{"id":70867,"date":"2026-11-15T00:05:16","date_gmt":"2026-11-14T16:05:16","guid":{"rendered":"https:\/\/www.biocloudservice.com\/wordpress\/?p=70867"},"modified":"2026-11-15T00:05:16","modified_gmt":"2026-11-14T16:05:16","slug":"flye-plasmid-circularization-spanning-reads","status":"publish","type":"post","link":"https:\/\/www.biocloudservice.com\/wordpress\/flye-plasmid-circularization-spanning-reads\/","title":{"rendered":"Flye \u5b9e\u8dd1\uff5c\u8d28\u7c92\u5e73\u5747\u6df1\u5ea6\u8fd8\u6709 51 \u500d\uff0c\u4e3a\u4ec0\u4e48\u7ec4\u88c5\u7ed3\u679c\u4e0d\u518d\u6807\u4e3a\u73af\u72b6\uff1f"},"content":{"rendered":"
\n

\u505a\u7ec6\u83cc\u7ec4\u88c5\u65f6\uff0c\u4e00\u6761\u4e24\u4e07\u591a\u78b1\u57fa\u7684\u5e8f\u5217\u6709\u5f88\u9ad8\u7684\u6df1\u5ea6\uff0c\u76f8\u4f3c\u6027\u4e5f\u5f88\u597d\uff0c\u770b\u8d77\u6765\u50cf\u4e00\u6761\u5b8c\u6574\u8d28\u7c92\u3002\u80fd\u5426\u628a\u5b83\u753b\u6210\u5706\u73af\uff1f\u51b3\u5b9a\u6027\u7684\u8bc1\u636e\u5f80\u5f80\u85cf\u5728\u6700\u4e0d\u663e\u773c\u7684\u5730\u65b9\uff0c\u5373\u6709\u6ca1\u6709\u5355\u6761\u5206\u5b50\u771f\u6b63\u4ece\u5e8f\u5217\u7684\u672b\u7aef\u8de8\u5230\u5f00\u5934\u3002\u6211\u4eec\u7528 Flye \u505a\u4e86\u4e24\u4e2a\u4ece\u5934\u7ec4\u88c5\uff0c\u53ea\u4ece\u7b2c\u4e8c\u4efd\u6570\u636e\u4e2d\u62ff\u6389\u4e00\u7c7b\u8bfb\u6bb5\u3002\u5269\u4f59\u7684\u5c0f\u5206\u5b50\u6570\u636e\u4ecd\u8d85\u8fc7\u4e94\u5341\u500d\u8986\u76d6\uff0c\u5355\u770b\u5c40\u90e8\u5e8f\u5217\u5b8c\u5168\u6b63\u786e\uff0c\u7ec4\u88c5\u62a5\u544a\u7ed9\u51fa\u7684\u62d3\u6251\u5374\u53d8\u4e86\u3002\u4e24\u8f6e\u7684\u5927\u7247\u6bb5\u540c\u65f6\u5145\u5f53\u5bf9\u7167\uff0c\u80fd\u591f\u544a\u8bc9\u6211\u4eec\u6539\u53d8\u5177\u4f53\u53d1\u751f\u5728\u54ea\u91cc\u3002Flye \u9879\u76ee\u4e0e\u91cd\u590d\u56fe\u7ec4\u88c5\u8bf4\u660e<\/a><\/p>\n

