{"id":70435,"date":"2026-11-14T23:51:11","date_gmt":"2026-11-14T15:51:11","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=70435"},"modified":"2026-11-14T23:51:11","modified_gmt":"2026-11-14T15:51:11","slug":"parabricks-fq2bam-cigar-three-lengths-coordinate-audit","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/parabricks-fq2bam-cigar-three-lengths-coordinate-audit\/","title":{"rendered":"Parabricks FQ2BAM \u9a8c\u6536\uff5c\u7528\u4e00\u6761\u5b98\u65b9\u6bd4\u5bf9\u8bb0\u5f55\u7b97\u6e05\u8bfb\u957f\u3001\u8986\u76d6\u8303\u56f4\u548c\u7247\u6bb5\u957f\u5ea6"},"content":{"rendered":"
\n

\u62ff\u5230\u4e00\u4efd\u53cc\u7aef\u6bd4\u5bf9\u7ed3\u679c\uff0c\u540c\u4e8b\u95ee\u8fd9\u6761\u8bb0\u5f55\u6709\u591a\u957f\uff0c\u4e09\u4e2a\u56de\u7b54\u53ef\u80fd\u90fd\u5bf9\u3002\u6d4b\u5e8f\u4eea\u4ea4\u51fa\u4e86\u591a\u5c11\u4e2a\u78b1\u57fa\uff0c\u8fd9\u4e00\u7aef\u6a2a\u8de8\u53c2\u8003\u4e0a\u7684\u591a\u5c11\u4e2a\u4f4d\u7f6e\uff0c\u8fd9\u4e00\u5bf9\u8bfb\u6bb5\u6765\u81ea\u591a\u957f\u7684\u7247\u6bb5\uff0c\u5206\u522b\u8981\u8bfb\u4e0d\u540c\u7684\u5b57\u6bb5\u3002NVIDIA \u7684 FQ2BAM \u6559\u7a0b\u6070\u597d\u5217\u51fa\u4e00\u6761\u540c\u65f6\u5e26\u6709\u63d2\u5165\u3001\u914d\u5bf9\u5750\u6807\u548c\u53e6\u4e00\u7aef\u6bd4\u5bf9\u6458\u8981\u7684\u8bb0\u5f55\u3002\u6211\u4eec\u7528 SAMtools \u628a\u5b83\u505a\u6210\u53ef\u67e5\u8be2\u7684\u6587\u4ef6\uff0c\u9010\u9879\u9a8c\u8bc1\u4e09\u4e2a\u957f\u5ea6\uff0c\u6700\u540e\u518d\u8003\u5bdf\u4e00\u4e2a\u5f88\u5bb9\u6613\u8bef\u89e3\u7684\u73b0\u8c61\u3002\u660e\u660e\u6807\u8bb0\u6210\u4e86\u6b63\u5e38\u914d\u5bf9\uff0c\u5728\u53e6\u4e00\u7aef\u7684\u5750\u6807\u4e0a\u5374\u53ef\u4ee5\u4e00\u6761\u8bb0\u5f55\u4e5f\u53d6\u4e0d\u5230\u3002NVIDIA FQ2BAM \u6559\u7a0b<\/a><\/p>\n

\u6536\u5230\u7ed3\u679c\u5148\u770b\u4e00\u6761\u80fd\u7b97\u6e05\u695a\u7684\u8bb0\u5f55<\/h2>\n

