u8bfb\u6bb5\uff0c\u518d\u53e6\u9020\u4e00\u7ec4\u5171\u4eab\u524d\u4e94\u5341\u78b1\u57fa\u7684\u8bfb\u6bb5\uff0c\u540e\u9762\u4e8c\u5341\u4e94\u78b1\u57fa\u72ec\u7acb\u62bd\u53d6\u3002\u8fd0\u884c\u524d\u7528\u5b8c\u6574\u5b57\u7b26\u4e32\u96c6\u5408\u6838\u5b9e\uff0c\u4e24\u7ec4\u5b9e\u9645\u7684\u5168\u957f\u552f\u4e00\u5e8f\u5217\u6570\u5747\u4e3a\u4e24\u5343\u3002\u6bcf\u4e2a\u6848\u4f8b\u4f7f\u7528\u76f8\u540c\u7684\u8d28\u91cf\u8bbe\u8ba1\u548c\u53ef\u8ffd\u67e5\u7684\u8bfb\u6bb5\u7f16\u53f7\uff0c\u4ece\u8f6f\u4ef6\u751f\u6210\u7684\u538b\u7f29\u62a5\u544a\u91cc\u8bfb\u53d6\u91cd\u590d\u6a21\u5757\u3001\u8fc7\u5ea6\u4ee3\u8868\u5e8f\u5217\u6a21\u5757\u548c\u57fa\u672c\u7edf\u8ba1\u3002\u4f5c\u4e3a\u6b63\u5bf9\u7167\uff0c\u53e6\u9020\u4e24\u5343\u6761\u4e2d\u4e00\u5343\u6761\u5b8c\u5168\u76f8\u540c\u3001\u53e6\u5916\u4e00\u5343\u6761\u5f7c\u6b64\u4e0d\u540c\u4e14\u4e0d\u540c\u4e8e\u524d\u8005\u7684\u6587\u5e93\u3002<\/p>\n<p>\u968f\u673a\u5bf9\u7167\u7684\u53bb\u91cd\u540e\u6bd4\u4f8b\u4e3a\u767e\u5206\u4e4b\u767e\u3002\u5171\u4eab\u524d\u7f00\u7684\u4e03\u5341\u4e94\u78b1\u57fa\u6587\u5e93\u5728\u9ed8\u8ba4\u6a21\u5f0f\u4e0b\u4e3a\u767e\u5206\u4e4b\u96f6\u70b9\u96f6\u4e94\uff0c\u8fc7\u5ea6\u4ee3\u8868\u6a21\u5757\u5217\u51fa\u90a3\u4e2a\u4e94\u5341\u78b1\u57fa\u524d\u7f00\uff0c\u8ba1\u6570\u4e24\u5343\uff0c\u5360\u767e\u5206\u4e4b\u767e\u3002\u4f7f\u7528\u5b8c\u5168\u76f8\u540c\u7684\u4e03\u5341\u4e94\u78b1\u57fa\u5e8f\u5217\u548c\u8d28\u91cf\u5b57\u7b26\u4e32\uff0c\u5c06\u6bd4\u8f83\u957f\u5ea6\u663e\u5f0f\u8bbe\u4e3a\u4e03\u5341\u4e94\u540e\uff0c\u91cd\u590d\u6a21\u5757\u56de\u5230\u767e\u5206\u4e4b\u767e\uff0c\u8fc7\u5ea6\u4ee3\u8868\u5217\u8868\u4e5f\u6ca1\u6709\u8fd9\u4e00\u9879\u3002\u771f\u6b63\u7684\u4e00\u534a\u5b8c\u5168\u76f8\u540c\u6b63\u5bf9\u7167\u5f97\u5230\u767e\u5206\u4e4b\u4e94\u5341\u70b9\u96f6\u4e94\uff0c\u5bf9\u5e94\u4e00\u5343\u96f6\u4e00\u79cd\u5e8f\u5217\u9664\u4ee5\u4e24\u5343\u6761\u3002\u8fd9\u9879\u6838\u7b97\u628a\u201c\u8bfb\u6bb5\u540d\u91cd\u590d\u201d\u201c\u5b8c\u6574\u5e8f\u5217\u91cd\u590d\u201d\u548c\u201c\u7528\u4e8e\u7edf\u8ba1\u7684\u524d\u7f00\u91cd\u590d\u201d\u5206\u522b\u653e\u5230\u4e86\u53ef\u68c0\u67e5\u7684\u4f4d\u7f6e\u3002<\/p>\n<div class=\"guide-table\" style=\"max-width:100%;overflow-x:auto;margin:18px 0\">\n<table style=\"display:table;width:100%;max-width:100%;min-width:0;table-layout:fixed;border-collapse:collapse;font-size:.92em;line-height:1.6\">\n<thead>\n<tr>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#f0f5ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u5df2\u77e5\u8f93\u5165<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#f0f5ff;color:#1e293b;vertical-align:top;text-align:right;padding:6px;border:1px