\u5148\u5b9a\u4e49\u4ec0\u4e48\u53eb\u4e00\u6761\u8de8\u63a5\u8bfb\u6bb5<\/h2>\n

\u5b9e\u9a8c\u751f\u6210\u4e24\u4e2a\u65e0\u91cd\u590d\u8bbe\u8ba1\u7684\u968f\u673a\u73af\u72b6\u6a21\u677f\uff0c\u957f\u5ea6\u5206\u522b\u4e3a\u4e03\u4e07\u56db\u5343\u548c\u4e24\u4e07\u516d\u5343\u78b1\u57fa\u3002\u968f\u673a\u9009\u62e9\u8d77\u70b9\uff0c\u4ece\u5706\u4e0a\u8fde\u7eed\u53d6\u516d\u5343\u4e94\u767e\u81f3\u4e5d\u5343\u4e94\u767e\u4e2a\u78b1\u57fa\uff0c\u5947\u5076\u7f16\u53f7\u4ea4\u66ff\u4f7f\u7528\u6b63\u5411\u548c\u53cd\u5411\u3002\u82e5\u8d77\u70b9\u52a0\u8bfb\u957f\u8d85\u8fc7\u6a21\u677f\u957f\u5ea6\uff0c\u8bf4\u660e\u8fd9\u6761\u5206\u5b50\u5728\u5e8f\u5217\u6587\u4ef6\u9009\u5b9a\u7684\u5207\u70b9\u4e24\u8fb9\u90fd\u6709\u78b1\u57fa\uff0c\u65e0\u8bba\u6700\u540e\u628a\u5b83\u5199\u6210\u54ea\u4e2a\u65b9\u5411\uff0c\u90fd\u8bb0\u4e3a\u8de8\u63a5\u3002\u8fd9\u91cc\u7684\u5207\u70b9\u53ea\u662f\u4e3a\u5b58\u50a8\u73af\u72b6\u5e8f\u5217\u800c\u4efb\u610f\u9009\u62e9\u7684\u4e00\u5904\uff0c\u4e0d\u80fd\u628a\u5b83\u7406\u89e3\u6210\u7ec6\u80de\u4e2d\u771f\u5b9e\u5b58\u5728\u7684\u7269\u7406\u65ad\u53e3\u3002<\/p>\n

\u5927\u6a21\u677f\u62bd\u53d6\u56db\u767e\u4e09\u5341\u6761\uff0c\u5c0f\u6a21\u677f\u62bd\u53d6\u4e8c\u767e\u4e09\u5341\u516b\u6761\uff0c\u5176\u4e2d\u4e03\u5341\u4e00\u6761\u8de8\u8fc7\u5c0f\u6a21\u677f\u5207\u70b9\u3002\u7b2c\u4e00\u8f6e\u628a\u5168\u90e8\u516d\u767e\u516d\u5341\u516b\u6761\u4ea4\u7ed9 Flye 2.9.3\u3002\u7b2c\u4e8c\u8f6e\u4fdd\u7559\u5927\u6a21\u677f\u7684\u5168\u90e8\u8bfb\u6bb5\uff0c\u53ea\u5220\u9664\u5c0f\u6a21\u677f\u7684\u4e03\u5341\u4e00\u6761\u8de8\u63a5\u8bfb\u6bb5\uff0c\u6700\u540e\u5269\u4e94\u767e\u4e5d\u5341\u4e03\u6761\u3002\u4e24\u8f6e\u6700\u5c0f\u91cd\u53e0\u957f\u5ea6\u90fd\u8bbe\u4e3a\u4e24\u5343\uff0c\u4f7f\u7528\u540c\u4e00\u9ad8\u51c6\u786e\u7eb3\u7c73\u5b54\u8f93\u5165\u6a21\u5f0f\u4e0e\u76f8\u540c\u53c2\u6570\u3002\u8fd9\u91cc\u7684\u8bfb\u6bb5\u662f\u6839\u636e\u5df2\u77e5\u6a21\u677f\u751f\u6210\u7684\u65e0\u9519\u5e8f\u5217\uff0c\u9002\u5408\u89c2\u5bdf\u5c40\u90e8\u8986\u76d6\u3001\u95ed\u73af\u8bc1\u636e\u53ca\u6587\u4ef6\u8868\u793a\u4e4b\u95f4\u7684\u5173\u7cfb\u3002\u5b83\u4eec\u6ca1\u6709\u6a21\u62df\u771f\u5b9e\u6d4b\u5e8f\u4e2d\u7684\u540c\u805a\u7269\u8bef\u5dee\u548c\u8fd1\u7f18\u8d28\u7c92\u5171\u4eab\u91cd\u590d\uff0c\u4e0d\u80fd\u636e\u6b64\u4f30\u7b97\u5b9e\u9645\u9879\u76ee\u7684\u95ed\u73af\u6210\u529f\u7387\u3002<\/p>\n