FQ2BAM \u63a5\u6536\u53c2\u8003\u5e8f\u5217\u548c\u6210\u5bf9\u7684\u539f\u59cb\u8bfb\u6bb5\uff0c\u751f\u6210\u6309\u53c2\u8003\u5750\u6807\u6392\u5e8f\u7684\u6bd4\u5bf9\u6587\u4ef6\u3002\u6838\u5bf9\u65f6\u5148\u8ddf\u5bf9\u4e00\u4e2a\u7247\u6bb5\u4ece\u54ea\u91cc\u6765\uff0c\u628a\u539f\u59cb\u8bfb\u6bb5\u540d\u3001\u6837\u672c\u540d\u3001\u6d4b\u5e8f\u6279\u6b21\u3001\u4f7f\u7528\u7684\u53c2\u8003\u7248\u672c\u7559\u5728\u8bb0\u5f55\u4e2d\u3002\u4e0d\u540c\u6765\u6e90\u7684\u6837\u672c\u90fd\u53ef\u80fd\u8986\u76d6\u540c\u4e00\u4e2a\u4f4d\u7f6e\uff0c\u56e0\u6b64\u53d6\u5230\u4e00\u6761\u770b\u4f3c\u5408\u7406\u7684\u8bb0\u5f55\uff0c\u5e76\u4e0d\u8db3\u4ee5\u8bf4\u660e\u4ea4\u63a5\u7684\u662f\u9884\u671f\u6837\u672c\u3002\u751f\u4ea7\u6570\u636e\u53ef\u4ee5\u6309\u8bfb\u7ec4\u8ffd\u5230\u6837\u672c\u548c\u6587\u5e93\uff0c\u6d4f\u89c8\u5668\u663e\u793a\u7684\u67d3\u8272\u4f53\u524d\u7f00\u4e5f\u8981\u4e0e\u6587\u4ef6\u5934\u4e00\u81f4\u3002\u63a5\u4e0b\u6765\u6240\u6709\u5750\u6807\u90fd\u91c7\u7528 SAM \u7684\u4e00\u57fa\u3001\u4e24\u7aef\u5305\u542b\u7ea6\u5b9a\u3002<\/p>\n

\u6211\u4eec\u53d6\u7684\u662f\u5b98\u65b9\u6559\u7a0b\u5c55\u793a\u7684\u9996\u6761\u6570\u636e\uff0c\u5728\u672c\u5730\u8865\u4e00\u4e2a\u53ea\u542b\u6240\u9700\u53c2\u8003\u6761\u76ee\u7684\u6700\u5c0f\u6587\u4ef6\u5934\uff0c\u7528\u5b9e\u9645\u5b89\u88c5\u7684 SAMtools 1.19.2 \u8f6c\u6210 BAM \u5e76\u5efa\u7d22\u5f15\u3002\u4fdd\u7559\u5b98\u65b9\u8bb0\u5f55\u539f\u6709\u7684\u65b9\u5411\u3001\u78b1\u57fa\u3001\u8d28\u91cf\u3001\u4f4d\u7f6e\u548c\u6807\u7b7e\uff0c\u4e0d\u4e3a\u914d\u5076\u7f16\u9020\u7b2c\u4e8c\u6761\u8bb0\u5f55\u3002\u8bfb\u8005\u53ef\u4ee5\u5bf9\u81ea\u5df1\u7684\u9a8c\u6536\u6837\u672c\u505a\u540c\u6837\u7684\u4e8b\uff0c\u4e8b\u5148\u5199\u4e0b\u9884\u671f\u7684\u56db\u4e2a\u8fb9\u754c\u518d\u67e5\u8be2\u3002\u6559\u7a0b\u91cc\u7684 GPU \u7a0b\u5e8f\u548c\u9636\u6bb5\u65f6\u95f4\u51fa\u81ea NVIDIA \u7684\u793a\u4f8b\uff0c\u672c\u6b21\u672c\u5730\u8fd0\u884c\u7684\u662f\u4e0b\u6e38\u683c\u5f0f\u68c0\u67e5\u4e0e\u533a\u95f4\u67e5\u8be2\u3002SAM \u683c\u5f0f\u89c4\u8303<\/a><\/p>\n

\u8bfb\u957f\u4e3a 115\uff0c\u53c2\u8003\u8de8\u5ea6\u4e3a 110<\/h2>\n