solid #c4cfdb\">\u9ed8\u8ba4\u53bb\u91cd\u540e\u6bd4\u4f8b<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#f0f5ff;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u89e3\u91ca<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u4e24\u5343\u6761\u968f\u673a\u7684\u4e03\u5341\u4e94\u78b1\u57fa\u5e8f\u5217<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">100%<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u9ed8\u8ba4\u6bd4\u8f83\u8303\u56f4\u5185\u4e5f\u6ca1\u6709\u76f8\u540c\u8005<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u4e24\u5343\u6761\u5168\u957f\u4e0d\u540c\u3001\u524d\u4e94\u5341\u78b1\u57fa\u76f8\u540c<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">0.05%<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u5171\u540c\u524d\u7f00\u5f62\u6210\u4e00\u4e2a\u7edf\u8ba1\u7c7b\u522b<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u540c\u4e00\u7ec4\u8bfb\u6bb5\uff0c\u660e\u786e\u6bd4\u8f83\u4e03\u5341\u4e94\u78b1\u57fa<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">100%<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u540e\u4e8c\u5341\u4e94\u4e2a\u78b1\u57fa\u533a\u5206\u5168\u90e8\u8bfb\u6bb5<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u4e00\u5343\u6761\u5b8c\u5168\u76f8\u540c\uff0c\u53e6\u6709\u4e00\u5343\u6761\u5404\u4e0d\u76f8\u540c<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">50.05%<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u53bb\u91cd\u540e\u5171\u4e00\u5343\u96f6\u4e00\u79cd<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<figure class=\"guide-figure\" style=\"margin:24px 0 28px\"><img loading=\"lazy\" decoding=\"async\" src=\"\/wordpress\/wp-content\/uploads\/2026\/11\/fastqc-duplication-prefix-50bp-versus-full-read-guide.png\" alt=\"\u4e24\u5343\u6761\u5168\u957f\u4e0d\u540c\uff0c\u4e3a\u4f55\u53ea\u5269\u4e00\u7c7b\u3002FastQC 0.12.1 \u5b9e\u8dd1\uff5c\u5bf9\u540c\u4e00\u4efd 75 \u78b1\u57fa\u957f\u5ea6\u7684\u8bfb\u6bb5\u6bd4\u8f83\u9ed8\u8ba4\u8bbe\u7f6e\u4e0e\u5168\u957f\u3002\u539f\u59cb\u8bfb\u6bb5\uff0c2000 \u6761\u5168\u957f\u4e0d\u540c\uff0c\u524d 50 \u4e2a\u78b1\u57fa\u76f8\u540c\uff0c\u540e 25 \u4e2a\u78b1\u57fa\u4e0d\u540c\u3002\u9ed8\u8ba4\u7edf\u8ba1\uff0c\u53bb\u91cd\u540e\u6bd4\u4f8b 0.05%\uff0c\u7528\u4e8e\u6bd4\u8f83\u7684\u5171\u540c\u524d\u7f00\u53ea\u6709\u4e00\u79cd\u3002\u660e\u786e\u6bd4\u5168\u957f\uff0c\u53bb\u91cd\u540e\u6bd4\u4f8b 100%\uff0c\u540c\u4e00\u4efd\u8f93\u5165\uff0c\u7528 75 \u4e2a\u78b1\u57fa\u533a\u5206\u5168\u90e8\u8bfb\u6bb5\u3002\" width=\"1200\" height=\"840\" style=\"display:block;width:100%;height:auto;border-radius:10px\"><figcaption