\u957f\u5ea6\u3001\u5c40\u90e8\u8986\u76d6\u548c\u95ed\u73af\u4fe1\u53f7\u5206\u522b\u53bb\u54ea\u91cc\u8bfb<\/h2>\n

\u7b2c\u4e00\u8f6e\u7684\u7ec4\u88c5\u4fe1\u606f\u8868\u6709\u4e24\u4e2a\u7247\u6bb5\uff0c\u957f\u5ea6\u6070\u4e3a\u4e24\u6761\u6a21\u677f\u7684\u771f\u503c\uff0c\u73af\u72b6\u5217\u5747\u4e3a Y\u3002\u7b2c\u4e8c\u8f6e\u5927\u6a21\u677f\u4ecd\u5f97\u5230\u4e03\u4e07\u56db\u5343\u78b1\u57fa\uff0c\u4ecd\u7136\u6807\u8bb0\u4e3a\u73af\u72b6\uff0c\u5c0f\u6a21\u677f\u5219\u5f97\u5230\u4e8c\u4e07\u4e94\u5343\u4e03\u767e\u56db\u5341\u516b\u4e2a\u78b1\u57fa\uff0c\u73af\u72b6\u5217\u53d8\u4e3a N\u3002\u4e24\u8f6e\u7684\u7247\u6bb5\u7f16\u53f7\u8fd8\u4ea4\u6362\u4e86\u3002\u5982\u679c\u53ea\u628a\u7b2c\u4e00\u8f6e\u7684\u4e00\u53f7\u7247\u6bb5\u548c\u7b2c\u4e8c\u8f6e\u7684\u4e00\u53f7\u7247\u6bb5\u653e\u5728\u540c\u4e00\u884c\uff0c\u4f1a\u8bef\u4ee5\u4e3a\u539f\u6765\u5b8c\u6574\u7684\u5927\u7247\u6bb5\u4e00\u4e0b\u7f29\u77ed\u4e86\u6570\u4e07\u78b1\u57fa\u3002\u8de8\u8f6e\u6bd4\u8f83\u5e94\u5148\u6309\u5e8f\u5217\u6bd4\u56de\u5bf9\u5e94\u6a21\u677f\uff0c\u6216\u8005\u6309\u4e00\u7ec4\u53ef\u9760\u7684\u72ec\u7279\u6807\u8bb0\u786e\u8ba4\u8eab\u4efd\uff0c\u518d\u6bd4\u8f83\u957f\u5ea6\u4e0e\u62d3\u6251\u3002<\/p>\n

\u5c0f\u6a21\u677f\u5269\u4f59\u8bfb\u6bb5\u7684\u78b1\u57fa\u603b\u91cf\u9664\u4ee5\u771f\u5b9e\u6a21\u677f\u957f\u5ea6\uff0c\u7b2c\u4e00\u8f6e\u4e3a\u4e03\u5341\u4e09\u70b9\u4e09\u4e09\u500d\uff0c\u7b2c\u4e8c\u8f6e\u4ecd\u6709\u4e94\u5341\u4e00\u70b9\u4e09\u4e8c\u500d\u3002Flye \u7ed3\u679c\u8868\u7684\u5c0f\u7247\u6bb5\u8986\u76d6\u5217\u76f8\u5e94\u4e3a\u4e03\u5341\u4e03\u548c\u516d\u5341\u3002\u4e24\u4e2a\u6570\u503c\u7531\u4e0d\u540c\u65b9\u6cd5\u8ba1\u7b97\uff0c\u524d\u8005\u7528\u6a21\u62df\u5668\u8bb0\u5f55\u7684\u539f\u59cb\u603b\u4ea7\u91cf\uff0c\u540e\u8005\u662f\u8f6f\u4ef6\u5bf9\u7ec4\u88c5\u7247\u6bb5\u7684\u4f30\u8ba1\u3002\u7279\u522b\u662f\u5220\u9664\u8de8\u63a5\u8bc1\u636e\u540e\uff0c\u7ec4\u88c5\u7247\u6bb5\u672c\u8eab\u7684\u6709\u6548\u957f\u5ea6\u548c\u88ab\u8986\u76d6\u7684\u533a\u95f4\u4e5f\u53d8\u4e86\u3002\u4e00\u4e2a\u6570\u5b57\u5927\u4e8e\u4e94\u5341\u53ef\u4ee5\u548c\u672b\u7aef\u5b8c\u5168\u7f3a\u5c11\u8fde\u7eed\u8bc1\u636e\u540c\u65f6\u53d1\u751f\uff0c\u4e0d\u80fd\u7528\u5e73\u5747\u6df1\u5ea6\u66ff\u672b\u7aef\u6295\u7968\u3002Flye \u8f93\u51fa\u6587\u4ef6\u4e0e\u8986\u76d6\u5b57\u6bb5<\/a><\/p>\n

