{"id":70116,"date":"2026-11-14T23:16:43","date_gmt":"2026-11-14T15:16:43","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=70116"},"modified":"2026-11-14T23:16:43","modified_gmt":"2026-11-14T15:16:43","slug":"manta-breakend-pairs-mateid-split-read-evidence","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/manta-breakend-pairs-mateid-split-read-evidence\/","title":{"rendered":"Manta \u5b9e\u8dd1\uff5c\u4f53\u7ec6\u80de VCF \u6709 6 \u884c\u65ad\u7aef\uff0c\u600e\u6837\u8fd8\u539f\u6210 3 \u5bf9\u76f8\u8fde\u7684\u4f4d\u7f6e\uff1f"},"content":{"rendered":"<div class=\"ysx-method-guide\" style=\"max-width:820px;min-width:0;margin:0 auto;color:#26374a;line-height:1.86;overflow-wrap:anywhere\">\n<p>\u7528\u77ed\u8bfb\u957f\u6570\u636e\u68c0\u6d4b\u7ed3\u6784\u53d8\u5f02\uff0c\u5e38\u4f1a\u770b\u5230\u66ff\u4ee3\u7b49\u4f4d\u91cc\u51fa\u73b0\u67d3\u8272\u4f53\u540d\u3001\u5750\u6807\u548c\u65b9\u62ec\u53f7\u3002\u628a\u7b5b\u9009\u540e\u7684\u8868\u6309\u884c\u6570\u6c47\u603b\uff0c\u5f88\u5bb9\u6613\u628a\u65ad\u7aef\u6570\u62a5\u6210\u4e8b\u4ef6\u6570\u3002\u6211\u4eec\u8fd0\u884c\u4e86 Manta \u7684\u80bf\u7624\u4e0e\u6b63\u5e38\u914d\u5bf9\u5b98\u65b9\u793a\u4f8b\uff0c\u4f53\u7ec6\u80de\u7ed3\u679c\u6070\u597d\u662f\u516d\u884c\uff0c\u5168\u90e8\u901a\u8fc7\u8fc7\u6ee4\u3002\u6cbf\u7740\u6bcf\u884c\u7684\u4f19\u4f34\u7f16\u53f7\u53cd\u67e5\uff0c\u516d\u884c\u53ea\u6784\u6210\u4e09\u5bf9\u8fde\u63a5\u3002\u5176\u4e2d\u4e00\u5bf9\u8fd8\u80fd\u770b\u5230\u4e24\u7aef\u5750\u6807\u5728\u5b57\u7b26\u4e32\u4e2d\u76f8\u5dee\u4e24\u4e2a\u78b1\u57fa\uff0c\u53e6\u4e00\u5bf9\u751a\u81f3\u6ca1\u6709\u88c2\u5206\u8bfb\u6bb5\u5b57\u6bb5\u3002\u8fd9\u4e9b\u7ec6\u8282\u51b3\u5b9a\u4e86\u80fd\u5426\u51c6\u786e\u5730\u8bf4\u51fa\u65ad\u70b9\u7684\u4f4d\u7f6e\u4ee5\u53ca\u8bc1\u636e\u5f3a\u5ea6\u3002<\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5148\u51b3\u5b9a\u8981\u6570\u7684\u662f\u7aef\u70b9\u3001\u8fde\u63a5\u8fd8\u662f\u590d\u6742\u4e8b\u4ef6<\/h2>\n<p>VCF \u7684\u65ad\u7aef\u8bb0\u5f55\u53ea\u4ece\u8fde\u63a5\u7684\u4e00\u4fa7\u5199\u8d77\u3002\u66ff\u4ee3\u7b49\u4f4d\u4e2d\u7684\u8fdc\u7aef\u5750\u6807\u548c\u62ec\u53f7\u65b9\u5411\u8bf4\u660e\u5b83\u600e\u6837\u63a5\u5411\u53e6\u4e00\u6bb5\u5e8f\u5217\uff0c\u8bb0\u5f55\u6807\u8bc6\u4e0e\u4f19\u4f34\u6807\u8bc6\u5219\u5e2e\u52a9\u627e\u5230\u53e6\u4e00\u4fa7\u5bf9\u8be5\u8fde\u63a5\u7684\u63cf\u8ff0\u3002\u60f3\u8ba1\u7b97\u5df2\u914d\u5bf9\u65ad\u7aef\u6570\uff0c\u53ef\u4ee5\u6309\u6807\u8bc6\u904d\u5386\u5e76\u8981\u6c42\u53cd\u5411\u6307\u