\u8be5\u8bb0\u5f55\u4ece\u4e00\u53f7\u67d3\u8272\u4f53\u7684 10027 \u5f00\u59cb\u3002\u628a\u6bd4\u5bf9\u4e32\u6309\u64cd\u4f5c\u62c6\u5f00\uff0c\u4f9d\u6b21\u662f\u4e8c\u5341\u56db\u4e2a\u53c2\u4e0e\u5339\u914d\u7684\u78b1\u57fa\u3001\u4e94\u4e2a\u76f8\u5bf9\u53c2\u8003\u7684\u63d2\u5165\u3001\u516b\u5341\u516d\u4e2a\u53c2\u4e0e\u5339\u914d\u7684\u78b1\u57fa\u3002\u8981\u56de\u7b54\u6d4b\u4e86\u591a\u957f\uff0c\u5c31\u628a\u6d88\u8017\u67e5\u8be2\u5e8f\u5217\u7684\u4e09\u6bb5\u76f8\u52a0\uff0c\u5f97\u5230\u4e00\u767e\u4e00\u5341\u4e94\u3002\u8981\u56de\u7b54\u5728\u53c2\u8003\u4e0a\u8d70\u4e86\u591a\u8fdc\uff0c\u53ea\u52a0\u4e24\u6bb5\u5339\u914d\u64cd\u4f5c\uff0c\u5f97\u5230\u4e00\u767e\u4e00\u5341\u3002\u8d77\u70b9\u672c\u8eab\u5360\u7b2c\u4e00\u4e2a\u4f4d\u7f6e\uff0c\u6240\u4ee5\u672b\u5750\u6807\u8fd8\u8981\u51cf\u4e00\u3002\u76f4\u63a5\u6570\u8fd9\u6761\u8bb0\u5f55\u7684\u5e8f\u5217\u5b57\u7b26\u4e32\u548c\u8d28\u91cf\u5b57\u7b26\u4e32\uff0c\u4e24\u8005\u957f\u5ea6\u4e5f\u90fd\u662f\u4e00\u767e\u4e00\u5341\u4e94\u3002<\/p>\n

\u5339\u914d\u64cd\u4f5c\u5141\u8bb8\u78b1\u57fa\u76f8\u540c\u6216\u4e0d\u540c\u3002\u82e5\u60f3\u77e5\u9053\u53c2\u8003\u4e0e\u8bfb\u6bb5\u5b9e\u9645\u5dee\u591a\u5c11\uff0c\u9700\u8981\u518d\u8bfb\u7f16\u8f91\u8ddd\u79bb\u4e0e\u9519\u914d\u63cf\u8ff0\u3002\u4f8b\u5b50\u91cc\u7684\u9519\u914d\u63cf\u8ff0\u663e\u793a\u516d\u6b21\u53c2\u8003\u78b1\u57fa\u66ff\u6362\uff0c\u5176\u4e2d\u4e24\u5904\u4e4b\u95f4\u76f8\u9694\u96f6\u4e2a\u5339\u914d\u78b1\u57fa\u3002\u8fd9\u4e2a\u96f6\u662f\u4e24\u4e2a\u8fde\u7eed\u9519\u914d\u4e4b\u95f4\u7684\u95f4\u9694\u3002\u518d\u4ece\u6bd4\u5bf9\u4e32\u52a0\u5165\u63d2\u5165\u7684\u4e94\u4e2a\u78b1\u57fa\uff0c\u6b63\u597d\u4e0e\u7f16\u8f91\u8ddd\u79bb\u5341\u4e00\u76f8\u5408\u3002\u628a\u7f16\u8f91\u8ddd\u79bb\u76f4\u63a5\u8bb0\u6210\u5341\u4e00\u5904\u5355\u78b1\u57fa\u66ff\u6362\uff0c\u6216\u8005\u628a\u4e24\u6bb5\u5339\u914d\u64cd\u4f5c\u5168\u90e8\u7b97\u4f5c\u7cbe\u786e\u76f8\u540c\uff0c\u90fd\u4f1a\u8ba9\u9519\u8bef\u7c7b\u578b\u7edf\u8ba1\u5931\u771f\u3002\u9047\u5230\u53c2\u8003\u7f3a\u5931\u65f6\u8fd8\u8981\u8bc6\u522b\u9519\u914d\u63cf\u8ff0\u91cc\u4ee5\u5c16\u53f7\u5f00\u5934\u7684\u5220\u9664\u4e32\uff0c\u4e0d\u80fd\u628a\u5176\u4e2d\u5b57\u6bcd\u9010\u4e00\u5f53\u4f5c\u66ff\u6362\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n
