{"id":70562,"date":"2026-11-14T23:54:53","date_gmt":"2026-11-14T15:54:53","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=70562"},"modified":"2026-11-14T23:54:53","modified_gmt":"2026-11-14T15:54:53","slug":"racon-unpolished-contigs-window-trimming-coverage","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/racon-unpolished-contigs-window-trimming-coverage\/","title":{"rendered":"Racon \u5b9e\u8dd1\uff5c\u4e24\u5904\u9519\u78b1\u57fa\u4fee\u597d\u4e86\uff0c\u4e3a\u4ec0\u4e48\u4e00\u6761 contig \u6d88\u5931\uff0c\u53e6\u4e00\u6761\u77ed\u4e86 400 bp\uff1f"},"content":{"rendered":"
\n

\u505a\u5b8c\u4e00\u8f6e\u7ec4\u88c5\u629b\u5149\uff0c\u539f\u6765\u4e24\u4e2a\u6000\u7591\u6709\u9519\u7684\u4f4d\u70b9\u90fd\u5f97\u5230\u4e86\u7ea0\u6b63\u3002\u51c6\u5907\u4ea4\u4ed8\u65f6\uff0c\u5374\u53d1\u73b0\u603b\u5e8f\u5217\u957f\u5ea6\u4e5f\u53d8\u4e86\u3002\u5148\u522b\u628a\u5dee\u503c\u90fd\u5199\u6210\u7ec4\u88c5\u9519\u8bef\u88ab\u4fee\u590d\u3002Racon \u53ef\u4ee5\u6539\u5199\u6709\u8bfb\u6bb5\u652f\u6301\u7684\u5c40\u90e8\u5171\u8bc6\uff0c\u9ed8\u8ba4\u884c\u4e3a\u8fd8\u4f1a\u5f71\u54cd\u54ea\u4e9b\u5e8f\u5217\u51fa\u73b0\u5728\u8f93\u51fa\u6587\u4ef6\u91cc\uff0c\u4ee5\u53ca\u7a97\u53e3\u8fb9\u754c\u600e\u6837\u4fee\u526a\u3002\u8fd9\u4e09\u4e2a\u95ee\u9898\u53ef\u4ee5\u5206\u522b\u5236\u9020\u5bf9\u7167\u3002\u6211\u4eec\u9020\u4e86\u4e24\u6761\u957f\u5ea6\u5df2\u77e5\u7684\u8349\u56fe\uff0c\u53ea\u7ed9\u5176\u4e2d\u4e00\u6761\u7684\u524d\u90e8\u63d0\u4f9b\u8bfb\u6bb5\uff0c\u5e76\u628a\u4e09\u4e2a\u4eba\u5de5\u9519\u8bef\u57cb\u5728\u4e0d\u540c\u6df1\u5ea6\u5904\u3002\u8fd9\u6837\u8f93\u51fa\u91cc\u7684\u6bcf\u4e2a\u53d8\u5316\u90fd\u80fd\u56de\u5230\u5177\u4f53\u7684\u539f\u59cb\u78b1\u57fa\u3002Racon \u9879\u76ee\u8bf4\u660e<\/a><\/p>\n

\u4e09\u4efd\u8f93\u5165\u5728\u751f\u7269\u5b66\u4e0a\u5fc5\u987b\u5bf9\u5e94\u8d77\u6765<\/h2>\n