56de\u3002\u60f3\u7edf\u8ba1\u7ed3\u6784\u8fde\u63a5\u6570\uff0c\u4e92\u6307\u7684\u4e00\u5bf9\u53ea\u5e94\u8d21\u732e\u4e00\u6b21\u3002\u82e5\u7814\u7a76\u7684\u662f\u590d\u6742\u91cd\u6392\uff0c\u4e00\u4e2a\u751f\u7269\u5b66\u4e8b\u4ef6\u53ef\u80fd\u5305\u542b\u591a\u6761\u8fde\u63a5\uff0c\u56e0\u6b64\u628a\u516d\u884c\u8fd8\u539f\u6210\u4e09\u5bf9\u4e4b\u540e\uff0c\u8fd8\u8981\u53e6\u627e\u8bc1\u636e\u5224\u65ad\u8fd9\u4e09\u5bf9\u80fd\u5426\u5f52\u5165\u540c\u4e00\u4e8b\u4ef6\u3002<a style=\"color:#226985;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/samtools.github.io\/hts-specs\/VCFv4.3.pdf\">VCF \u89c4\u8303\u4e2d\u7684\u65ad\u7aef\u4e0e\u7ed3\u6784\u53d8\u5f02\u8868\u793a<\/a><\/p>\n<p>Manta \u8f93\u51fa\u7684\u4e0d\u540c\u6587\u4ef6\u670d\u52a1\u4e8e\u4e0d\u540c\u7528\u9014\u3002\u5019\u9009\u6587\u4ef6\u8bb0\u5f55\u8fdb\u5165\u540e\u7eed\u8bc4\u5206\u7684\u5019\u9009\uff0c\u4f53\u7ec6\u80de\u6587\u4ef6\u9488\u5bf9\u80bf\u7624\u4e0e\u914d\u5bf9\u6b63\u5e38\u6a21\u5f0f\u7ed9\u51fa\u8bc4\u5206\u53ca\u6837\u672c\u652f\u6301\u3002\u5019\u9009\u884c\u7684\u8fc7\u6ee4\u503c\u4e3a\u70b9\uff0c\u901a\u5e38\u8868\u793a\u8be5\u5b57\u6bb5\u672a\u7ed9\u51fa\u8fc7\u6ee4\u5224\u5b9a\uff0c\u4e0d\u80fd\u5199\u6210\u5df2\u7ecf\u901a\u8fc7\u3002\u628a\u5019\u9009\u96c6\u4e0e\u4f53\u7ec6\u80de\u96c6\u62fc\u5728\u4e00\u8d77\u6309\u884c\u7edf\u8ba1\uff0c\u4f1a\u628a\u540c\u4e00\u6761\u8fde\u63a5\u7684\u53d1\u73b0\u8bb0\u5f55\u548c\u8bc4\u5206\u7ed3\u679c\u90fd\u8ba1\u5165\u3002\u8bfb\u8005\u5e94\u5148\u786e\u5b9a\u8981\u8bc4\u4f30\u54ea\u4e00\u79cd\u8f93\u51fa\uff0c\u518d\u4fdd\u7559\u539f\u59cb\u6807\u8bc6\uff0c\u4ee5\u4fbf\u56de\u67e5\u524d\u4e00\u9636\u6bb5\u3002<a style=\"color:#226985;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/Illumina\/manta\/blob\/v1.6.0\/docs\/userGuide\/README.md\">Manta \u7528\u6237\u624b\u518c\u4e2d\u7684\u7ed3\u679c\u6587\u4ef6\u8bf4\u660e<\/a><\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u516d\u884c\u7684\u4f19\u4f34\u5173\u7cfb\u5982\u4f55\u4e92\u76f8\u9a8c\u7b97<\/h2>\n<p>\u6211\u4eec\u5b89\u88c5 Manta 1.6.0\uff0c\u7528\u53d1\u884c\u5305\u63d0\u4f9b\u7684 HCC1954 \u80bf\u7624\u53ca\u914d\u5bf9\u6b63\u5e38\u6bd4\u5bf9\u3001\u5c40\u90e8\u53c2\u8003\u548c\u6f14\u793a\u811a\u672c\u5b9e\u9645\u8dd1\u5b8c\u5019\u9009\u53d1\u73b0\u3001\u8bc4\u5206\u548c\u793a\u4f8b\u81ea\u68c0\u3002\u7ed3\u679c\u4e0e\u968f\u5305\u9884\u671f\u6587\u4ef6\u6309\u793a\u4f8b\u89c4\u5219\u6bd4\u8f83\u901a\u8fc7\u3002\u5019\u9009\u7ed3\u6784\u53d8\u5f02\u6587\u4ef6\u6709\u516d\u6761\