\u60f3\u6838\u5bf9\u4ec0\u4e48<\/th>\n\u8bfb\u53d6\u4ec0\u4e48<\/th>\n\u672c\u4f8b\u5b9e\u6d4b<\/th>\n<\/tr>\n<\/thead>\n
\u67e5\u8be2\u5e8f\u5217\u957f\u5ea6<\/td>\n24M5I86M<\/code> \u4e2d\u7684 M\u3001I<\/td>\n115 \u4e2a\u78b1\u57fa<\/td>\n<\/tr>\n
\u8fd9\u4e00\u7aef\u7684\u53c2\u8003\u8de8\u5ea6<\/td>\n\u4e24\u6bb5 M\uff0c\u52a0\u4e0a\u4e00\u57fa\u8d77\u70b9<\/td>\n10027 \u81f3 10136\uff0c\u5171 110 \u4e2a\u4f4d\u7f6e<\/td>\n<\/tr>\n
\u914d\u5076\u7684\u53c2\u8003\u8de8\u5ea6<\/td>\n34S11M1I69M<\/code> \u4e2d\u7684\u4e24\u6bb5 M<\/td>\n10178 \u81f3 10257\uff0c\u5171 80 \u4e2a\u4f4d\u7f6e<\/td>\n<\/tr>\n
\u6574\u4e2a\u7247\u6bb5\u7684\u5916\u4fa7\u8de8\u5ea6<\/td>\n\u4e24\u7aef\u6700\u5916\u4fa7\u7684\u53c2\u8003\u5750\u6807<\/td>\n231 \u4e2a\u4f4d\u7f6e<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u540c\u4e00\u6761\u8bb0\u5f55\u6709\u4e09\u4e2a\u957f\u5ea6\u3002NVIDIA
NVIDIA \u5b98\u65b9\u6bd4\u5bf9\u5b9e\u4f8b\uff0c\u6309\u4e00\u57fa\u5750\u6807\u6838\u5bf9\u8bfb\u957f\u3001\u53c2\u8003\u8de8\u5ea6\u4e0e\u7247\u6bb5\u5916\u4fa7\u7aef\u70b9\u3002<\/figcaption><\/figure>\n

\u914d\u5076\u7684\u8f6f\u526a\u5207\u53bb\u4e86\u54ea\u91cc<\/h2>\n

\u5341\u8fdb\u5236\u6807\u5fd7\u4e5d\u5341\u4e5d\u7ecf\u5de5\u5177\u89e3\u7801\uff0c\u8868\u660e\u8fd9\u662f\u6210\u5bf9\u6d4b\u5e8f\u4e2d\u7b2c\u4e00\u7aef\uff0c\u8f6f\u4ef6\u5c06\u5176\u6807\u4e3a\u6b63\u5e38\u914d\u5bf9\uff0c\u53e6\u4e00\u7aef\u843d\u5728\u53cd\u5411\u94fe\u3002\u8fd9\u4e2a\u6807\u5fd7\u53ea\u8bf4\u8bb0\u5f55\u81ea\u5e26\u7684\u72b6\u6001\u3002\u8981\u8ffd\u8e2a\u53e6\u4e00\u7aef\uff0c\u5148\u8bfb\u914d\u5076\u53c2\u8003\u540d\u79f0\u548c\u8d77\u70b9\uff0c\u518d\u770b\u914d\u5076\u6bd4\u5bf9\u6458\u8981\u3002\u4f8b\u5b50\u4e2d\u7684\u53e6\u4e00\u7aef\u6709\u4e09\u5341\u56db\u4e2a\u8f6f\u526a\u5207\u78b1\u57fa\uff0c\u5b83\u4eec\u4fdd\u7559\u5728\u90a3\u4e00\u7aef\u7684\u67e5\u8be2\u5e8f\u5217\u4e2d\uff0c\u5728\u53c2\u8003\u4e0a\u4e0d\u5411\u524d\u63a8\u8fdb\u3002\u8fd8\u6709\u4e00\u4e2a\u63d2\u5165\u4e5f\u53ea\u5360\u67e5\u8be2\u3002\u7531\u6b64\u53ef\u4ee5\u72ec\u7acb\u7b97\u51fa\u914d\u5076\u672c\u8eab\u6709\u4e00\u767e\u4e00\u5341\u4e94\u4e2a\u78b1\u57fa\uff0c\u6a2a\u8de8\u7684\u53c2\u8003\u5374\u53ea\u6709\u516b\u5341\u4e2a\u4f4d\u7f6e\u3002<\/p>\n