style=\"font-size:14px;line-height:1.6;margin:9px 2px 0;color:#475569\">FastQC 0.12.1 \u5b9e\u8dd1\uff5c\u5bf9\u540c\u4e00\u4efd 75 \u78b1\u57fa\u957f\u5ea6\u7684\u8bfb\u6bb5\u6bd4\u8f83\u9ed8\u8ba4\u8bbe\u7f6e\u4e0e\u5168\u957f\u3002<\/figcaption><\/figure>\n<pre style=\"max-width:100%;box-sizing:border-box;overflow-x:auto;margin:18px 0;padding:14px 16px;background:#f3f6fa;color:#1a293c;border:1px solid #d8e1ea;border-radius:8px;line-height:1.55;white-space:pre;overflow-wrap:normal;word-break:normal\"><code class=\"language-bash\"># \u4e24\u4e2a\u76ee\u5f55\u5148\u5206\u522b\u521b\u5efa\uff0c\u4e24\u4e2a\u547d\u4ee4\u8bfb\u53d6\u540c\u4e00\u4efd\u957f\u5ea6\u5747\u4e3a 75 \u7684 FASTQ\r\nmkdir -p qc_default qc_full75\r\nfastqc --threads 1 -o qc_default common_prefix75.fastq\r\nfastqc --threads 1 --dup_length 75 -o qc_full75 common_prefix75.fastq\r\nunzip -p qc_default\/common_prefix75_fastqc.zip '*\/fastqc_data.txt' |\r\n  grep -A 3 'Sequence Duplication Levels'\r\n<\/code><\/pre>\n<p>\u672c\u6b21\u5b9e\u9645\u8fd0\u884c\u8fd8\u53d1\u73b0\u4e00\u4e2a\u7248\u672c\u7ec6\u8282\u3002\u9879\u76ee\u7f51\u7ad9\u7684\u5e2e\u52a9\u9875\u5199\u7684\u662f\u957f\u4e8e\u4e03\u5341\u4e94\u78b1\u57fa\u65f6\u622a\u53d6\u524d\u4e94\u5341\u4e2a\uff0c\u4f46\u5728 0.12.1 \u5bf9\u5e94\u7684\u5b9e\u73b0\u4e2d\uff0c\u9ed8\u8ba4\u6761\u4ef6\u662f\u5e8f\u5217\u957f\u4e8e\u4e94\u5341\u65f6\u622a\u5230\u4e94\u5341\u3002\u6211\u4eec\u53e6\u7528\u524d\u4e94\u5341\u76f8\u540c\u7684\u4e94\u5341\u4e00\u78b1\u57fa\u6587\u5e93\u505a\u5bf9\u7167\u3002\u5168\u957f\u56e0\u6700\u540e\u4e00\u4f4d\u4e0d\u540c\u5171\u6709\u56db\u79cd\uff0c\u9ed8\u8ba4\u62a5\u544a\u4ecd\u5c06\u5176\u5f52\u6210\u4e00\u4e2a\u7c7b\u522b\u3002\u628a\u76f8\u540c\u524d\u7f00\u5ef6\u957f\u4e3a\u4e03\u5341\u516d\u78b1\u57fa\u4e5f\u5f97\u5230\u76f8\u540c\u5224\u65ad\u3002\u9700\u8981\u5411\u540c\u884c\u89e3\u91ca\u4e00\u6b21\u7279\u5b9a\u8fd0\u884c\u65f6\uff0c\u5e94\u8bb0\u5f55\u5b9e\u9645\u7248\u672c\u548c\u6bd4\u8f83\u957f\u5ea6\uff0c\u9047\u5230\u4e0e\u7f51\u9875\u6458\u8981\u4e0d\u4e00\u81f4\u7684\u7ec6\u8282\uff0c\u518d\u7528\u4e00\u4e2a\u5df2\u77e5\u9633\u6027\u8f93\u5165\u590d\u6838\u3002<a style=\"color:#43619b;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/s-andrews\/FastQC\/blob\/v0.12.1\/uk\/ac\/babraham\/FastQC\/Modules\/OverRepresentedSeqs.java\">0.12.1 \u7248\u672c\u7684\u539f\u59cb\u5b9e\u73b0<\/a><\/p>\n<p>\u5982\u679c\u771f\u5b9e\u6587\u5e93\u7ecf\u8fc7\u957f\u5ea6\u4fee\u526a\uff0c\u5404\u6761\u8bfb\u957f\u4e0d\u7b49\uff0c\u5e94\u5148\u68c0\u67e5\u6700\u77ed\u8bfb\u6bb5\u548c\u957f\u5ea6\u5206\u5e03\uff0c\u907f\u514d\u7167\u6284\u8fd9\u91cc\u56fa\u5b9a\u4e3a\u4e03\u5341\u4e94\u7684\u8bbe\u7f6e\u3002\u4e5f\u53ef\u4ee5\u53e6\u5916\u505a\u4e00\u4efd\u6309\u957f\u5ea6\u5206\u5c42\u7684\u5c0f\u6837\u672c\uff0c\u4ec5\u5728\u76f8\u540c\u8bfb\u957f\u5185\u6bd4\u8f83\u524d\u7f00\u4e0e\u5168\u957f\uff0c\u5e76\u4fdd\u7559\u6bcf\u5c42\u5b9e\u9645\u62bd\u53d6\u7684\u8bfb\u6bb5\u6570\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u9047\u5230\u7ea2\u8272\u6a21\u5757\uff0c\u4e0b\u4e00\u6b65\u5e94\u8be5\u53d6\u54ea\u4e9b\u8bc1\u636e<\/h2>\n<p>\u5148\u53bb\u8fc7\u5ea6\u4ee3\u8868\u6a21\u5757\u6284\u4e0b\u771f\u5b9e\u88ab\u7edf\u8ba1\u7684\u5e8f\u5217\u548c\u957f\u5ea6\uff0c\u770b\u770b\u662f\u5426\u5bcc\u96c6\u5728\u56fa\u5b9a\u8bfb\u6bb5\u8d77\u70b9\u3001\u662f\u5426\u4e0e\u5df2\u77e5\u5f15\u7269\u548c\u63a5\u5934\u4e00\u81f4\uff0c\u518d\u4ece\u539f\u59cb FASTQ \u62bd\u53d6\u547d\u4e2d\u8be5\u524d\u7f00\u7684\u82e5\u5e72\u8bfb\u6bb5\uff0c\u6bd4\u8f83\u5b83\u4eec\u7684\u540e\u534a\u622a\u3002\u5982\u679c\u524d\u534a\u76f8\u540c\u3001\u540e\u534a\u9ad8\u5ea6\u591a\u6837\uff0c\u4f18\u5148\u67e5\u5efa\u5e93\u7ed3\u6784\u548c\u8bfb\u6bb5\u62c6\u5206\u3002\u5982\u679c\u5168\u957f\u4e5f\u4e00\u81f4\uff0c\u518d\u7ed3\u5408\u6bd4\u5bf9\u4f4d\u7f6e\u3001\u7247\u6bb5\u7aef\u70b9\u6216\u552f\u4e00\u5206\u5b50\u6807\u8bb0\uff0c\u8c03\u67e5\u662f\u771f\u5b9e\u9ad8\u4e30\u5ea6\u3001\u9776\u5411\u6355\u83b7\u8fd8\u662f\u6269\u589e\u52a0\u91cd\u3002\u5355\u4e00\u6a21\u5757\u65e0\u6cd5\u7ed9\u51fa\u8fd9\u51e0\u79cd\u539f\u56e0\u7684\u6bd4\u4f8b\u3002\u8f6f\u4ef6\u7684\u8fc7\u5ea6\u4ee3\u8868\u5e8f\u5217\u5e93\u4e5f\u53ea\u80fd\u63d0\u793a\u76f8\u4f3c\u6027\u548c\u5019\u9009\u6765\u6e90\uff0c\u4e0d\u80fd\u66ff\u4ee3\u5b9e\u9a8c\u8bbe\u8ba1\u8bb0\u5f55\u3002<a style=\"color:#43619b;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/Help\/3%20Analysis%20Modules\/9%20Overrepresented%20Sequences.html\">\u8fc7\u5ea6\u4ee3\u8868\u5e8f\u5217\u6a21\u5757\u7684\u68c0\u6d4b\u8303\u56f4\u4e0e\u9650\u5236<\/a><\/p>\n<p>\u4eba\u5de5\u5c0f\u6570\u636e\u8fd8\u6709\u4e00\u4e2a\u503c\u5f97\u7559\u5fc3\u7684\u5730\u65b9\u3002\u6211\u4eec\u6700\u521d\u628a\u6240\u6709\u8d28\u91cf\u5b57\u7b26\u90fd\u5199\u6210\u540c\u4e00\u4e2a\u9ad8\u4f4d\u5b57\u7b26\uff0cFastQC \u7684\u81ea\u52a8\u8bc6\u522b\u5c06\u5176\u62a5\u4f5c\u65e7\u7684 Illumina \u7f16\u7801\uff0c\u56e0\u800c\u8d28\u91cf\u9762\u677f\u51fa\u4e86\u610f\u6599\u4e4b\u5916\u7684\u8b66\u793a\u3002\u5728\u6700\u7ec8\u5c0f\u6d4b\u8bd5\u4e2d\uff0c\u6bcf\u4e00\u767e\u6761\u8bb0\u5f55\u7684\u6700\u540e\u4e00\u4f4d\u8bbe\u4e00\u4e2a\u4f4e\u8d28\u91cf\u5b57\u7b26\u4ee5\u63d0\u4f9b\u660e\u786e\u7684\u7f16\u7801\u8303\u56f4\uff0c\u62a5\u544a\u8bc6\u522b\u4e3a Sanger \u6216\u8f83\u65b0\u7684 