uff0c\u4f53\u7ec6\u80de\u6587\u4ef6\u4e5f\u662f\u516d\u6761\uff0c\u5168\u90e8\u5c5e\u4e8e\u65ad\u7aef\u7c7b\u578b\u3002\u9010\u4e00\u8981\u6c42\u4e00\u4fa7\u7684\u4f19\u4f34\u7f16\u53f7\u5728\u540c\u4e00\u6587\u4ef6\u4e2d\u5b58\u5728\uff0c\u4e14\u53e6\u4e00\u4fa7\u7684\u4f19\u4f34\u7f16\u53f7\u6b63\u597d\u6307\u56de\uff0c\u4e24\u4e2a\u6587\u4ef6\u90fd\u5f97\u5230\u4e09\u5bf9\u3002\u5c0f\u63d2\u5165\u7f3a\u5931\u5019\u9009\u6587\u4ef6\u548c\u4e8c\u500d\u4f53\u7ed3\u6784\u53d8\u5f02\u6587\u4ef6\u5404\u6709\u6587\u4ef6\u5934\uff0c\u6570\u636e\u8bb0\u5f55\u90fd\u662f\u96f6\u6761\u3002\u8fd9\u91cc\u68c0\u9a8c\u7684\u662f\u6559\u7a0b\u6240\u7528\u7684\u5c0f\u8303\u56f4\uff0c\u4e0d\u53cd\u6620\u6574\u4e2a\u6837\u672c\u7684\u53d8\u5f02\u8d1f\u62c5\u3002<a style=\"color:#226985;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/Illumina\/manta\/releases\/tag\/v1.6.0\">Manta \u5b98\u65b9\u53d1\u884c\u5305<\/a><\/p>\n<div class=\"guide-table\" style=\"max-width:100%;overflow-x:auto;margin:18px 0\">\n<table style=\"display:table;width:100%;max-width:100%;min-width:0;table-layout:fixed;border-collapse:collapse;font-size:.92em;line-height:1.6\">\n<thead>\n<tr>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eafaef;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u7b2c\u4e00\u4fa7\u4e0e\u7b2c\u4e8c\u4fa7\u7684\u4f4d\u7f6e<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eafaef;color:#1e293b;vertical-align:top;text-align:right;padding:6px;border:1px solid #c4cfdb\">\u80bf\u7624\u66ff\u4ee3\u652f\u6301\u7684\u8bfb\u5bf9<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eafaef;color:#1e293b;vertical-align:top;text-align:right;padding:6px;border:1px solid #c4cfdb\">\u80bf\u7624\u66ff\u4ee3\u652f\u6301\u7684\u88c2\u5206\u8bfb\u6bb5<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#eafaef;color:#1e293b;vertical-align:top;text-align:right;padding:6px;border:1px solid #c4cfdb\">\u4f53\u7ec6\u80de\u8bc4\u5206<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">8 \u7684 107653411 \u4e0e 11 \u7684 94987872<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">4<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">19<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">35<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">8 \u7684 107653518 \u4e0e 11 \u7684 94975747<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">9<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">15<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">36<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">11 \u7684 94975753 \u4e0e 11 \u7684 94987865<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">13<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">\u65e0\u8be5\u5b57\u6bb5<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb;text-align:right\">41<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<figure class=\"guide-figure\" style=\"margin:24px 0 28px\"><img loading=\"lazy\" decoding=\"async\" src=\"\/wordpress\/wp-content\/uploads\/2026\/11\/manta-breakend-pairs-mateid-split-read-evidence-guide.png\" alt=\"\u516d\u6761\u65ad\u7aef\uff0c\u4e09\u5bf9\u8fde\u63a5\u3002\u4e92\u67e5\u6bcf\u6761 MATEID\uff0c\u518d\u6838\u5bf9\u65ad\u70b9\u533a\u95f4\u53ca\u80bf\u7624\u4e0e\u6b63\u5e38\u7684\u8bfb\u6bb5\u652f\u6301\u3002\u770b\u4f19\u4f34\uff0c6 \u884c \u2192 3 \u5bf9\uff0c\u4e24\u7aef\u53cd\u5411\u6307\u56de\uff0c\u4e00\u6b21\u53ea\u8ba1\u4e00\u6761\u8fde\u63a5\u3002\u770b\u540c\u6e90\uff0c2 bp \u504f\u79fb\uff0c\u4e00\u5bf9\u65ad\u7aef\u542b\u4e24\u78b1\u57fa\u5fae\u540c\u6e90\uff0c\u8fdc\u7aef\u5750\u6807\u6709\u4f59\u5730\u3002\u770b\u7f6e\u4fe1\u533a\u95f4\uff0c\u00b1156 bp\uff0c\u4e00\u6761\u6709 13 \u5bf9\u66ff\u4ee3\u652f\u6301\u7684\u8fde\u63a5\u672a\u62a5\u544a SR\u3002\" width=\"1200\" height=\"840\" style=\"display:block;width:100%;height:auto;border-radius:10px\"><figcaption style=\"font-size:14px;line-height:1.6;margin:9px 2px 0;color:#475569\">\u4e92\u67e5\u6bcf\u6761 MATEID\uff0c\u518d\u6838\u5bf9\u65ad\u70b9\u533a\u95f4\u53ca\u80bf\u7624\u4e0e\u6b63\u5e38\u7684\u8bfb\u6bb5\u652f\u6301\u3002<\/figcaption><\/figure>\n<p>\u7b2c\u4e00\u5bf9\u4e2d\u7684\u516b\u53f7\u67d3\u8272\u4f53\u8bb0\u5f55\u5199\u6709\u4f19\u4f34\u5341\u4e00\u53f7\u67d3\u8272\u4f53\u7684\u5750\u6807\uff0c\u53e6\u4e00\u4fa7\u4e5f\u80fd\u6307\u56de\u516b\u53f7\u3002\u80bf\u7624\u6837\u672c\u7684\u8bfb\u5bf9\u5b57\u6bb5\u662f\u56db\u767e\u516b\u5341\u4e5d\u548c\u56db\uff0c\u88c2\u5206\u8bfb\u6bb5\u5b57\u6bb5\u662f\u4e94\u767e\u4e8c\u5341\u548c\u5341\u4e5d\u3002\u5404\u81ea\u7b2c\u4e00\u9879\u662f\u652f\u6301\u53c2\u8003\u7684\u6570\uff0c\u7b2c\u4e8c\u9879\u662f\u652f\u6301\u66ff\u4ee3\u8fde\u63a5\u7684\u6570\u3002\u6b63\u5e38\u6837\u672c\u5728\u4e24\u79cd\u8bc1\u636e\u4e0a\u5206\u522b\u4e3a\u4e09\u5341\u4e8c\u548c\u96f6\u3001\u4e8c\u5341\u516b\u548c\u96f6\u3002\u672c\u6b21\u4e24\u7aef\u628a\u540c\u4e00\u7ec4\u6570\u5e26\u4e86\u4e24\u904d\uff0c\u4e0d\u80fd\u628a\u56db\u52a0\u56db\u62a5\u6210\u516b\u5bf9\u80bf\u7624\u652f\u6301\uff0c\u4e5f\u4e0d\u80fd\u628a\u5341\u4e5d\u52a0\u5341\u4e5d\u5f53\u6210\u4e09\u5341\u516