\u672c\u4f8b\u4e24\u7aef\u4f4d\u4e8e\u540c\u4e00\u53c2\u8003\uff0c\u5916\u4fa7\u7aef\u70b9\u76f8\u8ddd\u4e8c\u767e\u4e09\u5341\u4e00\u4e2a\u4f4d\u7f6e\uff0c\u6070\u597d\u7b49\u4e8e\u7b2c\u4e00\u7aef\u8bb0\u5f55\u91cc\u7ed9\u51fa\u7684\u6b63\u7247\u6bb5\u957f\u5ea6\u3002\u5982\u679c\u628a\u4e24\u6761\u8bfb\u957f\u76f4\u63a5\u76f8\u52a0\uff0c\u5f97\u5230\u4e8c\u767e\u4e09\u5341\uff0c\u6570\u503c\u53ea\u5dee\u4e00\uff0c\u7406\u7531\u5374\u5b8c\u5168\u4e0d\u540c\u3002\u4e24\u4e2a\u8bfb\u6bb5\u4e4b\u95f4\u7559\u6709\u672a\u88ab\u8fd9\u4e24\u7aef\u8986\u76d6\u7684\u533a\u95f4\uff0c\u914d\u5076\u524d\u90e8\u53c8\u53d1\u751f\u8f6f\u526a\u5207\u3002\u672c\u4f8b\u80fd\u4ece\u914d\u5076\u6807\u7b7e\u4ea4\u53c9\u9a8c\u8bc1\u5916\u4fa7\u7aef\u70b9\uff0c\u662f\u56e0\u4e3a\u6807\u7b7e\u9f50\u5168\u3002\u5b9e\u9645\u6587\u4ef6\u53ef\u80fd\u6ca1\u6709\u8fd9\u4e2a\u53ef\u9009\u6807\u7b7e\uff0c\u6b64\u65f6\u8981\u53d6\u56de\u914d\u5076\u8bb0\u5f55\uff0c\u4ece\u5b83\u81ea\u5df1\u7684\u6bd4\u5bf9\u4e32\u91cd\u7b97\u3002\u9047\u5230\u8de8\u67d3\u8272\u4f53\u548c\u8865\u5145\u6bd4\u5bf9\u65f6\u4e5f\u8981\u91cd\u65b0\u786e\u8ba4\u7247\u6bb5\u957f\u5ea6\u5b57\u6bb5\u662f\u5426\u6709\u53ef\u89e3\u91ca\u7684\u542b\u4e49\u3002<\/p>\n

\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u7528\u4e24\u4e2a\u521a\u597d\u843d\u7a7a\u7684\u5750\u6807\u68c0\u67e5\u7b97\u6cd5<\/h2>\n
samtools flags 99\r\nsamtools view -b -o one.bam one.sam\r\nsamtools index one.bam\r\nsamtools view -c one.bam chr1:10027-10027\r\nsamtools view -c one.bam chr1:10136-10136\r\nsamtools view -c one.bam chr1:10137-10137\r\nsamtools view -c one.bam chr1:10178-10178\r\n<\/code><\/pre>\n
\u5206\u522b\u67e5\u8be2\u8d77\u70b9\u524d\u4e00\u4f4d\u3001\u8d77\u70b9\u3001\u672b\u4f4d\u3001\u672b\u4f4d\u540e\u4e00\u4f4d\uff0c\u8fd4\u56de\u6570\u4f9d\u6b21\u662f\u96f6\u3001\u4e00\u3001\u4e00\u3001\u96f6\u3002\u914d\u5076\u8d77\u70b9\u5904\u4e5f\u8fd4\u56de\u96f6\u3002\u539f\u56e0\u53ef\u4ee5\u76f4\u63a5\u4ece\u5b9e\u9a8c\u8bbe\u8ba1\u627e\u5230\uff0c\u91cd\u5efa\u7684\u6587\u4ef6\u53ea\u5305\u542b\u7b2c\u