{"id":70437,"date":"2026-11-14T23:51:13","date_gmt":"2026-11-14T15:51:13","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=70437"},"modified":"2026-11-14T23:51:13","modified_gmt":"2026-11-14T15:51:13","slug":"svaba-local-assembly-24bp-tumor-insertion","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/svaba-local-assembly-24bp-tumor-insertion\/","title":{"rendered":"SvABA \u5b9e\u8dd1\uff5c\u4e00\u4e2a 24 bp \u7684\u63d2\u5165\uff0c\u600e\u6837\u4ece\u5c40\u90e8\u7ec4\u88c5\u4e00\u8def\u8ffd\u5230\u80bf\u7624\u548c\u6b63\u5e38\u6837\u672c\uff1f"},"content":{"rendered":"<div class=\"ysx-method-guide\" style=\"max-width:820px;min-width:0;margin:0 auto;color:#26374a;line-height:1.86;overflow-wrap:anywhere\">\n<p>\u4e00\u4efd\u4f53\u7ec6\u80de\u53d8\u5f02\u8868\u91cc\u51fa\u73b0\u4e86\u4e8c\u5341\u56db\u4e2a\u989d\u5916\u78b1\u57fa\u3002\u590d\u6838\u65f6\u6709\u4e09\u4e2a\u6734\u7d20\u7684\u95ee\u9898\u503c\u5f97\u9010\u4e2a\u56de\u7b54\u3002\u591a\u51fa\u6765\u7684\u5e8f\u5217\u662f\u4ec0\u4e48\uff0c\u5b83\u63a5\u5728\u53c2\u8003\u7684\u54ea\u4e2a\u78b1\u57fa\u4e4b\u540e\uff0c\u6b63\u5e38\u6837\u672c\u5728\u540c\u4e00\u4f4d\u7f6e\u5230\u5e95\u6709\u6ca1\u6709\u8db3\u591f\u7684\u89c2\u6d4b\uff1fSvABA \u9664\u4e86\u6700\u7ec8\u7684\u53d8\u5f02\u8868\uff0c\u8fd8\u80fd\u7559\u4e0b\u5c40\u90e8\u7ec4\u88c5\u5e8f\u5217\u91cd\u65b0\u6bd4\u56de\u53c2\u8003\u7684\u7ed3\u679c\u3002\u628a\u8fd9\u4e24\u4efd\u6587\u4ef6\u4e0e\u4e24\u4efd\u6837\u672c\u7684\u652f\u6301\u6570\u5b57\u5bf9\u9f50\uff0c\u5c31\u53ef\u4ee5\u4ece\u4e00\u6761\u5019\u9009\u8bb0\u5f55\u56de\u5230\u5b9e\u9a8c\u672c\u8eab\u3002\u4e0b\u9762\u7528\u4e00\u4e2a\u4e8b\u5148\u57cb\u597d\u63d2\u5165\u7684\u4f8b\u5b50\u8d70\u5b8c\u5168\u7a0b\u3002<a style=\"color:#984322;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/walaj\/svaba\">SvABA \u9879\u76ee\u4e0e\u8f93\u51fa\u8bf4\u660e<\/a><\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u7ed9\u5b9e\u9a8c\u653e\u4e00\u4e2a\u77e5\u9053\u7b54\u6848\u7684\u4f4d\u70b9<\/h2>\n<p>\u6211\u4eec\u56fa\u5b9a\u968f\u673a\u79cd\u5b50\uff0c\u751f\u6210\u4e00\u6761\u4e24\u4e07\u78b1\u57fa\u7684\u53c2\u8003\u5e8f\u5217\uff0c\u5728\u7b2c\u4e00\u4e07\u4f4d\u4e4b\u540e\u52a0\u5165\u4e00\u6bb5\u4e8c\u5341\u56db\u78b1\u57fa\u7684\u5e8f\u5217\uff0c\u5f62\u6210\u53e6\u4e00\u6761\u5355\u500d\u578b\u3002\u6b63\u5e38\u6837\u672c\u7684\u6240\u6709\u7247\u6bb5\u90fd\u4ece\u539f\u53c2\u8003\u62bd\u6837\u3002\u80bf\u7624\u6837\u672c\u7684\u4e00\u534a\u7247\u6bb5\u4ece\u539f\u53c2\u8003\u62bd\u6837\uff0c\u53e6\u4e00\u534a\u4ece\u5e26\u63d2\u5165\u7684\u6a21\u677f\u62bd\u6837\uff0c\u4e24\u79cd\u6765\u6