Racon \u9700\u8981\u63d0\u4f9b\u6d4b\u5e8f\u8bfb\u6bb5\u3001\u8bfb\u6bb5\u5bf9\u76ee\u6807\u5e8f\u5217\u7684\u6bd4\u5bf9\uff0c\u4ee5\u53ca\u5f85\u629b\u5149\u7684\u76ee\u6807\u5e8f\u5217\u3002\u6b21\u5e8f\u6709\u5b9e\u9645\u542b\u4e49\u3002\u6bd4\u5bf9\u91cc\u7684\u67e5\u8be2\u540d\u79f0\u5fc5\u987b\u80fd\u5728\u8bfb\u6bb5\u6587\u4ef6\u4e2d\u627e\u5230\uff0c\u76ee\u6807\u540d\u79f0\u4e0e\u5750\u6807\u5e94\u5f53\u5bf9\u5e94\u5f53\u524d\u8fd9\u7248\u7ec4\u88c5\u3002\u591a\u4e2a\u9879\u76ee\u90fd\u7528\u7c7b\u4f3c\u7684\u7247\u6bb5\u540d\u65f6\uff0c\u6bd4\u5bf9\u6587\u4ef6\u5bb9\u6613\u7528\u9519\uff0c\u56e0\u6b64\u9664\u4e86\u67e5\u540d\u5b57\uff0c\u6700\u597d\u4e5f\u67e5\u76ee\u6807\u957f\u5ea6\u3001\u53c2\u8003\u6587\u4ef6\u6821\u9a8c\u503c\u548c\u4e0a\u4e00\u8f6e\u4fee\u6539\u65e5\u671f\u3002\u505a\u7b2c\u4e8c\u8f6e\u629b\u5149\u9700\u8981\u91cd\u65b0\u628a\u8bfb\u6bb5\u6bd4\u56de\u7b2c\u4e00\u8f6e\u7684\u4ea7\u7269\u3002\u53ea\u8981\u67d0\u4e2a\u7a97\u53e3\u53d1\u751f\u957f\u5ea6\u53d8\u5316\uff0c\u539f\u6bd4\u5bf9\u7684\u540e\u7eed\u5750\u6807\u5c31\u53ef\u80fd\u5e73\u79fb\uff0c\u5373\u4f7f\u6807\u9898\u5b8c\u5168\u4e00\u6837\uff0c\u4e5f\u4e0d\u80fd\u628a\u4e0a\u4e00\u8f6e\u7684\u5750\u6807\u76f4\u63a5\u632a\u7528\u3002<\/p>\n

\u5b9e\u9a8c\u7684\u7b2c\u4e00\u6761\u8349\u56fe\u957f\u516d\u5343\u56db\u767e\u4e2a\u78b1\u57fa\uff0c\u501f\u52a9\u5df2\u77e5\u539f\u5e8f\u5217\uff0c\u6211\u4eec\u5728\u7b2c\u4e00\u5343\u3001\u4e8c\u5343\u4e94\u767e\u548c\u516d\u5343\u4f4d\u5404\u5236\u9020\u4e00\u4e2a\u66ff\u6362\u3002\u7b2c\u4e8c\u6761\u8349\u56fe\u957f\u4e00\u5343\u516d\u767e\u4e2a\u78b1\u57fa\uff0c\u5e8f\u5217\u6b63\u786e\uff0c\u4f46\u4e0d\u7ed9\u5b83\u4efb\u4f55\u8bfb\u6bb5\u3002\u8f93\u5165\u5171\u6709\u4e03\u5341\u4e8c\u6761\u957f\u5ea6\u5747\u4e3a\u4e00\u5343\u4e8c\u767e\u7684\u65e0\u9519\u8bfb\u6bb5\uff0c\u5747\u6765\u81ea\u7b2c\u4e00\u6761\u7684\u524d\u90e8\u3002\u5b9e\u9645\u7528 minimap2 \u6bd4\u5bf9\u540e\u7559\u4e0b\u4e03\u5341\u4e8c\u6761\u8bb0\u5f55\uff0c\u6700\u5de6\u8d77\u70b9\u4e3a\u96f6\uff0c\u6700\u53f3\u7ec8\u70b9\u4e3a\u56db\u5343\u4e03\u767e\u96f6\u4e09\uff0c\u6309\u5de6\u95ed\u53f3\u5f00\u7684\u5750\u6807\u89e3\u91ca\u3002\u524d\u4e24\u5904\u9519\u8bef\u786e\u5b9e\u6709\u8bfb\u6bb5\u8de8\u8fc7\uff0c\u6700\u8fdc\u7684\u4e00\u5904\u5728\u5168\u90e8\u8bfb\u6bb5\u7684\u8986\u76d6\u4e4b\u5916\u3002\u8fd9\u4e2a\u5b89\u6392\u5c06\u201c\u78b1\u57fa\u53ef\u80fd\u6709\u9519\u201d\u4e0e\u201c\u73b0\u6709\u89c2\u6d4b\u80fd\u5426\u7ea0\u6b63\u5b83\u201d\u5206\u5f00\u4e86\u3002<\/p>\n