4e00\u7aef\uff0c\u7d22\u5f15\u6309\u6587\u4ef6\u91cc\u771f\u5b9e\u5b58\u5728\u7684\u6bd4\u5bf9\u8bb0\u5f55\u5efa\u7acb\u3002\u4e00\u4e2a\u6807\u7b7e\u91cc\u63d0\u5230\u53e6\u4e00\u4e2a\u4f4d\u7f6e\uff0c\u4e0d\u4f1a\u5728\u8be5\u5904\u51ed\u7a7a\u751f\u6210\u7b2c\u4e8c\u6761\u8bb0\u5f55\u3002\u4ee5\u540e\u6709\u4eba\u7528\u4e00\u4e2a\u7a84\u7a97\u53e3\u622a\u51fa\u53cc\u7aef\u6570\u636e\uff0c\u518d\u62ff\u5b83\u8bc4\u4f30\u6210\u5bf9\u6062\u590d\u7387\uff0c\u5148\u95ee\u622a\u53d6\u65b9\u6cd5\u662f\u5426\u540c\u65f6\u627e\u56de\u4e86\u843d\u5728\u7a97\u53e3\u4ee5\u5916\u7684\u914d\u5076\u3002\u6574\u5e93\u4e0e\u5c0f\u7a97\u53e3\u7684\u5206\u6bcd\u5f80\u5f80\u5c31\u5728\u8fd9\u91cc\u53d1\u751f\u53d8\u5316\u3002SAMtools \u533a\u95f4\u8bfb\u53d6\u8bf4\u660e<\/a><\/p>\n
\u533a\u57df\u67e5\u8be2\u8fd4\u56de\u7684\u662f\u6bd4\u5bf9\u8bb0\u5f55\u7684\u91cd\u53e0\u5173\u7cfb\u3002\u82e5\u4e0b\u4e00\u6b65\u8981\u4ea4\u4ed8\u8986\u76d6\u6df1\u5ea6\uff0c\u9700\u8981\u53e6\u5916\u5b9a\u4e49\u78b1\u57fa\u8d28\u91cf\u3001\u6bd4\u5bf9\u8d28\u91cf\u3001\u5220\u9664\u4f4d\u7f6e\u548c\u91cd\u53e0\u53cc\u7aef\u5404\u5982\u4f55\u8ba1\u6570\u3002\u5c24\u5176\u5728\u6709\u8f83\u5927\u7f3a\u5931\u6216\u8de8\u5185\u542b\u5b50\u6bd4\u5bf9\u65f6\uff0c\u4e00\u6761\u8bb0\u5f55\u4ece\u533a\u95f4\u5de6\u8fb9\u5ef6\u4f38\u5230\u53f3\u8fb9\uff0c\u533a\u95f4\u4e2d\u592e\u5374\u53ef\u80fd\u6ca1\u6709\u4efb\u4f55\u88ab\u6d4b\u5230\u7684\u78b1\u57fa\u3002\u6240\u4ee5\u8fd9\u7ec4\u8fb9\u754c\u6d4b\u8bd5\u9002\u5408\u68c0\u67e5\u5750\u6807\u6362\u7b97\uff0c\u6df1\u5ea6\u9a8c\u6536\u8fd8\u8981\u53d6\u540c\u4e00\u5750\u6807\u67e5\u770b\u5806\u53e0\u7ed3\u679c\u3002\u628a\u8fd9\u4e24\u4e2a\u95ee\u9898\u5206\u5f00\u8bb0\u5f55\uff0c\u540e\u7eed\u624d\u80fd\u89e3\u91ca\u6d4f\u89c8\u5668\u91cc\u4e00\u4e2a\u957f\u6761\u548c\u6df1\u5ea6\u8868\u91cc\u4e00\u4e2a\u96f6\u4e3a\u4ec0\u4e48\u53ef\u4ee5\u540c\u65f6\u6210\u7acb\u3002<\/p>\n