b\u6761\u72ec\u7acb\u88c2\u5206\u8bc1\u636e\u3002\u82e5\u5173\u5fc3\u72ec\u7acb\u5206\u5b50\u6570\uff0c\u8fd8\u8981\u56de\u67e5\u539f\u59cb\u8bfb\u6bb5\u548c\u6a21\u677f\u5173\u7cfb\uff0c\u5355\u51ed\u8fd9\u4e24\u5217\u65e0\u6cd5\u63a8\u51fa\u3002<\/p>\n<p>\u7b2c\u4e8c\u5bf9\u6700\u5bb9\u6613\u8ba9\u5750\u6807\u5339\u914d\u811a\u672c\u51fa\u9519\u3002\u516b\u53f7\u4e00\u4fa7\u5b57\u7b26\u4e32\u91cc\u7684\u8fdc\u7aef\u5750\u6807\u662f\u5341\u4e00\u53f7\u7684\u4e5d\u5343\u56db\u767e\u4e5d\u5341\u4e03\u4e07\u4e94\u5343\u4e03\u767e\u56db\u5341\u4e5d\uff0c\u4f19\u4f34\u8bb0\u5f55\u81ea\u5df1\u7684\u8d77\u70b9\u5374\u662f\u4e5d\u5343\u56db\u767e\u4e5d\u5341\u4e03\u4e07\u4e94\u5343\u4e03\u767e\u56db\u5341\u4e03\u3002\u4e24\u4fa7\u90fd\u7ed9\u51fa\u96f6\u5230\u4e8c\u7684\u5750\u6807\u504f\u79fb\u533a\u95f4\uff0c\u4ee5\u53ca\u957f\u4e24\u4e2a\u78b1\u57fa\u7684\u540c\u6e90\u5e8f\u5217\u3002\u77ed\u540c\u6e90\u4f7f\u7b49\u4ef7\u7684\u65ad\u70b9\u4f4d\u7f6e\u5b58\u5728\u4f59\u5730\u3002\u82e5\u5f3a\u5236\u8981\u6c42\u8fdc\u7aef\u5750\u6807\u7b49\u4e8e\u4f19\u4f34\u884c\u8d77\u70b9\uff0c\u660e\u660e\u4e92\u6307\u7684\u8bb0\u5f55\u5c31\u4f1a\u6f0f\u914d\u3002\u5148\u4ee5\u7f16\u53f7\u4e92\u6307\u5efa\u7acb\u5173\u8054\uff0c\u518d\u7ed3\u5408\u65b9\u5411\u3001\u540c\u6e90\u5e8f\u5217\u548c\u533a\u95f4\u6821\u9a8c\u4f4d\u7f6e\uff0c\u80fd\u4fdd\u7559\u8fd9\u4e00\u5c42\u4fe1\u606f\u3002<\/p>\n<pre style=\"max-width:100%;box-sizing:border-box;overflow-x:auto;margin:18px 0;padding:14px 16px;background:#f3f6fa;color:#1a293c;border:1px solid #d8e1ea;border-radius:8px;line-height:1.55;white-space:pre;overflow-wrap:normal;word-break:normal\"><code class=\"language-python\">pairs = set()\r\nfor key, record in records_by_id.items():\r\n    mate = record.get(&quot;MATEID&quot;)\r\n    if mate in records_by_id and records_by_id[mate].get(&quot;MATEID&quot;) == key:\r\n        pairs.add(tuple(sorted((key, mate))))\r\nprint(len(pairs))\r\n<\/code><\/pre>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u600e\u6837\u5411\u4e0b\u6e38\u4ea4\u4ed8\u4e00\u4e2a\u53ef\u6838\u67e5\u7684\u65ad\u70b9<\/h2>\n<p>\u7b2c\u4e09\u5bf9\u7684\u4e24\u4fa7\u90fd\u843d\u5728\u5341\u4e00\u53f7\u67d3\u8272\u4f53\u3002\u80bf\u7624\u4e2d\u6709\u5341\u4e09\u5bf9\u8bfb\u6bb5\u652f\u6301\u66ff\u4ee3\u8fde\u63a5\uff0c\u6b63\u5e38\u6837\u672c\u8be5\u9879\u4e3a\u96f6\u3002\u4e24\u7aef\u7ed9\u51fa\u7684\u5750\u6807\u8303\u56f4\u5206\u522b\u76f8\u5bf9\u8d77\u70b9\u524d\u540e\u4e00\u767e\u4e94\u5341\u516d\u548c\u4e00\u767e\u4e94\u5341\u4e2a\u78b1\u57fa\u3002