e90\u4ea4\u66ff\u53d6\u6837\u3002\u4e24\u4efd\u6587\u5e93\u5404\u56db\u5343\u4e8c\u767e\u5bf9\u8bfb\u6bb5\uff0c\u6bcf\u7aef\u4e00\u767e\u4e00\u5341\u4e2a\u78b1\u57fa\uff0c\u7247\u6bb5\u957f\u5ea6\u56f4\u7ed5\u4e09\u767e\u4e00\u5341\u4e94\u4e2a\u78b1\u57fa\u6ce2\u52a8\u3002\u4e24\u4e2a\u6837\u672c\u7684\u62bd\u6837\u76f8\u4e92\u72ec\u7acb\uff0c\u54ea\u6761\u8bfb\u6bb5\u6765\u81ea\u54ea\u4e2a\u7247\u6bb5\uff0c\u90fd\u80fd\u901a\u8fc7\u540d\u5b57\u8ffd\u8e2a\u3002\u8fd9\u6837\u8bbe\u8ba1\u7684\u597d\u5904\u662f\uff0c\u5373\u4f7f\u6700\u540e\u4ec0\u4e48\u90fd\u6ca1\u6709\u62a5\u51fa\uff0c\u4e5f\u77e5\u9053\u5e94\u8be5\u56de\u5230\u54ea\u4e2a\u4f4d\u7f6e\u627e\u8bc1\u636e\u3002<\/p>\n<p>\u4e24\u4efd\u6570\u636e\u5148\u5404\u81ea\u7528 BWA \u6bd4\u56de\u540c\u4e00\u6761\u672a\u63d2\u5165\u7684\u53c2\u8003\uff0c\u518d\u6392\u5e8f\u5efa\u7d22\u5f15\u3002\u968f\u540e\u7528 SvABA 1.2.0 \u5206\u522b\u4ee5\u5c40\u90e8\u7ec4\u88c5\u548c\u4ec5\u68c0\u6d4b\u5f02\u5e38\u914d\u5bf9\u4e24\u79cd\u6a21\u5f0f\u8fd0\u884c\u3002\u4e24\u8f6e\u4f7f\u7528\u540c\u4e00\u5bf9\u80bf\u7624\u3001\u6b63\u5e38\u8f93\u5165\uff0c\u540c\u4e00\u6bb5\u76ee\u6807\u533a\u95f4\u548c\u540c\u4e00\u53c2\u8003\u3002\u5982\u679c\u6362\u6a21\u5f0f\u65f6\u540c\u65f6\u6362\u4e86\u6b63\u5e38\u5bf9\u7167\uff0c\u5019\u9009\u7684\u6d88\u5931\u5c31\u6709\u591a\u79cd\u89e3\u91ca\u3002\u8fd9\u91cc\u628a\u53d8\u91cf\u63a7\u5236\u4e3a\u5206\u6790\u6a21\u5f0f\uff0c\u4e5f\u7279\u610f\u4fdd\u7559\u4e24\u8f6e\u7684\u672a\u8fc7\u6ee4\u7ed3\u679c\uff0c\u4ee5\u4fbf\u533a\u5206\u6ca1\u6709\u751f\u6210\u5019\u9009\u548c\u751f\u6210\u4e4b\u540e\u88ab\u8fc7\u6ee4\u8fd9\u4e24\u4ef6\u4e8b\u3002<a style=\"color:#984322;text-decoration:underline;text-underline-offset:2px\" href=\"https:\/\/github.com\/walaj\/svaba\/releases\/tag\/v1.2.0\">\u672c\u6b21\u4f7f\u7528\u7684 SvABA \u7248\u672c<\/a><\/p>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u7b2c\u4e00\u6761\u8bc1\u636e\u662f\u63d2\u5165\u4e24\u4fa7\u7684\u951a\u70b9<\/h2>\n<p>\u7ec4\u88c5\u6a21\u5f0f\u5728\u7ecf\u8fc7\u8fc7\u6ee4\u7684\u4f53\u7ec6\u80de\u5c0f\u63d2\u5165\u7f3a\u5931\u6587\u4ef6\u91cc\u6070\u597d\u62a5\u51fa\u4e00\u6761\uff0c\u5728\u5bf9\u5e94\u7684\u672a\u8fc7\u6ee4\u6587\u4ef6\u91cc\u4e5f\u6709\u4e00\u6761\u3002\u6309\u67d3\u8272\u4f53\u3001\u4f4d\u7f6e\u3001\u53c2\u8003\u548c\u66ff\u4ee3\u5e8f\u5217\u9010\u9879\u6838\u5bf9\uff0c\u4e24\u6761\u5b8c\u5168\u76f8\u540c\u3002\u5176\u4f59\u516d\u4efd\u53d8\u5f02\u8868\u4e2d\u6ca1\u6709\u6570\u636e\u884c\u3002\u82e5\u628a\u6240\u6709\u8f93\u51fa\u6587\u4ef6\u5408\u5e76\u540e\u6309\u8bb0\u5f55\u6570\u62a5\u4e24\u4e2a\u53d8\u5f02\uff0c\u540c\u4e00\u4e8b\u4ef6\u5c31\u88ab\u91cd\u590d\u7edf\u8ba1\u3002SvABA \u5c06\u5c0f\u63d2\u5165\u7f3a\u5931\u4e0e\u7ed3\u6784\u53d8\u5f02\u5206\u5f00\u8f93\u51fa\uff0c\u53c8\u533a\u5206\u4f53\u7ec6\u80de\u548c\u751f\u6b96\u7cfb\uff0c\u5e76\u4fdd\u7559\u8fc7\u6ee4\u524d\u7684\u5019\u9009\u3002\u76d8\u70b9\u65f6\u8bb0\u4e0b\u6bcf\u4e2a\u8f93\u5165\u6587\u4ef6\u7684\u53e3\u5f84\uff0c\u6bd4\u5728\u5408\u5e76\u540e\u731c\u4e00\u6761\u8bb0\u5f55\u5c5e\u4e8e\u54ea\u4e00\u6279\u53ef\u9760\u5f97\u591a\u3002<\/p>\n<p>\u8fd9\u6761\u8bb0\u5f55\u7684\u53c2\u8003\u53ea\u6709\u4e00\u4e2a G\uff0c\u66ff\u4ee3\u5e8f\u5217\u7684\u5f00\u5934\u4e5f\u6709\u4e00\u4e2a G\u3002\u7b2c\u4e00\u4e2a\u5b57\u7b26\u662f\u5171\u4eab\u951a\u70b9\uff0c\u5c06\u5b83\u7559\u5728\u539f\u4f4d\uff0c\u518d\u53d6\u66ff\u4ee3\u5e8f\u5217\u53f3\u8fb9\u591a\u51fa\u6765\u7684\u90e8\u5206\uff0c\u6b63\u597d\u5f97\u5230\u4e8b\u5148\u8bbe\u8ba1\u7684\u4e8c\u5341\u56db\u4e2a\u78b1\u57fa\u3002\u628a\u6574\u6761\u66ff\u4ee3\u5e8f\u5217\u957f\u5ea6\u5f53\u4f5c\u63d2\u5165\u957f\u5ea6\uff0c\u4f1a\u591a\u7b97\u4e00\u4e2a\u3002\u63a5\u7740\u53d6\u8bb0\u5f55\u7ed9\u51fa\u7684\u7ec4\u88c5\u5e8f\u5217\u540d\u79f0\uff0c\u5728\u7ec4\u88c5\u6bd4\u5bf9\u6587\u4ef6\u91cc\u627e\u5230\u540c\u540d\u6761\u76ee\u3002\u5b83\u4ece\u53c2\u8003\u7684\u7b2c 9905 \u4f4d\u5f00\u59cb\uff0c\u5148\u8d70\u4e5d\u5341\u516d\u4e2a\u5339\u914d\u64cd\u4f5c\uff0c\u518d\u63d2\u5165\u4e8c\u5341\u56db\u4e2a\u78b1\u57fa\uff0c\u6700\u540e\u53c8\u8d70\u4e00\u767e\u4e2a\u5339\u914d\u64cd\u4f5c\u3002\u56e0\u6b64\u63d2\u5165\u524d\u6700\u540e\u4e00\u4e2a\u53c2\u8003\u4f4d\u7f6e\u4e3a\u4e00\u4e07\uff0c\u548c\u53d8\u5f02\u8868\u72ec\u7acb\u5bf9\u4e0a\u3002<\/p>\n<div class=\"guide-table\" style=\"max-width:100%;overflow-x:auto;margin:18px 0\">\n<table style=\"display:table;width:100%;max-width:100%;min-width:0;table-layout:fixed;border-collapse:collapse;font-size:.92em;line-height:1.6\">\n<thead>\n<tr>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#fff1e9;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u590d\u6838\u5165\u53e3<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#fff1e9;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u672c\u4f8b\u7684\u8f93\u51fa<\/th>\n<th scope=\"col\" style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;background:#fff1e9;color:#1e293b;vertical-align:top;text-align:left;padding:6px;border:1px solid #c4cfdb\">\u80fd\u786e\u8ba4\u7684\u4e8b\u60c5<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u4f53\u7ec6\u80de\u5c0f\u53d8\u5f02 VCF<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\"><code