\u5b98\u65b9\u65e5\u5fd7\u53e6\u6709\u4e09\u4e2a\u9636\u6bb5\u5206\u522b\u7528\u65f6\u4e94\u5341\u3001\u4e94\u548c\u5341\u4e03\u79d2\uff0c\u76f8\u52a0\u4e3a\u4e03\u5341\u4e8c\u79d2\uff0c\u4ece\u9996\u6bb5\u5f00\u59cb\u5230\u672b\u6bb5\u7ed3\u675f\u7684\u65f6\u949f\u8de8\u5ea6\u5374\u662f\u4e03\u5341\u56db\u79d2\u3002\u505a\u6d41\u7a0b\u9a8c\u6536\u65f6\uff0c\u5c06\u9636\u6bb5\u7edf\u8ba1\u548c\u7ecf\u8fc7\u65f6\u95f4\u5206\u522b\u8bb0\u5728\u4e24\u680f\uff0c\u5e76\u6ce8\u660e\u6570\u636e\u89c4\u6a21\u3001\u662f\u5426\u5305\u62ec\u6392\u5e8f\u4e0e\u91cd\u590d\u6807\u8bb0\u3002\u8fd9\u6837\u540c\u4e8b\u65e2\u80fd\u590d\u6838\u7ed3\u679c\u6587\u4ef6\uff0c\u53c8\u80fd\u770b\u61c2\u65f6\u95f4\u662f\u4ece\u54ea\u4e00\u6b65\u5f00\u59cb\u8ba1\u7684\u3002<\/p>\n
\u628a\u8fd9\u5957\u5c0f\u7a97\u53e3\u9a8c\u6536\u7528\u5230\u6574\u6279\u5168\u57fa\u56e0\u7ec4\u6570\u636e\u65f6\uff0c\u5982\u679c\u8fd8\u8981\u957f\u671f\u4fdd\u7559\u539f\u59cb\u8bfb\u6bb5\u548c\u6bd4\u5bf9\u7ed3\u679c\u4f9b\u591a\u4eba\u6838\u67e5\uff0c\u53ef\u4ee5\u4e86\u89e3\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668<\/a>\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u4ece\u5b98\u65b9 FQ2BAM \u8f93\u51fa\u91cd\u5efa\u4e00\u6761\u53ef\u7d22\u5f15\u7684 BAM \u8bb0\u5f55\uff0c\u5b9e\u6d4b\u533a\u95f4\u8fb9\u754c\u3001\u914d\u5076\u4f4d\u7f6e\u548c\u4e09\u4e2a\u5bb9\u6613\u6df7\u6dc6\u7684\u957f\u5ea6\u3002<\/p>\n","protected":false},"author":1,"featured_media":70434,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[1022,1021,964,436],"class_list":["post-70435","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-fq2bam","tag-parabricks","tag-samtools","tag-436"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/parabricks-fq2bam-cigar-three-lengths-coordinate-audit-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70435","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=70435"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70435\/revisions"}],"predecessor-version":[{"id":70446,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70435\/revisions\/70446"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/70434"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=70435"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=70435"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=70435"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}