{"id":70032,"date":"2026-11-14T22:53:53","date_gmt":"2026-11-14T14:53:53","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=70032"},"modified":"2026-11-14T22:53:53","modified_gmt":"2026-11-14T14:53:53","slug":"cellprofiler-ph3-parent-nuclei-border-counts","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/cellprofiler-ph3-parent-nuclei-border-counts\/","title":{"rendered":"CellProfiler \u5b9e\u8dd1\uff5c\u56fe\u91cc\u6709 22 \u4e2a PH3 \u5bf9\u8c61\uff0c\u4e3a\u4ec0\u4e48\u53ea\u6709 19 \u4e2a\u6838\u5e26\u4fe1\u53f7\uff1f"},"content":{"rendered":"
\n

\u7528\u663e\u5fae\u56fe\u50cf\u7b97\u9633\u6027\u7387\u65f6\uff0c\u5feb\u6377\u7684\u529e\u6cd5\u662f\u6253\u5f00\u56fe\u50cf\u6c47\u603b\u8868\uff0c\u62ff\u9633\u6027\u901a\u9053\u7684\u5bf9\u8c61\u6570\u9664\u4ee5\u6838\u6570\u3002\u6211\u4eec\u5728 CellProfiler \u4f5c\u8005\u63d0\u4f9b\u7684\u4eba\u7ed3\u80a0\u764c HT29 \u7ec6\u80de\u89c6\u91ce\u4e0a\u7167\u505a\uff0c\u5206\u5b50\u5f97\u5230\u4e8c\u5341\u4e8c\u3002\u56de\u5230\u9010\u6838\u6d4b\u91cf\u8868\uff0c\u7b26\u5408\u201c\u81f3\u5c11\u5e26\u4e00\u4e2a PH3 \u5bf9\u8c61\u201d\u7684\u6838\u53ea\u6709\u5341\u4e5d\u4e2a\u3002\u518d\u628a\u6838\u68c0\u6d4b\u7684\u4e00\u4e2a\u8fb9\u754c\u5f00\u5173\u7ffb\u8f6c\uff0c\u4e8c\u5341\u4e8c\u548c\u5341\u4e5d\u90fd\u6ca1\u53d8\uff0c\u6838\u7684\u603b\u6570\u5374\u4ece\u4e8c\u767e\u516b\u5341\u4e5d\u53d8\u6210\u4e09\u767e\u4e00\u5341\u516d\u3002\u8981\u51b3\u5b9a\u62a5\u544a\u54ea\u4e2a\u6bd4\u4f8b\uff0c\u5148\u5f97\u5f04\u6e05\u4e24\u4e2a\u901a\u9053\u8bc6\u522b\u4e86\u4ec0\u4e48\uff0c\u4ee5\u53ca\u4e00\u679a\u6838\u80fd\u4e0d\u80fd\u5bf9\u5e94\u4e24\u4e2a\u4fe1\u53f7\u5bf9\u8c61\u3002CellProfiler \u5b98\u65b9\u7684 Human cells \u793a\u4f8b\u4e0e\u56fe\u50cf<\/a><\/p>\n

\u987a\u7740\u4e09\u4e2a\u8367\u5149\u901a\u9053\u627e\u5230\u5bf9\u5e94\u7684\u6d4b\u91cf\u8868<\/h2>\n