\u628a\u8f93\u51fa\u6309\u540d\u5b57\u3001\u957f\u5ea6\u548c\u78b1\u57fa\u9010\u4e00\u6e05\u70b9<\/h2>\n

\u9ed8\u8ba4\u8fd0\u884c\u53ea\u8f93\u51fa\u7b2c\u4e00\u6761\uff0c\u957f\u5ea6\u4ece\u516d\u5343\u56db\u767e\u964d\u4e3a\u516d\u5343\u3002\u6211\u4eec\u6309\u5e8f\u5217\u6bd4\u5bf9\u5c06\u8f93\u51fa\u91cd\u65b0\u653e\u56de\u539f\u5750\u6807\uff0c\u53d1\u73b0\u524d\u4e24\u5904\u66ff\u6362\u90fd\u7ea0\u6b63\u4e86\u3002\u672a\u8986\u76d6\u7684\u7b2c\u516d\u5343\u4f4d\u4f9d\u65e7\u4fdd\u7559\u8349\u56fe\u91cc\u7684 A\uff0c\u5df2\u77e5\u771f\u503c\u4e3a C\u3002\u957f\u5ea6\u5dee\u843d\u5728\u4e24\u5904\uff0c\u4e00\u5904\u662f\u539f\u5e8f\u5217\u5f00\u5934\u7684\u4e00\u767e\u96f6\u4e09\u4e2a\u78b1\u57fa\uff0c\u53e6\u4e00\u5904\u662f\u7d27\u63a5\u6700\u540e\u6709\u8bfb\u6bb5\u8986\u76d6\u7684\u4f4d\u7f6e\u4e4b\u540e\u7684\u4e8c\u767e\u4e5d\u5341\u4e03\u4e2a\u78b1\u57fa\u3002\u4e24\u5904\u76f8\u52a0\u56db\u767e\uff0c\u65e2\u89e3\u5f00\u4e86\u7ec4\u88c5\u957f\u5ea6\u7684\u53d8\u5316\uff0c\u4e5f\u89e3\u91ca\u4e86\u539f\u6765\u7b2c\u516d\u5343\u4f4d\u4e3a\u4f55\u51fa\u73b0\u5728\u8f93\u51fa\u7684\u7b2c\u4e94\u5343\u516d\u767e\u4f4d\u3002\u628a\u524d\u540e\u4e24\u884c FASTA \u7684\u540c\u4e00\u5217\u6a2a\u7740\u6bd4\u8f83\uff0c\u4f1a\u628a\u4e00\u6b21\u88c1\u5207\u8bef\u8bfb\u6210\u5b83\u540e\u9762\u5927\u91cf\u78b1\u57fa\u5168\u90fd\u9519\u8bef\u3002<\/p>\n