style=\"white-space:normal;overflow-wrap:anywhere;word-break:break-word;font-size:.88em\">POS=10000<\/code>\uff0c<code style=\"white-space:normal;overflow-wrap:anywhere;word-break:break-word;font-size:.88em\">REF=G<\/code>\uff0c<code style=\"white-space:normal;overflow-wrap:anywhere;word-break:break-word;font-size:.88em\">SPAN=24<\/code><\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u4f4d\u7f6e\u91c7\u7528\u63d2\u5165\u524d\u7684\u53c2\u8003\u951a\u70b9<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u5c40\u90e8\u7ec4\u88c5\u5e8f\u5217<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\"><code style=\"white-space:normal;overflow-wrap:anywhere;word-break:break-word;font-size:.88em\">96M24I100M<\/code>\uff0c<code style=\"white-space:normal;overflow-wrap:anywhere;word-break:break-word;font-size:.88em\">MAPQ=60<\/code><\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u63d2\u5165\u5de6\u53f3\u90fd\u6709\u7ec4\u88c5\u5e8f\u5217\u6bd4\u56de\u53c2\u8003<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u80bf\u7624\u6837\u672c<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\"><code style=\"white-space:normal;overflow-wrap:anywhere;word-break:break-word;font-size:.88em\">GT=0\/1, AD=24, DP=39, SR=24, CR=5<\/code><\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u6709\u66ff\u4ee3\u652f\u6301\uff0c\u5b57\u6bb5\u8981\u5206\u522b\u89e3\u91ca<\/td>\n<\/tr>\n<tr>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u5339\u914d\u6b63\u5e38\u6837\u672c<\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\"><code style=\"white-space:normal;overflow-wrap:anywhere;word-break:break-word;font-size:.88em\">GT=0\/0, AD=0, DP=48, SR=0, CR=0<\/code><\/td>\n<td style=\"white-space:normal;overflow-wrap:anywhere;word-break:normal;padding:6px;vertical-align:top;border:1px solid #c4cfdb\">\u5206\u6bcd\u53ef\u89c1\uff0c\u672c\u6b21\u672a\u89c1\u66ff\u4ee3\u652f\u6301<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<figure class=\"guide-figure\" style=\"margin:24px 0 28px\"><img loading=\"lazy\" decoding=\"async\" src=\"\/wordpress\/wp-content\/uploads\/2026\/11\/svaba-local-assembly-24bp-tumor-insertion-guide.png\" alt=\"\u8ffd\u8e2a 24 bp \u4f53\u7ec6\u80de\u63d2\u5165\u3002\u540c\u4e00\u5bf9\u80bf\u7624\u548c\u6b63\u5e38\u6587\u5e93\uff0c\u5f00\u542f\u5c40\u90e8\u7ec4\u88c5\u540e\u5b9a\u4f4d\u5230\u8bbe\u8ba1\u7684\u63a5\u5934\u3002\u53d8\u5f02\u8868\uff0c\u4e00\u4e2a\u63d2\u5165\uff0c\u7b2c 10000 \u4f4d\u7684 G \u662f\u5171\u4eab\u951a\u70b9\uff0cALT \u6bd4 REF \u591a 24 \u4e2a\u78b1\u57fa\u3002\u7ec4\u88c5\u5e8f\u5217\uff0c96M24I100M\uff0c\u4ece 9905 \u5f00\u59cb\uff0c\u5de6\u4fa7 96 \u4e2a\u5339\u914d\u64cd\u4f5c\u6b63\u597d\u8d70\u5230 10000\u3002\u6837\u672c\u8bc1\u636e\uff0c\u80bf\u7624 24 \/ \u6b63\u5e38 0\uff0c\u4e24\u4e2a\u6837\u672c\u7684\u603b\u6df1\u5ea6\u5206\u522b\u4e3a 39 \u4e0e 48\u3002\" width=\"1200\" height=\"840\" style=\"display:block;width:100%;height:auto;border-radius:10px\"><figcaption style=\"font-size:14px;line-height:1.6;margin:9px 2px 0;color:#475569\">\u540c\u4e00\u5bf9\u80bf\u7624\u548c\u6b63\u5e38\u6587\u5e93\uff0c\u5f00\u542f\u5c40\u90e8\u7ec4\u88c5\u540e\u5b9a\u4f4d\u5230\u8bbe\u8ba1\u7684\u63a5\u5934\u3002<\/figcaption><\/figure>\n<h2 style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 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style=\"font-size:1.38em;line-height:1.5;margin:1.55em 0 .55em;color:#182d43\">\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u6ca1\u6709\u5019\u9009\u65f6\u5148\u67e5\u5b83\u6d88\u5931\u5728\u54ea\u4e00\u6b65<\/h2>\n<pre style=\"max-width:100%;box-sizing:border-box;overflow-x:auto;margin:18px 0;padding:14px 16px;background:#f3f6fa;color:#1a293c;border:1px solid #d8e1ea;border-radius:8px;line-height:1.55;white-space:pre;overflow-wrap:normal;word-break:normal\"><code class=\"language-bash\">svaba run -t TUMOR.bam -n NORMAL.bam -G ref.fa -a demo -p 1 -k chrToy:200-19600\r\nsvaba run -t TUMOR.bam -n NORMAL.bam -G ref.fa -a pairs -p 1 -k chrToy:200-19600 --discordant-only\r\ngrep -v '^#' demo.svaba.somatic.indel.vcf\r\nsamtools view demo.contigs.bam | cut -f1-6\r\n<\/code><\/pre>\n<p>\u4ec5\u770b\u5f02\u5e38\u914d\u5bf9\u7684\u90a3\u4e00\u8f6e\uff0c\u516b\u4efd\u53d8\u5f02\u8868\u5168\u90e8\u4e3a\u7a7a\uff0c\u8fde\u672a\u8fc7\u6ee4\u7684\u5c0f\u53d8\u5f02\u6587\u4ef6\u4e5f\u6ca1\u6709\u8fd9\u4e2a\u4f4d\u7f6e\u3002\u56e0\u6b64\u8fd9\u6b21\u5dee\u5f02\u53d1\u751f\u5728\u5019\u9009\u751f\u6210\u9636\u6bb5\u3002\u4e8c\u5341\u56db\u78b1\u57fa\u63d2\u5165\u53ef\u4ee5\u88ab\u8de8\u8fc7\u63a5\u5934\u7684\u8bfb\u6bb5\u76f4\u63a5\u8986\u76d6\uff0c\u7247\u6bb5\u957f\u5ea6\u672c\u8eab\u53c8\u6709\u5206\u5e03\uff0c\u63d2\u5165\u5e76\u4e0d\u4fdd\u8bc1\u7ed9\u914d\u5bf9\u8ddd\u79bb\u5e26\u6765\u660e\u663e\u5f02\u5e38\u3002\u628a\u4e00\u4e2a\u6ca1\u6709\u8fdb\u5165\u5019\u9009\u5217\u8868\u7684\u4e8b\u4ef6\u53cd\u590d\u8c03\u4f4e\u6700\u7ec8\u8fc7\u6ee4\u95e8\u69db\uff0c\u65e0\u6cd5\u627e\u56de\u8fd9\u4e00\u8f6e\u4ece\u672a\u6784\u5efa\u7684\u90a3\u6761\u7ec4\u88c5\u5e8f\u5217\u3002\u8fd9\u91cc\u7528\u7684\u662f\u5355\u4e00\u3001\u65e0\u91cd\u590d\u80cc\u666f\u4e0b\u7684\u4eba\u5de5\u63d2\u5165\uff0c\u4e0d\u8db3\u4ee5\u4f30\u8ba1\u4e24\u79cd\u6a21\u5f0f\u5728\u590d\u6742\u4eba\u7c7b\u57fa\u56e0\u7ec4\u4e0a\u7684\u68c0\u