\u516c\u5f00\u793a\u4f8b\u628a\u540c\u4e00\u89c6\u91ce\u5206\u6210\u4e09\u5f20\u7070\u5ea6\u56fe\u3002DNA \u901a\u9053\u7528\u6765\u5b9a\u4f4d\u7ec6\u80de\u6838\uff0cPH3 \u901a\u9053\u68c0\u6d4b\u78f7\u9178\u5316\u7ec4\u86cb\u767d H3 \u67d3\u8272\u5f62\u6210\u7684\u5bf9\u8c61\uff0c\u7ec6\u80de\u4f53\u901a\u9053\u63d0\u4f9b\u5411\u5916\u786e\u5b9a\u7ec6\u80de\u8f6e\u5ed3\u7684\u56fe\u50cf\u4fe1\u606f\u3002\u8fdb\u5165\u7ba1\u7ebf\u540e\uff0c\u4e24\u4e2a\u72ec\u7acb\u7684\u521d\u7ea7\u5bf9\u8c61\u8bc6\u522b\u6a21\u5757\u5206\u522b\u5f97\u5230\u6838\u4e0e PH3 \u5bf9\u8c61\uff0c\u518d\u7528\u5173\u7cfb\u6a21\u5757\u628a\u4fe1\u53f7\u5bf9\u8c61\u5173\u8054\u5230\u6838\u3002\u53e6\u4e00\u6761\u8def\u5f84\u4ee5\u6838\u4f5c\u79cd\u5b50\u8bc6\u522b\u7ec6\u80de\uff0c\u968f\u540e\u7528\u8f83\u5927\u7684\u7ec6\u80de\u548c\u8f83\u5c0f\u7684\u6838\u786e\u5b9a\u80de\u8d28\u3002\u60f3\u67e5\u9633\u6027\u6838\uff0c\u4f18\u5148\u770b\u6838\u4e0e\u4fe1\u53f7\u4e4b\u95f4\u7684\u7236\u5b50\u5173\u7cfb\u3002\u60f3\u67e5\u80de\u8d28\u5f3a\u5ea6\uff0c\u5219\u53bb\u627e\u4e0e\u8fd9\u679a\u6838\u5bf9\u5e94\u7684\u7ec6\u80de\u548c\u80de\u8d28\u5bf9\u8c61\u3002\u628a\u4e24\u4e2a\u4efb\u52a1\u653e\u5728\u540c\u4e00\u5f20\u56fe\u4e0a\uff0c\u5e76\u4e0d\u610f\u5473\u7740\u5b83\u4eec\u7684\u8ba1\u6570\u5355\u4f4d\u76f8\u540c\u3002<\/p>\n

\u56fe\u50cf\u8868\u6bcf\u4e2a\u89c6\u91ce\u4e00\u884c\uff0c\u9002\u5408\u53d1\u73b0\u6f0f\u901a\u9053\u6216\u5f02\u5e38\u8ba1\u6570\u3002\u6838\u8868\u6bcf\u679a\u6838\u4e00\u884c\uff0c\u80fd\u8bfb\u51fa\u5b83\u6709\u591a\u5c11\u4e2a PH3 \u5b50\u5bf9\u8c61\u3002PH3 \u8868\u6bcf\u4e2a\u4fe1\u53f7\u5bf9\u8c61\u4e00\u884c\uff0c\u53ef\u4ee5\u53cd\u5411\u68c0\u67e5\u5b83\u5f52\u5c5e\u4e8e\u54ea\u679a\u6838\u3002\u4e09\u5f20\u8868\u7684\u5bf9\u8c61\u7f16\u53f7\u5404\u6709\u81ea\u5df1\u7684\u8303\u56f4\u3002\u5408\u5e76\u6574\u677f\u6570\u636e\u65f6\uff0c\u5982\u679c\u53ea\u6309\u5bf9\u8c61\u7f16\u53f7\u8fde\u63a5\uff0c\u4e0b\u4e00\u5f20\u56fe\u7684\u7b2c\u4e00\u679a\u6838\u5c31\u53ef\u80fd\u914d\u5230\u4e0a\u4e00\u5f20\u56fe\u7684\u7b2c\u4e00\u4e2a\u4fe1\u53f7\u3002\u5148\u5e26\u4e0a\u5b9e\u9a8c\u548c\u89c6\u91ce\u6807\u8bc6\uff0c\u786e\u8ba4\u56fe\u50cf\u7f16\u53f7\uff0c\u518d\u6309\u7236\u6838\u7f16\u53f7\u8fde\u63a5\u3002\u8fd9\u9879\u68c0\u67e5\u6bd4\u5728\u6700\u540e\u4e00\u5217\u8c03\u6574\u767e\u5206\u6570\u66f4\u5bb9\u6613\u53d1\u73b0\u8bef\u914d\u3002CellProfiler \u5bf9\u8c61\u5904\u7406\u4e0e\u7236\u5b50\u5173\u7cfb\u6587\u6863<\/a><\/p>\n

\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u628a\u4e8c\u5341\u4e8c\u4e2a\u4fe1\u53f7\u8fd8\u539f\u5230\u5341\u4e5d\u679a\u6838<\/h2>\n