\u6b64\u884c\u683c\u5f0f\u4e2d\u538b\u6839\u6ca1\u6709\u88c2\u5206\u8bfb\u6bb5\u5b57\u6bb5\uff0c\u5e94\u5199\u4f5c\u201c\u672a\u62a5\u544a\u201d\uff0c\u4e0d\u80fd\u586b\u6210\u5b9e\u6d4b\u96f6\u6761\u3002\u5b83\u7684\u4f53\u7ec6\u80de\u8bc4\u5206\u8fd8\u662f\u4e09\u5bf9\u4e2d\u6700\u9ad8\u7684\u56db\u5341\u4e00\uff0c\u8bf4\u660e\u9ad8\u8bc4\u5206\u4e0e\u78b1\u57fa\u7ea7\u5b9a\u4f4d\u7cbe\u5ea6\u4e0d\u662f\u540c\u4e00\u4e2a\u6307\u6807\u3002\u7ed9\u540e\u7eed\u5b9e\u9a8c\u8bbe\u8ba1\u5f15\u7269\u65f6\uff0c\u8981\u4fdd\u7559\u6574\u4e2a\u533a\u95f4\u548c\u65b9\u5411\uff0c\u4e0d\u80fd\u9760\u8bc4\u5206\u628a\u4e00\u4e2a\u8f83\u5bbd\u7684\u65ad\u70b9\u8303\u56f4\u7f29\u6210\u5355\u4e00\u78b1\u57fa\u3002<a style=\"color:#226985;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/raw.githubusercontent.com\/Illumina\/manta\/v1.6.0\/docs\/userGuide\/README.md\">Manta \u5bf9\u540c\u6e90\u3001\u7f6e\u4fe1\u533a\u95f4\u548c\u652f\u6301\u5b57\u6bb5\u7684\u5b9a\u4e49<\/a><\/p>\n<p>\u505a\u8de8\u6837\u672c\u6bd4\u8f83\u65f6\uff0c\u6807\u8bc6\u7f16\u53f7\u9002\u5408\u540c\u4e00\u6b21\u8c03\u7528\u5185\u90e8\u914d\u5bf9\uff0c\u4e0d\u80fd\u5047\u8bbe\u76f8\u540c\u7f16\u53f7\u5728\u53e6\u4e00\u4e2a\u6837\u672c\u91cc\u6307\u540c\u4e00\u4e8b\u4ef6\u3002\u53ef\u4ee5\u4fdd\u7559\u53cc\u65b9\u67d3\u8272\u4f53\u3001\u65b9\u5411\u548c\u4e0d\u786e\u5b9a\u533a\u95f4\uff0c\u7136\u540e\u6839\u636e\u7814\u7a76\u95ee\u9898\u51b3\u5b9a\u600e\u6837\u5339\u914d\u4e0d\u540c\u6837\u672c\u7684\u8fde\u63a5\u3002\u82e5\u4e24\u5904\u65ad\u70b9\u5728\u540c\u4e00\u4fa7\u8ddd\u79bb\u5f88\u8fd1\uff0c\u4f46\u8fde\u63a5\u5230\u5b8c\u5168\u4e0d\u540c\u7684\u8fdc\u7aef\uff0c\u53ea\u6309\u4e00\u4e2a\u5750\u6807\u805a\u7c7b\u4f1a\u8ba9\u5b83\u4eec\u6df7\u5728\u4e00\u8d77\u3002\u53cd\u8fc7\u6765\uff0c\u540c\u6e90\u533a\u7684\u540c\u4e00\u8fde\u63a5\u4e5f\u53ef\u80fd\u5728\u4e24\u6b21\u8fd0\u884c\u4e2d\u91c7\u7528\u7565\u6709\u504f\u79fb\u7684\u4f4d\u7f6e\u3002\u8f93\u51fa\u8868\u540c\u65f6\u4fdd\u7559\u4e24\u8fb9\uff0c\u624d\u6709\u673a\u4f1a\u8fa8\u8ba4\u8fd9\u4e24\u7c7b\u60c5\u51b5\u3002<\/p>\n<p>\u6700\u540e\u62bd\u68c0\u4e00\u5bf9\u6709\u88c2\u5206\u8bc1\u636e\u7684\u8fde\u63a5\u548c\u4e00\u5bf9\u4e3b\u8981\u9760\u8bfb\u5bf9\u7684\u8fde\u63a5\u3002\u5206\u522b\u67e5\u770b\u80bf\u7624\u4e0e\u6b63\u5e38\u6837\u672c\u7684\u53ef\u6bd4\u5bf9\u6027\u3001\u5c40\u90e8\u8986\u76d6\u3001\u5f02\u5e38\u8bfb\u5bf9\u65b9\u5411\u3001\u8de8\u63a5\u5e8f\u5217\u53ca\u4e24\u4fa7\u53ef\u80fd\u5b58\u5728\u7684\u91cd\u590d\u3002\u7ed9\u6bcf\u4e2a\u8fde\u63a5\u4e00