Illumina\uff0c\u8d28\u91cf\u9762\u677f\u901a\u8fc7\u3002\u968f\u540e\u6240\u6709\u91cd\u590d\u5bf9\u7167\u90fd\u6cbf\u7528\u540c\u4e00\u8d28\u91cf\u5199\u6cd5\u3002\u771f\u5b9e\u7814\u7a76\u4e2d\u5e94\u67e5\u6d4b\u5e8f\u5e73\u53f0\u548c\u751f\u6210 FASTQ \u7684\u6d41\u7a0b\u6765\u5224\u65ad\u7f16\u7801\uff0c\u4e0d\u80fd\u901a\u8fc7\u4fee\u6539\u771f\u5b9e\u8d28\u91cf\u5b57\u7b26\u4e32\u8ba9\u9762\u677f\u53d8\u7eff\u3002\u8fd9\u4e2a\u7ecf\u5386\u8bf4\u660e\uff0c\u6a21\u62df\u6587\u4ef6\u4e5f\u9700\u8981\u72ec\u7acb\u68c0\u67e5\uff0c\u624d\u80fd\u786e\u4fe1\u6d4b\u8bd5\u7684\u786e\u662f\u9884\u8bbe\u7684\u4e00\u4e2a\u53d8\u91cf\u3002<\/p>\n<p class=\"guide-end\" style=\"margin-top:1.6em;padding-top:1.0em;border-top:1px solid #d7e0e9\">\u8981\u5c06\u8fd9\u5957\u68c0\u67e5\u7528\u4e8e\u4e00\u6279\u6587\u5e93\uff0c\u53ef\u5148\u56fa\u5b9a\u62bd\u53d6\u8bfb\u6bb5\u3001\u56de\u770b\u524d\u540e\u5e8f\u5217\u548c\u6838\u5bf9\u5efa\u5e93\u7ed3\u6784\u7684\u65b9\u6cd5\uff0c\u518d\u51b3\u5b9a\u54ea\u4e9b\u6837\u672c\u9700\u8981\u8fdb\u5165\u6bd4\u5bf9\u5c42\u9762\u590d\u6838\u3002\u9700\u8981\u96c6\u4e2d\u5206\u6790\u5927\u578b\u6d4b\u5e8f\u6279\u6b21\u65f6\uff0c\u53ef\u4e86\u89e3<a data-ysx-event=\"contact_click\" style=\"color:#43619b;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/www.biocloudservice.com\/wordpress\/compute-services\/shared-research-workspace\/\">\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668<\/a>\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u7528\u5b8c\u6574\u5e8f\u5217\u552f\u4e00\u3001\u5171\u4eab\u524d\u7f00\u4e0e\u771f\u5b9e\u91cd\u590d\u4e09\u79cd\u5bf9\u7167\u590d\u6838 FastQC 0.12.1\uff0c\u89e3\u91ca\u5e2e\u52a9\u9875\u4e0e\u7248\u672c\u5b9e\u73b0\u7684\u5dee\u5f02\u3002<\/p>\n","protected":false},"author":1,"featured_media":70028,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[527,530,953],"class_list":["post-70029","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-fastqc","tag-530","tag-953"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/fastqc-duplication-prefix-50bp-versus-full-read-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70029","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=70029"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70029\/revisions"}],"predecessor-version":[{"id":70030,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70029\/revisions\/70030"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/70028"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=70029"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=70029"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=70029"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}