\u5b89\u88c5 CellProfiler 4.2.8 \u540e\uff0c\u6211\u4eec\u5b8c\u6574\u6267\u884c\u4f5c\u8005\u7684\u5341\u56db\u6a21\u5757\u7ba1\u7ebf\uff0c\u8f93\u5165\u662f\u538b\u7f29\u5305\u91cc\u7684\u4e09\u5f20\u539f\u59cb TIFF\u3002\u7b2c\u4e00\u6b21\u4fdd\u7559\u793a\u4f8b\u8bbe\u7f6e\u3002\u7b2c\u4e8c\u6b21\u53ea\u628a DNA \u6838\u8bc6\u522b\u6a21\u5757\u4e2d\u201c\u4e22\u5f03\u89e6\u53ca\u56fe\u50cf\u8fb9\u7f18\u7684\u5bf9\u8c61\u201d\u6539\u4e3a\u5426\u3002PH3 \u8bc6\u522b\u6a21\u5757\u4e5f\u6709\u540c\u540d\u5f00\u5173\uff0c\u4ecd\u7528\u539f\u503c\u3002\u4e0b\u9762\u662f\u4ece\u8f93\u51fa\u8868\u8bfb\u53d6\u7684\u5b9e\u9645\u884c\u6570\u3002\u6bcf\u6b21\u53e6\u5b58\u8f93\u51fa\u76ee\u5f55\uff0c\u9632\u6b62\u540c\u540d\u56fe\u50cf\u8868\u4e92\u76f8\u8986\u76d6\u3002\u60f3\u590d\u73b0\u65f6\uff0c\u5148\u786e\u8ba4\u4e09\u5f20\u8f93\u5165\u7684\u901a\u9053\u540e\u7f00\u5206\u522b\u843d\u5728 DNA\u3001PH3\u3001\u7ec6\u80de\u4f53\u8fd9\u4e09\u680f\uff0c\u518d\u505a\u53c2\u6570\u6bd4\u8f83\u3002\u5b9e\u9a8c\u6240\u7528\u7684\u5b98\u65b9\u793a\u4f8b\u538b\u7f29\u5305<\/a><\/p>\n

\n\n\n\n\n\n\n\n\n
\u540c\u4e00\u89c6\u91ce\u7684\u8bfb\u6570<\/th>\n\u9ed8\u8ba4\u6392\u9664\u8d34\u8fb9\u6838<\/th>\n\u4fdd\u7559\u8d34\u8fb9\u6838<\/th>\n<\/tr>\n<\/thead>\n
\u6838\u3001\u7ec6\u80de\u3001\u80de\u8d28\u5bf9\u8c61\u6570<\/td>\n\u5404 289<\/td>\n\u5404 316<\/td>\n<\/tr>\n
PH3 \u5bf9\u8c61\u6570<\/td>\n22<\/td>\n22<\/td>\n<\/tr>\n
\u5e26 0\u30011\u30012 \u4e2a PH3 \u5bf9\u8c61\u7684\u6838<\/td>\n270\u300116\u30013<\/td>\n297\u300116\u30013<\/td>\n<\/tr>\n
\u81f3\u5c11\u5e26\u4e00\u4e2a PH3 \u7684\u6838\u5360\u6bd4<\/td>\n19\/289\uff0c6.57%<\/td>\n19\/316\uff0c6.01%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"22
\u540c\u4e00\u5f20 HT29 \u7ec6\u80de\u56fe\u50cf\uff0c\u5148\u8bfb\u7236\u5b50\u5173\u7cfb\uff0c\u518d\u6838\u7b97\u9633\u6027\u7387\u3002<\/figcaption><\/figure>\n