\n\n\n\n\n\n\n
\u8fdb\u5165\u7ec4\u88c5\u7684\u5c0f\u6a21\u677f\u8bfb\u6bb5<\/th>\n\u8de8\u5207\u70b9\u8bfb\u6bb5<\/th>\n\u5c0f\u6a21\u677f\u539f\u59cb\u5e73\u5747\u6df1\u5ea6<\/th>\n\u62fc\u51fa\u7684\u957f\u5ea6<\/th>\n\u73af\u72b6\u5217<\/th>\n<\/tr>\n<\/thead>\n
\u5168\u90e8 238 \u6761<\/td>\n71<\/td>\n73.33 \u500d<\/td>\n26000 bp<\/td>\nY<\/td>\n<\/tr>\n
\u5220\u9664\u8de8\u63a5\u540e\u7684 167 \u6761<\/td>\n0<\/td>\n51.32 \u500d<\/td>\n25748 bp<\/td>\nN<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\"51
\u4ece\u540c\u4e00\u6279\u8bfb\u6bb5\u4e2d\u79fb\u53bb\u8de8\u5c0f\u8d28\u7c92\u5207\u70b9\u7684 71 \u6761\uff0c\u5176\u4ed6\u8bfb\u6bb5\u4e0e\u53c2\u6570\u4fdd\u6301\u4e00\u81f4\u3002<\/figcaption><\/figure>\n

\u5728\u6837\u672c\u4ea4\u63a5\u65f6\uff0c\u53ef\u4ee5\u628a\u73af\u72b6\u5019\u9009\u5355\u72ec\u5bfc\u51fa\u4e00\u5f20\u8868\uff0c\u8bb0\u5f55\u5e8f\u5217\u540d\u3001\u957f\u5ea6\u3001\u7ec4\u88c5\u62a5\u544a\u7684\u73af\u72b6\u6807\u8bb0\u3001\u9996\u5c3e\u8fde\u63a5\u70b9\u548c\u652f\u6301\u8fde\u63a5\u7684\u72ec\u7acb\u8bfb\u540d\u3002\u82e5\u8bfb\u6bb5\u652f\u6301\u6765\u81ea\u540c\u4e00\u5904\u5f88\u77ed\u7684\u91cd\u590d\uff0c\u8868\u91cc\u9700\u8981\u53e6\u8bb0\u63a5\u5934\u4e24\u8fb9\u5404\u6709\u591a\u5c11\u72ec\u7279\u78b1\u57fa\u3002\u8fd9\u6837\u540e\u6765\u5373\u4f7f\u6709\u4eba\u628a\u65cb\u8f6c\u540e\u7684\u8d28\u7c92\u91cd\u65b0\u7f16\u53f7\uff0c\u4e5f\u53ef\u4ee5\u56de\u5230\u539f\u5206\u5b50\u590d\u6838\u3002<\/p>\n

\u5b9e\u8dd1\u7ecf\u9a8c\uff5c\u8ba9\u73af\u72b6\u5e8f\u5217\u7684\u8d77\u70b9\u53ef\u4ee5\u81ea\u7531\u65cb\u8f6c<\/h2>\n
flye --nano-hq reads.fa --out-dir assembly --genome-size 100k --threads 2 --min-overlap 2000\r\ncat assembly\/assembly_info.txt\r\nminimap2 -t 1 -x asm5 -c --secondary=no truth_twice.fa assembly\/assembly.fasta > check.paf\r\n<\/code><\/pre>\n
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