51fa\u7387\u3002<\/p>\n<p>\u56de\u5230\u771f\u5b9e\u6570\u636e\u65f6\uff0c\u53ef\u4ee5\u5148\u5bfc\u51fa\u5019\u9009\u4e24\u4fa7\u7684\u4e00\u5c0f\u6bb5\u53c2\u8003\uff0c\u590d\u67e5\u63d2\u5165\u7684\u5168\u90e8\u5e8f\u5217\u548c\u4e24\u4fa7\u53ef\u552f\u4e00\u5b9a\u4f4d\u7684\u957f\u5ea6\u3002\u518d\u770b\u540c\u4e00\u4f4d\u7f6e\u6709\u6ca1\u6709\u7b2c\u4e8c\u4e2a\u8fd1\u4f3c\u7684\u6bd4\u5bf9\u843d\u70b9\uff0c\u6216\u8005\u4e00\u4fa7\u53ea\u6709\u6781\u77ed\u7684\u951a\u5b9a\u3002\u9047\u5230\u4e32\u8054\u91cd\u590d\u65f6\uff0c\u4e0d\u540c\u8f6f\u4ef6\u53ef\u80fd\u628a\u7b49\u4ef7\u63d2\u5165\u653e\u5728\u76f8\u90bb\u5750\u6807\uff0c\u6b64\u65f6\u76f4\u63a5\u6c42\u5750\u6807\u4ea4\u96c6\u4f1a\u6f0f\u6389\u540c\u4e00\u4e8b\u4ef6\u3002\u91cd\u65b0\u62fc\u51fa\u4e24\u8fb9\u7684\u66ff\u4ee3\u5355\u500d\u578b\u5e76\u6bd4\u8f83\u5e8f\u5217\uff0c\u6bd4\u53ea\u770b\u4e00\u884c\u662f\u5426\u6807\u8bb0\u901a\u8fc7\u66f4\u80fd\u56de\u7b54\u4e24\u4efd\u7ed3\u679c\u5230\u5e95\u662f\u5426\u4e00\u81f4\u3002\u4fdd\u7559\u5c40\u90e8\u7ec4\u88c5\u5e8f\u5217\u548c\u6837\u672c\u5b57\u6bb5\u5934\uff0c\u5f80\u5f80\u80fd\u8ba9\u65e5\u540e\u7684\u590d\u5ba1\u5c11\u8d70\u597d\u51e0\u6b65\u3002<\/p>\n<p 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CIGAR\u3001\u53d8\u5f02\u7b49\u4f4d\u548c\u914d\u5bf9\u6b63\u5e38\u6837\u672c\u590d\u6838\u4e00\u4e2a\u5df2\u77e5\u63d2\u5165\u3002<\/p>\n","protected":false},"author":1,"featured_media":70436,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[49],"tags":[1023,986,1024,1025],"class_list":["post-70437","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-svaba","tag-986","tag-1024","tag-1025"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.biocloudservice.com\/wordpress\/wp-content\/uploads\/2026\/11\/svaba-local-assembly-24bp-tumor-insertion-guide.png?fit=1200%2C840&ssl=1","_links":{"self":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70437","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/comments?post=70437"}],"version-history":[{"count":1,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70437\/revisions"}],"predecessor-version":[{"id":70447,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/posts\/70437\/revisions\/70447"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media\/70436"}],"wp:attachment":[{"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/media?parent=70437"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/categories?post=70437"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biocloudservice.com\/wordpress\/wp-json\/wp\/v2\/tags?post=70437"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}