\u7b2c\u4e8c\u8f6e\u5728\u5b8c\u5168\u76f8\u540c\u7684\u8bfb\u6bb5\u3001\u6bd4\u5bf9\u548c\u8349\u56fe\u4e0a\u989d\u5916\u5f00\u542f\u8f93\u51fa\u672a\u629b\u5149\u76ee\u6807\u7684\u9009\u9879\u3002\u65e0\u4efb\u4f55\u8bfb\u6bb5\u8986\u76d6\u7684\u7b2c\u4e8c\u6761\u56de\u6765\u4e86\uff0c\u957f\u5ea6\u4ecd\u4e3a\u4e00\u5343\u516d\u767e\uff0c\u4e0e\u539f\u5e8f\u5217\u9010\u5b57\u4e00\u81f4\u3002\u7b2c\u4e00\u6761\u4f9d\u65e7\u662f\u516d\u5343\uff0c\u90a3\u56db\u767e\u4e2a\u78b1\u57fa\u5e76\u6ca1\u6709\u4e00\u8d77\u56de\u6765\u3002\u7b2c\u4e09\u8f6e\u4fdd\u7559\u8fd9\u4e2a\u5f00\u5173\uff0c\u518d\u5173\u95ed\u7a97\u53e3\u7aef\u90e8\u4fee\u526a\u3002\u73b0\u5728\u7b2c\u4e00\u6761\u6062\u590d\u4e3a\u516d\u5343\u56db\u767e\uff0c\u7b2c\u4e8c\u6761\u4e5f\u4fdd\u7559\uff0c\u524d\u4e24\u5904\u9519\u8bef\u4ecd\u88ab\u6539\u6b63\uff0c\u7b2c\u4e09\u5904\u4ecd\u4e3a\u539f\u6765\u7684\u9519\u8bef\u5b57\u6bcd\u3002\u4e09\u4e2a\u7ed3\u679c\u653e\u5728\u4e00\u8d77\uff0c\u53ef\u4ee5\u5206\u522b\u8ffd\u95ee\u662f\u54ea\u4e00\u4e2a\u5f00\u5173\u6539\u53d8\u4e86\u5e8f\u5217\u6210\u5458\uff0c\u54ea\u4e00\u4e2a\u6539\u53d8\u4e86\u7b2c\u4e00\u6761\u5e8f\u5217\u7684\u957f\u5ea6\u3002Racon \u53c2\u6570\u4e0e\u7a97\u53e3\u4fee\u526a\u8bf4\u660e<\/a><\/p>\n

\n\n\n\n\n\n\n\n
\u540c\u4e00\u7ec4\u6570\u636e\u7684\u53c2\u6570<\/th>\n\u6709\u8bfb\u6bb5\u76ee\u6807<\/th>\n\u5b8c\u5168\u65e0\u8bfb\u6bb5\u76ee\u6807<\/th>\n\u539f\u7b2c 6000 \u4f4d<\/th>\n<\/tr>\n<\/thead>\n
\u9ed8\u8ba4<\/td>\n6000 bp<\/td>\n\u65e0\u8f93\u51fa<\/td>\n\u4ecd\u4e3a A\uff0c\u8f93\u51fa\u7b2c 5600 \u4f4d<\/td>\n<\/tr>\n
\u52a0 -u<\/code><\/td>\n6000 bp<\/td>\n1600 bp<\/td>\n\u4ecd\u4e3a A\uff0c\u8f93\u51fa\u7b2c 5600 \u4f4d<\/td>\n<\/tr>\n
\u52a0 -u --no-trimming<\/code><\/td>\n6400 bp<\/td>\n1600 bp<\/td>\n\u4ecd\u4e3a A\uff0c\u8f93\u51fa\u7b2c 6000 \u4f4d<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"\u6d88\u5931\u7684
\u540c\u4e00\u4efd\u8349\u56fe\u5206\u4e09\u6b21\u629b\u5149\uff0c\u9010\u6bb5\u6838\u5bf9\u6709\u8986\u76d6\u3001\u65e0\u8986\u76d6\u548c\u7a97\u53e3\u8fb9\u7f18\u3002<\/figcaption><\/figure>\n

\u6700\u7701\u4e8b\u7684\u62bd\u67e5\u4f4d\u7f6e\u6709\u4e09\u4e2a\uff0c\u5206\u522b\u6311\u5728\u9ad8\u8986\u76d6\u5185\u90e8\u3001\u8986\u76d6\u521a\u7ed3\u675f\u7684\u8fb9\u7f18\u3001\u6574\u6bb5\u6ca1\u6709\u8bfb\u6bb5\u5904\u3002\u5728\u540c\u4e00\u5f20\u8bb0\u5f55\u91cc\u5199\u51fa\u8349\u56fe\u78b1\u57fa\u3001\u7ea0\u6b63\u540e\u7684\u78b1\u57fa\u548c\u72ec\u7acb\u8bfb\u6bb5\u652f\u6301\uff0c\u518d\u53e6\u5916\u767b\u8bb0\u53c2\u8003\u5750\u6807\u662f\u5426\u5e73\u79fb\u3002\u8fd9\u6837\u5373\u4f7f\u5171\u8bc6\u6587\u4ef6\u6ca1\u6709\u9010\u78b1\u57fa\u8d28\u91cf\uff0c\u4e5f\u80fd\u6307\u51fa\u6bcf\u4e2a\u5224\u65ad\u4f9d\u8d56\u54ea\u4efd\u8bc1\u636e\u3002<\/p>\n