\u4e24\u6b21\u8fd0\u884c\u90fd\u6709\u5341\u516d\u679a\u6838\u5404\u5e26\u4e00\u4e2a\u4fe1\u53f7\uff0c\u4e09\u679a\u6838\u5404\u5e26\u4e24\u4e2a\u3002\u6309\u5bf9\u8c61\u76f8\u52a0\u662f\u5341\u516d\u52a0\u4e8c\u4e58\u4e09\uff0c\u5f97\u5230\u4e8c\u5341\u4e8c\u4e2a\u4fe1\u53f7\u3002\u6309\u6709\u4fe1\u53f7\u7684\u6838\u76f8\u52a0\u662f\u5341\u516d\u52a0\u4e09\uff0c\u5f97\u5230\u5341\u4e5d\u679a\u6838\u3002\u7ffb\u5f00 PH3 \u8868\uff0c\u4e8c\u5341\u4e8c\u884c\u5168\u90e8\u6709\u7236\u6838\uff0c\u6070\u597d\u6709\u5341\u4e5d\u4e2a\u4e0d\u540c\u7684\u7236\u6838\u7f16\u53f7\u3002\u8fd9\u5c31\u6392\u9664\u4e86\u4fe1\u53f7\u5bf9\u8c61\u672a\u5206\u914d\u9020\u6210\u4e09\u4e2a\u4e4b\u5dee\u7684\u89e3\u91ca\u3002\u82e5\u7528\u4e8c\u5341\u4e8c\u9664\u4ee5\u4e8c\u767e\u516b\u5341\u4e5d\uff0c\u5f97\u5230\u7ea6\u96f6\u70b9\u96f6\u4e03\u516d\u4e2a\u4fe1\u53f7\u5bf9\u8c61\u6bcf\u679a\u6838\u3002\u628a\u5b83\u5199\u6210\u767e\u5206\u4e4b\u4e03\u70b9\u516d\u4e00\u7684\u9633\u6027\u6838\uff0c\u4f1a\u91cd\u590d\u8ba1\u5165\u90a3\u4e09\u679a\u5404\u6709\u4e24\u4e2a\u4fe1\u53f7\u7684\u6838\u3002<\/p>\n

import csv\r\nfrom collections import Counter\r\n\r\nwith open("Nuclei.csv", newline="", encoding="utf-8-sig") as stream:\r\n    nuclei = list(csv.DictReader(stream))\r\nhist = Counter(int(row["Children_PH3_Count"]) for row in nuclei)\r\nwith_signal = sum(n for k, n in hist.items() if k > 0)\r\nprint(sorted(hist.items()), with_signal, len(nuclei))\r\n<\/code><\/pre>\n
\u54ea\u4e9b\u5b9e\u9a8c\u9002\u5408\u6392\u9664\u8d34\u8fb9\u6838<\/h2>\n
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\u672c\u4f8b\u7684\u6838\u3001\u7ec6\u80de\u3001\u80de\u8d28\u8ba1\u6570\u540c\u589e\u4e8c\u5341\u4e03\uff0c\u662f\u56e0\u4e3a\u8be5\u89c6\u91ce\u7684\u540e\u7eed\u6a21\u5757\u4f9d\u8d56\u6838\u4f5c\u4e3a\u79cd\u5b50\uff0c\u4e14\u672c\u6b21\u8f93\u51fa\u6070\u597d\u4fdd\u6301\u4e86\u8fd9\u6837\u7684\u4e00\u4e00\u8ba1\u6570\u3002\u771f\u5b9e\u56fe\u50cf\u4e2d\uff0c\u7ec6\u80de\u8f6e\u5ed3\u89e6\u8fb9\u3001\u6838\u5206\u88c2\u8fc7\u5ea6\u3001\u4fe1\u53f7\u5f3a\u5ea6\u4e0d\u8db3\u90fd\u53ef\u80fd\u6539\u53d8\u8fd9\u79cd\u5173\u7cfb\u3002\u6709\u5341\u4e5d\u679a\u6838\u5173\u8054\u5230\u4fe1\u53f7\uff0c\u4e5f\u53ea\u652f\u6301\u6309\u8fd9\u5957\u68c0\u6d4b\u89c4\u5219\u5b9a\u4e49\u7684\u5341\u4e5d\u679a\u3002\u82e5\u8981\u628a\u5b83\u89e3\u91ca\u4e3a\u6709\u4e1d\u5206\u88c2\u7ec6\u80de\uff0c\u5e94\u67e5\u770b\u6838\u5f62\u6001\u3001\u4fe1\u53f7\u7684\u5b9a\u4f4d\u548c\u5bf9\u7167\u67d3\u8272\uff0c\u9a8c\u8bc1\u8bc6\u522b\u51fa\u7684\u5f31\u4fe1\u53f7\u6216\u788e\u7247\u662f\u5426\u7b26\u5408\u5b9e\u9a8c\u5b9a\u4e49\u3002\u5355\u4e2a\u516c\u5f00\u89c6\u91ce\u53ef\u7528\u6765\u7ec3\u4e60\u6838\u7b97\uff0c\u8fd8\u4e0d\u8db3\u4ee5\u4f30\u8ba1\u4e0d\u540c\u5904\u7406\u7ec4\u7684\u751f\u7269\u5b66\u5dee\u5f02\u3002<\/p>\n
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