\u884c\u6458\u8981\uff0c\u540c\u65f6\u4fdd\u7559\u4e24\u4e2a\u65ad\u7aef\u7684\u539f\u59cb\u8bb0\u5f55\u6807\u8bc6\u548c\u8fc7\u6ee4\u72b6\u6001\u3002\u5386\u53f2\u9879\u76ee\u5c24\u5176\u5e94\u8bb0\u5f55\u7248\u672c\u4e0e\u5b9e\u9645\u4f7f\u7528\u7684\u53c2\u8003\uff0c\u672c\u4f8b\u57fa\u4e8e\u5f52\u6863\u7684\u4e0a\u6e38\u53d1\u884c\u5305\uff0c\u80fd\u591f\u590d\u73b0\u968f\u5305\u793a\u4f8b\u8fd9\u4ef6\u4e8b\u672c\u8eab\u4e0d\u80fd\u8bc1\u660e\u4efb\u4e00\u65b0\u6837\u672c\u7684\u65ad\u70b9\u5c31\u5df2\u88ab\u9a8c\u8bc1\u3002<a style=\"color:#226985;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/Illumina\/manta\">Manta \u4e0a\u6e38\u4ed3\u5e93\u4e0e\u7248\u672c\u72b6\u6001<\/a><\/p>\n<p class=\"guide-end\" style=\"margin-top:1.6em;padding-top:1.0em;border-top:1px solid #d7e0e9\">\u8981\u5728\u6574\u6279\u80bf\u7624\u4e0e\u6b63\u5e38\u914d\u5bf9\u6570\u636e\u4e0a\u590d\u8dd1\u8fd9\u79cd\u65ad\u7aef\u6838\u67e5\uff0c\u53ef\u4f7f\u7528<a data-ysx-event=\"contact_click\" style=\"color:#226985;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/www.biocloudservice.com\/wordpress\/compute-services\/shared-research-workspace\/\">\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668<\/a>\u4fdd\u5b58\u6210\u5bf9\u7684\u539f\u59cb\u8bc1\u636e\u4e0e\u6c47\u603b\u7ed3\u679c\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u7528\u771f\u5b9e\u6f14\u793a\u8f93\u51fa\u8fd8\u539f\u4e09\u5bf9\u4e92\u6307\u65ad\u7aef\uff0c\u6838\u5bf9\u5fae\u540c\u6e90\u3001\u5bbd\u533a\u95f4\u3001\u66ff\u4ee3\u652f\u6301\u4e0e\u672a\u62a5\u544a\u7684\u88c2\u5206\u8bfb\u6570\u3002<\/p>\n","protected":false},"author":1,"featured_media":70115,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[989,988,578,990],"class_list":["post-70116","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-bnd","tag-manta","tag-578","tag-990"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/manta-breakend-pairs-mateid-split-read-evidence-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70116","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=70116"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70116\/revisions"}],"predecessor-version":[{"id":70118,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70116\/revisions\/70118"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/70115"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=70116"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=70116"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=70116"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}