\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u5982\u4f55\u7ed9\u4e00\u6b21\u629b\u5149\u505a\u53ef\u89e3\u91ca\u7684\u9a8c\u6536<\/h2>\n
minimap2 -t 1 -x map-ont -c draft.fa reads.fq > overlaps.paf\r\nracon -t 1 reads.fq overlaps.paf draft.fa > default.fa\r\nracon -t 1 -u reads.fq overlaps.paf draft.fa > keep.fa\r\nracon -t 1 -u --no-trimming reads.fq overlaps.paf draft.fa > keep_full.fa\r\n<\/code><\/pre>\n
\u5148\u5bfc\u51fa\u524d\u540e\u4e24\u7248\u7684\u5e8f\u5217\u540d\u548c\u957f\u5ea6\uff0c\u5206\u522b\u67e5\u6574\u6761\u7f3a\u5931\u3001\u6574\u6761\u4ecd\u5728\u4f46\u7f29\u77ed\u3001\u957f\u5ea6\u76f8\u540c\u4f46\u78b1\u57fa\u6709\u6539\u53d8\u4e09\u79cd\u60c5\u51b5\u3002\u5bf9\u7b2c\u4e00\u79cd\u68c0\u67e5\u6709\u6ca1\u6709\u4efb\u4f55\u8bfb\u6bb5\u5bf9\u4e0a\uff0c\u518d\u67e5\u662f\u5426\u8981\u6c42\u4fdd\u7559\u672a\u629b\u5149\u5e8f\u5217\u3002\u5bf9\u7b2c\u4e8c\u79cd\u5148\u628a\u4e24\u7248\u76f8\u4e92\u6bd4\u5bf9\uff0c\u8ba9\u5dee\u503c\u843d\u5230\u539f\u5e8f\u5217\u7684\u67d0\u4e2a\u533a\u95f4\uff0c\u53e0\u52a0\u8bfb\u6bb5\u8986\u76d6\u7684\u7ec8\u70b9\u3002\u5bf9\u7b2c\u4e09\u79cd\u624d\u9002\u5408\u9010\u4f4d\u68c0\u67e5\u66ff\u6362\u662f\u5426\u5f97\u5230\u8bfb\u6bb5\u6216\u5176\u4ed6\u72ec\u7acb\u6570\u636e\u652f\u6301\u3002\u8bb0\u5f55\u6df1\u5ea6\u65f6\u7528\u5b9e\u9645\u5bf9\u9f50\u5230\u8be5\u5750\u6807\u7684\u78b1\u57fa\u8ba1\u6570\uff0c\u5728\u7a97\u53e3\u8fb9\u7f18\u8fd8\u8981\u67e5\u540c\u4e00\u7c07\u8bfb\u6bb5\u662f\u5426\u5168\u90fd\u5728\u9644\u8fd1\u540c\u65f6\u7ed3\u675f\u3002\u53ea\u6709\u51e0\u6761\u8bfb\u6bb5\u5ef6\u4f38\u5230\u8fb9\u7f18\uff0c\u548c\u5185\u90e8\u591a\u6761\u8bfb\u6bb5\u5b8c\u6574\u8de8\u8fc7\u7a97\u53e3\uff0c\u662f\u4e24\u79cd\u4e0d\u540c\u7684\u8bc1\u636e\u6761\u4ef6\u3002<\/p>\n
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\u9700\u8981\u66ff\u591a\u4efd\u7ec4\u88c5\u91cd\u590d\u6bd4\u5bf9\u4e0e\u629b\u5149\uff0c\u5e76\u4fdd\u7559\u5404\u8f6e\u5e8f\u5217\u548c\u8bc1\u636e\u65f6\uff0c\u53ef\u4f7f\u7528\u4e91\u751f\u4fe1\u7684\u751f\u7269\u4fe1\u606f\u5206\u6790\u670d\u52a1\u5668<\/a>\u7ba1\u7406\u540c\u4e00\u9879\u76ee\u7684\u591a\u4e2a\u7248\u672c\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
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